CN108781816A - A kind of Black Box Tracing epicormic branch cuttage rooting method - Google Patents
A kind of Black Box Tracing epicormic branch cuttage rooting method Download PDFInfo
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- CN108781816A CN108781816A CN201810752113.5A CN201810752113A CN108781816A CN 108781816 A CN108781816 A CN 108781816A CN 201810752113 A CN201810752113 A CN 201810752113A CN 108781816 A CN108781816 A CN 108781816A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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Abstract
The invention discloses a kind of Black Box Tracing epicormic branch cuttage rooting methods, it is examination material with Black Box Tracing, influence by different exogenous hormone concentration to its epicormic branch cuttage rooting efficiency has carried out overall merit based on Principal Component Analysis to the cutting experiment of each processing combination.The result shows that：Larger through various concentration exogenous hormone processing cuttage rooting character variation degree, wherein branch amount, level-one root radical, two level root radical and root long degree of variation are larger, up to 70% or more.2 characteristic roots are extracted through factorial analysis>1 principal component adds up variance contribution ratio up to 89.109%, from the point of view of comprehensive score, through 500 mgL‑1ABT1 comprehensive score highests, cuttings root system is most；Through 800 mgL‑1The cuttings of HORMONE TREATMENT is taken second place, rooting rate highest and height of seedling highest, and control CK rooting effect indices are minimum.Hormone concentration should be selectively configured in producing extension process according to nursery demand.
Description
Technical field
The invention belongs to agricultural technology fields, are related to a kind of Black Box Tracing epicormic branch cuttage rooting method.
Background technology
Black Box Tracing (Aronia melacarpa) belongs to rose family Sorbus, is to collect edible, medicinal, gardens and life
The rare tree of the values such as state.Fruit and its extract have outstanding health-care effect, to hearts such as heart disease, hypertension
Cranial vascular disease has special efficacy.It is widely used in medicine and functional food industry in European and American areas.Anthocyanidin, Huang in fruit
Ketone (fresh fruit content is up to 0.25%~0.35%), polyphenol are that content is highest in known plants.Fruit ties up life there are many also containing
The substances such as element and mineral element.Fruit can be processed into the food and beverage such as fruit juice, fruit wine, jam, can, preserved fruit.It introduces in China
Black Box Tracing economic tree nearly 20 years or so.Up to now, cultivated area about 133.3~166.7hm2.The seeds are economical
Remarkable benefit, at garden output per hectare up to 10~15t, the best fruiting period be averaged single plant yield reach 10kg, per hectare annual value of production 22~
300000 yuan.
Xinjiang is located in Eurasia innerland, belongs to typical arid-desert area, and habitat conditions is very fragile, ecological construction task
It is more heavy.Black Box Tracing drought resisting, cold tolerance are strong, you can resistance to -40 DEG C of low temperature, it also can be below precipitation 500mm
Area can be grown naturally, thus have both ecological benefits and economic benefit, as extraordinary non-wood forest culture, ecology recovery, rebuild with
And the promotion of ornamental value has a vast market foreground in urban afforestation.
Mainly there is easy to operate, the breeding cycle is short, breeding coefficient is high, cost is at low cost etc. by cutting propagation at present
Advantage.Forefathers mostly use variance analysis, Multiple range test etc. about the evaluation of epicormic branch cuttage rooting efficiency, it is contemplated that initial data
Unit of account is inconsistent, and Wang Xian etc. carries out each Rooting effect factor using Subordinate Function when to lily cuttage Character Evaluation
Weighted average, it is higher closer to 1 comprehensive score in closed interval (0-1) corresponding corresponding membership values, increase test result
Accuracy.Wang Shusheng etc. influences height to rooting effect with regard to each rootage factors in testing cuckoo epicormic branch cuttage and carries out variance
Analysis, preferable to cutting experiment 19 groups of hormone combinations carry out principal component analysis, and calculate comprehensive score, test result more subject to
Really comprehensively.Thus on the basis of traditional analysis, overall merit is carried out by Principal Component Analysis.
In terms of the research majority of Black Box Tracing cuttage concentrates on cottage method, such as producing, matrix for cuttings
Selection manages and protects process etc., but carries out the report of overall merit still to Black Box Tracing cutting experiment using exogenous hormone after inserting
It is rarely seen.
Invention content
The purpose of the present invention is to provide a kind of Black Box Tracing epicormic branch cuttage rooting methods.Using Subordinate Function pair
The quantity guiding principle of rooting effect carries out unification, considers shadow of each rooting effect index to comprehensive score ranking based on factorial analysis
It rings, filters out the hormone concentration gradient of optimum Black Box Tracing epicormic branch cuttage comprehensively, to be Black Box Tracing new
Being widely applied for boundary provides reliable reference frame.
Its specific technical solution is：
A kind of Black Box Tracing epicormic branch cuttage rooting method, includes the following steps：
Step 1 is adopted before the fringe time is selected in 07 ︰ 00 of early morning, is acquired 2 years breeder mother and tree current year is given birth to apple neonatal tress, downward from terminal bud
Clip, per 10~12cm of segment length, every section of cuttings retains 2 blades, and blade subtracts 1/2~1/3 according to leaf area size, upper incision
Mouth is truncation；
Step 2, exogenous hormone processing：No. 1 hormone concentration 500-800mgL of ABT-1, the HORMONE TREATMENT time is 10min；
One layer of plastic foil is covered in ceiling before step 3, cuttings cuttage, and covers double-layered sunshade net, greenhouse on the plastic film
Greenhouse is equipped with full exposure interval-automatic spraying device, and 3d before cuttage, matrix is loam, and carrying out intertillage to matrix loosens the soil, and increases soil
Earth gas permeability is used in combination 800 times of liquid of potassium permanganate to carry out disinfection processing matrix, slotting machine long 7m, wide 1m, east-west direction, and cuttage is adopted
With straight cutting method, seeding row spacing is 6cm × 8cm, depth 4cm.
Temperature of shed is controlled at 25~28 DEG C when step 4, cuttage, humidity is maintained at 75% or more, and blade crimps
When, use sprayer to carry out moisturizing to blade face immediately, and ensure that matrix moistens.
Further, cuttage early period (0~15d), by temperature of shed control at 25~30 DEG C, ground temperature is controlled at 16~23 DEG C,
Humidity is maintained at 70% or more, and temperature of shed is more than 30 DEG C, east and west sides plastic foil is started it is ventilated, at interval of 45min
Using automatic sprayer spray cooling, or use Portable spray device for blade moisturizing.Cuttage mid-term (15~40d) is big absolutely
Most cuttings have formed callus and adventitious root, gradually extend the cuttings ventilation and penetrating light time at this time, remove one layer of sunshade net,
Every 7d, sprays 1000 times " green plant spring " foliar fertilizer and supplement the nutrients.In the cuttage later stage, plastic foil is removed, water spray number is changed to 3h
Once, humiture in canopy is reduced, gradually removes sunshade net when root system color by turning brown in vain to outdoor transition, canopy is opened and practices seedling.
Compared with prior art, beneficial effects of the present invention are：
The present invention is examination material with Black Box Tracing, by different exogenous hormone concentration to its epicormic branch cuttage rooting efficiency
It influences, overall merit has been carried out to the cutting experiment of each processing combination based on Principal Component Analysis.The result shows that：Through various concentration
Exogenous hormone processing cuttage rooting character variation degree is larger, and wherein branch amount, level-one root radical, two level root radical and root long become
Off course degree is larger, up to 70% or more.2 characteristic roots are extracted through factorial analysis>1 principal component, accumulative variance contribution ratio reach
89.109%, from the point of view of comprehensive score, through 500mgL-1ABT1 comprehensive score highests, cuttings root system is most；Through 800mg
L-1The cuttings of HORMONE TREATMENT is taken second place, rooting rate highest and height of seedling highest, and control (CK) rooting effect indices are minimum.?
Hormone concentration should be selectively configured in production extension process according to nursery demand.
Specific implementation mode
Technical scheme of the present invention is described in more detail with reference to embodiment.
1 materials and methods
1.1 experimental field overviews
It is domestic that the test site is located at Xinjiang Uygur Autonomous Regions Changji Hui Autonomous Prefecture, Xinjiang Jimusar County, 6.5 DEG C of average temperature of the whole year, pole
Hold 38.3 DEG C of the highest temperature, 33.8 DEG C of Annual lowest climate temperature-, 7 22.6 DEG C of monthly mean temperatures, annual relative humidity 57%, nothing
Frost season 150d, annual precipitation 232.7mm are often accompanied by the appearance of the weather such as hot dry wind, arid, frost.
1.2 test material
For trying Black Box Tracing, introduced by Heihe City Russian Forestry scientific and technological cooperation garden.Adopting the fringe time is selected in 07 ︰ of early morning
Before 00,2 years breeder mother's tree current years raw apple neonatal tress is acquired, from the downward clip of terminal bud, per 10~12cm of segment length, every section of cuttings retains 2
Piece blade, blade subtract 1/2~1/3 according to leaf area size, and upper lower cut is truncation.For trying exogenous hormone ABT root-inducing powders 1
Number (ABT-1) is developed by China Forestry Science Research Institute.It is equipped with full exposure interval-automatic spraying device for examination greenhouse.
1.3 test method
1.3.1 experimental design
Using completely random district's groups experimental design, ABT-1 hormone concentration gradients separately design for 0 (CK), 200,300,
500、800mg·L-1, the HORMONE TREATMENT time is 10min.3 groups of repetitions, 35 plants of cuttings of every group of repetition cuttage are arranged in every group of processing.
Matrix is loam.
1.3.2 field management
For covering one layer of plastic foil in ceiling before examination cuttings cuttage, and double-layered sunshade net is covered on the plastic film.Before cuttage
3d carries out intertillage to matrix and loosens the soil, increases the gas permeability of soil, 800 times of liquid of potassium permanganate is used in combination to carry out disinfection processing matrix.It inserts
Bed long 7m, wide 1m, east-west direction.It is 6cm × 8cm that cuttage, which uses straight cutting method, seeding row spacing, and depth is about 4cm.It will be in canopy when cuttage
Temperature is controlled at 25~28 DEG C, and humidity is maintained at 75% or more, when blade crimps, and uses sprayer to carry out blade face immediately
Moisturizing, and ensure that matrix moistens.Cuttage early period (0~15d), by temperature of shed control at 25~30 DEG C, ground temperature control 16~
23 DEG C, humidity is maintained at 70% or more, and temperature of shed is more than 30 DEG C, east and west sides plastic foil is started it is ventilated, at interval of
45min uses automatic sprayer spray cooling, or uses Portable spray device for blade moisturizing.Cuttage mid-term (15~40d),
Most cuttings have formed callus and adventitious root, gradually extend the cuttings ventilation and penetrating light time at this time, remove one layer of screening
Screened postive sprays 1000 times " green plant spring " foliar fertilizer and supplements the nutrients every 7d.In the cuttage later stage, plastic foil is removed, water spray number changes
It is primary for 3h, humiture in canopy is reduced, gradually removes sunshade net when root system color by turning brown in vain to outdoor transition, canopy is opened and practices
Seedling.
1.3.3 the statistics of morphological parameters
After cuttage 4 months, the strain number for Black Box Tracing cuttage seeding of taking root is counted, while measuring the level-one root item of cuttings
The branch amount of number, two level root item number, level-one root root long, aerial part cuttings calculates rooting rate by the strain number taken root.
Level-one root item number is the item number that level-one root root long is more than 1cm；Two level root item number is that two level root root long is more than 0.5cm's
Item number；The overall length of level-one root root long=level-one root/level-one root item number；Rooting rate (%)=cuttings of taking root strain number/cuttage total strain number *
100；Height of seedling is height of the ground diameter to cuttings top；Stem thickness is the rugosity of ground diameter.
1.4 data analysis
Principal component analysis is carried out to data using Excel 2007 and 19.0 softwares of SPSS.Data result, which uses, is subordinate to letter
Number method is standardized, positive correlation index (1) formula, negatively correlated index (2) formula, and formula is as follows：
In formula, XinAnd X 'inRespectively refer to i-th of index in n-th of standard specimen initial data it is inverted after membership function
Value；UinRefer to the original measurement result of n-th of standard specimen, i-th of index；UimaxAnd UiminRespectively refer in standard specimen group i-th of index most
Big and minimum value.
Principal Component Analysis:The result of data normalization is subjected to principal component analysis, is extracted in rooting effect index
The principal component having a significant impact obtains the score value F of each principal componentjn, summarize each score value DnCalculate corresponding principal component contributor rate EjMake
For weight.Calculation formula is as follows:
DnFor the comprehensive scores for each sample rooting effect that Principal Component Analysis obtains；FjnFor n-th of sample, j-th of feature
The principal component score value of value > 1；M is characterized the principal component number of value > 1；EjFor the contribution rate of j-th of principal component.
2 results and analysis
Black fruit Sorbus alnifloria rooting effect under the processing of 2.1 hormon concentration
By 1 degree of variation of table it is found that 7 rooting effect indexs have different degrees of variation phenomenon.Wherein, branch
Number, level-one root radical, two level root radical, level-one root root long degree of variation are larger, and the coefficient of variation reaches 70% or more, height of seedling and stem
Rough error is different smaller, and the coefficient of variation is less than 30%.
1 Black Box Tracing rooting effect result of table
The principal component analysis of 2.2 hormon concentration Black Box Tracing rooting effects
2.2.1 data normalization
Since the measurement unit of rooting effect index is different, it is not easy to carry out data analysis.Before carrying out principal component analysis,
Data are standardized using Subordinate Function, the results are shown in Table 2.It is required according to the evaluation of rooting effect, 7 lifes
Root character shows as that value is higher, and rooting efficiency is better.
The lower Black Box Tracing rooting effect standardization result of 2 hormon concentration of table processing
Data after standardization are subjected to principal component analysis, 7 rooting effect index extractions go out 2 principal components, obtain it
Extract square loading matrix and cube rotation square loading matrix (table 3).The characteristic root expression extracted can represent original
The number of data information, the variance contribution ratio of principal component f1 and f2 are 52.406% and 36.703% respectively, and 2 principal components pair
The characteristic root answered is all higher than 1, meets analysis and requires.Therefore 2 principal components extracted can be used to take root instead of original 7
4 hormone concentration Gradient screenings of characteristic index pair.
Characteristic root, variance contribution ratio and the contribution rate of accumulative total of 3 principal component of table
2.2.2 factorial analysis
As shown in Table 4, which reflects that rooting effect index is higher to load in this principal component and symbol is positive product
It is 0.994,0.895 and 0.963 successively that matter index, which has branch amount, level-one root radical, two level root radical, load flexible strategy, load power
The higher and symbols of number be that have stem thickness, load flexible strategy be -0.740 for the index of quality born.Show that the 1st principal component mainly reflects is
The quality of cuttings root system quality.
Load value is higher in 2nd principal component and symbol is the positive index of quality level-one root root long, height of seedling and rooting rate, carries
Charge values are 0.783,0.888 and 0.851 respectively, and show that the 2nd principal component mainly reflects is the height of the length of root system, increment
With quantity of taking root.
4 postrotational principal component loading matrix of table
2.2.3 the lower cuttings overall merit of hormon concentration processing and scoring event
With extracting square root after the characteristic value of the principal component load value (table 4) of rooting effect target variable divided by corresponding principal component
Root obtains the coefficient i.e. feature vector corresponding to 2 each indexs of principal component, and feature vector is multiplied with the data after standardization,
It can be obtained the function expression of 2 principal components (ZX indicates the data after standardization in formula)：
F1==0.5206ZX1+0.494ZX2+0.5124ZX3+0.3729ZX4-0.2642ZX5-0.4492ZX6-
0.2768ZX7；
F2==-0.0986ZX1-0.3474ZX2-0.2511ZX3+0.5521ZX4+0.5879ZX5-0.4694ZX6+
0.5755ZX7。
In 2 expression formulas, ZX1For branch amount, ZX2For level-one root radical, ZX3For two level root radical, ZX4For level-one root root
Long, ZX5For height of seedling, ZX6For stem thickness, ZX7For rooting rate.
The ratio of the sum of total characteristic value of extracted principal component is accounted for as weight using the corresponding characteristic value of each principal component, you can
Comprehensive function fz=A is calculated1f1+A2f2,Wherein A1、A2Respectively represent 2 it is main at
The characteristic value divided.
5 each principal component scores of table, comprehensive score and sequence are compared
As shown in Table 5, the lower Black Box Tracing rooting effect comprehensive score fz of hormon concentration processing from high to low according to
It is secondary be 500,800,300,200mgL-1,CK.It can be seen that cuttage rooting effect can be significantly improved using exogenous hormone.
By principal component f1Ranking is it is found that 500mgL-1>300mg·L-1>200mg·L-1>800mg·L-1>CK, warp
500mg·L-1ABT1 processing cuttings root of hair quantity and branch quantity it is most.The cuttings root system quality performance of CK processing is poor, hair
Radical amount and branch amount are minimum.Illustrate using exogenous hormone can effectively improve Black Box Tracing cuttings take root quantity and point
Branch number.
By principal component f2Ranking is it is found that 800mgL-1>300mg·L-1>500mg·L-1>200mg·L-1>CK is used
800mg·L-1ABT1 HORMONE TREATMENT cuttings root long longest, rooting rate and height of seedling highest.And inserting without HORMONE TREATMENT (CK)
Fringe rooting rate is minimum, and root long is most short, and score is minimum.
3 conclusions and discussion
Both Subordinate Function and Principal Component Analysis organic combination are different from traditional cuttage analysis side by the experiment
Method, the comprehensive hormone concentration extracted most beneficial for Black Box Tracing rooting efficiency.The test result shows to be subordinate in use
Function method is unified on the basis of rooting effect unit, is based on factorial analysis, extracts 2 characteristic roots>1 principal component adds up side
Poor contribution rate is 89.109%, can reflect all information of initial data comprehensively.Through comprehensive score fzSequence, exogenous hormone is to life
Root affect trait score is 500mgL successively-1>800mg·L-1>300mg·L-1>200mg·L-1>CK, using 500mgL-1ABT1 handle Black Box Tracing cuttage rooting best results.The test result is also shown that common factor f1With 500mgL-1
ABT1 treatment effects it is best, the root of hair number of cuttings is in significant positive correlation with branch amount, shows to improve cuttings root system quality energy
Effectively facilitate the growth of aerial part young sprout.Common factor f2Then with 800mgL-1ABT1 treatment effects it is best, rooting percent
It is in significant correlation with root long, height of seedling.Hormone concentration can be selectively configured in production according to the demand of nursery.
The foregoing is only a preferred embodiment of the present invention, protection scope of the present invention is without being limited thereto, it is any ripe
Those skilled in the art are known in the technical scope of present disclosure, the letter for the technical solution that can be become apparent to
Altered or equivalence replacement are each fallen in protection scope of the present invention.
Claims (3)
1. a kind of Black Box Tracing epicormic branch cuttage rooting method, which is characterized in that include the following steps：
Step 1 is adopted before the fringe time is selected in 07 ︰ 00 of early morning, is acquired 2 years breeder mother and tree current year is given birth to apple neonatal tress, from the downward clip of terminal bud,
Per 10~12 cm of segment length, every section of cuttings retains 2 blades, and blade subtracts 1/2~1/3 according to leaf area size, and upper lower cut is equal
For truncation；
Step 2, exogenous hormone processing：500~800 mgL of ABT-1 hormone concentrations-1, the HORMONE TREATMENT time is 10 min；
One layer of plastic foil is covered in ceiling before step 3, cuttings cuttage, and covers double-layered sunshade net, greenhouse on the plastic film
It is equipped with full exposure interval-automatic spraying device, 3 d before cuttage, matrix is loam, and carrying out intertillage to matrix loosens the soil, and it is saturating to increase soil
Gas is used in combination 800 times of liquid of potassium permanganate to carry out disinfection processing matrix, and slotting machine grows 7 m, wide 1 m, east-west direction, and cuttage uses
Straight cutting method, seeding row spacing are the cm of 6 cm × 8, and depth is 4 cm；
Temperature of shed is controlled at 25 ~ 28 DEG C when step 4, cuttage, humidity is maintained at 75% or more, when blade crimps, and stands
Sprayer is used to carry out moisturizing to blade face, and ensures that matrix moistens.
2. Black Box Tracing epicormic branch cuttage rooting method according to claim 1, which is characterized in that cuttage early period, will
Temperature of shed control is at 25 ~ 30 DEG C, and ground temperature is controlled at 16 ~ 23 DEG C, and humidity is maintained at 70% or more, and temperature of shed is more than 30
DEG C, east and west sides plastic foil is started ventilated, uses automatic sprayer spray cooling at interval of 45 min, or using just
It is blade moisturizing to take formula sprayer；Cuttage mid-term, most cuttings have formed callus and adventitious root, have gradually prolonged at this time
The long cuttings ventilation and penetrating light time removes one layer of sunshade net, every 7 d, sprays 1 000 times " green plant spring " foliar fertilizer Replacement Battalion
It supports；The cuttage later stage removes plastic foil, and it is primary that water spray number is changed to 3 h, reduces humiture in canopy and works as root gradually to outdoor transition
Be color by turning brown in vain when, remove sunshade net, open canopy practice seedling.
3. Black Box Tracing epicormic branch cuttage rooting method according to claim 1, which is characterized in that described in step 2
ABT-1 hormone concentrations are 500 mgL-1。
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| JP2020119058A (en) * | 2019-01-21 | 2020-08-06 | 日本製紙株式会社 | Prediction method of rooting rate |
| CN112889504A (en) * | 2021-01-21 | 2021-06-04 | 辽宁省旱地农林研究所 | Method for raising seedlings of black chokeberry by cutting in greenhouse with turfy soil plug |
| CN114521402A (en) * | 2022-03-07 | 2022-05-24 | 江苏农牧科技职业学院 | Cutting propagation method for aronia melanocarpa |
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| CN104412814A (en) * | 2013-08-30 | 2015-03-18 | 浙江省农业科学院 | Cutting propagation method of aronia melanocarpa elliot |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109479541A (en) * | 2018-12-26 | 2019-03-19 | 武威凯弘利特色农产品开发有限公司 | A kind of Black Box Tracing cuttage breeding method |
| JP2020119058A (en) * | 2019-01-21 | 2020-08-06 | 日本製紙株式会社 | Prediction method of rooting rate |
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| CN112889504A (en) * | 2021-01-21 | 2021-06-04 | 辽宁省旱地农林研究所 | Method for raising seedlings of black chokeberry by cutting in greenhouse with turfy soil plug |
| CN114521402A (en) * | 2022-03-07 | 2022-05-24 | 江苏农牧科技职业学院 | Cutting propagation method for aronia melanocarpa |
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