CN108760420B - Sample pretreatment platform with ultrasonic extraction function - Google Patents
Sample pretreatment platform with ultrasonic extraction function Download PDFInfo
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- CN108760420B CN108760420B CN201810430579.3A CN201810430579A CN108760420B CN 108760420 B CN108760420 B CN 108760420B CN 201810430579 A CN201810430579 A CN 201810430579A CN 108760420 B CN108760420 B CN 108760420B
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- 238000002137 ultrasound extraction Methods 0.000 title claims abstract description 21
- 239000007788 liquid Substances 0.000 claims abstract description 63
- 238000012360 testing method Methods 0.000 claims abstract description 33
- 230000029087 digestion Effects 0.000 claims abstract description 23
- 238000002347 injection Methods 0.000 claims abstract description 19
- 239000007924 injection Substances 0.000 claims abstract description 19
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 17
- 238000004140 cleaning Methods 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000004506 ultrasonic cleaning Methods 0.000 claims 1
- 238000012545 processing Methods 0.000 abstract description 6
- 238000002474 experimental method Methods 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 27
- 239000000284 extract Substances 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000012898 sample dilution Substances 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 238000003889 chemical engineering Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
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- Analytical Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention belongs to the technical field of instruments and meters, and discloses a sample pretreatment platform with an ultrasonic extraction function, which comprises: the ultrasonic liquid filling device comprises a workbench, a liquid filling unit, a pump valve pipeline unit and an ultrasonic device, wherein the liquid filling unit comprises a three-dimensional mechanical arm and a liquid filling needle tube, and the liquid filling needle tube is arranged on the three-dimensional mechanical arm; the pump valve pipeline unit comprises a plurality of pump valve integrated machines, and each pump valve integrated machine is integrated with a distribution valve and an injection pump; the digestion liquid reagent bottle is connected with an inlet channel of the distribution valve, and the liquid adding needle tube is connected with an outlet channel of the distribution valve; the ultrasonic device comprises an ultrasonic pool and a test tube rack, wherein at least one test tube is arranged in the test tube rack, the test tube rack is placed in the ultrasonic pool, and the test tube rack is positioned below the liquid adding needle tube. The invention solves the problem that the functions of the sample processing platform in the prior art are not comprehensive enough, and meets the multi-aspect application requirements of experiments.
Description
Technical Field
The invention relates to the technical field of instruments and meters, in particular to a sample pretreatment platform with an ultrasonic extraction function.
Background
With the continuous progress of scientific technology and the continuous development of measurement control technology, the full-automatic sample processing platform is widely applied to various experimental fields such as environmental protection, quality inspection, drug inspection, pharmaceutical chemical industry and the like, and particularly, the precision, the speed and the intelligence of a liquid sample processing module are greatly improved. The full-automatic liquid sample processing platform can perform functions of sample dilution constant volume, standard solution addition, liquid quantitative distribution and the like, and can also calculate a configuration scheme systematically and automatically. These functions only involve the dilution of the sample or the addition of reagents, and the composition of the mixed reagents after processing cannot be further analyzed. In many fields of precision measurement and analysis of chemical engineering or food, not only full-automatic sample pretreatment, namely concentration change and reagent addition, but also further treatment of components and states after subsequent samples react is required.
There is certain promotion space in the aspect of sample preliminary treatment at present, and on the whole, the function of current pretreatment device is mainly to carry out simple preliminary treatment to the sample, still includes in addition to carrying out sample dilution, solution interpolation and liquid ration to the standard solution. The working efficiency and the precision of the products have certain improvement space, and the functions of the products are not comprehensive enough.
Disclosure of Invention
The embodiment of the application solves the problem that the functions of a sample processing platform in the prior art are not comprehensive enough by providing the sample pretreatment platform with the ultrasonic extraction function.
The embodiment of the application provides a sample pretreatment platform with function is drawed to supersound, includes: the ultrasonic liquid filling device comprises a workbench, a liquid adding unit, a pump valve pipeline unit and an ultrasonic device, wherein the liquid adding unit, the pump valve pipeline unit and the ultrasonic device are all arranged on the workbench;
the liquid adding unit comprises a three-dimensional mechanical arm and a liquid adding needle tube, and the liquid adding needle tube is arranged on the three-dimensional mechanical arm;
the pump valve pipeline unit comprises a plurality of pump valve integrated machines, and each pump valve integrated machine is integrated with a distribution valve and an injection pump; the digestion liquid reagent bottle is connected with an inlet channel of the distribution valve, and the liquid adding needle tube is connected with an outlet channel of the distribution valve;
the ultrasonic device comprises an ultrasonic pool and a test tube rack, wherein at least one test tube is arranged in the test tube rack, the test tube rack is placed in the ultrasonic pool, and the test tube rack is positioned below the liquid adding needle tube.
Preferably, the sample pretreatment platform with the ultrasonic extraction function further comprises an electric control system; the electric control system comprises a PC upper computer, a PLC lower computer, a first motor driver, a second motor driver, a first motor and a second motor;
the PLC lower computer is respectively connected with the PC upper computer, the first motor driver and the second motor driver; the first motor driver is connected with the first motor, and the first motor is used for controlling the pump-valve integrated machine; the second motor driver is connected with the second motor, and the second motor is used for controlling the three-dimensional mechanical arm.
Preferably, the sample pretreatment platform with the ultrasonic extraction function further comprises a display screen; the display screen is connected with the PC upper computer and used for displaying the running state of the sample pretreatment platform.
Preferably, the ultrasonic pool comprises an ultrasonic generator, an ultrasonic transducer and a water tank; the ultrasonic generator is electrically connected with the ultrasonic transducer.
Preferably, the sample pretreatment platform with the ultrasonic extraction function further comprises a cleaning solution bottle; the cleaning solution bottle is connected with the inlet channel of the distribution valve.
Preferably, the number of the pump-valve integrated machines is n, the three-dimensional mechanical arm is provided with n liquid adding needle tubes, and the test tubes are n x m test tube sequences.
Preferably, the first motor comprises a dispensing valve motor for driving the dispensing valve and a syringe pump motor for driving the syringe pump; the port of the injection pump is connected with the valve of the distribution valve.
One or more technical solutions provided in the embodiments of the present application have at least the following technical effects or advantages:
according to the invention, the organic components contained in the additive substances are dissolved in the solvent as completely as possible by an ultrasonic device, and ultrasonic extraction is carried out to obtain the multi-component mixed extract, so that the sample can be comprehensively analyzed. The sample pretreatment platform provided by the invention basically realizes full-automatic treatment through the liquid adding unit and the pump valve pipeline unit, can flexibly set the type and the capacity of the digestion liquid reagent, has more various and intelligent dilution modes, and meets the application requirements of experiment in multiple directions.
Drawings
In order to more clearly illustrate the technical solution of the present embodiment, the drawings needed to be used in the description of the embodiment will be briefly introduced below, and it is obvious that the drawings in the following description are one embodiment of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on the drawings without creative efforts.
Fig. 1 is a schematic structural diagram of a sample pretreatment platform with an ultrasonic extraction function according to an embodiment of the present invention;
fig. 2 is a working schematic diagram of a sample pretreatment platform with an ultrasonic extraction function according to an embodiment of the present invention;
fig. 3 is a control block diagram of an electric control system in the sample pretreatment platform with the ultrasonic extraction function according to the embodiment of the present invention.
Wherein, the device comprises a liquid adding needle tube 1, a three-dimensional mechanical arm 2, a pump-valve integrated machine 3, a digestion solution reagent bottle 4, an ultrasonic pool 5, a test tube stand 6, a display screen 7, a PC upper computer 8, a workbench 9, a cleaning solution bottle 10 and a waste solution barrel 11;
3-1-syringe pump, 3-2-distribution valve.
Detailed Description
In order to better understand the technical solution, the technical solution will be described in detail with reference to the drawings and the specific embodiments.
The invention provides a sample pretreatment platform with an ultrasonic extraction function, as shown in fig. 1 and 2, comprising: workstation 9, liquid feeding unit, pump valve pipeline unit, ultrasonic device, electrical system, the liquid feeding unit pump valve pipeline unit the ultrasonic device all sets up on the workstation 9.
The liquid adding unit comprises a three-dimensional mechanical arm 2 and a liquid adding needle tube 1, wherein the liquid adding needle tube 1 is installed on the three-dimensional mechanical arm 2. The pump valve pipeline unit comprises a plurality of pump valve integrated machines 3, and each pump valve integrated machine 3 is integrated with a distribution valve 3-2 and an injection pump 3-1; the digestion liquid reagent bottle 4 is connected with the inlet channel of the distribution valve 3-2, and the liquid adding needle tube 1 is connected with the outlet channel of the distribution valve 3-2. The ultrasonic device comprises an ultrasonic pool 5 and a test tube rack 6, wherein at least one test tube is arranged in the test tube rack 6, the test tube rack 6 is placed in the ultrasonic pool 5, and the test tube rack 6 is positioned below the liquid adding needle tube 1.
As shown in fig. 1, a plurality of the pump-valve integrated machines 3 are arranged in a left box of the sample pretreatment platform; the upper part of the right side of the sample pretreatment platform is provided with the three-dimensional mechanical arm 2 controlled by a second motor, a plurality of liquid adding needle tubes 1 are arranged on the three-dimensional mechanical arm 2 in rows, the liquid adding needle tubes 1 are connected with the pump-valve integrated machine 3 through pipelines, and the number of the liquid adding needle tubes 1 is the same as that of the pump-valve integrated machine 3; the two ultrasonic pools 5 are arranged at the lower part of the right side of the sample pretreatment platform side by side, and n x m test tube sequences are arranged in the ultrasonic pools 5; a PC upper computer 8 and a display screen 7 are arranged on the outer side of the box body of the sample pretreatment platform, the PC upper computer 8 and the display screen 7 form a microcomputer together, and the functions of operation and state display are achieved on the sample pretreatment platform; be provided with waste liquid bucket 11, washing liquid bottle 10, a plurality of below the workstation digest reagent bottle 4, waste liquid bucket 11 the washing liquid bottle 11 digest reagent bottle 4 respectively through the pipeline with pump valve all-in-one 3 is connected.
For example, the three-dimensional mechanical arm 2 is provided with six liquid adding needle tubes 1 arranged in a row, and each time the sample pretreatment platform runs, the six liquid adding needle tubes 1 work simultaneously.
For example, the pump-valve pipeline unit adopts six high-precision integrated pump-valve machines 3, each distribution valve 3-2 has 12 channels, and the 12 channels can be classified into 4 types according to functions: the digestion liquid inlet channel, the digestion liquid outlet channel, the cleaning liquid inlet channel and the cleaning liquid outlet channel can be defined through PLC programming. Defining a certain channel of the distribution valve 3-2 as an inlet channel, which is equivalent to defining a certain digestion solution, the injection pump 3-1 extracts a certain amount of digestion solution, then defining a certain channel of the distribution valve 3-2 as an outlet channel, and the injection pump 3-1 transmits the digestion solution to the liquid adding needle tube 1 through a pipeline system, and then the digestion solution is added into a test tube containing a sample through the three-dimensional mechanical arm 2.
The invention integrates the distribution valve 3-2 and the injection pump 3-1, the distribution valve 3-2 is driven by a distribution valve motor, the injection pump 3-1 is driven by an injection pump motor, and the distribution valve 3-2 and the injection pump 3-1 are assembled together; the plurality of channels of the distribution valve 3-2 are connected with different digestion solution reagent bottles 4, cleaning solution bottles and liquid adding needle tubes 1 through pipelines, and the injection pump 3-1 draws or pushes out the solution and passes through the distribution valve 3-2, namely, the port of the injection pump 3-1 is directly connected with the valve of the distribution valve 3-2.
The work flow of the pump-valve all-in-one machine 3 can be expressed as: defining the dispensing valve inlet-defining the syringe pump intake stroke (draw dose of solution) -defining the dispensing valve outlet-the syringe pump push-out stroke.
The ultrasonic pool 5 comprises an ultrasonic generator, an ultrasonic transducer and a water tank; the ultrasonic generator is electrically connected with the ultrasonic transducer. And placing the test tube rack 6 in the ultrasonic pool 5, and after the device finishes a digestion scheme, enabling the ultrasonic device to enter a working mode, and carrying out ultrasonic water bath heating extraction treatment on the sample.
The positions of the ultrasonic pool 5 and the test tube rack 6 can refer to fig. 2, and the test tube rack 6 is placed in the ultrasonic pool 5, but the bottom of the test tube in the test tube rack 6 is ensured not to contact with the bottom of the water tank. When the ultrasonic pool 5 is filled with a certain amount of water and the power is switched on, the ultrasonic generator and the ultrasonic transducer can convert the alternating current with the set frequency into the alternating current with the ultrasonic frequency to generate oscillation, and the solution is heated and extracted in a water bath.
The electric control system enables the sample pretreatment platform to be fully automatic and high in efficiency. According to the invention, the PC upper computer 8, the PLC lower computer and the motor driver control the motor, so that the pump valve integrated machine 3 and the three-dimensional mechanical arm 2 are controlled to act, different digestion schemes are realized through the pump valve integrated machine 3, and a plurality of liquid adding needle tubes 1 are driven by the three-dimensional mechanical arm 2 to work at a plurality of point positions simultaneously, so that the working efficiency is increased. One digestion type can contain 5 to 6 digestion liquids with settable dosages, so that the configuration scheme is diversified and the pretreatment system with multiple schemes is satisfied. The number of the digestion solution reagent bottles 4 is not limited, and the number of digestion solutions contained in one digestion type (protocol) and the dosage thereof may be changed. This change can be implemented only by means of a PLC program. The pump-valve integrated machine 3 extracts the required dosage of each digestion solution, the dosage is compiled into an instruction during program design, and the PLC lower computer is communicated with the pump-valve integrated machine 3 and transmits the instruction to the pump-valve integrated machine 3, so that the injection pump 3-1 extracts the required dosage.
The control method of the invention is as shown in fig. 3, the pump-valve integrated machine 3 is controlled by the first motor (comprising a distribution valve motor and an injection pump motor), the three-dimensional mechanical arm 2 is controlled by the second motor (comprising three motors of an X axis, a Y axis and a Z axis) to drive the liquid adding needle tube 1 to move, and the digestion solution reagent bottle 4, the pump-valve integrated machine 3 and the liquid adding needle tube 1 are communicated through a pipeline. The operation of the first motor and the second motor is controlled by the PC upper computer 8, the PLC lower computer and the motor driver, 5-6 reagents with different dosages can be selected by controlling the operation of the motors and added into a set test tube sequence, so that the functions of sample transfer, sample distribution, multiple reagent addition and cleaning are realized, and the configuration types are more diversified.
The injection pump 3-1 and the distribution valve 3-2 are arranged on one side of the pump-valve integrated machine shell and are respectively controlled by the injection pump motor and the distribution valve motor, one end of a circuit board in the pump-valve integrated machine is provided with an interface of an organism, and the interface is communicated with external equipment (such as a PLC (programmable logic controller) lower computer), so that the injection pump motor and the distribution valve motor are driven by the PLC lower computer, and the pump-valve integrated machine 3 works. The design is more integrated, and the electric control system can be optimized.
After the device finishes the digestion scheme, the ultrasonic device can enter a working mode under the condition of meeting the working environment of the ultrasonic extraction device, and ultrasonic water bath heating extraction treatment is carried out on the sample to prepare for further treatment.
The embodiment provides a sample pretreatment platform with an ultrasonic extraction function, which can be used for carrying out ultrasonic extraction on a pretreated sample and layering different substances in a solution, so that the original sample can be comprehensively analyzed. The sample pretreatment platform provided by the invention basically realizes full automation, the types and the capacities of reagents can be flexibly set, the dilution mode is more diversified and intelligent, and the multidirectional application requirements of experiments are met.
Finally, it should be noted that the above embodiments are only for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to examples, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, which should be covered by the claims of the present invention.
Claims (5)
1. A sample pretreatment platform with an ultrasonic extraction function is characterized by comprising: the ultrasonic cleaning device comprises a workbench, a liquid adding unit, a pump valve pipeline unit, an ultrasonic device, an electric control system and a cleaning liquid bottle, wherein the liquid adding unit, the pump valve pipeline unit and the ultrasonic device are all arranged on the workbench;
the liquid adding unit comprises a three-dimensional mechanical arm and a liquid adding needle tube, and the liquid adding needle tube is arranged on the three-dimensional mechanical arm;
the pump valve pipeline unit comprises a plurality of pump valve integrated machines, and each pump valve integrated machine is integrated with a distribution valve and an injection pump; the digestion liquid reagent bottle is connected with an inlet channel of the distribution valve, and the liquid adding needle tube is connected with an outlet channel of the distribution valve; the cleaning liquid bottle is connected with the inlet channel of the distribution valve;
the ultrasonic device comprises an ultrasonic pool and a test tube rack, wherein at least one test tube is arranged in the test tube rack, the test tube rack is placed in the ultrasonic pool, and the test tube rack is positioned below the liquid adding needle tube;
the electric control system comprises a PC upper computer, a PLC lower computer, a first motor driver, a second motor driver, a first motor and a second motor;
the PLC lower computer is respectively connected with the PC upper computer, the first motor driver and the second motor driver; the first motor driver is connected with the first motor, and the first motor is used for controlling the pump-valve integrated machine; the second motor driver is connected with the second motor, and the second motor is used for controlling the three-dimensional mechanical arm.
2. The sample pretreatment platform with ultrasonic extraction function of claim 1, further comprising a display screen; the display screen is connected with the PC upper computer and used for displaying the running state of the sample pretreatment platform.
3. The sample pretreatment platform with the ultrasonic extraction function as claimed in claim 1, wherein the ultrasonic pool comprises an ultrasonic generator, an ultrasonic transducer and a water tank; the ultrasonic generator is electrically connected with the ultrasonic transducer.
4. The sample pretreatment platform with the ultrasonic extraction function according to claim 1, wherein the number of the pump-valve integrated machines is n, the three-dimensional mechanical arm is provided with n liquid adding needle tubes, and the test tubes are n x m test tube sequences.
5. The sample pretreatment platform with ultrasonic extraction function of claim 1, wherein the first motor comprises a distribution valve motor and a syringe pump motor, the distribution valve motor is used for driving the distribution valve, and the syringe pump motor is used for driving the syringe pump; the port of the injection pump is connected with the valve of the distribution valve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810430579.3A CN108760420B (en) | 2018-05-08 | 2018-05-08 | Sample pretreatment platform with ultrasonic extraction function |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810430579.3A CN108760420B (en) | 2018-05-08 | 2018-05-08 | Sample pretreatment platform with ultrasonic extraction function |
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| CN108760420A CN108760420A (en) | 2018-11-06 |
| CN108760420B true CN108760420B (en) | 2020-12-01 |
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| CN201810430579.3A Expired - Fee Related CN108760420B (en) | 2018-05-08 | 2018-05-08 | Sample pretreatment platform with ultrasonic extraction function |
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| CN114778266B (en) * | 2022-04-28 | 2024-05-07 | 中国第一重型机械股份公司 | High-temperature melting digestion method and device for chemical analysis sample |
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| EP1442294A4 (en) * | 2001-11-16 | 2005-01-19 | Waters Investments Ltd | Parallel concentration, desalting and deposition onto maldi targets |
| CN201579027U (en) * | 2009-12-18 | 2010-09-15 | 孔兵 | Full-automatic liquid operating device |
| CN205483729U (en) * | 2016-02-24 | 2016-08-17 | 中国水产科学研究院长江水产研究所 | Drug residue extraction element in fish tissue |
| CN106199032B (en) * | 2016-06-30 | 2018-05-08 | 南京润泽流体控制设备有限公司 | A kind of sample injection method of no cross contamination |
| CN206362811U (en) * | 2016-12-07 | 2017-07-28 | 上海通微分析技术有限公司 | A kind of automatic sampler for quantitative capillary tube electrophoresis apparatus |
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