CN108744013B - 纳米银-葛根纤维敷料的制备方法及应用 - Google Patents
纳米银-葛根纤维敷料的制备方法及应用 Download PDFInfo
- Publication number
- CN108744013B CN108744013B CN201810321841.0A CN201810321841A CN108744013B CN 108744013 B CN108744013 B CN 108744013B CN 201810321841 A CN201810321841 A CN 201810321841A CN 108744013 B CN108744013 B CN 108744013B
- Authority
- CN
- China
- Prior art keywords
- solution
- radix puerariae
- silver
- fiber
- nano silver
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000000835 fiber Substances 0.000 title claims abstract description 65
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 239000002244 precipitate Substances 0.000 claims abstract description 27
- 235000010575 Pueraria lobata Nutrition 0.000 claims abstract description 24
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims abstract description 22
- 238000010041 electrostatic spinning Methods 0.000 claims abstract description 18
- 239000000203 mixture Substances 0.000 claims abstract description 15
- 244000046146 Pueraria lobata Species 0.000 claims abstract description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000002033 PVDF binder Substances 0.000 claims abstract description 11
- 235000014113 dietary fatty acids Nutrition 0.000 claims abstract description 11
- 239000000194 fatty acid Substances 0.000 claims abstract description 11
- 229930195729 fatty acid Natural products 0.000 claims abstract description 11
- 150000004665 fatty acids Chemical class 0.000 claims abstract description 11
- 229920001522 polyglycol ester Polymers 0.000 claims abstract description 11
- 229920002981 polyvinylidene fluoride Polymers 0.000 claims abstract description 11
- 238000009987 spinning Methods 0.000 claims abstract description 11
- 239000004094 surface-active agent Substances 0.000 claims abstract description 11
- 230000010355 oscillation Effects 0.000 claims abstract description 10
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 9
- 229910000367 silver sulfate Inorganic materials 0.000 claims abstract description 5
- 238000006386 neutralization reaction Methods 0.000 claims abstract description 3
- 239000000243 solution Substances 0.000 claims description 62
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 241000219781 Pueraria montana var. lobata Species 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 10
- 238000005406 washing Methods 0.000 claims description 10
- 239000000843 powder Substances 0.000 claims description 9
- 239000011259 mixed solution Substances 0.000 claims description 7
- 230000007935 neutral effect Effects 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 239000008399 tap water Substances 0.000 claims description 5
- 235000020679 tap water Nutrition 0.000 claims description 5
- 229910020489 SiO3 Inorganic materials 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 3
- CSNNHWWHGAXBCP-UHFFFAOYSA-L magnesium sulphate Substances [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 3
- 229910052911 sodium silicate Inorganic materials 0.000 claims description 3
- 239000000126 substance Substances 0.000 abstract description 11
- 229930003935 flavonoid Natural products 0.000 abstract description 10
- 150000002215 flavonoids Chemical class 0.000 abstract description 10
- 235000017173 flavonoids Nutrition 0.000 abstract description 10
- 230000023597 hemostasis Effects 0.000 abstract description 8
- 230000035699 permeability Effects 0.000 abstract description 8
- RXUWDKBZZLIASQ-UHFFFAOYSA-N Puerarin Natural products OCC1OC(Oc2c(O)cc(O)c3C(=O)C(=COc23)c4ccc(O)cc4)C(O)C(O)C1O RXUWDKBZZLIASQ-UHFFFAOYSA-N 0.000 abstract description 4
- HKEAFJYKMMKDOR-VPRICQMDSA-N puerarin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1C1=C(O)C=CC(C2=O)=C1OC=C2C1=CC=C(O)C=C1 HKEAFJYKMMKDOR-VPRICQMDSA-N 0.000 abstract description 4
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 2
- 239000000463 material Substances 0.000 description 13
- 230000002439 hemostatic effect Effects 0.000 description 9
- 210000004369 blood Anatomy 0.000 description 8
- 239000008280 blood Substances 0.000 description 8
- 229920000742 Cotton Polymers 0.000 description 5
- 239000004744 fabric Substances 0.000 description 5
- 208000032843 Hemorrhage Diseases 0.000 description 4
- 208000027418 Wounds and injury Diseases 0.000 description 4
- 210000001367 artery Anatomy 0.000 description 4
- 241000283973 Oryctolagus cuniculus Species 0.000 description 3
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 238000005520 cutting process Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 229930003944 flavone Natural products 0.000 description 3
- 150000002212 flavone derivatives Chemical class 0.000 description 3
- 235000011949 flavones Nutrition 0.000 description 3
- 238000004128 high performance liquid chromatography Methods 0.000 description 3
- 208000014674 injury Diseases 0.000 description 3
- 239000011734 sodium Substances 0.000 description 3
- 238000001179 sorption measurement Methods 0.000 description 3
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 3
- 206010019233 Headaches Diseases 0.000 description 2
- 230000000740 bleeding effect Effects 0.000 description 2
- 230000017531 blood circulation Effects 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 231100000869 headache Toxicity 0.000 description 2
- 238000013168 hemostasis test Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000002107 myocardial effect Effects 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 210000003462 vein Anatomy 0.000 description 2
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 206010002383 Angina Pectoris Diseases 0.000 description 1
- 208000003322 Coinfection Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 206010029216 Nervousness Diseases 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- QGMRQYFBGABWDR-UHFFFAOYSA-M Pentobarbital sodium Chemical compound [Na+].CCCC(C)C1(CC)C(=O)NC(=O)[N-]C1=O QGMRQYFBGABWDR-UHFFFAOYSA-M 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 208000005392 Spasm Diseases 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 208000002173 dizziness Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004089 microcirculation Effects 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000036284 oxygen consumption Effects 0.000 description 1
- 230000036407 pain Effects 0.000 description 1
- 229960002275 pentobarbital sodium Drugs 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 235000019795 sodium metasilicate Nutrition 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/40—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing ingredients of undetermined constitution or reaction products thereof, e.g. plant or animal extracts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/18—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing inorganic materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/24—Macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/26—Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/425—Porous materials, e.g. foams or sponges
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/46—Deodorants or malodour counteractants, e.g. to inhibit the formation of ammonia or bacteria
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0015—Electro-spinning characterised by the initial state of the material
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/10—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing inorganic materials
- A61L2300/102—Metals or metal compounds, e.g. salts such as bicarbonates, carbonates, oxides, zeolites, silicates
- A61L2300/104—Silver, e.g. silver sulfadiazine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/30—Compounds of undetermined constitution extracted from natural sources, e.g. Aloe Vera
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/418—Agents promoting blood coagulation, blood-clotting agents, embolising agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/04—Materials for stopping bleeding
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Epidemiology (AREA)
- Hematology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Botany (AREA)
- Zoology (AREA)
- Dispersion Chemistry (AREA)
- Mechanical Engineering (AREA)
- Textile Engineering (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Artificial Filaments (AREA)
Abstract
本发明提供的纳米银‑葛根纤维敷料的制备方法,包括以下步骤:利用Ag2SO4溶液对葛根纤维进行脱胶处理,并生成银‑葛根纤维沉淀物;对银‑葛根纤维沉淀物表面进行中性化处理;用75%的乙醇溶液溶解处理后的银‑葛根纤维沉淀物,超声震荡直至溶液变为稳定的黄色溶液,形成葛根纤维‑纳米银混合剂;在葛根纤维‑纳米银混合剂中加入聚偏二氟乙烯、二甲亚砜、脂肪酸聚乙二醇酯、表面活性剂,超声震荡至均匀,制成静电纺丝液;使用静电纺丝溶液纺丝,制成纳米银‑葛根纤维敷料。本发明的制备方法,使葛根中的类黄酮物质成份大比例地保留在葛根纤维素中,使制成的敷料止血迅速、透气性好、抗菌能力强。
Description
技术领域
本发明属于生物制造技术领域,特别涉及纳米银-葛根纤维敷料的制备方法及应用。
背景技术
由于失血是战伤伤员死亡最重要的原因之一,因此多功能性高端快速止血敷料是在战场和日常各种创伤发生情况下进行急救的重要工具。战伤失血通常分为体内出血和体外出血,体内出血大部分是因为脏器破裂,体外出血基本是由于动静脉损伤。如果能及时有效地止血,对挽救伤员生命,稳定伤情,为后续治疗创造条件十分重要。敷料作为止血材料,是指盖在伤口上、有保护作用的覆盖物,可以协助控制出血,防止感染并吸收分泌物,止血敷料对于及时止血有着重要的意义。
葛根,为豆科植物野葛,是中国南方一些省区的一种常食蔬菜,其味甘凉可口,常作煲汤之用。现代医学表明:葛根中含有大量的葛根素,能增加冠状动脉血流量、降低血管阻力、调节血液循环、降低心肌耗氧等,是一种较为常见的心肌保护物质,能解痉止痛,防治高血压、头晕头痛、冠心病、心绞痛、神经性头痛等。但事实上,除葛根素外,葛根中因品种不同至少还含有12~18%的类黄酮物质,黄酮类物质具有扩张外周血管、改善微循环,祛腐生肌,解热收敛的作用,是上等的止血材料,再加上葛根纤维经特殊处理后,具有组织疏松、吸附力强等特性,二者结合其实具有成为高端快速止血材料的可能,但国内对此方面却鲜有研究。
发明内容
本发明的目的是为解决以上问题,本发明提供纳米银-葛根纤维敷料的制备方法及应用。本发明的技术方案是通过以下实现的。
根据本发明的一个方面提供纳米银-葛根纤维敷料的制备方法,包括以下步骤:
(1)利用Ag2SO4溶液对葛根纤维进行脱胶处理,并生成银-葛根纤维沉淀物;
(2)对银-葛根纤维沉淀物表面进行中性化处理;
(3)用75%的乙醇溶液溶解处理后的银-葛根纤维沉淀物,超声震荡直至溶液变为稳定的黄色溶液,形成葛根纤维-纳米银混合剂;
(4)在葛根纤维-纳米银混合剂中加入聚偏二氟乙烯、二甲亚砜、脂肪酸聚乙二醇酯、表面活性剂,超声震荡至均匀,制成静电纺丝液;
(5)使用静电纺丝溶液纺丝,生成纳米银-葛根纤维敷料。
其中,步骤(1)包括:
使用浓度为1~1.5mL/L的H2SO4溶解葛根粉末,置于40℃的水浴锅中30~60min,用自来水冲洗至中性;再加入由浓度为30mL/L的Na2SiO3溶液、质量浓度为12.75g/L的MgSO4溶液、质量浓度为40g/mL的NaOH溶液和H2O2混合形成的混合液,并置于85℃的水浴锅中60~80min,搅拌;然后加入浓度为0.8~1.6g/L的Ag2SO4溶液将溶液调节至中性;过滤得到银-葛根纤维沉淀物。
其中,步骤(1)包括:H2SO4和葛根粉末的浴比为5:1,混合液和葛根粉末的浴比为4:1。
其中,步骤(1)包括:葛根为250g,H2SO4为1L,Na2SiO3溶液为30mL,MgSO4溶液为100mL,NaOH为210mL,H2O2为660mL,Ag2SO4溶液为400mL。
其中,步骤(4)包括:葛根纤维-纳米银混合剂:聚偏二氟乙烯:二甲亚砜:脂肪酸聚乙二醇酯:表面活性剂=270~290:35~45:12~15:1~1.5:1~1.5,超声震荡时间为30~50min。
其中,步骤(5)包括:静电纺丝电压为6~8kV,针头与接收板之间的距离为10~12cm,推进泵的推进速度为0.30~0.40mm/h,纺丝环境温度为30~35℃。
其中,步骤(2)包括:用温水冲洗银-葛根纤维沉淀物,使银-葛根纤维沉淀物的表面为中性。
其中,步骤(3)包括:超声震荡60~90min。
根据本发明的另一方面提供该制备方法制备的纳米银-葛根纤维敷料在止血方面的应用。
本发明具有以下有益效果:
1.本发明的制备方法,使葛根中类黄酮物质大比例地保留在葛根纤维素中,因此使制成的敷料具有良好的止血功能。
2.本方法制备的敷料主要成份为天然葛根纤维,对血液的吸收能力和材料自身透气性均优于普通的止血功能材料。
3.本发明将葛根纤维、纳米银和高分子聚合物通过静电纺丝技术结合,使形成的敷料具有较大的比表面积和微孔结构,一方面能够引导纤维迅速深入至伤口深处,修复受伤组织,另一方面使材料具备良好的抗菌能力,能够避免二次感染。
具体实施方式
下面将更详细地描述本公开的示例性实施方式。虽然本说明书中显示了本公开的示例性实施方式,然而应当理解,可以以各种形式实现本公开而不应被这里阐述的实施方式所限制。相反,提供这些实施方式是为了能够更透彻地理解本公开,并且能够将本公开的范围完整的传达给本领域的技术人员。
实施例1纳米银-葛根纤维敷料X1的制备方法及应用
包括以下步骤:
使用1L浓度为1mL/L的H2SO4溶解250g葛根粉末,置于40℃的水浴锅中45min,用自来水冲洗至溶液的PH至为7;再加入由30mL浓度为30mL/L的Na2SiO3溶液、100mL质量浓度为12.75g/L的MgSO4溶液、210mL质量浓度为40g/mL的NaOH溶液和660mL的H2O2混合形成的混合液,并置于85℃的水浴锅中80min,搅拌10min;然后加入400mL浓度为1.5g/L的Ag2SO4溶液使得溶液的PH至为7;。用布氏漏斗过滤得到银-葛根纤维沉淀物;使用30℃的温水冲洗银-葛根纤维沉淀物,至银-葛根纤维沉淀物的表面的PH值为7.2;用75%的乙醇溶液溶解处理后的银-葛根纤维沉淀物,超声震荡90min至溶液变为稳定的黄色溶液,形成葛根纤维-纳米银混合剂;在葛根纤维-纳米银混合剂中加入聚偏二氟乙烯、二甲亚砜、脂肪酸聚乙二醇酯、表面活性剂,超声震荡30min至均匀,制成静电纺丝液,其中,葛根纤维-纳米银混合剂:聚偏二氟乙烯:二甲亚砜:脂肪酸聚乙二醇酯:表面活性剂=270:40:12:1.5:1.5;使用静电纺丝溶液纺丝,制成纳米银-葛根纤维敷料X1,其中静电纺丝电压为6kV,针头与接收板之间的距离为10cm,推进泵的推进速度为0.40mm/h,纺丝环境温度为30℃。
使用高效液相色谱法(HPLC)对纳米银-葛根纤维敷料X1的类黄酮物质含量进行测定,经检测X1中类黄酮物质含量为15g,因此实施例1的制备方法黄酮的提取率为6%。
实施例2纳米银-葛根纤维敷料X2的制备方法及应用
包括以下步骤:
使用0.4L浓度为1mL/L的H2SO4溶解100g葛根粉末,置于40℃的水浴锅中45min,用自来水冲洗至溶液的PH至为7.3;再加入由12mL浓度为30mL/L的Na2SiO3溶液、40mL质量浓度为12.75g/L的MgSO4溶液、84mL质量浓度为40g/mL的NaOH溶液和264mL的H2O2混合形成的混合液,并置于85℃的水浴锅中80min,搅拌5min;然后加入160mL浓度为1.5g/L的Ag2SO4溶液使得溶液的PH至为7.4;。用布氏漏斗过滤得到银-葛根纤维沉淀物;使用50℃的温水冲洗银-葛根纤维沉淀物,至银-葛根纤维沉淀物的表面的PH值为7.5;用75%的乙醇溶液溶解处理后的银-葛根纤维沉淀物,超声震荡60min至溶液变为稳定的黄色溶液,形成葛根纤维-纳米银混合剂;在葛根纤维-纳米银混合剂中加入聚偏二氟乙烯、二甲亚砜、脂肪酸聚乙二醇酯、表面活性剂,超声震荡35min至均匀,制成静电纺丝液,其中,葛根纤维-纳米银混合剂:聚偏二氟乙烯:二甲亚砜:脂肪酸聚乙二醇酯:表面活性剂=290:45:15:1.2:1.5;使用静电纺丝溶液纺丝,制成纳米银-葛根纤维敷料X3,其中静电纺丝电压为8kV,针头与接收板之间的距离为10cm,推进泵的推进速度为0.30mm/h,纺丝环境温度为35℃。
使用高效液相色谱法(HPLC)对纳米银-葛根纤维敷料X2的类黄酮物质含量进行测定,经检测X2中类黄酮物质含量为6.2g,因此实施例2的制备方法黄酮的提取率为6.2%。
实施例3纳米银-葛根纤维敷料X3的制备方法及应用
使用0.1L浓度为1mL/L的H2SO4溶解25g葛根粉末,置于40℃的水浴锅中45min,用自来水冲洗至溶液的PH至为6.8;再加入由3mL浓度为30mL/L的Na2SiO3溶液、10mL质量浓度为12.75g/L的MgSO4溶液、21mL质量浓度为40g/mL的NaOH溶液和66mL的H2O2混合形成的混合液,并置于85℃的水浴锅中80min,搅拌7min;然后加入40mL浓度为1.5g/L的Ag2SO4溶液使得溶液的PH至为7.1;。用布氏漏斗过滤得到银-葛根纤维沉淀物;使用50℃的温水冲洗银-葛根纤维沉淀物,至银-葛根纤维沉淀物的表面的PH值为7.1;用75%的乙醇溶液溶解处理后的银-葛根纤维沉淀物,超声震荡80min至溶液变为稳定的黄色溶液,形成葛根纤维-纳米银混合剂;在葛根纤维-纳米银混合剂中加入聚偏二氟乙烯、二甲亚砜、脂肪酸聚乙二醇酯、表面活性剂,超声震荡45min至均匀,制成静电纺丝液,其中,葛根纤维-纳米银混合剂:聚偏二氟乙烯:二甲亚砜:脂肪酸聚乙二醇酯:表面活性剂=270:35:14:1:1;使用静电纺丝溶液纺丝,制成纳米银-葛根纤维敷料X2,其中静电纺丝电压为8kV,针头与接收板之间的距离为12cm,推进泵的推进速度为0.40mm/h,纺丝环境温度为32℃。
使用高效液相色谱法(HPLC)对纳米银-葛根纤维敷料X3的类黄酮物质含量进行测定,经检测X2中类黄酮物质含量为1.38g,因此实施例2的制备方法黄酮的提取率为5.52%。
实验例1血液吸收实验
①.实验步骤:称取20ml血液置于烧杯中,分别裁切出1cm*1cm尺寸的纳米银-葛根纤维敷料X1~X3投入烧杯中,其中纳米银-葛根纤维敷料X1~X3的厚度均为0.3毫米,记录吸附全部血液所需时间,如不能完成吸附,记录最大吸附时间。
②.实验结果:
纳米银-葛根纤维敷料X1~X3均顺利完成吸附,其中纳米银-葛根纤维敷料X1所需时间为8s,纳米银-葛根纤维敷料X2所需时间为10s,蜂胶-纳米银敷料X3所需时间为10s。
实验例2止血实验
①.实验步骤:选取30只体重为6.0~6.3kg的比利时长耳兔,随机分为3组,每组10只,进行止血测试实验。按40mg/kg剂量静脉缓慢注射戊巴比妥钠溶液麻醉后,将其中央耳动脉区域备皮、消毒,沿耳动脉方向切开皮肤,钝性分离出耳动脉、静脉和神经,再用手术刀横向切断动脉,待血液涌出后立即用1层尺寸为1*1cm的待测试材料并使用推拉力计施加3N的压力,每隔10s观察止血情况,直至最终完全止血后记录止血时间,计算每组所用平均时间。其中每组使用一种实施例1~3中的纳米银-葛根纤维敷料作为待测试材料。
②.实验结果:
纳米银-葛根纤维X1~X3的平均止血时间分别为100s,105s和111s。
实验例3
实验材料:厚度均为0.620mm的纳米银-葛根纤维敷料X1~X3和纯棉织物Y,其中纯棉织物Y的规格见表1所示。
实验步骤:使用GELLOWEN透气性测试仪依次对敷料X1~X3和纯棉织物Y进行透气度测试,每种材料测试10次,取均值即为该种材料的透气度。
实验结果:经检测纳米银-葛根纤维敷料X1~X3的平均透气度分别为38.7L/min、32.9L/min和35.7L/min,而纯棉织物Y的平均透气度为15.1L/min。
综上所述,本发明制备的纳米银-葛根纤维敷料除具有优异的吸收和止血能力外,其透气性更加优于相同厚度的纯棉织物。
以上所述,仅为本发明较佳的具体实施方式,但本发明的保护范围并不局限于此,任何熟悉本技术领域的技术人员在本发明揭露的技术范围内,可轻易想到的变化或替换,都应涵盖在本发明的保护范围之内。因此,本发明的保护范围应以所述权利要求的保护范围为准。
Claims (8)
1.纳米银-葛根纤维敷料的制备方法,其特征在于,包括以下步骤:
(1)利用Ag2SO4溶液对葛根纤维进行脱胶处理,并生成银-葛根纤维沉淀物;
(2)对银-葛根纤维沉淀物表面进行中性化处理;
(3)用75%的乙醇溶液溶解处理后的银-葛根纤维沉淀物,超声震荡直至溶液变为稳定的黄色溶液,形成葛根纤维-纳米银混合剂;
(4)在葛根纤维-纳米银混合剂中加入聚偏二氟乙烯、二甲亚砜、脂肪酸聚乙二醇酯、表面活性剂,超声震荡至均匀,制成静电纺丝液;
(5)使用静电纺丝溶液纺丝,制成纳米银-葛根纤维敷料。
2.如权利要求1所述的制备方法,其特征在于,所述步骤(1)包括:
使用浓度为1~1.5mL/L的H2SO4溶解葛根粉末,置于40℃的水浴锅中30~60min,用自来水冲洗至中性;再加入由浓度为30mL/L的Na2SiO3溶液、质量浓度为12.75g/L的MgSO4溶液、质量浓度为40g/mL的NaOH溶液和H2O2混合形成的混合液,并置于85℃的水浴锅中60~80min,搅拌;然后加入浓度为0.8~1.6g/L的Ag2SO4溶液将溶液调节至中性;过滤得到银-葛根纤维沉淀物。
3.如权利要求2所述的制备方法,其特征在于,所述步骤(1)包括:
其中,H2SO4和葛根粉末的浴比为5:1,混合液和葛根粉末的浴比为4:1。
4.如权利要求2所述的制备方法,其特征在于,所述步骤(1)包括:
其中,葛根为250g,H2SO4为1L,Na2SiO3溶液为30mL,MgSO4溶液为100mL,NaOH为210mL,H2O2为660mL,Ag2SO4溶液为400mL。
5.如权利要求1所述的制备方法,其特征在于,所述步骤(4)包括:
葛根纤维-纳米银混合剂:聚偏二氟乙烯:二甲亚砜:脂肪酸聚乙二醇酯:表面活性剂=270~290:35~45:12~15:1~1.5:1~1.5,超声震荡时间为30~50min。
6.如权利要求1所述的制备方法,其特征在于,所述步骤(5)包括:
静电纺丝电压为6~8kV,针头与接收板之间的距离为10~12cm,推进泵的推进速度为0.30~0.40mm/h,纺丝环境温度为30~35℃。
7.如权利要求1所述的制备方法,其特征在于,所述步骤(2)包括:
用温水冲洗银-葛根纤维沉淀物,使银-葛根纤维沉淀物的表面为中性。
8.如权利要求1所述的制备方法,其特征在于,所述步骤(3)包括:
超声震荡60~90min。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810321841.0A CN108744013B (zh) | 2018-04-11 | 2018-04-11 | 纳米银-葛根纤维敷料的制备方法及应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810321841.0A CN108744013B (zh) | 2018-04-11 | 2018-04-11 | 纳米银-葛根纤维敷料的制备方法及应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN108744013A CN108744013A (zh) | 2018-11-06 |
CN108744013B true CN108744013B (zh) | 2020-12-04 |
Family
ID=63981422
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201810321841.0A Active CN108744013B (zh) | 2018-04-11 | 2018-04-11 | 纳米银-葛根纤维敷料的制备方法及应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN108744013B (zh) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN117259772B (zh) * | 2023-08-25 | 2024-05-10 | 江苏农林职业技术学院 | 一种葛根纳米银复合水溶胶及其制备方法与应用 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101781801A (zh) * | 2010-01-29 | 2010-07-21 | 武汉科技学院 | 一种从葛根废渣中制备纺纱用葛根纤维的方法 |
CN103088630A (zh) * | 2011-10-28 | 2013-05-08 | 中国科学院化学研究所 | 一种用于促进伤口愈合的纳米纤维薄膜的制备方法 |
CN103656741A (zh) * | 2013-11-21 | 2014-03-26 | 无锡中科光远生物材料有限公司 | 一种抑菌膜 |
BR102016018498A2 (pt) * | 2016-08-12 | 2018-02-27 | Universidade Federal Do Rio Grande - Furg | Compostos bioativos integrados a nanofibras para obtenção de biomateriais para uso farmacológico |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20150104782A (ko) * | 2014-03-06 | 2015-09-16 | 임관빈 | 노근 및 칡 추출물 나노캡슐을 포함하는 아토피 개선용 직물시트 |
-
2018
- 2018-04-11 CN CN201810321841.0A patent/CN108744013B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101781801A (zh) * | 2010-01-29 | 2010-07-21 | 武汉科技学院 | 一种从葛根废渣中制备纺纱用葛根纤维的方法 |
CN103088630A (zh) * | 2011-10-28 | 2013-05-08 | 中国科学院化学研究所 | 一种用于促进伤口愈合的纳米纤维薄膜的制备方法 |
CN103656741A (zh) * | 2013-11-21 | 2014-03-26 | 无锡中科光远生物材料有限公司 | 一种抑菌膜 |
BR102016018498A2 (pt) * | 2016-08-12 | 2018-02-27 | Universidade Federal Do Rio Grande - Furg | Compostos bioativos integrados a nanofibras para obtenção de biomateriais para uso farmacológico |
Non-Patent Citations (2)
Title |
---|
Effects of puerarin on the inflammatory role of burn-related procedural pain mediated by P2X7 receptors;Jun Zhang等;《BURNS》;20131231;第39卷;第610-618页 * |
Preparation of Bimetallic Nanoparticles Using a Facile Green Synthesis Method and Their Application;Bihua Xia等;《Langmuir》;20130321;第29卷;第4901-4907页 * |
Also Published As
Publication number | Publication date |
---|---|
CN108744013A (zh) | 2018-11-06 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103550817B (zh) | 一种细菌纤维素/壳聚糖复合海绵敷料及其制备方法 | |
CN103055344B (zh) | 一种细菌纤维素/壳聚糖复合医用敷料的制备方法 | |
AU2005329351A1 (en) | The preparing method and the use of antiseptic medical dressing. | |
CN113058070B (zh) | 一种快速止血敷料及其制备方法 | |
CN101596207A (zh) | 用于迅速止血的药用组合物及制备方法 | |
CN109731121A (zh) | 一种含有介孔二氧化硅的纤维素和壳聚糖复合敷料的制备方法 | |
CN108744022A (zh) | 一种功能性可吸收止血粉及其制备方法 | |
CN113018502B (zh) | 一种医用多功能止血敷料及其制备方法 | |
CN107184961A (zh) | 一种活性细胞修复剂及其制备方法 | |
CN101927027A (zh) | 壳聚糖/羧甲基壳聚糖快速止血海绵及其制备方法 | |
CN105396170A (zh) | 一种医用手术缝合线及其制备方法 | |
CN108744013B (zh) | 纳米银-葛根纤维敷料的制备方法及应用 | |
CN105251042A (zh) | 一种医用可吸收聚乙醇酸(pga)手术缝合线及制法 | |
CN105251040A (zh) | 一种可吸收的医用抗感染缝合线及制备方法 | |
CN107496973B (zh) | 一种快速止血壳聚糖海绵垫创可贴及其制备方法 | |
CN104840749B (zh) | 一种治疗皮肤创伤而不含重金属的外用软膏及制备方法 | |
CN204158771U (zh) | 一种细菌纤维素/壳聚糖复合海绵敷料 | |
CN112813516A (zh) | 含银杏、玫瑰花、山茶花、红石榴、葛根的粘胶大生物纤维及其制备方法 | |
CN111012945A (zh) | 一种新型防水中药液体创可贴及其制备方法 | |
CN107412520A (zh) | 一种抗菌止血药膏、药物及其制备方法 | |
CN105288703A (zh) | 杀菌消炎无纺布敷料及制备方法 | |
CN106725878A (zh) | 一种新型医疗用组合包 | |
CN107126540A (zh) | 一种止血抗菌药膏、药物及其制备方法 | |
CN115721674B (zh) | 一种创口护理用组合物及其应用 | |
CN110269891A (zh) | 一种急诊科护理用快速止血贴剂及其制备方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |