CN108744013A - The preparation method and application of nano silver-pueraria lobata fiber dressing - Google Patents

The preparation method and application of nano silver-pueraria lobata fiber dressing Download PDF

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CN108744013A
CN108744013A CN201810321841.0A CN201810321841A CN108744013A CN 108744013 A CN108744013 A CN 108744013A CN 201810321841 A CN201810321841 A CN 201810321841A CN 108744013 A CN108744013 A CN 108744013A
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pueraria lobata
solution
silver
preparation
nano silver
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CN108744013B (en
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宋娜
陈建荣
丁林贤
杨利宁
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Zhejiang Normal University CJNU
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Zhejiang Normal University CJNU
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/40—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing ingredients of undetermined constitution or reaction products thereof, e.g. plant or animal extracts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/18—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing inorganic materials
    • A—HUMAN NECESSITIES
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    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
    • A61L15/24—Macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
    • A61L15/26—Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/42—Use of materials characterised by their function or physical properties
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/42—Use of materials characterised by their function or physical properties
    • A61L15/425—Porous materials, e.g. foams or sponges
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/42—Use of materials characterised by their function or physical properties
    • A61L15/44—Medicaments
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    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/42—Use of materials characterised by their function or physical properties
    • A61L15/46—Deodorants or malodour counteractants, e.g. to inhibit the formation of ammonia or bacteria
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
    • D01D5/00—Formation of filaments, threads, or the like
    • D01D5/0007—Electro-spinning
    • D01D5/0015—Electro-spinning characterised by the initial state of the material
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    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/10—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing inorganic materials
    • A61L2300/102—Metals or metal compounds, e.g. salts such as bicarbonates, carbonates, oxides, zeolites, silicates
    • A61L2300/104—Silver, e.g. silver sulfadiazine
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    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
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    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
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Abstract

The preparation method of nano silver provided by the invention-pueraria lobata fiber dressing, includes the following steps：Utilize Ag2SO4Solution carries out degumming process to pueraria lobata fiber, and generates silver-pueraria lobata fibrillar precipitate；Neutralisation processing is carried out to silver-pueraria lobata fibrillar precipitate surface；With the silver after 75% ethanol solution dissolution process-pueraria lobata fibrillar precipitate, ultrasonic vibration is until solution becomes stable yellow solution, formation pueraria lobata fiber-Nano silver intermixture；Polyvinylidene fluoride, dimethyl sulfoxide, fatty acid polyethylene glycol ester, surfactant are added in pueraria lobata fiber-Nano silver intermixture, electrostatic spinning liquid is made to uniform in ultrasonic vibration；Using electrostatic spinning solution spinning, nano silver-pueraria lobata fiber dressing is made.The preparation method of the present invention, makes the flavonoid substances composition in pueraria lobata be retained in a large proportion in pueraria lobata cellulose, make made of dressing hemostasis is rapid, good permeability, strong antibacterial.

Description

The preparation method and application of nano silver-pueraria lobata fiber dressing
Technical field
The invention belongs to Biotechnology field, more particularly to the preparation method of nano silver-pueraria lobata fiber dressing and answer With.
Background technology
Lose blood be the war wound wounded it is dead it is most important due to one of, multi-functional high-end quick hemostatic dressing is The important tool of first aid is carried out under a situation arises in battlefield and daily various wounds.War wound, which is lost blood, is generally divided into internal bleeding and body External hemorrhage, internal bleeding are mostly due to Viscera rupture, and external bleeding is substantially since arteriovenous is damaged.If can have in time The hemostasis of effect ground stablizes the condition of the injury, creates conditions for successive treatment particularly significant to saving wounded's life.Dressing as hemostatic material, Refer to the covering for covering on wound, having protective effect, control bleeding can be assisted, prevent from infecting and absorbing secretion, stopped blooding Dressing has great significance for stopping blooding in time.
Pueraria lobata is legume pueraria lobata, is a kind of often feeding vegetables of some provinces and regions of southern china, taste is sweet, cool and tasty, often It is used as Baoshang.Modern medicine shows：Contain a large amount of Puerarin in pueraria lobata, coronary blood flow can be increased, reduce blood vessel Resistance, adjusting blood circulation, reduction myocardial oxygen consumption etc., are a kind of relatively conventional myocardial preservation substances, energy relieving spasm and pain, prevention Hypertension, dizziness headache, coronary heart diseases and angina pectoris, nervous headache etc..But in fact, in addition to Puerarin, in pueraria lobata not because of kind With at least also containing 12~18% flavonoid substances, Flavonoid substances have expansion peripheral blood vessel, improve microcirculation, dispel saprophytic Flesh, antipyretic convergent effect are first-class hemostatic materials, along with pueraria lobata fiber it is specially treated after, there is tissue looseness, inhale The characteristics such as attached power is strong, the two combines the possibility having in fact as high-end rapid hemostatic material, but the country to rarely having in this respect Research.
Invention content
The purpose of the present invention is in order to solve the above problem, the present invention provides the preparation method of nano silver-pueraria lobata fiber dressing And application.The technical scheme is that be achieved by the following way.
The preparation method for providing nano silver-pueraria lobata fiber dressing according to an aspect of the present invention, includes the following steps：
(1) Ag is utilized2SO4Solution carries out degumming process to pueraria lobata fiber, and generates silver-pueraria lobata fibrillar precipitate；
(2) neutralisation processing is carried out to silver-pueraria lobata fibrillar precipitate surface；
(3) with the silver after 75% ethanol solution dissolution process-pueraria lobata fibrillar precipitate, ultrasonic vibration is until solution becomes Stable yellow solution forms pueraria lobata fiber-Nano silver intermixture；
(4) polyvinylidene fluoride, dimethyl sulfoxide, fatty acid polyglycol ethylene glycol are added in pueraria lobata fiber-Nano silver intermixture Electrostatic spinning liquid is made to uniform in ester, surfactant, ultrasonic vibration；
(5) electrostatic spinning solution spinning is used, nano silver-pueraria lobata fiber dressing is generated.
Wherein, step (1) includes：
Use the H of a concentration of 1~1.5mL/L2SO4Pueraria lobata powder is dissolved, 30~60min in 40 DEG C of water-bath is placed in, is used Tap water is rinsed to neutrality；It adds by the Na2SiO3 solution of a concentration of 30mL/L, the MgSO that mass concentration is 12.75g/L4 Solution, the NaOH solution and H that mass concentration is 40g/mL2O2The mixed liquor being mixed to form, be placed in 60 in 85 DEG C of water-bath~ 80min, stirring；Then the Ag of a concentration of 0.8~1.6g/L is added2SO4Solution is adjusted to neutrality by solution；Yin-Pueraria lobota is obtained by filtration Root fibrillar precipitate.
Wherein, step (1) includes：H2SO4Bath raio with pueraria lobata powder is 5:1, the bath raio of mixed liquor and pueraria lobata powder is 4: 1。
Wherein, step (1) includes：Pueraria lobata is 250g, H2SO4For 1L, Na2SiO3Solution is 30mL, MgSO4Solution is 100mL, NaOH 210mL, H2O2For 660mL, Ag2SO4Solution is 400mL.
Wherein, step (4) includes：Pueraria lobata fiber-Nano silver intermixture：Polyvinylidene fluoride：Dimethyl sulfoxide：Fatty acid polyglycol Glycol ester：Surfactant=270~290：35~45：12~15：1~1.5：1~1.5, the ultrasonic vibration time be 30~ 50min。
Wherein, step (5) includes：Electrostatic spinning voltage is 6~8kV, the distance between syringe needle and receiver board for 10~ 12cm, it is 0.30~0.40mm/h to promote the fltting speed of pump, and spinning environment temperature is 30~35 DEG C.
Wherein, step (2) includes：With warm water washing silver-pueraria lobata fibrillar precipitate, make the table of silver-pueraria lobata fibrillar precipitate Face is neutrality.
Wherein, step (3) includes：60~90min of ultrasonic vibration.
Nano silver-pueraria lobata fiber dressing of preparation method preparation is provided according to another aspect of the present invention in terms of hemostasis Application.
The invention has the advantages that：
1. the preparation method of the present invention, makes flavonoid substances in pueraria lobata be retained in a large proportion in pueraria lobata cellulose, therefore Dressing made of making has good hemostatic function.
2. dressing main ingredient prepared by this method is natural pueraria lobata fiber, the absorbability and material itself to blood are saturating Gas is superior to common hemostatic function material.
3. the present invention combines pueraria lobata fiber, nano silver and high molecular polymer by electrostatic spinning technique, make to be formed Dressing has larger specific surface area and microcellular structure, on the one hand can guide fiber rapidly deeply to wound depths, repair by On the other hand injured tissue makes material have good antibacterial ability, can avoid superinfection.
Specific implementation mode
The illustrative embodiments of the disclosure are described in more detail below.Although showing the disclosure in this specification Illustrative embodiments, it being understood, however, that may be realized in various forms the disclosure without the embodiment party that should be illustrated here Formula is limited.It is to be able to be best understood from the disclosure on the contrary, providing these embodiments, and can be by the disclosure Range is completely communicated to those skilled in the art.
The preparation method and application of 1 nano silver of embodiment-pueraria lobata fiber dressing X1
Include the following steps：
Use the H of a concentration of 1mL/L of 1L2SO4250g pueraria lobata powder is dissolved, 45min in 40 DEG C of water-bath is placed in, with certainly Water is rinsed to the PH most 7 of solution；It adds by the Na of a concentration of 30mL/L of 30mL2SiO3Solution, 100mL mass concentrations are 12.75g/L MgSO4The H of solution, the NaOH solution and 660mL that 210mL mass concentrations are 40g/mL2O2The mixing being mixed to form Liquid is placed in 80min in 85 DEG C of water-bath, stirs 10min；Then the Ag of a concentration of 1.5g/L of 400mL is added2SO4Solution makes Obtain the PH most 7 of solution；.Silver-pueraria lobata fibrillar precipitate is obtained with filtered on buchner funnel；Use 30 DEG C of warm water washing Yin-Pueraria lobota Root fibrillar precipitate, until the pH value on the surface of silver-pueraria lobata fibrillar precipitate is 7.2；After 75% ethanol solution dissolution process Silver-pueraria lobata fibrillar precipitate, ultrasonic vibration 90min to solution becomes stable yellow solution, forms pueraria lobata fiber-Nano silver Intermixture；Polyvinylidene fluoride, dimethyl sulfoxide, fatty acid polyethylene glycol ester, table are added in pueraria lobata fiber-Nano silver intermixture Electrostatic spinning liquid is made, wherein pueraria lobata fiber-Nano silver intermixture to uniform in face activating agent, ultrasonic vibration 30min：Poly- inclined two Vinyl fluoride：Dimethyl sulfoxide：Fatty acid polyethylene glycol ester：Surfactant=270：40：12：1.5：1.5；It is molten using electrostatic spinning Nano silver-pueraria lobata fiber dressing X1 is made in liquid spinning, and wherein electrostatic spinning voltage is 6kV, the distance between syringe needle and receiver board For 10cm, it is 0.40mm/h to promote the fltting speed of pump, and spinning environment temperature is 30 DEG C.
The flavonoid substances content of nano silver-pueraria lobata fiber dressing X1 is surveyed using high performance liquid chromatography (HPLC) Fixed, flavonoid substances content is 15g in X1 after testing, therefore the recovery rate of the preparation method flavones of embodiment 1 is 6%.
The preparation method and application of 2 nano silvers of embodiment-pueraria lobata fiber dressing X2
Include the following steps：
Use the H of a concentration of 1mL/L of 0.4L2SO4100g pueraria lobata powder is dissolved, 45min in 40 DEG C of water-bath is placed in, is used Tap water is rinsed to the PH most 7.3 of solution；It adds by the Na of a concentration of 30mL/L of 12mL2SiO3Solution, 40mL mass concentrations For the MgSO of 12.75g/L4The H of solution, the NaOH solution and 264mL that 84mL mass concentrations are 40g/mL2O2What is be mixed to form is mixed Liquid is closed, 80min in 85 DEG C of water-bath is placed in, stirs 5min；Then the Ag of a concentration of 1.5g/L of 160mL is added2SO4Solution So that the PH most 7.4 of solution；.Silver-pueraria lobata fibrillar precipitate is obtained with filtered on buchner funnel；Use 50 DEG C of warm water washing Silver-pueraria lobata fibrillar precipitate, until the pH value on the surface of silver-pueraria lobata fibrillar precipitate is 7.5；At 75% ethanol solution dissolving Silver-pueraria lobata fibrillar precipitate after reason, ultrasonic vibration 60min to solution become stable yellow solution, form pueraria lobata fiber-and receive The silver-colored intermixture of rice；Polyvinylidene fluoride, dimethyl sulfoxide, fatty acid polyglycol ethylene glycol are added in pueraria lobata fiber-Nano silver intermixture Electrostatic spinning liquid is made, wherein pueraria lobata fiber-Nano silver intermixture to uniform in ester, surfactant, ultrasonic vibration 35min： Polyvinylidene fluoride：Dimethyl sulfoxide：Fatty acid polyethylene glycol ester：Surfactant=290：45：15：1.2：1.5；Use electrostatic Nano silver-pueraria lobata fiber dressing X3 is made in spinning solution spinning, and wherein electrostatic spinning voltage is 8kV, between syringe needle and receiver board Distance be 10cm, promote pump fltting speed be 0.30mm/h, spinning environment temperature be 35 DEG C.
The flavonoid substances content of nano silver-pueraria lobata fiber dressing X2 is surveyed using high performance liquid chromatography (HPLC) Fixed, flavonoid substances content is 6.2g in X2 after testing, therefore the recovery rate of the preparation method flavones of embodiment 2 is 6.2%.
The preparation method and application of 3 nano silvers of embodiment-pueraria lobata fiber dressing X3
Use the H of a concentration of 1mL/L of 0.1L2SO425g pueraria lobata powder is dissolved, 45min in 40 DEG C of water-bath is placed in, with certainly Water is rinsed to the PH most 6.8 of solution；It adds by the Na of a concentration of 30mL/L of 3mL2SiO3Solution, 10mL mass concentrations are 12.75g/L MgSO4The H of solution, the NaOH solution and 66mL that 21mL mass concentrations are 40g/mL2O2The mixing being mixed to form Liquid is placed in 80min in 85 DEG C of water-bath, stirs 7min；Then the Ag of a concentration of 1.5g/L of 40mL is added2SO4Solution makes The PH of solution most 7.1；.Silver-pueraria lobata fibrillar precipitate is obtained with filtered on buchner funnel；Use 50 DEG C of warm water washing Yin-Pueraria lobota Root fibrillar precipitate, until the pH value on the surface of silver-pueraria lobata fibrillar precipitate is 7.1；After 75% ethanol solution dissolution process Silver-pueraria lobata fibrillar precipitate, ultrasonic vibration 80min to solution becomes stable yellow solution, forms pueraria lobata fiber-Nano silver Intermixture；Polyvinylidene fluoride, dimethyl sulfoxide, fatty acid polyethylene glycol ester, table are added in pueraria lobata fiber-Nano silver intermixture Electrostatic spinning liquid is made, wherein pueraria lobata fiber-Nano silver intermixture to uniform in face activating agent, ultrasonic vibration 45min：Poly- inclined two Vinyl fluoride：Dimethyl sulfoxide：Fatty acid polyethylene glycol ester：Surfactant=270：35：14：1：1；It is spun using electrostatic spinning solution Nano silver-pueraria lobata fiber dressing X2 is made in silk, and wherein electrostatic spinning voltage is 8kV, and the distance between syringe needle and receiver board are 12cm, it is 0.40mm/h to promote the fltting speed of pump, and spinning environment temperature is 32 DEG C.
The flavonoid substances content of nano silver-pueraria lobata fiber dressing X3 is surveyed using high performance liquid chromatography (HPLC) Fixed, flavonoid substances content is 1.38g in X2 after testing, therefore the recovery rate of the preparation method flavones of embodiment 2 is 5.52%.
1 blood absorption of experimental example is tested
1. experimental procedures：It weighs 20ml blood to be placed in beaker, cuts out nano silver-pueraria lobata of 1cm*1cm sizes respectively In fiber dressing X1~X3 input beakers, the thickness of wherein nano silver-pueraria lobata fiber dressing X1~X3 is 0.3 millimeter, record It the time required to adsorbing whole blood, cannot such as complete to adsorb, record the maximum adsorption time.
2. experimental results：
Nano silver-pueraria lobata fiber dressing X1~X3 smoothly completes absorption, needed for wherein nano silver-pueraria lobata fiber dressing X1 Time is 8s, is 10s the time required to nano silver-pueraria lobata fiber dressing X2, is 10s the time required to propolis-Nanometer silver dressing X3.
The hemostasis experiment of experimental example 2
1. experimental procedures：The Belgian screech owl rabbit that 30 weight are 6.0~6.3kg is chosen, is randomly divided into 3 groups, every group 10 Only, hemostasis test experiments are carried out.After slowly injecting Nembutal sodium solution anesthesia by 40mg/kg dosage vein, by its central ear Arteriosomes preserved skin, disinfection cut skin along arteria auricularis direction, and blunt separation goes out arteria auricularis, vein and nerve, then uses scalpel The material to be tested and use pull and push dynamometer that transversely cutting artery is immediately 1*1cm with 1 layer of size after blood is gushed out apply 3N Pressure, observe hemostasis every 10s, until record bleeding stopping period after final hemostasis completely, calculate every group used in mean time Between.A kind of wherein every group nano silver-pueraria lobata fiber dressing used in Examples 1 to 3 is as material to be tested.
2. experimental results：
The average bleeding stopping period of nano silver-pueraria lobata fiber X1~X3 is respectively 100s, 105s and 111s.
Experimental example 3
Experiment material：Thickness is nano silver-pueraria lobata fiber dressing X1~X3 and pure cotton fabric Y of 0.620mm, wherein pure The specification of cotton fabric Y is shown in Table 1.
Experimental procedure：It is breathed freely successively to dressing X1~X3 and pure cotton fabric Y using GELLOWEN air permeability testers Degree test, each testing of materials 10 times, it is the air permeability of this kind of material to take mean value.
Experimental result：After testing the average air permeability degree of nano silver-pueraria lobata fiber dressing X1~X3 be respectively 38.7L/min, 32.9L/min and 35.7L/min, and the average air permeability degree of pure cotton fabric Y is 15.1L/min.
In conclusion nano silver-pueraria lobata fiber dressing prepared by the present invention is in addition to excellent absorption and hemostatic capability, Its gas permeability is more better than the pure cotton fabric of same thickness.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto, Any one skilled in the art in the technical scope disclosed by the present invention, the change or replacement that can be readily occurred in, It should be covered by the protection scope of the present invention.Therefore, protection scope of the present invention should be with the protection model of the claim Subject to enclosing.

Claims (9)

1. the preparation method of nano silver-pueraria lobata fiber dressing, which is characterized in that include the following steps：
(1) Ag is utilized2SO4Solution carries out degumming process to pueraria lobata fiber, and generates silver-pueraria lobata fibrillar precipitate；
(2) neutralisation processing is carried out to silver-pueraria lobata fibrillar precipitate surface；
(3) with the silver after 75% ethanol solution dissolution process-pueraria lobata fibrillar precipitate, ultrasonic vibration is until solution becomes stable Yellow solution, formed pueraria lobata fiber-Nano silver intermixture；
(4) polyvinylidene fluoride, dimethyl sulfoxide, fatty acid polyethylene glycol ester, table are added in pueraria lobata fiber-Nano silver intermixture Electrostatic spinning liquid is made to uniform in face activating agent, ultrasonic vibration；
(5) electrostatic spinning solution spinning is used, nano silver-pueraria lobata fiber dressing is made.
2. preparation method as described in claim 1, which is characterized in that the step (1) includes：
Use the H of a concentration of 1~1.5mL/L2SO4Pueraria lobata powder is dissolved, 30~60min in 40 DEG C of water-bath is placed in, with originally Water is rinsed to neutrality；It adds by the Na of a concentration of 30mL/L2SiO3Solution, the MgSO that mass concentration is 12.75g/L4Solution, Mass concentration is the NaOH solution and H of 40g/mL2O2The mixed liquor being mixed to form, be placed in 60 in 85 DEG C of water-bath~ 80min, stirring；Then the Ag of a concentration of 0.8~1.6g/L is added2SO4Solution is adjusted to neutrality by solution；Yin-Pueraria lobota is obtained by filtration Root fibrillar precipitate.
3. preparation method as claimed in claim 2, which is characterized in that the step (1) includes：
Wherein, H2SO4Bath raio with pueraria lobata powder is 5:1, the bath raio of mixed liquor and pueraria lobata powder is 4:1.
4. preparation method as claimed in claim 2, which is characterized in that the step (1) includes：
Wherein, pueraria lobata 250g, H2SO4For 1L, Na2SiO3Solution is 30mL, MgSO4Solution is 100mL, NaOH 210mL, H2O2For 660mL, Ag2SO4Solution is 400mL.
5. preparation method as described in claim 1, which is characterized in that the step (4) includes：
Pueraria lobata fiber-Nano silver intermixture：Polyvinylidene fluoride：Dimethyl sulfoxide：Fatty acid polyethylene glycol ester：Surfactant= (270~290)：(35~45)：(12~15)：(1~1.5)：(1~1.5), ultrasonic vibration time are 30~50min.
6. preparation method as described in claim 1, which is characterized in that the step (5) includes：
Electrostatic spinning voltage is 6~8kV, and the distance between syringe needle and receiver board are 10~12cm, promote the fltting speed of pump to be 0.30~0.40mm/h, spinning environment temperature are 30~35 DEG C.
7. preparation method as described in claim 1, which is characterized in that the step (2) includes：
With warm water washing silver-pueraria lobata fibrillar precipitate, it is neutrality to make the surface of silver-pueraria lobata fibrillar precipitate.
8. preparation method as described in claim 1, which is characterized in that the step (3) includes：
60~90min of ultrasonic vibration.
9. nano silver-pueraria lobata fiber dressing prepared by a kind of preparation method as described in claim 1~8 is any is in terms of hemostasis Application.
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