CN108739865A - A kind of insecticidal suspending agent and preparation method thereof of spring acidalia carticcaria nuclear polyhedrosis virus and muscardine - Google Patents
A kind of insecticidal suspending agent and preparation method thereof of spring acidalia carticcaria nuclear polyhedrosis virus and muscardine Download PDFInfo
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- CN108739865A CN108739865A CN201810764143.8A CN201810764143A CN108739865A CN 108739865 A CN108739865 A CN 108739865A CN 201810764143 A CN201810764143 A CN 201810764143A CN 108739865 A CN108739865 A CN 108739865A
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- spring
- carticcaria
- acidalia
- polyhedrosis virus
- nuclear polyhedrosis
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Environmental Sciences (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Toxicology (AREA)
- Mycology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention provides the insecticidal suspending agent and preparation method thereof of a kind of spring acidalia carticcaria nuclear polyhedrosis virus and muscardine, the suspending agent includes the spring acidalia carticcaria nuclear polyhedrosis virus suspension and beauveria bassiana powder of effective active composition.The insecticidal suspending agent of the spring acidalia carticcaria nuclear polyhedrosis virus and muscardine of the present invention, it is a kind of novel biological pesticide, it is compounded, is overcome merely with Virus latency length and disadvantage individually short with beauveria bassiana preventive effect phase by the virus of specificity, the muscardine of broad spectrum activity and chemical assistant screening study.Viral persistence extended durations of action, control effect are improved, are had no adverse effects to environment, are a kind of safe and non-toxic, high-performance bio pesticides for not making pest develop immunity to drugs.
Description
Technical field
The invention belongs to pesticide fields, and in particular to a kind of desinsection suspension of spring acidalia carticcaria nuclear polyhedrosis virus and muscardine
Agent and preparation method thereof.
Background technology
Spring looper (ApocheimacinerariusErschoff) also known as Mulberry Looper, apocheima cinerarius, main harm poplar, willow,
Elm, Chinese scholartree, mulberry, apple, pears etc..The country is distributed mainly on Xinjiang, Qinghai, Gansu, Shaanxi, Ningxia, the Inner Mongol, Hebei, Tianjin, river
The areas such as south, Shandong, foreign countries are distributed mainly on the thin subregion of the former Soviet Union and the Central Asia.In recent years, single due to the variation of global climate
Tree Species of Plantation large area is planted, along with exchange trade is increasingly frequent between different regions, spring looper in succession Xinjiang, Qinghai,
The area such as the Inner Mongol and Tibet large area occurs, and seriously threatens local natural landscape and ecological environment.
FOREST PROTECT INST CHINA FORE finds a kind of new cause of disease in Hebei Bashang Region, through interior point
From and identification, be confirmed as a kind of spring acidalia carticcaria nuclear polyhedrosis virus (AciNPV), which has not been reported, be state
Inside find for the first time.Two attachmentes of FAO (Food and Agriculture Organization of the United Nation) working paper in agriculture No91 (1973) are pressed by Tianjin health and epidemic prevention station,
With reference to China Ministry of Agriculture, Animal Husbandry and Fisheries file (82) agriculture word the 72nd " about provisions for pesticide registration and food security poison mechanics evaluation journey
Sequence " tests safety, it was demonstrated that the virus is a kind of safe biological insecticides.AciNPV is to parasitize woodland spring ruler
Each instar larvae of earwig and a kind of single-minded insect baculovirus of host, it is by infecting host tissue and in the cell of these tissues
Proliferation, makes to be increased rupture full of polyhedral body in infected tissue nucleus in core, causes host dead, is a kind of important nothing
Nuisance micro-organisms pesticide.AciNPV is persistently effectively to control pest with its safety and environmental protection, long-acting advantage controllable, dosage is few
Preferred biological agent.
Beauveria bassiana (Beaueriabassaria (Balsamo) Vuillemin) is a kind of insect pathogenic fungus, as
Living body biological pesticide, the mass propagation in host insect body make body fluid function change, and metabolic disturbance is so that death.By
It is nontoxic in muscardine, tasteless, pollution-free, it is widely used in agriculture and forestry injurious insect prevention.Muscardine can be used for preventing pine moth, Qi
Scarabaeiform, housefly, tea lesser leafhopper, scale insect, trialeurodes vaporariorum, aphid, thrips, colorado potato beetles, corn borer, longicorn, chafer etc. 40
A variety of agriculture and forestry injurious insects.But there are larger differences for the virulence of different geographic populations or the beauveria bassiana of different strains to pest
It is different, it is differed toxicity of the beauveria bassiana of same strain to variety classes pest, therefore in the biological control of fungi
In often to screen supper toxic strain for different types of pest；In addition, muscardine control effect by ambient humidity influenced compared with
Greatly, when relative humidity is relatively low, control effect is unsatisfactory, and desinsection speed is relatively slow, and it is anti-in agriculture and forestry injurious insect to affect it
The enthusiasm controlled.
Insect viruses are a kind of good biological insecticides, are one of the important means of biological controls.Insect virus insecticide
Agent has high specificity, pathogenicity height, stability good, safe and free from environmental pollution, and the viral disease of pest population can be caused after
It is sick popular to propagate, can natural contral pest growth and decline, and lead to epiphytotics death from generation to generation in succession.But insect virus pesticide is still
There are problems, solves virus multiplication technology and reduce one of the critical issue that production cost is viral merchandized handling.So
And the Biocidal suspending agent of there is not been reported spring acidalia carticcaria nuclear polyhedrosis virus and beauveria bassiana powder compounding.
Invention content
In view of this, the purpose of the present invention is to provide a kind of spring acidalia carticcaria nuclear polyhedrosis virus and the desinsection of muscardine are outstanding
Floating agent, includes the spring acidalia carticcaria nuclear polyhedrosis virus suspension and beauveria bassiana powder of effective active composition, the spring looper core
The weight ratio of type polyhedrosis virus suspension and beauveria bassiana powder is 1:12~2:1；It is highly preferred that the spring looper caryogram is more
The weight ratio of angle precursor virus suspension and beauveria bassiana powder is 1：6~1：2；Most preferably, the spring looper polyhedrosis
The weight ratio of viral suspension and beauveria bassiana powder is 1：3.
Preferably, in spring acidalia carticcaria nuclear polyhedrosis virus of the present invention and the insecticidal suspending agent of muscardine, the spring
A concentration of 20,000,000,000 PIB/mL of acidalia carticcaria nuclear polyhedrosis virus suspension.
Preferably, in spring acidalia carticcaria nuclear polyhedrosis virus of the present invention and the insecticidal suspending agent of muscardine, the ball
A concentration of the 40000000000 of beauveria bassiana powder/gram.
Preferably, described to kill in spring acidalia carticcaria nuclear polyhedrosis virus of the present invention and the insecticidal suspending agent of muscardine
Worm suspending agent further includes silica, phenylalanine, fluorescent whitening agent KSN, sodium lignin sulfonate, chlorfluazuron, glycerine and sterile
Water.
Preferably, described to kill in spring acidalia carticcaria nuclear polyhedrosis virus of the present invention and the insecticidal suspending agent of muscardine
Worm suspending agent includes spring acidalia carticcaria nuclear polyhedrosis virus suspension 5~20% by weight；Beauveria bassiana powder 10~60%；It receives
Meter level silica 0.1~0.5%；Phenylalanine 0.1~0.15%；Fluorescent whitening agent KSN0.5~1%；Sodium lignin sulfonate
0.1~1%；Chlorfluazuron 0.01~0.1%；Glycerine 1.0~5.0%；Surplus is sterile water.
Another aspect of the invention is the preparations for the insecticidal suspending agent for providing spring acidalia carticcaria nuclear polyhedrosis virus and muscardine
Method includes the following steps：
1) spring acidalia carticcaria nuclear polyhedrosis virus suspension is prepared；
2) beauveria bassiana powder is prepared；
3) it weighs：Following components is weighed according to mass percent：Spring acidalia carticcaria nuclear polyhedrosis virus suspension 5~20%；Ball
Beauveria bassiana powder 10~60%；Nanometer grade silica 0.1~0.5%；Phenylalanine 0.1~0.15%；Fluorescent whitening agent
KSN0.5~1%；Sodium lignin sulfonate 0.1~1%；Chlorfluazuron 0.01~0.1%；Glycerine 1.0~5.0%；Surplus is sterile
Water；
4) spring acidalia carticcaria nuclear polyhedrosis virus suspension, beauveria bassiana powder, silica, phenylalanine, glimmering is sequentially added
Optical brightener KSN, sodium lignin sulfonate, chlorfluazuron, glycerine and sterile water are stirred for uniformly dispensing, and spring looper core is prepared
The insecticidal suspending agent of type polyhedrosis virus and muscardine.
Preferably, the preparation method of spring acidalia carticcaria nuclear polyhedrosis virus of the present invention and the insecticidal suspending agent of muscardine
In, the weight ratio of the spring acidalia carticcaria nuclear polyhedrosis virus suspension and beauveria bassiana powder is 1:12~2:1；It is highly preferred that
The weight ratio of the spring acidalia carticcaria nuclear polyhedrosis virus suspension and beauveria bassiana powder is 1：3~2：1；Most preferably, described
Spring acidalia carticcaria nuclear polyhedrosis virus suspension and beauveria bassiana powder weight ratio be 1：1.
Preferably, the preparation method of spring acidalia carticcaria nuclear polyhedrosis virus of the present invention and the insecticidal suspending agent of muscardine
In, a concentration of 20,000,000,000 PIB/mL of the spring acidalia carticcaria nuclear polyhedrosis virus suspension.
Preferably, the preparation method of spring acidalia carticcaria nuclear polyhedrosis virus of the present invention and the insecticidal suspending agent of muscardine
In, the beauveria bassiana powder a concentration of 40,000,000,000/gram.
Compared with prior art, the present invention the present invention has the following advantages：
1) spring acidalia carticcaria nuclear polyhedrosis virus and beauveria bassiana insecticidal suspending agent are a kind of novel biological pesticides, by special
The virus of one property, the muscardine of broad spectrum activity and chemical assistant screening study compound, unexpectedly, such as subsequent embodiment of the present invention
Shown, which shows the synergistic effect prevention spring looper of spring acidalia carticcaria nuclear polyhedrosis virus and muscardine
Advantage, overcome merely with Virus latency length and individually with beauveria bassiana preventive effect phase it is short the shortcomings that.
2) increase the bright protective agent in dosage form, keep dosage form components more perfect.Spring looper polyhedrosis provided by the invention
Virus and beauveria bassiana insecticidal suspending agent, while adding small-scale inorganic nano material, aromatic amino acid and refining on a small quantity glimmering
Optical brightener keeps the component of insect virus pesticide more perfect, and suspension is more preferable, and shelf life is longer, to purple when Field information
Outer light has preferable protective action, viral persistence extended durations of action, control effect to improve.
3) nuclear polyhedrosis virus and beauveria bassiana insecticidal suspending agent, it is nontoxic to people and animals, poultry, fish, plant,
Natural enemy is not injured, is conducive to the ecological balance for safeguarding forest, has no adverse effects to environment, is a kind of safe and non-toxic, does not make pest
The high-performance bio pesticide to develop immunity to drugs.
Specific implementation mode
In one embodiment of the invention, the desinsection for providing a kind of spring acidalia carticcaria nuclear polyhedrosis virus and muscardine is outstanding
Floating agent, includes the spring acidalia carticcaria nuclear polyhedrosis virus suspension and beauveria bassiana powder of effective active composition, the spring looper core
The weight ratio of type polyhedrosis virus suspension and beauveria bassiana powder is 1:12~2:1.
It is highly preferred that in another embodiment of the invention, the spring acidalia carticcaria nuclear polyhedrosis virus suspension and ball spore
The weight ratio of Beauveria Bassiana Powder is 1：3~2：1；Most preferably in another embodiment of the invention, the spring looper caryogram is more
The weight ratio of angle precursor virus suspension and beauveria bassiana powder is 1：1.
It is highly preferred that in another embodiment of the invention, the spring acidalia carticcaria nuclear polyhedrosis virus suspension it is a concentration of
20000000000 PIB/mL.
It is highly preferred that in another embodiment of the invention, the beauveria bassiana powder a concentration of 40,000,000,000/gram.
It is highly preferred that in another embodiment of the invention, the insecticidal suspending agent further includes silica, phenylpropyl alcohol ammonia
Acid, fluorescent whitening agent KSN, sodium lignin sulfonate, chlorfluazuron, glycerine and sterile water.
It is highly preferred that in another embodiment of the invention, the insecticidal suspending agent includes spring looper core by weight
Type polyhedrosis virus suspension 5~20%；Beauveria bassiana powder 10~60%；Nanometer grade silica 0.1~0.5%；Phenylpropyl alcohol ammonia
Acid 0.1~0.15%；Fluorescent whitening agent KSN0.5~1%；Sodium lignin sulfonate 0.1~1%；Chlorfluazuron 0.01~0.1%；It is sweet
Oil 1.0~5.0%；Surplus is sterile water.
Spring acidalia carticcaria nuclear polyhedrosis virus and the insecticidal suspending agent of muscardine are also provided in one more embodiment of the present invention
Preparation method, include the following steps：
1) spring acidalia carticcaria nuclear polyhedrosis virus suspension is prepared；
2) beauveria bassiana powder is prepared；
3) it weighs：Following components is weighed according to mass percent：Spring acidalia carticcaria nuclear polyhedrosis virus suspension 5~20%；Ball
Beauveria bassiana powder 10~60%；Nanometer grade silica 0.1~0.5%；Phenylalanine 0.1~0.15%；Fluorescent whitening agent
KSN0.5~1%；Sodium lignin sulfonate 0.1~1%；Chlorfluazuron 0.01~0.1%；Glycerine 1.0~5.0%；Surplus is sterile
Water；
4) spring acidalia carticcaria nuclear polyhedrosis virus suspension, beauveria bassiana powder, silica, phenylalanine, glimmering is sequentially added
Optical brightener KSN, sodium lignin sulfonate, chlorfluazuron, glycerine and sterile water are stirred for uniformly dispensing, and spring looper core is prepared
The insecticidal suspending agent of type polyhedrosis virus and muscardine.
It is highly preferred that in another embodiment of the invention, the spring acidalia carticcaria nuclear polyhedrosis virus suspension and ball spore
The weight ratio of Beauveria Bassiana Powder is 1:12~2:1；It is highly preferred that the spring acidalia carticcaria nuclear polyhedrosis virus suspension and ball spore are stiff in vain
The weight ratio of bacterium powder is 1：3~2：1；Most preferably, the spring acidalia carticcaria nuclear polyhedrosis virus suspension and beauveria bassiana powder
Weight ratio be 1：1.
It is highly preferred that in another embodiment of the invention, the spring acidalia carticcaria nuclear polyhedrosis virus suspension it is a concentration of
20000000000 PIB/mL.
It is highly preferred that in another embodiment of the invention, the beauveria bassiana powder a concentration of 40,000,000,000/gram.
Below in conjunction with the embodiment in the present invention, the technical solution in the present invention is clearly and completely described.It is aobvious
So, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based on the reality in the present invention
Example is applied, every other embodiment obtained by those of ordinary skill in the art without making creative efforts all belongs to
In the scope of protection of the invention.
The preparation of 1 spring of embodiment acidalia carticcaria nuclear polyhedrosis virus suspension
Spring acidalia carticcaria nuclear polyhedrosis virus, substituted host are spring cankerworm, infect people with 1,000,000 PIB/mL viral suspensions
4 Ling Chun cankerworms of work feed raising, produce spring acidalia carticcaria nuclear polyhedrosis virus；By weight, the people of the spring cankerworm
Work feed formula is：11.2~13.4% wheat germs, 2~5% casein, 0.5~1.8% Webster salt, 0.2~0.43% sorb
Acid, 0.1~0.35% nipalgin, 0.4~0.77% vitamin C, 0.35~0.7% vitamin B, 1.2~1.8% agar,
0.025~0.075% inositol, surplus are distilled water, and the above mass percentage total amount is 100%；Virus infection substituted host
The lethal worm corpse processing of virus is collected within 4 to 10 days afterwards, is processed into the spring acidalia carticcaria nuclear polyhedrosis virus suspension of 20,000,000,000 PIB/mL.
The preparation of 2 beauveria bassiana powder of embodiment
It takes beauveria bassiana strain aseptically to access the common product spore culture medium solid plate of sterilizing to be activated
Processing；It scrapes activated strain and suspension is made, liquid product spore culture medium is added and is enlarged Spawn incubation；It, will after 48 hours
It is connected in the fluid nutrient medium in fermentation tank after sterilizing and carries out liquid fermentation；Culture for 24 hours after, be connected to after material sterilize after solid
In compost, after mixing, solid fermentation is carried out, the good laggard drying shed of solid culture produce spore carries out drying and processing, waits for solid
Body culture thoroughly after drying, carries out the extraction of ball cryptogam on closed extracting machine, and final processing obtains 40,000,000,000/gram white deadlock
Bacterium ball cryptogam.
The preparation of 3 spring of embodiment acidalia carticcaria nuclear polyhedrosis virus and the insecticidal suspending agent of muscardine
It weighs：Following components is weighed according to mass percent：Spring acidalia carticcaria nuclear polyhedrosis virus suspension prepared by embodiment 1
5~20%；Beauveria bassiana powder 10~60% prepared by embodiment 2；Nanometer grade silica 0.1~0.5%；Phenylalanine
0.1~0.15%；Fluorescent whitening agent KSN0.5~1%；Sodium lignin sulfonate 0.1~1%；Chlorfluazuron 0.01~0.1%；Glycerine
1.0~5.0%；Surplus is sterile water, and the above mass percent total amount is 100%；
Sequentially add spring acidalia carticcaria nuclear polyhedrosis virus suspension, beauveria bassiana powder, silica, phenylalanine, fluorescence
Brightening agent KSN, sodium lignin sulfonate, chlorfluazuron, glycerine and sterile water are stirred for uniformly dispensing, and spring looper caryogram is prepared
The insecticidal suspending agent of polyhedrosis virus and muscardine.
Preparation example 1
According to 3 the method for embodiment, the system of spring acidalia carticcaria nuclear polyhedrosis virus and the insecticidal suspending agent of muscardine is carried out
Standby, the content of wherein each component is as follows
Spring acidalia carticcaria nuclear polyhedrosis virus suspension 5%；Beauveria bassiana powder 60% prepared by embodiment 2；Nanoscale dioxy
SiClx 0.1%；Phenylalanine 0.1%；Fluorescent whitening agent KSN1%；Sodium lignin sulfonate 0.5%；Chlorfluazuron 0.1%；Glycerine
5.0%；Surplus is sterile water, and the above mass percent total amount is 100%；
Preparation example 2
With preparation example 1 desinsection of spring acidalia carticcaria nuclear polyhedrosis virus and muscardine is carried out according to 3 the method for embodiment
The preparation of suspending agent differs only in spring acidalia carticcaria nuclear polyhedrosis virus suspension 5%；Beauveria bassiana powder 40%；Nanoscale two
Silica 0.1%；Phenylalanine 0.15%；Fluorescent whitening agent KSN1%；Sodium lignin sulfonate 1.0%；Chlorfluazuron 0.1%；It is sweet
Oil 4.0%；Surplus is sterile water, and the above mass percent total amount is 100%；
Preparation example 3
With preparation example 1 desinsection of spring acidalia carticcaria nuclear polyhedrosis virus and muscardine is carried out according to 3 the method for embodiment
The preparation of suspending agent differs only in spring acidalia carticcaria nuclear polyhedrosis virus suspension 5%；Beauveria bassiana powder 25%；Nanoscale two
Silica 0.5%；Phenylalanine 0.1%；Fluorescent whitening agent KSN0.5%；Sodium lignin sulfonate 0.5%；Chlorfluazuron 0.1%；It is sweet
Oil 3.0%；Surplus is sterile water, and the above mass percent total amount is 100%；
Preparation example 4
With preparation example 1 desinsection of spring acidalia carticcaria nuclear polyhedrosis virus and muscardine is carried out according to 3 the method for embodiment
The preparation of suspending agent differs only in spring acidalia carticcaria nuclear polyhedrosis virus suspension 10%；Beauveria bassiana powder 30%；Nanoscale two
Silica 0.1%；Phenylalanine 0.1%；Fluorescent whitening agent KSN1%；Sodium lignin sulfonate 0.1%；Chlorfluazuron 0.1%；Glycerine
3.0%；Surplus is sterile water, and the above mass percent total amount is 100%；
Preparation example 5
With preparation example 1 desinsection of spring acidalia carticcaria nuclear polyhedrosis virus and muscardine is carried out according to 3 the method for embodiment
The preparation of suspending agent differs only in spring acidalia carticcaria nuclear polyhedrosis virus suspension 15%；Beauveria bassiana powder 15%；Nanoscale two
Silica 0.1%；Phenylalanine 0.15%；Fluorescent whitening agent KSN1%；Sodium lignin sulfonate 0.5%；Chlorfluazuron 0.05%；It is sweet
Oil 2.0%；Surplus is sterile water, and the above mass percent total amount is 100%；
Preparation example 6
With preparation example 1 desinsection of spring acidalia carticcaria nuclear polyhedrosis virus and muscardine is carried out according to 3 the method for embodiment
The preparation of suspending agent differs only in spring acidalia carticcaria nuclear polyhedrosis virus suspension 20%；Beauveria bassiana powder 10%；Nanoscale two
Silica 0.1%；Phenylalanine 0.1%；Fluorescent whitening agent KSN1%；Sodium lignin sulfonate 1.0%；Chlorfluazuron 0.1%；Glycerine
1.0%；Surplus is sterile water, and the above mass percent total amount is 100%；
Preparation example 7
With preparation example 1 desinsection of spring acidalia carticcaria nuclear polyhedrosis virus and muscardine is carried out according to 3 the method for embodiment
The preparation of suspending agent differs only in spring acidalia carticcaria nuclear polyhedrosis virus suspension 5%；Beauveria bassiana powder 5%；Nanoscale dioxy
SiClx 0.1%；Phenylalanine 0.1%；Fluorescent whitening agent KSN0.5%；Sodium lignin sulfonate 0.1%；Chlorfluazuron 0.01%；It is sweet
Oil 1.0%；Surplus is sterile water, and the above mass percent total amount is 100%；
Preparation example 8
With preparation example 1 desinsection of spring acidalia carticcaria nuclear polyhedrosis virus and muscardine is carried out according to 3 the method for embodiment
The preparation of suspending agent differs only in spring acidalia carticcaria nuclear polyhedrosis virus suspension 10%；Beauveria bassiana powder 10%；Nanoscale two
Silica 0.1%；Phenylalanine 0.1%；Fluorescent whitening agent KSN1%；Sodium lignin sulfonate 0.1%；Chlorfluazuron 0.1%；Glycerine
1.0%；Surplus is sterile water, and the above mass percent total amount is 100%；
Preparation example 9
With preparation example 1 desinsection of spring acidalia carticcaria nuclear polyhedrosis virus and muscardine is carried out according to 3 the method for embodiment
The preparation of suspending agent differs only in spring acidalia carticcaria nuclear polyhedrosis virus suspension 20%；Beauveria bassiana powder 20%；Nanoscale two
Silica 0.1%；Phenylalanine 0.15%；Fluorescent whitening agent KSN1%；Sodium lignin sulfonate 0.5%；Chlorfluazuron 0.1%；It is sweet
Oil 2.0%；Surplus is sterile water, and the above mass percent total amount is 100%；
4 Toxicity Determination of embodiment
This test objective is to measure the co-toxicity coefficient of compounding agent (CTC), and CTC < 80 are antagonism, 80≤CTC≤
120 be summation action, and CTC > 120 are synergistic effect.It is dense that the mother liquor of each mixed agent is diluted to five series respectively when experiment
Degree, is respectively placed in spare in beaker.It, will be not in contact with the blade of the same size of any medicament using the method that first leaching leaf is followed by worm
It takes out, naturally dry, is put into insect box after immersion 5s in the liquid configured, then connect for trying larva, in 25 DEG C of items
It is raised under part, often handles 3 repetitions, it is 50 often to repeat test worm number used, while setting blank control, and dead borer population is checked in 72h,
The death rate and corrected mortality are calculated, virulence regression equation is acquired and calculates LC50Value.If compareing the death rate is more than 10%, depending on
To test in vain.Calculation formula is as follows：
Pest corrected mortality is converted into probit value (y), concentration for the treatment of (μ g/ml) is converted into logarithm (x), with minimum
Square law obtains virulence regression equation, and thus calculates the half lethal concentration value of each medicament.According to the abundant equation meters of Sun Yun
Calculate co-toxicity coefficient CTC.Calculation formula is following (using AciNPV as standard agent, toxicity index 100)：
TI × P of theoretical toxicity index (TTI)=AciNPV of MAciNPV+ effectively Beauveria Bassiana Powder the effective muscardines of TI × P
Powder
In formula：M is the mixture of different ratio；
PEffective Beauveria Bassiana PowderFor effective Beauveria Bassiana Powder shared ratio in the composition；
PAciNPVFor AciNPV shared ratios in the composition；
B is Beauveria Bassiana Powder.
Experimental design：The effective lethal dense of AciNPV and muscardine and the two different ratio mixture is determined by pilot study
Spend range.
Toxicity test the results are shown in Table 1：
1 AciNPV of table compounds the toxicity test analysis of results table to spring cankerworm with Beauveria Bassiana Powder
As shown in Table 1, AciNPV prevents the proportioning of spring cankerworm 1 with muscardine compounding：12~2：When 1, poison is altogether
Number is all higher than 180, illustrates the two 1：12~2：Mixture shows synergistic effect in 1 range, when AciNPV and muscardine
Proportioning is 1：3~2：When 1, synergistic effect is more prominent, and co-toxicity coefficient is all higher than 200, wherein as AciNPV and muscardine weight
Than being 1：Co-toxicity coefficient is maximum when 1, and synergistic effect is the most apparent.
5 field trial of embodiment
Before this investigational agent investigate spring cankerworm's insect pest index, in insect pest early period of origination dispenser, 3 days after dispenser, 7 days, 15
It, investigation in 30 days insect pest indexes and calculate preventive effect.Test result is as follows.
Table 2AciNPV and muscardine and its compounding prevention spring cankerworm's test of pesticide effectiveness
As shown in Table 3, to 3 days after dispenser, 7 days, 15 days, 30 days preventive effects, use method of analysis of variance with SPSS softwares
Carry out significance test of difference.
Evaluating drug：
(1) spring acidalia carticcaria nuclear polyhedrosis virus and beauveria bassiana processing all have apparent control effect compared with CK；
(2) spring acidalia carticcaria nuclear polyhedrosis virus and beauveria bassiana compounding agent, than nuclear polyhedrosis virus or ball spore
Effect, which is used alone, in muscardine to get well；
(3) have on lethality between spring acidalia carticcaria nuclear polyhedrosis virus and the various dose of beauveria bassiana compounding certain
Difference, after dispenser 30 days, the preventive effect difference of preparation example 5 and preparation example 9 is not notable, up to 98% or more, from desinsection speed and
Cost-effective comprehensive consideration, it is believed that select preparation example 5 as desinsection optimal dose.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (9)
1. a kind of insecticidal suspending agent of spring acidalia carticcaria nuclear polyhedrosis virus and muscardine includes the spring looper core of effective active composition
Type polyhedrosis virus suspension and beauveria bassiana powder, the spring acidalia carticcaria nuclear polyhedrosis virus suspension and beauveria bassiana powder
Weight ratio is 1:12~2:1.
2. the insecticidal suspending agent of spring acidalia carticcaria nuclear polyhedrosis virus and muscardine according to claim 1, which is characterized in that
A concentration of 20,000,000,000 PIB/mL of the spring acidalia carticcaria nuclear polyhedrosis virus suspension.
3. the insecticidal suspending agent of spring acidalia carticcaria nuclear polyhedrosis virus and muscardine according to claim 1, which is characterized in that
A concentration of the 40000000000 of the beauveria bassiana powder/gram.
4. the insecticidal suspending agent of spring acidalia carticcaria nuclear polyhedrosis virus and muscardine according to claim 1, which is characterized in that
The insecticidal suspending agent further includes silica, phenylalanine, fluorescent whitening agent KSN, sodium lignin sulfonate, chlorfluazuron, glycerine
And sterile water.
5. the insecticidal suspending agent of the spring acidalia carticcaria nuclear polyhedrosis virus and muscardine according to Claims 1 to 4 any one,
It is characterized in that, the insecticidal suspending agent includes spring acidalia carticcaria nuclear polyhedrosis virus suspension 5~20% by weight；Ball spore is white
Stiff bacterium powder 10~60%；Nanometer grade silica 0.1~0.5%；Phenylalanine 0.1~0.15%；Fluorescent whitening agent KSN0.5
~1%；Sodium lignin sulfonate 0.1~1%；Chlorfluazuron 0.01~0.1%；Glycerine 1.0~5.0%；Surplus is sterile water.
6. the preparation method of a kind of spring acidalia carticcaria nuclear polyhedrosis virus and the insecticidal suspending agent of muscardine, includes the following steps：
1) spring acidalia carticcaria nuclear polyhedrosis virus suspension is prepared；
2) beauveria bassiana powder is prepared；
3) it weighs：Following components is weighed according to mass percent：Spring acidalia carticcaria nuclear polyhedrosis virus suspension 5~20%；Ball spore is white
Stiff bacterium powder 10~60%；Nanometer grade silica 0.1~0.5%；Phenylalanine 0.1~0.15%；Fluorescent whitening agent KSN0.5
~1%；Sodium lignin sulfonate 0.1~1%；Chlorfluazuron 0.01~0.1%；Glycerine 1.0~5.0%；Surplus is sterile water；
4) spring acidalia carticcaria nuclear polyhedrosis virus suspension, beauveria bassiana powder, silica, phenylalanine, fluorescence is sequentially added to increase
White agent KSN, sodium lignin sulfonate, chlorfluazuron, glycerine and sterile water, are stirred for uniformly dispensing, it is more that spring looper caryogram are prepared
The insecticidal suspending agent of angle precursor virus and muscardine.
7. the preparation method of spring acidalia carticcaria nuclear polyhedrosis virus according to claim 6 and the insecticidal suspending agent of muscardine,
It is characterized in that, the weight ratio of the spring acidalia carticcaria nuclear polyhedrosis virus suspension and beauveria bassiana powder is 1:12~2:1.
8. the preparation method of spring acidalia carticcaria nuclear polyhedrosis virus according to claim 6 and the insecticidal suspending agent of muscardine,
It is characterized in that, a concentration of 20,000,000,000 PIB/mL of the spring acidalia carticcaria nuclear polyhedrosis virus suspension.
9. the preparation method of spring acidalia carticcaria nuclear polyhedrosis virus according to claim 6 and the insecticidal suspending agent of muscardine,
It is characterized in that, a concentration of the 40000000000 of the beauveria bassiana powder/gram.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1471826A (en) * | 2003-07-08 | 2004-02-04 | 中国林业科学研究院森林保护研究所 | Mass production of virus insecticides |
| CN107197861A (en) * | 2016-08-31 | 2017-09-26 | 江西省科学院微生物研究所 | A kind of CPV and the insecticidal suspending agent of muscardine and preparation method thereof |
| CN107821440A (en) * | 2017-10-20 | 2018-03-23 | 江西省科学院微生物研究所 | Insecticidal suspending agent that a kind of tea-geometrid-type polyhedrosis viruses compound with beauveria bassiana powder and its preparation method and application |
| CN107926986A (en) * | 2017-11-30 | 2018-04-20 | 江西省科学院微生物研究所 | A kind of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus and muscardine insecticidal suspending agent and its preparation method and application |
-
2018
- 2018-07-12 CN CN201810764143.8A patent/CN108739865A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1471826A (en) * | 2003-07-08 | 2004-02-04 | 中国林业科学研究院森林保护研究所 | Mass production of virus insecticides |
| CN107197861A (en) * | 2016-08-31 | 2017-09-26 | 江西省科学院微生物研究所 | A kind of CPV and the insecticidal suspending agent of muscardine and preparation method thereof |
| CN107821440A (en) * | 2017-10-20 | 2018-03-23 | 江西省科学院微生物研究所 | Insecticidal suspending agent that a kind of tea-geometrid-type polyhedrosis viruses compound with beauveria bassiana powder and its preparation method and application |
| CN107926986A (en) * | 2017-11-30 | 2018-04-20 | 江西省科学院微生物研究所 | A kind of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus and muscardine insecticidal suspending agent and its preparation method and application |
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Application publication date: 20181106 |