CN108732081A - A kind of immunocyte instrument fluid system - Google Patents
A kind of immunocyte instrument fluid system Download PDFInfo
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- CN108732081A CN108732081A CN201710248878.0A CN201710248878A CN108732081A CN 108732081 A CN108732081 A CN 108732081A CN 201710248878 A CN201710248878 A CN 201710248878A CN 108732081 A CN108732081 A CN 108732081A
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- 239000012530 fluid Substances 0.000 title claims abstract description 31
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 89
- 210000004369 blood Anatomy 0.000 claims abstract description 86
- 239000008280 blood Substances 0.000 claims abstract description 86
- 238000006243 chemical reaction Methods 0.000 claims abstract description 48
- 238000005070 sampling Methods 0.000 claims abstract description 29
- 239000007788 liquid Substances 0.000 claims abstract description 22
- 238000004140 cleaning Methods 0.000 claims abstract description 15
- 239000003219 hemolytic agent Substances 0.000 claims abstract description 15
- 239000012895 dilution Substances 0.000 claims abstract description 13
- 238000010790 dilution Methods 0.000 claims abstract description 13
- 238000001514 detection method Methods 0.000 claims abstract description 12
- 239000002699 waste material Substances 0.000 claims abstract description 12
- 210000004027 cell Anatomy 0.000 claims description 14
- 238000010438 heat treatment Methods 0.000 claims description 8
- 238000004458 analytical method Methods 0.000 claims description 7
- 238000010241 blood sampling Methods 0.000 claims description 6
- 239000007850 fluorescent dye Substances 0.000 claims description 6
- 238000000691 measurement method Methods 0.000 claims description 6
- 239000003795 chemical substances by application Substances 0.000 claims description 5
- 230000009466 transformation Effects 0.000 claims description 5
- 238000001917 fluorescence detection Methods 0.000 claims description 4
- 238000011534 incubation Methods 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 230000003287 optical effect Effects 0.000 claims description 4
- GNFTZDOKVXKIBK-UHFFFAOYSA-N 3-(2-methoxyethoxy)benzohydrazide Chemical compound COCCOC1=CC=CC(C(=O)NN)=C1 GNFTZDOKVXKIBK-UHFFFAOYSA-N 0.000 claims description 3
- FGUUSXIOTUKUDN-IBGZPJMESA-N C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 Chemical compound C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 FGUUSXIOTUKUDN-IBGZPJMESA-N 0.000 claims description 3
- 210000000601 blood cell Anatomy 0.000 claims description 3
- 239000000243 solution Substances 0.000 claims description 2
- 235000013399 edible fruits Nutrition 0.000 claims 1
- 150000003384 small molecules Chemical class 0.000 abstract description 3
- 241000894006 Bacteria Species 0.000 abstract description 2
- 230000035945 sensitivity Effects 0.000 abstract description 2
- 238000005516 engineering process Methods 0.000 description 4
- 230000005284 excitation Effects 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000002203 pretreatment Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 230000005611 electricity Effects 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 206010018910 Haemolysis Diseases 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000004159 blood analysis Methods 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 230000005283 ground state Effects 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000011527 multiparameter analysis Methods 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
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- 239000000376 reactant Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1434—Optical arrangements
- G01N15/1436—Optical arrangements the optical arrangement forming an integrated apparatus with the sample container, e.g. a flow cell
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1404—Handling flow, e.g. hydrodynamic focusing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
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- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention discloses a kind of immunocyte instrument fluid systems, it is characterized in that, it including sample flow and injector for collecting blood, hemolytic agent syringe, sampling needle cleaning syringe, sheath stream syringe, sampling needle service sink syringe, blood taking needle, adopts reagent needle, negative pressure chamber, blood taking needle service sink, adopt reagent needle service sink, reaction tank, reagent storage silo, flow chamber, filter, B/P EGR Back Pressure Transducer EGR, waste liquid pool and dilution bucket, each device is connected to form complete cell instrument fluid system by pump, valve, connector and pipeline.Small molecule project can detect using the present invention and expand to the detection of the sundry items such as bacterium, high sensitivity, extension is good, easy to operate, efficient.
Description
Technical field
The present invention relates to a kind of flow cytometers, and in particular to a kind of immunocyte instrument fluid system.
Background technology
Flow cytometer is to carry out quick, multi parameter analysis INVENTIONModern cell analytical technology to suspension cell or particle, is led to
Overflow-type cell art control cell one by one by the blood analysis instrument of detection zone, flow cytometry typically refer to cell stream (
Referred to as sample flow) under the package of sheath fluid, the cell fluid control technology in good order by flow chamber detection zone, which can
The problem of causing to detect misalignment by detection zone by irregular route to avoid cell, while cell overlap can be greatly reduced
The probability of missing inspection is generated by detection zone.The quasi-instrument is currently used primarily in cell sorting and quantitative use.Streaming is thin at present
The sample flow of born of the same parents' instrument generally uses syringe to provide, and sheath flowing power source is constant pressure air source.Constant pressure air source advantage be can accomplish and
When respond, provide stable sheath stream in the short time, but main disadvantage is not easy to safeguard, is with high costs, thus be unfavorable for big model
Popularization is enclosed, causes its application space limited.In addition the pre-treatment of current flow cytometer showed instrument is required to hand adaptation other instruments
It completes, an instrument cannot be integrated in and completed, cumbersome, speed is slow.
Existing immunity analysis instrument includes mainly two parts, i.e. immune response system and chemiluminescence analysis system.It is immune
Reaction system is that being marked directly on antigen, (chemiluminescence is exempted from by luminescent substance (the generation excitation state intermediate under reactant excitation)
Epidemic disease is analyzed) or antibody (immunochemiluminometry) on or enzyme effect in luminous substrate, the formation of the intermediate needs specific
Temperature and corresponding time, it is therefore desirable to provide.Chemiluminescence analysis system is to utilize catalysis of the chemiluminescent substance through catalyst
With the oxidation of oxidant, the intermediate of an excitation state is formed, when this excitation state intermediate returns to stable ground state, simultaneously
Launch photon, measures the quantity of light quantum using luminous signal measuring instrument to judge result.Immunoassay at present
Instrument sensitivity is not enough to carry out the detection of small molecule, and test speed is not high.
Therefore, the existing technology needs to be improved and developed.
Invention content
The purpose of the present invention is to provide a kind of immunocyte instrument fluid systems, it is intended to solve existing flow cytometer behaviour
Make the problem that complicated, efficiency is low, stability is poor and application range is limited.
Technical scheme is as follows：
A kind of immunocyte instrument fluid system comprising sample flow and injector for collecting blood, hemolytic agent syringe, sampling needle are clear
Syringe is washed, sheath stream syringe, sampling needle service sink syringe, blood taking needle, reagent needle is adopted, negative pressure chamber, blood taking needle service sink, adopts
Reagent needle service sink, reaction tank, reagent storage silo, flow chamber, filter, B/P EGR Back Pressure Transducer EGR, waste liquid pool and dilution bucket, it is described
Each device is connected to form complete cell instrument fluid system by pump, valve, connector and pipeline.
The immunocyte instrument fluid system, wherein the sheath stream syringe is separately connected by third triple valve
Two three-way connections and the 7th three-way connection；Second three-way connection is respectively communicated with the 6th two-way valve and the first three-way connection again,
6th two-way valve connects the sample flow and injector for collecting blood, described other two interface of first three-way connection pass through the 4th
Two-way valve and the 5th two-way valve are connected to the inlet of flow chamber and blood taking needle respectively；7th three-way connection connects dilution
Entrance；The hemolytic agent syringe is respectively communicated with the thief hatch and third three-way connection for adopting reagent needle, institute by the first triple valve
It states third three-way connection and is separately connected the second triple valve and the 4th three-way connection, the 4th three-way connection is separately connected the four or three
Port valve and the 7th three-way connection, the 7th three-way connection connect dilution entrance；Sampling needle cleaning syringe passes through the
Two triple valves are respectively communicated with third three-way connection and adopt the inlet of reagent needle；The sampling needle service sink syringe passes through the 4th
Triple valve is respectively communicated with the 4th three-way connection and the 5th triple valve, and the 5th triple valve connects blood taking needle service sink and adopts reagent
Needle service sink；The blood taking needle service sink was connected to by the first two-way valve and the second two-way valve respectively with reagent needle service sink is adopted
5th three-way connection and the 8th three-way connection；8th three-way connection connects the first breeches joint by the first pump and is connected to waste liquid
Mouthful；5th three-way connection connects the 6th three-way connection, first pump of the 6th three-way connection connection and the 11st two-way valve, institute
It states the second pump and connects the first breeches joint connection waste liquid port；The sample flow is connected to stream with injector for collecting blood by the 7th two-way valve
The sample inlet of dynamic room；The thief hatch of the blood taking needle is connected to the sample inlet of flow chamber by the 8th two-way valve；It is described
The waste liquid outlet of flow chamber connects the inlet of negative pressure chamber by third two-way valve, and negative pressure sensing is provided in the negative pressure chamber
Device, the negative pressure chamber also connect the second breeches joint by the 12nd two-way valve, and second breeches joint connects filter；It is described
The liquid outlet of negative pressure chamber connects the 6th three-way connection by the 11st two-way valve；The rinse liquid outlet of the blood taking needle passes through the tenth
Two-way valve is connected to the 8th three-way connection；The rinse liquid outlet for adopting reagent needle is connected by the 9th two-way valve connection the five or three
Head.
The immunocyte instrument fluid system, wherein measurement method is：
Step A：The reagent and fluorescent dye of needs are taken by adopting reagent needle from reagent storage silo；
Step B：After having adopted reagent, adopts reagent needle and enter in reaction tank, separate corresponding positions in specified reagent to reaction tank
There are several disposal reagent kits in the kit set, in reaction tank, is carried out down after the good reagent of candidate agent box point and fluorescent dye
One step divides blood；
Step C：By sample flow and injector for collecting blood and blood taking needle, blood was collected；It reagent needle will be adopted will be put into simultaneously and adopt reagent
Needle service sink is cleaned；
Step D：After the completion of blood sampling, blood taking needle enters in reaction tank, separates the examination in specified blood sample to reaction tank successively
In agent box；
Step E：Reaction tank lid is covered, there is heating plate heating in reaction tank, sample is incubated under temperature constant state；Together
When by blood taking needle be put into blood sampling service sink clean；
Step F：In equal reaction tanks sample blending incubation for a period of time after, pass through sample flow and injector for collecting blood and the 8th liang
Reaction tank is sucked out in the sample being incubated by port valve；
Step G：Open third triple valve, the 4th two-way valve, the 6th two-way valve and the 7th two-way valve；Close the 6th two-way valve
With the 8th two-way valve；Push the sample flow to form sheath stream package into becoming a mandarin by sample flow and injector for collecting blood and sheath stream syringe
Dynamic room exports final after flowing indoor fluorescence detection device to optical signal collection processing by computer photoelectric signal transformation
As a result.
The immunocyte instrument fluid system, wherein measurement method is：
Step a：The reagent of needs is taken by adopting reagent needle from reagent storage silo；
Step b：After having adopted reagent, adopts reagent needle and enter in reaction tank, then separate in specified reagent to reaction tank, instead
Ying Chizhong has each and every one several disposal reagent kits, carries out dividing blood in next step after waiting for the good reagent of all kits point；
Step c：By sample flow and injector for collecting blood and blood taking needle, blood was collected；The entrance of reagent needle will be adopted simultaneously adopts reagent
Needle service sink is cleaned；
Step d：After having taken a blood sample, blood taking needle enters in reaction tank, then separates successively in specified blood sample to reaction tank
In kit；
Step e：Reaction tank lid is covered, there is heating plate heating in reaction tank, sample is incubated under temperature constant state；It adopts
Blood needle enters blood sampling service sink and is cleaned；
Step f：Sample blending incubation after a certain period of time, passes through sample flow and injector for collecting blood and the 8th liang in equal reaction tanks
Reaction tank is sucked out in the sample being incubated by port valve；
Step g：Open third triple valve, the 4th two-way valve and the 7th two-way valve；Close the 5th two-way valve, the 6th two-way valve
With the 8th two-way valve；Streamer emitter pushes dilution to enter flow chamber, and sample flow and injector for collecting blood push sample, be formed simultaneously
Sheath stream is wrapped up blood sample and is lined up through flow chamber progress blood cell analysis detection, after fluorescence detection device handles optical signal collection,
Final result is exported by computer photoelectric signal transformation.
The immunocyte instrument fluid system, wherein the hemolytic agent syringe, sampling needle cleaning syringe, sheath stream
The driving of syringe and use needle service sink syringe shares first motor, and the sample flow and injector for collecting blood pass through the second electricity
Machine drives.
The immunocyte instrument fluid system, wherein the sheath stream syringe, hemolytic agent syringe, sampling needle cleaning
The arrangement of syringe and sampling needle service sink syringe meets equalising torque requirement, and the first torque and the second torque are equal.
Beneficial effects of the present invention：The present invention by being integrated with reaction tank on immunocyte instrument fluid system, reagent is deposited
Warehouse so that the pre-treatment of sample can be completed in an instrument, easy to operate, efficient；By to sheath stream syringe, haemolysis
Agent syringe, sampling needle cleaning syringe, sampling needle service sink syringe carry out equalising torque arrangement, and it is steady to improve driving
Property and motor service life, relative to air source drive, save cost；The sheath stream syringe, hemolytic agent syringe, sampling needle
The shared motor of syringe, sampling needle service sink syringe is cleaned, four schemes are dragged as a result of one, only needs a driving electricity
Machine makes the driving device of motor also accordingly reduce three sets, again reduces cost；Further, since the presence of four syringes,
Integral rigidity and inertia increase, and can buffer motor-driven gap uneven stability, obtain syringe scheme more single than driving more
Stable sample flow improves the accuracy of detection of fluidic cell.Sheath stream and sample flow are all made of syringe promotion, and equipment is made to be more easy to
In operation, flexibility reduces cost while enhancing, is conducive to larger range of popularization.
Description of the drawings
Fig. 1 is the structural schematic diagram of this immunocyte instrument fluid system.
Fig. 2 is a kind of measuring method flow chart of the immunocyte instrument fluid system.
Fig. 3 is another measuring method flow chart of the immunocyte instrument fluid system.
Specific implementation mode
To make the objectives, technical solutions, and advantages of the present invention clearer and more explicit, develop simultaneously embodiment pair referring to the drawings
The present invention is further described.
Refering to fig. 1, immunocyte instrument fluid system of the invention includes mainly：
Sample flow and injector for collecting blood 25, hemolytic agent syringe 26, sampling needle cleaning syringe 27, sheath stream syringe 28,
Sampling needle service sink syringe 29, blood taking needle 30 adopt reagent needle 31, negative pressure chamber 32, blood taking needle service sink 40, adopt the cleaning of reagent needle
Pond 41, reaction tank 47, reagent storage silo 48, flow chamber 49, filter 53, B/P EGR Back Pressure Transducer EGR 54, waste liquid pool 33 and dilution bucket
34.Each device is connected to form complete cell instrument fluid system by pump, valve, connector and pipeline.To reduce cost, institute
State hemolytic agent syringe 26, sampling needle cleaning syringe 27, sheath stream syringe 28 and the driving using needle service sink syringe 29
First motor 45 is shared, the sample flow and injector for collecting blood 25 are driven by the second motor 46.
Wherein valve includes：First two-way valve LV5, the second two-way valve LV6, third two-way valve LV8, the 4th two-way valve LV9,
5th two-way valve LV10, the 6th two-way valve LV11, the 7th two-way valve LV12, the 8th two-way valve LV13, the 9th two-way valve LV14,
Ten two-way valve LV15, the 11st two-way valve LV16 and the 12nd two-way valve LV17；First triple valve LV1, the second triple valve LV2,
Third triple valve LV3, the 4th triple valve LV4 and the 5th triple valve LV7.
Its center tap includes：First three-way connection 18, the second three-way connection 19, third three-way connection the 20, the 4th 3 connect
First 21, the 5th three-way connection 22, the 6th three-way connection 23, the 7th three-way connection 35, the 8th three-way connection 36；First breeches joint
24 and second breeches joint 37.
Wherein pump includes：First pump 42 and the second pump 43.
The sheath stream syringe 28 is separately connected the second three-way connection 19 and the 7th three-way connection by third triple valve LV3
35；Second three-way connection 19 is respectively communicated with the 6th two-way valve LV11 and the first three-way connection 18, the 6th two-way valve again
The LV11 connections sample flow and injector for collecting blood 25, first three-way connection, 18 other two interface pass through the 4th two-way valve
LV9 and the 5th two-way valve LV10 are connected to the inlet of flow chamber 49 and blood taking needle 30 respectively；7th three-way connection 35 connects
Connect dilution entrance 51.
The hemolytic agent syringe 26 is respectively communicated with the thief hatch and the three or three for adopting reagent needle 31 by the first triple valve LV1
Pass joint 20, the third three-way connection 20 are separately connected the second triple valve LV2 and the 4th three-way connection 21, the 4th threeway
Connector 21 is separately connected the 4th triple valve LV4 and the 7th three-way connection 35, and the 7th three-way connection 35 connects dilution entrance
51。
The sampling needle cleaning syringe 27 is respectively communicated with third three-way connection 20 by the second triple valve LV2 and adopts reagent
The inlet of needle 31.
The sampling needle service sink syringe 29 is respectively communicated with the 4th three-way connection 21 and the 5th by the 4th triple valve LV4
Triple valve LV7, the 5th triple valve LV7 connection blood taking needles service sink 40 and adopts reagent needle service sink 41.The blood taking needle is clear
Wash pool 40 with adopt reagent needle service sink 41 and be connected to the five or three by the first two-way valve LV5 and the second two-way valve LV6 respectively and connect
First 22 and the 8th three-way connection 36；8th three-way connection 36 is connected to waste liquid by 42 the first breeches joint 24 of connection of the first pump
Mouthful；5th three-way connection 22 connects the 6th three-way connection 23, first pump 43 and the 10th of the 6th three-way connection 23 connection
Port valve LV16, second pump, 43 the first breeches joint 24 of connection are connected to waste liquid port.
The sample flow is connected to the sample inlet of flow chamber 49 with injector for collecting blood 25 by the 7th two-way valve LV12.Institute
The thief hatch for stating blood taking needle 30 is connected to the sample inlet of flow chamber 49 by the 8th two-way valve LV13.The flow chamber 49 gives up
Liquid exports 52 inlets by third two-way valve LV8 connections negative pressure chamber 32, and B/P EGR Back Pressure Transducer EGR is provided in the negative pressure chamber 32
54, also by the 12nd the second breeches joint of two-way valve LV17 connections 37, second breeches joint 37 connected for the negative pressure chamber
Filter 53.The liquid outlet of the negative pressure chamber 32 passes through the 6th three-way connection 23 of the 11st two-way valve LV16 connections.
The rinse liquid outlet of the blood taking needle 30 is connected to the 8th three-way connection 36 by the tenth two-way valve LV15.It is described to adopt examination
The rinse liquid outlet of agent needle 31 is connected to the 5th three-way connection 22 by the 9th two-way valve LV14.
Referring again to Fig. 1, immunocyte instrument fluid system provided by the invention is integrated with reaction tank, reagent storage silo, makes
Obtaining the pre-treatment of sample can complete in an instrument, easy to operate, efficient；The sheath stream syringe, hemolytic agent syringe,
Sampling needle cleans syringe, the arrangement of sampling needle service sink syringe meets equalising torque requirement, the first torque L1 and the second power
Square L2 is equal, for that can improve driving stationarity and motor service life, is driven relative to air source, saves cost；The sheath
Streamer emitter, hemolytic agent syringe, sampling needle cleaning syringe, sampling needle service sink syringe share a motor, due to adopting
Four schemes are dragged with one, a driving motor is only needed, so that the driving device of motor is also accordingly reduced three sets, reduce cost,
Further, since the presence of four syringes, integral rigidity and inertia increase, it is unstable that motor-driven gap can be buffered
Property, syringe scheme more single than driving more stably sample flow is obtained, the accuracy of detection of fluidic cell is improved；Sheath stream and sample flow
It is all made of syringe promotion, equipment is made to be physically easier to perform, flexibility reduces cost while enhancing, is conducive to larger range of
It promotes.
Referring to Fig.2, based on the immunocyte instrument fluid system, the present invention also provides a kind of effective measurement method, tools
Steps are as follows for body：
Step A：The reagent and fluorescent dye of needs are taken by adopting reagent needle 31 from reagent storage silo 48；
Step B：After having adopted reagent, adopts reagent needle 31 and enter in reaction tank 47, separate in specified reagent to reaction tank 47
In the kit of corresponding position, there are several disposal reagent kits, candidate agent box point good reagent and fluorescent dye in reaction tank 47
It carries out dividing blood in next step afterwards；
Step C：By sample flow and injector for collecting blood 25 and blood taking needle 30, blood was collected；Reagent needle 31 will be adopted simultaneously to be put into
Reagent needle service sink 41 is adopted to be cleaned；
Step D：After the completion of blood sampling, blood taking needle 30 enters in reaction tank 47, separates specified blood sample successively to reaction tank 47
In kit in；
Step E：Reaction tank lid is covered, there is heating plate heating in reaction tank 47, sample is incubated under temperature constant state；
Blood taking needle 30 blood sampling service sink 40 is put into simultaneously to clean；
Step F：Sample blending incubation after a certain period of time, passes through sample flow and injector for collecting blood 25 and the in equal reaction tanks 47
Reaction tank is sucked out in the sample being incubated by eight two-way valve LV 13；
Step G：Open third triple valve LV 3, the 4th two-way valve LV 9, the 5th two-way valve LV 10 and the 7th two-way valve LV
12；Close the 6th two-way valve LV 11 and the 8th two-way valve LV 13；Pass through sample flow and injector for collecting blood 25 and sheath stream syringe
28 push formed sheath stream package sample flow enter flow chamber 49, the fluorescence detection device in flow chamber 49 to optical signal collection at
After reason, final result is exported by computer photoelectric signal transformation.
Refering to Fig. 3, it is preferred that based on the immunocyte instrument fluid system, the present invention also provides another preferred
Measurement method is as follows：
Step a：The reagent of needs is taken by adopting reagent needle 31 from reagent storage silo 48；
Step b：After having adopted reagent, adopts reagent needle 31 and enter in reaction tank 47, then separate specified reagent to reaction tank
There are several disposal reagent kits in 47, in reaction tank, carries out dividing blood in next step after waiting for the good reagent of all kits point；
Step c：By sample flow and injector for collecting blood 25 and blood taking needle 30, blood was collected；Reagent needle 31 will be adopted simultaneously to enter
Reagent needle service sink 41 is adopted to be cleaned；
Step d：After having taken a blood sample, blood taking needle 30 enters in reaction tank 47, then separates specified blood sample successively to reaction tank
In kit in 47；
Step e：Reaction tank lid is covered, there is heating plate heating in reaction tank 47, sample is incubated under temperature constant state；
Blood taking needle 30 enters blood sampling service sink 40 and is cleaned；
Step f：Sample blending incubation after a certain period of time, passes through sample flow and injector for collecting blood 25 and the in equal reaction tanks 47
Reaction tank is sucked out in the sample being incubated by eight two-way valve LV13；
Step g：Open third triple valve LV 3, the 4th two-way valve LV 9 and the 7th two-way valve LV 12；The 5th liang is closed to lead to
Valve LV 10, the 6th two-way valve LV 11 and the 8th two-way valve LV 13；Streamer emitter 28 pushes dilution to enter flow chamber 49, sample
This stream and injector for collecting blood 25 push sample, are formed simultaneously sheath stream package blood sample queuing and carry out blood cell analysis by flow chamber 49
Detection.After fluorescence detection device handles optical signal collection, final result is exported by computer photoelectric signal transformation.
For example 1 with example 2 difference lies in the push mode that sample flow enters flow chamber, scheme one is injected using sheath stream
Device auxiliary pushes away sample, so as to quickly form sheath stream.Scheme is second is that sheath stream syringe pushes away sample and sheath stream with quickly by blood simultaneously
Sample is pushed into flow chamber 49.
The immunocyte system for the distribution of commodities provided by the invention and its usability methods have following prominent compared to currently existing scheme
The advantages of：
1) immunity analysis instrument of the invention can detect small molecule project and expand to the detection of the sundry items such as bacterium, sensitive
Degree is high, and extension is good；
2) Sample pretreatment, sample reaction and sample measurement are integrated in an instrument and complete, easy to operate, efficient；
3) detectable substance is marked in then quantitative, fluorescent marker stabilization by fluorescent marker, it can long-term preservation.
It should be understood that the application of the present invention is not limited to the above for those of ordinary skills can
With improvement or transformation based on the above description, all these modifications and variations should all belong to the guarantor of appended claims of the present invention
Protect range.
Claims (6)
1. a kind of immunocyte instrument fluid system, which is characterized in that including sample flow and injector for collecting blood, hemolytic agent syringe,
Sampling needle cleaning syringe, sampling needle service sink syringe, blood taking needle, adopts reagent needle, negative pressure chamber, blood taking needle at sheath stream syringe
Service sink adopts reagent needle service sink, reaction tank, reagent storage silo, flow chamber, filter, B/P EGR Back Pressure Transducer EGR, waste liquid pool and dilution
Liquid bucket, each device are connected to form complete cell instrument fluid system by pump, valve, connector and pipeline.
2. immunocyte instrument fluid system according to claim 1, which is characterized in that the sheath stream syringe passes through third
Triple valve is separately connected the second three-way connection and the 7th three-way connection；Second three-way connection is respectively communicated with the 6th two-way valve again
With the first three-way connection, the 6th two-way valve connects the sample flow and injector for collecting blood, and first three-way connection is in addition
Two interfaces are connected to the inlet of flow chamber and blood taking needle respectively by the 4th two-way valve and the 5th two-way valve；Described 7th 3
Pass joint connects dilution entrance；The hemolytic agent syringe be respectively communicated with by the first triple valve adopt reagent needle thief hatch and
Third three-way connection, the third three-way connection are separately connected the second triple valve and the 4th three-way connection, and the described 4th 3 connects
Head is separately connected the 4th triple valve and the 7th three-way connection, and the 7th three-way connection connects dilution entrance；The sampling needle
Cleaning syringe is respectively communicated with third three-way connection by the second triple valve and adopts the inlet of reagent needle；The sampling needle cleaning
Pond syringe is respectively communicated with the 4th three-way connection and the 5th triple valve, the 5th triple valve connection blood sampling by the 4th triple valve
Needle service sink and adopt reagent needle service sink；The blood taking needle service sink and adopt reagent needle service sink respectively by the first two-way valve and
Second two-way valve was connected to the 5th three-way connection and the 8th three-way connection；8th three-way connection connects first by the first pump
Breeches joint is connected to waste liquid port；5th three-way connection connects the 6th three-way connection, the first pump of the 6th three-way connection connection and the
11 two-way valves, the second pump first breeches joint of connection are connected to waste liquid port；The sample flow and injector for collecting blood pass through the 7th
Two-way valve is connected to the sample inlet of flow chamber；The thief hatch of the blood taking needle is connected to the sample of flow chamber by the 8th two-way valve
Inlet；The waste liquid outlet of the flow chamber connects the inlet of negative pressure chamber by third two-way valve, is arranged in the negative pressure chamber
There are B/P EGR Back Pressure Transducer EGR, the negative pressure chamber also to connect the second breeches joint, the second breeches joint connection by the 12nd two-way valve
Filter；The liquid outlet of the negative pressure chamber connects the 6th three-way connection by the 11st two-way valve；The cleaning solution of the blood taking needle
Outlet is connected to the 8th three-way connection by the tenth two-way valve；The rinse liquid outlet for adopting reagent needle is connected to by the 9th two-way valve
5th three-way connection.
3. immunocyte instrument fluid system according to claim 1, which is characterized in that measurement method is：
Step A：The reagent and fluorescent dye of needs are taken by adopting reagent needle from reagent storage silo；
Step B：It after having adopted reagent, adopts reagent needle and enters in reaction tank, separate corresponding position in specified reagent to reaction tank
There are several disposal reagent kits in kit, in reaction tank, is carried out in next step after the good reagent of candidate agent box point and fluorescent dye
Divide blood；
Step C：By sample flow and injector for collecting blood and blood taking needle, blood was collected；Reagent needle will be adopted simultaneously is put into that adopt reagent needle clear
Wash pool is cleaned；
Step D：After the completion of blood sampling, blood taking needle enters in reaction tank, separates the kit in specified blood sample to reaction tank successively
In；
Step E：Reaction tank lid is covered, there is heating plate heating in reaction tank, sample is incubated under temperature constant state；Simultaneously will
Blood taking needle is put into blood sampling service sink and is cleaned；
Step F：In equal reaction tanks sample blending incubation for a period of time after, pass through sample flow and injector for collecting blood and the 8th two-way valve
Reaction tank is sucked out in the sample being incubated；
Step G：Open third triple valve, the 4th two-way valve, the 6th two-way valve and the 7th two-way valve；Close the 6th two-way valve and
Eight two-way valves；The sample flow to form sheath stream package is pushed to enter flowing by sample flow and injector for collecting blood and sheath stream syringe
Room most terminates after flowing indoor fluorescence detection device to optical signal collection processing by the output of computer photoelectric signal transformation
Fruit.
4. immunocyte instrument fluid system according to claim 1, which is characterized in that measurement method is：
Step a：The reagent of needs is taken by adopting reagent needle from reagent storage silo；
Step b：After having adopted reagent, adopts reagent needle and enter in reaction tank, then separate in specified reagent to reaction tank, reaction tank
In have each and every one several disposal reagent kits, carry out dividing blood in next step after waiting for all kits point good reagent；
Step c：By sample flow and injector for collecting blood and blood taking needle, blood was collected；It is clear into reagent needle is adopted that reagent needle will be adopted simultaneously
Wash pool is cleaned；
Step d：After having taken a blood sample, blood taking needle enters in reaction tank, then separates the reagent in specified blood sample to reaction tank successively
In box；
Step e：Reaction tank lid is covered, there is heating plate heating in reaction tank, sample is incubated under temperature constant state；Blood taking needle
It is cleaned into blood sampling service sink；
Step f：Sample blending incubation after a certain period of time, passes through sample flow and injector for collecting blood and the 8th two-way valve in equal reaction tanks
Reaction tank is sucked out in the sample being incubated；
Step g：Open third triple valve, the 4th two-way valve and the 7th two-way valve；Close the 5th two-way valve, the 6th two-way valve and
Eight two-way valves；Streamer emitter pushes dilution to enter flow chamber, and sample flow and injector for collecting blood push sample, be formed simultaneously sheath stream
It wraps up blood sample to be lined up through flow chamber progress blood cell analysis detection, after fluorescence detection device handles optical signal collection, pass through
Computer photoelectric signal transformation exports final result.
5. immunocyte instrument fluid system according to claim 1, which is characterized in that the hemolytic agent syringe, sampling
Needle cleans syringe, sheath stream syringe and shares first motor using the driving of needle service sink syringe, the sample flow and adopts
Blood syringe is driven by the second motor.
6. immunocyte instrument fluid system according to claim 5, which is characterized in that the sheath stream syringe, hemolytic agent
Syringe, sampling needle cleaning syringe and sampling needle service sink syringe arrangement meet equalising torque requirement, the first torque and
Second torque is equal.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710248878.0A CN108732081A (en) | 2017-04-17 | 2017-04-17 | A kind of immunocyte instrument fluid system |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710248878.0A CN108732081A (en) | 2017-04-17 | 2017-04-17 | A kind of immunocyte instrument fluid system |
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| CN109580321A (en) * | 2018-11-27 | 2019-04-05 | 迪瑞医疗科技股份有限公司 | A kind of fluid path air bubble eliminating device and method |
| WO2020227855A1 (en) * | 2019-05-10 | 2020-11-19 | 深圳迈瑞生物医疗电子股份有限公司 | Sample detection device and operating method for sample detection device |
| CN112881723A (en) * | 2019-11-30 | 2021-06-01 | 深圳市帝迈生物技术有限公司 | Blood detection device and blood detection method |
| CN113759137A (en) * | 2020-06-04 | 2021-12-07 | 深圳市帝迈生物技术有限公司 | Sample detection device and sample detection method |
| WO2022011265A3 (en) * | 2020-07-10 | 2022-02-17 | Idexx Laboratories Inc. | Point-of-care medical diagnostic analyzer and devices, systems, and methods for medical diagnostic analysis of samples |
| CN114113571A (en) * | 2020-08-27 | 2022-03-01 | 深圳市帝迈生物技术有限公司 | Immunoassay analyzer, liquid path system thereof and cleaning method of liquid path system |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109580321A (en) * | 2018-11-27 | 2019-04-05 | 迪瑞医疗科技股份有限公司 | A kind of fluid path air bubble eliminating device and method |
| CN109580321B (en) * | 2018-11-27 | 2021-05-18 | 迪瑞医疗科技股份有限公司 | Liquid path bubble removing device and method |
| WO2020227855A1 (en) * | 2019-05-10 | 2020-11-19 | 深圳迈瑞生物医疗电子股份有限公司 | Sample detection device and operating method for sample detection device |
| CN112881723A (en) * | 2019-11-30 | 2021-06-01 | 深圳市帝迈生物技术有限公司 | Blood detection device and blood detection method |
| CN112881723B (en) * | 2019-11-30 | 2022-12-23 | 深圳市帝迈生物技术有限公司 | Blood detection device and blood detection method |
| CN113759137A (en) * | 2020-06-04 | 2021-12-07 | 深圳市帝迈生物技术有限公司 | Sample detection device and sample detection method |
| WO2022011265A3 (en) * | 2020-07-10 | 2022-02-17 | Idexx Laboratories Inc. | Point-of-care medical diagnostic analyzer and devices, systems, and methods for medical diagnostic analysis of samples |
| US11977091B2 (en) | 2020-07-10 | 2024-05-07 | Idexx Laboratories Inc. | Point-of-care medical diagnostic analyzer and devices, systems, and methods for medical diagnostic analysis of samples |
| CN114113571A (en) * | 2020-08-27 | 2022-03-01 | 深圳市帝迈生物技术有限公司 | Immunoassay analyzer, liquid path system thereof and cleaning method of liquid path system |
| CN114113571B (en) * | 2020-08-27 | 2023-12-15 | 深圳市帝迈生物技术有限公司 | Immunoassay analyzer, liquid path system thereof and cleaning method of liquid path system |
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Application publication date: 20181102 |