CN1087299C - Method for producing active oligomeric xylose - Google Patents
Method for producing active oligomeric xylose Download PDFInfo
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- CN1087299C CN1087299C CN99105722A CN99105722A CN1087299C CN 1087299 C CN1087299 C CN 1087299C CN 99105722 A CN99105722 A CN 99105722A CN 99105722 A CN99105722 A CN 99105722A CN 1087299 C CN1087299 C CN 1087299C
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Abstract
The present invention provides a method for producing active oligomeric xylose. In the method, straws are used as raw materials, and processed through steam explosion treatment, water extraction, purification and concentration to obtain the active oligomeric xylose. The method has the advantages of cheap raw material, easy raw material obtainment, simple production process, no pollution, high product yield, low production cost, etc.
Description
The present invention relates to produce the method for active oligomeric xylose.
In recent years, in global functional foodstuff upsurge, oligose gains great popularity.In the molecule of oligose, by 2--10 monose with glycosidic link be connected [He Zhaofan, Zhang Diqing, protective foods chemistry and detection technique thereof, China Light Industry Press, Beijing, 1998,14--17].Be divided into active oligose and ordinary oligosaccharide two big classes by its purposes, active oligose is meant oligose [Bezkorovainy, A. et al., Int. J. Biochem., 1981, the 13:585-592 that people, animal and plant is had the special physiological function.Spiegel, J. E., Food Technol., 1994, (1): 85--92], its major physiological effect has: (1) is not is not digested and assimilated, and the energy that provides is very low or do not have at all, is difficult for fermenting for spoilage organism; (2) the activation and proliferation factor of bifidus bacillus in the enteron aisle suppresses the growth of harmful intestinal tract spoilage organism; (3) can not cause carious tooth, help oral hygiene; (4) have the physiological function of food fibre, but be easy to eat than food fibre; (5) active oligose of part such as xylo-oligosaccharide, oligomeric galactose aldehydic acid have the effect of plant growth regulation and inducing plant generation antibiotics.And ordinary oligosaccharide such as sucrose, lactose, maltose, trisaccharide maltose and maltotetrose can be digested and assimilated, and are not the activation and proliferation factors of intestinal bifidobacteria.
The special physiological function of active oligose has caused the bifid somatomedin effect of the attention, particularly active oligose of academia and industrial community.The SANZHU KOUFUYE of home sale, ANGLI No.1 etc. are the ecological microbial agents based on bifidus bacillus, and active oligose is compared with ecological microbial agent as the bifid somatomedin, have oxytolerant acidproof, can together take, be difficult for advantage [Sakai such as inactivation, production cost be low with microbiotic, K., et al., Agric. Biol. Chem., 1987,51 (2): 315--322].Be widely used in food-processing, healthcare products and the medicine at the active oligose of Japan, the sales volume of 19 kinds of active oligose reaches 4.7 ten thousand tons, and the output value surpasses 10,000,000,000 yen [in happiness brightness husband, use the saccharic science, 1995,42 (3): 275--283] for a long time.Current China food configuration is reasonable inadequately, and the functional foodstuff DEVELOPMENT PROSPECT is wide.Now domesticly only malto-oligosaccharide, dextrinosan and stachyose have been carried out research and produced [Jin Qirong, foodstuffs industry, 1995, (3): 5--6].The production method of active oligose mainly contains 5 kinds, and [build etc. in horizontal field, make association, 1994,89 (8): 626--683]: (1) natural matter extraction method----is extracted from sugar beet juice as raffinose, soybean oligosaccharide extracts from soybean is clear, because content is low in the natural matter, extraction method is subjected to the restriction of raw material.(2) acid hydrolysis natural polysaccharide method-----owing to acid-hydrolyzed poor selectivity, carbohydrate complexity in hydrolyzed solution is difficult to obtain specific oligose.(3) chemical synthesis----only limits to the functional study of oligose at present owing to the production cost height.(4) enzyme engineering method----is utilized the shift reaction or the lytic enzyme hydrolysis natural polysaccharide of the functional group of transferring enzymes.Because the selectivity of enzyme is strong, easily obtains specific oligose, pollution-free, production cost is low, so become the method for industrial practicality.
Xylo-oligosaccharide is meant the active oligose that is connected and composed with β-1,4 glycosidic link by 2--7 wood sugar.Compare with other active oligose, tool is indigestibility more, stronger bifidus bacillus proliferation activity, only need picked-up 0.7 gram just can reach whole intestines effect (general active oligose consumption is 2--10 gram/people/day) for each person every day, to Heat stability is good, strong (pH2.5) [Chen Ruijuan, the food and fermentation industries of acid resistance, 1993, (2): 82--90].External at present this sugar of production is raw material with corn cob, bagasse, cotton seed hull etc. mainly, at first goes out xylan with the alkali extracting, uses xylanase hydrolysis then, forms [rich field room man, chemical と biology, 1992,30 (3): 170--174] through separation and purification again.This method easily produces environmental pollution, production process complexity, the product yield is low, production cost is high.Xylo-oligosaccharide is by Japanese Suntory Ltd. Development and Production as stated above, in listing in 1989.In molasses product, xylo-oligosaccharide content is more than 70%, 2500 yen/kilogram of prices; Xylo-oligosaccharide content is more than 20% in the pulverulent product, 1900 yen/kilogram of prices.Still do not have the xylo-oligosaccharide product in China and sell, the domestic report [Cai Jing equality, microbiology circular, 1997,24 (2): 91--94] that the chamber of experimentizing research is as stated above only arranged.
The existing quick-fried technology of vapour is mainly used in aspects such as papermaking, feed processing, fiberboard manufacturing.Before the present invention makes, all useless both at home and abroad in the report of producing xylo-oligosaccharide.
The method that the purpose of this invention is to provide a kind of new production xylo-oligosaccharide.Present method has overcome contaminate environment that prior art exists, production process complexity, the product yield is low, production cost is high shortcoming.
The invention provides a kind of method of producing active oligomeric xylose.This method is handled agricultural crop straw based on steam explosion, can make wherein the xylan isolating characteristics of optionally degrading, the novel method of opening up Applied Physics principle system xylo-oligosaccharide.The main component of agricultural crop straw is Mierocrystalline cellulose, hemicellulose and xylogen, and wherein the primary structure framework of hemicellulose is a polyxylose.The quick-fried processing of vapour can make the polyxylose degraded of hemicellulose separate, thereby makes the hemicellulose tool water-soluble, separates through water extracting and insoluble Mierocrystalline cellulose and xylogen with water.This solubility hemicellulose main component is an xylo-oligosaccharide, through the chromatographic separation purifying that it can be decoloured, obtains purified xylo-oligosaccharide after the concentration.Technical process is as follows: stalk → rough lumber (20-30mm is long) → steam explosion → water extracting → chromatographic separation and purification → concentration → 50% active oligomeric xylose ↓ extracting slag (fermentation protein feedstuff)
In the method for the present invention, stalk can be agricultural byproducts such as straw, wheat straw, corn stalk, corn cob, beanstalk, sorghum stalks, bagasse, cotton seed bar.Also comprise simultaneously the plant material that other hemicellulose moiety is a polyxylose.Contained polyxylose component is high more good more.
In the method for the present invention, steam explosion refer to pack in the steam-explosion jar of the certain scale straw of rough lumber (20-30mm is long) is handled 2-6 minute (it is better generally to handle 4 minutes effects in 1.5Mpa) in 1.0-1.8Mpa, and instant decompression discharges and promptly gets the quick-fried straw of vapour.
In the method for the present invention, the water extracting requires certain process conditions: 10-20%, 50-80 ℃ of hot-water soaks of the quick-fried straw concentration of vapour 0-8 hour.According to the raw material difference, optimum condition is different.Preferably adopt multistage extraction technology: repeat formula extracting and continous way extracting for several times.Repeat for several times the formula extracting and be meant extracting gained filtrate is used further to the new steam puffed stalk of extracting, thereby improve extract sugar concentration.The continous way extracting is meant material and the continuous reverse input of water in operating process, can control the residual quantity of solvend in extract concentration and the extracting slag, is easy to automatization.
In the method for the present invention, the chromatography separation and purification comprises gac or the multiple chromatography mode such as macroporous resin or dextrane gel of adopting.According to different material, select to use.All can be undertaken application of sample amount, elution speed, eluent etc. in the chromatography process by condition well known in the prior art.Concentration can adopt nanometer filter or methods such as ultrafiltration, thin film evaporation or reduction vaporization.
At present both at home and abroad prior art exists shortcomings such as contaminate environment, production process complexity, product yield are low, production cost height.The novel process that steam puffed stalk extracts active oligomeric xylose has overcome above-mentioned shortcoming, have production process simple (having simplified alkaline extraction xylan, the production preparation of zytase and the enzymic hydrolysis process of xylan), pollution-free (Gu the shape slag is as fermentation protein feedstuff raw material), product yield height (in the quick-fried process of vapour, xylan is loss not, the xylan rate of recovery 80%), the low advantages such as (the quick-fried process of vapour only consume steam, and its consumption is 0.5 ton of steam/ton wheat stalk) of production cost.
Embodiment: steam exploded wheat straw is produced active oligomeric xylose
1. steam exploded wheat straw
At 1M
3In the steam-explosion jar, the long wheat straw 80Kg of about 25mm that packs into handled 4 minutes in 1.5Mpa, and instant decompression discharges and promptly gets steam exploded wheat straw.
2. the water extracting quick-fried wheat straw of vapour
Carried out suction filtration under the condition in 4 hours in steam exploded wheat straw concentration 15%, 75 ℃ of hot-water soaks.Adopt and repeat the formula extracting four times, also be about to extracting gained filtrate and be used further to the new steam exploded wheat straw of extracting.Can make extract sugar concentration reach 92.6g/l.(if adopt four continous way extractings, that is in operating process material and the continuous reverse input of water, can make extract sugar concentration reach 35.12g/l).
3. the extract purifying is an active oligomeric xylose
This extract heating is concentrated into 1/4th of its volume, getting certain amount is added to and gives on the activated carbon chromatography post that installs earlier (5-10 that general column length is a column diameter doubly is advisable), with 60% alcohol wash-out, the elutriant of collecting is with 3, the calibrating of 5-dinitrosalicylic acid method, can stop to collect to no longer containing reducing sugar, mixing the elutriant contain in the reducing sugar pipe is active oligomeric xylose (this elutriant is analyzed with HPLC, its spectrogram is consistent with 70% xylo-oligosaccharide HPLC spectrogram of enzymolysis process production with Japan, proves that this reducing sugar is xylo-oligosaccharide).
4. the concentration of active oligomeric xylose
Under 40 ℃ of temperature condition, collected active oligomeric xylose is carried out reduction vaporization to remove wherein contained ethanol (recyclable repeated use), promptly obtain containing the molasses product of active oligomeric xylose 50% again through the nanometer filter.The biological activity that this product is water white thickness pulpous state, light sweet taste, do not have other peculiar smell, tool is stronger, with respect to the raw material dry weight, the yield of active oligomeric xylose is 10%-15%.
Claims (4)
1. method of producing active oligomeric xylose, it is characterized in that: this method is to be raw material with the straw-like materials, handle by steam explosion, again through water extracting, purifying, concentrate, finally obtain active oligomeric xylose; Said steam explosion is handled the stalk that is meant the chopping of packing in steam-explosion jar, handles 2-6 minute in 1.0---1.8Mpa, and instant decompression discharges and promptly gets steam puffed stalk; Said water extracting be meant steam puffed stalk concentration 10-20%, 50-80 ℃ hot-water soak 0-8 hour, adopt the mode of multistage extraction to carry out.
2. it is characterized in that in accordance with the method for claim 1: said straw-like materials is straw, wheat straw, cornstalk, corn cob, beanstalk, sorghum straw, bagasse or cotton seed stalk.
3. it is characterized in that in accordance with the method for claim 1: said purifying is meant and adopts gac or macroporous resin or sephadex chromatography mode to carry out.
4. it is characterized in that in accordance with the method for claim 1: said concentrating is meant what employing nanometer filter or ultrafiltration, thin film evaporation or reduction vaporization carried out.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99105722A CN1087299C (en) | 1999-04-12 | 1999-04-12 | Method for producing active oligomeric xylose |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99105722A CN1087299C (en) | 1999-04-12 | 1999-04-12 | Method for producing active oligomeric xylose |
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| CN1233614A CN1233614A (en) | 1999-11-03 |
| CN1087299C true CN1087299C (en) | 2002-07-10 |
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| CN99105722A Expired - Fee Related CN1087299C (en) | 1999-04-12 | 1999-04-12 | Method for producing active oligomeric xylose |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100402658C (en) * | 2005-03-21 | 2008-07-16 | 中国科学院过程工程研究所 | Method and apparatus for preparing reducing sugar by enzymolysis of steam-exploded straw with membrane reactor |
| CN101148630B (en) * | 2006-09-19 | 2010-12-08 | 中国科学院过程工程研究所 | Method for preparing microorganism grease by fermenting steam-exploded straw hemicellulose hydrolysate |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1116419C (en) * | 1999-12-16 | 2003-07-30 | 合肥联合大学 | Enzymolysis process for preparing functional oligoxylose |
| CN1303091C (en) * | 2004-04-05 | 2007-03-07 | 山东龙力生物科技有限公司 | Preparation of oligo-wood sugar |
| CN100422736C (en) * | 2005-12-08 | 2008-10-01 | 上海复星临西药业有限公司 | Method for detecting oligo-xylose in oligo-xylose tablets |
| CN101074247B (en) * | 2007-03-28 | 2010-08-04 | 李来锁 | Selective vegetable glucosidic bond high-energy bursting production method |
| CN101289682B (en) * | 2007-04-20 | 2012-03-21 | 中粮集团有限公司 | Process for preparing monosaccharide by using cellulose-containing raw material |
| CN102071267B (en) * | 2010-12-15 | 2012-09-12 | 广东石油化工学院 | Method for coproducing xylose, white carbon black and active carbon from rice hulls |
| CN107129542A (en) * | 2017-07-10 | 2017-09-05 | 桂林融通科技有限公司 | A kind of method for extracting bagasse polysaccharide |
| CN108354061B (en) * | 2018-02-28 | 2021-07-16 | 苏州昆蓝生物科技有限公司 | Production method of intestinal regulator |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10117800A (en) * | 1996-08-30 | 1998-05-12 | Akita Pref Gov | Production of monosaccharide, oligosaccharide and solubilized polysaccharide |
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1999
- 1999-04-12 CN CN99105722A patent/CN1087299C/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10117800A (en) * | 1996-08-30 | 1998-05-12 | Akita Pref Gov | Production of monosaccharide, oligosaccharide and solubilized polysaccharide |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100402658C (en) * | 2005-03-21 | 2008-07-16 | 中国科学院过程工程研究所 | Method and apparatus for preparing reducing sugar by enzymolysis of steam-exploded straw with membrane reactor |
| CN101148630B (en) * | 2006-09-19 | 2010-12-08 | 中国科学院过程工程研究所 | Method for preparing microorganism grease by fermenting steam-exploded straw hemicellulose hydrolysate |
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| CN1233614A (en) | 1999-11-03 |
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Assignee: Shandong Fengyuan Zhongke Ecology Technology Co., Ltd. Assignor: Institute of Process Engineering, Chinese Academy of Sciences Contract fulfillment period: 2009.9.20 to 2016.1.20 contract change Contract record no.: 2009370000494 Denomination of invention: Method for producing active oligomeric xylose Granted publication date: 20020710 License type: Exclusive license Record date: 2009.11.5 |
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Free format text: EXCLUSIVE LICENSE; TIME LIMIT OF IMPLEMENTING CONTACT: 2009.9.20 TO 2016.1.20; CHANGE OF CONTRACT Name of requester: SHANDONG FENGYUAN ZHONGKE ECOLOGY SCIENCE AND TECH Effective date: 20091105 |
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Granted publication date: 20020710 Termination date: 20130412 |