Puncher for microbial strains
The invention relates to a divisional application of a patent with the application number of 2016108651090 and the application date of 2016, 09 and 25, and named as a puncher for a microbial strain.
Technical Field
The invention relates to the technical field of microbial culture devices, in particular to a microbial strain hole puncher.
Background
In the production and scientific research experiments of microorganisms, strains are inoculated into a plate culture medium, and after the strains grow to a certain area in the culture medium, the plate containing the strains is formed. At this time, a punch is commonly used to take a uniform-sized clump (culture) from a plate containing the bacteria into fresh medium or gel. At present, the puncher that conventionally used exists when removing the easy landing of fungus piece from the puncher, the difficult follow puncher that breaks away from of fungus piece during the inoculation, and operating speed is slow, and work efficiency is low shortcoming, and when punching and inoculation, the culture dish lid is opened too big or time overlength, causes miscellaneous fungus to pollute easily.
Disclosure of Invention
The invention aims to solve the problems in the prior art and provide a puncher for microbial strains, which can conveniently punch and take out a bacterium block under the condition that a culture dish is not easy to pollute sundry bacteria, the bacterium block is not easy to slide off from the puncher during movement, and the bacterium block is easy to separate from the puncher during inoculation.
The invention relates to a puncher for microbial strains, which comprises a conical bucket body, wherein an inclined handheld rod is fixedly connected to the outer side wall of the upper end of the conical bucket body, the bottom of the conical bucket body is hinged to a bottom cover, two sides of one end of the bottom cover are hinged to the side wall of the bottom of the conical bucket body through hinge shafts, the bottom of the other end of the bottom cover is fixed to one end of an arc-shaped lifting rod, a guide ring is formed on the side wall of the bottom of the conical bucket body between the hinge shafts, an arc-shaped insertion groove is formed in the bottom cover, the insertion groove is inserted into the guide ring, an annular reset torsion spring is inserted into the guide ring, one end of the reset torsion spring is fixed to the guide ring, and the other end of the reset torsion spring is fixed to the side wall of the insertion groove;
the inclined handheld rod is provided with an inclined supporting rod which forms an acute angle with the inclined handheld rod, the lower end of the supporting rod is provided with a guide groove, a guide block is inserted in the guide groove, the upper end of the guide block is fixed with a supporting rod, the lower end of the guide block is fixed with a connecting rod, the connecting rod penetrates through an inclined avoiding groove in the inclined handheld rod and is fixedly connected to one end of the lifting rod, the upper end of the supporting rod penetrates through a positioning block, the positioning block is fixed to the upper end of the supporting rod, a spring is inserted and sleeved on the supporting rod, two ends of the spring are pressed on the positioning block and the guide block respectively, a rack portion is formed on the side wall of the supporting rod, one part of the rack portion is inserted into the spring, the other part of the rack portion penetrates through the positioning block and is meshed with an adjusting gear, and the adjusting gear is hinged to the supporting rod.
By the technical scheme, when the puncher is used, an operator holds the inclined hand-held rod and stirs the adjusting gear to enable the adjusting gear to rotate, the adjusting gear drives the rack part meshed with the adjusting gear to move upwards, the rack part drives the supporting rod to move upwards, the supporting rod moves upwards to drive the guide block to move upwards in the guide groove of the supporting rod, the spring is compressed, the supporting rod drives the connecting rod to move upwards, the connecting rod drives the arc-shaped lifting rod hinged to the connecting rod to move upwards, one end of the lifting rod drives the bottom cover at the bottom of the conical bucket body to rotate around the hinged part of the conical bucket body, in the process, the inserting groove on the bottom cover rotates around the guide ring and compresses the reset torsion spring, so that the bottom of the conical bucket body is opened, then the conical bucket body is pressed downwards on a bacterial containing flat plate to punch the bacterial blocks, the conical bucket body is lifted upwards and the adjusting gear is loosened after punching is completed, and under the dual functions of the spring and the reset torsion spring, connecting rod and lifting rod reset, drive the bottom round with the toper bucket body articulated department reset rotatory, the torsional spring that resets makes the inserting groove rotatory on the guide ring, closes the bottom at the toper bucket body until the bottom covers again, so make the fungus piece can not fall out from the toper bucket body, remove the toper bucket body to fresh culture medium or gel top after that, invert the toper bucket body through the oblique handheld pole of reversal to move the fungus piece to in fresh culture medium or the gel.
Through the scheme, the microbial strain puncher disclosed by the invention is not easy to punch and take out a bacterial block under the condition that a culture dish is not polluted by sundry bacteria, the bacterial block is not easy to slide off from the puncher during movement, and the bacterial block is easy to separate from the puncher during inoculation.
Preferably as one of above-mentioned scheme, the upper end shaping of bracing piece has the hinge groove, be fixed with the articulated shaft on the lateral wall around the hinge groove, the middle part plug bush of adjusting gear is in on the articulated shaft, the one end of adjusting gear is worn out the rack portion meshing of hinge groove and branch, and the other end is worn out the hinge groove and is fixed with sectorial protective sheath, the shaping has convex key of stirring on the lateral wall of protective sheath.
Preferably, the guide block is formed with two limiting blocks, and the two limiting blocks respectively abut against two opposite side walls of the support rod.
Preferably, the supporting rod is a round rod, the supporting rod is parallel to the supporting rod, and the central axis of the supporting rod and the central axis of the connecting rod are located on the same straight line.
Preferably, a reinforcing rib is fixedly connected between the support rod and the inclined handheld rod.
Preferably, an elastic buffer layer is formed on the top surface of one end of the bottom cover fixedly connected with the lifting rod.
Preferably, a handle is fixed to a rear end of the diagonal hand-held rod, and a stopper is fixed to the diagonal hand-held rod in front of the handle.
The foregoing description is only an overview of the technical solutions of the present invention, and in order to make the technical solutions of the present invention more clearly understood and to implement them in accordance with the contents of the description, the following detailed description is given with reference to the preferred embodiments of the present invention and the accompanying drawings.
Drawings
The drawings are only for purposes of illustrating and explaining the present invention and are not to be construed as limiting the scope of the present invention. Wherein:
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic partial structure view of the top view of FIG. 1;
FIG. 3 is a partial cross-sectional view of FIG. 1;
FIG. 4 is another partial cross-sectional view of FIG. 1;
fig. 5 is a schematic view of the working state of fig. 4.
Detailed Description
The following detailed description of embodiments of the present invention is provided in connection with the accompanying drawings and examples. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
Referring to fig. 1, 2 and 4, the microbial strain puncher of the invention comprises a conical bucket body 10, wherein an inclined hand-held rod 20 is fixedly connected to the outer side wall of the upper end of the conical bucket body, a handle 21 is fixed to the rear end of the inclined hand-held rod, a stop block 22 is fixed to the inclined hand-held rod 20 at the front side of the handle, a bottom cover 30 is hinged to the bottom of the conical bucket body 10, two sides of one end of the bottom cover are hinged to the side wall of the bottom of the conical bucket body 10 through hinge shafts 11, the bottom of the other end of the bottom cover is fixed to one end of an arc-shaped lifting rod 40, an elastic buffer layer 31 is formed on the top surface of one end of the bottom cover 30 fixedly connected with the lifting rod 40, a guide ring 12 is formed on the side wall of the bottom of the conical bucket body 10 between the hinge shafts 11, an arc-shaped insertion groove 32 is formed on the bottom cover 30, the insertion groove is inserted on the guide ring 12, an annular reset torsion spring 50 is inserted on the guide ring, one end of the reset torsion spring is fixed on the guide ring 12, and the other end is fixed on the side wall of the insertion groove 32.
Referring to fig. 1 and 3, an inclined support rod 60 forming an acute angle with the inclined handheld rod 20 is formed on the inclined handheld rod 20, a reinforcing rib 23 is fixedly connected between the support rod and the inclined handheld rod 20, a guide groove 61 is formed at the lower end of the support rod 60, a guide block 70 is inserted in the guide groove, two limit blocks 71 are formed on the guide block and respectively abut against two opposite side walls of the support rod 60, a support rod 80 is fixed at the upper end of the guide block 70, a connecting rod 90 is fixed at the lower end of the guide block, the connecting rod passes through an inclined avoidance groove 24 formed on the inclined handheld rod 20 and is fixedly connected to one end of the lifting rod 40, the support rod 80 is a round rod, the support rod 80 is parallel to the support rod 60, the central axis of the support rod 80 and the central axis of the connecting rod 90 are positioned on the same straight line, the upper end of the support rod 80 passes through a positioning block 62, the positioning block is fixed at the upper end of the support rod 60, the supporting rod 80 is inserted and sleeved with a spring 100, two ends of the spring are respectively pressed on the positioning block 62 and the guide block 70, a rack part 81 is formed on the side wall of the supporting rod 80, one part of the rack part is inserted in the spring 100, the other part of the rack part penetrates through the positioning block 62 and is connected with an adjusting gear 200 in a meshing manner, and the adjusting gear is hinged on the supporting rod 60.
Referring to fig. 3, a hinge groove 63 is formed at the upper end of the support rod 60, hinge shafts 64 are fixed on the front and rear side walls of the hinge groove, the middle part of the adjusting gear 200 is inserted and sleeved on the hinge shaft 64, one end of the adjusting gear 200 penetrates through the hinge groove 63 to be engaged with the rack part 81 of the support rod 80, the other end of the adjusting gear 200 penetrates through the hinge groove 63 and is fixed with a fan-shaped protective sleeve 300, and a convex toggle key 301 is formed on the side wall of the protective sleeve.
Referring to fig. 5, in the specific implementation of the hole puncher of the present invention, an operator holds the inclined hand lever 20 and stirs the adjusting gear 200 to rotate the adjusting gear 200, the adjusting gear 200 drives the rack portion 81 engaged therewith to move upward, the rack portion 81 drives the supporting rod 80 to move upward, the supporting rod 80 moves upward to drive the guide block 70 to move upward in the guide groove 61 of the supporting rod 60, the spring 100 is compressed, the supporting rod 80 drives the connecting rod 90 to move upward, the connecting rod 90 drives the arc-shaped lifting rod 40 hinged thereon to move upward, one end of the lifting rod 40 drives the bottom cover 30 at the bottom of the conical bucket 10 to rotate around the hinge with the conical bucket 10, in the process, the insertion groove 32 on the bottom cover 30 rotates around the guide ring 12 and compresses the return torsion spring 50, so as to open the bottom of the conical bucket 10, and then the conical bucket 10 is pressed up and down on a plate containing bacteria to punch the bacteria, after the punching is finished, the conical hopper body 10 is lifted upwards and the adjusting gear 200 is loosened, under the dual actions of the spring 100 and the reset torsion spring 50, the connecting rod 90 and the lifting rod 40 reset to drive the bottom cover 30 to reset and rotate around the hinged position of the conical hopper body 10, the reset torsion spring 50 resets to enable the insertion groove 32 to rotate on the guide ring 12 until the bottom cover 30 covers the bottom of the conical hopper body 10 again, so that fungus blocks cannot fall out of the conical hopper body 10, then the conical hopper body 10 is moved to the upper part of a fresh culture medium or gel, the conical hopper body 10 is inverted through the reverse inclined hand-held rod 20, and therefore the fungus blocks are moved into the fresh culture medium or gel.
In conclusion, the microbial strain puncher disclosed by the invention is convenient to punch and take out a bacterial block under the condition that a culture dish is not easy to pollute sundry bacteria, the bacterial block is not easy to slide off from the puncher during moving, and the bacterial block is easy to separate from the puncher during inoculation.
The microorganism culture punch provided by the present invention is only an embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art can easily think of changes or substitutions within the technical scope of the present invention, and all those persons skilled in the art should be covered by the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.