CN108727135A - A kind of special trichoderma of perennial fruit tree first biological organic fertilizer and its application entirely - Google Patents

A kind of special trichoderma of perennial fruit tree first biological organic fertilizer and its application entirely Download PDF

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CN108727135A
CN108727135A CN201810652361.2A CN201810652361A CN108727135A CN 108727135 A CN108727135 A CN 108727135A CN 201810652361 A CN201810652361 A CN 201810652361A CN 108727135 A CN108727135 A CN 108727135A
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trichoderma
organic fertilizer
fruit tree
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沈其荣
黄启为
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Nanjing Agricultural University
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C21/00—Methods of fertilising, sowing or planting
    • A01C21/005—Following a specific plan, e.g. pattern
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005—Cultivation methods
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/02—Cultivation of hops or vines
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    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80—Soil conditioners
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor

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  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
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Abstract

The invention discloses a kind of first biological organic fertilizer and its applications entirely of perennial special trichoderma of fruit tree.First biological organic fertilizer formula is+3% magnesium sulfate of+5% calcium nitrate of 71% trichoderma as biological organic fertilizer+5% urea+5% ammonium sulfate+6% potassium sulfate of+5% monoammonium phosphate to trichoderma entirely.Compared with the local common barnyard manure processing of application, using trichoderma, first biological organic fertilizer can dramatically increase the sugar content in fruit and significantly reduce the acid content in fruit entirely, it is 8-25% to increase sugared amplitude, deacidification amplitude is 20-50%, illustrate to pass through Rational Application trichoderma first biological organic fertilizer entirely, in terms of improving fruit quality and flavor there are larger potentiality can dig.

Description

A kind of special trichoderma of perennial fruit tree first biological organic fertilizer and its application entirely
Technical field
The invention belongs to organic fertilizer field, it is related to a kind of special trichoderma of perennial fruit tree and first biological organic fertilizer and its answers entirely With.
Background technology
Perennial fruit tree includes most of fruit fruit tree, citrus, grape, apple, cherry, loquat, longan, litchi Branch, wax-apple, Kiwi berry, dragon fruit and pears, peach, plum, apricot etc., the perennial fruit tree You50Duo Ge sections of culture and utility in China, More than 300 kind, cultivated area surpasses 1.3 hundred million mu.Since reform and opening-up, orchard workers obtain preferable economic benefit by volume increase. But past four during the decade, often excessive to apply chemical fertilizer since orchard workers excessively devote exclusive attention to output, especially excessive application chemistry Nitrogenous fertilizer causes all perennial orchard soil fertility all in deep fades, to be mainly manifested in orchard soil acidification seriously, even The apple orchard or theatre soil pH in the north drop to current 7 or so from 8 or so before 40 years, and what is had is even lower；Soil Hardened and topsoil shoals, and so that fruit tree root growth is obstructed, seriously affects absorption of the root system to nutrient, fruit yield biennial bearing is more next It is more serious；Weight chemical fertilizer, light organic fertilizer make fruit quality and flavor become worse and worse, and fruit market " fair average quality " supply exceed demand, It is even just rotten in orchard the edge of a field, and high-grade fruit is both rare expensive.Fruit industry arrived change, become quantity into The epoch of quality, then, government constantly put into effect the policy for promoting organic replacing fertilizer, organic fertilizer in the market or biological organic fertilizer Kind is dazzling, very different, and numerous orchard workers are even more blindly to apply or abuse organic fertilizer, also without receiving " May all your wish come true " Effect.
China carries out organic replacing fertilizer action on fruit tree at present, but without good organic fertilizer brand kind, makes this One action is difficult to receive effect.
Invention content
The purpose of the present invention is the above-mentioned deficiencies for the prior art, and it is raw to provide a kind of full member of the special trichoderma of perennial fruit tree Organic fertilizer.
It is a further object of the present invention to provide a kind of applications of the perennial special trichoderma of fruit tree first biological organic fertilizer entirely.
It is yet another object of the invention to provide a kind of applications of the perennial special trichoderma of fruit tree first biological organic fertilizer entirely.
The purpose of the present invention can be achieved through the following technical solutions：
A kind of special trichoderma of perennial fruit tree first biological organic fertilizer entirely, the full member biology of the special trichoderma of perennial fruit tree Organic fertilizer formula is：+ 5% urea+5%-14% ammonium sulfate+5%-9% monoammonium phosphate of 52%-71% trichoderma as biological organic fertilizer+ 6%-12% potassium sulfate+3%-5% calcium nitrate+3%-5% magnesium sulfate；And the sum of the mass percentage of all components is 100%.
First biological organic fertilizer formula is preferred entirely for the special trichoderma of perennial fruit tree：71% trichoderma as biological organic fertilizer+5% + 3% magnesium sulfate of+5% calcium nitrate of urea+5% ammonium sulfate+6% potassium sulfate of+5% monoammonium phosphate.
First biological organic fertilizer is preferably prepared via a method which to obtain the trichoderma entirely：
(1) solid fermentation is carried out to the Guizhou trichoderma NJAU4742 that culture presevation number is CGMCC NO.12166 and obtains trichoderma hair Ferment product；
(2) by acidolysis amino acid primary liquid and matured compost 1.5-3.0 in mass ratio:7.0-8.5 ratio be uniformly mixed, put down Weighing apparatus 5-10 days, object heap body pH value to be mixed is raised to 6.5 or more, then above-mentioned trichoderma solid fermentation product is pressed 50-300 kgs/tonne Amount be inoculated into mixture heap body, mixing, obtain matured compost and acidolysis amino acid containing trichoderma solid mixture, in temperature It spends under conditions of being no more than 60 DEG C and the water content of the solid mixture is evaporated to 30% hereinafter, obtaining trichoderma as biological organic fertilizer；
(3) on the basis of trichoderma as biological organic fertilizer, urea, ammonium sulfate, monoammonium phosphate, potassium sulfate, nitric acid are added thereto It three kinds or more in calcium and magnesium sulfate, mixes well, by the aqueous of the solid mixture under conditions of temperature is no more than 60 DEG C Amount is evaporated to 30% hereinafter, obtaining the special trichoderma of perennial fruit tree crop first bio-organic fertilizer entirely.
The preparation method of the special trichoderma of perennial fruit tree of the present invention first biological organic fertilizer entirely, includes the following steps：
(1) solid fermentation is carried out to the Guizhou trichoderma NJAU4742 that culture presevation number is CGMCC NO.12166 and obtains trichoderma hair Ferment product；
(2) by acidolysis amino acid primary liquid and matured compost 1.5-3.0 in mass ratio:7.0-8.5 ratio be uniformly mixed, put down Weighing apparatus 5-10 days, object heap body pH value to be mixed is raised to 6.5 or more, then above-mentioned trichoderma solid fermentation product is pressed 50-300 kgs/tonne Amount be inoculated into mixture heap body, mixing, obtain matured compost and acidolysis amino acid containing trichoderma solid mixture, in temperature It spends under conditions of being no more than 60 DEG C and the water content of the solid mixture is evaporated to 30% hereinafter, obtaining trichoderma as biological organic fertilizer；Its In, acidolysis amino acid primary liquid is prepared by the following method：(a) first by dead livestock and poultry animal through automation in closed container After broken, all solid content and liquid are transferred to closed hydrolytic decomposition pot automatically, in initial acid c (1/2H2SO4) it is 2.5-5mol L-1, hydrolyze 2-5 hours under 80-100 DEG C and 1-2 atmospheric pressure；(b) hydrolysis terminates to wait for that solution is cooled to 80 DEG C or less in hydrolytic decomposition pot When, stratification collects the amino acid solution in middle level up to acidolysis amino acid primary liquid；
(3) on the basis of trichoderma as biological organic fertilizer, urea, ammonium sulfate, monoammonium phosphate, potassium sulfate, nitric acid are added thereto It three kinds or more in calcium and magnesium sulfate, mixes well, by the aqueous of the solid mixture under conditions of temperature is no more than 60 DEG C Amount is evaporated to 30% hereinafter, obtaining the special trichoderma of perennial fruit tree crop first bio-organic fertilizer entirely.
The specific method that step (1) carries out Guizhou trichoderma NJAU4742 solid fermentation is preferred：
Function bacterium fermentation is prepared 1. Guizhou trichoderma NJAU4742 is inoculated into PDA liquid medium and is carried out liquid fermentation Liquid；
2. 10-20 times of crop material powder, acidolysis amino acid primary liquid dilution are pressed crop material powder：Acidolysis amino acid primary liquid 10-20 times of dilution=1Kg：The ratio mixing of 2-2.2L, ageing, pH value are adjusted to 3.0-5.0, obtain crop material and acidolysis ammonia Base acid blend, as solid fermentation culture medium；
3. Guizhou trichoderma NJAU4742 zymotic fluids are inoculated by 50-100L/ tons of amount in solid fermentation culture medium, PH3.0-5.0,28-30 DEG C of fermentation temperature, humidity 60-70%RH, tray culture obtain trichoderma solid fermentation product in 96-168 hours, Trichoderma solid fermentation product miospore quantity >=1 × 109Cfu/ grams.
The mass ratio of acidolysis amino acid primary liquid and matured compost preferably 1 in step (2):4.
Trichoderma as biological organic fertilizer described in step (2) contains 2 × 108It is the Guizhou trichoderma NJAU4742 of cfu/g or more, complete Nitrogen content mass ratio is 2.5-3.0%, and 90% or more mass ratio is organic nitrogen in full nitrogen, and total npk nutrient mass ratio is 5- 6%, content of organic matter quality >=45%, free amino acid and small peptide 2-3%, water content mass ratio≤30%, pH6-7.
The special trichoderma of perennial fruit tree described in step (3) contains 2 × 10 in first biological organic fertilizer entirely7Cfu/g or more Guizhou trichoderma NJAU4742, total nitrogen content mass ratio be 6.5% or more, 68% or more mass ratio is organic nitrogen in full nitrogen, always Npk nutrient mass ratio is 14% or more, 30% or more content of organic matter mass ratio, free amino acid and small peptide 1-2%, is contained Water mass ratio≤30%, pH6-7.
First biological organic fertilizer is promoting perennial fruit tree volume increase and is carrying the special trichoderma of perennial fruit tree of the present invention entirely Application in high-quality.
The perennial fruit tree preferably is selected from citrus, grape, cherry.
A method of improve perennial output of the fruit tree and quality, including per acre base apply 500 kilograms it is of the present invention more The special trichoderma of year fruit tree member biological organic fertilizer entirely, later stage normal fertilizer and water management.
The perennial fruit tree is selected from citrus, grape, cherry.
As the preferred of the method for the present invention, when the perennial fruit tree is selected from citrus, planting density is 100 plants/acre, cave Apply the special trichoderma of the perennial fruit tree member biological organic fertilizer 5kg fertilizer/strain entirely；Top dressing 4 times, the total dosage of top dressing are：Urea 90 kgs/acre, 20 kgs/acre of monoammonium phosphate, 80 kgs/acre of potassium sulfate；When the perennial fruit tree is selected from grape, plantation is close Degree is 300 plants/acre, the special trichoderma of the perennial fruit tree member biological organic fertilizer 1.7kg fertilizer/strain entirely of spreading manuer in holes；Top dressing 4 times, The total dosage of top dressing is：90 kgs/acre of urea, 20 kgs/acre of monoammonium phosphate, 80 kgs/acre of potassium sulfate；The perennial fruit tree When selected from cherry, planting density is 120 plants/acre, the special trichoderma of perennial fruit tree of spreading manuer in holes first biological organic fertilizer 4.2kg entirely Fertilizer/strain；Top dressing 4 times, the total dosage of top dressing are：90 kgs/acre of urea, 20 kgs/acre of monoammonium phosphate, 80 kgs/acre of potassium sulfate.
Advantageous effect：
The present invention provides a kind of all nutrition type trichoderma first biological organic fertilizer formula entirely, by inhibit orchard soil acidification, Regulate and control orchard soil microbiota, activation orchard soil bioactivity, realizes the mesh of the increasing yield and improving quality on a variety of fruit tree plants Mark.Compared with the common barnyard manure processing of application, using trichoderma entirely first biological organic fertilizer can dramatically increase in fruit containing sugar Acid content in amount and significant decrease fruit, it is 8-25% to increase sugared amplitude, and deacidification amplitude is 20-50%, illustrates to pass through and rationally apply With trichoderma first biological organic fertilizer entirely, in terms of improving fruit quality and flavor there are larger potentiality can dig.
Biological sample preservation information
NJAU4742, Classification And Nomenclature are Guizhou trichoderma Trichoderma guizhouense, are preserved in China Microbiological bacterium Kind preservation administration committee common micro-organisms center, the deposit date is on 04 11st, 2016, preservation address was Beijing's southern exposure The institute 3 of area North Star West Road 1, Institute of Microorganism, Academia Sinica, culture presevation number are CGMCC NO.12166.
Specific implementation mode
Embodiment 1
(1) microbial inoculum produces
(1) it by growth-promoting bacterial strain NJAU4742, is inoculated into PDA liquid medium and carries out liquid fermentation production, fermentation life The condition of production is：PH4.0,28 DEG C of fermentation temperature, under 175rpm (rev/min) rotating speed, continuous shake culture, growth time 6 Its (144hr.), as trichoderma zymotic fluid (contain bacterium or gemma amount >=1 × 1010cfu/ml)。
(2) by crop material powder 100g, 20 times of acidolysis amino acid primary liquid, 200 milliliters of dilution, mixing, ageing 10 hours, PH value is adjusted to 4.0, obtains the culture medium that crop material makes with acidolysis ispol.
Wherein acidolysis amino acid primary liquid is further preferably prepared by the following method：(1) first will in closed container Dead livestock and poultry animal is after automating broken, all solid contents and liquid is transferred to closed hydrolytic decomposition pot automatically, in initial acid c (1/2H2SO4) it is 3mol L-1, hydrolyze 2-5 hours under 100 DEG C and 1 atmospheric pressure；(2) hydrolysis terminates to wait for that solution is cold in hydrolytic decomposition pot When but to 80 DEG C or less, stratification collects the amino acid solution in middle level up to acidolysis amino acid primary liquid.
(3) NJAU4742 zymotic fluids crop material is inoculated by 100L/ tons of amount to make with acidolysis ispol Culture medium in, in pH5.0,28-30 DEG C of fermentation temperature, humidity 60-70%RH, tray (thickness≤3.5 centimetre) culture The trichoderma solid fermentation product of 144hr., tunning gemma amount >=1 × 109Cfu/ grams.
(2) trichoderma as biological organic fertilizer production
By acidolysis amino acid primary liquid and matured compost in mass ratio 2:8 ratio is uniformly mixed, and is balanced one week or so, is waited mixing It closes object heap body pH value and is raised to 6.5 or more.Above-mentioned NJAU4742 solid fermentation products are inoculated with by 100 kgs/tonne of amount again It into mixture heap body, mixes well, obtains the matured compost of the NJAU4742 containing trichoderma and the solid mixture of acidolysis amino acid. The water content of the solid mixture is evaporated to 30% hereinafter, as trichoderma as biological is organic under conditions of temperature is no more than 60 DEG C Fertilizer.
(3) complete first trichoderma as biological organic fertilizer production
On the basis of trichoderma as biological organic fertilizer, it is formulated according to table 1 and urea, ammonium sulfate, monoammonium phosphate, potassium sulfate, nitre is added Sour calcium and magnesium sulfate, mix well, and are evaporated to the water content of the solid mixture under conditions of temperature is no more than 60 DEG C 30% hereinafter, packaging manufacture is the special trichoderma of banana crops first bio-organic fertilizer entirely.
The 1 special trichoderma of perennial fruit tree of table first biological organic fertilizer formula (kg/tonne) entirely
The 2 special trichoderma of perennial fruit tree of table npk nutrient total content (%) in first biological organic fertilizer entirely
Formula N P2O5 K2O N+P2O5+K2O
1 5.33 3.14 2.84 11.3
2 6.22 3.69 4.19 14.09
3 7.20 4.55 5.05 16.8
4 7.90 5.40 6.09 20.2
5 5.80 3.02 2.72 11.53
6 6.69 3.57 4.07 14.32
7 7.57 4.43 4.93 16.93
8 8.37 5.28 6.78 20.43
The 3 special trichoderma of perennial fruit tree of table chemical fertilizer N-P-K content (%) in first biological organic fertilizer entirely
Formula N P2O5 K2O N+P2O5+K2O
1 2.66 1.8 1.5 5.96
2 3.85 2.5 3.0 9.35
3 5.10 3.5 4.0 12.6
4 6.10 4.50 6.00 16.6
5 3.37 1.8 1.5 6.67
6 4.56 2.5 3.0 10.06
7 5.71 3.5 4.0 13.21
8 6.81 4.5 6.0 17.31
Embodiment 2
The present embodiment is entirely first to investigate the perennial special trichoderma of fruit tree of the present invention by continuously planting citrus, grape, cherry The effect of biological organic fertilizer.
Planting density is:100 plants/acre of citrus (age of tree is 15 years when experiment), (age of tree is 9 to 300 plants/acre of grape when experiment Year), 120 plants/acre of cherry (when experiment the age of tree be 6 years), three planting fruit-trees first season results and the second season are much like, only list here Second season result.Production rate：It is calculated from each processing in examination cell (2 mu of area), picking 20 plants of fruits at random, after weighing flat Mean value, for every plant of average product of fertilizer formula processing；Meanwhile the random acquisition fruit in the 20 plants of fruits acquired, three Secondary repetition measures sugar content and acid content in fruit
Base manure amount of application：Barnyard manure or farmyard manure are 1500 kgs/acre, trichoderma first 500 kgs/acre of biological organic fertilizer entirely.
Except base manure applies kind in addition to quantity difference, the top dressing of all processing is identical, applies 4 times, and total dosage is：Urea 90 kgs/acre, 20 kgs/acre of monoammonium phosphate, 80 kgs/acre of potassium sulfate.
Test result (table 4) shows：On three seed pod tree plant of citrus, grape and cherry, 500 kilograms of trichodermas are applied per acre Full member biological organic fertilizer ratio makees base manure using 1.5 tons of local barnyard manure can more dramatically increase Tree Fruit yield, amount of increase in production citrus For 19-33%, grape 13-27%, cherry 18-43%, illustrate to apply 500 kilograms of trichoderma as biological organic fertilizer ratios per acre per acre Can also significantly it increase production using 1.5 tons of barnyard manure, Main Yield-increasing mechanism is to contain a large amount of trichoderma strains in trichoderma as biological organic fertilizer, is applied Enter and grow a large amount of reeseimycelials in fruit tree root system after soil, first, activation orchard's soil nutrient, two are to aid in perennial fruit tree Root system absorbs moisture and nutrient, third, reeseimycelial secretes a large amount of growth-promoting substance, promotes root growth, fourth, trichoderma mycelia Body can fast decoupled host plant root exudates and cast and overground part residuum, to eliminate the allelopathic of host plant Substance and abatement continuous cropping obstacle, it is ensured that the healthy growth of perennial fruit tree；In trichoderma first biological organic fertilizer (addition N P and K) entirely A small amount of calcium nitrate and magnesium sulfate are added in formula again, moreover it is possible to significantly improve fruit yield, increase rate citrus is 5-8%, grape For 4-6%, cherry 6-7%, illustrate the battalion necessary to the same place, plant due to perennial fruit bearing plant long term growth Foster element is often lacked, and the appropriate ca and mg fertilizer for supplementing plant wilderness demand can also make fruit yield increase by 5%.Trichoderma Full member biological organic fertilizer shows so high production-increasing function on perennial fruit tree plant, in addition to the beneficial work of reeseimycelial With outer, important function is also played containing abundant various plant essential nutrient elements in product.
4 base of table applies influence of different (biology) organic fertilizers to perennial output of the fruit tree and fruit parts quality index
(second season harvests after fertilising)
Table 4 the result shows that：Compared with the local common barnyard manure processing of application, using trichoderma, first biological organic fertilizer can entirely It dramatically increases the sugar content in fruit and significantly reduces the acid content in fruit, it is 8-25% to increase sugared amplitude, and deacidification amplitude is 20- 50%, illustrate through Rational Application trichoderma first biological organic fertilizer entirely, have in terms of improving fruit quality and flavor compared with Big potentiality can be dug.
In conclusion in tested formula, first biological organic fertilizer formula 6 can obtain fruit yield and compares trichoderma entirely High, sugar content of fruit highest and the minimum field efficacy of fruit acid content, the most preferably formula of the product are：71% trichoderma as biological + 3% magnesium sulfate of+5% calcium nitrate of organic fertilizer+5% urea+5% ammonium sulfate+6% potassium sulfate of+5% monoammonium phosphate.

Claims (10)

1. a kind of special trichoderma of perennial fruit tree first biological organic fertilizer entirely, it is characterised in that the special trichoderma of perennial fruit tree Member biological organic fertilizer formula is entirely：+ 5% urea+5%-14% ammonium sulfate+5%-9% phosphorus of 52%-71% trichoderma as biological organic fertilizer Sour monoammonium+6%-12% potassium sulfates+3%-5% calcium nitrate+3%-5% magnesium sulfate；And the mass percentage of all components it Be 100%.
2. the special trichoderma of perennial fruit tree according to claim 1 first biological organic fertilizer entirely, it is characterised in that described is more Member biological organic fertilizer formula is the special trichoderma of year fruit tree entirely：+ 5% ammonium sulfate+5% of+5% urea of 71% trichoderma as biological organic fertilizer + 3% magnesium sulfate of+5% calcium nitrate of+6% potassium sulfate of monoammonium phosphate.
3. the special trichoderma of perennial fruit tree according to claim 1 or 2 first biological organic fertilizer entirely, it is characterised in that described First biological organic fertilizer is prepared via a method which to obtain trichoderma entirely：
(1) solid fermentation is carried out to the Guizhou trichoderma NJAU4742 that culture presevation number is CGMCC NO.12166 and obtains trichoderma fermentation production Object；
(2) by acidolysis amino acid primary liquid and matured compost 1.5-3.0 in mass ratio:The ratio of 7.0-8.5 is uniformly mixed, and balances 5- 10 days, object heap body pH value to be mixed was raised to 6.5 or more, then above-mentioned trichoderma solid fermentation product is pressed to 50-300 kgs/tonne of amount Be inoculated into mixture heap body, mixing, obtain matured compost and acidolysis amino acid containing trichoderma solid mixture, temperature not The water content of the solid mixture is evaporated to 30% hereinafter, obtaining trichoderma as biological organic fertilizer under conditions of more than 60 DEG C；
(3) on the basis of trichoderma as biological organic fertilizer, thereto be added urea, ammonium sulfate, monoammonium phosphate, potassium sulfate, calcium nitrate and It three kinds or more in magnesium sulfate, mixes well, steams the water content of the solid mixture under conditions of temperature is no more than 60 DEG C 30% is sent to hereinafter, obtaining the special trichoderma of perennial fruit tree crop first bio-organic fertilizer entirely.
4. the preparation method of the special trichoderma of perennial fruit tree described in claim 1 first biological organic fertilizer entirely, it is characterised in that packet It includes：
(1) solid fermentation is carried out to the Guizhou trichoderma NJAU4742 that culture presevation number is CGMCC NO.12166 and obtains trichoderma fermentation production Object；
(2) by acidolysis amino acid primary liquid and matured compost 1.5-3.0 in mass ratio:The ratio of 7.0-8.5 is uniformly mixed, and balances 5- 10 days, object heap body pH value to be mixed was raised to 6.5 or more, then above-mentioned trichoderma solid fermentation product is pressed to 50-300 kgs/tonne of amount Be inoculated into mixture heap body, mixing, obtain matured compost and acidolysis amino acid containing trichoderma solid mixture, temperature not The water content of the solid mixture is evaporated to 30% hereinafter, obtaining trichoderma as biological organic fertilizer under conditions of more than 60 DEG C；Wherein, sour Solution amino acid primary liquid is prepared by the following method：(a) be first crushed dead livestock and poultry animal through automation in closed container, After all solid contents and liquid are transferred to closed hydrolytic decomposition pot automatically, in initial acid c (1/2H2SO4) it is 2.5-5mol L-1、 It is hydrolyzed 2-5 hours under 80-100 DEG C and 1-2 atmospheric pressure；(b) hydrolysis terminates when solution in hydrolytic decomposition pot is cooled to 80 DEG C or less, Stratification collects the amino acid solution in middle level up to acidolysis amino acid primary liquid；
(3) on the basis of trichoderma as biological organic fertilizer, thereto be added urea, ammonium sulfate, monoammonium phosphate, potassium sulfate, calcium nitrate and It three kinds or more in magnesium sulfate, mixes well, steams the water content of the solid mixture under conditions of temperature is no more than 60 DEG C 30% is sent to hereinafter, obtaining the special trichoderma of perennial fruit tree crop first bio-organic fertilizer entirely.
5. the preparation method of the special trichoderma of perennial fruit tree according to claim 4 first biological organic fertilizer entirely, feature exist It is to the specific method of Guizhou trichoderma NJAU4742 progress solid fermentations in step (1)：
1. Guizhou trichoderma NJAU4742 is inoculated into carry out liquid fermentation in PDA liquid medium and prepares fermented liquid by functional bacteria；
2. 10-20 times of crop material powder, acidolysis amino acid primary liquid dilution are pressed crop material powder：Acidolysis amino acid primary liquid 10- 20 times of dilution=1Kg：The ratio mixing of 2-2.2L, ageing, pH value are adjusted to 3.0-5.0, obtain crop material and acidolysis amino Acid blend, as solid fermentation culture medium；
3. Guizhou trichoderma NJAU4742 zymotic fluids are inoculated by 50-100L/ tons of amount in solid fermentation culture medium, in pH3.0- 5.0,28-30 DEG C of fermentation temperature, humidity 60-70%RH, tray culture 96-168 hours trichoderma solid fermentation product, trichoderma are solid Body tunning miospore quantity >=1 × 109Cfu/ grams.
6. the preparation method of the special trichoderma of perennial fruit tree according to claim 4 first biological organic fertilizer entirely, feature exist The mass ratio of acidolysis amino acid primary liquid and matured compost is 1 in step (2):4.
7. the preparation method of the special trichoderma of perennial fruit tree according to claim 4 first biological organic fertilizer entirely, feature exist Trichoderma as biological organic fertilizer described in step (2) contains 2 × 108Guizhou trichoderma NJAU4742, the total nitrogen content of cfu/g or more Mass ratio is 2.5-3.0%, and 90% or more mass ratio is organic nitrogen in full nitrogen, and total npk nutrient mass ratio is 5-6%, has Machine matter content quality >=45%, free amino acid and small peptide 2-3%, water content mass ratio≤30%, pH6-7；Institute in step (3) The special trichoderma of perennial fruit tree stated contains 2 × 10 in first biological organic fertilizer entirely7The Guizhou trichoderma NJAU4742 of cfu/g or more, Total nitrogen content mass ratio is 6.5% or more, and 68% or more mass ratio is organic nitrogen in full nitrogen, and total npk nutrient mass ratio is 14% or more, 30% or more content of organic matter mass ratio, free amino acid and small peptide 1-2%, water content mass ratio≤30%, pH6-7。
8. first biological organic fertilizer is promoting perennial fruit tree volume increase and is carrying the special trichoderma of perennial fruit tree described in claim 1 entirely Application in high-quality；The perennial fruit tree preferably is selected from citrus, grape, cherry.
9. a kind of method improving perennial output of the fruit tree and quality, it is characterised in that apply 500 kilograms of rights including base per acre and want Ask the special trichoderma of perennial fruit tree described in 1 first biological organic fertilizer entirely, later stage normal fertilizer and water management；The perennial fruit tree choosing From citrus, grape, cherry.
10. according to the method described in claim 9, it is characterized in that the perennial fruit tree be selected from citrus when, planting density It is 100 plants/acre, the special trichoderma of perennial fruit tree described in claim 1 of spreading manuer in holes member biological organic fertilizer 5kg fertilizer/strain entirely；Top dressing 4 times, the total dosage of top dressing is：90 kgs/acre of urea, 20 kgs/acre of monoammonium phosphate, 80 kgs/acre of potassium sulfate；Described is perennial When fruit tree is selected from grape, planting density is 300 plants/acre, and the full member of the special trichoderma of perennial fruit tree described in claim 1 of spreading manuer in holes is raw Organic fertilizer 1.7kg fertilizer/strain；Top dressing 4 times, the total dosage of top dressing are：90 kgs/acre of urea, 20 kgs/acre of monoammonium phosphate, sulphur 80 kgs/acre of sour potassium；When the perennial fruit tree is selected from cherry, planting density is 120 plants/acre, described in claim 1 of spreading manuer in holes The member biological organic fertilizer 4.2kg fertilizer/strain entirely of the special trichoderma of perennial fruit tree；Top dressing 4 times, the total dosage of top dressing are：Urea 90 is public Pound/acre, 20 kgs/acre of monoammonium phosphate, 80 kgs/acre of potassium sulfate.
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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109456118A (en) * 2019-01-09 2019-03-12 南京农业大学 A kind of orchard soil liquid inhibiting fruit tree tree body premature aging first biological organic fertilizer and its application entirely
CN110982711A (en) * 2019-12-04 2020-04-10 南京农业大学 Method for open production of trichoderma solid strain
CN111393206A (en) * 2020-03-31 2020-07-10 南京农业大学 Organic-inorganic full-element biological organic fertilizer containing composite bacteria and application thereof
CN114524694A (en) * 2022-02-22 2022-05-24 南京思农生物有机肥研究院有限公司 Special multifunctional biological organic fertilizer for sunshine muscat and preparation method and application thereof
CN114793827A (en) * 2022-05-24 2022-07-29 南京农业大学 Preparation method and application of fruit tree container seedling biological matrix

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CN107686406A (en) * 2017-09-20 2018-02-13 南京农业大学 A kind of special trichoderma of garlic first bio-organic fertilizer and its preparation method and application entirely
CN108129234A (en) * 2017-12-28 2018-06-08 南京农业大学 A kind of special complete first trichoderma as biological organic fertilizer for restoring old apple tree productivity and its preparation method and application

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CN107686406A (en) * 2017-09-20 2018-02-13 南京农业大学 A kind of special trichoderma of garlic first bio-organic fertilizer and its preparation method and application entirely
CN108129234A (en) * 2017-12-28 2018-06-08 南京农业大学 A kind of special complete first trichoderma as biological organic fertilizer for restoring old apple tree productivity and its preparation method and application

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109456118A (en) * 2019-01-09 2019-03-12 南京农业大学 A kind of orchard soil liquid inhibiting fruit tree tree body premature aging first biological organic fertilizer and its application entirely
CN110982711A (en) * 2019-12-04 2020-04-10 南京农业大学 Method for open production of trichoderma solid strain
CN110982711B (en) * 2019-12-04 2020-08-07 南京农业大学 Method for open production of trichoderma solid strain
CN111393206A (en) * 2020-03-31 2020-07-10 南京农业大学 Organic-inorganic full-element biological organic fertilizer containing composite bacteria and application thereof
CN114524694A (en) * 2022-02-22 2022-05-24 南京思农生物有机肥研究院有限公司 Special multifunctional biological organic fertilizer for sunshine muscat and preparation method and application thereof
CN114793827A (en) * 2022-05-24 2022-07-29 南京农业大学 Preparation method and application of fruit tree container seedling biological matrix

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Application publication date: 20181102