CN108709884A - Method for preparing glycosylated nano-gold by one-step method and application thereof - Google Patents
Method for preparing glycosylated nano-gold by one-step method and application thereof Download PDFInfo
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- CN108709884A CN108709884A CN201810504753.4A CN201810504753A CN108709884A CN 108709884 A CN108709884 A CN 108709884A CN 201810504753 A CN201810504753 A CN 201810504753A CN 108709884 A CN108709884 A CN 108709884A
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- 238000000034 method Methods 0.000 title claims abstract description 34
- 239000010931 gold Substances 0.000 title claims description 24
- 229910052737 gold Inorganic materials 0.000 title claims description 19
- 230000013595 glycosylation Effects 0.000 claims abstract description 46
- 238000006206 glycosylation reaction Methods 0.000 claims abstract description 46
- 238000006243 chemical reaction Methods 0.000 claims abstract description 24
- FCBUKWWQSZQDDI-UHFFFAOYSA-N rhamnolipid Chemical compound CCCCCCCC(CC(O)=O)OC(=O)CC(CCCCCCC)OC1OC(C)C(O)C(O)C1OC1C(O)C(O)C(O)C(C)O1 FCBUKWWQSZQDDI-UHFFFAOYSA-N 0.000 claims abstract description 24
- 101710186708 Agglutinin Proteins 0.000 claims abstract description 16
- 101710146024 Horcolin Proteins 0.000 claims abstract description 16
- 101710189395 Lectin Proteins 0.000 claims abstract description 16
- 101710179758 Mannose-specific lectin Proteins 0.000 claims abstract description 16
- 101710150763 Mannose-specific lectin 1 Proteins 0.000 claims abstract description 16
- 101710150745 Mannose-specific lectin 2 Proteins 0.000 claims abstract description 16
- 239000000910 agglutinin Substances 0.000 claims abstract description 16
- UIIMBOGNXHQVGW-UHFFFAOYSA-M sodium bicarbonate Substances [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims abstract description 12
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims abstract description 12
- 229910021642 ultra pure water Inorganic materials 0.000 claims abstract description 11
- 239000012498 ultrapure water Substances 0.000 claims abstract description 11
- 229910004042 HAuCl4 Inorganic materials 0.000 claims abstract description 9
- HBOMLICNUCNMMY-KJFJCRTCSA-N 1-[(4s,5s)-4-azido-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione Chemical compound O=C1NC(=O)C(C)=CN1C1O[C@H](CO)[C@@H](N=[N+]=[N-])C1 HBOMLICNUCNMMY-KJFJCRTCSA-N 0.000 claims abstract description 6
- 239000002245 particle Substances 0.000 claims description 25
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 18
- 238000002360 preparation method Methods 0.000 claims description 5
- 239000013049 sediment Substances 0.000 claims description 5
- HVCOBJNICQPDBP-UHFFFAOYSA-N 3-[3-[3,5-dihydroxy-6-methyl-4-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyoxan-2-yl]oxydecanoyloxy]decanoic acid;hydrate Chemical compound O.OC1C(OC(CC(=O)OC(CCCCCCC)CC(O)=O)CCCCCCC)OC(C)C(O)C1OC1C(O)C(O)C(O)C(C)O1 HVCOBJNICQPDBP-UHFFFAOYSA-N 0.000 claims description 4
- 229930186217 Glycolipid Natural products 0.000 claims description 3
- SJUCACGNNJFHLB-UHFFFAOYSA-N O=C1N[ClH](=O)NC2=C1NC(=O)N2 Chemical class O=C1N[ClH](=O)NC2=C1NC(=O)N2 SJUCACGNNJFHLB-UHFFFAOYSA-N 0.000 claims description 3
- 238000005253 cladding Methods 0.000 claims description 3
- 230000036571 hydration Effects 0.000 claims description 3
- 238000006703 hydration reaction Methods 0.000 claims description 3
- 239000002077 nanosphere Substances 0.000 claims description 3
- 230000035484 reaction time Effects 0.000 claims description 3
- 238000011895 specific detection Methods 0.000 claims description 3
- 230000001279 glycosylating effect Effects 0.000 claims description 2
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 claims 1
- 235000010520 Canavalia ensiformis Nutrition 0.000 claims 1
- 235000010518 Canavalia gladiata Nutrition 0.000 claims 1
- 102000002322 Egg Proteins Human genes 0.000 claims 1
- 108010000912 Egg Proteins Proteins 0.000 claims 1
- 240000001723 Entada phaseoloides Species 0.000 claims 1
- 235000014103 egg white Nutrition 0.000 claims 1
- 210000000969 egg white Anatomy 0.000 claims 1
- 238000001514 detection method Methods 0.000 abstract description 6
- 239000000203 mixture Substances 0.000 abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 3
- 239000002244 precipitate Substances 0.000 abstract 2
- 238000001816 cooling Methods 0.000 abstract 1
- 239000000047 product Substances 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 32
- 239000002105 nanoparticle Substances 0.000 description 12
- 230000015572 biosynthetic process Effects 0.000 description 10
- 238000003786 synthesis reaction Methods 0.000 description 9
- 239000003638 chemical reducing agent Substances 0.000 description 8
- 238000010521 absorption reaction Methods 0.000 description 7
- 239000012141 concentrate Substances 0.000 description 6
- 238000007792 addition Methods 0.000 description 5
- 150000001720 carbohydrates Chemical class 0.000 description 5
- 235000014633 carbohydrates Nutrition 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 239000003446 ligand Substances 0.000 description 4
- 239000003223 protective agent Substances 0.000 description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- 108010039491 Ricin Proteins 0.000 description 3
- 229940098773 bovine serum albumin Drugs 0.000 description 3
- 239000007853 buffer solution Substances 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- 238000005457 optimization Methods 0.000 description 3
- 238000000862 absorption spectrum Methods 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 239000003876 biosurfactant Substances 0.000 description 2
- 230000003197 catalytic effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 125000003147 glycosyl group Chemical group 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000002086 nanomaterial Substances 0.000 description 2
- 239000000376 reactant Substances 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000005054 agglomeration Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 150000001335 aliphatic alkanes Chemical group 0.000 description 1
- 239000012496 blank sample Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000007306 functionalization reaction Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 239000011241 protective layer Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000001308 synthesis method Methods 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/3103—Atomic absorption analysis
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- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Saccharide Compounds (AREA)
Abstract
The invention discloses a method for preparing glycosylated nanogold by a one-step method and application thereof, which comprises the steps of dissolving rhamnolipid into ultrapure water, uniformly mixing, and adjusting pH to 6-7 to obtain rhamnolipid solution; adding HAuCl4·3H2O is added to NaHCO3The method comprises the steps of adding a rhamnolipid solution with the final concentration of 1-4 mmol/L into a solution, uniformly mixing the rhamnolipid solution with the final concentration of 1-3 g/L, placing the mixture into a water bath kettle at the temperature of 20-80 ℃ for reaction for 0.1-4 h, cooling a reaction solution to room temperature, centrifuging the reaction solution, collecting precipitates, and dissolving the precipitates into ultrapure water to obtain a product, wherein the glycosylation nanometer hardware has the performance of visually detecting agglutinin and the like, and the agglutinin is concanavalin with the minimum detection concentration of 30 nM.
Description
Technical field
The present invention relates to it is a kind of using rhanolipid as biosurfactant one-step synthesis method glycosylate nanogold method,
Belong to field of nanometer material technology.
Background technology
Gold nanoparticle has become people and studies one of widest nano material, and gold nanoparticle is most stable of gold
Belong to nano-particle, since preparation method is simple and its distinctive Physicochemical property in surface, for example, catalytic property, electrical properties,
Magnetic and optical characteristics.It has been had a wide range of applications in the fields such as catalytic field and biomedicine.
When illumination is mapped to particle surface, local surface plasma resonance can be generated on its surface（SPR）Effect, and
This effect is very sensitive to the medium and environment of surrounding.Based on this SPR characteristics, when in system carbohydrate with its by
When body interacts, gold nanoparticle can be caused largely to assemble, when grain spacing is from decreasing below 2.5 times of particle diameter
When, the color of suspension can become purple from red, and this variation can be usually seen by the naked eye.Kataoka groups are to grind at first
Study carefully agglutinin and glycosylate the seminar of nano-complex interaction, they have found that agglutinin alternative identifies glycosyl type
And cause particle buildup, so as to cause its uv-visible absorption spectra red shift and broaden.Therefore, this unique optical
Matter can make glycosylation nanogold as high sensitive sensor.
Now there is several methods that being used to synthesis glycosylation gold nano-material.First method is made with reducing sugar
For reducing agent and ligand, during gold nanoparticle is formed, reducing agent of the carbohydrate as golden presoma passes through hydroxyl
Base and metallographic interaction, make it form protective layer on gold nanoparticle surface.Another method is local reduction way, by sulfydryl work(
The carbohydrate of energyization is added in the precursor solution of gold, and when gold nanoparticle is formed, carbohydrate is just made
For the surface ligand of gold nanoparticle.Ligand exchange method is also the method for preparing saccharification gold nanoparticle, and gold nanoparticle is first
It is produced, the original ligand of particle surface is replaced by the carbohydrate of sulfhydrylation.Previous synthesis glycosylates nano-particle
Method it is usually very complicated, surface glycosyl Limited Number, and need to functionalization sugar carry out multi step modification modification, more purification mistake
Journey, flow are complicated.
Therefore, there is an urgent need in the art to develop a kind of novel synthetic method preparation glycosylation nano-particle, one can be passed through
Function glycan molecule is efficiently connected on nanogold particle by kind simple effective method, to improve the detection of nano-Au composite
Efficiency.
Invention content
The object of the present invention is to provide a kind of glycosylation nanogold is prepared using rhamnolipid as reducing agent one-step method
Method, rhamnolipid had not only been reducing agent but also had been protective agent, present device, operation letter during preparing glycosylation nanogold
Single, reaction condition is mild.Prepared glycosylation nanogold has the function of visualizing specific detection agglutinin.
The purpose of the present invention is what is be achieved through the following technical solutions：
Rhamnolipid, is first dissolved into ultra-pure water, is adjusted to pH 6-7, mix by a kind of method that one-step method prepares glycosylation nanogold
It closes uniform；Prepare 5-50 mM NaHCO3Weak solution is for use；By HAuCl4·3H2O is added to NaHCO3In solution, final concentration
For 1-4 mmol/L, then rhamnolipid solution is added, final concentration of 1 ~ 3 g/L is uniformly mixed；It is positioned over 20 DEG C -80 DEG C
0.1-4 h are reacted in water-bath.Reaction solution is cooled to room temperature, is centrifuged, sediment is collected and is dissolved in ultra-pure water to get product.
In the present invention, rhamnolipid is a kind of biosurfactant of glycolipid class, by hydrophilic rhamnopyranosyl and is dredged
Aqueous alkane chain composition；Rhamnolipid had not only been reducing agent but also had been protective agent, because rhamnolipid is first made compared with indissoluble solution
Solution adds in reaction, and initial concentration is limited without specific, preferably highly concentrated solution, such as is made into the rhamnolipid of 50 g/L
Solution.
It, can be by HAuCl in the present invention4·3H2O is directly added into NaHCO3In solution, make its final concentration of 1-4 mmol/
L, can also be first by HAuCl4·3H2O is configured to solution and adds in reaction, HAuCl4·3H2The concentration of O solution is preferably 2.5
mol/L。
The mass ratio of the rhamnolipid and three hydration tetra chlorauric acids is 1: 0.1-1:2.
The reaction temperature is more preferably 50 DEG C ~ 80 DEG C.
The mass ratio of the rhamnolipid and three hydration tetra chlorauric acids is more preferably 1:0.4-1:0.8.
The reaction time is more preferably 20 min-1 h.
5 ~ 15 nm of particle size average out to.
The glycosylation nanogold particle has one or more features selected from the group below：
(1) average grain diameter of glycosylation nanogold particle is about 5 ~ 15 nm；
(2) in glycosylating nanogold particle, the grain size of 80% or more nanogold particle is within the scope of 2-30 nm.
(3) glycosylation nanogold particle is the gold nanosphere of rhamnopyranosyl cladding.
(4) there is larger distance between the glycosylation nanogold particle formed, keep good dispersibility.
Obtained glycosylation nanogold has the function of to visualize specific detection agglutinin etc..
Above-mentioned agglutinin is concanavalin（ConA）.
Above-mentioned glycosylation nanogold has the function of that the specific agglutinin of detection, the minimal detectable concentration of concanavalin are
30 nM。
Advantageous effect：
The present invention directly obtains that shape is regular using rhamnolipid as reducing agent, the smooth glycosylation nanogold in surface.The party
It is method mild condition, easily-controllable, it is equipment, easy to operate.Rhamnolipid is both reducing agent and protective agent, reduces the group of reaction system
Point.The glycosylation nanogold of synthesis has the performance for detecting specific agglutinin, may be implemented using the effect of this specific recognition
Detection, capture and separation etc. to bacterium.
Description of the drawings
Fig. 1 is the transmission electron microscope for the glycosylation nanogold that preparation method of the present invention obtains.
Fig. 2 is the full wavelength scanner of different time synthesis glycosylation nanogold.
Fig. 3 is the full wavelength scanner of synthesis glycosylation nanogold under different temperatures.
Fig. 4 is the real scene shooting figure of synthesis glycosylation nanogold under different temperatures.
Fig. 5 is the full wavelength scanner of various concentration gold particle synthesis glycosylation nanogold.
Fig. 6 is the real scene shooting figure of various concentration gold particle synthesis glycosylation nanogold.
Fig. 7 is the full wavelength scanner in the ConA of addition various concentration.
Fig. 8 is the real scene shooting figure in the ConA of addition various concentration.
Fig. 9 is ConA, the BSA of addition with concentration（Bovine serum albumin(BSA)）And RCA120（Ricin (WA) agglutinin）All-wave
Long scan.
Figure 10 is ConA, the BSA of addition with concentration（Bovine serum albumin(BSA)）And RCA120（Ricin (WA) agglutinin）Real scene shooting
Figure.
Specific implementation mode
Embodiment 1
A kind of method that one-step method prepares glycosylation nanogold, includes the following steps：
1）First rhamnolipid is dissolved into ultra-pure water, is made into the solution of 50 g/L, is adjusted to pH 6-7, is uniformly mixed；
2）By NaHCO3It is dissolved in ultra-pure water, it is for use to be made into 10 mM weak solutions；
3）By 2.5 mol/L HAuCl4·3H2O solution is added to NaHCO3In solution, final concentration of 3 mmol/L, then by mouse
Lee's glycolipid sample is added, final concentration of 2.5 g/L, is uniformly mixed；It is positioned in 80 DEG C of water-baths and reacts 1 h.By 5 min,
10 min, 20 min, 40 min, 60 min, 120 min samplings.Reaction solution is cooled to room temperature, centrifugation, is collected sediment and is dissolved in
To get product in ultra-pure water, Fig. 2 is seen.As seen from the figure, in 60 min as time increases, maximum absorption band is constant always
For 531 nm, illustrate in the increase of certain time, product does not interfere with the size of nanogold in continuous accumulation.Reaction 1
It is OD that maximum absorption peak, which increases, after h531=0.8247.In 120 min, apparent red shift has occurred in spectrogram, illustrates anti-
After extending to a certain extent between seasonable, agglomeration may occur for product.Therefore it is the peak optimization reaction time to have chosen 1 h.Institute
For the transmission electron microscope for the glycosylation nanogold being prepared as shown in Figure 1, shape is regular, surface is smooth, 5 ~ 15 nm of average grain diameter
Grain, glycosylation nanogold particle are the gold nanosphere of rhamnopyranosyl cladding, are had between the glycosylation nanogold particle of formation larger
Distance, keep good dispersibility.
Embodiment 2
First rhamnolipid is dissolved into pure water, is made into the concentrate of 50 g/L, is adjusted to pH 6-7, is uniformly mixed；Prepare 20 mM
NaHCO3Weak solution is for use；By HAuCl4·3H2O is added to NaHCO3In solution, final concentration of 2 mmol/L, then by sandlwood
Glycolipid concentrate is added, final concentration of 1.5 g/L, is uniformly mixed；Be respectively placed in 37 DEG C, 60 DEG C, it is anti-in 80 DEG C of water-baths
Answer 1 h.Reaction solution is cooled to room temperature, centrifugation, is collected sediment and is dissolved in ultra-pure water to get product, sees Fig. 3,4.As seen from the figure,
When reaction temperature is 80 DEG C, the maximum absorption peak of reactant is maximum.It can be seen that as temperature increases, reaction rate is maximum, production
Measure highest.Therefore 80 DEG C of peak optimization reaction temperature is had chosen.It can be made that shape is regular, and surface is smooth, average grain diameter 5 ~ 15 nm sugar
Base nanogold particle.
Embodiment 3
First rhamnolipid is dissolved into pure water, is made into the concentrate of 50 g/L, is adjusted to pH 6-7, is uniformly mixed；Prepare 10 mM
NaHCO3Weak solution is for use；By HAuCl4·3H2O concentrates are added separately to NaHCO3In solution, its final concentration is set to be respectively
1.5,2.5,3 and 3.5 mmol/L, then rhamnolipid concentrate is added, final concentration of 2 g/L, it is uniformly mixed；It is positioned over
1 h is reacted in 80 DEG C of water-baths.Reaction solution is cooled to room temperature, centrifugation, is collected sediment and is dissolved in ultra-pure water to get product, sees
Fig. 5,6.As seen from the figure, the HAuCl of various concentration is added4·3H2The maximum absorption band of O, product are different.In a certain concentration
Under range, HAuCl4·3H2The concentration of O additions is higher, and the maximum absorption peak of reactant is bigger.Therefore HAuCl is had chosen4·
3H2O adds the peak optimization reaction concentration of a concentration of 3.5 mmol/L.It can be made that shape is regular, and surface is smooth, average grain diameter 5 ~ 15
Nm glycosylates nanogold particle.
Embodiment 4
Product obtained, the biosensors as detection agglutinin will be reacted in embodiment 1.By concanavalin（ConA）
It is configured to concentrate with PBS buffer solution, is added in the glycosylation nano-Au solution obtained of embodiment 1, final concentration is respectively 30
NM, 100 nM, 150 nM, 200 nM and 250 nM.In addition it is added in the PBS buffer solution to glycosylation nano-Au solution of equivalent,
As blank control group.The result of 6 h sample detection all-wave lengths is shown in Fig. 7,8.As seen from the figure, after long-time stands reaction, most
Big absorption peak reduces obviously, and spectrogram tends towards stability, and can visually be clearly visible purple particulate deposits.Meanwhile compound concentration is
10 μM of bovine serum albumin(BSA)（BSA）Solution, ricin (WA) agglutinin（RCA120）Solution with concentration concanavalin
（ConA）Solution is added in glycosylation nano-Au solution, is added in addition in the PBS buffer solution to glycosylation nano-Au solution of equivalent,
As blank control group.Its specific selectivity to agglutinin is detected after standing and reacting, sees Fig. 9,10.As seen from the figure, same
Under concentration conditions, reacts glycosylation nanogold obtained and BSA and RCA is being added120All-wave length curve weighs substantially with blank sample afterwards
It closes, absorption spectrum is there is no generation movement and broadens, and maintains the original optical property of particle, reaction system is also without apparent
Variation；And spectrum has occurred apparent red shift and broadens after ConA is added, maximum absorption band reduces obviously, and spectrogram becomes gentle,
And visible generation purple particulate deposits of naked eyes in the reaction system.Illustrate the method for the present invention glycosylation nanogold obtained to ConA
There is specific selectivity.
Above-described embodiment the method equipment is simple, easy to operate, and reaction condition is mild.Rhamnolipid be both reducing agent again
It is protective agent, reduces the component of reaction system.The glycosylation nanometer gold surface of synthesis is smooth, as shown in Figure 1.
Finally illustrate, the above embodiments are merely illustrative of the technical solutions of the present invention, rather than protects model to the present invention
The limitation enclosed.Any equivalent improvement to the present invention, should all be within the scope of the present invention.
Claims (9)
1. a kind of method that one-step method prepares glycosylation nanogold, which is characterized in that preparation process is as follows：
1）, rhamnolipid is dissolved into ultra-pure water, be uniformly mixed, adjust pH 6-7, obtain rhamnolipid solution；
2）, by HAuCl4·3H2O is added to NaHCO3In solution, final concentration of 1-4 mmol/L, then by rhamnolipid solution
It is added, final concentration of 1 ~ 3 g/L, is uniformly mixed；It is positioned in 20 DEG C of -80 DEG C of water-baths and reacts 0.1-4 h；
3）, by step（2）In reaction solution be cooled to room temperature, centrifuge, collect sediment be dissolved in ultra-pure water to get product.
2. the method that the one-step method according to claim 1 prepares glycosylation nanogold, which is characterized in that step 2）Middle institute
State NaHCO3Solution concentration is 5-50 mM.
3. the method that one-step method according to claim 1 prepares glycosylation nanogold, which is characterized in that step 2）Middle sandlwood
The mass ratio of glycolipid and three hydration tetra chlorauric acids is 1: 0.1-1:2.
4. the method that one-step method according to claim 1 prepares glycosylation nanogold, which is characterized in that step 2）Middle reaction
Temperature is preferably 40 DEG C ~ 80 DEG C.
5. the method that one-step method according to claim 1 prepares glycosylation nanogold, which is characterized in that step 2）Middle reaction
Time is preferably the h of 10 min ~ 1.
6. the glycosylation nanogold that any one of the claim 1-5 preparation methods are prepared.
7. glycosylation nanogold according to claim 6, which is characterized in that the glycosylation nanogold particle has choosing
From the one or more features of the following group：
(1) average grain diameter of glycosylation nanogold particle is 5 ~ 15 nm；
(2) in glycosylating nanogold particle, the grain size of 80% or more nanogold particle is within the scope of 2-30 nm;
(3) glycosylation nanogold particle is the gold nanosphere of rhamnopyranosyl cladding;
(4) there is larger distance between the glycosylation nanogold particle formed, keep good dispersibility.
8. application of the glycosylation nanogold in specific detection agglutinin described in claim 7.
9. application according to claim 8, which is characterized in that the agglutinin is concanavalin, with sword bean ball egg
White minimal detectable concentration is 30 nM.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114208847A (en) * | 2021-12-30 | 2022-03-22 | 泗阳糖宝新材料科技有限公司 | Core-shell structure glycosylated nano-silver antibacterial material and green preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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