CN108690025A - Utilize the method for active ingredient in acidic oxidation and alkaline reduction electric potential water extraction kuh-seng - Google Patents
Utilize the method for active ingredient in acidic oxidation and alkaline reduction electric potential water extraction kuh-seng Download PDFInfo
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- CN108690025A CN108690025A CN201710234353.1A CN201710234353A CN108690025A CN 108690025 A CN108690025 A CN 108690025A CN 201710234353 A CN201710234353 A CN 201710234353A CN 108690025 A CN108690025 A CN 108690025A
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- C07—ORGANIC CHEMISTRY
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- C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
- C07D471/22—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed systems contains four or more hetero rings
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Abstract
The present invention relates to a kind of methods using active ingredient in acidic oxidation and alkaline reduction electric potential water extraction kuh-seng, it is that kuh-seng is extracted with acidic oxidized electric potential water, or to extract kuh-seng with acidic oxidized electric potential water after alcoholic solution immersion wetting kuh-seng, extracting solution is condensed into medicinal extract, it is alkalized to pH value 7.5~8.8 with alkaline reduction electric potential water, stratification collects upper solution and crystallizes to obtain matrine.Extracting solution is either obtained with alcoholic solution extraction kuh-seng, acidic oxidized electric potential water is added and is adjusted to pH value≤3, isolates solution, then pH value 7.5~8.8 is adjusted to alkaline reduction electric potential water, stratification collects upper solution, and crystallization obtains matrine.The method of the present invention can not use any organic solvent, and low cost efficiently extracts kuh-seng alkaloid.
Description
Technical field
The invention belongs to biomedicine technical field, prepared by the extraction for being related to active ingredient in medicinal plant, especially relate to
And the method using acidic oxidation and alkaline reduction the electric potential water effective component extracting from medicinal plant kuh-seng.
Background technology
Kuh-seng (Sophora flavescensAlt) it is a kind of common Chinese herbal medicine, has description more in history tree works,
Primary efficacy is clearing heat and detoxicating, dispel pathogenic wind and remove dampness, desinsection is antipruritic.Kuh-seng is distributed throughout the country, resourceful.Pharmacological evaluation and
Clinical application proves, kuh-seng and its preparation can treat acute bacillary dysentery, trichomoniasis, bronchial asthma and asthma type brochitis,
Oedema and ascites, skin disease, arrhythmia cordis and vital myocarditis, hypnosis and mania etc..
Contained alkaloid composition is considered as the main biological activity of kuh-seng in kuh-seng.Containing there are many kuh-sengs in kuh-seng
Alkaloid, at present from kuh-seng separation identify 17 kinds of alkaloid compositions, mainly include sophocarpine (Sophocarpine),
Matrine (matrine), Sophoridine (sophoridine), oxymatrine (oxymatrine) and N-Oxysophocarpine
(oxysophcarpine), this five kinds of alkaloids account for 90% of total alkaloid content or more.Wherein matrine, oxymatrine
All have the function of clearing heat and detoxicating, promoting blood circulation, swelling and pain relieving and anticancer and activity, cure mainly tumour, hepatitis, Ascites Complicating Liver Cirrhosis,
The signs such as lung abscess, especially to lung cancer, gastric cancer, the cancer of the esophagus, cervical carcinoma the effects that it is preferable.Especially oxymatrine can treat
Leukopenia, chronic hepatitis B and hepatitis caused by leucocyte caused by tumor radiotherapy, chemotherapy is low and other reasons.It is clinical
In application, requiring the purity of oxymatrine bulk pharmaceutical chemicals 98% or more.Sophoridine has active anticancer to many animals transplantable tumor,
Clinic obviously stablizes suede cancer chorioadenoma, lung cancer, digestive system cancer antitumaous effect, is that a kind of of great value anticancer is new
Medicine.Since kuh-seng alkaloid all shows a variety of apparent activity, domestic and international pharmacy in terms of pharmacological research and clinical application
Worker attaches great importance to the research of kuh-seng alkaloid chemical composition.
Traditional kuh-seng alkaloid extraction process mainly include water cooking and extraction method, solvent extraction method, ion exchange extraction method,
Resin adsorption extraction method, supercritical fluid extraction method etc., wherein the most universal with solvent extraction method.Solvent extraction method using water,
As extraction solvent, extracting method is mostly dipping, diacolation, decoction, reflux etc. for acid and ethyl alcohol etc..Kuh-seng alkaloid tradition is extracted
The major defect of technique is that extraction flow is complicated, and production efficiency is not high, especially the wherein extraction of oxymatrine and Sophoridine
Program is more complicated, and production cost is high, it is difficult to form industrial-scale production;Followed by impurity content is more in extract, no
Conducive to purifying, active ingredient purity is low, it is difficult to reach 98% or more;In addition, in traditional extraction technique kuh-seng biology total alkali purifying
To be extracted and be stripped with organic solvent, for plant extraction organic-inorganic solvent or have certain toxicity, or to plant
Object extract component has pollution, and extraction process also has environment serious pollution, is not easy to the extensive green ecological environment of low cost
Production.The above problem greatly hampers the application of matrine, oxymatrine, Sophoridine in pharmacy and clinically.
Invention content
The purpose of the present invention is overcoming the shortcomings of current medicinal plant kuh-seng extraction process, provides and a kind of utilizing acidic oxidation
The method for extracting active ingredient in kuh-seng with alkaline reduction electric potential water with low cost, efficiently extracts kuh-seng alkaloid.
Method of the present invention using active ingredient in acidic oxidation and alkaline reduction electric potential water extraction kuh-seng is with pH
The acidic oxidized electric potential water of value 2~5 extracts kuh-seng, and extracting solution is condensed into medicinal extract, then with the alkaline reduction electric potential water of pH value 9~13
Alkalization to pH value 7.5~8.8, stratification collects upper solution, and crystallization obtains matrine.
Further, the present invention extracts the method packet of active ingredient in kuh-seng using acidic oxidation and alkaline reduction electric potential water
It includes:
1) Lightyellow Sophora Root is impregnated to abundant wetting with the acidic oxidized electric potential water of pH value 2~5, is warming up to 50~100 DEG C of progress water
It carries, collects extracting solution, be condensed into medicinal extract;
2) medicinal extract is alkalized with the alkaline reduction electric potential water of pH value 9~13 to pH value 7.5~8.8, stratification, collects upper layer
Solution, crystallization obtain matrine crude product.
Further, described for water to carry the acidic oxidized electric potential water of kuh-seng it is preferable to use the acidic oxidations of pH value 2~3
Electric potential water;The alkaline reduction electric potential water is preferably the alkaline reduction electric potential water of pH value 11~13.
In the above method of the present invention, the time that the Lightyellow Sophora Root impregnates wetting in acidic oxidized electric potential water is preferably 5
~48 hours.
In turn, present invention preferably employs gradation extraction method, Lightyellow Sophora Root is repeatedly extracted with acidic oxidized electric potential water.
Usually, the extraction time is 3~5 times.Meanwhile in multiple extraction process, the total water in ensureing the aqueous extraction step
On the basis of amount is 4~20 times of kuh-seng weight, the water consumption that each water carries is identical or gradually reduces.
The extracting solution that the water raises is condensed into the medicinal extract that proportion at 20~80 DEG C is not less than 1.1 by the present invention, preferably
It is condensed into the medicinal extract of proportion 1.10~1.62.It is highly preferred that the present invention concentrates the extracting solution using double effect evaporator.
In the above method of the present invention, the dosage of the alkaline reduction electric potential water for the medicinal extract that alkalizes is generally medicinal extract quality
1~2 times.
The present invention is recrystallized matrine crude product obtained above using conventional method repeatedly, finally obtains matrine
Product.
In turn, of the present invention to extract the method for active ingredient in kuh-seng also using acidic oxidation and alkaline reduction electric potential water
Can be that the acidic oxidized electric potential water of pH value 2~5 is impregnated wetting kuh-seng to make into impregnate wetting kuh-seng with alcoholic solution, then to immersion
The acidic oxidized electric potential water of pH value 2~5 is added in the kuh-seng of wetting, water extracts kuh-seng and is condensed into medicinal extract according to the method described above,
Stratification after being alkalized with alkaline reduction electric potential water is collected upper solution and crystallizes to obtain matrine.
Wherein, the alcoholic solution can be pure methanol or ethanol solution, can also be that volumetric concentration is not less than 10%
The aqueous solution of methanol or ethyl alcohol.
Further, the present invention also provides another extracts kuh-seng using acidic oxidation and alkaline reduction electric potential water
The method of middle active ingredient is directly to obtain extracting solution with alcoholic solution extraction kuh-seng, and the acid of pH value 2~3 is added in extracting solution concentration
Property electrolyzed oxidizing water is adjusted to pH value≤3, isolates solution, then be adjusted to pH value with the alkaline reduction electric potential water of pH value 9~13
7.5~8.8, stratification collects upper solution, and crystallization obtains matrine crude product.
The further process of the third method of the above-mentioned offer of the present invention is:
1) kuh-seng is added in alcoholic solution, is extracted at 10~50 DEG C, extracting solution is concentrated to give extraction concentrate;
2) pH value≤3, stratification are acidified to by concentrate is extracted with the acidic oxidized electric potential water of pH value 2~3, collection contains
The aqueous solution of alkaloid;
3) will be water-soluble basified to pH value 7.5~8.8 containing alkaloid with the alkaline reduction electric potential water of pH value 9~13, it stands
Layering, collects upper solution, and crystallization obtains matrine crude product.
Further, the acidification step described in the third of the invention method can also be divided into the progress of two steps, i.e., first to carrying
Addition partially acidic electrolyzed oxidizing water in concentrate is taken, adjusts pH value to≤4, stratification divides isolated impurity substance,
Collect solution layer;The pH value for being collected into solution is continued to be adjusted to≤3 with acidic oxidized electric potential water, stratification is collected again
Solution layer obtains the aqueous solution containing alkaloid.
This is because when acidic oxidized electric potential water being added into the extraction concentrate of kuh-seng, the pH value of concentrate just starts
Decline quickly, subsequent decrease speed can be gradually slack-off.If acidic oxidized electric potential water is added at one time, it is likely to result in
Part is acid too strong in concentrate, and acidic oxidized electric potential water reacts uneven with kuh-seng, keeps product quality not easy to control.Therefore,
First partially acidic electrolyzed oxidizing water is added into extraction concentrate in the present invention, allow one section of the quiet leaching of kuh-seng and acidic oxidized electric potential water when
Between, after being sufficiently mixed uniform reaction, it is further continued for that acidic oxidized electric potential water is added, the pH value for adjusting concentrate continues to decline, directly
To≤3.
In the third method of the invention, the mass volume ratio for extracting used kuh-seng raw material and alcoholic solution is 1: 4~10.
Equally, the third method of the invention can also use gradation extraction method, be carried out to kuh-seng raw material with alcoholic solution multiple
Extraction.
Then, the present invention is to obtain the ratio of 0.5~2ml concentrates according to 1g kuh-seng raw materials, and the extracting solution is concentrated
At extraction concentrate.
As second method, the alcoholic solution used in the third method of the invention can be that pure methanol or ethyl alcohol are molten
Liquid can also be the aqueous solution of methanol of the volumetric concentration not less than 10% or ethyl alcohol.
It is molten with the upper layer that the alkalization of alkaline reduction electric potential water and stratification obtain in three kinds of above-mentioned extracting methods of the present invention
Liquid can not also be crystallized directly, but after being evaporated, then with alkaline reduction current potential water dissolution, and condensing crystallizing obtains again after filtering
Matrine crude product.
In turn, it is to utilize above-mentioned three kinds of extracting method alkali the present invention also provides a kind of production method of oxymatrine
Hydrogen peroxide is added in the upper solution changed and obtained after stratification in the upper solution, and 10~50 DEG C of oxidations are complete, concentration
Crystallization obtains oxymatrine crude product.
Equally, obtained oxymatrine crude product is recrystallized repeatedly, Oxymatrine alkali product can be finally obtained.
Hydrogen peroxide used in the above method is the aqueous hydrogen peroxide solution of mass concentration 5~30%.
Preferably, in any one of the above extracting method of the present invention, used kuh-seng raw material is 10~120 purpose of granularity
Kuh-seng raw material.
The present invention be directed to current kuh-seng alkaloid extraction process deficiency, provide it is a kind of using acidic oxidation and alkalinity also
The method that former current potential water extracts active ingredient in kuh-seng.Extracting method operation cost of the present invention is low, extraction efficiency is high, nothing in product
Residual solvent, to environment almost non-pollution discharge.
The primary raw material acidic oxidized electric potential water and alkaline reduction electric potential water used in the present invention is by natural water or originally
Water ionizes under electric field action, can make by oneself or directly buy.The acidic oxidized electric potential water or alkaline reduction electric potential water
PH value is determined by the size of extra electric field, direction, time and NaCl additive amounts.In atmospheric environment, acidic oxidized electric potential water
Or the pH value of alkaline reduction electric potential water can constantly change, alkalinity or acid degree constantly reduce, and gradually neutralize and tend to be neutral.This
The acidic oxidized electric potential water or alkaline reduction electric potential water of invention self-control or purchase are sealed by vacuum insulation vessels to be transported, and ensures its pH
Value is stablized within a certain period of time.
It is Banlangen that the present invention extracts the mechanism of action of kuh-seng alkaloid using acidic oxidation and alkaline reduction electric potential water
Class compound is soluble in acid solution, such as acidic oxidized electric potential water mostly.Kuh-seng wetting is soaked in acidic oxidized electric potential water, it is bitter
Kuh-seng alkaloid in ginseng plant is combined to form unstable salt with acidic oxidized electric potential water, then with alkaline reduction current potential scale
Change reduction, unstable kuh-seng alkaloid salt is converted to kuh-seng alkaloid Precipitation, and Banlangen class compound is in alkaline item
Open loop under part, while acidic oxidized electric potential water can neutralize mutually with alkaline reduction electric potential water and become neutral.Therefore, present invention profit
Banlangen class compound is precipitated with acidic oxidation and alkaline reduction electric potential water, by the pH value and extraction temperature that adjust electric potential water
The optimization of degree, the high efficiency to reach Banlangen are precipitated, this is the great innovation of plant extraction principle in the world.
It extracts and is stripped, technical process toxicity repeatedly using organic solvent in traditional kuh-seng biology total alkali extraction process
Greatly, production operation is not safe enough.Present invention process method does not use organic solvent, and extraction process is easy to operate, and it is dirty to reduce environment
Dye, and effective component extraction rate is high, and purification purity is high, can effectively improve production efficiency, reduces production cost, is suitble to extensive
Industrialized production.
Specific implementation mode
Technical solution of the present invention is explained in detail below by way of specific embodiment.It should be noted that below only
It is the illustration using acidic oxidation and alkaline reduction electric potential water extraction kuh-seng alkaloid.Those skilled in the art can design
Go out many changes and optional composition, method and system, basic principle method and range without being detached from the present invention.Cause
This, appended claims are intended to covering and relate to the use of acidic oxidized electric potential water and alkaline reduction electric potential water extraction kuh-seng active ingredient
Production technology and method.Although having carried out detailed citing description below the present invention, the embodiment is also only of the invention
One of some details of embodiment.Core of the invention content is not being departed from:Acidic oxidized electric potential water and alkaline reduction current potential
Under the premise of water extraction process is conceived, various modifications and improvements can be made, these should all be belonged to the scope of protection of the present invention.
Embodiment 1
Medicinal plant kuh-seng is carried out to conventional cleaning in stainless steel sink, taking-up is dried.It is again that clean kuh-seng is dry and crush
At 100 targeted fine powder of granularity.
It takes 10kg kuh-seng fine powders to be laid in saturation tank, the acidic oxidized electric potential water of 80L pH value 3 is added, by kuh-seng fine powder
All infiltrations are impregnated 24 hours.
The kuh-seng fine powder of wetting is fitted into stainless steel multi-function extractor, 80 DEG C is heated to and extracts 2 hours, filter, receive
Collect extracting solution, 40L acidic oxidized electric potential waters are added in the dregs of a decoction and are extracted 1 hour, extracting solution is collected, adds 20L acidic oxidations
Electric potential water extracts 1 hour.Merge No. 3 extracting solutions, material residue discards.
Extracting solution after merging is condensed into the medicinal extract of proportion 1.32 at 30 DEG C in double effect evaporator.2 are added in medicinal extract
The alkaline reduction electric potential water of the pH value 12 of times medicinal extract quality is stirred to react uniformly.The acid oxidation potential of a small amount of pH value 3 is added
Water, thin-layer chromatography check that the spot of wherein oxymatrine and N-Oxysophocarpine disappears.
Medicinal extract after above-mentioned alkalization is staticly settled, alkaloid is made all to be transferred to water layer, detaches water-yielding stratum.
Water layer containing alkaloid is continued to be alkalized to pH value 7.5, stratification with the alkaline reduction electric potential water of pH value 12,
Lower aqueous solution is discarded, cooling is stood after the solution heating concentration that kuh-seng alkaloid is contained on upper layer, crystallization is precipitated, hardship is obtained by filtration
Join alkali crude product.
The mother liquor filtered out is distributed chromatography through 7 grades or more reflux, can also obtain matrine crude product and Sophoridine is thick
Product.
Merge gained matrine crude product, recrystallizes repeatedly, obtain kuh-seng alkali product.It is fixed to obtain Chinese scholartree through recrystallization for Sophoridine crude product
Alkali product.
Embodiment 2
The kuh-seng raw material of 80 mesh of 10kg granularities is taken, is added in the ethanol solution of 50L volumetric concentrations 30%, 50 DEG C is warming up to, with frequency
The supersonic oscillations of 200KHz extract 30min.Extraction fluid is condensed into 10L.
The acidic oxidized electric potential water of 50L pH value 2.5 is added in concentrate, stirs evenly, stratification, impurity is precipitated
After substance, the solution containing kuh-seng alkaloid is isolated.
Continue the pH value with the acidic oxidized electric potential water adjusting solution of pH value 2.5 to 3, stratification collects solution again
Layer, obtains the aqueous solution containing alkaloid.
The pH value of aqueous solution is adjusted to 7.5 with the alkaline reduction electric potential water of pH value 12, it is water-soluble to discard lower layer for stratification
Liquid, the upper solution heating concentration isolated are evaporated, then with the alkaline reduction current potential water dissolution of pH value 12, filtering, filtrate concentration.
25% aqueous hydrogen peroxide solution is added in concentrate, the oxidation reaction 30min at 30 DEG C, until hydrogen peroxide consumption is complete,
Solution is evaporated, and obtains oxymatrine crude product;It will be recrystallized after obtained oxymatrine crude product acetone solution, then with acetone
Recrystallization is primary, obtains Oxymatrine alkali product.
Claims (18)
1. a kind of method using active ingredient in acidic oxidation and alkaline reduction electric potential water extraction kuh-seng is with pH value 2~5
Acidic oxidized electric potential water extracts kuh-seng, and extracting solution is condensed into medicinal extract, then is alkalized to pH with the alkaline reduction electric potential water of pH value 9~13
Value 7.5~8.8, stratification collect upper solution, and crystallization obtains matrine.
2. according to the method described in claim 1, it is characterized in that the method includes:
1) Lightyellow Sophora Root is impregnated to abundant wetting with the acidic oxidized electric potential water of pH value 2~5, is warming up to 50~100 DEG C of progress water
It carries, collects extracting solution, be condensed into medicinal extract;
2) medicinal extract is alkalized with the alkaline reduction electric potential water of pH value 9~13 to pH value 7.5~8.8, stratification, collects upper layer
Solution, crystallization obtain matrine crude product.
3. method according to claim 1 or 2, it is characterized in that the pH value of the acidic oxidized electric potential water is 2~3, alkalinity
The pH value of reduction potential water is 11~13.
4. according to the method described in claim 2, it is characterized in that the Lightyellow Sophora Root to be impregnated to profit in acidic oxidized electric potential water
Wet 5~48 hours.
5. method according to claim 1 or 2, it is characterized in that using gradation extraction method, with acidic oxidized electric potential water to hardship
Ginseng is repeatedly extracted.
6. according to the method described in claim 5, it is characterized in that the extraction time is 3~5 times.
7. according to the method described in claim 6, it is characterized in that total dosage of the acidic oxidized electric potential water is the 4 of kuh-seng weight
~20 times, the water consumption that each water carries is identical or gradually reduces.
8. method according to claim 1 or 2, it is characterized in that the extracting solution that the water raises is condensed into 20~80 DEG C
Lower proportion is not less than 1.1 medicinal extract.
9. method according to claim 1 or 2, it is characterized in that the use of the alkaline reduction electric potential water for the medicinal extract that alkalizes
Amount is 1~2 times of medicinal extract quality.
10. a kind of method using active ingredient in acidic oxidation and alkaline reduction electric potential water extraction kuh-seng is soaked with alcoholic solution
After bubble wetting kuh-seng, the acidic oxidized electric potential water that pH value 2~5 is added extracts kuh-seng, and extracting solution is condensed into medicinal extract, then with pH value 9
~13 alkaline reduction electric potential water alkalizes to pH value 7.5~8.8, stratification, collects upper solution, and crystallization obtains matrine.
11. a kind of method using active ingredient in acidic oxidation and alkaline reduction electric potential water extraction kuh-seng is carried with alcoholic solution
Kuh-seng is taken to obtain extracting solution, extracting solution concentration, the acidic oxidized electric potential water that pH value 2~3 is added is adjusted to pH value≤3, isolates
Solution, then pH value 7.5~8.8 is adjusted to the alkaline reduction electric potential water of pH value 9~13, stratification collects upper solution, knot
Crystalline substance obtains matrine.
12. the method according to claim 11, it is characterized in that the method includes:
1) kuh-seng is added in alcoholic solution, is extracted at 10~50 DEG C, extracting solution is concentrated to give extraction concentrate;
2) pH value≤3, stratification are acidified to by concentrate is extracted with the acidic oxidized electric potential water of pH value 2~3, collection contains
The aqueous solution of alkaloid;
3) will be water-soluble basified to pH value 7.5~8.8 containing alkaloid with the alkaline reduction electric potential water of pH value 9~13, it stands
Layering, collects upper solution, and crystallization obtains matrine.
13. method according to claim 11 or 12, it is characterized in that first adjusting extraction concentrate with acidic oxidized electric potential water
PH value to≤4, stratification collects solution layer, then is adjusted to pH value≤3 with acidic oxidized electric potential water, and stratification is simultaneously point
Separate out solution.
14. method according to claim 11 or 12, it is characterized in that the kuh-seng raw material and alcoholic solution that use of extracting
Mass volume ratio is 1: 4~10.
15. method according to claim 11 or 12, it is characterized in that obtaining the ratio of 0.5~2ml concentrates according to 1g kuh-sengs
Example, the extracting solution is concentrated.
16. the method according to claim 10 or 11, it is characterized in that the alcoholic solution is pure methanol or ethanol solution,
The either aqueous solution of methanol of the volumetric concentration not less than 10% or ethyl alcohol.
17. it is to utilize institute using the method for the kuh-seng aqueous slkali production oxymatrine that claim 1,10 or 11 methods produce
The upper solution obtained after alkalization and stratification to be stated, hydrogen peroxide is added in the upper solution, 10~50 DEG C of oxidations are complete,
Condensing crystallizing obtains oxymatrine.
18. according to the method for claim 17, it is characterized in that the mass concentration of the hydrogen peroxide is 5~30%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109908035A (en) * | 2019-04-25 | 2019-06-21 | 山西纳安生物科技有限公司 | A kind of freckle-removing mask based on peony seed oil |
| CN115671203A (en) * | 2022-11-24 | 2023-02-03 | 广州中医药大学科技产业园有限公司 | Traditional Chinese medicine compound extract for reducing blood sugar and blood fat and extraction method thereof |
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| CN1793142A (en) * | 2005-12-27 | 2006-06-28 | 中国科学院山西煤炭化学研究所 | Process for producing high purity matrine oxide by kuh-seng |
| CN101735644A (en) * | 2009-11-20 | 2010-06-16 | 苏州大学 | Method for extracting ipomoes batatas L. anthocyanidins |
| CN102633798A (en) * | 2012-04-26 | 2012-08-15 | 宁夏紫荆花制药有限公司 | Method for preparing high-purity matrine from sophora alopecuroides |
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| CN1453276A (en) * | 2003-05-22 | 2003-11-05 | 王答祺 | Prepn of matrine, oxymatrine and sophoxidine from flavescent sophora root |
| CN1793142A (en) * | 2005-12-27 | 2006-06-28 | 中国科学院山西煤炭化学研究所 | Process for producing high purity matrine oxide by kuh-seng |
| CN101735644A (en) * | 2009-11-20 | 2010-06-16 | 苏州大学 | Method for extracting ipomoes batatas L. anthocyanidins |
| CN102633798A (en) * | 2012-04-26 | 2012-08-15 | 宁夏紫荆花制药有限公司 | Method for preparing high-purity matrine from sophora alopecuroides |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109908035A (en) * | 2019-04-25 | 2019-06-21 | 山西纳安生物科技有限公司 | A kind of freckle-removing mask based on peony seed oil |
| CN115671203A (en) * | 2022-11-24 | 2023-02-03 | 广州中医药大学科技产业园有限公司 | Traditional Chinese medicine compound extract for reducing blood sugar and blood fat and extraction method thereof |
| CN115671203B (en) * | 2022-11-24 | 2023-10-27 | 广州中医药大学科技产业园有限公司 | A Chinese medicinal compound extract for lowering blood sugar and blood lipid, and its extraction method |
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