CN108684614A - The device and application method for preventing feed mouldy in a kind of Frankliniella occidentalis feeding process - Google Patents

The device and application method for preventing feed mouldy in a kind of Frankliniella occidentalis feeding process Download PDF

Info

Publication number
CN108684614A
CN108684614A CN201810388977.3A CN201810388977A CN108684614A CN 108684614 A CN108684614 A CN 108684614A CN 201810388977 A CN201810388977 A CN 201810388977A CN 108684614 A CN108684614 A CN 108684614A
Authority
CN
China
Prior art keywords
kidney bean
frankliniella occidentalis
frame
mouldy
feeding process
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201810388977.3A
Other languages
Chinese (zh)
Other versions
CN108684614B (en
Inventor
张晓明
胡昌雄
段盼
陈国华
李正跃
桂富荣
张宏瑞
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yunnan Agricultural University
Original Assignee
Yunnan Agricultural University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yunnan Agricultural University filed Critical Yunnan Agricultural University
Priority to CN201810388977.3A priority Critical patent/CN108684614B/en
Publication of CN108684614A publication Critical patent/CN108684614A/en
Application granted granted Critical
Publication of CN108684614B publication Critical patent/CN108684614B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/90Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463Organic compounds; Microorganisms; Enzymes
    • A23L3/3481Organic compounds containing oxygen
    • A23L3/3508Organic compounds containing oxygen containing carboxyl groups

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Environmental Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physiology (AREA)
  • Insects & Arthropods (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Biotechnology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Molecular Biology (AREA)
  • Birds (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nutrition Science (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The present invention relates to the device for preventing feed mouldy in a kind of Frankliniella occidentalis feeding process and application methods, the device includes the box body (1) that a top is set as open groove profile, and handle (5) is provided on the lateral wall of box body (1);It is equipped with fine sand (3) in box body (1), filter paper (4) is equipped on fine sand (3), kidney bean frame (2) is set on filter paper (4).The present invention has the function of easy to operate, reasonable design, saves manual labor, improves breeding efficiency, when studying the prevention problem of China's Frankliniella occidentalis, largely indoor population can be promoted the use energy fast-propagation for experiment in each R&D institution and colleges and universities.

Description

The device and application method for preventing feed mouldy in a kind of Frankliniella occidentalis feeding process
Technical field
The invention belongs to technical field of insect feeding, and in particular to prevent feed in a kind of Frankliniella occidentalis feeding process:Four Season the mouldy apparatus structure and application method technical field of beans.
Background technology
Xi Huajima [Frankliniella occidentalis(Pergande)]Belong to the one of Thysanoptera flower thrips category The gardening plants pests such as the important global vegetables of kind, flowers can cause blade, fruit rust brown spot occur, make fruit Real commodity is lost, other than directly endangering host plant, it is also possible to propagate tomato spotted wilf virus disease and garden balsam necrotic spot disease Poison, domestic and international expert are always so far still in the direction of effort for the study on prevention of Frankliniella occidentalis.Number occurs for Frankliniella occidentalis Amount is big, and harm time is long, and the problem of serious and safety prevents occurs for Frankliniella occidentalis present on large area production.Carry out A large amount of laboratory test studies the prevention problem of Frankliniella occidentalis, lays the foundation for control Frankliniella occidentalis harm is done step-by-step.
Mass rearing Frankliniella occidentalis population is needed in the laboratory experiment of Frankliniella occidentalis, kidney bean is as in Frankliniella occidentalis room The common feedstuffs of raising have the characteristics that cheap, plantation is simple, it is easy to obtain, in the prior art, for Frankliniella occidentalis Using kidney bean raise place kidney bean it is mouldy method it is also less, and indoors in feeding process kidney bean it occur frequently that hair Mould situation, seriously affects the existence of Frankliniella occidentalis, and it is excessively cumbersome to replace feed, and thrips is made to escape, when the placement of kidney bean Between cannot reach different time and be put into and replace and lose the nymph of thrips, cause thrips largely to escape and dead, it is difficult to indoors Breed a large amount of thrips population.It is existing that Frankliniella occidentalis is cultivated using kidney bean, it is not only increased in feeding process artificial Cost and the amount of labour, operating process needs larger labor intensity and time loss, and cannot effectively fast-propagation go out greatly Frankliniella occidentalis population is measured, the feeding efficiency of thrips is reduced.Therefore, a kind of easy to operate, simple in structure, raising appearance how is designed Easily, reproductive efficiency height and the high mouldy method of feeds such as kidney bean for preventing cultivation Frankliniella occidentalis of survival rate become this field section Grind the direction of personnel's effort.
Invention content
For the present invention precisely in order to the defect that solves the above problems, providing in a kind of Frankliniella occidentalis feeding process prevents feed mouldy Device and application method.
The present invention adopts the following technical scheme that realization.
The device for preventing feed mouldy in a kind of Frankliniella occidentalis feeding process, the present invention device include that a top is arranged For the box body of open groove profile, handle is provided on the lateral wall of box body;It is equipped with fine sand in box body, is equipped on fine sand Kidney bean frame is arranged in filter paper on filter paper;The fine sand thickness is set as 1cm-3cm;The kidney bean frame includes two pieces Inverted concave plate B before and after side inverted concave plate A and two pieces, four blocks of inverted concave plates fasten to form one piece of rectangular channel box, in the rectangular channel It is equipped with stainless steel cloth plate in box, forms the kidney bean frame of a rectangle;The quantity of kidney bean frame is set as N number of, when N >=3 When, the box body bottom of groove profile is provided with 2, remaining kidney bean frame is in folded pagoda pattern 2 kidney bean framves arranged at the bottom On;The area of the filter paper of the laying is greater than or equal to the area that kidney bean frame projects downward vertically.Using this kidney bean Kidney bean can be placed on kidney bean frame by apparatus for placing achievees the effect that ventilation, and the utilization of sand table is placed conveniently within foster worm It is moistening in cage, in sand table can be provide certain humidity in insect cage, ensure thrips survival.
It is further that folded pagoda pattern of the present invention is, the side inverted concave plate A of the kidney bean frame and front and back The side of inverted concave plate B is both provided with the vertical interpolation slit being mutually matched and vertical cutting, and vertical insert is inserted into the vertical cutting To fix kidney bean frame in seam;The kidney bean frame bottom of last layer and next layer of kidney bean top of the trellis are also provided with and are mutually matched Horizontal interpolation slit and horizontal cutting, the vertical cutting be inserted into vertical interpolation slit to fix the kidney bean frame of combination.
It is further that of the present invention to be equipped with stainless steel cloth plate in the rectangular channel box, structure is, in side The inner wall middle and upper part of inverted concave plate A and front and back inverted concave plate B are provided with the support horizontal stripe of a circle continuously or discontinuously, and described is stainless Expanded sheet metal is arranged on the support horizontal stripe;Or, being provided with several small hooks on four sides of stainless steel wire web plate, work as stainless steel wire When web plate is filled in rectangular channel box, small hook catches on the top edge of four wall of rectangular channel box to support stainless steel wire web plate.
It is further that filter paper of the present invention is circular filter paper.
It is further that four pieces of notch plate materials of kidney bean frame of the present invention are wooden;It is sized to:Side undercut Shape plate A, specification are long 15cm, high 8cm, deep 4cm, the wide 4cm of concave side;Front and back inverted concave plate B, specification are long 8cm, high 8cm, depth 4cm, the wide 2cm of concave side, it is 2-6 mesh to have stainless steel cloth expanded metals mesh number in kidney bean frame, and web plate position is far from top top opening Distance is 2cm.Kidney bean adds to solve using stainless steel is chronically at rotten in high humidity environment ask with wooden materials Topic, while without using irony frame because it gets rusty easily, both the above material is all unfavorable for extending the service life of this device, stainless Steelframe, which has, makes simple, stable structure, will not corrosion the characteristics of;Kidney bean frame solves too small and excessive four using this specification Season, beans can fall the problem of being difficult to place, and bottom is excessive in kidney bean frame height, places unstable and thrips nymph is difficult to If the loss of borer population is difficult to return on Kidney bean and occurred on filter paper.
Use the method for the device for preventing feed mouldy in a kind of above-mentioned Frankliniella occidentalis feeding process, this method packet of the present invention It includes and is set to the device in the insect cage of raising Frankliniella occidentalis, insect cage is placed in insectary or in growth cabinet; When the device is placed on insectary, which is, temperature is 26~28 DEG C, raising humidity is 60%~ 70%;When the device to be placed in growth cabinet, growth cabinet condition setting is that temperature is 26~28 DEG C, illumination Shading value L:D=16:8, raising humidity is 70%~80%.Be conducive to the preservation of Kidney bean under this humiture and more beneficial be The existence of Frankliniella occidentalis, humidity is excessively high to make the too fast drying of Kidney bean, the excessively high growth and development for being unfavorable for Frankliniella occidentalis of humidity.
It is further that this method of the present invention includes the chemical pretreatment of kidney bean, chemical pretreatment includes the following steps, It is fresh-keeping mould proof according to the component configuration of sodium benzoate 0.1-0.3g, potassium sorbate 5g, citric acid 15g, sodium citrate 15g, water 1L Agent, after kidney bean is peeled off throw impregnated in the fresh-keeping mould inhibitor of configuration after five minutes take out dry it is spare.
Be further that this method of the present invention includes the seed selection of kidney bean, that is, select resistance is stronger, after pesticide treatments It is not easy mouldy U.S.'s provider's kind or the kind of Nanjing ideal seedling Co., Ltd supply.
Be further that this method of the present invention includes the implantation methods of kidney bean, in the greenhouse will after presprouting of seeds, plant in In greenhouse in nylon gauze, insect infestations are prevented;During experiment, a water is poured within every 2~3 days, until being picked when kidney bean maturation It is spare;Any pesticide is not sprayed during experiment.
It is further that this method of the present invention includes following sequential steps, step 1:Fresh-keeping mould inhibitor is configured, in electronic scale On weigh sodium benzoate 0.2g, potassium sorbate 5g, citric acid 15g sodium citrate 15g, with purification water dissolution after be diluted to 1L, fill Enter spare in reagent bottle;
Step 2:The fine sand for cleaning drying is put in box body, fine sand thickness is 1-3cm, by the fresh-keeping anti-of above-mentioned configuration Mould dose is poured into fine sand, and agent liquid of being subject to does not agglomerate spilling on fine sand surface, after spread and filter paper and placed at least two four seasons Whole device is placed into Frankliniella occidentalis insect cage by beans frame;The fine sand of moistening can be that Frankliniella occidentalis supports worm to a certain extent Cage provides humidity, is covered with circular filter paper, a diameter of 15cm of filter paper, for specification relatively conventional in laboratory, this design thereon There is following benefit, on the one hand can prevent from growing on filter paper and fine sand containing above-mentioned fresh-keeping mould inhibitor in the fine sand of moistening mould Bacterium, another aspect filter paper be white, if having when thrips falls to bottom to observe in time, and filter paper block after can prevent Only thrips contacts with sand and loses polypide;
Step 3:Impregnate in above-mentioned fresh-keeping mould inhibitor to take out after five minutes after kidney bean is peeled off and dry, after be put into it is foster On a holder in worm cage, the quantity for placing kidney bean is suitably adjusted according to insect cage size, is at least covered entire Frame upper;It impregnates to allow fresh-keeping mould inhibitor to be effectively attached to kidney bean surface, raising is carried out after drying can prevent Kidney bean table Surface humidity is excessive to cause Frankliniella occidentalis dead;
Step 4:The kidney bean after fresh-keeping mould inhibitor impregnates is put after 5-8 days on another holder, is placed in first time Kidney bean observes the kidney bean on holder after 8-10 days, at this time under this environmental condition, the kidney bean on holder has been dried, and And it is not mouldy, will be dried and determine cleans out insect cage without thrips in kidney bean above;And so on four on each holder Season, beans were divided into 8-10 days between replacing, and the time difference on two holders is 5-6 days.
Beneficial effects of the present invention are:Prevent the feeds such as kidney bean mouldy in a kind of Frankliniella occidentalis feeding process of the present invention Method, using the above structure with fresh-keeping mould inhibitor;The box body can hold fine sand in the present invention, cover filter paper, and thin Sand is moistening, solves in traditional cultural method and to be directly not added with caused by water moistening in insect cage that humidity is too low using fine sand Thrips is dead, and covering filter paper solves polypide and directly falls on fine sand is easy to damage polypide on fine sand, population be difficult to expand it is numerous, carefully Fresh-keeping mould inhibitor is added in sand and solves causes to infect since the device in the environment such as fine sand and filter paper is mouldy to raising west flower Ji On the kidney bean of horse so that there is a large amount of mouldy sources in feeding environment, be added in fine sand and on filter paper fresh-keeping mould inhibitor this It is not related in traditional cultural method;Kidney bean is placed on dedicated kidney bean frame, can effectively ensure kidney bean It is ventilated, and have a certain distance with bottom fine sand, can prevent kidney bean directly contacted with fine sand it is airtight caused by it is mouldy, At least two kidney bean framves are placed in box body can reach the time difference for replacing kidney bean;Fresh-keeping mould inhibitor is used in the present invention Use prevent the kidney bean of raising Frankliniella occidentalis mouldy and seriously affect the existence of Frankliniella occidentalis, preferred fresh-keeping mould inhibitor There are use, fractions also to use frequently as preservative in food, test in the present invention in a variety of places and environment It was found that the growth and development of Frankliniella occidentalis and survive and do not make significant difference, and kidney bean raises Frankliniella occidentalis being put into insect cage It is preceding processed with fresh-keeping mould inhibitor, it can effectively prevent the kidney bean in feeding process from inventing the problem for leading to thrips death, carry The high numerous efficiency of expansion of Frankliniella occidentalis.The present invention has easy to operate, reasonable design, saves manual labor, improves cultivation effect The effect of rate, when studying the prevention problem of China's Frankliniella occidentalis, largely indoor population, can be each for experiment for energy fast-propagation A R&D institution and colleges and universities are promoted the use.
The present invention is further explained with reference to the accompanying drawings and detailed description.
Description of the drawings
Fig. 1 is the structural diagram of the present invention.
Fig. 2 is the structural schematic diagram of the single kidney bean frame of the present invention.
Figure label is, the box body (1) of groove profile, kidney bean frame (2), side inverted concave plate A (2.1), undercut before and after two pieces Shape plate B (2.2), expanded sheet metal (2.3), fine sand (3), filter paper (4), handle (5).
Specific implementation mode
In order to make those skilled in the art, methods of this invention will be better understood, with reference to specific embodiment to this hair It is bright to be described in further detail.
Fig. 1 is seen, shown in Fig. 2.The device for preventing feed mouldy in a kind of Frankliniella occidentalis feeding process, the present invention device packet The box body 1 that a top is set as open groove profile is included, handle 5 is provided on the lateral wall of box body 1;It is equipped in box body 1 Fine sand 3 is equipped with filter paper 4 on fine sand 3, and kidney bean frame 2 is arranged on filter paper 4;3 thickness of fine sand is set as 1cm- 3cm;The kidney bean frame 2 includes inverted concave plate B2.2 before and after two pieces of side inverted concave plate A2.1 and two pieces, four pieces of inverted concaves Plate fastens to form one piece of rectangular channel box, and stainless steel cloth plate 2.3 is equipped in the rectangular channel box, forms the four seasons of a rectangle Beans frame;The quantity of kidney bean frame 2 is set as N number of, and as N >=3, the box body bottom of groove profile is provided with 2, remaining kidney bean frame On folded pagoda pattern 2 kidney bean framves arranged at the bottom;The area of the filter paper 4 of the laying is greater than or equal to the four seasons The area that beans frame 2 projects downward vertically.Kidney bean can be placed on kidney bean frame using this kidney bean apparatus for placing and reach logical It can be provide one in insect cage that the effect of wind, and the utilization of sand table is placed conveniently in insect cage, which is moistening in sand table, Fixed humidity ensures thrips survival.
Be further that folded pagoda pattern of the present invention is, the side inverted concave plate A2.1 of the kidney bean frame and The side of front and back inverted concave plate B2.2 is both provided with the vertical interpolation slit being mutually matched and vertical cutting, and the vertical cutting is inserted into To fix kidney bean frame in vertical interpolation slit;The kidney bean frame bottom of last layer and next layer of kidney bean top of the trellis are also provided with phase Mutual matched horizontal interpolation slit and horizontal cutting, the vertical cutting are inserted into vertical interpolation slit to fix the kidney bean of combination Frame.
It is further that of the present invention to be equipped with stainless steel cloth plate 2.3 in the rectangular channel box, structure is, The inner wall middle and upper part of side inverted concave plate A2.1 and front and back inverted concave plate B2.2 are provided with the support horizontal stripe of a circle continuously or discontinuously, The stainless steel wire web plate is arranged on the support horizontal stripe;Or, four sides of stainless steel wire web plate are provided with several small hooks, When stainless steel wire web plate is filled in rectangular channel box, the small top edge for catching on four wall of rectangular channel box of linking up with is to support stainless steel cloth Plate.
It is further that filter paper 4 of the present invention is circular filter paper.
It is further that four pieces of notch plate materials of kidney bean frame of the present invention are wooden;It is sized to:Side undercut Shape plate A2.1, specification are long 15cm, high 8cm, deep 4cm, the wide 4cm of concave side;Front and back inverted concave plate B2.2, specification is long 8cm, high 8cm, deep 4cm, the wide 2cm of concave side, in kidney bean frame 2 have 2.3 mesh number of stainless steel wire web plate be 2-6 mesh, web plate position away from The distance of top top opening is 2cm.Kidney bean adds to solve using stainless steel is chronically at high humidity environment with wooden materials In the problem rotted, while without using irony frame because it gets rusty easily, both the above material is all unfavorable for extending making for this device With the service life, stainless steel frame, which has, makes simple, stable structure, will not corrosion the characteristics of;Kidney bean frame is solved using this specification Too small and excessive kidney bean can fall the problem of being difficult to place, and bottom is excessive in kidney bean frame height, place it is unstable and for If thrips nymph is difficult to be difficult to return on filter paper on Kidney bean and occur the loss of borer population.
Use the method for the device for preventing feed mouldy in a kind of above-mentioned Frankliniella occidentalis feeding process, this method packet of the present invention It includes and is set to the device in the insect cage of raising Frankliniella occidentalis, insect cage is placed in insectary or in growth cabinet; When the device is placed on insectary, which is, temperature is 26~28 DEG C, raising humidity is 60%~ 70%;When the device to be placed in growth cabinet, growth cabinet condition setting is that temperature is 26~28 DEG C, illumination Shading value L:D=16:8, raising humidity is 70%~80%.Be conducive to the preservation of Kidney bean under this humiture and more beneficial be The existence of Frankliniella occidentalis, humidity is excessively high to make the too fast drying of Kidney bean, the excessively high growth and development for being unfavorable for Frankliniella occidentalis of humidity.
It is further that this method of the present invention includes the chemical pretreatment of kidney bean, chemical pretreatment includes the following steps, It is fresh-keeping mould proof according to the component configuration of sodium benzoate 0.1-0.3g, potassium sorbate 5g, citric acid 15g, sodium citrate 15g, water 1L Agent, after kidney bean is peeled off throw impregnated in the fresh-keeping mould inhibitor of configuration after five minutes take out dry it is spare.
Be further that this method of the present invention includes the seed selection of kidney bean, that is, select resistance is stronger, after pesticide treatments It is not easy mouldy U.S.'s provider's kind or the kind of Nanjing ideal seedling Co., Ltd supply.
Be further that this method of the present invention includes the implantation methods of kidney bean, in the greenhouse will after presprouting of seeds, plant in In greenhouse in nylon gauze, insect infestations are prevented;During experiment, a water is poured within every 2~3 days, until being picked when kidney bean maturation It is spare;Any pesticide is not sprayed during experiment.
It is further that this method of the present invention includes following sequential steps, step 1:Fresh-keeping mould inhibitor is configured, in electronic scale On weigh sodium benzoate 0.2g, potassium sorbate 5g, citric acid 15g sodium citrate 15g, with purification water dissolution after be diluted to 1L, fill Enter spare in reagent bottle;
Step 2:The fine sand for cleaning drying is put in box body, fine sand thickness is 1-3cm, by the fresh-keeping anti-of above-mentioned configuration Mould dose is poured into fine sand, and agent liquid of being subject to does not agglomerate spilling on fine sand surface, after spread and filter paper and placed at least two four seasons Whole device is placed into Frankliniella occidentalis insect cage by beans frame;The fine sand of moistening can be that Frankliniella occidentalis supports worm to a certain extent Cage provides humidity, is covered with circular filter paper, a diameter of 15cm of filter paper, for specification relatively conventional in laboratory, this design thereon There is following benefit, on the one hand can prevent from growing on filter paper and fine sand containing above-mentioned fresh-keeping mould inhibitor in the fine sand of moistening mould Bacterium, another aspect filter paper be white, if having when thrips falls to bottom to observe in time, and filter paper block after can prevent Only thrips contacts with sand and loses polypide;
Step 3:Impregnate in above-mentioned fresh-keeping mould inhibitor to take out after five minutes after kidney bean is peeled off and dry, after be put into it is foster On a holder in worm cage, the quantity for placing kidney bean is suitably adjusted according to insect cage size, is at least covered entire Frame upper;It impregnates to allow fresh-keeping mould inhibitor to be effectively attached to kidney bean surface, raising is carried out after drying can prevent Kidney bean table Surface humidity is excessive to cause Frankliniella occidentalis dead;
Step 4:The kidney bean after fresh-keeping mould inhibitor impregnates is put after 5-8 days on another holder, is placed in first time Kidney bean observes the kidney bean on holder after 8-10 days, at this time under this environmental condition, the kidney bean on holder has been dried, and And it is not mouldy, will be dried and determine cleans out insect cage without thrips in kidney bean above;And so on four on each holder Season, beans were divided into 8-10 days between replacing, and the time difference on two holders is 5-6 days.
Specific embodiment is described in detail.
Concrete operations example:
With reference to specific embodiment, the present invention is furture elucidated, and present embodiment is being with technical solution of the present invention Under the premise of implemented, it should be understood that these modes are only illustrative of the invention and is not intended to limit the scope of the invention.
(1) Kidney bean apparatus for placing is:
Summary:Including box body, the kidney bean frame of kidney bean is placed, kidney bean frame is 4;Have in box body and cleans the thin of drying Sand, fine sand thickness are 2cm, the handle composition being laid on the circular filter paper and box body on fine sand;The above-mentioned entire dress In Frankliniella occidentalis insect cage, insect cage can be placed in insectary or in growth cabinet placement location.
(2) fresh-keeping mould inhibitor is:
Weighing group is divided into:Sodium benzoate 0.20g, potassium sorbate 5.00g, citric acid 15.00g, sodium citrate 15.00g add 1L is configured to after water dissolution.
(3) kidney bean is:
Kidney bean (Phaseolus vulgaris L.) selects the stronger kind insecticide of resistance for examination kidney bean seed Be not easy after processing it is mouldy, such as U.S.'s provider's kind, Nanjing ideal seedling Co., Ltd supply, in the greenhouse by presprouting of seeds Afterwards, plantation prevents insect infestations in nylon gauze in greenhouse.During experiment, pour a water within every 2~3 days, until kidney bean at It is picked when ripe to thrips to raise in cylinder and be tested.Any pesticide is not sprayed during experiment, kidney bean grows fine.
(3) selected insect source:
Frankliniella occidentalis and flower thrips worm sources for examination pick up from Yunnan Province Kunming Panlong District in May, 2017 and put on village Wild Hypericum Chinense (Hypericum monogynum L.) is taken, and is taken back laboratory progress Identification of Species and established population, is chosen The healthy adult of two kinds of thrips establishes population.To ensure that two kinds of thrips populations needed for this experiment are all from identical group, adopted Two kinds of thrips of collection separately carry out 5 with Kidney bean in Yunnan Prov Agriculture University's biological invasion research department growth cabinet and more than generation purify Raising.
(4) morphological feature of Frankliniella occidentalis:
Female adult pest:Body grows 1.4~1.7mm, and body colour is faint yellow, and to brown, variation is larger, and feeler 8 saves, and Section 1 is light yellow, Section 2 brown, the 3rd~5 section yellow, but end is slightly brown, the 6th~8 section brown.There is forked sense cone on 3rd, 4 sections.It is wide bigger In long, write before simple eye shorter, it is simple eye soft 1 pair, very long, it is located at the inside of front and back simple eye outer line, fresh 6 pairs after compound eye, 4th pair of longest in riotous profusion from inside to outside, it is about isometric with simple eye hair on the neck.Pronotary has 4 pairs of long collection, is located at leading edge, left and right anterior angle Each 1 pair, left and right relief angle 2 is right, rear center have 5 pairs it is in riotous profusion, wherein from the outside 2nd pair of longest in riotous profusion in center;Middle metanotum healing, after There is irregular netted line in thoracic dorsal plate center, has a pair of of mitriform perceptron (be in bright spot shape) close to rear, in metanotum leading edge There are 2 pairs of long hairs on the neck in centre.Fore wing is faint yellow, upper arteries and veins ticket 18~21,13~16 in riotous profusion of lower arteries and veins, and arrangement is uniformly complete.Abdomen the 5th~8 Section backboard both sides have micro-bend comb, the 8th backboard rear to have 12 1 skilful root of pectination hair.Section 9 backboard has 2 pairs of mitriform perceptrons.3rd~ 7 web rears have 3 pairs in riotous profusion, without attached hair on the neck.
Male imago:Body grows 1~1.15mm, body yellow, and the 3rd~7 apotome of abdomen has a small cross oval gland room, and the 8th Backboard rear is saved without pectination hair.
(5) method of contrast:
Experiment one, respectively use are traditional not to be added kidney bean beans apparatus for placing and fresh-keeping mould inhibitor (conventional husbandry method), makes It is not added with fresh-keeping mould inhibitor (Kidney bean holds method), using Kidney bean apparatus for placing and fresh-keeping mould inhibitor (fresh-keeping Sheng with Kidney bean apparatus for placing Put method) three kinds of experimental methods (raise Frankliniella occidentalis in long by 40 × wide by 30 × high 30), 15 are placed every time in insect cage in insect cage Root vegetables beans record mouldy kidney bean quantity respectively in 3 days, 6 days, 9 days observation mouldy situations of kidney bean.
Experiment two is put into purified water in the batch cultur ware (d=25mm) and fresh-keeping mould inhibitor impregnated about 2cm's long Kidney bean, in the nymph to culture dish with the careful picking single head thrips of fine, soft fur pen, to prevent thrips from escaping from culture dish, culture Ware lid covers and winds upper preservative film in interface, per 1 processed Kidney bean of clear water is replaced for 24 hours, each control and processing Nymph afterwards observes 100 respectively, and the developmental state and survival condition of all thrips are observed every 12h.
Experiment three differentiates two kinds of thrips under anatomical lens, then takes respectively 50 to (female:Male=1:1) adult, by single pair Ji Horse accesses in above-mentioned culture dish (d=25mm), and per 1 Kidney bean is replaced for 24 hours, the survival of primary female male imago is observed every 12h Situation, raising Kidney bean is respectively purified water and the processed Kidney bean of fresh-keeping mould inhibitor, and fresh-keeping mould inhibitor is measured to male and female with this The influence of adult.
It is the data analysis to traping result below
(6) three kinds of distinct methods raise the mouldy quantity of Frankliniella occidentalis Kidney bean
1 three kinds of distinct methods raising mouldy quantity of Frankliniella occidentalis Kidney bean of table compare
As can be seen from Table 1, it in three kinds are put the raising of Frankliniella occidentalis, is observed and recorded respectively at 3d, 6d, 9d When, just have in 3d in conventional husbandry method 20.00% Kidney bean it is mouldy, and as number of days increases, because mould is big in environment Amount breeding so that infect quickenings, the mouldy rate of Kidney bean is 53.33% when 6d, the raising of unsuitable Frankliniella occidentalis at this time, If continue to raise has had 93.33% Kidney bean that mouldy situation has occurred when to 9d, all Kidney beans have almost been infected. And using in the fresh-keeping Frankliniella occidentalis for holding method raising, mouldy Kidney bean is found no in 3-6d, is supported in part in experiment Observe that small part mould is also not up to serious situation in worm cage, there is no the existence of excessive interference to Frankliniella occidentalis.
(7) fresh-keeping mould inhibitor uses the influence to Frankliniella occidentalis nymph
2 fresh-keeping mould inhibitor of table uses the comparison to Frankliniella occidentalis nymph survival rate
It can be obtained by table 2 two, Frankliniella occidentalis is raised not in the Kidney bean impregnated respectively using fresh-keeping mould inhibitor and purified water With age nymph when, though each age there is different degrees of difference to be not up to significant difference, it is entire for the ripe worm phase Cumulative survival rate be:The thrips of the Kidney bean raising handled using fresh-keeping mould inhibitor is slightly less than the dish that purified water impregnated raising Beans, respectively 62.19% and 67.06%, difference 4.87%, but the not up to level of signifiance.Fresh-keeping mould inhibitor is to Frankliniella occidentalis It is influenced there may be certain, but influence is not notable, to the raising safety of Frankliniella occidentalis, will not be caused to Frankliniella occidentalis larger It influences.
(8) fresh-keeping mould inhibitor uses the influence to Frankliniella occidentalis different larval instar development duration
3 fresh-keeping mould inhibitor of table uses the comparison to Frankliniella occidentalis different larval instar development duration
It can be obtained by table 3, be raised in the Frankliniella occidentalis of the Kidney bean raising handled using fresh-keeping mould inhibitor and untreated Kidney bean It supports and compares, except 2 age nymph, two kinds of Kidney bean raisings are with (F in addition to significant difference1,75=4.613, P<0.05), remaining each age Without significant difference (P<0.05), interim in entire prematurity worm, the Frankliniella occidentalis hair of the Kidney bean raising of fresh-keeping mould inhibitor processing Educating the phase of going through is slightly less than untreated, but difference is not notable, is 0.33d less than the time;Have in being to test to female male imago:Place The female adult pest service life after reason is slightly less than untreated, does not reach the level of signifiance, time difference 0.29d, and treated male imago longevity Order it is a little higher than untreated, do not reach the level of signifiance, higher than the time be 0.60d.But thrips Survival is not significantly impacted.
By three above the experiment results show that when being raised to Frankliniella occidentalis using three kinds of methods, there is fresh-keeping hold Method holds method better than Kidney bean and is better than conventional husbandry method, and the utilization of kidney bean frame and the utilization of fresh-keeping mould inhibitor can be to a certain degree It is upper to ensure that mouldy problem does not occur for the Kidney bean of raising Frankliniella occidentalis, it may be influenced by component environment in particular surroundings, still Setting ratio when can guarantee indoor raising under conventinal breeding.Furthermore it is fresh-keeping to the survival rate of Frankliniella occidentalis each age and It can be obtained in the development duration experiment of each age, the utilization of fresh-keeping mould inhibitor may be to a certain extent to the survival of Frankliniella occidentalis And growth has an impact, but compared with the efficiency that the expansion of thrips is numerous and raises, excessive resistance can't be generated to Frankliniella occidentalis Hinder, can guarantee that the when of raising Frankliniella occidentalis indoors quickly improves feeding efficiency, expand thrips population, and with easy to operate, The features such as being easy to grasp can quickly carry out population raising and play certain positive effect in the study on prevention of Frankliniella occidentalis.
The method and result that prevent the feeds such as kidney bean mouldy in Frankliniella occidentalis feeding process have been carried out specifically above It is bright, as long as but slightly being adjusted using the different collocation and proportioning of the method and structure of the present invention and fresh-keeping mould inhibitor, such as four Season beans posture sample, fresh-keeping mould inhibitor proportioning etc., can be to shaped like such thrips such as raising Frankliniella occidentalis, flower thrips, yellow chest thrips Class pest and the common natural enemy Similis of thrips are raised.
Above-described is only specific embodiments of the present invention, and the common sense such as well known concrete structure and characteristic are herein in scheme It does not describe excessively.It should be pointed out that for those skilled in the art, without departing from the structure of the invention, also Several modifications and improvements can be made, these should also be considered as protection scope of the present invention, these all do not interfere with of the invention real The effect and patent practicability applied.The scope of protection required by this application should be based on the content of the claims, specification In the records such as specific implementation mode can be used for explaining the content of claim.

Claims (10)

1. the device for preventing feed mouldy in a kind of Frankliniella occidentalis feeding process, which is characterized in that the device includes a top It is set as the box body (1) of open groove profile, handle (5) is provided on the lateral wall of box body (1);It is equipped in box body (1) thin Husky (3), are equipped with filter paper (4) on fine sand (3), and kidney bean frame (2) is arranged on filter paper (4);Described fine sand (3) thickness is set It is set to 1cm-3cm;The kidney bean frame (2) includes inverted concave plate B before and after two pieces of side inverted concave plate A (2.1) and two pieces (2.2), four blocks of inverted concave plates fasten to form one piece of rectangular channel box, and stainless steel cloth plate (2.3) is equipped in the rectangular channel box, Form the kidney bean frame of a rectangle;The quantity of kidney bean frame (2) is set as N number of, and as N >=3, kidney bean frame bottom is provided with 2, remaining kidney bean frame is on folded pagoda pattern 2 kidney bean framves arranged at the bottom;The filter paper (4) of the laying Area be greater than or equal to the area that projects downward vertically of kidney bean frame (2).
2. the device for preventing feed mouldy in a kind of Frankliniella occidentalis feeding process according to claim 1, which is characterized in that The folded pagoda pattern is the side of the side inverted concave plate A (2.1) of the kidney bean frame and front and back inverted concave plate B (2.2) While being both provided with the vertical interpolation slit being mutually matched and vertical cutting, the vertical cutting is inserted into vertical interpolation slit to fix the four seasons Beans frame;The kidney bean frame bottom of last layer and next layer of kidney bean top of the trellis are also provided with the horizontal interpolation slit and water being mutually matched Plug flat item, the vertical cutting are inserted into vertical interpolation slit to fix the kidney bean frame of combination.
3. the device for preventing feed mouldy in a kind of Frankliniella occidentalis feeding process according to claim 1, which is characterized in that Described is equipped with stainless steel cloth plate (2.3) in the rectangular channel box, and structure is, side inverted concave plate A (2.1) and before The inner wall middle and upper part of inverted concave plate B (2.2) is provided with the support horizontal stripe of a circle continuously or discontinuously, the stainless steel wire web plate afterwards It is arranged on the support horizontal stripe;Or, several small hooks are provided on four sides of stainless steel wire web plate, when stainless steel wire web plate is filled in When in rectangular channel box, small hook catches on the top edge of four wall of rectangular channel box to support stainless steel wire web plate.
4. the device for preventing feed mouldy in a kind of Frankliniella occidentalis feeding process according to claim 1, which is characterized in that The filter paper (4) is circular filter paper.
5. the device for preventing feed mouldy in a kind of Frankliniella occidentalis feeding process according to claim 1, which is characterized in that Described four pieces of notch plate materials of kidney bean frame are wooden;It is sized to:Side inverted concave plate A (2.1), specification are length 15cm, high 8cm, deep 4cm, the wide 4cm of concave side;Front and back inverted concave plate B (2.2), specification are long 8cm, high 8cm, deep 4cm, and concave side is wide 2cm, in kidney bean frame (2) have stainless steel wire web plate (2.3) mesh number be 2-6 mesh, web plate position far from top top opening away from From for 2cm.
6. using the side for the device for preventing feed mouldy in a kind of any Frankliniella occidentalis feeding process of claim 1 to 5 Method, which is characterized in that this method includes being set to the device in the insect cage of raising Frankliniella occidentalis, insect cage is placed on foster In zooecium or in growth cabinet;When the device is placed on insectary, which is that temperature is 26~28 DEG C, raising humidity is 60%~70%;When the device to be placed in growth cabinet, growth cabinet condition setting is temperature Degree is 26~28 DEG C, the shading value L of illumination:D=16:8, raising humidity is 70%~80%.
7. the application method for the device for preventing feed mouldy in a kind of Frankliniella occidentalis feeding process according to claim 6, It is characterized in that, this method includes the chemical pretreatment of kidney bean, chemical pretreatment includes the following steps, according to sodium benzoate The fresh-keeping mould inhibitor of component configuration of 0.1-0.3g, potassium sorbate 5g, citric acid 15g, sodium citrate 15g, water 1L, kidney bean is shelled After opening throw impregnated in the fresh-keeping mould inhibitor of configuration after five minutes take out dry it is spare.
8. the application method for the device for preventing feed mouldy in a kind of Frankliniella occidentalis feeding process according to claim 6, It is characterized in that, this method includes the seed selection of kidney bean, that is, select that resistance is stronger, is not easy mouldy U.S. after pesticide treatments State's provider's kind or the kind of Nanjing ideal seedling Co., Ltd supply.
9. the application method for the device for preventing feed mouldy in a kind of Frankliniella occidentalis feeding process according to claim 6, It is characterized in that, this method includes the implantation methods of kidney bean, in the greenhouse by after presprouting of seeds, plant in nylon yarn in greenhouse In net, insect infestations are prevented;During experiment, a water is poured within every 2~3 days, until being picked when kidney bean maturation spare;During experiment Any pesticide is not sprayed.
10. the application method for the device for preventing feed mouldy in a kind of Frankliniella occidentalis feeding process according to claim 6, It is characterized in that, this method includes following sequential steps, step 1:Fresh-keeping mould inhibitor is configured, benzoic acid is weighed on electronic scale Sodium 0.2g, potassium sorbate 5g, citric acid 15g sodium citrate 15g are fitted into standby in reagent bottle with 1L is diluted to after purification water dissolution With;
Step 2:The fine sand (3) for cleaning drying is put in box body, fine sand (3) thickness is 1-3cm, by the fresh-keeping of above-mentioned configuration Mould inhibitor pours into fine sand (3), and agent liquid of being subject to does not agglomerate spilling on fine sand (3) surface, after spread and filter paper and place at least Two kidney bean framves, whole device is placed into Frankliniella occidentalis insect cage;
Step 3:Impregnate in above-mentioned fresh-keeping mould inhibitor to take out after five minutes after kidney bean is peeled off and dry, after be put into insect cage In a holder on, the quantity for placing kidney bean is suitably adjusted according to insect cage size, at least covers entire holder Top;
Step 4:The kidney bean after fresh-keeping mould inhibitor impregnates is put after 5-8 days on another holder, in the four seasons that first time places Beans observe the kidney bean on holder after 8-10 days, at this time under this environmental condition, the kidney bean on holder has been dried, and not Mouldy, will be dried and determine cleans out insect cage without thrips in kidney bean above;The and so on kidney bean on each holder It is divided between replacement 8-10 days, the time difference on two holders is 5-6 days.
CN201810388977.3A 2018-04-27 2018-04-27 Device for preventing feed from mildew in frankliniella occidentalis feeding process and using method Active CN108684614B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810388977.3A CN108684614B (en) 2018-04-27 2018-04-27 Device for preventing feed from mildew in frankliniella occidentalis feeding process and using method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810388977.3A CN108684614B (en) 2018-04-27 2018-04-27 Device for preventing feed from mildew in frankliniella occidentalis feeding process and using method

Publications (2)

Publication Number Publication Date
CN108684614A true CN108684614A (en) 2018-10-23
CN108684614B CN108684614B (en) 2023-12-12

Family

ID=63845812

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201810388977.3A Active CN108684614B (en) 2018-04-27 2018-04-27 Device for preventing feed from mildew in frankliniella occidentalis feeding process and using method

Country Status (1)

Country Link
CN (1) CN108684614B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111543395A (en) * 2020-05-28 2020-08-18 云南农业大学 Propagation method for population quantity of frankliniella occidentalis
CN112931417A (en) * 2021-02-26 2021-06-11 中国农业科学院植物保护研究所 Method for group breeding of frankliniella occidentalis and application thereof

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102523974A (en) * 2012-03-06 2012-07-04 南京农业大学 Crop and insect co-cultivation box
CN102640729A (en) * 2012-04-06 2012-08-22 山东省农业科学院植物保护研究所 Method for breeding frankliniella occidentalis
CN203814416U (en) * 2014-05-05 2014-09-10 辽宁华电环保科技有限公司 Three-dimensional multi-layer push-and-pull-type earthworm breeding bed
CN203897070U (en) * 2014-05-30 2014-10-29 贵州大学 Indoor prodenia litura larva raising device
CN104206362A (en) * 2014-09-19 2014-12-17 贵州大学 Phytotron for feeding prodenia litura and indoor artificial breeding method
CN204599046U (en) * 2015-02-10 2015-09-02 山东省农业科学院植物保护研究所 A kind of fragrant-flowered garlic maggot raising device
US20160066552A1 (en) * 2013-04-19 2016-03-10 Protix Biosystems B.V. Method and System for Breeding Insects, Using a Plurality of Individual Crates
CN205813293U (en) * 2016-06-14 2016-12-21 西南大学柑桔研究所 Predatory Mites breeding apparatus
CN106578839A (en) * 2016-11-25 2017-04-26 广州市义和化工有限公司 Feed mildew proofing agent and preparation method thereof
CN106879557A (en) * 2017-02-17 2017-06-23 中国农业科学院棉花研究所 A kind of method for raising black striped plant bug
CN208402950U (en) * 2018-04-27 2019-01-22 云南农业大学 A kind of device for preventing feed mouldy in Frankliniella occidentalis breeding process

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102523974A (en) * 2012-03-06 2012-07-04 南京农业大学 Crop and insect co-cultivation box
CN102640729A (en) * 2012-04-06 2012-08-22 山东省农业科学院植物保护研究所 Method for breeding frankliniella occidentalis
US20160066552A1 (en) * 2013-04-19 2016-03-10 Protix Biosystems B.V. Method and System for Breeding Insects, Using a Plurality of Individual Crates
CN203814416U (en) * 2014-05-05 2014-09-10 辽宁华电环保科技有限公司 Three-dimensional multi-layer push-and-pull-type earthworm breeding bed
CN203897070U (en) * 2014-05-30 2014-10-29 贵州大学 Indoor prodenia litura larva raising device
CN104206362A (en) * 2014-09-19 2014-12-17 贵州大学 Phytotron for feeding prodenia litura and indoor artificial breeding method
CN204599046U (en) * 2015-02-10 2015-09-02 山东省农业科学院植物保护研究所 A kind of fragrant-flowered garlic maggot raising device
CN205813293U (en) * 2016-06-14 2016-12-21 西南大学柑桔研究所 Predatory Mites breeding apparatus
CN106578839A (en) * 2016-11-25 2017-04-26 广州市义和化工有限公司 Feed mildew proofing agent and preparation method thereof
CN106879557A (en) * 2017-02-17 2017-06-23 中国农业科学院棉花研究所 A kind of method for raising black striped plant bug
CN208402950U (en) * 2018-04-27 2019-01-22 云南农业大学 A kind of device for preventing feed mouldy in Frankliniella occidentalis breeding process

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111543395A (en) * 2020-05-28 2020-08-18 云南农业大学 Propagation method for population quantity of frankliniella occidentalis
CN112931417A (en) * 2021-02-26 2021-06-11 中国农业科学院植物保护研究所 Method for group breeding of frankliniella occidentalis and application thereof

Also Published As

Publication number Publication date
CN108684614B (en) 2023-12-12

Similar Documents

Publication Publication Date Title
Reinhardt et al. Advances in pineapple plant propagation
CN102318547B (en) Overhead cultivation method of Dendrobium huoshanense test-tube seedlings
CN105519367B (en) Wild cotton seedling stage low temperature resistant identification method
Luna et al. Seed germination and sowing options
CN103155906A (en) Aphidoletes aphidimyza mass reproduction technology and application technology
CN105104076B (en) A kind of method for accelerating the growth of batch production tea shoot
CN104365557B (en) A kind of Cryptolaemus montrouzieri artificial breeding, conservation and propagation production method
CN105144911B (en) A kind of straw or like vegetable cultural method
CN113287513A (en) Eggplant haploid plant sexual propagation doubling method based on improvement of pollen activity
CN109121783A (en) The engrafting method of twig will not be sprouted using oil-tea sprout radicle graft stock part
CN208402950U (en) A kind of device for preventing feed mouldy in Frankliniella occidentalis breeding process
CN108684614A (en) The device and application method for preventing feed mouldy in a kind of Frankliniella occidentalis feeding process
CN105684596B (en) The method that half capsule lettuce tongue seed is promoted to sprout
CN107197746A (en) A kind of mating system of China fir field excellent resources
CN106613274A (en) Seedling raising method achieved by means of aconitum kusnezoffii Reichb. seeds
CN106106375A (en) A kind of method of water planting Caulis et Folium Brassicae capitatae indoor mass rearing diamondback moth
CN107787698A (en) A kind of production method for the seed potato for avoiding soil-borne disease from infecting
CN105393986B (en) A method of the parasitic wasp of release Chilo spp larvae
WO2015003546A1 (en) Culture method for natural species rejuvenation of artificially bred cordyceps host insect
CN106135166B (en) Propagation method suitable for common green lacewing and selected device
Kains Propagation Of Plants-A Complete Guide For Professional And Amateur Growers Of Plants By Seeds, Layers, Grafting And Budding, With Chapters On Nursery And Greenhouse Management
Splawski et al. Mulch effects on floral resources and fruit production of squash, and on pollination and nesting by squash bees
CN212589712U (en) Biological observation device for breeding life of overwintering imagoes of castanea mollissima
CN102771446A (en) Propagation method of Diglyphus isaea by use of Chromatomyia horticola
CN104396890B (en) Midge intensive farm and chemicals biologically active measurement apparatus and method

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant