CN108680532A - Biosensor and preparation method thereof based on optical fiber mach-Zeng Deer interferometers - Google Patents
Biosensor and preparation method thereof based on optical fiber mach-Zeng Deer interferometers Download PDFInfo
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- CN108680532A CN108680532A CN201810783322.6A CN201810783322A CN108680532A CN 108680532 A CN108680532 A CN 108680532A CN 201810783322 A CN201810783322 A CN 201810783322A CN 108680532 A CN108680532 A CN 108680532A
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- optical fiber
- glass tube
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
- G01N21/45—Refractivity; Phase-affecting properties, e.g. optical path length using interferometric methods; using Schlieren methods
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
Abstract
The present invention provides a kind of biosensors and preparation method thereof based on optical fiber mach Zeng Deer interferometers, concentration for detecting biological tissue's solution, solve in manufacturing process that producer is more dangerous, and the problem that biosensor reliability, sensitivity are low；It includes：Micro flow chip offers microchannel in micro flow chip, and micro flow chip offers inlet, and inlet is connected to microchannel；The light guide assemblies being arranged in microchannel, light guide assemblies offer MZ chambers, and MZ chambers are connected to microchannel；Light source in micro flow chip side is set, part is provided；And the spectrometer in micro flow chip side is set；The setting of light guide assemblies is avoided using dangerous corrosive liquid during making biosensor, to make producer be ensured safely.
Description
Technical field
The invention belongs to field of biosensors more particularly to a kind of biologies based on optical fiber mach-Zeng Deer interferometers
Sensor and preparation method thereof.
Background technology
Mach-Zehnder (Mach-Zehnder, MZ) interferometer is a kind of interferometer, can be used for observing from independent light source
The beam splitting of transmitting changes at after twice collimated light beam by relative phase shift caused by different paths and medium.Mach-was once
Dare interferometer in various fields there is extensive use, one of which application to be namely based on the biosensor of MZ interferometers.So
And existing based on the biosensor of optical fiber MZ interferometer, there are many defects.During making biosensor, need
Optical fiber is processed, some processing methods are needed using unwanted corrosion liquid (such as：The chemical agents such as hydrofluoric acid) it is right
Inside of optical fibre is corroded to form MZ chambers, and these corrosive liquids may damage processing staff, thereby using this
Processing method is processed in the presence of greatly dangerous optical fiber.
In general, the existing biosensor based on optical fiber MZ interferometer in the production process, the peace of producer
It cannot ensure entirely.
Invention content
Technical problem to be solved by the present invention lies in provide a kind of biology biography based on optical fiber mach-Zeng Deer interferometers
Sensor and preparation method thereof, it is intended to solve it is existing based on the biosensor of optical fiber MZ interferometer during making, system
The problem of making personnel's more danger and making the safety of producer that cannot ensure.
In order to solve the above technical problems, the invention is realized in this way, it is a kind of based on optical fiber mach-Zeng Deer interferometers
Biosensor, which is characterized in that including：Micro flow chip offers microchannel, the micro flow chip in the micro flow chip
Inlet is offered, the inlet is connected with the microchannel；Light guide assemblies in the microchannel, institute are set
It states and offers MZ chambers in light guide assemblies, the MZ chambers are connected with the microchannel；It is arranged in the micro flow chip side
Light source provides part, and the light source provides part and is connected with one end of the light guide assemblies；And it is arranged in the micro flow chip one
The spectrometer of side, one end that the spectrometer provides part with the light guide assemblies far from the light source are connected.
By using above-mentioned technical proposal, when using the biosensor based on optical fiber mach-Zeng Deer interferometers, first
By biological tissue's solution out of inlet injection microchannel, and biological tissue's solution is made to enter the MZ chambers in light guide assemblies,
After the optical signal that light source offer part provides enters light guide assemblies, and when optical signal enters MZ chambers, optical signal enters biology simultaneously
The reference arm that the interfere arm and light guide assemblies itself that tissue solution is formed are formed, in optical signal after MZ chambers, in light guide group
Aggregation in part, since the optical index between interfere arm and reference arm has differences, to make optical signal generate refringence, from
And optical path difference is generated, interference is formed, last optical signal is incident upon from light-emitting window on spectrometer, to form interference spectrum, at this
In invention, using light guide assemblies instead of optical fiber in the prior art, since light guide assemblies offer MZ chambers, so there is no need to use hydrogen fluorine
The hazardous chemicals medicament such as acid corrodes light guide assemblies, so during making biosensor, makes producer's
Safety is ensured, and without carrying out drawing cone processing to light guide assemblies, can not only reduce cost of manufacture, and can
So that each position diameter of light guide assemblies is consistent, to reduce light loss, improves the sensitivity of biosensor, make biology
Sensor is relatively reliable.
Further, the light guide assemblies include：First optical fiber；Light guide in first optical fiber one end, institute are set
It states and offers cavity inside light guide, the cavity is MZ chambers；And it is arranged in the light guide far from first optical fiber one
Second optical fiber at end, first optical fiber and second optical fiber are symmetrical arranged along the light guide.
Further, the light guide includes：Glass tube, the opposite end of the glass tube respectively with first optical fiber
And the second optical fiber phase welding, the glass tube side offer communication port, the communication port both ends are respectively to described first
Optical fiber and second extension of optical fiber, and extend to the opposite both ends of the glass tube, the MZ chambers by the communication port with
The external world is connected；And it is filled in the filling member of MZ intracavitary, the diameter of the filling member is less than the diameter of the MZ chambers, described
First optical fiber and second fused fiber splice are on the inner wall that one end of the glass tube is respectively positioned on the cavity, and part and institute
Filling member is stated to be in contact.
Further, the filling member and the closure member are uv-curable glue, the refractive index of the uv-curable glue
It is 1.36.
It is reduced after the uv-curable glue filling glass pipe for being 1.36 with refractive index by using above-mentioned technical proposal
The refractive index of biosensor provided by the invention so that biosensor sensitivity provided by the invention is 40000nm/RIU,
And common biosensor sensitivity is 10000nm/RIU in the market, to improve the sensitivity of biosensor.
Further, the glass length of tube is 100 μm, and the glass tube external diameter and internal diameter are respectively 125 μm and 75 μm.
Further, first optical fiber and second optical fiber are single mode optical fiber fibre core.
Further, the light source provides part for providing wideband light source.
Further, plastic flexible pipe is provided at the inlet that the micro flow chip opens up.
Further, the micro flow chip offers liquid outlet, and the liquid outlet is connected with the microchannel, described
Micro flow chip side is provided with waste collecting device, and the waste collecting device is connected with the liquid outlet.
A kind of production method of the biosensor based on optical fiber mach-Zeng Deer interferometers, which is characterized in that the side
Method includes：By one end phase welding of the first optical fiber and glass tube, and accurate cutting processing is done to glass tube, glass tube is made to retain
100 μm of length, then in the second optical fiber of other end welding of glass tube；One side throwing of glass tube is removed, is thrown except thickness is 40 μ
M, to open up communication port on glass tube, so that the cavity of glass tube is connected with the external world；It is filled in glass tube purple
Outer solidification glue, and make ultra-violet curing adhesive curing, and the cavity for making the diameter of the uv-curable glue after solidification be less than in glass tube is straight
Diameter；Uv-curable glue extra in glass tube is removed, so that the uv-curable glue of glass tube reaches best interference light intensity pair
Degree of ratio；Microchannel is made on micro flow chip, and opens up inlet and liquid outlet；By glass tube, the first optical fiber and the second light
Fibre is placed in microchannel, and uv-curable glue is used in combination respectively to close the light inlet at microchannel both ends and light-emitting window；By first
The one end of optical fiber far from glass tube provides part with light source and is connected, and the one end of the second optical fiber far from glass tube is connected with spectrometer
It connects.
Compared with prior art, the present invention advantageous effect is：By using glass tube both ends welding optic fibre, then from side
The method that face rubbing down is begun to speak replaces the cavity in the biosensor of MZ interferometers in the prior art, and the cavity in glass tube is made to make
For MZ chambers, optical fiber is corroded without hazardous chemicals medicaments such as use hydrofluoric acid, so making biosensor
In the process, the safety of producer is made to be ensured；And the filling member for being 1.36 by using refractive index is to glass tube
It is filled so that biosensor sensitivity provided by the invention is 40000nm/RIU, is passed for most of biologies in the market
4 times of sensor sensitivity, to improve the sensitivity of biosensor in the prior art.
Description of the drawings
Fig. 1 is the structural schematic diagram of biosensor of the embodiment of the present invention based on optical fiber mach-Zeng Deer interferometers, and
Section view processing is done to micro flow chip；
Fig. 2 is the micro flow chip and light of biosensor of the embodiment of the present invention based on optical fiber mach-Zeng Deer interferometers
The sectional view of guide assembly；
Fig. 3 is the analog simulation wave crest of biosensor of the embodiment of the present invention based on optical fiber mach-Zeng Deer interferometers
With the drift figure of variations in refractive index.
In the accompanying drawings, each reference numeral indicates：1, microchannel；101, syringe；102, light source provides part；103, feed liquor
Mouthful；104, liquid outlet；105, waste collecting device；106, spectrometer；107, micro flow chip；11, light inlet；12, light-emitting window；
13, light guide assemblies；131, glass tube；132, communication port；14, closure member；15, plastic flexible pipe；201, the first optical fiber；202、MZ
Chamber；203, filling member；204, the second optical fiber.
Specific implementation mode
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, and
It is not used in the restriction present invention.
A kind of biosensor based on optical fiber mach-Zeng Deer interferometers, as shown in Figures 1 and 2, including：Miniflow core
Piece 107, light guide assemblies 13, closure member 14, light source provide part 102 and spectrometer 106；It is logical that miniflow is offered in micro flow chip 107
Road 1, microchannel 1 are connected by light inlet 11 and light-emitting window 12 with the external world, and micro flow chip 107 offers inlet 103
And liquid outlet 104；Light guide assemblies 13 are arranged in microchannel 1, and MZ chambers 202 are offered in light guide assemblies 13, and MZ chambers
202 are connected with microchannel 1, and biological tissue's solution is entered by inlet 103 in microchannel 1 and MZ chambers 202, and raw
After object tissue solution enters in MZ chambers 202, as the interfere arm of biosensor, part light guide assemblies 13 are used as biosensor
Reference arm；Closure member 14 is arranged on micro flow chip 107, for after being placed in light guide assemblies 13 in microchannel 1, will go out
Optical port 12 and light inlet 11 are closed, to prevent biological tissue's solution from flowing out micro flow chip 107 from light-emitting window 12 and light inlet 11,
To reduce the probability that biological tissue's solution adversely affects light guide assemblies 13, while biological tissue's solution can be prevented
Waste, and reduce the probability that biological tissue's solution corrodes micro flow chip 107；Light source provides part 102 and is arranged in micro flow chip
107 open up the side of light inlet 11, for providing light source, to improve the spectral width of light source, to improve the survey of biosensor
Sensitivity is measured, in the present embodiment, light source provides part 102 for providing wideband light source；Spectrometer 106 is arranged in micro flow chip
107 open up the side of light-emitting window 12, when light source, which provides the broadband light that part 102 provides, passes through interfere arm and reference arm simultaneously, by
Optical index between interfere arm and reference arm has differences, to make broadband light generate refringence, to generate light path
Difference forms interference, the aggregation in light guide assemblies 13 of last broadband light, and is incident upon on spectrometer 106 from light-emitting window 12, to shape
At interference spectrum.
In the present embodiment, microchannel 1 is beeline channel, and light inlet 11 and light-emitting window 12 are symmetrical, set in this way
It sets, the distance and time that broadband light is needed across micro flow chip 107 can be reduced, to reduce light loss, improve bio-sensing
The detectivity of device.
Wherein, light guide assemblies 13 include：First optical fiber 201, light guide and the second optical fiber 204, the first optical fiber 201 and second
Optical fiber 204 is separately positioned on the opposite both ends of light guide, and cavity is offered inside light guide, and the cavity is as MZ chambers 202, filling
Part 203 is filled in cavity.
In the present embodiment, the first optical fiber 201 and the second optical fiber 204 are single mode optical fiber fibre core, since single-mode optics are long and slender
Core has the advantages that low-loss and minimum dispersion when transmitting optical signal, so as to improve the detection accuracy of biosensor.
Light guide includes：Glass tube 131 and filling member 203；First optical fiber 201 and the second optical fiber 204 are fused to glass tube
131 opposite both ends, the chamber in glass tube 131 offer communication port 132 as MZ chambers 202 in the side of glass tube 131,
Communication port 132 extends to the first optical fiber 201 and the second optical fiber 204 respectively, and extends to the opposite both ends of light guide, MZ chambers
202 are connected by communication port 132 with the external world；Filling member 203 is filled in MZ chambers 202, as the reference arm of biosensor,
And the diameter of filling member 203 is less than the diameter of cavity, in order to which biological tissue's solution flows into MZ chambers and forms interfere arm.
In the present embodiment, the first optical fiber 201 and the second optical fiber 204 are fused to one end of glass tube 131 and are located at cavity
On inner wall, and part is in contact with filling member 203, and the first optical fiber 201 is located at 11 side of light inlet of microchannel 1, and second
Optical fiber 204 is located at 12 side of light-emitting window of microchannel 1, in this way, in broadband light after the first optical fiber 201, convenient for width
Optical path difference is generated by the medium of different refractivity with light, to form interference, after broadband light passes through glass tube 131,
Converge at second optical fiber 204, the broadband light for having generated optical path difference is incident upon by the second optical fiber 204 on spectrometer 106, to shape
At interference spectrum.
As shown in Figures 2 and 3, in the present embodiment, filling member 203 and closure member 14 are uv-curable glue, ultraviolet solid
The refractive index for changing glue is 1.36, when using uv-curable glue as filling member 203, the refractive index of reference arm is made to be more nearly water
The refractive index of solution, to reduce the refractive index of biosensor provided by the invention so that biology provided by the invention passes
Sensor sensitivity is 40000nm/RIU, and common biosensor sensitivity is 10000nm/RIU in the market, to improve
The sensitivity of biosensor.When using uv-curable glue as closure member 14, due to uv-curable glue have quick solidifying,
Energy consumption less, solvent-free pollution the advantages that, so as to reduce the time of glue curing, reduce the waste of the energy, reduce to biology
Tissue solution adversely affects.
In the present embodiment, the length of glass tube 131 is 100 μm, and the internal diameter and outer diameter of glass tube 131 are respectively 125 μm
And 75 μm, the communication port 132 that glass tube 131 opens up is to throw except the thickness of 40 μm of 131 side of glass tube is formed.
Plastic flexible pipe 15 is provided at the inlet 103 of micro flow chip 107, the use of plastic flexible pipe 15 can be more
Convenient for importing biological tissue's solution in microchannel 1, in the present embodiment, using syringe 101 at a predetermined velocity to plastics
Hose 15 injects biological tissue's solution, and biological tissue's solution is imported microchannel 1.
It is provided with waste collecting device 105 at the liquid outlet 104 of micro flow chip 107, a biological tissue is molten obtaining
After the interference spectrum of liquid, biological tissue's solution in micro flow chip 107 is imported in waste collecting device 105, is used in combination ethyl alcohol clear
Microchannel 1 and light guide assemblies 13 are washed, are then imported ethyl alcohol in waste collecting device 105, waste collecting device 105 is set
It sets, can temporarily store waste liquid caused by many experiments, be required for launching waste liquid to prevent experimenter from testing every time
To waste liquid release position, the labor intensity of experimenter is reduced, and reduces the time that experimenter launches waste liquid, to improve
The efficiency of continuous several times experiment.
The present invention also provides a kind of production method of the biosensor based on optical fiber mach-Zeng Deer interferometers, on
The method of stating includes：By one end phase welding of the first optical fiber 201 and glass tube 131, and accurate cutting processing is done to glass tube 131,
Glass tube 131 is set to retain 100 μm of length, then in the second optical fiber of other end welding 204 of glass tube 131；By glass tube 131
A side throwing remove, throw except thickness is 40 μm, to opening up communication port 132 on glass tube 131, so that inside glass tube 131
Cavity is connected with the external world；Uv-curable glue is filled in the glass tube 131, and makes ultra-violet curing adhesive curing, and after making solidification
The diameter of uv-curable glue is less than the cavity diameter in glass tube 131；The uv-curable glue being more than in glass tube 131 is removed, with
The uv-curable glue inside glass tube 131 is set to reach best interference light intensity contrast；Microchannel is made on micro flow chip 107
1, and open up inlet 103 and liquid outlet 104；Glass tube 131, the first optical fiber 201 and the second optical fiber 204 are placed in microchannel
In 1, uv-curable glue is used in combination respectively to close the light inlet 11 at 1 both ends of microchannel and light-emitting window 12；First optical fiber 201 is remote
One end from glass tube 131 provides part 102 with light source and is connected, by the one end of the second optical fiber 204 far from glass tube 131 and spectrum
Instrument 106 is connected.
A kind of biosensor and preparation method thereof based on optical fiber mach-Zeng Deer interferometers provided by the invention,
Operation principle or process are as follows：When making biosensor, light guide assemblies 13 are first placed in the microchannel of micro flow chip 107
In 1, then the light inlet 11 at 1 both ends of microchannel and light-emitting window 12 are sealed with uv-curable glue, then by the first optical fiber 201
One end far from glass tube 131 provides part 102 with light source and is connected, by second one end of optical fiber 204 far from glass tube 131
It is connected with spectrometer 106, passes through the first optical fiber 201, glass tube to enable light source to provide the broadband light that part 102 provides
131 and second optical fiber 204 and be incident upon on spectrometer 106, one end of plastic flexible pipe 15 is then fixed on micro flow chip 107
At inlet 103, plastic flexible pipe 15 is set to be connected with microchannel 1 by inlet 103, finally going out in micro flow chip 107
Waste collecting device 105 is installed at liquid mouth 104, to complete the making of biosensor, therefore during making, is ensured
The safety of producer.
When using biosensor, first syringe 101 is used to extract biological tissue's solution, then by syringe 101 and modeling
Material hose 15 is connected, and injects biological group into microchannel 1 by plastic flexible pipe 15 and inlet 103 under set rate
Solution is knitted, after biological tissue's solution enters microchannel 1 and MZ chambers 202, starts light source and part 102 is provided, light source is made to provide part
102 provide broadband light, and broadband light is transmitted in the first fibre core, and when broadband light reaches MZ chambers 202, while it is molten to enter biological tissue
The reference arm that the interfere arm and filling member 203 that liquid is formed are formed, and in the second optical fiber 204 of interfere arm and reference arm end
Locate bout, since there are refringences between interfere arm and reference arm, therefore broadband light generates optical path difference, forms interference, and in width
After band light projection is on spectrometer 106, interference spectrum is formed, data are recorded after spectrum-stable, then change ethanol injection miniflow
In chip 107, microchannel 1 and MZ chambers 202 is cleaned, and the waste liquid of generation is imported in waste collecting device 105, then changed another
In the biological tissue's solution injection microchannel 1 and MZ chambers 202 of a kind of various concentration, and biological group of various concentration is tested successively
The spectrogram for knitting solution is used as by the uv-curable glue that filling refractive index is 1.36 in MZ chambers 202 and refers to arm, makes reference arm
Refractive index be closer to the refractive index of aqueous solution, to make provided by the invention to be based on optical fiber mach-Zeng Deer interferometers
Biosensor have higher detectivity.
The density of biological tissue's solution of various concentration has differences, to be had differences in terms of refractive index, and it is different
After the biological tissue solution injection MZ chambers 202 of concentration, change the refractive index of interfere arm, so as to cause interfere arm and reference arm it
Between refringence change, to make interference spectrum occur wave length shift, pass through detect interference spectrum wave length shift, energy
Enough indirect detections go out the concentration variation of measured biological tissue's solution, after using biosensor provided by the present invention,
Make the biosensor provided by the invention based on optical fiber mach-Zeng Deer interferometers have higher refractive index sensitivity and compared with
Low refractive index detection limit, therefore also show excellent sensing characteristics in terms of detection biological tissue solution concentration.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement etc., should all be included in the protection scope of the present invention made by within refreshing and principle.
Claims (10)
1. a kind of biosensor based on optical fiber mach-Zeng Deer interferometers, which is characterized in that including：Micro flow chip
(107), microchannel (1) is offered in the micro flow chip (107), the micro flow chip (107) offers inlet
(103), the inlet (103) is connected with the microchannel (1)；
The light guide assemblies (13) being arranged in the microchannel (1), the light guide assemblies (13) are interior to offer MZ chambers (202),
The MZ chambers (202) are connected with the microchannel (1)；
Light source in the micro flow chip (107) side is set, part (102) is provided, the light source provides part (102) and the light
One end of guide assembly (13) is connected；And
Spectrometer (106) in the micro flow chip (107) side, the spectrometer (106) and the light guide assemblies are set
(13) one end that part (102) is provided far from the light source is connected.
2. the biosensor according to claim 1 based on optical fiber mach-Zeng Deer interferometers, it is characterised in that：Institute
Stating light guide assemblies (13) includes：First optical fiber (201)；
Light guide in described first optical fiber (201) one end is set, cavity is offered inside the light guide, the cavity is MZ
Chamber (202)；And
It is arranged in second optical fiber (204) of the light guide far from described first optical fiber (201) one end, first optical fiber
(201) and second optical fiber (204) is symmetrical arranged along the light guide.
3. according to the biosensor based on optical fiber mach-Zeng Deer interferometers described in claim 2, it is characterised in that：It is described
Light guide includes：
Glass tube (131), the opposite end of the glass tube (131) respectively with first optical fiber (201) and second light
Fine (204) phase welding, glass tube (131) side offer communication port (132), communication port (132) both ends respectively to
First optical fiber (201) and second optical fiber (204) extend, and extend to the opposite both ends of the glass tube (131), institute
MZ chambers (202) are stated by the communication port (132) to be connected with the external world；And it is filled in the filling member in MZ chambers (202)
(203), the diameter of the filling member (203) is less than the diameter of the MZ chambers (202), first optical fiber (201) and described the
One end that two optical fiber (204) are fused to the glass tube (131) is respectively positioned on the inner wall of the cavity, and part and the filling
Part (203) is in contact.
4. the biosensor according to claim 3 based on optical fiber mach-Zeng Deer interferometers, it is characterised in that：Institute
It is uv-curable glue to state filling member (203), and the refractive index of the uv-curable glue is 1.36.
5. the biosensor according to claim 3 based on optical fiber mach-Zeng Deer interferometers, it is characterised in that：Institute
It is 100 μm to state glass tube (131) length, and glass tube (131) outer diameter and internal diameter are respectively 125 μm and 75 μm.
6. the biosensor according to claim 2 based on optical fiber mach-Zeng Deer interferometers, it is characterised in that：Institute
It is single mode optical fiber fibre core to state the first optical fiber (201) and second optical fiber (204).
7. the biosensor according to claim 1 based on optical fiber mach-Zeng Deer interferometers, it is characterised in that：Institute
It states light source and provides part (102) for providing wideband light source.
8. the biosensor according to claim 1 based on optical fiber mach-Zeng Deer interferometers, it is characterised in that：Institute
It states and is provided with plastic flexible pipe (15) at the inlet (103) that micro flow chip (107) opens up.
9. the biosensor according to claim 1 based on optical fiber mach-Zeng Deer interferometers, it is characterised in that：Institute
It states micro flow chip (107) and offers liquid outlet (104), the liquid outlet (104) is connected with the microchannel (1), described
Micro flow chip (107) side is provided with waste collecting device (105), the waste collecting device (105) and the liquid outlet
(104) it is connected.
10. a kind of production method of the biosensor based on optical fiber mach-Zeng Deer interferometers, which is characterized in that the side
Method includes：
Cutting process is done by one end phase welding of the first optical fiber (201) and glass tube (131), and to glass tube (131), is then existed
The second optical fiber of other end welding (204) of glass tube (131)；
One side throwing of glass tube (131) is removed, to open up communication port (132) on glass tube (131), so that glass tube
(131) internal cavity is connected with the external world；
Uv-curable glue is filled in glass tube (131), and makes ultra-violet curing adhesive curing, and makes the uv-curable glue after solidification
Diameter is less than the cavity diameter in glass tube (131)；
Uv-curable glue extra in glass tube (131) is removed, so that the uv-curable glue of glass tube (131) inside reaches best
Interference light intensity contrast；
Microchannel (1) is made on micro flow chip (107), and opens up inlet (103) and liquid outlet (104)；
Glass tube (131), the first optical fiber (201) and the second optical fiber (204) are placed in microchannel (1), ultra-violet curing is used in combination
Glue respectively closes the light inlet (11) at microchannel (1) both ends and light-emitting window (12)；
The one end of the first optical fiber (201) far from glass tube is provided to part (102) with light source to be connected, the second optical fiber (204) is remote
One end from glass tube (131) is connected with spectrometer (106).
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020014858A1 (en) * | 2018-07-17 | 2020-01-23 | 深圳大学 | Fiber mach-zehnder interferometer-based biosensor and production method therefor |
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| EP1065539A2 (en) * | 1999-06-23 | 2001-01-03 | Thomas & Betts International, Inc. | Optical fiber mach-zehnder interferometer employing miniature bends |
| CN101451959A (en) * | 2008-12-30 | 2009-06-10 | 清华大学 | Hydrogen sensor and pd film hydrogen sensing system |
| US20110102803A1 (en) * | 2009-11-02 | 2011-05-05 | The Hong Kong Polytechnic University | In-line single fiber mach-zehnder interferometer |
| CN107063552A (en) * | 2016-11-30 | 2017-08-18 | 深圳市中葛科技有限公司 | A kind of fiber-optic fiber gas pressure sensor |
| CN106645029A (en) * | 2016-12-07 | 2017-05-10 | 大连理工大学 | Weld type open-cavity optical fiber EFPI (extrinsic Farby-Perot interferometric) refractive index sensor |
| CN107314960A (en) * | 2017-08-15 | 2017-11-03 | 深圳大学 | A kind of hematocrite concentration sensor and preparation method thereof, test device |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020014858A1 (en) * | 2018-07-17 | 2020-01-23 | 深圳大学 | Fiber mach-zehnder interferometer-based biosensor and production method therefor |
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