CN108676745A - Solution xylose lysine bacillus LX024 and its application of one plant of prevention wheat base rot disease and head blight - Google Patents
Solution xylose lysine bacillus LX024 and its application of one plant of prevention wheat base rot disease and head blight Download PDFInfo
- Publication number
- CN108676745A CN108676745A CN201810493252.0A CN201810493252A CN108676745A CN 108676745 A CN108676745 A CN 108676745A CN 201810493252 A CN201810493252 A CN 201810493252A CN 108676745 A CN108676745 A CN 108676745A
- Authority
- CN
- China
- Prior art keywords
- rot disease
- base rot
- head blight
- wheat
- disease
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 69
- 201000010099 disease Diseases 0.000 title claims abstract description 68
- 241000209140 Triticum Species 0.000 title claims abstract description 66
- 235000021307 Triticum Nutrition 0.000 title claims abstract description 66
- 241000193830 Bacillus <bacterium> Species 0.000 title claims abstract description 27
- GBWARTHIRIVTNI-PJHQGUKWSA-N (2s)-2,6-diaminohexanoic acid;(2r,3s,4r)-2,3,4,5-tetrahydroxypentanal Chemical compound NCCCC[C@H](N)C(O)=O.OC[C@@H](O)[C@H](O)[C@@H](O)C=O GBWARTHIRIVTNI-PJHQGUKWSA-N 0.000 title claims abstract description 25
- 230000002265 prevention Effects 0.000 title claims abstract description 21
- 241000196324 Embryophyta Species 0.000 title claims description 17
- 239000012681 biocontrol agent Substances 0.000 claims abstract description 28
- 230000001580 bacterial effect Effects 0.000 claims abstract description 9
- 241001589484 Lysinibacillus xylanilyticus Species 0.000 claims abstract description 6
- 238000004321 preservation Methods 0.000 claims abstract description 5
- 241000894006 Bacteria Species 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 13
- 238000012545 processing Methods 0.000 claims description 10
- 244000005700 microbiome Species 0.000 claims description 8
- 238000011081 inoculation Methods 0.000 claims description 7
- 239000002068 microbial inoculum Substances 0.000 claims description 6
- 241000607479 Yersinia pestis Species 0.000 claims description 3
- 230000003628 erosive effect Effects 0.000 claims description 3
- 230000003449 preventive effect Effects 0.000 abstract description 13
- 230000000694 effects Effects 0.000 abstract description 8
- 239000007788 liquid Substances 0.000 abstract description 2
- 238000009629 microbiological culture Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 25
- 206010039509 Scab Diseases 0.000 description 12
- 230000000443 biocontrol Effects 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 7
- 239000000126 substance Substances 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 5
- 108020004465 16S ribosomal RNA Proteins 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 244000052769 pathogen Species 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 241000223195 Fusarium graminearum Species 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 238000011835 investigation Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000001717 pathogenic effect Effects 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
- 239000004472 Lysine Substances 0.000 description 2
- 239000012620 biological material Substances 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- 230000003020 moisturizing effect Effects 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 241000192125 Firmicutes Species 0.000 description 1
- 241000726221 Gemma Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 101100460844 Mus musculus Nr2f6 gene Proteins 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 208000035199 Tetraploidy Diseases 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 230000009418 agronomic effect Effects 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- 238000004500 asepsis Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- 239000003124 biologic agent Substances 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000011217 control strategy Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 208000010801 foot rot Diseases 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000004570 mortar (masonry) Substances 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 239000002420 orchard Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Environmental Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- General Engineering & Computer Science (AREA)
- Virology (AREA)
- Forests & Forestry (AREA)
- Ecology (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Medicinal Chemistry (AREA)
- Toxicology (AREA)
- Pest Control & Pesticides (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Botany (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention discloses a kind of solution xylose lysine bacillus LX024 and its application of prevention wheat base rot disease and head blight.The bacterial strain is solution xylose lysine bacillus（Lysinibacillus xylanilyticus）, it is preserved in China General Microbiological culture presevation administrative center, preservation date September in 2017 19 days, deposit number is CGMCC No.14645.The biocontrol agent made of LX024 strain liquid fermented and cultureds has better effects to prevention wheat base rot disease and head blight.There are a degree of control effect, preventive effect to respectively reach 65.01% and 65.81% prevention wheat base rot disease and head blight using biocontrol agent LX024 in Wheat Seedling and growth period.
Description
Technical field
The invention belongs to field of agricultural microorganism, are related to one plant of biological and ecological methods to prevent plant disease, pests, and erosion solution for preventing wheat base rot disease and head blight
Xylose lysine bacillus LX024 and its application.
Background technology
Wheat scab and base rot disease are seriously affected by the microbial global disease for endangering wheat of F.graminearum schw
The yield and quality of wheat.Wherein wheat base rot disease be it is a kind of invaded by a variety of pathogens, the one kind for mixing generation compound is invaded
Metachromia soil-borne disease, also referred to as reaping hook root rot and reaping hook base rot disease etc..The disease is a kind of global important disease,
There is more than 10 country to report it.In China, the harm of Disease of Wheatbrown Foot Rot was once reported in Jiangsu, big according to Henan Agriculture
The investigation of wheat soil-borne diseases seminar of plant protection institute is learned, which generally sends out in China the Yellow River and Huai He River wheat main producing region each province
It is raw.Wheat scab is caused by Fusarium graminearum synthesis species, is happened at warm moist and one kind of semi-humid region extensively
Destructive disease.The Yangtze River in China middle and lower reaches area of wheat and the Major Epidemic area that eastern part of Northeast spring wheat area is the disease.Recently as complete
The morbidity range of ball climate change, wheat scab has spread to varieties in Huang-Huai-Hai Plain south.Illness wheat seriously affects wheat production
Amount and quality also jeopardize people, animal health.
Currently, mainly taking chemical prevention to the disease control of wheat base rot disease and head blight, breeding resistant variety, adding
The measures such as strong field management.But since breeding cycle is long and the limitation of field management, chemical prevention is most common prevention
Method.Though chemical prevention can effectively control the development of the field state of an illness, secondary dirt is easily caused to ecological environment and Wheat Production
Dye, therefore, environmentally friendly biological prevention gradually draws attention.Biological control strategy has biological control source resource
Extensively, the features such as environmentally friendly, every country and area scholar and researcher all try to explore and assess biology prevent
Control application prospect and potential value of the method on Fusarium graminearum means of prevention.Therefore biological control is red as novel wheat
Mildew and base rot disease control measure are worth with good production application.
Invention content
The purpose of the present invention is for the life not having to wheat scab and base rot disease in the prior art compared with high preventive effect
The present situation of anti-preparation, provide it is a kind of prevention wheat base rot disease and head blight solution xylose lysine bacillus LX024 and its
Using.
The purpose of the present invention can be achieved through the following technical solutions：
The solution xylose lysine bacillus LX024 of one plant of prevention wheat base rot disease and head blight, the identified Pseudomonas is in solution
Xylose lysine bacillus LX024（Lysinibacillus xylanilyticus）, the biological material specimens preservation
And it survives, biomaterial Classification And Nomenclature is solution xylose lysine bacillus Lysinibacillus xylanilyticus,
The deposit date is Septembers in 2017 19 days, and depositary institution is China Committee for Culture Collection of Microorganisms's common micro-organisms center
（CGMCC）, depositary institution address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, and culture presevation number is CGMCC
No.14645。
Above-mentioned solution xylose lysine bacillus LX024 is in prevention wheat base rot disease and head blight or prepares for preventing
Control the application in wheat base rot disease and the biocontrol agent of head blight.
It is a kind of to be used to prevent wheat base rot disease and the biocontrol agent of head blight, above-mentioned solution wood is included in the biocontrol agent
Sugared lysine bacillus LX024.As a kind of optimal technical scheme, viable bacteria total concentration in the biocontrol agent not less than 1 ×
108 CFU/mL。
The preparation method of the microbial inoculum is：By the seed bacterium solution of above-mentioned solution xylose lysine bacillus LX024 with 1:500 bodies
Product is than being seeded to fermented and cultured in LB fermentation mediums, 28 DEG C of 150 ~ 200 40 ~ 48 h of rpm shaken cultivations, then 5000 ~
6000 rpm centrifuge 10 ~ 15min, take and precipitate up to the biocontrol agent LX024, are diluted with aqua sterilisa and microbial inoculum is made,
Viable bacteria total concentration is 1 × 10 in finished product microbial inoculum8~1×109 CFU/mL。
Certainly, the solution xylose lysine bacillus LX024 systems of the prevention wheat scab and base rot disease are utilized
Standby biocontrol agent is not limited to above method.It is every can mass propgation LX024, and keep the active method of its biological and ecological methods to prevent plant disease, pests, and erosion available
In the claimed biocontrol agent of the preparation present invention.
The preparation for the solution xylose lysine bacillus LX024 seed bacterium solutions that the preserving number is CGMCC No. 14645
Method is：The solution xylose lysine bacillus LX024 inoculations that preserving number is CGMCC No. 14645 are cultivated to LB
OD values in liquid at 28 DEG C of 180 rpm shaken cultivations to 600 nm of culture terminate to cultivate when being 0.8.
A method of prevention wheat base rot disease and head blight carry out at inoculation wheat with the biocontrol agent
Reason.
Wheat is inoculated under greenhouse and crop field environment respectively after the biocontrol agent is diluted 100-200 times
Processing, when morbidity, start to count the state of an illness.Solve preventive effects of the xylose lysine bacillus LX024 to wheat base rot disease and head blight
Respectively 65.01 and 65.81%.
Beneficial effects of the present invention：
The present invention is specific to the biological prevention and control agent of wheat scab and base rot disease exploitation.Due to being biological agent, do not have completely
There is a series of problems caused by the use because of chemical pesticide, thus be conducive to the No-harmful apple orchard of wheat, peasant can not have to
Or the dosage of other chemical pesticides is reduced, this can not only reduce expenses for peasant, but also reduce the residual of chemical agent.
Greenhouse experiments and field experiment show：The solution xylose lysine bacillus LX024（CGMCC No. 14645）Energy
Significantly reduce the generation of wheat scab and base rot disease.The biocontrol agent prepared using the bacterium is diluted 100 ~ 200 times and is filled
Root processing, to the preventive effect of wheat base rot disease and head blight respectively up to 65.01 and 65.81%.
Biological sample preservation information
One plant of solution xylose lysine bacillus LX024, Classification And Nomenclature areLysinibacillus xylanilyticus, in
September is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on 19th within 2017（CGMCC）, preservation address
For Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, culture presevation number is CGMCC No.
14645。
Specific implementation mode
Embodiment 1
Screening technique：Solve xylose lysine bacillus LX024（CGMCC No. 14645）It is small by Jiangsu Province Huai'an harbour town
It detaches and obtains in wheat rhizosphere soil.Wheat root is cut into the small pieces of 1cm, impregnates 5 min, the leaching of 70% alcohol with 1% sodium hypochlorite
Steep 1-2 min, sterile water wash 3 times（It takes the 100 μ l of solution that last time is cleaned to be applied on R2A solid mediums to cultivate, if
Asepsis growth then thinks that surface sterilization is clean after 48 h, sterile）.It takes the sample that 3 g are disinfected to be placed in bacterium mortar, is added 27
0.85% sterile ml NaCl macerates tissue, grinding, with 0.85% sterile NaCl gradient dilutions.Take 10-1,10-2,
Each 100 μ l of dilution of 10-3 three gradients are applied on R2A, 28 DEG C of 48 h of culture.The maximum single bacterium colony of picking is inoculated into newly
Fresh R2A solid mediums obtain purifying bacterial strain through transferring repeatedly.By bacterial strain after purification with 40% glycerine be stored in -70 DEG C it is ultralow
In temperature refrigerator.
Identification method：By morphological feature, the bacterial strain is identified in bio-chemical characteristics and 16S rDNA sequence analyses.
Morphological feature：Gram-positive bacteria, it is rod-shaped, gemma is generated, bacterium colony is circle, and faint yellow, surface is smooth.
Gene magnification and sequence analysis:Xylose lysine bacillus LX024 is solved to cultivate extremely for 28 DEG C in LB culture mediums
Logarithmic phase centrifuges 5 min with 12 000 r/min and collects thalline, using precious bioengineering（Dalian）The genome of Co., Ltd
DNA rapid extraction kits, extract the genomic DNA of bacterium, using the DNA product of extraction as template, are expanded with bacterial 16 S rRNA
Increase universal primer U8-27 (5 '-AGAGTTTGATC (AC) TGGCTCAG-3 '), L1494-1514 (5 '-CTACGG (AG)
TACCTTGTTACGAC-3 ') 16S rDNA genetic fragments are amplified from genomic DNA.PCR product serves the raw work biology in sea
Engineering Co., Ltd is sequenced.By BLAST softwares tetraploid rice, knot are carried out to measuring 16S rRNA gene orders
Therefore the bacterial strain is accredited as solution xylose lysine bacillus by fruit abridged table（Lysinibacillus xylanilyticus）.
Comparison result is sequenced in 1 LX024 of table
| Bacterial strain | Most like bacterial strain and accession number | Similarity (%) |
| LX024 | Lysinibacillus xylanilyticus KX703024.1 | 99 |
The preparation of 2 LX024 biocontrol agents of embodiment
It will solution xylose lysine bacillus LX024（CGMCC No. 14645）28 DEG C 180 in inoculation to LB culture solutions
It after 16 h of rpm shaken cultivations, is sampled in superclean bench every 2 h and surveys its OD value at 600 nm, when OD values are 0.8
When terminate to cultivate, this bacterium solution is as seed bacterium solution.Seed bacterium solution with 1:500 volume ratios, which are seeded in LB fermentation cultures, ferments
Culture, 28 DEG C, 180 rpm, 48 h of culture, then 6000 rpm centrifugations, 10 min, take and precipitate up to the biocontrol agent
LX024.It is diluted biocontrol agent LX024 with aqua sterilisa, viable bacteria total concentration is 1 × 10 in gained biocontrol agent8 ~ 1×
10 9 CFU/mL。
The preventive effect of 3 greenhouse of embodiment and plot experiment biocontrol microorganisms LX024 to wheat scab and base rot disease
Experiment 1：Greenhouse test detects preventive effects of the LX024 to wheat base rot disease（2016.09-2016.12）
Place：Huaiyingong College gives birth to academy of sciences greenhouse
Greenhouse pot culture is tested：Wheat seed is first used to aseptic water washing twice, repeats to use after being sterilized within two minutes with 75% alcohol washes
Aseptic water washing three times, the wheat seed after disinfection cultivate germination in culture dish.In sowing to basin alms bowl, two leaves are grown to wholeheartedly
Phase is inoculated with biocontrol bacteria.It is inoculated with pathogen after a week, observation statistics is carried out after 7d one month.Greenhouse（25 DEG C of temperature）It cultivates small
Wheat seeding is per three plants of basin, and in two one heart stages of leaf, biocontrol microorganisms LX024 bacterium solutions and control group root irrigation are each to handle in triplicate,
It is inoculated with pathogen after 7d, takes moisturizing 48h, greenhouse-grown after inoculation at 25 DEG C.Daily to wheat basal part of stem base rot disease into
Row observation survey.
Wheat base rot disease investigates foundation《Wheat scab Forecast Techniques specification》(GB/T 15796-2011) combines this reality
The actual conditions tested record and analyze test data.
0 grade, outer layer leaf sheath non-evident sympton；
1 grade, the first leaf sheath it is brown it is withered less than leaf sheath length 1/4；
2 grades, the first leaf sheath it is brown it is withered less than leaf sheath length 1/4-1/2；
3 grades, the first leaf sheath it is brown it is withered less than leaf sheath length 1/2-3/4；
4 grades, the first leaf sheath whole removing green is rotted；
5 grades, third leaf sheath has apparent brown withered or complete stool withered,
Disease severity (disease index)=∑（Sick series × disease grade plant the number）/（Highest disease series × total plant number）×
100%
Biocontrol effect=（Control group disease severity-processing group disease severity）/ control group disease severity × 100%
Note：Calculate different disposal disease index and preventive effect, and using DPS Data Analysis Software progress significance difference analysis and
Multiple range test.
Investigation result after biocontrol agent LX024 is handled 15 days shows that biocontrol agent LX024 is to wheat base rot disease
Biological control effect（Hereinafter referred to as preventive effect）Reach 65.01%（Table 2）.
Biocontrol effects of the 2 biocontrol agent LX024 of table to wheat base rot disease
| Processing | Disease severity % | Preventive effect % |
| LX024 microbial inoculums | 21.57±2.33b | 65.01 |
| Control | 61.63±3.05a | --- |
Experiment 2：Field plot trial detects preventive effects of the LX024 to wheat scab（2016.10-2017.05）
Place：Huaiyingong College agronomic trials field
Experimental field preceding stubble is corn, and the soil texture is sandy loam.On October 24th, 2014：Wheat cultivation, thickness of sowing 100
Grain m-1, line space 25cm applies seed manure between two every trades（Composite fertilizer）10g；On November 14th, 2014：Wheat thinning, thinning is extremely
70-80 plants of m-1；November 23：Tillering fertilizer is applied, urea 5g is in the ranks applied.Other management are according to Huai'an high-yield culturing mode
It carries out.
Experimental plot 15m × 10m=150m2, experimental plot surrounding plantation protection row.2 processing of this experiment, each handle 4 weights
It is multiple.
This experiment selects wheat breed for Huaihe River wheat 27；Gibberellic hypha Inoculating date is jan flowering wheat, carries out single flower inoculation
Inoculation, each kind is not fallen ill before being inoculated with, and 2 little Hua of middle part small ear are inoculated with per fringe, and each cell that handles is inoculated with 20 fringes, connects
It is inoculated with pathogen after kind biocontrol microorganisms LX024 and moisturizing 1d；After 21d, each cell that handles samples 10 plants.It compares and each
Processing weighs 4 times.
3 wheat scab disease severity of table is classified
| Severity gradation | Head blight Disease symptoms describe |
| 0 | Fringe portion is without clearly visible disease symptom |
| 1 | Only it is inoculated with small ear or the morbidity of adjacent small ear |
| 2 | Morbidity small ear accounts for the 1/4 or less of spike length |
| 3 | Morbidity small ear accounts for the 1/4-1/2 of spike length |
| 4 | Morbidity small ear accounts for 1/2 of spike length or more |
Disease severity (disease index)=∑（Sick series × disease grade plant the number）/（Highest disease series × total plant number）×
100%
Biocontrol effect=（Control group disease severity-processing group disease severity）/ control group disease severity × 100%
Note：Calculate different disposal disease index and preventive effect, and using DPS Data Analysis Software progress significance difference analysis and
Multiple range test.
Investigation result after the processing 35 days of biocontrol agent LX024 the 1st time shows that biocontrol agent LX024 is to wheat
The biological control effect of head blight（Hereinafter referred to as preventive effect）Reach 65.81%（Table 4）.
Biocontrol effects of the 4 biocontrol agent LX024 of table to wheat scab
| Processing | Disease severity % | Preventive effect % |
| LX024 microbial inoculums | 20.94±1.56b | 65.81 |
| Control | 61.24 ±1.43a | --- |
Claims (7)
1. the solution xylose lysine bacillus LX024 of one plant of prevention wheat base rot disease and head blight, the identified bacterial strain category
In solution xylose lysine bacillus LX024（Lysinibacillus xylanilyticus）, the preservation on the 19th of September in 2017
In China Committee for Culture Collection of Microorganisms's common micro-organisms center, culture presevation number is CGMCC No.14645.
2. solution xylose lysine bacillus LX024 described in claim 1 is in prevention wheat base rot disease and head blight
Using.
3. solution xylose lysine bacillus LX024 described in claim 1 is being prepared for preventing wheat base rot disease and red
Application in the biocontrol agent of mildew.
4. a kind of for preventing wheat base rot disease and the biocontrol agent of head blight, which is characterized in that include in the biocontrol agent
Solution xylose lysine bacillus LX024 described in claim 1.
5. according to claim 4 for preventing wheat base rot disease and the biocontrol agent of head blight, which is characterized in that should
Viable bacteria total concentration in biocontrol agent is not less than 1 × 108 CFU/mL。
6. application of the biocontrol agent in prevention wheat base rot disease and head blight described in claim 4 or 5.
7. a kind of method of prevention wheat base rot disease and head blight, which is characterized in that with the biological and ecological methods to prevent plant disease, pests, and erosion described in claim 4 or 5
Microbial inoculum carries out inoculation processing to wheat.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810493252.0A CN108676745B (en) | 2018-05-22 | 2018-05-22 | Lysine bacillus xylolyticus LX024 for preventing and treating wheat stem basal rot and gibberellic disease and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810493252.0A CN108676745B (en) | 2018-05-22 | 2018-05-22 | Lysine bacillus xylolyticus LX024 for preventing and treating wheat stem basal rot and gibberellic disease and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108676745A true CN108676745A (en) | 2018-10-19 |
| CN108676745B CN108676745B (en) | 2021-04-13 |
Family
ID=63807652
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810493252.0A Active CN108676745B (en) | 2018-05-22 | 2018-05-22 | Lysine bacillus xylolyticus LX024 for preventing and treating wheat stem basal rot and gibberellic disease and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108676745B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111996125A (en) * | 2020-08-18 | 2020-11-27 | 河南省农业科学院植物保护研究所 | Application of chaetomium globosum in preventing and treating wheat stem basal rot |
| CN113925064A (en) * | 2021-11-01 | 2022-01-14 | 海南大学 | Application of B-lysine-resistant bacillus in inhibition of growth of pitaya soft rot germs |
| CN114134087A (en) * | 2021-12-22 | 2022-03-04 | 安徽农业大学 | Streptomyces algicidal and application thereof in prevention and treatment of wheat stem basal rot |
| CN114908012A (en) * | 2022-05-19 | 2022-08-16 | 中国水产科学研究院黑龙江水产研究所 | Bacterial strain beneficial to symbiosis of rice and fish and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106748424A (en) * | 2016-11-30 | 2017-05-31 | 浙江科技学院 | Bamboo grove special bio charcoal base manure material, preparation method and applications |
-
2018
- 2018-05-22 CN CN201810493252.0A patent/CN108676745B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106748424A (en) * | 2016-11-30 | 2017-05-31 | 浙江科技学院 | Bamboo grove special bio charcoal base manure material, preparation method and applications |
Non-Patent Citations (1)
| Title |
|---|
| ZAKIRA NAUREEN ET AL.: "Exploring the Potentials of Lysinibacillus sphaericus ZA9 for Plant Growth Promotion and Biocontrol Activities against Phytopathogenic Fungi", 《FRONTIERS IN MICROBIOLOGY》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111996125A (en) * | 2020-08-18 | 2020-11-27 | 河南省农业科学院植物保护研究所 | Application of chaetomium globosum in preventing and treating wheat stem basal rot |
| CN111996125B (en) * | 2020-08-18 | 2022-03-04 | 河南省农业科学院植物保护研究所 | Application of chaetomium globosum in preventing and treating wheat stem basal rot |
| CN113925064A (en) * | 2021-11-01 | 2022-01-14 | 海南大学 | Application of B-lysine-resistant bacillus in inhibition of growth of pitaya soft rot germs |
| CN114134087A (en) * | 2021-12-22 | 2022-03-04 | 安徽农业大学 | Streptomyces algicidal and application thereof in prevention and treatment of wheat stem basal rot |
| CN114134087B (en) * | 2021-12-22 | 2023-07-14 | 安徽农业大学 | Streptomyces alginolyticus and application thereof in prevention and control of wheat stem basal rot |
| CN114908012A (en) * | 2022-05-19 | 2022-08-16 | 中国水产科学研究院黑龙江水产研究所 | Bacterial strain beneficial to symbiosis of rice and fish and application thereof |
| CN114908012B (en) * | 2022-05-19 | 2022-10-28 | 中国水产科学研究院黑龙江水产研究所 | Bacterial strain beneficial to symbiosis of rice and fish and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108676745B (en) | 2021-04-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105886428B (en) | One plant of Streptomycesalbidoflhaving and its application in microbial manure | |
| CN105296381B (en) | One bacillus subtilis CYY-25 and its application | |
| CN105543132B (en) | A kind of Methylotrophic bacillus YB-F7 and its application in controlling plant diseases | |
| CN108676745A (en) | Solution xylose lysine bacillus LX024 and its application of one plant of prevention wheat base rot disease and head blight | |
| CN103146586A (en) | Trichoderma harzianum strain for controlling plant fungus diseases and application thereof | |
| CN106399129A (en) | Trichoderma harzianum strain and application thereof | |
| CN105132296B (en) | A kind of hook-shaped trichoderma strain and its application | |
| CN105154339B (en) | A kind of Trichoderma viride bacteria strain and its application | |
| CN109112069B (en) | Biocontrol endophytic fungus and application thereof | |
| CN103642734A (en) | Microbacterium maritypicum and application thereof in preventing sugar beet disease-causing organisms | |
| CN101215543A (en) | Strain for preventing and curing muskmelon bacteroidal spot disease and application thereof in field disease prevention | |
| CN106701631A (en) | Strain of streptomyces roseoflavus and application thereof | |
| CN109749953B (en) | Bacillus cereus, microbial inoculum and preparation method and application thereof | |
| CN110317747A (en) | A kind of bacillus amyloliquefaciens JT68 and its application in prevention and treatment tea anthracnose | |
| CN111334458B (en) | Biocontrol actinomycetes and application thereof in prevention and control of ginger stem basal rot or soybean epidemic disease | |
| CN111548948B (en) | Microbial agent JF for preventing and treating stem rot of corn in saline-alkali soil and preparation method thereof | |
| CN110205249B (en) | Method for promoting plant growth and alternaria alternate fungus used by same | |
| CN109609403B (en) | Biocontrol bacterium and application thereof in prevention and control of downy mildew of crops | |
| CN105316258B (en) | It is a kind of prevent and treat rice sheath blight disease bacterial strain 1LN2 and its application | |
| CN104593284B (en) | One plant of Endophyte of walnut and its application | |
| CN111454848B (en) | Actinomucor elegans GD48, fermentation product, microbial inoculum and application thereof | |
| CN111321094B (en) | Microbial agent M1 for preventing and treating stem basal rot of corn and application thereof | |
| CN109749938A (en) | The endogenetic fungus and its microbial inoculum of one plant of reduction notoginseng root rot disease incidence | |
| CN110260976B (en) | Method for obtaining applied dose of microorganism AM and DSE fungus combined microbial inoculum by thermal infrared monitoring | |
| CN114276938A (en) | Paecilomyces EJKS strain, fusarium E-9 strain and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20231215 Address after: 271200 No. 512 Binhe Avenue, Xintai Economic Development Zone, Tai'an City, Shandong Province Patentee after: Zhongke Xinrun (Shandong) Agricultural Technology Development Co.,Ltd. Address before: 223005 Huaian 1 Jiangsu Economic Development Zone Patentee before: HUAIYIN INSTITUTE OF TECHNOLOGY |
|
| TR01 | Transfer of patent right |