CN108636469A - A kind of contactless liquid-transfering device and liquid relief method - Google Patents
A kind of contactless liquid-transfering device and liquid relief method Download PDFInfo
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- CN108636469A CN108636469A CN201810614967.7A CN201810614967A CN108636469A CN 108636469 A CN108636469 A CN 108636469A CN 201810614967 A CN201810614967 A CN 201810614967A CN 108636469 A CN108636469 A CN 108636469A
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- liquid
- transfering
- solenoid valve
- translation component
- pressure control
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
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- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention discloses a kind of contactless liquid-transfering devices, including reaction plate, liquid relief module, translation component and control module, translation component is fixed at the top of liquid relief position, reaction plate is placed on liquid relief position, the liquid relief end of liquid relief module is fixedly connected with the mobile terminal of translation component, and control assembly is connect with liquid relief module；Liquid relief method includes that liquid-transfering needle group is moved to imbibition position, it will be in liquid inhalation solution tank and pressure control vessel, translation component at the uniform velocity moves, adjust the open frequency of high-frequency electromagnetic valve, its interval time is the shifting speed ratio of the distance between reacting hole group and translation component, and liquid sprays into reacting hole from liquid-transfering needle.The present invention controls the duration of valve opening of liquid-transfering needle group pipeline by high-frequency electromagnetic valve, can precisely adjust the amount that liquid-transfering needle sprays liquid every time, realizes ultramicron hydrojet；High-frequency electromagnetic valve is switched in liquid-transfering needle group uniform motion according to the frequency of setting, and the liquid that liquid-transfering needle sprays is made accurately to spray into reacting hole, realizes non-contact accurate liquid relief.
Description
Technical field
The present invention relates to experimental provision more particularly to a kind of contactless liquid-transfering device and liquid relief methods.
Background technology
With the development of science and technology, the liquid relief during liquid dosage and cleaning realized substantially it is semi-automatic or
Automation, with pure manual liquid dosage process compare, it can be achieved that automatic sample, the reagent of sample distribute, micropipette and with
The operations such as the relevant vibration of liquid handling, incubation, improve confecting efficiency.It can be widely applied to nucleic acid purification, gene protein
The fields such as sequencing, clone quick screening, cell culture, biochip sample preparation.But existing liquid-transfering device is simple in structure, work(
Can be single, precision and stability is not high, can not accomplish ultramicron liquid relief in pipetting processes, and may be in pipetting processes
Reagent, which is in contact, to be polluted, and final experimental result is influenced.
Invention content
The purpose of the present invention is that provides a kind of contactless liquid-transfering device and liquid relief side to solve the above-mentioned problems
Method.
The present invention is achieved through the following technical solutions above-mentioned purpose：
A kind of contactless liquid-transfering device, including reaction plate, liquid relief module, translation component and control module, the translation
Component is fixed at the top of liquid relief position, and the reaction plate is placed on the liquid relief position, the liquid relief of the liquid relief module
The mobile terminal with the translation component is held to be fixedly connected, the control assembly is connect with the liquid relief module；
The liquid relief module includes liquid-transfering needle group, NaOH solution tank NaOH, suction pumps, pulsometer and pressure control vessel, the solution
Tank is connected to the liquid-transfering needle group, the suction pumps and the pressure control vessel respectively, the pressure control vessel with it is described
Pulsometer is connected to；
The control module includes high-frequency electromagnetic valve, the first solenoid valve, second solenoid valve, third solenoid valve and proportioning valve,
The high-frequency electromagnetic valve is arranged in the fluid path between the liquid-transfering needle group and the NaOH solution tank NaOH, and the first solenoid valve setting exists
In fluid path between the NaOH solution tank NaOH and the pressure control vessel, second solenoid valve setting the suction pumps with it is described
In fluid path between NaOH solution tank NaOH, the fluid path between the suction pumps and the pressure control vessel is arranged in the third solenoid valve
On, the proportioning valve is arranged in the gas circuit between the pressure control vessel and the pulsometer.
Specifically, multiple reacting hole groups are provided on the reaction plate, the reacting hole group includes multiple linear distributions
Reacting hole, and the distance between two adjacent reacting hole groups are equal, the reacting hole group and the translation component movement side
To vertical；
The liquid-transfering needle group includes multiple linear distributing liquid-transfering needles, and the position of multiple liquid-transfering needles is reacted with described
The position in hole corresponds, and the liquid-transfering needle group is fixed on the mobile terminal of the translation component, and with the translation component
Moving direction is vertical.
Preferably, the translation component is hydraulic stem, atmospheric pressure pole or linear motor.
Preferably, the pressure control vessel lower part is solution, top is air, and the pulsometer is controlled with the pressure
The top of container is connected to, and the top of the pressure control vessel is connected with exhaust head, and the exhaust head holds with pressure control
The 4th solenoid valve is provided in gas circuit between device.
A kind of liquid relief method based on above-mentioned contactless liquid-transfering device, the liquid relief method include imbibition flow and hydrojet
Flow specifically includes following steps：
S1, the first solenoid valve are closed, and high-frequency electromagnetic valve, second solenoid valve, third solenoid valve and proportioning valve are opened；
S2, a certain amount of air of sucking；
S3, liquid-transfering needle group is moved to imbibition position, it will be in liquid inhalation solution tank and pressure control vessel；
S4, by reaction plate as liquid relief position, liquid-transfering needle group is moved to the top of reaction plate；
S5, translation component at the uniform velocity move, and second solenoid valve and third solenoid valve are closed, and the first solenoid valve and proportioning valve are opened
It opens；
S6, the open frequency for adjusting high-frequency electromagnetic valve, interval time are the distance between reacting hole group and translation component
Shifting speed ratio, liquid from liquid-transfering needle spray into reacting hole.
The beneficial effects of the present invention are：
A kind of contactless liquid-transfering device of the present invention and liquid relief method control liquid-transfering needle group pipeline by high-frequency electromagnetic valve
Duration of valve opening, pulsometer control whole system pipeline inner pressure, and liquid-transfering needle sprays under pipeline inner pressure effect in duration of valve opening
A certain amount of liquid, pipeline inner pressure are precisely controlled by proportioning valve, and combine the duration of valve opening of corresponding high-frequency electromagnetic valve, can
The amount that liquid-transfering needle sprays liquid every time is precisely adjusted, realizes ultramicron hydrojet；Liquid-transfering needle group is away from the upper of reaction plate certain distance
Square uniform motion, high-frequency electromagnetic valve are switched in liquid-transfering needle group uniform motion according to the frequency of setting, and liquid-transfering needle is made to spray
Liquid accurately spray into each reacting hole of reaction plate, realize non-contact accurate liquid relief.
Description of the drawings
Fig. 1 is a kind of structural schematic diagram of contactless liquid-transfering device of the present invention.
Specific implementation mode
The invention will be further described below in conjunction with the accompanying drawings：
As shown in Figure 1, a kind of contactless liquid-transfering device of the present invention, including reaction plate, liquid relief module, translation component and control
Molding block, translation component are fixed at the top of liquid relief position, and reaction plate is placed on liquid relief position, the liquid relief end of liquid relief module
It is fixedly connected with the mobile terminal of translation component, control assembly is connect with liquid relief module；
Liquid relief module includes liquid-transfering needle group 1, NaOH solution tank NaOH 2, suction pumps 3, pulsometer 5 and pressure control vessel 4, NaOH solution tank NaOH 2
It is connected to respectively with liquid-transfering needle group 1, suction pumps 3 and pressure control vessel 4, pressure control vessel 4 is connected to pulsometer 5；
Control module includes high-frequency electromagnetic valve V, the first solenoid valve V1, second solenoid valve V2, third solenoid valve V3 and ratio
Valve V4, high-frequency electromagnetic valve V are arranged in the fluid path between liquid-transfering needle group 1 and NaOH solution tank NaOH 2, and the first solenoid valve V1 is arranged in NaOH solution tank NaOH
In fluid path between 2 and pressure control vessel 4, second solenoid valve V2 is arranged in the fluid path between suction pumps 3 and NaOH solution tank NaOH 2,
Third solenoid valve V3 is arranged in the fluid path between suction pumps 3 and pressure control vessel 4, and proportioning valve V4 settings are controlled in pressure to be held
In gas circuit between device 4 and pulsometer 5.
Multiple reacting hole groups are provided on reaction plate, reacting hole group includes multiple linear distributing reacting holes, and adjacent
The distance between two reacting hole groups it is equal, reacting hole group is vertical with translation component moving direction, and liquid-transfering needle group 1 includes multiple
Linear distributing liquid-transfering needle, the position of multiple liquid-transfering needles and the position of reacting hole correspond, and liquid-transfering needle group 1 is fixed on translation
On the mobile terminal of component, and it is vertical with the translation moving direction of component.
Translation component is hydraulic stem, atmospheric pressure pole or linear motor, and 4 lower part of pressure control vessel is solution, top is air,
Pulsometer 5 is connected to the top of pressure control vessel 4, and the top of pressure control vessel 4 is connected with exhaust head, exhaust head and pressure
The 4th solenoid valve V5 is provided in gas circuit between control container 4.
A kind of liquid relief method based on above-mentioned contactless liquid-transfering device, liquid relief method include imbibition flow and hydrojet stream
Journey,
Imbibition flow includes after liquid relief pattern is selected and started, and liquid relief module is moved to designated position, the first solenoid valve V1
It closes, high-frequency electromagnetic valve V, second solenoid valve V2, third solenoid valve V3 and proportioning valve V4 are opened, i.e., first suck a certain amount of sky
Gas makes the pure water in pipeline be separated with the reagent that will be sucked, and avoids influencing experimental result；After sucking air, liquid relief module
It is moved to imbibition position, proceeds by imbibition action；
Hydrojet flow includes after sucking the liquid of set amount, and liquid relief module is moved to hydrojet position；When hydrojet, liquid relief module
Along setting path uniform motion, second solenoid valve V2 and third solenoid valve V3 are closed, and the first solenoid valve V1 and proportioning valve V4 are opened,
High-frequency electromagnetic valve V is switched according to the duration of valve opening of setting by certain frequency, interval time be the distance between reacting hole group with
The shifting speed ratio of component is translated, liquid uniformly sprays into each of reaction plate under the action of pulsometer 5 and high-frequency electromagnetic valve V in pipeline
In a reacting hole.
Technical scheme of the present invention is not limited to the limitation of above-mentioned specific embodiment, every to do according to the technique and scheme of the present invention
The technology deformation gone out, each falls within protection scope of the present invention.
Claims (5)
1. a kind of contactless liquid-transfering device, it is characterised in that：Including reaction plate, liquid relief module, component and control module are translated,
The translation component is fixed at the top of liquid relief position, and the reaction plate is placed on the liquid relief position, the liquid relief mould
The liquid relief end of block is fixedly connected with the mobile terminal of the translation component, and the control assembly is connect with the liquid relief module；
The liquid relief module includes liquid-transfering needle group, NaOH solution tank NaOH, suction pumps, pulsometer and pressure control vessel, the NaOH solution tank NaOH point
It is not connected to the liquid-transfering needle group, the suction pumps and the pressure control vessel, the pressure control vessel and the air pressure
Pump connection；
The control module includes high-frequency electromagnetic valve, the first solenoid valve, second solenoid valve, third solenoid valve and proportioning valve, described
High-frequency electromagnetic valve is arranged in the fluid path between the liquid-transfering needle group and the NaOH solution tank NaOH, and first solenoid valve is arranged described
In fluid path between NaOH solution tank NaOH and the pressure control vessel, the second solenoid valve is arranged in the suction pumps and the solution
In fluid path between tank, the third solenoid valve is arranged in the fluid path between the suction pumps and the pressure control vessel,
The proportioning valve is arranged in the gas circuit between the pressure control vessel and the pulsometer.
2. a kind of contactless liquid-transfering device according to claim 1, it is characterised in that：It is provided on the reaction plate more
A reacting hole group, the reacting hole group include multiple linear distributing reacting holes, and between two adjacent reacting hole groups
Apart from equal, the reacting hole group is vertical with the translation component moving direction；
The liquid-transfering needle group includes multiple linear distributing liquid-transfering needles, position and the reacting hole of multiple liquid-transfering needles
Position correspond, the liquid-transfering needle group be fixed on it is described translation component mobile terminal on, and with it is described translation component movement
Direction is vertical.
3. a kind of contactless liquid-transfering device according to claim 1, it is characterised in that：The translation component is hydraulic pressure
Bar, atmospheric pressure pole or linear motor.
4. a kind of contactless liquid-transfering device according to claim 1, it is characterised in that：The pressure control vessel lower part
Be air for solution, top, the pulsometer is connected to the top of the pressure control vessel, the pressure control vessel it is upper
Portion is connected with exhaust head, and the 4th solenoid valve is provided in the gas circuit between the exhaust head and the pressure control vessel.
5. a kind of liquid relief method based on above-mentioned contactless liquid-transfering device, it is characterised in that：The liquid relief method includes imbibition
Flow and hydrojet flow, specifically include following steps：
S1, the first solenoid valve are closed, and high-frequency electromagnetic valve, second solenoid valve, third solenoid valve and proportioning valve are opened；
S2, a certain amount of air of sucking；
S3, liquid-transfering needle group is moved to imbibition position, it will be in liquid inhalation solution tank and pressure control vessel；
S4, by reaction plate as liquid relief position, liquid-transfering needle group is moved to the top of reaction plate；
S5, translation component at the uniform velocity move, and second solenoid valve and third solenoid valve are closed, and the first solenoid valve and proportioning valve are opened；
S6, the open frequency for adjusting high-frequency electromagnetic valve, interval time are the shifting of the distance between reacting hole group and translation component
Speed ratio, liquid spray into reacting hole from liquid-transfering needle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810614967.7A CN108636469A (en) | 2018-06-14 | 2018-06-14 | A kind of contactless liquid-transfering device and liquid relief method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810614967.7A CN108636469A (en) | 2018-06-14 | 2018-06-14 | A kind of contactless liquid-transfering device and liquid relief method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108636469A true CN108636469A (en) | 2018-10-12 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810614967.7A Pending CN108636469A (en) | 2018-06-14 | 2018-06-14 | A kind of contactless liquid-transfering device and liquid relief method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109652288A (en) * | 2019-02-25 | 2019-04-19 | 成都瀚辰光翼科技有限责任公司 | A kind of automation Genotyping device and method |
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| WO2003065049A2 (en) * | 2002-01-25 | 2003-08-07 | Innovadyne Technologies, Inc. | Low volume, non-contact liquid dispensing method |
| US20030215957A1 (en) * | 1998-02-20 | 2003-11-20 | Tony Lemmo | Multi-channel dispensing system |
| CN1603009A (en) * | 2004-10-28 | 2005-04-06 | 北京博奥生物芯片有限责任公司 | Trace amount liquid jet system |
| CN2740589Y (en) * | 2004-06-11 | 2005-11-16 | 杭州华大基因研发中心 | Liquid transferring device and component of pressure variable cavity therewith |
| CN103998144A (en) * | 2011-10-28 | 2014-08-20 | 惠普发展公司，有限责任合伙企业 | Parallel addressing method |
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2018
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030215957A1 (en) * | 1998-02-20 | 2003-11-20 | Tony Lemmo | Multi-channel dispensing system |
| WO2003065049A2 (en) * | 2002-01-25 | 2003-08-07 | Innovadyne Technologies, Inc. | Low volume, non-contact liquid dispensing method |
| CN2740589Y (en) * | 2004-06-11 | 2005-11-16 | 杭州华大基因研发中心 | Liquid transferring device and component of pressure variable cavity therewith |
| CN1603009A (en) * | 2004-10-28 | 2005-04-06 | 北京博奥生物芯片有限责任公司 | Trace amount liquid jet system |
| CN103998144A (en) * | 2011-10-28 | 2014-08-20 | 惠普发展公司，有限责任合伙企业 | Parallel addressing method |
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| 刘绍力等: "《液压与气动》", 31 August 2017, 华中科技大学出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109652288A (en) * | 2019-02-25 | 2019-04-19 | 成都瀚辰光翼科技有限责任公司 | A kind of automation Genotyping device and method |
| CN109652288B (en) * | 2019-02-25 | 2022-06-28 | 成都瀚辰光翼科技有限责任公司 | Automatic genotyping equipment and method |
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Application publication date: 20181012 |
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