CN108624543A - One plant effectively controls eggplant Raul Salmonella and promotes the bacillus amyloliquefaciens of chili growth - Google Patents
One plant effectively controls eggplant Raul Salmonella and promotes the bacillus amyloliquefaciens of chili growth Download PDFInfo
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- CN108624543A CN108624543A CN201810771658.0A CN201810771658A CN108624543A CN 108624543 A CN108624543 A CN 108624543A CN 201810771658 A CN201810771658 A CN 201810771658A CN 108624543 A CN108624543 A CN 108624543A
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Environmental Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
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Abstract
The present invention provides one plant and can effectively control eggplant Raul Salmonella and promote the bacillus amyloliquefaciens (Bacillus amyloliquefaciens) of chili growth, belongs to microorganisms technical field.The bacterial strain is bacillus amyloliquefaciens CAS02, is isolated from tobacco rhizosphere, which is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on March 26th, 2018, and biological deposits number is CGMCC NO.15514.Bacillus amyloliquefaciens strain (Bacillus amyloliquefaciens) CAS02 of the present invention not only has growth-promoting functions to pepper plant, can also inhibit the growth of a variety of pathogens such as pepper ralstonia solanacearum bacterium, therefore have extraordinary application prospect.
Description
Technical field
The invention belongs to microorganisms technical fields, and in particular to one plant effectively controls eggplant Raul Salmonella and promotes chili growth
Bacillus amyloliquefaciens.
Background technology
Capsicum (Capsicum annuum L), belongs to Solanaceae, Capsicum, 1 year or limited herbaceos perennial, are us
The important vegetable crop of state and the raw material of industry.Bacterial wilt is caused by eggplant Raul Salmonella (Ralstonia solanacearum)
A kind of destructiveness soil-borne vascular bundle disease on the solanaceous crops such as capsicum, tomato, tobacco.The disease is distributed widely in the torrid zone, subtropical zone
And certain Temperate Region in China, it is green withered in world wide with the emergence of new host and the factors such as fast development of industrialized agriculture
The generation of disease is also more and more common and serious.Bacterial wilt has generation and harm in China overwhelming majority provinces and regions at present.According to kind
The difference of environmental condition and kind is planted, the bacterial wilt caused by eggplant Raul Salmonella has certain difference to the harm of plant,
Fall ill serious area can cause crop Severe Reduction even have no harvest, cause huge economic loss.Therefore, reinforce the anti-of bacterial wilt
It controls and has important practical significance.
Prevention for plant disease, generally use cultivate disease-resistant variety and the method using chemical agent.But it is disease-resistant
The selection and breeding of kind are mostly to be directed to some specific cause of disease microspecies, have certain limitation, and the Breeding Process of disease-resistant variety
Generally require years.It is a large amount of to be had been to be concerned by more and more people using the negative effect that chemical pesticide is brought, especially
The use of some high poison persistent pesticide is gradually limited by government policy.It is that biology is anti-using microbial control plant disease
One of important form controlled is a kind of environmentally friendly control method, and reduce the effective measures that use of chemical pesticide it
One.Many microorganisms are the natural enemies of phytopathogen, especially have inborn advantage in terms of preventing soil-borne disease.Gemma bar
Bacterium is that one of widest gram-positive bacterium is distributed in nature, is distributed widely in the environment such as water, soil.Because of gemma
The resistance of bacillus is strong, and many bacterial strains can promote plant growth, inhibit harm of the pathogen to plant, and as agricultural
A kind of biocontrol microorganisms being most widely used in production.However there has been no about can effectively prevent eggplant Raul Salmonella etc. so far
A variety of pathogens can effectively facilitate the bacillus amyloliquefaciens (Bacillus of chili growth again simultaneously
Amyloliquefaciens report).
Invention content
For the above-mentioned problems in the prior art, the purpose of the present invention is to provide one plant to have inhibition capsicum blueness withered
Bacillus amyloliquefaciens strain (Bacillus amyloliquefaciens) CAS02 of a variety of pathogens such as germ, peppery
The biological control of green pepper bacterial wilt promotes chili growth etc. and has extraordinary effect, is that Main Diseases in Chili Peppers bacterial wilt etc. is anti-
It controls and provides new microbial resources.
To achieve the above object, the present invention relates to following technical schemes：
The first aspect of the invention provides one plant of disease prevention growth-promoting Bacillus amyloliquefaciens strain (Bacillus
Amyloliquefaciens) CAS02, the bacterial strain have been preserved in Chinese microorganism strain preservation management committee on March 26th, 2018
Member can common micro-organisms center (address：City of BeiJing, China Chaoyang District North Star West Road 1 institute 3), biological deposits number are CGMCC
NO.15514。
The second aspect of the invention provides above-mentioned Bacillus amyloliquefaciens strain (Bacillus
Amyloliquefaciens) the cultural method of CAS02, including by Bacillus amyloliquefaciens strain (Bacillus
Amyloliquefaciens) CAS02 is placed in LB liquid medium and cultivates, and condition of culture is aerobic culture, such as shaking table at 28 DEG C
(28 DEG C, 150rpm) shaken cultivation.
Wherein, the LB culture medium prescriptions (g/L) are：Tryptone 10.0；Yeast extract 5.0；Sodium chloride 10.0；
pH7.0±0.2；
The third aspect of the invention, provides a kind of microbial bacterial agent, and the microbial bacterial agent includes solution starch gemma bar
Bacteria strain (Bacillus amyloliquefaciens) CAS02 or Bacillus amyloliquefaciens strain (Bacillus
Amyloliquefaciens) the culture of CAS02.The microbial bacterial agent, which has, inhibits pear tree fire blast (Erwinia
Amylovora 2029), bacterial soft rot of potato (Pectobacterium carotovorum), cotton angular leaf spot
(Xanthomonas campestris MSCT1), tobacco bacterial wilt (Ralstonia solanacearum), wildfire
(Pseudomonas syringae pv.tabaci), pepper anthracnose (Colletotrichum acutatum) and promotion
The effect of chili growth.
Preferably, the dosage form of microbial bacterial agent is that wettable powder, water dispersible granules, aqueous suspension agent or dispersible oil are outstanding
Floating agent；
Preferably, further include acceptable auxiliary material in Pesticide Science in microbial bacterial agent, it is acceptable in the Pesticide Science
One kind in dispersant, wetting agent, disintegrant, binder, antifoaming agent, antifreeze, thickener, filler and solvent of auxiliary material or
It is a variety of.The present invention is not particularly limited the source etc. of acceptable auxiliary material in the Pesticide Science, is generally using commercial product
It can.
The fourth aspect of the invention, provides a kind of microbial-bacterial fertilizer, and the microbial-bacterial fertilizer includes above-mentioned solution starch bud
Spore bacillus strain (Bacillus amyloliquefaciens) CAS02 or Bacillus amyloliquefaciens strain (Bacillus
Amyloliquefaciens) the culture of CAS02.The microbial-bacterial fertilizer, which has, inhibits pear tree fire blast (Erwinia
Amylovora 2029), bacterial soft rot of potato (Pectobacterium carotovorum), cotton angular leaf spot
(Xanthomonas campestris MSCT1), tobacco bacterial wilt (Ralstonia solanacearum), wildfire
(Pseudomonas syringae pv.tabaci), pepper anthracnose (Colletotrichum acutatum) and promotion
The effect of chili growth.
Preferably, microbial-bacterial fertilizer also contains organic matter, full potassium, full nitrogen, for providing nutrient.
The fifth aspect of the invention provides above-mentioned Bacillus amyloliquefaciens strain (Bacillus
Amyloliquefaciens) the application of CAS02, microbial bacterial agent and/or microbial-bacterial fertilizer in phytobacterial disease prevention.
Preferably, the phytobacterial disease includes but not limited to pear tree fire blast (Erwinia
Amylovora2029), bacterial soft rot of potato (Pectobacterium carotovorum), cotton angular leaf spot
(Xanthomonas campestris MSCT1), tobacco bacterial wilt (Ralstonia solanacearum), wildfire
(Pseudomonas syringae pv.tabaci) and pepper anthracnose (Colletotrichum acutatum).
The sixth aspect of the invention provides above-mentioned Bacillus amyloliquefaciens strain (Bacillus
Amyloliquefaciens) the application of CAS02, microbial bacterial agent and/or microbial-bacterial fertilizer in promoting chili growth.
Beneficial effects of the present invention：
The present invention is sieved from tobacco healthy tree rhizosphere soil for the first time by indoor flat plate face-off method and greenhouse pot culture control effect
Select a bacillus amyloliquefaciens bacterial strain (Bacillus amyloliquefaciens) CAS02, the bacterial strain diseases prevention, growth-promoting
Effect is all good, and its resistance is strong, which has both pathogen antagonistic activity, and antimicrobial spectrum is wide, for phytobacterial disease
Have especially for great in the prevention of pepper ralstonia solanacearum caused by eggplant Raul Salmonella (Ralstonia solanacearum)
Application and development potentiality；And it is proved through pot experiment, bacillus amyloliquefaciens (Bacillus amyloliquefaciens) CAS02
Also there is apparent growth-promoting effect to capsicum.Therefore, Bacillus amyloliquefaciens strain (Bacillus of the invention
Amyloliquefaciens) CAS02 to phytobacterial diseases such as prevention bacterial wilts and promotes chili growth to have important show
Sincere justice.
Description of the drawings
Fig. 1 is antagonism figure of the CAS02 bacterial strains to pathogen, wherein Figure 1A, pear tree fire blast bacterium (Erwinia
amylovora 2029)；Figure 1B, bacterial soft rot of potato germ (Pectobacterium carotovorum)；Fig. 1 C, cotton angle
Pinta bacterium (Xanthomonas campestris MSCT1)；Fig. 1 D, tobacco ralstonia solanacearum (Ralstonia
solanacearum)；Fig. 1 E, tobacco smoke pollution (Pseudomonas syringae pv.tabaci)；Fig. 1 F, capsicum anthrax
Germ (Colletotrichum acutatum).
Fig. 2 is the systematic evolution tree based on 16S rDNA structures.
Fig. 3 is growth-promoting functions design sketch of the bacterial strain CAS02 to capsicum, wherein A is bacterial strain CAS02 processing groups；B is control
Group.
Specific implementation mode
It is noted that following detailed description is all illustrative, it is intended to provide further instruction to the application.Unless another
It indicates, all technical and scientific terms used herein has usual with the application person of an ordinary skill in the technical field
The identical meanings of understanding.
It should be noted that term used herein above is merely to describe specific implementation mode, and be not intended to restricted root
According to the illustrative embodiments of the application.As used herein, unless the context clearly indicates otherwise, otherwise singulative
It is also intended to include plural form, additionally, it should be understood that, when in the present specification using term "comprising" and/or " packet
Include " when, indicate existing characteristics, step, operation, device, component and/or combination thereof.
In conjunction with specific example, the present invention is further illustrated, and following instance is not right merely to the explanation present invention
Its content is defined.If the experiment actual conditions being not specified in embodiment, usually according to normal condition, or according to reagent public affairs
The recommended condition of department；Reagent as used in the following examples, consumptive material etc., are commercially available unless otherwise specified.
As previously mentioned, so far there has been no about can effectively control a variety of pathogens such as eggplant Raul Salmonella simultaneously but also
Effectively facilitate the report of the bacillus of chili growth.
In view of this, in the specific embodiment of the present invention, one plant of disease prevention growth-promoting bacillus amyloliquefaciens bacterium is provided
Strain (Bacillus amyloliquefaciens) CAS02, the bacterial strain are preserved in China Microbiological bacterium on March 26th, 2018
Kind preservation administration committee's common micro-organisms center (address：City of BeiJing, China Chaoyang District North Star West Road 1 institute 3), biology
Preserving number is CGMCC NO.15514.
In the present invention, by measuring bacterial strain 16S rDNA gene orders (see SEQ ID NO.1), the bacterial strain is finally determined
Belong to Bacillus amyloliquefaciens strain (Bacillus amyloliquefaciens) CAS02.
In the still another embodiment of the present invention, above-mentioned Bacillus amyloliquefaciens strain (Bacillus is provided
Amyloliquefaciens) the cultural method of CAS02, including by Bacillus amyloliquefaciens strain (Bacillus
Amyloliquefaciens) CAS02 is placed in LB liquid medium and cultivates, and condition of culture is aerobic culture, such as shaking table at 28 DEG C
(28 DEG C, 150rpm) shaken cultivation.
Wherein, the LB culture medium prescriptions (g/L) are：Tryptone 10.0；Yeast extract 5.0；Sodium chloride 10.0；
pH7.0±0.2；
In the still another embodiment of the present invention, a kind of microbial bacterial agent is provided, the microbial bacterial agent includes Xie Dian
Afnyloliquefaciens bacterial strain (Bacillus amyloliquefaciens) CAS02 or Bacillus amyloliquefaciens strain (Bacillus
Amyloliquefaciens) the culture of CAS02.The microbial bacterial agent, which has, inhibits pear tree fire blast (Erwinia
Amylovora 2029), bacterial soft rot of potato (Pectobacterium carotovorum), cotton angular leaf spot
(Xanthomonas campestris MSCT1), tobacco bacterial wilt (Ralstonia solanacearum), wildfire
(Pseudomonas syringae pv.tabaci), pepper anthracnose (Colletotrichum acutatum) and promotion
The effect of chili growth.
In the still another embodiment of the present invention, the dosage form of microbial bacterial agent is wettable powder, water dispersible granules, water
Suspending agent or dispersible oil-suspending agent；
Further include acceptable auxiliary material in Pesticide Science, institute in microbial bacterial agent in the still another embodiment of the present invention
Acceptable auxiliary material in Pesticide Science is stated to be selected from dispersant, wetting agent, disintegrant, binder, antifoaming agent, antifreeze, thickener, fill out
It is one or more in material and solvent.The present invention is not particularly limited the source etc. of acceptable auxiliary material in the Pesticide Science,
Generally use commercial product.
Wherein, the dispersant is anionic dispersing agent and/or non-ionic dispersing agent, can be selected from lignin sulfonic acid
Sodium, naphthalenesulfonic acid-formaldehyde condensate, sodium methylene bis-naphthalene sulfonate, formaldehyde condensation products sulfate, polycarboxylate, alkyl phenol polyoxy second
It is one or more in alkenyl phosphate and polyoxyethylene carboxylate；
The wetting agent can be selected from lauryl sodium sulfate, neopelex, spaonin powder, spaonin powder, tea seed cake
It is one or more in powder and Nekal BX；
The disintegrant can be selected from one kind or more in bentonite, ammonium sulfate, aluminium chloride, urea, magnesium chloride and glucose
Kind；
The binder can be selected from starch, diatomite, cyclodextrin, rosin, carboxymethyl cellulose, carboxyethyl cellulose and carboxylic
It is one or more in methylcellulose salt；
The antifoaming agent can be selected from C8~C20 aliphatic alcohols compound, C10~C20 saturated fat acids compound, epoxy
It is one or more in soybean oil, ethyl alcohol, silicone compound and organic silicone oil；
The antifreeze can be selected from sorbierite, ethylene glycol, polyethylene glycol, propylene glycol, glycerine, urea and sodium chloride
It is one or more；
The thickener can be selected from one or more in gelatin, xanthans, polyethylene glycol and polyvinyl alcohol；
The filler can be selected from one kind or more in precipitated calcium carbonate, diatomite, bentonite, attapulgite and white carbon
Kind；
The solvent can be selected from water (preferably deionized water) or methyl oleate；
The still another embodiment of the present invention, provides a kind of microbial-bacterial fertilizer, the microbial-bacterial fertilizer includes above-mentioned solution
Bacillus amyloliquefaciens bacterial strain (Bacillus amyloliquefaciens) CAS02 or Bacillus amyloliquefaciens strain
The culture of (Bacillus amyloliquefaciens) CAS02.The microbial-bacterial fertilizer, which has, inhibits pear tree fire blast
(Erwinia amylovora 2029), bacterial soft rot of potato (Pectobacterium carotovorum), cotton angular leaf spot
(Xanthomonas campestris MSCT1), tobacco bacterial wilt (Ralstonia solanacearum), wildfire
(Pseudomonas syringae pv.tabaci), pepper anthracnose (Colletotrichum acutatum) and promotion
The effect of chili growth.
In the still another embodiment of the present invention, the microbial-bacterial fertilizer also contains organic matter, full potassium, full nitrogen, is used for
Nutrient is provided.
In the still another embodiment of the present invention, above-mentioned Bacillus amyloliquefaciens strain (Bacillus is provided
Amyloliquefaciens) the application of CAS02, microbial bacterial agent and/or microbial-bacterial fertilizer in phytobacterial disease prevention.
In the still another embodiment of the present invention, the phytobacterial disease includes but not limited to pear tree fire blast
(Erwinia amylovora 2029), bacterial soft rot of potato (Pectobacterium carotovorum), cotton angular leaf spot
(Xanthomonas campestris MSCT1), tobacco bacterial wilt (Ralstonia solanacearum), wildfire
(Pseudomonas syringae pv.tabaci) and pepper anthracnose (Colletotrichum acutatum).
In the still another embodiment of the present invention, above-mentioned Bacillus amyloliquefaciens strain (Bacillus is provided
Amyloliquefaciens) the application of CAS02, microbial bacterial agent and/or microbial-bacterial fertilizer in promoting chili growth.
Explanation is further explained to the present invention by the following examples, but is not construed as limiting the invention.It should be understood that
These examples are only for illustrating the present invention and are not intended to limit the scope of the present invention.It is to indicate actual conditions in the following example
Test method, carry out usually according to normal condition.
Embodiment 1：Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) CAS02 bacteriostasis measures
The bacterial strain (CAS02) is isolated from Qingdao tobacco rhizosphere soil.Using opposite culture method (Sijam etc., 2005)
Measure bacteriostasis of the bacterial strain to pathogen.10 μ L bacteria suspensions (OD420=0.3) are drawn, the NBY for being inoculated in a diameter of 9cm is solid
Culture dish is put into 28 DEG C of biochemical cultivation cases after bacterium solution fully absorbs and is inverted culture 72h by body culture medium central.Then divide
OD is not sprayed to NBY culture mediums420=0.3 cause of disease bacteria suspension.Culture dish is transferred to 28 DEG C of constant incubators again, is inverted
After culture for 24 hours, the bacteriostasis of bacterial strain is measured, as a result shows bacterial strain CAS02 to pear tree fire blast (Erwinia
Amylovora 2029), bacterial soft rot of potato (Pectobacterium carotovorum), cotton angular leaf spot
(Xanthomonas campestris MSCT1), tobacco bacterial wilt (Ralstonia solanacearum), wildfire
Bacterium (Pseudomonas syringae pv.tabaci) and pepper anthracnose (Colletotrichum acutatum) all have
The antagonistic effect (Fig. 1) of highly significant.
Embodiment 2：The acquisition of bacterial strain 16S rDNA sequences
Using bacillus amyloliquefaciens (Bacillus amyloliquefaciens) CAS02 genomic DNAs template, utilize
Bacterial 16 S rDNA universal primers 27F (5 '-AGAGTTTGATCMTGGCTCAG-3 ') and 1492R (5 '-
TACGGYTACCTTGTTACGACTT-3 ') amplification 16S rDNA sequences.Amplification system and program：Include in the PCR system of 25 μ L
5 × PCR buffer solutions, 5.0 μ L, DNA profiling (100ng μ L-1) 1.0 μ L, dNTPs (10mM) 0.75 μ L, forward primer (10 μM)
0.75 μ L, 0.75 2.5 μ L, Taq archaeal dna polymerase (5U μ L-1Promega) 0.25 of μ L, MgCl2 (25mM) of reverse primer (10 μM)
μ L, rest part ddH2O polishings.PCR amplification program is 94 DEG C of pre-degeneration 3min；94 DEG C of denaturation 30s, anneal 30s, 72 DEG C of extensions
1min30s, 30 cycles.Finally, 72 DEG C of extension 10min, 4 DEG C of preservations.After reaction, agarose is carried out to PCR product to coagulate
Gel electrophoresis is analyzed.The segment of acquisition is subjected to sequencing analysis.Utilize MEGA software buildings systematic evolution tree (Fig. 2).
The 16S rDNA sequences of CAS02 and bacillus amyloliquefaciens (Bacillus amyloliquefaciens) FZB42
Concordance rate is 99.8%.In the systematic evolution tree according to 16S rDNA sequence constructs, CAS02 gathers with bacillus amyloliquefaciens is
Cluster shows that CAS02 belongs to bacillus amyloliquefaciens.
Embodiment 3：The growth-promoting experiment of the greenhouses bacterial strain CAS02
The consistent pepper seedling of growing way is transplanted in the matrix to sterilizing, it, will when growth of seedling to two panels true leaf
CAS02 bacterium are outstanding slowly to pour into capsicum rhizosphere, every plant of 20ml.Control group pours isometric Huo Gelan nutrient solutions.It is measured after 28d
Growth-promoting effects of the CAS02 to capsicum.Logical control is compared, and the growth of pepper plant can be remarkably promoted with CAS02 processing, plant
Fresh weight and aerial part plant height increase separately 81.25% and 34.23%, and chlorophyll a and content of chlorophyll b difference are compared with control
Increase by 29.09% and 17.63% (table 1, Fig. 2).
Growth-promoting functions of the 1 bacterial strain CAS02 of table to capsicum
Note：Data are the average value repeated three times.Different letters indicate that in the same period different disposal is in p<0.01 it is aobvious
It writes under level, otherness is significantly (LSD test)
Embodiment 4：Biocontrol effects of the bacterial strain CAS02 to pepper ralstonia solanacearum
After capsicum transplanting, CAS02 bacteria suspensions are slowly poured into capsicum rhizosphere, every plant of 20ml, control group pours isometric
Huo Gelan nutrient solutions.Eggplant Raul Salmonella is inoculated with after 7d around capsicum rhizosphere, incidence is counted after every plant of 20ml, 28d.According to
The bacterial wilt disease scale standard that Kempe (1983) is proposed, 5 grades are divided into according to 0,1,2,3,4.0 grade：Without green withered disease
Shape；1 grade：There is wilting symptom in 1~25% blade；2 grades：There is wilting symptom in 26~50% blade；3 grades：51~75%
Blade there is wilting symptom；4 grades：There is wilting symptom in 76~100% blade.Disease index and biocontrol effect are according to as follows
Formula calculates.Disease index=100 × ∑ (disease plant number × disease series)/(total strain number × highest disease series)；Opposite prevention
Effect (%)=(control disease index-processing disease index)/control disease index × 100.
Compared with the control, the plant incidence lower than control group 33.34% of CAS02 processing, disease index low 47.5, preventive effect
Reach 67.86% (table 2).
Prophylaxis effects of the 2 bacterial strain CAS02 of table to capsicum
Note：Data are the average value repeated three times.Different letters indicate that in the same period different disposal is in p<0.01 it is aobvious
It writes under level, otherness is significantly (LSD test).
It should be noted that above example is only used to illustrate the technical scheme of the present invention rather than is limited.Although ginseng
It is described the invention in detail according to given example, but those skilled in the art can be as needed to this hair
Bright technical solution is modified or replaced equivalently, without departing from the spirit of the technical scheme of the invention and range.
SEQUENCE LISTING
<110>Tobacco Institute, Chinese Academy of Agricultural Science
<120>One plant effectively controls eggplant Raul Salmonella and promotes the bacillus amyloliquefaciens of chili growth
<130>
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 1398
<212> DNA
<213>Bacillus amyloliquefaciens Bacillus amyloliquefaciens 16S rDNA
<400> 1
gtcgagcgga cagatgggag cttgctccct gatgttagcg gcggacgggt gagtaacacg 60
tgggtaacct gcctgtaaga ctgggataac tccgggaaac cggggctaat accggatggt 120
tgtctgaacc gcatggttca gacataaaag gtggcttcgg ctaccactta cagatggacc 180
cgcggcgcat tagctagttg gtgaggtaac ggctcaccaa ggcgacgatg cgtagccgac 240
ctgagagggt gatcggccac actgggactg agacacggcc cagactccta cgggaggcag 300
cagtagggaa tcttccgcaa tggacgaaag tctgacggag caacgccgcg tgagtgatga 360
aggttttcgg atcgtaaagc tctgttgtta gggaagaaca agtgccgttc aaatagggcg 420
gcaccttgac ggtacctaac cagaaagcca cggctaacta cgtgccagca gccgcggtaa 480
tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agggctcgca ggcggtttct 540
taagtctgat gtgaaagccc ccggctcaac cggggagggt cattggaaac tggggaactt 600
gagtgcagaa gaggagagtg gaattccacg tgtagccggt gaaatgcgta gagatgtgga 660
ggaacaccag tggcgaaggc gactctctgg tctgtaactg acgctgagga gcgaaagcgt 720
ggggagcgaa caggattaga taccctggta gtccacgccg taaacgatga gtgctaagtg 780
ttagggggtt tccgcccctt tagtgctgca gctaacgcat taagcactcc gcctggggag 840
tacggtcgca agactgaaac tcaaaggaat tgacgggggc ccgcacaagc ggtggagcat 900
gtggtttaat tcgaagcaac gcgaagaacc ttaccaggtc ttgacatcct ctgacaatcc 960
tagagatagg acgtcccctt cgggggcaga gtgacaggtg gtgcatggtt gtcgtcagct 1020
cgtgtcgtga gatgttgggt taagtcccgc aacgagcgca acccttgatc ttagttgcca 1080
gcattcagtt gggcactcta aggtgactgc cggtgacaaa ccggaggaag gtggggatga 1140
cgtcaaatca tcatgcccct tatgacctgg gctacacacg tgctacaatg gacagaacaa 1200
agggcagcga aaccgcgagg ttaagccaat cccacaaatc tgttctcagt tcggatcgca 1260
gtctgcaact cgactgcgtg aagctggaat cgctagtaat cgcggatcag catgccgcgg 1320
tgaatacgtt cccgggcctt gtacacaccg cccgtcacac cacgagagtt tgtaacaccc 1380
gaagtcggtg aggtaacc 1398
Claims (10)
1. one plant of disease prevention growth-promoting Bacillus amyloliquefaciens strain (Bacillus amyloliquefaciens) CAS02, the bacterial strain
China Committee for Culture Collection of Microorganisms's common micro-organisms center, biological deposits are preserved on March 26th, 2018
Number be CGMCC NO.15514.
The culture of Bacillus amyloliquefaciens strain described in claim 1 2. (Bacillus amyloliquefaciens) CAS02
Method, which is characterized in that including Bacillus amyloliquefaciens strain (Bacillus amyloliquefaciens) CAS02 to be placed in
It is cultivated in LB liquid medium.
3. cultural method as claimed in claim 2, which is characterized in that condition of culture is aerobic culture at 28 DEG C, it is preferred that is used
Shaking table is at 28 DEG C, shaken cultivation under the conditions of 150rpm.
4. a kind of microbial bacterial agent, which is characterized in that the microbial bacterial agent includes Bacillus amyloliquefaciens strain (Bacillus
Amyloliquefaciens) CAS02 or Bacillus amyloliquefaciens strain (Bacillus amyloliquefaciens) CAS02
Culture.
5. microbial bacterial agent as claimed in claim 4, which is characterized in that the dosage form of microbial bacterial agent is wettable powder, water
Dispersible granule, aqueous suspension agent or dispersible oil-suspending agent.
6. microbial bacterial agent as claimed in claim 4, which is characterized in that further include being subjected in Pesticide Science in microbial bacterial agent
Auxiliary material, in the Pesticide Science acceptable auxiliary material be selected from dispersant, wetting agent, disintegrant, binder, antifoaming agent, antifreeze,
It is one or more in thickener, filler and solvent.
7. a kind of microbial-bacterial fertilizer, which is characterized in that the microbial-bacterial fertilizer includes above-mentioned Bacillus amyloliquefaciens strain
(Bacillus amyloliquefaciens) CAS02 or Bacillus amyloliquefaciens strain (Bacillus
Amyloliquefaciens) the culture of CAS02.
8. microbial-bacterial fertilizer as claimed in claim 7, which is characterized in that microbial-bacterial fertilizer also contains organic matter, full potassium, complete
Nitrogen.
9. Bacillus amyloliquefaciens strain described in claim 1 (Bacillus amyloliquefaciens) CAS02, right are wanted
Ask any one of 4-6 microbial bacterial agents and/or the microbial-bacterial fertilizer of claim 7 or 8 in phytobacterial disease prevention
Application.
Preferably, the phytobacterial disease includes pear tree fire blast (Erwinia amylovora 2029), potato rot positive
Sick (Pectobacterium carotovorum), cotton angular leaf spot (Xanthomonas campestris MSCT1), tobacco
Bacterial wilt (Ralstonia solanacearum), wildfire (Pseudomonas syringae pv.tabaci) and peppery
Green pepper anthracnose (Colletotrichum acutatum).
Bacillus amyloliquefaciens strain described in claim 1 10. (Bacillus amyloliquefaciens) CAS02, right
It is required that any one of 4-6 microbial bacterial agents and/or the microbial-bacterial fertilizer of claim 7 or 8 are in promoting chili growth
Using.
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| CN110373360A (en) * | 2019-08-07 | 2019-10-25 | 金华市农业科学研究院 | One bacillus amyloliquefaciens bacterial strain, microbial inoculum and preparation method thereof and the application in prevention and treatment ginger bacterial wilt |
| CN110612979A (en) * | 2019-10-12 | 2019-12-27 | 扬州大学 | Disease-resistant growth-promoting composite bacillus water dispersible granule as well as preparation method and application thereof |
| CN110923178A (en) * | 2019-09-25 | 2020-03-27 | 中国农业科学院农业资源与农业区划研究所 | Plant immunity inducing antibacterial agent and application thereof |
| CN112358366A (en) * | 2020-11-27 | 2021-02-12 | 新疆农业科学院微生物应用研究所（中国新疆-亚美尼亚生物工程研究开发中心） | Compound bacterial fertilizer for preventing and treating pear fire blight and preparation method and application thereof |
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| CN113637612A (en) * | 2021-09-03 | 2021-11-12 | 山东农业大学 | Salt-tolerant strain BY2G20 and application thereof |
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| CN110205273A (en) * | 2019-06-11 | 2019-09-06 | 山东碧蓝生物科技有限公司 | A kind of bacillus amyloliquefaciens and its application with growth promotion and resistant effect |
| CN110205273B (en) * | 2019-06-11 | 2021-02-09 | 山东碧蓝生物科技有限公司 | Bacillus amyloliquefaciens with growth promoting and disease resisting effects and application thereof |
| CN110373360A (en) * | 2019-08-07 | 2019-10-25 | 金华市农业科学研究院 | One bacillus amyloliquefaciens bacterial strain, microbial inoculum and preparation method thereof and the application in prevention and treatment ginger bacterial wilt |
| CN110923178A (en) * | 2019-09-25 | 2020-03-27 | 中国农业科学院农业资源与农业区划研究所 | Plant immunity inducing antibacterial agent and application thereof |
| CN110923178B (en) * | 2019-09-25 | 2021-06-18 | 中国农业科学院农业资源与农业区划研究所 | Plant immunity inducing antibacterial agent and application thereof |
| CN110612979A (en) * | 2019-10-12 | 2019-12-27 | 扬州大学 | Disease-resistant growth-promoting composite bacillus water dispersible granule as well as preparation method and application thereof |
| CN112358366A (en) * | 2020-11-27 | 2021-02-12 | 新疆农业科学院微生物应用研究所（中国新疆-亚美尼亚生物工程研究开发中心） | Compound bacterial fertilizer for preventing and treating pear fire blight and preparation method and application thereof |
| CN112553122A (en) * | 2020-12-25 | 2021-03-26 | 青岛农业大学 | Bacillus amyloliquefaciens, microbial inoculum and preparation method and application thereof |
| CN113637612A (en) * | 2021-09-03 | 2021-11-12 | 山东农业大学 | Salt-tolerant strain BY2G20 and application thereof |
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