CN108610413A - A kind of safe and comfortable collagen peptide to hepatic injury with repair - Google Patents
A kind of safe and comfortable collagen peptide to hepatic injury with repair Download PDFInfo
- Publication number
- CN108610413A CN108610413A CN201810369285.4A CN201810369285A CN108610413A CN 108610413 A CN108610413 A CN 108610413A CN 201810369285 A CN201810369285 A CN 201810369285A CN 108610413 A CN108610413 A CN 108610413A
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- Prior art keywords
- comfortable
- safe
- collagen peptide
- enzymolysis
- ultrafiltration
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- 102000008186 Collagen Human genes 0.000 title claims abstract description 22
- 108010035532 Collagen Proteins 0.000 title claims abstract description 22
- 229920001436 collagen Polymers 0.000 title claims abstract description 22
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 21
- 231100000753 hepatic injury Toxicity 0.000 title claims abstract description 13
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 21
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 241001417045 Lophius litulon Species 0.000 claims abstract description 12
- 239000007788 liquid Substances 0.000 claims abstract description 10
- 208000008338 non-alcoholic fatty liver disease Diseases 0.000 claims abstract description 9
- 238000011282 treatment Methods 0.000 claims abstract description 9
- 108091005804 Peptidases Proteins 0.000 claims abstract description 8
- 239000004365 Protease Substances 0.000 claims abstract description 8
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 8
- 239000003513 alkali Substances 0.000 claims abstract description 8
- 239000012153 distilled water Substances 0.000 claims abstract description 8
- 239000000843 powder Substances 0.000 claims abstract description 8
- 102000004190 Enzymes Human genes 0.000 claims abstract description 7
- 108090000790 Enzymes Proteins 0.000 claims abstract description 7
- 239000002994 raw material Substances 0.000 claims abstract description 5
- 235000011054 acetic acid Nutrition 0.000 claims abstract description 4
- 150000001243 acetic acids Chemical class 0.000 claims abstract description 4
- 230000009849 deactivation Effects 0.000 claims abstract description 4
- 238000011033 desalting Methods 0.000 claims abstract description 4
- 239000012528 membrane Substances 0.000 claims abstract description 4
- 238000001728 nano-filtration Methods 0.000 claims abstract description 4
- 239000006228 supernatant Substances 0.000 claims abstract description 4
- 238000002360 preparation method Methods 0.000 claims description 11
- TUNFSRHWOTWDNC-UHFFFAOYSA-N tetradecanoic acid Chemical compound CCCCCCCCCCCCCC(O)=O TUNFSRHWOTWDNC-UHFFFAOYSA-N 0.000 claims description 6
- 235000013305 food Nutrition 0.000 claims description 4
- IZXIZTKNFFYFOF-UHFFFAOYSA-N 2-Oxazolidone Chemical class O=C1NCCO1 IZXIZTKNFFYFOF-UHFFFAOYSA-N 0.000 claims description 3
- 239000004698 Polyethylene Substances 0.000 claims description 3
- KJADKKWYZYXHBB-XBWDGYHZSA-N Topiramic acid Chemical compound C1O[C@@]2(COS(N)(=O)=O)OC(C)(C)O[C@H]2[C@@H]2OC(C)(C)O[C@@H]21 KJADKKWYZYXHBB-XBWDGYHZSA-N 0.000 claims description 3
- 239000000470 constituent Substances 0.000 claims description 3
- 239000003814 drug Substances 0.000 claims description 3
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 3
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 3
- 238000004108 freeze drying Methods 0.000 claims description 3
- 239000004615 ingredient Substances 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- -1 polyethylene Polymers 0.000 claims description 3
- 229920000573 polyethylene Polymers 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 229960004394 topiramate Drugs 0.000 claims description 3
- 229940079593 drug Drugs 0.000 claims description 2
- 235000013402 health food Nutrition 0.000 claims description 2
- 239000000825 pharmaceutical preparation Substances 0.000 claims description 2
- 238000000034 method Methods 0.000 description 4
- 230000000295 complement effect Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 239000005541 ACE inhibitor Substances 0.000 description 1
- 101710129690 Angiotensin-converting enzyme inhibitor Proteins 0.000 description 1
- 101710086378 Bradykinin-potentiating and C-type natriuretic peptides Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 229940044094 angiotensin-converting-enzyme inhibitor Drugs 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 210000005229 liver cell Anatomy 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/01—Hydrolysed proteins; Derivatives thereof
- A61K38/012—Hydrolysed proteins; Derivatives thereof from animals
- A61K38/014—Hydrolysed proteins; Derivatives thereof from animals from connective tissue peptides, e.g. gelatin, collagen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Gastroenterology & Hepatology (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Epidemiology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Toxicology (AREA)
- Biophysics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
A kind of safe and comfortable collagen peptide to hepatic injury with repair, it is preprocessed, the alkali protease enzymolysis and ultrafiltration obtains using anglerfish skin as raw material, it is described it is pretreated the specific steps are：First fish-skin is cleaned up, 10-30min are impregnated in 2-8 DEG C of distilled water, then 20-are impregnated for 24 hours with 0.08-0.12mol/L sodium hydroxide solutions of 6-12 times of volumes, then it is washed to neutrality with clear water, it drains away the water, 9-15h are swollen with 0.08-0.12mol/L glacial acetic acids, crushing obtains anglerfish skin powder；The enzymolysis the specific steps are：Distilled water is added in anglerfish skin powder and alkali protease digests, enzyme deactivation after enzymolysis, centrifuging and taking supernatant；The ultrafiltration the specific steps are：It uses 8-10KDa, 5-8KDa, 3-5KDa, 1-3KDa, 1KDa ultrafiltration membranes below to carry out ultrafiltration to enzymolysis liquid successively, takes molecular weight 1KDa with lower part, nanofiltration is freeze-dried after desalting；The safe and comfortable collagen peptide is used for the treatment of non-alcoholic fatty liver disease.
Description
Technical field
The present invention relates to a kind of safe and comfortable collagen peptides and purposes to hepatic injury with repair, belong to raw
Active substances extractive technique field.
Background technology
Non-alcoholic fatty liver disease（Nonalcoholic fatty liver disease, NAFLD）It is with liver cell fat
The pathological syndrome that fat is denaturalized and fat accumulation is main feature, the method treated at present mainly take weight reducing treatments, lipid-loweringing to control
The expectant treatments such as treatment and angiotensin converting enzyme inhibitor, but expectant treatment still lacks effect method, less effective at present.Closely
The researcher of Nian Lai, China have made largely to extracting biological active matter confrontation hepatic injury progress protective effect from marine animal
Research.
A large amount of safe and comfortable skin is abandoned in safe and comfortable product processing Zhong Lost, is extraction collagen egg containing abundant collagen in fish-skin
The good raw material of white peptide.
Invention content
It is an object of the invention to overcome the shortcomings of the prior art, and a kind of purity height is provided, to non-alcoholic fat
Fat liver model has apparent repair and property is stablized, and preparation method step is simple, recovery rate is high to non-alcoholic fatty
Liver model has the safe and comfortable collagen peptide significantly repaired and purposes.
The purpose of the present invention is by following technical solution to complete, a kind of Ankang to hepatic injury with repair
Collagen peptide, it is using anglerfish skin as raw material, and simultaneously ultrafiltration obtains preprocessed, alkali protease enzymolysis, the pre- place
Reason the specific steps are：First fish-skin is cleaned up, 10-30min are impregnated in 2-8 DEG C of distilled water, then with 6-12 times
0.08-0.12mol/L sodium hydroxide solutions of volume impregnate 20-for 24 hours, are then washed to neutrality, are drained away the water with clear water, use
0.08-0.12mol/L glacial acetic acids are swollen 9-15h, and crushing obtains anglerfish skin powder；
The enzymolysis the specific steps are：Distilled water and alkali protease enzymolysis are added in anglerfish skin powder, enzymolysis terminates
Enzyme deactivation afterwards, centrifuging and taking supernatant.
The ultrafiltration the specific steps are：Use successively 8-10KDa, 5-8KDa, 3-5KDa, 1-3KDa, 1KDa with
Under ultrafiltration membrane to enzymolysis liquid carry out ultrafiltration, take molecular weight 1KDa with lower part, nanofiltration is freeze-dried after desalting.
As preferred：0.0002-0.0005mol/LN- acetyl-D-Gln is added in the sodium hydroxide solution
With 0.0001-0.0002mol/LN- acetyl-L-glutamines；
The enzymatic hydrolysis condition is solid-liquid ratio 1:1.5-4,45-55 DEG C of temperature, pH8.5-9.5,8-12h of time, enzyme concentration
1500—2500U/g；
15-25wt% polyethylene Topiramate Los oxazolidinones and 0.5-0.9% tetradecanoic acid are added when the described freeze-drying.
A kind of purposes to safe and comfortable collagen peptide of the hepatic injury with repair, the safe and comfortable collagen
Peptide is used for the treatment of non-alcoholic fatty liver disease.
As preferred：The purposes is using safe and comfortable collagen peptide as active constituent, in addition drug, food or health care
What acceptable auxiliary material or complementary ingredient were prepared on food includes at least liquid preparation, solid pharmaceutical preparation or semisolid preparation
Preparation.
The present invention has purity height, has apparent repair to nonalcoholic fatty liver model and property is stablized, system
The features such as Preparation Method step is simple, recovery rate is high.
Specific implementation mode
Below in conjunction with specific embodiment, the present invention will be described in detail：Of the present invention a kind of having hepatic injury
The safe and comfortable collagen peptide of repair, it is preprocessed, alkali protease enzymolysis and ultrafiltration using anglerfish skin as raw material
Obtain, it is described it is pretreated the specific steps are：First fish-skin is cleaned up, 10-30min are impregnated in 2-8 DEG C of distilled water,
Then it impregnates 20-for 24 hours with 0.08-0.12mol/L sodium hydroxide solutions of 6-12 times of volumes, is then washed into clear water
Property, it drains away the water, 9-15h is swollen with 0.08-0.12mol/L glacial acetic acids, crushing obtains anglerfish skin powder；
The enzymolysis the specific steps are：Distilled water and alkali protease enzymolysis are added in anglerfish skin powder, enzymolysis terminates
Enzyme deactivation afterwards, centrifuging and taking supernatant.
The ultrafiltration the specific steps are：Use successively 8-10KDa, 5-8KDa, 3-5KDa, 1-3KDa, 1KDa with
Under ultrafiltration membrane to enzymolysis liquid carry out ultrafiltration, take molecular weight 1KDa with lower part, nanofiltration is freeze-dried after desalting.
As preferred embodiment, 0.0002-0.0005mol/LN- is added in sodium hydroxide solution of the present invention
Acetyl-D-Gln and 0.0001-0.0002mol/LN- acetyl-L-glutamines；
The enzymatic hydrolysis condition is solid-liquid ratio 1:1.5-4,45-55 DEG C of temperature, pH8.5-9.5,8-12h of time, enzyme concentration
1500—2500U/g；
15-25wt% polyethylene Topiramate Los oxazolidinones and 0.5-0.9% tetradecanoic acid are added when the described freeze-drying.
A kind of purposes to safe and comfortable collagen peptide of the hepatic injury with repair, the safe and comfortable collagen
Peptide is used for the treatment of non-alcoholic fatty liver disease；The treatment is using safe and comfortable collagen peptide as active constituent, in addition medicine
What acceptable auxiliary material or complementary ingredient were prepared on product, food or health food includes at least liquid preparation, solid system
The preparation of agent or semisolid preparation.
Other embodiments of the invention can pass through numerical value and routine techniques feature on the basis of embodiment disclosed above
Simple replacement, numerous embodiment can be obtained, and those skilled in the art is on the basis of understanding the content of present invention, energy
Enough it is advantageously carried out the present invention.
Claims (4)
1. a kind of safe and comfortable collagen peptide to hepatic injury with repair, it is using anglerfish skin as raw material, through locating in advance
Reason, alkali protease enzymolysis and ultrafiltration obtain, it is characterised in that：
It is described it is pretreated the specific steps are：First fish-skin is cleaned up, 10-30min are impregnated in 2-8 DEG C of distilled water, are connect
It and impregnates 20-for 24 hours with 0.08-0.12mol/L sodium hydroxide solutions of 6-12 times of volumes, then washed to neutrality with clear water,
It drains away the water, is swollen 9-15h with 0.08-0.12mol/L glacial acetic acids, crushing obtains anglerfish skin powder；
The enzymolysis the specific steps are：Distilled water and alkali protease enzymolysis are added in anglerfish skin powder, enzymolysis terminates
Enzyme deactivation afterwards, centrifuging and taking supernatant；
The ultrafiltration the specific steps are：Use 8-10KDa, 5-8KDa, 3-5KDa, 1-3KDa, 1KDa below successively
Ultrafiltration membrane carries out ultrafiltration to enzymolysis liquid, takes molecular weight 1KDa with lower part, nanofiltration is freeze-dried after desalting.
2. the safe and comfortable collagen peptide to hepatic injury with repair according to claim 1, it is characterised in that：
0.0002-0.0005mol/LN- acetyl-D-Gln and 0.0001-is added in the sodium hydroxide solution
0.0002mol/LN- acetyl-L-glutamines；
The enzymatic hydrolysis condition is solid-liquid ratio 1:1.5-4,45-55 DEG C of temperature, pH8.5-9.5,8-12h of time, enzyme concentration
1500—2500U/g；
15-25wt% polyethylene Topiramate Los oxazolidinones and 0.5-0.9% tetradecanoic acid are added when the described freeze-drying.
3. a kind of purposes to safe and comfortable collagen peptide of the hepatic injury with repair, it is characterised in that the safe and comfortable skin
Collagen peptide is used for the treatment of non-alcoholic fatty liver disease.
4. having the purposes of the safe and comfortable collagen peptide of repair to hepatic injury according to claim 3, feature exists
In it be using safe and comfortable collagen peptide as active constituent, in addition acceptable auxiliary material or auxiliary on drug, food or health food
The preparation for including at least liquid preparation, solid pharmaceutical preparation or semisolid preparation that helping property ingredient is prepared.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810369285.4A CN108610413A (en) | 2018-04-23 | 2018-04-23 | A kind of safe and comfortable collagen peptide to hepatic injury with repair |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810369285.4A CN108610413A (en) | 2018-04-23 | 2018-04-23 | A kind of safe and comfortable collagen peptide to hepatic injury with repair |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108610413A true CN108610413A (en) | 2018-10-02 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810369285.4A Pending CN108610413A (en) | 2018-04-23 | 2018-04-23 | A kind of safe and comfortable collagen peptide to hepatic injury with repair |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108610413A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109295138A (en) * | 2018-09-27 | 2019-02-01 | 浙江海洋大学 | A kind of preparation method of anglerfish skin peptide and the immunological regulation application of prepared anglerfish skin peptide |
| CN114848768A (en) * | 2022-06-16 | 2022-08-05 | 何嗪 | Medicine for treating non-alcoholic fatty liver disease |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103627763A (en) * | 2013-11-14 | 2014-03-12 | 浙江省海洋开发研究院 | Method for grading molecular weight of anglerfish skin collagen peptide |
| CN104152518A (en) * | 2014-05-14 | 2014-11-19 | 浙江海洋学院 | Preparation method of hepatopathy complementary-food cod skin collagen peptide |
| CN106632605A (en) * | 2016-12-22 | 2017-05-10 | 浙江海洋大学 | Liver injury and repair type active peptide prepared from tuna offal |
-
2018
- 2018-04-23 CN CN201810369285.4A patent/CN108610413A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103627763A (en) * | 2013-11-14 | 2014-03-12 | 浙江省海洋开发研究院 | Method for grading molecular weight of anglerfish skin collagen peptide |
| CN104152518A (en) * | 2014-05-14 | 2014-11-19 | 浙江海洋学院 | Preparation method of hepatopathy complementary-food cod skin collagen peptide |
| CN106632605A (en) * | 2016-12-22 | 2017-05-10 | 浙江海洋大学 | Liver injury and repair type active peptide prepared from tuna offal |
Non-Patent Citations (1)
| Title |
|---|
| 东忠方 等: "安康鱼鱼皮胶原蛋白多肽酶法制备工艺研究", 《食品工业》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109295138A (en) * | 2018-09-27 | 2019-02-01 | 浙江海洋大学 | A kind of preparation method of anglerfish skin peptide and the immunological regulation application of prepared anglerfish skin peptide |
| CN109295138B (en) * | 2018-09-27 | 2021-09-17 | 浙江海洋大学 | Preparation method of anglerfish skin peptide and application of anglerfish skin peptide in immunoregulation |
| CN114848768A (en) * | 2022-06-16 | 2022-08-05 | 何嗪 | Medicine for treating non-alcoholic fatty liver disease |
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