CN108597335A - A kind of preparation method of green multifunctional teaching microchip - Google Patents
A kind of preparation method of green multifunctional teaching microchip Download PDFInfo
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- CN108597335A CN108597335A CN201810618370.XA CN201810618370A CN108597335A CN 108597335 A CN108597335 A CN 108597335A CN 201810618370 A CN201810618370 A CN 201810618370A CN 108597335 A CN108597335 A CN 108597335A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 238000013461 design Methods 0.000 claims abstract description 18
- 238000006243 chemical reaction Methods 0.000 claims abstract description 11
- 239000013078 crystal Substances 0.000 claims abstract description 9
- 229920001225 polyester resin Polymers 0.000 claims abstract description 8
- 239000004645 polyester resin Substances 0.000 claims abstract description 8
- 238000004809 thin layer chromatography Methods 0.000 claims abstract description 8
- 238000001259 photo etching Methods 0.000 claims abstract description 7
- 239000011159 matrix material Substances 0.000 claims abstract description 5
- 238000007711 solidification Methods 0.000 claims abstract description 5
- 230000008023 solidification Effects 0.000 claims abstract description 5
- 239000004205 dimethyl polysiloxane Substances 0.000 claims abstract 6
- 235000013870 dimethyl polysiloxane Nutrition 0.000 claims abstract 6
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 claims abstract 6
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 claims abstract 6
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims abstract 6
- 235000013405 beer Nutrition 0.000 claims abstract 2
- 238000004458 analytical method Methods 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 8
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 4
- 239000000463 material Substances 0.000 abstract description 3
- 230000001681 protective effect Effects 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 17
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 12
- 238000005516 engineering process Methods 0.000 description 12
- 239000000243 solution Substances 0.000 description 11
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 6
- 238000011160 research Methods 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 238000002347 injection Methods 0.000 description 4
- 239000007924 injection Substances 0.000 description 4
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 239000012286 potassium permanganate Substances 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 2
- 239000005864 Sulphur Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
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- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 description 2
- QPQKUYVSJWQSDY-CCEZHUSRSA-N 4-(phenylazo)aniline Chemical compound C1=CC(N)=CC=C1\N=N\C1=CC=CC=C1 QPQKUYVSJWQSDY-CCEZHUSRSA-N 0.000 description 1
- 239000004831 Hot glue Substances 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 206010022524 Intentional self-injury Diseases 0.000 description 1
- 239000007832 Na2SO4 Substances 0.000 description 1
- 208000005560 Self Mutilation Diseases 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- DMLAVOWQYNRWNQ-UHFFFAOYSA-N azobenzene Chemical compound C1=CC=CC=C1N=NC1=CC=CC=C1 DMLAVOWQYNRWNQ-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000019994 cava Nutrition 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 125000000118 dimethyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 238000005553 drilling Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229920005573 silicon-containing polymer Polymers 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000019345 sodium thiosulphate Nutrition 0.000 description 1
- 229910000679 solder Inorganic materials 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- DHCDFWKWKRSZHF-UHFFFAOYSA-N sulfurothioic S-acid Chemical compound OS(O)(=O)=S DHCDFWKWKRSZHF-UHFFFAOYSA-N 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
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- G—PHYSICS
- G09—EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
- G09B—EDUCATIONAL OR DEMONSTRATION APPLIANCES; APPLIANCES FOR TEACHING, OR COMMUNICATING WITH, THE BLIND, DEAF OR MUTE; MODELS; PLANETARIA; GLOBES; MAPS; DIAGRAMS
- G09B23/00—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
- G09B23/24—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for chemistry
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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Abstract
The present invention discloses a kind of preparation method of green multifunctional teaching microchip, includes the following steps:1) coreldraw Software for Design chips are used, by the required chip micro-structure Integrated design of the contents of courses such as thin-layer chromatography, the primary Beer laws of Lang, chemical reaction rate on chip;2) with common litho machine photoetching, prepare chip former;3) it is cast on the mold with PDMS, prepares PDMS chip formpistons after its solidification, and be assembled into final formpiston;4) it is poured on final formpiston finally by polyester resin and obtains crystal matrix teaching microchip;Microchip produced by the present invention has the following advantages:1, chip smaller, consumption reagent are less environmentally protective;2, the function of integrated chip is more;3, due to using common litho machine, the cost of manufacture of Chip mold relatively low;4, the improvement on mold design so that the material of chip manufacturing can be changed to more inexpensive polyester resin, therefore chip it is total production cost it is lower.
Description
Technical field
The invention belongs to chemistry teaching fixture technology fields, and in particular to a kind of preparation of green multifunctional teaching microchip
Method.
Background technology
This technology belongs to the crossing domain of micromation chemical reaction equipment and microfluidic chip technology, and main thought is profit
With the manufacturing technology of micro-fluidic chip, new microization Teaching of Analytical Chemistry utensil is made, Manz A et al. were in 90 years 20th century
In generation, has just been put forward for the first time the concept of micro-full analytical system (miniaturized total analysis system, μ-TAS).
Micro-fluidic chip therein combine analytical chemistry, MEMS (mi-cro-electro-mechanical system,
MEMS), the multidisciplinary field such as computer, materialogy, biomedicine, by such as sample pretreatment of the various functions of chemical laboratory,
Sample introduction, separation and detection etc., are integrated on the chip of credit card-sized, realize the micromation in laboratory, can greatly shorten whole
A analysis process required time;The consumption of reagent can be also reduced to microlitre even nanoliter level, a variety of analyses may be implemented
Function.Since the beginning of this century, microfluidic chip technology is developed rapidly, and has been widely used for chip capillary electricity
Swimming, materials synthesis, immunoassay, cell operation, crystallization of protein research etc. various fields, be analysis science research hot spot it
One.But the technology is presently mainly to apply in terms of scientific research, teaching and research aspect is using less, however microchip method consumptive material is few, just
The features such as property taken is good exactly meets the requirement of Green Chemistry, Microscale chemical experiment, is also convenient for realizing teaching combining theory with practice, because
This its in teaching field also there is very extensive application prospect.On the other hand, the technology such as MEMS skills that microchip makes at present
Art etc. costly, which also limits its teaching field application.
Microscale experiment is to emerge the 1980s, the generally acknowledged chemical experiment that can embody green chemical concept in the world
New technology and method.It, which has, saves drug, the advantages that saving the time, reduce " three wastes " of experiment, causes both at home and abroad
The most attention of experimental teaching personnel.But in general miniaturized devices need remain for the facilities such as mating water power, it can not
It really brings classroom into be tested, while instrument and equipment needs are special therefore also more expensive in price, cannot achieve teacher or even
The raw self-mutilation behavior to reaction unit.
Therefore, how the technology of the concept of Microscale chemical experiment and micro-fluidic chip to be combined together, designs religion
Microchip is that significantly, if scientific research microchip is directly used in teaching, its is of high cost, and often exists existing
As unobvious, the shortcomings of specific aim is poor, based on this, we, which design, has invented three generations and has imparted knowledge to students microchip, and micro flow chip technology is introduced
Teaching field has certain novelty, has applied for one Publication No. CN106563516A of Chinese invention patent, utility model patent
Three:Publication number is respectively CN206293074U, CN206285925U and CN206441437U, but due to these sides of technology restriction
Chip prepared by method is relatively rough, and has a single function, and can only use the relatively good easily peelable dimethyl silicone polymer of the transparency
(PDMS) it builds, therefore cost is higher.
Invention content
According to the above-mentioned deficiencies of the prior art, the technical problem to be solved by the present invention is to propose a kind of green multifunctional
The preparation method of teaching microchip, in order to solve the above-mentioned technical problem, the technical solution adopted by the present invention is:
A kind of preparation method of green multifunctional teaching microchip, includes the following steps:
1) coreldraw Software for Design chips are used, by teaching such as primary-Beer laws of thin-layer chromatography, Lang, chemical reaction rates
The required chip micro-structure Integrated design of content is on chip.
2) with common litho machine photoetching, prepare chip former.
3) it is cast on the mold with PDMS, PDMS chip formpistons is prepared after its solidification, and different by assembling
PDMS blocks obtain above-mentioned micro-structure, obtain the final formpistons of PDMS.
4) it is poured on above-mentioned final formpiston finally by polyester resin and obtains crystal matrix teaching microchip.
5) in the crystal film sealing-in step 4) for designing punch position using other one piece microchip surface, obtain core
Piece ontology.
Preferably, the micro-structure of the thin-layer chromatography is developing tank.
The micro-structure of preferred , Suo Shu primary-Beer laws of Lang is thickness gradient.
Preferably, the micro-structure of the chemical reaction rate is different height microflute.
Compared with prior art, beneficial effects of the present invention:
1. with common litho machine and coreldraw Software for Design and preparing chip so that chip manufacturing broken away from second,
Hand-made in third generation method, manufacturing process is more quick and specification, and the cost of manufacture of Chip mold is relatively low, while chip
Pipeline also greatly reduce, therefore more save reagent, have the advantages that it is environmentally protective, with the more our legal system of printing chip method
Standby pipeline is unlikely to deform and caves in, and prepared by chip has higher success rate.
2. the chip size prepared is small, good portability, classroom can be carried to and carry out teaching combining theory with practice, it is convenient same
Understand the content of courses in time, relatively obviously can more classmate be allowed intuitively to observe phenomenon with current teaching method.
3. works pass through the structure collection of a variety of teaching microchips by the early development of this seminar of smart structural design
At having arrived on chip piece so that it can be respectively used in the teaching such as primary-Beer laws of thin-layer chromatography, Lang, chemical reaction rate
Hold the purposes for improving chip, the function of integrated chip is more, increases the practicability of chip.
4. what works were obtained by photoetching is former, PDMS formpistons are obtained after copying pattern by PDMS, which can
The microchip of above-mentioned crystal matrix is prepared with Reusability, chip body finally prepd in this way is polyester resin, and polyester resin
It is cheap, and prepared by other several generations methods is formpiston, it, can not be from the mold constructed due to harder after crystal gum forming
Stripping, and PDMS glue is then peelable, therefore finally prepd chip body is PDMS, PDMS is expensive, so in general
This works obtains chip will be cheap than other several generations products.
Description of the drawings
1. Fig. 1 is the structural design drawing of microchip in embodiment;
2. Fig. 2 is the making pictorial diagram of microchip in embodiment;
3. Fig. 3 be in embodiment when microchip operation concentration gradient (or different proportion reagent) formed schematic diagram (with
For liquor potassic permanganate);
1,2 injection port, 3-7, lower end water outlet, 8, Lang primary-Beer law micro-structures, 9, be thin-layer chromatography micro-structure, a,
One-dimensional photoetching glue pattern plate (former) b, injects PDMS, after c, PDMS solidification in cavity after the template is clamped with vertical clamp
The one-dimensional PDMS formpistons obtained are removed, d, the thickness gradient in 1 region and the different height in 2 regions are micro- in PDMS template over-assembles
The three-dimensional PDMS formpistons formed after slot both final template.
Specific implementation mode
It below by the description to embodiment, is described in further detail, to help those skilled in the art to this hair
Bright inventive concept, technical solution have more complete, accurate and deep understanding.
Embodiment 1
With reference to attached drawing 1,2,3, the present invention provides a kind of green multifunctional teaching microchip preparation method, uses
Coreldraw Software for Design templates, litho machine photoetching, PDMS make final template, and polyester resin makes chip, including following step
Suddenly:
1) coreldraw Software for Design chips are used, by thin-layer chromatography (micro-structure:Developing tank primary-Beer law of), Lang is (micro-
Structure:Thickness gradient), chemical reaction rate (micro-structure:Different height microflute) etc. the required chip micro-structure of the contents of courses
Integrated design is on chip.
2) with common litho machine photoetching, prepare chip former.
3) it is cast on the mold with PDMS, PDMS chip formpistons is prepared after its solidification, and different by assembling
PDMS blocks obtain above-mentioned micro-structure, obtain the final formpistons of PDMS.
4) it is poured on above-mentioned final formpiston finally by polyester resin and obtains crystal matrix teaching microchip.
5) in the crystal film sealing-in step 4) for designing punch position using other one piece microchip surface, obtain core
Piece ontology.
As shown in figure 3, a kind of template for microchip of imparting knowledge to students for green multifunctional comprising sample channel 1 and water inlet line
2,0.1mol/L potassium permanganate and water are injected from 1 and 2 pipelines in chip respectively can bring out mouth of a river 3-7 under the die forms as schemed
Concentration gradient.In addition in the design, each go out sample outlet connection reservoir there are certain thickness gradients, both each
The thickness of reservoir is incremented by successively below out sample tube road, in respectively 1mm, 2mm, 3mm such as Fig. 2 d 3 illustrate.
Principle using Lambert-Beer's law microchip for teaching is as follows:By certain density potassium permanganate and pure water
It is injected in chip from injection port 1 and 2 respectively, to form the concentration gradient of potassium permanganate in the outlet in five out sample tube roads,
Meanwhile in the exit in any one out sample tube road, the different-thickness reservoir being arranged in order will form the absorption cell of different-thickness.
The depth is will present in the color of absorption due to liquor potassic permanganate to light, various concentration gradient and different absorption cell thickness
Variation, to help classmate to understand the absorbance of solution pass directly proportional to solution concentration and absorption cell thickness by this microchip
System's both Lambert-Beer's law.
Embodiment 2
The present embodiment is with embodiment 1, the difference is that the progress of this chip is chemical reaction rate teaching, concrete operations are such as
Under:
The hypo solution of 0.5mol/L and pure water are injected to from injection port 1 and 2 in chip respectively, thiosulfuric acid
Out sample tube road is flowed by the pipeline that crosses after sodium solution and pure water mixing, passes through friendship after being mixed with pure water because of hypo solution
Header road arrives separately at the stroke difference that each out sample tube road is undergone, to form various concentration ladder in five out sample tube roads
The hypo solution of degree, while micro- heating device is placed into heating at the 4 deeper channel positions illustrated in figure 2d so that should
Slot position temperature increases, and the sulfuric acid solution of 0.1mol/L is added in advance in each group reservoir, will occur in this way in each slot as follows
Reaction:Na2S2O3+H2SO4==Na2SO4+S↓+SO2+H2O
Because hypo solution concentration is different with the temperature of slot in the reservoir in any one out sample tube road exit, from
And be observed that in each row, the speed for sulphur precipitation occur of concentration of sodium thiosulfate greatly is fast, while the storage in each row after heating
The speed for occurring sulphur precipitation in liquid bath is fast, and the pass of chemical reaction rate and concentration and temperature will be run away with by observing classmate
System.
Embodiment 3
The present embodiment is with embodiment 1, the difference is that the progress of this chip is thin-layer chromatography teaching, concrete operations are as follows:
Chloroform and benzene are injected to from injection port 1 and 2 in chip respectively, pass through the pipeline that crosses after chloroform and benzene mixing
It flows into out sample tube road, because respectively reaching the stroke that each out sample tube road is undergone by the pipeline that crosses after chloroform and ethyl alcohol mixing
Difference, to the Mixed Expansion agent of the chloroform and benzene of formation different proportion in five out sample tube roads;Meanwhile in each group reservoir
Pit (in Fig. 19 regions formed slot position) addition point has the thin-layer tablet of azobenzene, P-aminoazobenzene, above-mentioned difference in advance
The Mixed Expansion agent of the chloroform and benzene of ratio enters preceding slot (the 8 region shape in Fig. 1 of reservoir behind the outlet for reaching out sample tube road
At slot position), saturation picks up microchip inclination and pours into Mixed Expansion agent in pit after twenty minutes, to from the expansion knot of pit
Fruit can see the Rf value R that sample is unfolded in the Mixed Expansion agent of different proportionfThe influence of value.
The product and former generation product Integrated comparative of the present invention is shown in Table 1:
Table 1:Four generation product Integrated comparatives
Note 1:Every 100 totle drilling cost calculated examples 1000+0.5+10 × 100=2000.5
This seminar has developed a variety of teaching microchip production methods:First generation printing chip method, second generation weldering
Tin silk builds method, and third generation hot melt adhesive builds method.These methods for a certain knowledge point design and make chip, and using it is low at
The strong innovation of this structure, but all there is also some defects, if printing chip method pipeline is thinner, are easy to happen fracture, experiment is existing
As unobvious, solder stick and thermosol method are required to hand-made therefore time-consuming and laborious.And what all these methods were eventually fabricated
Chip can only be PDMS, and costly which has limited the applications of the above method for PDMS prices.
The present invention is exemplarily described above in conjunction with specific embodiment, it is clear that the present invention implements not by upper
The limitation of mode is stated, if the improvement of the various unsubstantialities of inventive concept and technical scheme of the present invention progress is used, or
It is not improved by the present invention design and technical solution directly apply to other occasions, protection scope of the present invention it
It is interior.Protection scope of the present invention should be determined by the scope of protection defined in the claims.
Claims (4)
1. a kind of preparation method of green multifunctional teaching microchip, which is characterized in that specific preparation method is as follows:
1) coreldraw Software for Design chips are used, by contents of courses such as primary-Beer laws of thin-layer chromatography, Lang, chemical reaction rates
Required chip micro-structure Integrated design is on chip;
2) with common litho machine photoetching, prepare chip former;
3) it is cast on the mold with PDMS, PDMS chip formpistons is prepared after its solidification, and by assembling different PDMS blocks
Different micro-structures are obtained, the final formpistons of PDMS are obtained;
4) it is poured on above-mentioned final formpiston finally by polyester resin and obtains crystal matrix teaching microchip;
5) in the crystal film sealing-in step 4) for designing punch position using other one piece microchip surface, obtain chip sheet
Body.
2. the preparation method of green multifunctional teaching microchip according to claim 1, which is characterized in that the thin layer
The micro-structure of analysis is developing tank.
3. the preparation method of green multifunctional teaching microchip according to claim 1, which is characterized in that Suo Shu Lang primary-
The micro-structure of Beer law is thickness gradient.
4. the preparation method of green multifunctional teaching microchip according to claim 1, which is characterized in that the chemistry is anti-
It is different height microflute to answer the micro-structure of rate.
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Cited By (1)
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CN109490463A (en) * | 2018-11-12 | 2019-03-19 | 安徽中医药高等专科学校 | A kind of chromatography teaching aid and preparation method thereof |
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