CN108587902A - Cell sorting devices based on dielectrophoresis and its screening technique - Google Patents
Cell sorting devices based on dielectrophoresis and its screening technique Download PDFInfo
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Abstract
The present invention relates to a kind of cell sorting devices based on dielectrophoresis, belong to cell filtration sorting technology field.The device includes ito glass basal layer, 3D electrode layers, PDMS cover plate layers.Designed 3D electrode layers are made of two rows of electrodes, wherein every four small cuboid electrode is corresponding with big cuboid electrode to be arranged in chip channel both sides, to generate electric field heterogeneous.Pass through the design to electrode, utilize dielectrophoretic force, make target microcell by positive dielectrophoretic force, untargeted cells makes sample cell do " sine curve " flowing in split tunnel by negative dielectrophoretic force, by the device, when sample cell is close to small cuboid electrode, target cell is by stronger positive dielectrophoretic force, it will be flowed out along the runner close to electrode, to make target cell more preferably be screened from sample cell, realize efficiently separating for cell.
Description
Technical field
The invention belongs to cell filtration sorting technology fields, are related to a kind of cell multistage screening dress based on dielectrophoresis effect
It sets and its screening technique.
Background technology
Micro-fluidic chip obtains more and more concerns in every field in recent years, for example, chemical analysis, unicellular
The fields such as analysis, medical diagnosis and organizational project.Its huge advantage is simple and quick process, when shortening analysis
Between, it detects the high sensitivity of small amount sample and can realize multifunctional integrated etc..And based on micro-fluidic chip to biology
Grain or cell are manipulated and are detached, and are had in fields such as biologic medical, clinical diagnosis, food Bacteria Detection and environmental monitorings
There is important influence, thus has obtained the research of numerous scholars.
Method based on micro-fluidic progress particle or cell separation generally includes optics, magnetic field, sound field and electric field side
Method, and in electric field methods, the method for dielectrophoresis (Dielectrophoresis, DEP) be widely used in micro-nano granules or
The separation of person's cell.Dielectrophoresis refers to the different characteristics based on particle and solution, movement of the particle under inhomogeneous field.So
And when required separation micro-nano granules or few cell content, dielectrophoresis separation method is faced with that integrated level is low, capture rate is low
Defect.
Invention content
To solve the above problem of the existing technology, the present invention provides a kind of cell sorting devices based on dielectrophoresis
(separating chips), by designing electric field heterogeneous, using dielectrophoretic force, when sample cell being made to do " sine curve " flowing, from
In filter out target cell.
To achieve the goals above, technical scheme is as follows：A kind of cell sorting devices based on dielectrophoresis, packet
Include ito glass basal layer, 3D electrode layers, PDMS cover plate layers；
The ito glass basal layer is equipped with two rows of ITO electrodes, including ITO electrode I and ITO electrode II, can be as electricity
Conducting wire between source line and 3D electrode layers；
The 3D electrode layers accumulation is connected in ito glass basal layer with the ITO electrode of ito glass basal layer setting；It is described
PDMS cover plates layer is bonded with the ito glass basal layer of accumulation 3D electrode layers；
The 3D electrode layers are made of two rows of 3D electrodes, wherein a row 3D electrodes are by second group of 3D electrode, the 4th group of 3D electricity
Pole, the 6th group of 3D electrode, the 8th group of 3D electrodes composition；Another row 3D electrodes are by first group of 3D electrode, third group 3D electrodes, the 5th
Group 3D electrodes, the 7th group of 3D electrodes composition, two rows of 3D electrodes of 3D electrode layers two rows with ito glass basal layer respectively
ITO electrode is correspondingly connected with；
Described PDMS cover plates layer one end is equipped with pumps the round entrance chamber being connected with liquid injection, and centre is split tunnel, separately
One end is equipped with round exit chamber I, round exit chamber II and round exit chamber III, the round entrance chamber, round exit chamber I, circle
Shape outlet plenum II and round exit chamber III are opened type.
The wherein every four small cuboid electrode of two rows of 3D electrodes of the 3D electrode layers is corresponding with big cuboid electrode
Split tunnel both sides are arranged in, to generate electric field heterogeneous.
The ITO electrode I is obtained with ITO electrode II by being retained after the ITO conduction erosions of ito glass substrate surface；Institute
It is 200nm that ITO electrode I, which is stated, with II thickness of ITO electrode.
First group of 3D electrode, the 4th group of 3D electrode, the 5th group of 3D electrode, the 8th group of 3D electrode are in the 3D electrode layers
It is made of the identical four small cuboid electrode of size；Second group of 3D electrode, third group 3D electrodes, the 6th group of 3D electrodes, the 7th
Group 3D electrodes be by identical big cuboid electrode group at.
The top surfaces of the 3D electrode layers 4, side and connect below with PDMS, before split tunnel 18 together as conduit wall
A part.
Spacing is 70um~150um between two rows 3D electrodes in the 3D electrode layers, and spacing is between every group of 3D electrode
70um~200um, 3D electrode layers thickness 30um~60um.
Each small cuboid in first group of 3D electrode, the 4th group of 3D electrode, the 5th group of 3D electrode, the 8th group of 3D electrode
The wide 70um of electrode~120um long 1mm~2mm, spacing 70um~120um between small cuboid electrode.Second group of 3D electrodes, third
Wide 490um~840um long 1mm~2mm of group 3D electrodes, the 6th group of 3D electrode, the 7th group of 3D electrode.
The round exit chamber I and round exit chamber III are symmetrically disposed on the both sides of outlet plenum II.
The present invention also provides a kind of screening techniques using above-mentioned apparatus, include the following steps：
A, cell sample solution is put into centrifuge tube through centrifugal treating, buffer solution is added, shakes up, repeatedly, obtains
To cell suspension, which is diluted to required concentration with test buffer；
B, by being pumped into appropriate test buffer in syringe pump, the round entrance chamber of separating chips, note are connected by conduit
Enter in split tunnel, empty split tunnel in air, to prevent on 3D electrodes, microchannel edge formed bubble；
C, by being pumped into appropriate treated cell suspension in syringe pump, pass through the round entrance that conduit connects separating chips
Chamber injects in split tunnel, while open signal source, by adjusting voltage and frequency, make target cell from round exit chamber I,
Round exit chamber III flows out, and untargeted cells is from round exit chamber II, to realize efficiently separating for cell.
Compared with prior art, the present invention has the beneficial effect that：
By the design to electrode, electric field heterogeneous is generated, using dielectrophoretic force, makes target microcell by positive dielectrophoresis
Power, untargeted cells makes sample cell do " sine curve " flowing by negative dielectrophoretic force, by multilevel screening device, thin in sample
When born of the same parents lean on small cuboid electrode, target cell flows out, to make mesh by stronger positive dielectrophoretic force along the runner close to electrode
Mark cell is more preferably screened from sample cell, realizes efficiently separating for cell.The present invention overcomes to the few target of content
When cell is detached, the defects such as the integrated level of dielectrophoresis separation method is low, capture rate is low.In addition, the chip structure of this system
Simply, easy to process, have many advantages, such as easy to operate, high degree of automation, can be used for rare cell biological study, disease morning
The fields such as phase Clinics and Practices.
Description of the drawings
The present invention shares 4 width of attached drawing, wherein：
Fig. 1 is the cell sorting devices overall structure diagram based on dielectrophoresis.
Fig. 2 is the structural schematic diagram of ito glass basal layer.
Fig. 3 is the structural schematic diagram of 3D electrode layers.
Fig. 4 is the structural schematic diagram of PDMS cover plate layers.
Wherein：1, ito glass basal layer, 2, ITO electrode I, 3, ITO electrode II, 4,3D electrode layers 4,5, first groups of 3D electricity
Pole, 6, second groups of 3D electrodes, 7, third group 3D electrodes, 8, the 4th groups of 3D electrodes, 9, the 5th groups of 3D electrodes, 10, the 6th groups of 3D electricity
Pole, 11, the 7th groups of 3D electrodes, 12, the 8th groups of 3D electrodes, 13, PDMS cover plate layers, 14, round entrance chamber, 15, round exit chamber
I, 16, round exit chamber II, 17, round exit chamber III, 18, split tunnel.
Specific implementation mode
Following further describes the present invention with reference to the drawings；The non-limiting embodiment can make this field below
Those of ordinary skill the present invention, but do not limit the invention in any way is more fully understood.
As shown in Figs 1-4, a kind of cell sorting devices based on dielectrophoresis, including ito glass basal layer 1,3D electrode layers
4, PDMS cover plates layer 13；
The ito glass basal layer 1 is equipped with two rows of electrodes, the method for using wet etching using ito thin film on ito glass
It is made, including ITO electrode I 2 and ITO electrode II 3, as the conducting wire between power cord and 3D electrode layers 4；
4 accumulation of 3D electrode layers is connected in ito glass basal layer 1 with the ITO electrode of ito glass basal layer 1；It is described
PDMS cover plates layer 13 is bonded with the ito glass basal layer 1 of accumulation 3D electrode layers 4；
The 3D electrode layers 4 by cleaning, press mold, exposure, development operating method, make dry film during electrode machining
It is used as cavity plate, is used for the filling of Ag PDMS, is then formed by curing 3D electrode structures；Wherein a row 3D electrodes are by second group
3D electrodes 6, the 4th group of 3 electrode 8, the 6th group of 3D electrode 10, the 8th group of 3D electrode 12 form；Another row 3D electrodes are by first group of 3D
Electrode 5,7, the 5th groups of 3D electrodes 9 of third group 3D electrodes, the 7th group of 3D electrode 11 form, two rows of 3D electricity of the 3D electrode layers 4
Pole is correspondingly connected with two rows of ITO electrodes of ito glass basal layer 1 respectively；
The PDMS cover plates layer 13 is that the method transferred after first photoetching is made, and described 13 one end of PDMS cover plates layer is equipped with
The round entrance chamber 14 being connected is pumped with liquid injection, centre is split tunnel 18, and the other end is equipped with round exit chamber I 15, circle
Outlet plenum II 16 and round exit chamber III 17, the round entrance chamber 14, round exit chamber I 15, round exit chamber II 16 and circle
Shape outlet plenum III 17 is opened type.
The ITO electrode I 2 and II 3 thickness of ITO electrode are 200nm.
First group of 3D electrode 5, the 4th group of 3D electrode 8, the 5th group of 3D electrode 9, the 8th group of 3D electrode in the 3D electrode layers 4
12 are made of the identical four small cuboid electrode of size；7, the 6th groups of second group of 3D electrode 6, third group 3D electrodes 3D electricity
Pole 10, the 7th group of 3D electrode 11 be by the identical one big cuboid electrode group of size at.
Spacing is 100um between two rows 3D electrodes in the 3D electrode layers 4, and spacing is 100um, 3D between every group of 3D electrode
4 thickness 30um of electrode layer.
It is each small in first group of 3D electrode 5, the 4th group of 3D electrode 8, the 5th group of 3D electrode 9, the 8th group of 3D electrode 12
The wide 100um long 2mm of cuboid electrode, spacing 100um between small cuboid electrode；Second group of 3D electrode 6, third group 3D electrodes 7,
The wide 700um long 2mm of 6th group of 3D electrode 10, the 7th group of 3D electrode 11.
The round exit chamber I and round exit chamber III are symmetrically disposed on the both sides of outlet plenum II.
A kind of cell screening method based on dielectrophoresis, includes the following steps：
A, cell sample solution is put into centrifuge tube through centrifugal treating, buffer solution is added, shakes up, repeatedly, obtains
To cell suspension.The cell suspension is diluted to required concentration with test buffer.
B, by being pumped into appropriate test buffer in syringe pump, the round entrance chamber 14 of separating chips is connected by conduit,
Inject split tunnel 18 in, empty split tunnel 18 in air, to prevent on 3D electrodes, microchannel edge formed bubble.Liquid
Flow velocity should not be too large also should not be too small, and flow velocity, which crosses conference, causes air pressure moment in channel to increase, to destroy PDMS cover plates layer 13
With being bonded for ito glass basal layer 1.If instead the flow velocity too small plenty of time that can make fluid injection wastes in the catheter.
C, by being pumped into appropriate treated cell suspension in syringe pump, pass through the round entrance that conduit connects separating chips
Chamber 14 injects in split tunnel 18.Open signal source simultaneously makes target cell from round exit by adjusting voltage and frequency
Chamber I 15, round exit chamber III 17 flow out, and untargeted cells is from round exit chamber II 18, to realize efficiently separating for cell.
One embodiment of the present of invention is as follows：
The circulating tumor cell separation method of the present invention, includes the following steps：
1, patient with breast cancer's 3ml blood sample solutions are put into centrifuge tube through centrifugal treating, PBS buffer solution are added,
It shakes up, in triplicate, obtains cell suspension.The cell suspension is diluted to institute with 8.5% sucrose, 0.3% dextrose buffer liquid
Need concentration.
2, it is pumped into appropriate 8.5% sucrose, 0.3% dextrose buffer liquid in syringe pump, separating chips are connected by conduit
Round entrance chamber 14 is injected in split tunnel 18, is flowed from round exit chamber 154, round exit chamber 16 with round exit chamber 17
Go out, empty split tunnel 18 in air, to prevent on 3D electrodes, microchannel edge formed bubble.Sieve channel is rushed simultaneously, is prevented thin
Born of the same parents adhere to conduit wall.
3, by being pumped into treated cell suspension in syringe pump, pass through the round entrance chamber that conduit connects separating chips
14, it injects in split tunnel 18.By adjusting voltage and frequency, make breast cancer cell by positive dielectrophoretic force, blood cell is by negative
Dielectrophoretic force, final breast cancer cell are flowed out from round exit chamber I 15 with round exit chamber III 17, and blood cell goes out from circle
It flows out in oral cavity II 16.Breast cancer cell is efficiently separated to realize.
It is completed by above step and breast cancer cell in blood is efficiently separated.
Claims (6)
1. a kind of cell sorting devices based on dielectrophoresis, it is characterised in that：Including ito glass basal layer (1), 3D electrode layers
(4), PDMS cover plates layer (13)；
The ito glass basal layer (1) is equipped with two rows of ITO electrodes, including ITO electrode I (2) and ITO electrode II (3), as electricity
Conducting wire between source line and 3D electrode layers (4)；
3D electrode layers (4) accumulation is connected in ito glass basal layer (1) with the ITO electrode of ito glass basal layer (1)；Institute
PDMS cover plates layer (13) is stated to be bonded with the ito glass basal layer (1) of accumulation 3D electrode layers (4)；
The 3D electrode layers (4) are made of two rows of 3D electrodes, wherein a row 3D electrodes are by second group of 3D electrode (6), the 4th group of 3 electricity
Pole (8), the 6th group of 3D electrode (10), the 8th group of 3D electrodes (12) composition；Another row 3D electrodes are by first group of 3D electrode (5),
Three groups of 3D electrodes (7), the 5th group of 3D electrode (9), the 7th group of 3D electrodes (11) composition, two rows of 3D electricity of the 3D electrode layers (4)
Two rows of ITO electrodes of the pole respectively with ito glass basal layer (1) are correspondingly connected with；
Described PDMS cover plates layer (13) one end is equipped with pumps the round entrance chamber (14) being connected with liquid injection, and centre is split tunnel
(18), the other end is equipped with round exit chamber I (15), round exit chamber II (16) and round exit chamber III (17), and the circle enters
Oral cavity (14), round exit chamber I (15), round exit chamber II (16) and round exit chamber III (17) are opened type.
2. the cell sorting devices based on dielectrophoresis according to claim 1, it is characterised in that：The ITO electrode I (2) with
ITO electrode II (3) thickness is 200nm.
3. the cell sorting devices based on dielectrophoresis according to claim 2, it is characterised in that：In the 3D electrode layers (4)
First group of 3D electrode (5), the 4th group of 3D electrode (8), the 5th group of 3D electrode (9), the 8th group of 3D electrode (12) are by size phase
Same four small cuboid electrode composition；Second group of 3D electrode (6), third group 3D electrodes (7), the 6th group of 3D electrode (10),
Seven groups of 3D electrodes (11) be by identical big cuboid electrode group at.
4. the cell sorting devices based on dielectrophoresis according to claim 1, it is characterised in that：In the 3D electrode layers (4)
Spacing is 70um~150um between two rows of 3D electrodes, and spacing is 70um~200um between every group of 3D electrode, and 3D electrode layers (4) are thick
Spend 30um~60um.
5. the cell sorting devices based on dielectrophoresis according to claim 1, it is characterised in that：First group of 3D electrode
(5), each small wide 70um of cuboid electrode in the 4th group of 3D electrode (8), the 5th group of 3D electrode (9), the 8th group of 3D electrode (12)
~120um long 1mm~2mm, spacing 70um~120um between small cuboid electrode；Second group of 3D electrode (6), third group 3D electricity
Pole (7), the 6th group of 3D electrode (10), the 7th group of 3D electrode (11) wide 490um~840um long 1mm~2mm.
6. a kind of screening technique using the device described in any one of above-mentioned 1-5, it is characterised in that：Include the following steps：
A, cell sample solution is put into centrifuge tube through centrifugal treating, buffer solution is added, shakes up, repeatedly, obtained thin
The cell suspension is diluted to required concentration by born of the same parents' suspension with test buffer；
B, by being pumped into test buffer in syringe pump, the round entrance chamber (14) of separating chips, injection point are connected by conduit
From in channel (18), the air in split tunnel (18) is emptied；
C, by being pumped into treated cell suspension in syringe pump, the round entrance chamber (14) of separating chips is connected by conduit,
It injects in split tunnel (18), while open signal source, by adjusting voltage and frequency, makes target cell from round exit chamber I
(15), round exit chamber III (17) flows out, and untargeted cells is from round exit chamber II (18), to realize the separation of cell.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110102183A (en) * | 2019-05-14 | 2019-08-09 | 青岛大学 | A kind of dielectrophoresis method high-efficiency and continuous separation micro particles new process |
| CN110272823A (en) * | 2019-07-05 | 2019-09-24 | 大连海事大学 | A kind of many cells surface portion region magnetizing assembly and method based on micro channel array |
| CN110272811A (en) * | 2019-07-05 | 2019-09-24 | 大连海事大学 | A kind of unicellular surface portion region magnetizing assembly and method based on twin columns capture |
| CN113181980A (en) * | 2021-04-13 | 2021-07-30 | 大连海事大学 | Micro plastic particle separation device and method based on direct current bias alternating current electric field |
| CN113189180A (en) * | 2021-03-29 | 2021-07-30 | 大连海事大学 | Microalgae characterization and identification device and method based on alternating current-dielectrophoresis |
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| WO2021198414A1 (en) * | 2020-04-01 | 2021-10-07 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung eingetragener Verein | Object with active anti-adhesive surface |
| WO2021239010A1 (en) * | 2020-05-26 | 2021-12-02 | 中国科学院青岛生物能源与过程研究所 | Cell sorting chip, apparatus, and method based on dielectric deterministic displacement |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101234292A (en) * | 2007-11-09 | 2008-08-06 | 东南大学 | Direction controllable microfluid dielectrophoresis granule separating device |
| JP2009250706A (en) * | 2008-04-03 | 2009-10-29 | Canon Inc | Method and apparatus for separating particles |
| CN102302898A (en) * | 2011-06-17 | 2012-01-04 | 西安交通大学 | Micro blood cell separation device and method for using same |
| CN104307638A (en) * | 2014-08-19 | 2015-01-28 | 阮海生 | Efficient DEP electrode structure, plate electrode formed therefrom and electrode array |
| KR20150106784A (en) * | 2014-03-12 | 2015-09-22 | 국립대학법인 울산과학기술대학교 산학협력단 | Dielectrophoresis based particle separator using serpentine channel |
| CN105647799A (en) * | 2016-02-24 | 2016-06-08 | 西安交通大学 | Circulating tumor cell separation device adopting combined field flow separation |
| CN107115897A (en) * | 2016-02-25 | 2017-09-01 | 中国科学院苏州纳米技术与纳米仿生研究所 | Micro-fluidic chip and preparation method thereof |
| CN107267382A (en) * | 2017-07-26 | 2017-10-20 | 南方科技大学 | A kind of micro-fluidic chip based on dielectrophoresis and its preparation method and application |
-
2018
- 2018-06-01 CN CN201810557181.6A patent/CN108587902A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101234292A (en) * | 2007-11-09 | 2008-08-06 | 东南大学 | Direction controllable microfluid dielectrophoresis granule separating device |
| JP2009250706A (en) * | 2008-04-03 | 2009-10-29 | Canon Inc | Method and apparatus for separating particles |
| CN102302898A (en) * | 2011-06-17 | 2012-01-04 | 西安交通大学 | Micro blood cell separation device and method for using same |
| KR20150106784A (en) * | 2014-03-12 | 2015-09-22 | 국립대학법인 울산과학기술대학교 산학협력단 | Dielectrophoresis based particle separator using serpentine channel |
| CN104307638A (en) * | 2014-08-19 | 2015-01-28 | 阮海生 | Efficient DEP electrode structure, plate electrode formed therefrom and electrode array |
| CN105647799A (en) * | 2016-02-24 | 2016-06-08 | 西安交通大学 | Circulating tumor cell separation device adopting combined field flow separation |
| CN107115897A (en) * | 2016-02-25 | 2017-09-01 | 中国科学院苏州纳米技术与纳米仿生研究所 | Micro-fluidic chip and preparation method thereof |
| CN107267382A (en) * | 2017-07-26 | 2017-10-20 | 南方科技大学 | A kind of micro-fluidic chip based on dielectrophoresis and its preparation method and application |
Non-Patent Citations (1)
| Title |
|---|
| 陈礼 等: "基于介电电泳的微流控细胞分离芯片的研究进展", 《分析化学》 * |
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|---|---|---|---|---|
| CN110102183A (en) * | 2019-05-14 | 2019-08-09 | 青岛大学 | A kind of dielectrophoresis method high-efficiency and continuous separation micro particles new process |
| CN110272823A (en) * | 2019-07-05 | 2019-09-24 | 大连海事大学 | A kind of many cells surface portion region magnetizing assembly and method based on micro channel array |
| CN110272811A (en) * | 2019-07-05 | 2019-09-24 | 大连海事大学 | A kind of unicellular surface portion region magnetizing assembly and method based on twin columns capture |
| CN110272823B (en) * | 2019-07-05 | 2022-06-24 | 大连海事大学 | Multi-cell surface partial-area magnetizing device and method based on micro-channel array |
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