CN108570444A - ST cells tame suspension process and second order virus production technique - Google Patents
ST cells tame suspension process and second order virus production technique Download PDFInfo
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Abstract
The present invention relates to ST cells domestication suspension process and second order virus production techniques.Specifically, adapt it to serum free suspension culture the present invention relates to adherent ST cells are tamed, and using it as host cell, malicious mode is connect using second order culture and produces and lesion or there can be the virus of sensibility on suspension ST cells, that is, cell is grown to 2.0~20.0 × 106When cell/mL, 1~5 times is diluted with production medium, makes density 1.0~10.0 × 106Between cell/mL, poison is then connect, or poison is connect before dilution.The cell adapted suspension growths in the culture medium that serum-free and chemical composition determine of suspension ST of the present invention, second order virus production is simple for process, easily amplifies；Liquid is not changed, culture medium utilization ratio is high；Production and growth medium can be identical or different, and the two proportionally mixes, you can make cell diauxic growth and promote expressing viral.
Description
Technical field
The present invention relates to ST cells domestication suspension process and suspension ST cell related vaccines production fields, and in particular to 30L
Or more bioreactor culture suspension ST cells, with second order cultural method culture suspension ST cells to produce the technique of vaccine
Technology.
Background technology
Mammalian cell large-scale culture technology is one of Biopharmaceutical Enterprises downstream general technology, is sent out in the sector
Wave particularly important effect.The key of this technology is the suspension serum-free large-scale culture of realization mammalian cell, from
And production capacity is improved, cost is reduced, and be easy to amplify.Attached cell cultivate when usual way be add in the medium it is a certain amount of
Serum makes cell adherent growth, and serum chemistry ingredient is uncertain, and unstable quality, has difference between batch, is produced with such cell
Biological products, quality is difficult to control, it is difficult to by the examination ＆ approval of food and medicine Surveillance Authority, and suspension free serum culture is opposite
For common adhere-wall culture, unit volume can grow more cells, so as to produce more biological products.
ST cells are Pig testicular cell (swine testis), which has been widely used in swine fever virus (SFV), puppet
The separation of rabies viruses (PRV) and pig parvoviral (SFV) etc., the production of in-vitro multiplication and a variety of live vaccines.
The defect that existing ST cells domestication suspends is：1, some cannot achieve complete free serum culture；2, it generally adopts
The method walked with two steps is first reduced to serum-free settling flux culture, due to adhere-wall culture use culture medium it is usual based on train
Base increase serum is supported, basal medium nutritional ingredient is weaker, and adhere-wall culture surface area/volume ratio is usually smaller during domestication, limit
The intake of nutrition is made, time-consuming for entire domestication process；3, it is tamed after genetic modification, i.e., is attached cell by genetic modification
Strain, which is inserted into, is unfavorable for adherent genetic fragment, changes original adhere-wall culture mode, makes cell suspension growth, then passes through training again
Support continuing to optimize for base enables cell serum-free high-density growth with change, and such method is easy to implement, but inserts outer
Source genetic fragment influences the quality of product.Therefore, it is necessary to ST cells suspension domestication and serum-free acclimation method further grind
Study carefully, and overcomes the defect of above three aspect.
Malicious process aspect is connect in virus, after traditional viral vaccine production technology refers to cell growth to certain density,
Liquid is changed to connect poison again or directly connect poison.So far, not about the relevant report of the second order cultural method of suspension ST cells.
Invention content
The first aspect of the present invention is related to a kind of second order cultural method of suspension ST cell vaccines production, and it includes following steps
Suddenly：
1) cell growth：Suspension ST cells grow to 2.0 × 10 in growth medium6Cell/mL~20.0 × 106Carefully
Born of the same parents/mL；
2) poison is connect：Poison amount is connect 10-1~10-5Between MOI, or poison amount is connect according to this after step 3) and connect poison；
3) dilute/add production medium：1~5 times is diluted with production medium, cell density is to 1.0 × 10 after dilution6
Cell/mL~10.0 × 106Cell/mL；
4) diauxic growth：Cell diauxic growth；
5) optionally, harvest virus liquid purifies seedling.
In some embodiments, suspension ST cell growths are suspension tank culture, and training volume is 30L or more.
In some embodiments, in step 1) cell density 4.0 × 106Cell/mL~15.0 × 106Cell/mL,
Preferably, 5.0 × 106Cell/mL~12.0 × 106Cell/mL, most preferably, 10.0 × 106Cell/mL.
In some embodiments, cell connects poison amount 10 in step 2)-1~10-4Between MOI, more excellent ground connection, 10-1~
10-3MOI, optimal ground connection, 10-2MOI。
In some embodiments, suspension ST cell production mediums are diluted to 1~4 times in step 3), it is highly preferred that 1
~3 times of dilutions, most preferably 2 times dilutions.
In some embodiments, cell density is 1.0 × 10 after poison being connect in step 3)6Cell/mL~10.0 × 106Carefully
Born of the same parents/mL, it is preferable that 1.0 × 106Cell/mL~5.0 × 106Cell/mL.
In some embodiments, suspension ST cells are the suspension ST cells used in production of vaccine, or by adherent ST cells
Domestication, the suspension ST cells for capableing of suspension growth production, it is preferable that suspension ST cells are by adherent ST cells through low serum
Domestication or serum-free domestication, serum-free domestication refer to that ST cells after taming can be in the culture medium existing for serum-free
Growth, proliferation, and/or, it is preferable that suspension ST cells be by adherent ST cells through suspend domestication from.
In some embodiments, virus is the disease that is sensitive, can producing corresponding vaccine on corresponding suspension ST cells
Poison, it is preferable that virus is selected from swine fever virus, Pseudorabies virus or pig parvoviral.
In some embodiments, cell growth temperature is at 35 DEG C~37 DEG C, connect malicious restrovirus production temperature 30 DEG C~
37℃。
In some embodiments, cell growth inoculum density is 0.3 × 106Cell/mL~1.0 × 106Cell/mL, it is raw
Long number of days reached highest cell density at 2~8 days.
In other words, the technical problem that the present invention solves is that overcome the deficiencies of the prior art and provide a kind of adherent ST thin
Low serum, serum-free domestication and the suspension acclimation method of born of the same parents, makes cell growth state stabilization, good dispersion, is suitable for scale
Change culture, and is conducive to the duplication and expression of virus.
For this purpose, the present invention enables the cell strain of original adherent growth by the change of culture medium and the change of training method
Enough low serum or serum-free, suspension high-density growth.Wherein, low serum domestication includes during gradually reducing cell secondary culture
Culture medium serum-concentration, until the condition of culture under the cell adapted low-serum-concentrations of ST.The place of starting for the domestication of low serum
In optimum state and stablize growth ST cells can be from freeze adherent ST cell recoveries, restore ST cells.
Then, after ST cells have adapted to adherent low Serum Growth completely, growth training is defined using serum-free chemical composition
It supports base CD ST 225 (JSB-DP225) and carries out serum-free domestication and the domestication that suspends, until ST cells adapt to the culture side that suspends completely
Formula, and the ST cells that suspend can start stable passage growth, and suspension ST cell densities can reach 8.0 × 106Cell/mL
~20.0 × 106Between cell/mL, motility rate maintains 95% or more.
ST cells in the present invention have that be accredited as American type culture collection (ATCC) number be CRL-1746
Cell line feature, it is commercially available.Suspension ST cells in the present invention are the suspension ST cells for adapting to serum free suspension culture,
Either commercially available suspension ST cells, can also be to be tamed as described above through low serum, serum-free by adherent ST cells
And/or the suspension ST cells of suspend domestication and acquisition.DMEM complete mediums in the present invention are the DMEM trainings containing 10%NBCS
Base finished product is supported,BD004 culture mediums are strong suitable biology commercialization culture medium, and CD ST 225 are strong suitable biology business
The culture medium of change.Compared with prior art, the present invention having the advantage that:The suspension ST cell growth states that the present invention turns out are steady
Determine, dispersibility preferably, adapts to carry out suspension culture in serum-free and the culture medium of chemical composition determination completely, being capable of scale
Production.
The present invention also provides a kind of second order cultural methods of suspension ST cell related vaccines production, and it includes following steps
Suddenly：
1) cell growth：Suspension ST cells grow to 2.0 × 10 in growth medium6Cell/mL~20.0 × 106Carefully
Born of the same parents/mL；
2) poison is connect：According to production technology, reach 6.0 × 10 second day of cell growth or third day, cell density6
Cell/mL~10.0 × 106Cell/mL, selection connect poison amount 10-1~10-5Between MOI, or according to this after step 3)
It connects poison amount and connects poison；
3) dilute/add production medium：1~5 times is diluted with production medium, cell density is to 1.0 × 10 after dilution6
Cell/mL~10.0 × 106Cell/mL；
4) it harvests：Cell diauxic growth simultaneously purifies seedling according to production technology harvest virus liquid..
In some embodiments, harvest virus liquid purifying seedling refers to according to production technology harvest virus liquid purifying system
Seedling, wherein the production technology refers to that the known production technology of corresponding vaccine is produced using ST cells.
In some embodiments, ST cell growths are suspension tank culture, and training volume is 30L or more, such as
30L, 35L, 40L, 50L, 60L, 70L, 80L, 90L, 100L or bigger.
In some embodiments, the growth medium of ST cells is the culture medium of any growth suitable for ST cells,
Preferably, the culture medium is 225 serum free mediums of CD ST.
In some embodiments, in step 1) cell density 4.0 × 106Cell/mL~15.0 × 106Cell/mL,
Preferably, 5.0 × 106Cell/mL~12.0 × 106Cell/mL, most preferably, 10.0 × 106Cell/mL.
In some embodiments, cell connects poison amount 10 in step 2)-1~10-4Between MOI, more excellent ground connection, 10-1~
10-3MOI, optimal ground connection, 10-2MOI。
In some embodiments, ST cells production medium is diluted to 1~4 times in step 3), it is highly preferred that 1~3 times
Dilution, most preferably 2 times dilutions.
In some embodiments, cell density is 1.5 × 10 after poison being connect in step 3)6Cell/mL~4.5 × 106Carefully
Born of the same parents/mL, it is preferable that 2.0 × 106Cell/mL~3.0 × 106Cell/mL.
In some embodiments, the cell density in step 4) after diauxic growth is 2.0 × 106Cell/mL -20.0 ×
106Cell/mL, for example, 2.0 × 106Cell/mL, 3.0 × 106Cell/mL, 4.0 × 106Cell/mL, 5.0 × 106Cell/
mL、6.0×10 6Cell/mL, 7.0 × 106Cell/mL, 8.0 × 106Cell/mL, 9.0 × 106Cell/mL, 10.0 × 106Carefully
Born of the same parents/mL, 11.0 × 106Cell/mL, 12.0 × 106Cell/mL, 13.0 × 106Cell/mL, 14.0 × 106Cell/mL, 15.0
×106Cell/mL, 16.0 × 106Cell/mL, 17.0 × 106Cell/mL, 18.0 × 106Cell/mL, 19.0 × 106Cell/
mL、20.0×106Cell/mL.
In some embodiments, ST cells be production of vaccine used in suspension cell, or by it is adherent domestication from, can
The suspension cell of suspension growth production.
In some embodiments, virus is the disease that is sensitive, can producing corresponding vaccine on corresponding suspension ST cells
Poison.In some embodiments, virus is selected from swine fever virus, Pseudorabies virus, pig parvoviral, transmissible gastroenteritis of swine virus
Or pig circular ring virus.
In some embodiments, cell growth temperature is at 35 DEG C~37 DEG C, connect malicious restrovirus production temperature 30 DEG C~
37℃。
In some embodiments, cell growth inoculum density is 0.3 × 106Cell/mL~1.0 × 106Cell/mL, example
Such as 0.3 × 106Cell/mL, 0.4 × 106Cell/mL, 0.5 × 106Cell/mL, 0.6 × 106Cell/mL, 0.7 × 106Cell/
mL、0.8×106Cell/mL, 0.9 × 106Cell/mL or 1.0 × 106Cell/mL, it is thin that growth number of days at 2~8 days reached highest
Born of the same parents' density, such as 3 days, 4 days, 5 days, 6 days, 7 days or 8 days.
The novelty of the present invention is before cell growth to plateau, and cell density reaches highest, then dilutes original training
Volume is supported, the process of virus is inoculated, can be from low-density diauxic growth by diluted cell, virus also replicates therewith, finally
Improve viral yield.The advantage of the second order culture of the ST cell related vaccines production of the present invention is：Simple production process is easy
Amplification；Liquid is not changed, culture medium utilization rate is high；Production medium and growth medium can be identical culture medium, or
Different culture mediums.Production medium and growth medium proportionally mix, and can not only have the function that cell growth, but also can
To have the function that virus replication is proliferated.
Description of the drawings
Fig. 1 shows ST cells in the third day of growth, and cell density reaches 5.0 × 106When cell/mL, virus inoculation
And adsorb, the production medium of 1 times of original working volume is added, cell density becomes 2.5 × 10 at this time6Cell/mL, cell is again
Start diauxic growth, by growth in 1 day, cell density can reach to 7.2 × 106Cell/mL.
Fig. 2 shows that ST cell growths to third day, are inoculated with Pseudorabies virus restrovirus titre and reach highest in 48h,
TCID50For log10More than 8.5/0.1mL.
Specific implementation mode
The novelty of the present invention is：Adherent growth ST cells using the present invention low serum, serum-free acclimation method and
After the domestication of suspension acclimation method, the domestication cell growth state that is obtained is stable, good dispersion, is suitable for high density, scale
Culture, and be conducive to the duplication and expression of virus.Suspension ST cells reach certain cell density when growing into plateau, such as
8.0 × 106When cell/mL, production medium is added and dilutes original volume of culture, inoculates virus.ST cells are diluted to low
Start diauxic growth after density again, virus also replicates therewith, finally improves viral yield.
In order to reach the above production advantage, present invention firstly provides a kind of changes that adherent ST cell strains pass through culture medium
With the change of training method, the low serum of the cell strain of original adherent growth or serum-free, suspension high-density growth is enable to tame and docile
Change method, the low serum refer in culture medium serum-concentration less than without domestication adherent ST cell strains normal growth, proliferation
When culture medium needed for serum-concentration, for example, low serum refer in culture medium serum-concentration less than 4%, 3%, 2%, 1% or
It is lower, it is preferable that serum-concentration is less than 2%, it is highly preferred that serum-concentration is less than 1%.The serum-free refer in culture medium not
Serum containing any animal origin or its ingredient.A concentration of concentration of volume percent.The low blood of the adherent ST cells
Clear domestication is comprising culture medium serum-concentration during cell secondary culture is gradually reduced, until the cell adapted low-serum-concentrations of ST are such as
Condition of culture under 1% serum-concentration.Specifically, the low serum domestication includes to make in optimum state and stablize growth
ST cells are in low blood serum mediumBD004 (JSB-DP049) plus 2.5~3.5% (such as 3%) newborn bovine serum
(NBCS) passage 1~4 time in, such as 3~4 times make cell be restored to optimum state and stablize growth, then in low blood serum mediumIt is passed on 1~4 time in BD004 (JSB-DP049) plus 2.2~2.4% (such as 2%) newborn bovine serum (NBCS), such as 3
~4 times, so that cell is restored to optimum state and stablize growth, then in low blood serum mediumBD004(JSB-
DP049 it) plus in 0.8~1.5% (such as 1%) newborn bovine serum (NBCS) passes on 1~4 time, such as 3~4 times, until cell restores shape
State, being capable of stability passage and doubling time stabilization.For low serum domestication starting in optimum state and stablize growth
ST cells can be from freeze adherent ST cell recoveries, restore ST cells.Specifically, the recovery, recovery relate to
And after making the adherent ST cell recoveries frozen, in the serum containing normal concentration, such as 10% fetal calf serum or newborn bovine serum, training
Support base (such asDMEM (high glucose)) in culture, passed on when cell reaches 90% converging state, continuous passage
2~4 times, until cell is restored to optimum state and stablizes growth.
Then, after ST cells have adapted to adherent low Serum Growth completely, growth training is defined using serum-free chemical composition
It supports base CD ST 225 (JSB-DP225) and carries out serum-free domestication and the domestication that suspends.Specifically, the serum-free domestication includes
The ST cells of the adherent low Serum Growth of adaptation are made to define 225 (JSB- of growth medium CD ST in serum-free chemical composition
DP225 growth and passage (being passed on when 90% converging state), repeat the step in), until ST cells adapt to the culture that suspends completely
Mode, and the ST cells that suspend can start stable passage growth.Suspension ST cell densities can reach 8.0 × 106Cell/
ML~20.0 × 106Between cell/mL, motility rate maintains 95% or more.Higher inoculum density initially is usually required when domestication,
Such as 0.5 × 106Cell/mL~1.0 × 106Cell/mL, later stage can be according to 0.3 × 106Cell/mL~0.5 × 106Cell/mL
Passage, cell density is 1.0 × 106Cell/mL~2.0 × 106Cell/mL, motility rate carry out secondary culture 90% or more.
In some embodiments, the low serum or the domestication of its serum-free and/or the culture domestication that suspends include following step
Suddenly：
(1) the adherent ST cell recoveries that will be frozen, it is preferable that the adherent ST cells come from ATCC, number CRL-
1746；
(2) the adherent ST cells of recovery are placed in T75 culture bottles, 15mL is addedDMEM (high glucose)
Add 10% newborn bovine serum (NBCS), is put in 37 DEG C, 5%CO2, humidity >=80% incubator in cultivate 3 days, so that cell is reached
90% converging state；
(3) reject culture medium, and 2mL 0.25%Trypsin-EDTA are added, so that ST cell dissociations is detached from culture dish container
Then 5mL is added in surfaceBD004 (JSB-DP049) adds the complete medium of 10% newborn bovine serum to terminate
Pancreatin digests；Herein it should be noted that if using being in optimum state and having stablized the ST cells grown, step
(1) it is dispensed to step (3)；
(4) above-mentioned postdigestive cell is dispelled with pipette, with the cell density of cell counter unit of account volume
And Cell viability, according to 14000 cells/cm2Inoculum density be inoculated in T75 culture bottles again, 15mL is added
BD004 (JSB-DP049) plus 5% newborn bovine serum, are put in 37 DEG C, 5%CO2Incubator in cultivate 3 days, so that cell is reached
90% converging state；
(5) it is passed on, step by step will every three daysNBCS in BD004 (JSB-DP049) culture medium is down to
3%, 2%, 1%, step (2) and (3) is repeated until cell can be with normal growth when NBCS contents are reduced to 1%, i.e. ST is thin
Born of the same parents adapt toBD004 (JSB-DP049) culture medium adds the complete medium of 1%NBCS；By last time culture
Attached cell reject culture medium and be added 0.25%Trypsin-EDTA make ST cell dissociations be detached from culture bottle surface, be then added
5mL~10mLBD004 (JSB-DP049) culture medium adds the complete medium of 1%NBCS to terminate pancreatin digestion,
1000rpm centrifuges 10min, and supernatant is gone to collect ST cells；
(6) growth medium CD ST 225 (JSB-DP225) are defined using serum-free chemical composition and above-mentioned cell is resuspended extremely
125mL suspends in culture shaking flask, dispels cell mass and so that cell is in dispersity, adjustment cell-seeding-density is 0.5 × 106Carefully
Born of the same parents/mL~1.0 × 106Cell/mL, volume of culture 30mL~50mL are put in 37 DEG C, 5%CO2, humidity >=80%, rotating speed be
It is cultivated 3 days in 120rpm shaking tables, cell density is 1.0 × 106Cell/mL~2.0 × 106Cell/mL, motility rate 90% or more,
Carry out secondary culture；
(7) the cell suspension centrifugation of shaking flask culture, goes supernatant to collect ST cells, repeats step (6), every 3 days according to 0.3 ×
106Cell/mL~0.5 × 106Cell/mL passages are primary, observe cell mass and cell deployment conditions, until after cultivating three days
Cell density can reach 2.0 × 106Cell/mL~4.0 × 106Cell/mL, motility rate is 95% or more, and favorable dispersibility
And without compared with large crumb, you can 100 generation of continuous passage, cell most high-density can reach 8.0 × 106Cell/mL~20.0 × 106Carefully
Born of the same parents/mL, the cell adapted serum-free chemical compositions of suspension ST define growth medium CD ST 225 (JSB-DP225), with 15.0 ×
106Cell/mL freezes, using program temperature reduction box in -80 DEG C of refrigerators for 24 hours, then shift cryopreservation tube to liquid nitrogen container in preserve；
ST cells in the present invention have that be accredited as American type culture collection (ATCC) number be CRL-1746
Cell line feature, it is commercially available.ST cells in the present invention are the ST cells for adapting to serum free suspension culture.In the present invention
DMEM complete mediums be the DMEM culture medium finished products containing 10%NBCS, such asDMEM (high glucose),BD004 culture mediums are strong suitable biology commercialization culture medium, and CD ST 225 are strong suitable biology commercialization culture medium.
Compared with prior art, the present invention having the advantage that:Suspension ST cell growth states that the present invention turns out are stablized, dispersibility compared with
It is good, it adapts to carry out suspension culture in serum-free and the culture medium of chemical composition determination completely, it being capable of large-scale production.
In addition, in order to reach the above production advantage, the present invention also provides one kind to produce about suspension ST cell related vaccines
Second order culture process method, detailed process and steps are as follows：
1) cell growth：Suspension ST cells are grown in growth medium such as CD ST 225 (JSB-DP225) culture medium,
Density reaches 2.0 × 106Cell/mL~20.0 × 106Between cell/mL；Preferred cell density is 6.0 × 106Cell/mL~
15.0×106Cell/mL, it is highly preferred that 9.0 × 106Cell/mL~15.0 × 106Cell/mL, most preferably cell density are
10.0×106Cell/mL；
2) poison is connect：According to production technology, cell connects poison amount 10-1~10-5Between MOI, poison amount is preferably connect 10-1~10- 4MOI, the more excellent poison amount that connects is 10-1~10-3MOI, the optimal poison amount that connects is 10-2MOI；
3) fluid infusion：Production medium is added, the fresh production medium of 1~5 times of original growth medium volume is added, it is excellent
Selection of land, 1~4 times, it is highly preferred that 1~3 times, most preferably 2 times, make cell density reach 1.0 × 106Cell/mL~10.0 ×
106Cell/mL, it is preferable that 2.0 × 106Cell/mL~8.0 × 106Cell/mL, it is highly preferred that 3.0 × 106Cell/mL~
6.0×106Cell/mL, most preferably, 5.0 × 106Cell/mL；
4) diauxic growth：Cell continued growth,
In some embodiments, after the cell density of step 4) diauxic growth reaches technological requirement, including according to life
Production. art harvests the step of virus liquid purifying seedling, wherein the production technology refers to producing corresponding vaccine using ST cells
Known production technology.
Cell growth temperature is at 35~37 DEG C wherein in step 1), such as 35 DEG C, 35.5 DEG C, 36 DEG C, 36.5 DEG C, 37 DEG C.
The suspension ST cells are low serum as described above or the suspension ST cells of the domestication of its serum-free and/or the culture domestication that suspends.
Poison amount is wherein connect in step 2) 10-1~10-5Between MOI, specifically by between production technology and virus and cell
Relationship control, virus production temperature between 30~37 DEG C, such as 30 DEG C, 31 DEG C, 32 DEG C, 33 DEG C, 34 DEG C, 35 DEG C, 35.5
℃、36℃、36.5℃、37℃。
1~5 times that productive culture base unit weight is original growth medium is wherein added in step 3).
Wherein, " growth medium " refers to growing into highdensity low serum or serum-free cell from low-density for cell
Culture medium.
" production medium " refers to the virus production culture medium that can meet virus and effectively replicate.
" growth medium " and " production medium " of the present invention can be that any a or a few moneys known in the art can
For the culture medium of suspension ST cell growths and production.
In some embodiments of the present invention, described " growth medium " and " production medium " can be same trainings
Base is supported, such as is all strong 225 cell culture mediums of commercialization CD ST along biological independent research, number JSB-DP225.At this
In other embodiments of invention, " growth medium " is different culture medium, such as " growth with " production medium "
Culture medium " and " production medium " ratio 1:1、1:2、1:3、1:4、1:5.
The present invention relates to the low serum free culture system and free serum culture of ST cells, the virus for production includes swine fever virus
(SFV), all viruses that can be infected on ST cells including Pseudorabies virus (PRV), pig parvoviral (PPV).
Wherein, described " low serum free culture system " refers to the animal blood serum such as new born bovine of the addition 0.5%~3% in incubation
Serum is to meet the training method of cell growth, such as 1% or 1.5%.
" free serum culture " refers to that animal blood serum such as newborn bovine serum is not added in incubation, is only provided by culture medium
Nutritional ingredient meet cell growth training method.
The wherein described production technology is the bioreactor of 30L or more, including stirring type bioreactor and rip current type
Bioreactor, for example, 50L, 100L, 200L, 300L, 400L, 500L, 1000L, 2000L, 3000L, 4000L, 5000L,
The even greater bioreactors of 10000L.
Second order cultural method refers to, after ST cells are according to certain inoculum density inoculating cell, by growth in 2~8 days, carefully
Born of the same parents' density can reach 2.0 × 106Cell/mL~20.0 × 106Between cell/mL, then virus inoculation adsorbs 0.5~2.0
H, preferably 0.6~1.5h, more preferable 0.7~1.2h, more preferable 0.8~1.1h, most preferably 1.0h, then add original working volume
1~5 times of production medium, makes cell density be reduced to 1.0 × 106Cell/mL~10.0 × 106Between cell/mL, pass through 1
~7 days virus productions, harvest virus liquid and seedling.It is of course also possible in 1~5 times of production for adding original working volume
Poison is connect after culture medium again.The technology is suitable for the production of vaccine for man and live vaccine.
Wherein, " inoculum density " typically refers to, and when cell growth to high density, medium nutrient content cannot meet cell
Growth when, then highdensity cell is proportionally diluted to the process of low-density, general inoculum density 0.1~2.0 ×
106Cell/mL.
" plateau " of the present invention refers to the time that cell reaches highest stand density, and " before plateau " refers to cell
Before reaching highest stand density.
" cell density reaches highest " of the present invention refers to that culture medium disclosure satisfy that the highest growth of cell growth is close
Degree.
Obviously, the above according to the present invention is not departing from this hair according to the general knowledge and conventional process of this field
Under the premise of bright basic fundamental thought, the modifications and changes of diversified forms can also be made.
It will be further illustrated the present invention below by following non-limiting embodiments, it is well known to those skilled in the art, not
In the case of spirit of that invention, many modifications can be made to the present invention, such modification also falls into the scope of the present invention.
Following experimental methods are conventional method unless otherwise instructed.Institute is had using cell strain is accredited as U.S. typical case
The feature for the cell line that culture collection (ATCC) number is CRL-1746, it is commercially available.Used medium is strong suitable
The product of biological independent research.Other used experiment materials unless otherwise instructed, can be obtained easily from commercial company.
Embodiment 1ST cell non-serums tame suspension process
1, experiment material
Cell strain：Adherent ST cells, source：ATCC, number CRL-1746,
Culture medium：DMEM (high glucose) (JSB-66001),BD004(JSB-
DP049), serum-free chemical composition defines growth medium CD ST 225 (JSB-DP225), has listed.
2, experimental method
Steps are as follows：
1) the adherent ST cell recoveries that will be frozen, source：ATCC, number CRL-1746；
2) the adherent ST cells of recovery are placed in T75 culture bottles, 15mL is addedDMEM (high glucose) adds
10% newborn bovine serum (NBCS), is put in 37 DEG C, 5%CO2, cultivate 3 days in the incubator of humidity >=80%, cell made to reach
90% converging state；
3) reject culture medium, and 2mL 0.25%Trypsin-EDTA are added, so that ST cell dissociations is detached from culture dish container
Then 5mL is added in surfaceBD004 (JSB-DP049) plus the complete medium of 10% newborn bovine serum terminate pancreas
Enzymic digestion；
4) above-mentioned postdigestive cell is dispelled with pipette, with the cell density of cell counter unit of account volume and
Cell viability, according to 14000 cells/cm2Inoculum density be inoculated in T75 culture bottles again, 15mL is added
BD004 (JSB-DP049) plus 5% newborn bovine serum, are put in 37 DEG C, 5%CO2Incubator in cultivate 3 days, so that cell is reached
90% converging state；
5) by repeated inoculation cell, cell growth, vitellophag, collect cell, inoculating cell the step of, every three days into
Row passage step by step willNBCS in BD004 (JSB-DP049) culture medium is down to 3%, 2%, 1%, drops every time
After low-serum-concentration, by passing on 1~4 time, such as 3~4 times, so that cell is restored to optimum state and stablize growth, repeat to walk
Rapid 2) and 3) cell can be with normal growth when NBCS contents are reduced to 1%, i.e. ST is cell adapted
BD004 (JSB-DP049) culture medium adds the complete medium of 1%NBCS；By the attached cell reject culture of last time culture
Base and be added 0.25%Trypsin-EDTA make ST cell dissociations be detached from culture bottle surface, then be added 5mL~10mLBD004 (JSB-DP049) culture medium add 1%NBCS complete medium terminate pancreatin digestion, 1000rpm from
Heart 10min goes supernatant to collect ST cells；
6) growth medium CD ST 225 (JSB-DP225) are defined using serum-free chemical composition and cell is resuspended to 125mL
In the culture shaking flask that suspends, dispels cell mass and so that cell is in dispersity, adjustment cell-seeding-density is 0.5 × 106Cell/mL
~1.0 × 106Cell/mL, volume of culture 30mL~50mL are put in 37 DEG C, 5%CO2, humidity >=80%, rotating speed shake for 120rpm
It is cultivated 3 days in bed, cell density is 1.0 × 106Cell/mL~2.0 × 106Cell/mL, motility rate are passed on 90% or more
Culture；
7) the cell suspension centrifugation of shaking flask culture, goes supernatant to collect ST cells, repeats step 6), every 3 days according to 0.3 ×
106Cell/mL~0.5 × 106Cell/mL passages are primary, observe cell mass and cell deployment conditions, until after cultivating three days
Cell density can reach 2.0 × 106Cell/mL~4.0 × 106Cell/mL, motility rate 95% or more, and favorable dispersibility and
Without compared with large crumb, you can 100 generation of continuous passage, cell most high-density can reach 8.0 × 106Cell/mL~20.0 × 106 are thin
Born of the same parents/mL.
2 porcine pseudorabies virus of embodiment is tested in the second order culture process of suspension ST cells
1, experiment material
Cell strain：It can suspension growth through strong along the autonomous domestication of biology from the ST cell strains of ATCC.Its acclimation conditions
It can be as described above.
Culture medium：225 serum free mediums of CD ST (strong along biology JSB, JSB-DP225), have listed.
Porcine pseudorabies virus：Bartha-K61 plants, derive from Hua Nong (Zhaoqing) biological industry Institute for Research and Technology.
2, method
Include the following steps
(1) recovery, passage of cell
(1) preparation of culture medium：225 20L of fluid nutrient medium CD ST are prepared to specifications, are kept away for 4 DEG C after aseptic filtration
Light preserves.
(2) recovery of cell：Freeze-stored cell strain is taken out from liquid nitrogen container, is added in 30mL culture mediums after 37 DEG C of thawings,
1000RPM is centrifuged, and abandons supernatant, be resuspended cell in fresh 1 × 225 cell culture mediums of CD ST, be placed in 37 DEG C, 5%CO2
Rotating speed is to be cultivated in 120rpm culture shaking table culture casees.
Passage：Passage 3~4 times are needed before the cell experiment newly recovered, it is primary every passage in 3 days, inoculum density is 1.0 ×
106Cell/mL, continuous passage 3 days, is then tested.
(3) poison is connect：Cell density reaches 5.0 × 10 when cell is passaged to the 3rd day the 4th generation6Cell/mL, plan cell connect
Malicious density is 2.5 × 106Cell/mL is 5.0 × 10 in cell density6It is inoculated with porcine pseudorabies virus when cell/mL, connects malicious amount
MOI is 10-2, 225 cell culture mediums of benefit CD ST to cell density is 2.5 × 10 after adsorbing 1h6Cell/mL.It is thin to continue culture
Born of the same parents are to 7.0 × 106Cell/mL.
(4) poison is surveyed, the TCID of infective dose Test Virus is cultivated according to median tissue (cell)50。
(2) experimental result
Porcine pseudorabies virus uses the virus titer that ST cell second order culture techniques are produced for TCID50lg108.5/
0.1mL (see Fig. 1 and Fig. 2), high 1 titre of the more conventional method of viral yield, is advantageous in that high-density growth, low-density connect
Poison.The virus titer obtained using conventional method is generally TCID50lg107.5/0.1mL.As seen from Figure 1, ST suspension cells exist
3rd day high density (5.0 × 10 in 225 serum free mediums of CD ST6Cell/mL) growth, 1:1 dilution after low-density (2.5 ×
106Cell/mL) infection Pseudorabies virus, cell of the present invention starts diauxic growth again after connecing poison, does not change liquid, culture medium utilization
It is efficient, it is simple for process, easily amplify.Production cost is saved, virus titer is improved.
Claims (10)
1. a kind of second order cultural method of suspension ST cell vaccines production, it includes following steps：
1) cell growth：Suspension ST cells grow to 2.0 × 10 in growth medium6Cell/mL~20.0 × 106Cell/
mL；
2) poison is connect：Poison amount is connect 10-1~10-5Between MOI, or poison amount is connect according to this after step 3) and connect poison；
3) dilute/add production medium：1~5 times is diluted with production medium, cell density is to 1.0 × 10 after dilution6Cell/
ML~10.0 × 106Cell/mL；
4) diauxic growth：Cell diauxic growth；
5) optionally, harvest virus liquid purifies seedling.
2. the second order cultural method of suspension ST cell vaccines production according to claim 1, wherein suspension ST cell growths
For suspension tank culture, training volume is 30L or more.
3. the second order cultural method of suspension ST cell vaccines production according to claim 1 or 2, wherein cell in step 1)
Density is 4.0 × 106Cell/mL~15.0 × 106Cell/mL, it is preferable that 5.0 × 106Cell/mL~12.0 × 106Cell/
ML, most preferably, 10.0 × 106Cell/mL.
4. the second order cultural method of suspension ST cell vaccines production according to any one of claims 1 to 3, wherein step 2)
Middle cell connects poison amount 10-1~10-4Between MOI, more excellent ground connection, 10-1~10-3MOI, optimal ground connection, 10-2MOI。
5. the second order cultural method of suspension ST cell vaccines production according to any one of claims 1 to 4, wherein step 3)
Middle suspension ST cell production mediums are diluted to 1~4 times, it is highly preferred that 1~3 times of dilution, most preferably 2 times dilutions.
6. the second order cultural method of suspension ST cell vaccines production according to any one of claims 1 to 5, wherein step 3)
In connect poison after cell density 1.0 × 106Cell/mL~10.0 × 106Cell/mL, it is preferable that 1.0 × 106Cell/mL~
5.0×106Cell/mL.
7. the second order cultural method of suspension ST cell vaccines production according to any one of claims 1 to 6, wherein the ST that suspends
Cell be production of vaccine used in suspension ST cells, or by adherent ST cells domestication come, be capable of suspension growth produce suspension
ST cells, it is preferable that suspension ST cells be by adherent ST cells tamed through low serum or serum-free domestication from, the serum-free
Domestication, which refers to the ST cells after domestication, can grow in the culture medium existing for serum-free, be proliferated, and/or, it is preferable that suspension ST is thin
Born of the same parents be by adherent ST cells through suspend domestication from.
8. the second order cultural method of suspension ST cell vaccines production according to any one of claims 1 to 7, wherein virus are
The virus sensitive, that corresponding vaccine can be produced on corresponding suspension ST cells, it is preferable that virus is mad selected from swine fever virus, puppet
Dog disease poison or pig parvoviral.
9. according to the second order cultural method that claim 1 to 8 any one of them suspension ST cell vaccines produce, wherein cell is given birth to
Long temperature connects malicious restrovirus and produces temperature at 30 DEG C~37 DEG C at 35 DEG C~37 DEG C.
10. according to the second order cultural method that claim 1 to 9 any one of them suspension ST cell vaccines produce, wherein cell
Inoculum density is grown 0.3 × 106Cell/mL~1.0 × 106Cell/mL, it is close that growth number of days at 2~8 days reached highest cell
Degree.
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| CN110564698A (en) * | 2019-09-29 | 2019-12-13 | 中国农业科学院兰州兽医研究所 | Method for preparing seneca virus by using suspension cell line |
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| US20050118698A1 (en) * | 2001-09-12 | 2005-06-02 | Chiron Behring Gmbh & Co. | Multiplication of viruses in a cell culture |
| CN104436184A (en) * | 2014-11-25 | 2015-03-25 | 成都威尔诺生物科技有限公司 | Cell production method for influenza virus vaccine |
| CN105112352A (en) * | 2015-07-13 | 2015-12-02 | 郑州爱科生物科技有限公司 | ST cell adapting to full-suspension culture, and application thereof, and vaccine virus culturing method |
| CN105950544A (en) * | 2016-05-17 | 2016-09-21 | 西北民族大学 | Domestication method of full suspension culture type Marc-145 cell line |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050118698A1 (en) * | 2001-09-12 | 2005-06-02 | Chiron Behring Gmbh & Co. | Multiplication of viruses in a cell culture |
| CN104436184A (en) * | 2014-11-25 | 2015-03-25 | 成都威尔诺生物科技有限公司 | Cell production method for influenza virus vaccine |
| CN105112352A (en) * | 2015-07-13 | 2015-12-02 | 郑州爱科生物科技有限公司 | ST cell adapting to full-suspension culture, and application thereof, and vaccine virus culturing method |
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