CN108559781A - A method of cultivating high food utilization efficiency pig - Google Patents
A method of cultivating high food utilization efficiency pig Download PDFInfo
- Publication number
- CN108559781A CN108559781A CN201810266615.7A CN201810266615A CN108559781A CN 108559781 A CN108559781 A CN 108559781A CN 201810266615 A CN201810266615 A CN 201810266615A CN 108559781 A CN108559781 A CN 108559781A
- Authority
- CN
- China
- Prior art keywords
- pig
- genotype
- chromosomes
- measured
- international
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/124—Animal traits, i.e. production traits, including athletic performance or the like
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of methods for cultivating high food utilization efficiency pig.Method provided by the invention, it is that 200546552nd deoxyribonucleotide is A or G on international pig genome No. 13 chromosomes of 11.1 version reference sequences for detect pig to be measured, determine that the genotype of pig to be measured is AA or GG, the remaining feed intake of the pig of GG genotype is less than the pig of AA genotype；The GG genotype is the homozygote that the 200546552nd deoxyribonucleotide is G on international pig genome No. 13 chromosomes of 11.1 version reference sequences；The AA genotype is the homozygote that the 200546552nd deoxyribonucleotide is A on international pig genome No. 13 chromosomes of 11.1 version reference sequences.Breeding can be reduced using the method for the present invention to spend, and effectively improved the pannage utilization ratio in actual production, increased economic efficiency.The method of the present invention is easy to operate, accuracy is high, automatic detection can be achieved, and will play a great role in pig breeding.
Description
Technical field
The present invention relates to biotechnology more particularly to a kind of methods for cultivating high food utilization efficiency pig.
Background technology
In pig production, feed input is maximum, accounts for about 70% or so of total aquaculture cost, food utilization efficiency it is small
Promotion just brings declining to a great extent for feeding cost.Therefore, food utilization efficiency is increasingly valued by people, and has become pig
One of important selection target character in breeding.Food utilization efficiency evaluation index includes mainly feed weightening ratio, gain feed ratio
With remaining feed intake (Residual feed intake, RFI).Remaining feed intake be the practical feed intake of livestock and poultry with for maintaining and
The difference for the required prediction feed intake that increases weight, can reflect Difference of Metabolism that animal itself determines by genetic background (Foster W,
Kilpatrick D,Heaney I.Genetic variation in the efficiency of energy
utilization by the fattening pig.Animal Science.1983, 37(3)：387-393.).Therefore, it remains
The smaller food utilization efficiency for representing pig of the value of remaining feed intake is higher.Currently, remaining feed intake has become feed conversion effect
The best representative index of rate.
The remaining feed intake of estimation needs a large amount of average daily gain data, although the update of electronics feeding equipment with
And the continuous development of feed intake measurement system so that feed intake data collection is more convenient, but it is still costly and time-consuming.Therefore, it reflects
It is fixed very necessary with the relevant molecular labeling of remaining feed intake.
Invention content
It is an object of the present invention to provide a kind of methods for cultivating high food utilization efficiency pig.
The method provided by the invention for cultivating high food utilization efficiency pig is the international 11.1 version reference of pig genome of detection
The 200546552nd deoxyribonucleotide is A or G on No. 13 chromosomes of sequence, determine the genotype of pig to be measured be AA also
It is GG, the remaining feed intake of the pig of GG genotype is substantially less than the remaining feed intake of the pig of AA genotype；The AA genotype is
The homozygosis that the 200546552nd deoxyribonucleotide is A on international pig genome No. 13 chromosomes of 11.1 version reference sequences
Body；The GG genotype is the 200546552nd deoxidation core on international pig genome No. 13 chromosomes of 11.1 version reference sequences
Ribotide is the homozygote of G.
200546552nd on international pig genome No. 13 chromosomes of 11.1 version reference sequences of the determination pig to be measured
Position deoxyribonucleotide is that any existing method can be used in the method for A or G, such as sequencing analysis, PCR-SSCP, PCR-
RFLP methods etc..
200546552nd on international pig genome No. 13 chromosomes of 11.1 version reference sequences of the determination pig to be measured
Position deoxyribonucleotide is the method concretely sequencing analysis of A or G.The sequencing analysis includes PCR amplification and to PCR
The step of amplified production is sequenced；Primer pair used in the PCR amplification meets following condition:It is with the genomic DNA of pig
The product that template carries out PCR amplification contains the 200546552nd on international pig genome No. 13 chromosomes of 11.1 version reference sequences
Position deoxyribonucleotide.
Primer pair used in the PCR amplification concretely single strand dna and sequence shown in the sequence of sequence table 1
The primer pair that single strand dna shown in the sequence 2 of table forms.When carrying out PCR amplification using the primer pair, according to be measured
The difference of the genomic DNA of pig, the DNA fragmentation expanded are nucleosides shown in nucleotide sequence or sequence 4 shown in sequence 3
Acid sequence.
It is a further object to provide a kind of reagents for cultivating high food utilization efficiency pig.
Reagent provided by the invention is international pig genome No. 13 chromosomes of 11.1 version reference sequences for detecting pig to be measured
Upper 200546552nd deoxyribonucleotide is the substance of A or G.
In mentioned reagent, the substance is sequence 2 in single strand dna and sequence table shown in sequence in sequence table 1
Shown in single strand dna composition primer pair.
Application of the mentioned reagent in auxiliary cultivates high food utilization efficiency pig is also the scope of protection of the invention；
Or application of the above-mentioned reagent in preparing auxiliary and cultivating high food utilization efficiency pig is also the model that the present invention protects
It encloses.
The pig to be measured concretely duroc.
The 200546552nd deoxyribonucleotide is deposited on international pig genome No. 13 chromosomes of 11.1 version reference sequences
In the difference (A/G) of a deoxyribonucleotide, g.13.200546552A which is named as>G.GG is pure
It is about 0.1125kg/ days low to close genotypic population ratio AA homozygous genotypes group residue feed intake, so the site can be used as pig and remain
Remaining feed intake trait molecular breeding label.
Third object of the present invention is to provide a kind of methods for cultivating high food utilization efficiency pig.
Method provided by the invention, including the pig of selection GG genotype carry out breeding；The GG genotype is international pig base
Because of the homozygote that the 200546552nd deoxyribonucleotide is G on group No. 13 chromosomes of 11.1 version reference sequences.
The experiment proves that g.13.200546552A the present invention is detected using gene order surveying method>G only needs to carry out
PCR reacts, and sequencing can differentiate that the genotype of individual, genotype sentence that type is very accurate, and testing cost is not high, have very high educate
Kind practical application value.Pig residue feed intake character is selected with the method for the present invention, GG genotype pig ratio AA bases can be made
Because type pig average residual feed intake reduces about 0.1125kg/ days, to obtain high food utilization efficiency pig.It is provided using the present invention
Method to pig carry out breeding, can to pig to be selected carry out early screening, effectively alleviate actual production in when selecting excellent boar
Between long problem, reduce breeding and spend, effectively improve the pannage utilization ratio in actual production.The detection method behaviour of the present invention
Work is simple, expense is not high, accuracy is high, and can realize the direct detection of automation.The present invention will be sent out in the breeding work of pig
Wave great function.
Specific implementation mode
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Primer in following embodiment is synthesized by Shanghai Ying Jun Bioisystech Co., Ltd.
Duroc：The pig is purchased from eyas agriculture and animal husbandry Group Plc.
Embodiment 1, auxiliary identification pig residue feed intake character
One, pig is g.13.200546552A>The determination of G polymorphic sites
1, PCR amplification
According to the 200546552nd dezyribonucleoside on international pig genome No. 13 chromosomes of 11.1 version reference sequences
Sour information, design pair of primers are as follows：
U (sense primer)：5 '-TCCCTTGGCACAACATGGAT-3 ' (sequence 1 of sequence table)；
D (downstream primer)：5 '-TTACAAAGAGCAGGTGGAGGC-3 ' (sequence 2 of sequence table).
It is experiment material to select duroc (2).Using the genomic DNA of duroc as template, primer U and D are used
Carry out PCR amplification.
Amplification system is：Genomic DNA 200ng, 10 × PCR amplification buffer solution, 5 μ l, dNTPs 10mM, upstream and downstream are drawn
Each 50ng of object, Taq archaeal dna polymerases 0.75U, Mg2+2.5mmol/L uses ddH2O supplements reaction system to 50 μ l.
PCR reaction conditions are：95 DEG C of denaturation 5min；Then 95 DEG C of denaturation 20s, 62 DEG C of annealing 30s, 72 DEG C of extension 30s,
Totally 35 cycles；Last 72 DEG C of extensions 10min.
PCR product is after Ago-Gel detects in -20 DEG C of preservations.Using the genomic DNA of 2 durocs as template into
The product of row amplification is respectively designated as product 1, product 2.
2, cloning and sequencing and sequence analysis
Respectively by 2 kinds of PCR products Ago-Gel QIAquick Gel Extraction Kit (Tiangeng biochemical technology Co., Ltd) recovery purifying,
After the DNA fragmentation connection carrier pGEM-T (Promega companies) of recycling, connection product is converted into bacillus coli DH 5 alpha competence
Cell (proud (Beijing) Science and Technology Ltd. tomorrow hundred), according to the carboxylic Bian penicillin resistance label screening positive colony on carrier,
Obtain the recombinant plasmid containing recycling segment.Using T7 the and SP6 promoter sequences on the recombinant plasmid vector as primer pair its into
Row nucleotide sequencing (Invitrogen (Shanghai) Trading Co., Ltd.).Sequencing result shows：Product 1 is sequence M1, product 2
For sequence M2；The length of sequence M1 and sequence M2 are 817bp, only exist the difference (A/G) of a deoxyribonucleotide, this
Deoxyribonucleotide is the 200546552nd deoxyribose on international pig genome No. 13 chromosomes of 11.1 version reference sequences
G.13.200546552A the mononucleotide polymorphic is named as by nucleotide>G.The nucleotide sequence of sequence M1 is shown in the sequence of sequence table
(the 200546552nd deoxyribonucleotide is in sequence on international pig genome No. 13 chromosomes of 11.1 version reference sequences for row 3
In 3 it is corresponding be 5 ' ends the 617th A), the nucleotide sequence of sequence M2 is shown in the sequence 4 of sequence table (international pig genome
It is 5 ' ends that the 200546552nd deoxyribonucleotide is corresponding in sequence 4 on 11.1 chromosomes of version reference sequences 13
Hold the 617th G).By the 200546552nd deoxidation core on international pig genome No. 13 chromosomes of 11.1 version reference sequences
Ribotide is that the homozygote genotype of A is named as AA, the on international pig genome No. 13 chromosomes of 11.1 version reference sequences
The homozygote genotype that 200546552 deoxyribonucleotides are G is GG, their heterozygote genotype is AG.
Two, the 200546552nd dezyribonucleoside on international pig genome No. 13 chromosomes of 11.1 version reference sequences
The correlation analysis of sour polymorphic site and pig residue feed intake
To determine g.13.200546552A>Whether G polymorphic sites and pig residue feed intake are related, with 241 durocs
For experiment material, tested as follows：The genomic DNA for extracting every pig carries out PCR amplifications and right with above-mentioned primer U and D
The pcr amplification product of every pig carries out sequencing analysis, determines that the genotype of every pig is GG or AG or AA, the same step of method
One.Pig residue feed intake is determined according to the genotype of pig：The pig residue feed intake of the pig to be measured of GG genotype is less than AA genotype
Pig to be measured.
Using the daily feed intake of U.S.'s Ao Siben automatic feeding systems 241 durocs of measurement and per daily weight, 90
Age in days starts to measure, weight reach 100kg when terminate, and at this moment use Aquila Vet B-ultrasonic instrument for veterinary use measure pig the thickness of backfat and
Eye muscle area.In experiment remaining feed intake (RFI) calculate with reference to forefathers' method (Onteru SK, Gorbach DM, Young JM,
Garrick DJ,Dekkers JCM,Rothschild MF.Whole genome association studies of
residual feed intake and related traits in the pig.PLoS One,2013, 8(6):
E61756.), formula is as follows：
RFI=ADFI- [1 × (OnBW-30)+b2 × (OffBW-100)+b3 × MetamidBW+b4 × ADGA+b5 ×
OffBFA]
In formula：OnBW refers to preliminary survey weight；OffBW refers to knot and surveys weight；MetamidBW refers to intermediate supersession weight, is equal to
[(OnBW+OffBW)/2]×0.75；ADGA represent 90-180 days between average daily gain corrected value；OffBFA refers to test knot
Value when 10-11 ribbed backs fat thickness is corrected to 100kg weight when beam.The 100kg thickness of backfats and the updating formula of 30-100kg ADGA are come
Derived from Canadian genetic improvement plan.
To g.13.200546552A>Association analysis is carried out in the sites G with remaining feed intake (RFI) with least square method, uses
GLM programs in SAS9.2 softwares coordinate following model to carry out least square variance analysis, compare RFI between each genotype
Difference.The model used is：
Y=μ+G+S+B+P+e
Wherein Y is property determination value；μ is group's mean value；G is genotype effects；S is sex-effects；B is to measure batch effect
It answers；P is parity effect；E is random error.
It the results are shown in Table 1.
1 pig mutational site of table is g.13.200546552A>G genotype is associated with remaining feed intake
Note：The different letters of same column subscript indicate significant difference (P<0.05)
As a result such as table 1, show that the remaining feed intake of GG genotype pigs is substantially less than AA genotype pigs (P<0.05), AG bases
Because the remaining feed intake of type pig and GG genotype pig and AA genotype pigs are without significant difference.Illustrate the international pig genome of the present invention
200546552nd deoxyribonucleotide identification of polymorphisms pig residue feed intake on 11.1 chromosomes of version reference sequences 13
Method is consistent with the practical measurement result of pig residue feed intake.
So in actual pig breeding, it is best to obtain the pig of lower remaining feed intake (higher food utilization efficiency)
The pig of GG genotype is selected to carry out breeding.
Sequence table
<110>Institute of Animal Sciences, Chinese Academy of Agricultural Sciences
<120>A method of cultivating high food utilization efficiency pig
<160> 4
<210> 1
<211> 20
<212> DNA
<213>Artificial sequence
<220>
<223>
<400> 1
tcccttggca caacatggat 20
<210> 2
<211> 21
<212> DNA
<213>Artificial sequence
<220>
<223>
<400> 2
ttacaaagag caggtggaggc 21
<210> 3
<211> 817
<212> DNA
<213>Pig is born in the year of pig（Sus scrofa）
<400> 3
ttcatggcag atggtggtca acttgtctgt gggaggaggt gggatcctgc agaggctgag 60
agcagatgct ttgtcttctg tgacttttca cctgtctagg tttttattcc cccagatgag 120
acaatgaatt tccttgaggg ttggaagtat acagtcatac atttcctgta aaatgcacag 180
agaactagag gatcacgcgg gaactctgga acctgagagg acttttggga attaaggatg 240
tagtgtaggc tagaagtgta cttccttcct tggatcttgg tttacttaag cacttagtgt 300
tcttattgtc taccgtatca tagtttaagt gttcagaata tgttaatatg gtatttactg 360
gtcatgagtt aggaagtact tatcttacca aaatggcatt ttaaggacaa gctatcatca 420
tctaacataa tgccaaattg cgatctttcc atttctttgt ttacgacaca ggaaatccgg 480
gatcctctta tgcagtggct ggggaaacac gtggatcctg aaggagttat aacatctagc 540
aagctctccc ttaaattcgt ttcatcctac acatctgagg tggacatcac cccatctgcc 600
atacctgtgg tgactgacac ggcggccttc tcctcagaaa attttaactt tgagatctac 660
cgacagaatt tgcagaccaa gaaacttgga aaaataattc tgtttgccga agtgacatcc 720
accacaatga gtcttctgga cgggtgagtt ctggcccagc aggcttctgt agctgcttgg 780
aggaatccaa gttaggttga ccgttccctt gaaccag 817
<210> 4
<211> 817
<212> DNA
<213>Pig is born in the year of pig（Sus scrofa）
<400> 4
ttcatggcag atggtggtca acttgtctgt gggaggaggt gggatcctgc agaggctgag 60
agcagatgct ttgtcttctg tgacttttca cctgtctagg tttttattcc cccagatgag 120
acaatgaatt tccttgaggg ttggaagtat acagtcatac atttcctgta aaatgcacag 180
agaactagag gatcacgcgg gaactctgga acctgagagg acttttggga attaaggatg 240
tagtgtaggc tagaagtgta cttccttcct tggatcttgg tttacttaag cacttagtgt 300
tcttattgtc taccgtatca tagtttaagt gttcagaata tgttaatatg gtatttactg 360
gtcatgagtt aggaagtact tatcttacca aaatggcatt ttaaggacaa gctatcatca 420
tctaacataa tgccaaattg cgatctttcc atttctttgt ttacgacaca ggaaatccgg 480
gatcctctta tgcagtggct ggggaaacac gtggatcctg aaggagttat aacatctagc 540
aagctctccc ttaaattcgt ttcatcctac acatctgagg tggacatcac cccatctgcc 600
atacctgtgg tggctggcac ggcggccttc tcctcagaaa attttaactt tgagatctac 660
cgacagaatt tgcagaccaa gaaacttgga aaaataattc tgtttgccga agtgacatcc 720
accacaatga gtcttctgga cgggtgagtt ctggcccagc aggcttctgt agctgcttgg 780
aggaatccaa gttaggttga ccgttccctt gaaccag 817
Claims (6)
1. a kind of method for cultivating high food utilization efficiency pig is to detect international 11.1 version of pig genome of pig to be measured with reference to sequence
The 200546552nd deoxyribonucleotide is A or G on No. 13 chromosomes of row, the genotype with determination pig to be measured be AA also
It is AG or GG, remaining feed intake character is determined according to the genotype of the pig to be measured：The residue of the pig to be measured of GG genotype is adopted
Appetite is less than the pig to be measured of AA genotype；The GG genotype is international pig genome No. 13 chromosomes of 11.1 version reference sequences
Upper 200546552nd deoxyribonucleotide is the homozygote of G, and the AA genotype is 11.1 version ginseng of international pig genome
Examine the homozygote that the 200546552nd deoxyribonucleotide is A on No. 13 chromosomes of sequence.
2. according to the method described in claim 1, it is characterized in that：International 11.1 version of pig genome of the detection pig to be measured
It is sequencing analysis that the 200546552nd deoxyribonucleotide, which is A or G, on No. 13 chromosomes of reference sequences；
The sequencing analysis includes PCR amplification and carries out two steps of sequencing to pcr amplification product；Used in the PCR amplification
Primer pair meets following condition:The product that PCR amplification is carried out using the genomic DNA of the pig to be measured as template contains international pig
200546552nd deoxyribonucleotide on genome No. 13 chromosomes of 11.1 version reference sequences.
3. according to the method described in claim 2, it is characterized in that：Primer pair used in the PCR amplification is by sequence table
The primer pair that single strand dna shown in sequence 2 forms in single strand dna and sequence table shown in sequence 1.
4. application of the reagent according to claim 2 or 3 in auxiliary detects pig residue feed intake to be measured.
5. the application of method, reagent in pig breeding described in claim 1-4.
6. a kind of method for cultivating high food utilization efficiency pig, it is characterised in that：Including selecting the pig of GG genotype to carry out breeding；
The GG genotype is the 200546552nd deoxyribose core on international pig genome No. 13 chromosomes of 11.1 version reference sequences
Thuja acid is the homozygote of G.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810266615.7A CN108559781B (en) | 2018-03-28 | 2018-03-28 | Method for breeding pigs with high feed utilization efficiency |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810266615.7A CN108559781B (en) | 2018-03-28 | 2018-03-28 | Method for breeding pigs with high feed utilization efficiency |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108559781A true CN108559781A (en) | 2018-09-21 |
| CN108559781B CN108559781B (en) | 2022-03-29 |
Family
ID=63533181
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810266615.7A Active CN108559781B (en) | 2018-03-28 | 2018-03-28 | Method for breeding pigs with high feed utilization efficiency |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108559781B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110218799A (en) * | 2019-06-06 | 2019-09-10 | 佛山科学技术学院 | The molecular genetic marker of pig residue feed intake character and application |
| CN110295236A (en) * | 2019-06-06 | 2019-10-01 | 佛山科学技术学院 | The SNP molecular genetic marker of pannage conversion ratio |
| CN110358840A (en) * | 2019-06-06 | 2019-10-22 | 佛山科学技术学院 | The SNP molecular genetic marker of TPP2 gene relevant to remaining feed intake |
| CN113584181A (en) * | 2021-07-26 | 2021-11-02 | 温氏食品集团股份有限公司 | SNP molecular marker related to pig residual feed intake and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103937895A (en) * | 2014-04-24 | 2014-07-23 | 中国农业科学院北京畜牧兽医研究所 | Method and special kit for auxiliary detection of pig intramscular fat content |
| CN105219873A (en) * | 2015-11-04 | 2016-01-06 | 中国农业科学院北京畜牧兽医研究所 | A kind of method and primer special identifying pig vertebra data/coherency shape |
| CN105331696A (en) * | 2015-11-04 | 2016-02-17 | 中国农业科学院北京畜牧兽医研究所 | Method and special primer for identifying pig rib number relevant properties |
-
2018
- 2018-03-28 CN CN201810266615.7A patent/CN108559781B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103937895A (en) * | 2014-04-24 | 2014-07-23 | 中国农业科学院北京畜牧兽医研究所 | Method and special kit for auxiliary detection of pig intramscular fat content |
| CN105219873A (en) * | 2015-11-04 | 2016-01-06 | 中国农业科学院北京畜牧兽医研究所 | A kind of method and primer special identifying pig vertebra data/coherency shape |
| CN105331696A (en) * | 2015-11-04 | 2016-02-17 | 中国农业科学院北京畜牧兽医研究所 | Method and special primer for identifying pig rib number relevant properties |
Non-Patent Citations (2)
| Title |
|---|
| DUY NGOC DO ET AL.: "Genome-wide association and systems genetic analyses of residual feed intake, daily feed consumption, backfat and weight gain in pigs", 《BMC GENETICS》 * |
| 蒲蕾等: "杜洛克猪HMGA1基因多态位点与生长、饲料利用性状的关联分析", 《中国畜牧兽医》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110218799A (en) * | 2019-06-06 | 2019-09-10 | 佛山科学技术学院 | The molecular genetic marker of pig residue feed intake character and application |
| CN110295236A (en) * | 2019-06-06 | 2019-10-01 | 佛山科学技术学院 | The SNP molecular genetic marker of pannage conversion ratio |
| CN110358840A (en) * | 2019-06-06 | 2019-10-22 | 佛山科学技术学院 | The SNP molecular genetic marker of TPP2 gene relevant to remaining feed intake |
| CN110218799B (en) * | 2019-06-06 | 2024-03-26 | 佛山科学技术学院 | Molecular genetic marker for pig residual feed intake traits and application thereof |
| CN113584181A (en) * | 2021-07-26 | 2021-11-02 | 温氏食品集团股份有限公司 | SNP molecular marker related to pig residual feed intake and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108559781B (en) | 2022-03-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108315433B (en) | It is a kind of influence Duroc boar intramuscular fat content molecular labeling and application | |
| CN108559781A (en) | A method of cultivating high food utilization efficiency pig | |
| CN105648077B (en) | A kind of molecular marker for affecting daily gain in pigs character and its application | |
| CN108728552B (en) | It is a kind of influence duroc eye muscle area character molecular labeling and application | |
| CN107164463A (en) | It is a kind of to be used for the SNP marker of measure and/or genetic improvement pig growth traits | |
| CN108913779A (en) | A kind of SNP marker and its application influencing daily gain in pigs character | |
| CN112941199A (en) | Method for evaluating pig backfat thickness and eye muscle area and application thereof | |
| CN113699246B (en) | SNP molecular marker affecting pig feed conversion efficiency character and application thereof | |
| CN112899376B (en) | Method for detecting economic traits of Tibetan chicken by FOXO1 gene SNP marker and application thereof | |
| CN114369669A (en) | Molecular marker related to pork quality traits and application thereof | |
| CN108359733B (en) | Method for auxiliary detection of 100kg backfat thickness of pig | |
| CN112126691B (en) | Method, primer, kit and application for auxiliary detection of pig backfat thickness character by using pig chromosome 2 gene polymorphism site | |
| Farhadian et al. | Polymorphisms in the ovine myostatin gene are associated with birth weight but not with weight gain in Iranian Makoei sheep | |
| CN116837112B (en) | SNP molecular marker related to yak growth traits and application thereof | |
| CN112481385B (en) | SNP marker for detecting pig backfat thickness and application thereof | |
| CN101935706B (en) | Method and special primer pair for detecting quality character of pork | |
| CN112251516B (en) | Method for detecting standard weight and day-age of pigs by using chromosome 7 of pigs and kit thereof | |
| CN112695104B (en) | SNP molecular marker for deep character of eye muscle of white pig and application | |
| CN114231639B (en) | Method for identifying or assisting in identifying area of porcine eye muscle and application thereof | |
| CN105331696B (en) | A kind of method and primer special for identifying pig rib data/coherency shape | |
| CN107779517A (en) | A kind of molecular labeling for influenceing Duroc kind pig lean meat percentage character and its application | |
| CN113699247A (en) | SNP molecular marker on pig No. 1 chromosome and related to pig remaining feed intake and application thereof | |
| CN107586854B (en) | It is a kind of influence duroc waistline character molecular labeling and application | |
| CN110117667B (en) | Method for identifying density of pig muscle fibers and primer pair used by method | |
| CN110157816B (en) | Method for identifying diameter of pig muscle fiber and primer pair used by method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |