CN108551999A - A kind of implantation methods promoting polyoses content in stem of noble dendrobium stalk - Google Patents
A kind of implantation methods promoting polyoses content in stem of noble dendrobium stalk Download PDFInfo
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- CN108551999A CN108551999A CN201810610699.1A CN201810610699A CN108551999A CN 108551999 A CN108551999 A CN 108551999A CN 201810610699 A CN201810610699 A CN 201810610699A CN 108551999 A CN108551999 A CN 108551999A
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- stem
- noble dendrobium
- moso bamboo
- implantation methods
- oxidation processes
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/35—Bulbs; Alliums, e.g. onions or leeks
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/23—Wood, e.g. wood chips or sawdust
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Engineering & Computer Science (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Wood Science & Technology (AREA)
- Beans For Foods Or Fodder (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention discloses a kind of implantation methods promoting polyoses content in stem of noble dendrobium stalk, including following operating procedure:(1)It after fresh moso bamboo is cut into bulk, is then placed in baking oven and dries, then the moso bamboo block surface after drying is subjected to oxidation processes;(2)Moso bamboo block by oxidation processes is put into after fermented soya bean have been fermented with aspergillus oryzae processing and is washed in the waste water of fermented soya beans, salted or other wise, after persistently stirring immersion treatment, takes out drying, functionality moso bamboo block is made;(3)After mixing by functional moso bamboo block, waste edible fungus bacteria stick, wood shavings, peat soil, dendrobe cultivation matrix is made, the dendrobe tissue culture transplantation of seedlings after hardening treatment is subjected to cultivation management in dendrobe cultivation matrix.Using method provided by the invention, sufficient nutritional ingredient can be provided for it in the growth course of the stem of noble dendrobium, ensure that the stem of noble dendrobium has higher survival rate and yield, it can be obviously improved the content of polysaccharide in stem of noble dendrobium stalk in particular by the method for the present invention, and then improve the medical value of the stem of noble dendrobium.
Description
Technical field
The invention belongs to dendrobe cultivation technical fields, and in particular to a kind of plantation side promoting polyoses content in stem of noble dendrobium stalk
Method.
Background technology
Dendrobium is the 2nd big category in orchid, and there are about more than 1400 kinds in the whole world.The existing orchid family Dendrobium Sw in China 74
Kind and 2 mutation are sympathized wherein belonging to precious medicinal plant mostly《Xinhua's book on Chinese herbal medicine outline》26 kinds of Dendrobium Sws are included.Medicinal dendrobium
With multiple efficacies such as reinforcing stomach reg fluid, clearing heat and nourishing yin, anti-inflammatory analgetic, voiceless sound improving eyesight and strengthen immunities.Wherein polysaccharide is the stem of noble dendrobium
Main active, the content of polysaccharide is up to 35.74% in Dendrobidium huoshanness, modern pharmacological studies have shown that the water solubility of the stem of noble dendrobium is more
Human immunity can be remarkably reinforced in sugar, there is anti-cancer, anticancer, anti-aging, radiation resistance.Therefore, in order to further promote the stem of noble dendrobium
Economic value, it is desirable to provide it is a kind of promoted stem of noble dendrobium stalk in polyoses content implantation methods.
Invention content
In order to solve the above technical problem, the present invention provides a kind of implantation methods of polyoses content in promotion stem of noble dendrobium stalk.
The present invention is achieved by the following technical solutions.
A kind of implantation methods promoting polyoses content in stem of noble dendrobium stalk, including following operating procedure:
(1)It after fresh moso bamboo is cut into bulk, is then placed in baking oven and dries, then the moso bamboo block surface after drying is carried out
Oxidation processes;
(2)Moso bamboo block by oxidation processes is put into after fermented soya bean have been fermented with aspergillus oryzae processing and is washed in the waste water of fermented soya beans, salted or other wise, it is lasting to stir
After immersion treatment 10-12 hours, drying is taken out, functionality moso bamboo block is made;
(3)By weight, by 55-65 parts of functional moso bamboo blocks, 22-26 parts of waste edible fungus bacteria sticks, 6-10 parts of wood shavings, 26-28
After mixing, dendrobe cultivation matrix is made, by the dendrobe tissue culture transplantation of seedlings after hardening treatment in dendrobe cultivation base in part peat soil
Cultivation management is carried out in matter, wherein waste edible fungus bacteria stick is dissipating into chunky shape using preceding whole.
Specifically, above-mentioned steps(1)In, the length, width and height of moso bamboo block are respectively 4-8mm, 4-8mm, 4-8mm.
Specifically, above-mentioned steps(1)Neutralization procedure(2)In, the temperature of drying and processing is 45-50 DEG C, drying and processing
Time is 60-70min.
Specifically, above-mentioned steps(1)In, oxidation processes are the ozone Oxidation Treatment 40min using a concentration of 100ppm.
Specifically, above-mentioned steps(1)In, oxidation processes be use mass fraction for 0.9% Peracetic acid oxidation processes
30min。
Specifically, above-mentioned steps(2)In, the time that fermented soya bean have been fermented with aspergillus oryzae processing is 40 hours, the inoculation of aspergillus oryzae
Amount is 0.5%.
From the above technical scheme, it can be seen that the beneficial effects of the invention are as follows:
Using method provided by the invention, sufficient nutritional ingredient can be provided for it in the growth course of the stem of noble dendrobium, ensure stone
Dry measure used in former times has higher survival rate and yield, and polysaccharide in stem of noble dendrobium stalk can be obviously improved in particular by the method for the present invention
Content, and then improve the medical value of the stem of noble dendrobium.Wherein, the present invention is first to bamboo block progress oxidation processes, the bamboo block after oxidation,
Its Surface Creation more hydroxyl, carbonyl, carboxyl, sulfonic group isoreactivity group, therefore its moisture absorption, permeability are more excellent
It is different, while spore in the waste water to washing fermented soya beans, salted or other wise and mycelia have more excellent bond strength, and wash the spore in the waste water of fermented soya beans, salted or other wise
Contain a large amount of small protein and enzyme material with mycelia, the small protein in waste water of the stem of noble dendrobium root to washing fermented soya beans, salted or other wise and
The absorption of enzyme material is stronger, and the stem of noble dendrobium may make to accumulate more polysaccharide in vivo.
Specific implementation mode
Following embodiment cannot be used for limiting the scope of the invention for illustrating the present invention.The reality used in embodiment
The condition of applying can be for further adjustments according to the condition of producer, and unaccounted implementation condition is usually conventional laboratory conditions.
Embodiment 1
A kind of implantation methods promoting polyoses content in stem of noble dendrobium stalk, including following operating procedure:
(1)It after fresh moso bamboo is cut into bulk, is then placed in baking oven and dries, then the moso bamboo block surface after drying is carried out
Oxidation processes;
(2)Moso bamboo block by oxidation processes is put into after fermented soya bean have been fermented with aspergillus oryzae processing and is washed in the waste water of fermented soya beans, salted or other wise, it is lasting to stir
After immersion treatment 10 hours, drying is taken out, functionality moso bamboo block is made;
(3)By weight, 55 parts of functional moso bamboo blocks, 22 parts of waste edible fungus bacteria sticks, 6 parts of wood shavings, 26 parts of peat soils are mixed
After uniformly, dendrobe cultivation matrix is made, the dendrobe tissue culture transplantation of seedlings after hardening treatment is cultivated in dendrobe cultivation matrix
Management, wherein waste edible fungus bacteria stick is dissipating into chunky shape using preceding whole.
Specifically, above-mentioned steps(1)In, the length, width and height of moso bamboo block are respectively 4mm, 4mm, 4mm.
Specifically, above-mentioned steps(1)Neutralization procedure(2)In, the temperature of drying and processing is 45 DEG C, the time of drying and processing
For 60min.
Specifically, above-mentioned steps(1)In, oxidation processes are the ozone Oxidation Treatment 40min using a concentration of 100ppm.
Specifically, above-mentioned steps(1)In, oxidation processes be use mass fraction for 0.9% Peracetic acid oxidation processes
30min。
Specifically, above-mentioned steps(2)In, the time that fermented soya bean have been fermented with aspergillus oryzae processing is 40 hours, the inoculation of aspergillus oryzae
Amount is 0.5%.
Embodiment 2
A kind of implantation methods promoting polyoses content in stem of noble dendrobium stalk, including following operating procedure:
(1)It after fresh moso bamboo is cut into bulk, is then placed in baking oven and dries, then the moso bamboo block surface after drying is carried out
Oxidation processes;
(2)Moso bamboo block by oxidation processes is put into after fermented soya bean have been fermented with aspergillus oryzae processing and is washed in the waste water of fermented soya beans, salted or other wise, it is lasting to stir
After immersion treatment 11 hours, drying is taken out, functionality moso bamboo block is made;
(3)By weight, 60 parts of functional moso bamboo blocks, 24 parts of waste edible fungus bacteria sticks, 8 parts of wood shavings, 27 parts of peat soils are mixed
After uniformly, dendrobe cultivation matrix is made, the dendrobe tissue culture transplantation of seedlings after hardening treatment is cultivated in dendrobe cultivation matrix
Management, wherein waste edible fungus bacteria stick is dissipating into chunky shape using preceding whole.
Specifically, above-mentioned steps(1)In, the length, width and height of moso bamboo block are respectively 6mm, 6mm, 6mm.
Specifically, above-mentioned steps(1)Neutralization procedure(2)In, the temperature of drying and processing is 48 DEG C, the time of drying and processing
For 65min.
Specifically, above-mentioned steps(1)In, oxidation processes are the ozone Oxidation Treatment 40min using a concentration of 100ppm.
Specifically, above-mentioned steps(1)In, oxidation processes be use mass fraction for 0.9% Peracetic acid oxidation processes
30min。
Specifically, above-mentioned steps(2)In, the time that fermented soya bean have been fermented with aspergillus oryzae processing is 40 hours, the inoculation of aspergillus oryzae
Amount is 0.5%.
Embodiment 3
A kind of implantation methods promoting polyoses content in stem of noble dendrobium stalk, including following operating procedure:
(1)It after fresh moso bamboo is cut into bulk, is then placed in baking oven and dries, then the moso bamboo block surface after drying is carried out
Oxidation processes;
(2)Moso bamboo block by oxidation processes is put into after fermented soya bean have been fermented with aspergillus oryzae processing and is washed in the waste water of fermented soya beans, salted or other wise, it is lasting to stir
After immersion treatment 12 hours, drying is taken out, functionality moso bamboo block is made;
(3)By weight, 65 parts of functional moso bamboo blocks, 26 parts of waste edible fungus bacteria sticks, 10 parts of wood shavings, 28 parts of peat soils are mixed
After uniformly, dendrobe cultivation matrix is made, the dendrobe tissue culture transplantation of seedlings after hardening treatment is cultivated in dendrobe cultivation matrix
Management, wherein waste edible fungus bacteria stick is dissipating into chunky shape using preceding whole.
Specifically, above-mentioned steps(1)In, the length, width and height of moso bamboo block are respectively 8mm, 8mm, 8mm.
Specifically, above-mentioned steps(1)Neutralization procedure(2)In, the temperature of drying and processing is 50 DEG C, the time of drying and processing
For 70min.
Specifically, above-mentioned steps(1)In, oxidation processes are the ozone Oxidation Treatment 40min using a concentration of 100ppm.
Specifically, above-mentioned steps(1)In, oxidation processes be use mass fraction for 0.9% Peracetic acid oxidation processes
30min。
Specifically, above-mentioned steps(2)In, the time that fermented soya bean have been fermented with aspergillus oryzae processing is 40 hours, the inoculation of aspergillus oryzae
Amount is 0.5%.
Comparative example 1
Step(2)In, the moso bamboo block Jing Guo oxidation processes is put into clear water, immersion treatment is persistently stirred after 10 hours, takes out
Functionality moso bamboo block is made in drying;Remaining operating procedure is identical with embodiment 1.
Cultivation examination is carried out to the Dendrobidium huoshanness of same breed, identical growing way with the method for each embodiment and comparative example respectively
It tests, other than above-mentioned cultivation condition is different, remaining cultivation condition is identical, is obtained suddenly after then testing each group cultivation
Polyoses content in the stem of noble dendrobium stalk of mountain, test method are as follows:3 years raw healthy and strong, normal Dendrobidium huoshanness stalks are taken, 80 DEG C finish,
Drying to constant weight, is ground into powder, and 80 mesh sieve is crossed, according to liquid ratio 20:1, the condition progress of 90 DEG C of Extracting temperature, extraction 6h
Extraction, extracting solution is concentrated into relative density about 1.05,4 times of 75% ethyl alcohol added to be allowed to precipitate, mixing after five minutes, 3000r/min from
Heart 5min abandons supernatant, and residue is washed with several milliliters of petroleum ether solution, and supernatant is abandoned after centrifugation, repeatedly operation 3-4 times, very
Sky is dry, obtains polysaccharide crude.Iron sheet stone solution polyoses content is measured using phend-sulphuric acid.Test result is as shown in table 1:
Polyoses content in 1 Dendrobidium huoshanness stalk of table
Project | Polyoses content, % |
Embodiment 1 | 39.15 |
Comparative example 1 | 34.22 |
Embodiment 2 | 39.86 |
Embodiment 3 | 40.01 |
As shown in Table 1, a kind of implantation methods promoting polyoses content in stem of noble dendrobium stalk provided by the invention, can effectively be promoted
Polyoses content in stem of noble dendrobium stalk improves the medical value of the stem of noble dendrobium.
Certainly, above description is not limitation of the present invention, and the present invention is also not limited to the example above, the art
Those of ordinary skill, the present invention essential scope in, variation, change, addition or the replacement made, should all belong to the present invention
Protection domain.
Claims (6)
1. a kind of implantation methods promoting polyoses content in stem of noble dendrobium stalk, which is characterized in that including following operating procedure:
(1)It after fresh moso bamboo is cut into bulk, is then placed in baking oven and dries, then the moso bamboo block surface after drying is carried out
Oxidation processes;
(2)Moso bamboo block by oxidation processes is put into after fermented soya bean have been fermented with aspergillus oryzae processing and is washed in the waste water of fermented soya beans, salted or other wise, it is lasting to stir
After immersion treatment 10-12 hours, drying is taken out, functionality moso bamboo block is made;
(3)By weight, by 55-65 parts of functional moso bamboo blocks, 22-26 parts of waste edible fungus bacteria sticks, 6-10 parts of wood shavings, 26-28
After mixing, dendrobe cultivation matrix is made, by the dendrobe tissue culture transplantation of seedlings after hardening treatment in dendrobe cultivation base in part peat soil
Cultivation management is carried out in matter, wherein waste edible fungus bacteria stick is dissipating into chunky shape using preceding whole.
2. a kind of implantation methods promoting polyoses content in stem of noble dendrobium stalk according to claim 1, which is characterized in that above-mentioned
Step(1)In, the length, width and height of moso bamboo block are respectively 4-8mm, 4-8mm, 4-8mm.
3. a kind of implantation methods promoting polyoses content in stem of noble dendrobium stalk according to claim 1, which is characterized in that above-mentioned
Step(1)Neutralization procedure(2)In, the temperature of drying and processing is 45-50 DEG C, and the time of drying and processing is 60-70min.
4. a kind of implantation methods promoting polyoses content in stem of noble dendrobium stalk according to claim 1, which is characterized in that above-mentioned
Step(1)In, oxidation processes are the ozone Oxidation Treatment 40min using a concentration of 100ppm.
5. a kind of implantation methods promoting polyoses content in stem of noble dendrobium stalk according to claim 1, which is characterized in that above-mentioned
Step(1)In, oxidation processes be use mass fraction for 0.9% Peracetic acid oxidation processes 30min.
6. a kind of implantation methods promoting polyoses content in stem of noble dendrobium stalk according to claim 1, which is characterized in that above-mentioned
Step(2)In, the time that fermented soya bean have been fermented with aspergillus oryzae processing is 40 hours, and the inoculum concentration of aspergillus oryzae is 0.5%.
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CN103314738A (en) * | 2013-05-24 | 2013-09-25 | 安徽中升生物科技有限公司 | Dendrobium huoshanense greenhouse planting method |
CN104072305A (en) * | 2014-07-18 | 2014-10-01 | 时科生物科技(上海)有限公司 | Special bamboo charcoal bio-organic fertilizer for dendrobe and production method for bamboo charcoal bio-organic fertilizer |
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CN107285930A (en) * | 2017-08-17 | 2017-10-24 | 金寨县仙炉尖霍山石斛种植专业合作社 | A kind of dendrobium candidum plantation special fertilizer and preparation method thereof |
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CN103314738A (en) * | 2013-05-24 | 2013-09-25 | 安徽中升生物科技有限公司 | Dendrobium huoshanense greenhouse planting method |
CN104072305A (en) * | 2014-07-18 | 2014-10-01 | 时科生物科技(上海)有限公司 | Special bamboo charcoal bio-organic fertilizer for dendrobe and production method for bamboo charcoal bio-organic fertilizer |
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CN105248265A (en) * | 2015-11-04 | 2016-01-20 | 富阳市文曲生态农业开发有限公司 | Method of planting dendrobium officinale by using moso bamboo |
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