CN108542923A - A kind of application from the melanin extracted in black fungus in liver-protecting product - Google Patents

A kind of application from the melanin extracted in black fungus in liver-protecting product Download PDF

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Publication number
CN108542923A
CN108542923A CN201810491918.9A CN201810491918A CN108542923A CN 108542923 A CN108542923 A CN 108542923A CN 201810491918 A CN201810491918 A CN 201810491918A CN 108542923 A CN108542923 A CN 108542923A
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liver
melanin
black fungus
protecting product
hepatic
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傅俊生
戚梦
侯若琳
刘鑫
刘城移
刘建兵
林文雄
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Fujian Agriculture and Forestry University
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A61K36/07—Basidiomycota, e.g. Cryptococcus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/357—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having two or more oxygen atoms in the same ring, e.g. crown ethers, guanadrel
    • A61K31/36—Compounds containing methylenedioxyphenyl groups, e.g. sesamin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/20—Antivirals for DNA viruses
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization

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Abstract

The invention discloses a kind of applications from the melanin extracted in black fungus in liver-protecting product, belong to medicine and food technology field.Black fungus fructification is destroyed the protein in black fungus fructification by the present invention by strong acid, melanin is precipitated out by alkaline solution again, it collects precipitation and obtains melanin coarse extraction, it is purified successively by ethyl alcohol, ethyl acetate, acetone again, melanin after purification is obtained, and uses it for liver-protecting product（Including hepatic or food with effect of hepatic protection）In, the weight percent that wherein melanin ingredient accounts for liver-protecting product is 0.1 ~ 100%.The present invention will be used for from the melanin extracted in black fungus in liver-protecting product, and effective liver protection ingredient melanin has positive effect for virus hepatitis, drug induced hepatic injury, alcoholic liver, the prevention of fatty liver or treatment.In addition, present invention process is simple and practicable, and it is at low cost, it is suitable for industrialized production.

Description

A kind of application from the melanin extracted in black fungus in liver-protecting product
Technical field
The invention belongs to medicine and food technology fields, and in particular to a kind of melanin extracted from black fungus is in liver protection Application in product.
Background technology
Melanin (melanin) is the heterogeneous high-molecular compound made of indoles or phenolic compound oxidation polymerization, Generally in black, brown or brown, being widely present in animals and plants and microorganism has extensive biological function, natural black Pigment has effects that nourishing, health, anti-oxidant and anti-aging etc., has caused the great interest of people, but right both at home and abroad The research of melanin caused by edible mushroom is rarely reported.Currently, related with liver due to the influence of environmental pollution and drug etc. Disease be in the trend that constantly rises, heavy type hepatitis, liver fibrosis, hepatic sclerosis etc. occupy prodigious ratio, liver in crowd Disease, which has become, seriously threatens one of primary root of human health.Carbon tetrachloride is the hepatic injury poisonous substance of classics, through liver cell Metabolism generates free radicals, and causes lipid peroxidation, destroys the complete of liver plasma membrane structure and function, cause liver cell damage and Necrosis.
Hepatoprotective agent refers to the medicine for having the effects that improve liver function, promote liver cell regeneration, enhance liver detoxification function Object, for clinical liver disease.China is the popular big country of hepatitis B, and the incidence of fatty liver is also relatively high.Existing treatment hepatitis B, The hepatic of fatty liver includes removing toxic substances class drug, stimulated hepatic cell regeneration class drug, cholagogic class drug, promotes energetic supersession class medicine Object promotes protein to synthesize class drug and Chinese medicine preparation etc..There are different problems for these hepatic either this or thats.As described in There are also adverse reactions for Chinese medicine preparation, although Schisandra chinens P.E therein can be effectively reduced serum alkaline phosphatase Level is still easily bounced after drug withdrawal, and to the effect unobvious of glutamic-oxalacetic transaminease, some patients with chronic liver paddy occur after taking The phenomenon that raising of careless transaminase.Have again is exactly that late result is poor.
Invention content
It is an object of the invention in view of the shortcomings of the prior art, providing a kind of melanin extracted from black fungus in liver protection Application in product.Melanin is extracted from black fungus, uses it for hepatic or food, for virus hepatitis, drug Property hepatic injury, alcoholic liver, fatty liver prevention or treatment have positive effect.
To achieve the above object, the present invention adopts the following technical scheme that：
It is polished into powder after black fungus is dried, destroys the protein in fructification with acid, then precipitate by alkaline solution, it is heavy to collect Shallow lake obtains melanin coarse extraction, then is purified, and obtained melanin is used in liver-protecting product.
Specifically, being polished into powder after black fungus is dried, by gained black fungus powder, water-bath 1 is small at 60 DEG C in hydrochloric acid When, be then centrifuged for taking precipitation, add sodium hydroxide solution ultrasound 1 hour after centrifuging and taking supernatant, supernatant, which is adjusted to pH, is 1.5 in 80 DEG C of water-baths 12 hours, centrifuging and taking precipitation；Precipitation is washed with deionized water 4 times, melanin coarse extraction is obtained；By gained Melanin coarse extraction uses ethyl alcohol, ethyl acetate, acetone, milli-Q water successively again, and it is standby to be positioned over -20 DEG C of preservations after drying precipitation With melanin sterling being obtained, by melanin after purification in liver-protecting product.
The liver-protecting product includes hepatic or food with effect of hepatic protection.
In the liver-protecting product, the melanin ingredient contained therein extracted from black fungus accounts for the weight of liver-protecting product It is 0.1 ~ 100% to measure percentage.
The hepatic, when the melanin ingredient contained therein extracted from black fungus accounts for the weight of hepatic When measuring percentage less than 100%, other compositions are other liver protection ingredients and auxiliary material, other described liver protection ingredients include bifendate, The auxiliary material includes food starch, polyvinylpyrrolidone, sodium carboxymethylcellulose, magnesium stearate, polyethylene glycol.
The dosage form of the hepatic includes tablet, capsule, granule, dripping pill, microcapsules, vina, syrup, mouth Take liquid；When dosage form is microcapsules, effective liver protection ingredient（Melanin）The weight percent for accounting for hepatic is 100%.
The beneficial effects of the present invention are：
The present invention will be used for from the melanin extracted in black fungus in liver-protecting product, and effective liver protection ingredient melanin is for viral Hepatitis, drug induced hepatic injury, alcoholic liver, the prevention of fatty liver or treatment have positive effect.The effect can be by following dynamic Object experiment shows that the experiment has reacted the black fungus melanin of the present invention to carbon tetrachloride（CCl4）Paddy in hepatic injury mice serum The influence of superoxide dismutase and malonaldehyde in pyruvic transaminase and the active influence of glutamic-oxalacetic transaminease and liver homogenate.Experiment Process is as follows：
40 mouse are randomly divided into five groups, every group 10, i.e. positive controls, negative control group, model group, melanin group. Negative control group, sterile water, positive controls 300mg/kg, administration group 100mg/kg.Adaptable fed after a week, using gavage Administration, is administered once a day, successive administration seven days.In after the last administration, after being deprived of food but not water, mouse plucks eyeball and takes blood, centrifugation Afterwards, separation serum is to be measured.After corresponding time cervical dislocation is put to death, about it is put into the same position hepatic tissue of liver lobus sinister rapidly ice-cold Rinsing is wiped dry to no color with filter paper repeatedly in physiological saline, is weighed, with homogenate and homogenizer preparation liver tissue homogenate liquid, Centrifuging and taking supernatant detects indices.
1. influence of the melanin to experimental animal weight
Influence of 1 melanin of table to experiment mice weight
From table 1 it can be seen that during experiment, blank group and administration group do not have significant difference, illustrate from from black fungus The melanin of middle extraction is nontoxic, edible.
2. SOD contents
2 hepatic tissue SOD contents of table
As shown in Table 2, compared with Normal group, SOD activity is substantially reduced in model group murine liver tissue, is had extremely significant Statistical significance shows that damage causes the decline of mouse liver function, modeling success.Positive controls and melanin administration group are equal The vigor of SOD in hepatic tissue can effectively be improved.
3. MDA contents
Malonaldehyde (MDA)：In organism, in lipid peroxidization occurs for Free Radical, and oxidation final product is malonaldehyde, meeting Cause the cross-linked polymeric of the life macromolecules such as protein, nucleic acid, and there is cytotoxicity.MDA is that Lipid peroxidation metabolism is most important One of product, its generation can also aggravate the damage of film therefore MDA contents are in plant senescence physiology and Resistance Physiology research One common counter can understand the degree of Lipid peroxidation metabolism, with the indirect determination membranous system extent of damage by MDA.
3 Liver MDA of table
As shown in Table 3, compared with Normal group, MDA activity is significantly raised in model group murine liver tissue, has extremely significant Statistical significance shows that damage causes the decline of mouse liver function, modeling success.Positive controls and melanin administration group are equal The vigor of MDA in hepatic tissue can effectively be inhibited.
4. ALT contents
ALT (glutamic-pyruvic transaminase）Raising is the important indicator that liver function goes wrong.In common factor, all kinds of livers Inflammation can cause ALT to increase, this is because liver is by caused by destroying.Glutamic-pyruvic transaminase is primarily present in liver cytoplasm Interior, intracellular concentration is higher than in serum 1000-3000 times.As long as there is 1% liver cell to be destroyed, so that it may so that sero-enzyme increases One times.Therefore, glutamic-pyruvic transaminase is the most apparent index of hepatic disorder by world health organisation recommendations.
4 Serum ALT content of table
From table 4：Compared with Normal group, model group mice serum activity is significantly raised, shows that damage causes mouse The decline of liver function, modeling success.Positive controls and melanin group can effectively inhibit the raising of serum active.With model Group compares, and positive controls mice serum activity pole significantly reduces, and positive control medicine is effective.Melanin group mice serum activity Significantly lower than model group, there is significant statistical significance prompt melanin to have significant protection to the damage of caused chmice acute Effect.
5. AST contents
AST（Glutamic-oxalacetic transaminease）It is the index of liver function test on clinical medicine, for judging whether liver receives damage.Liver There are two types of isodynamic enzymes for interior glutamic-oxalacetic transaminease, are respectively present in liver cell mitochondria (mAST) and endochylema (sAST), in liver When lesion that cell is slight, only sAST is released into blood, and mAST can be also discharged into blood in succession when lesion is serious.Therefore serum AST is lived Property increases with the degree of hepatocellular injury
5 serum AST content of table
As shown in Table 5, compared with Normal group, model group mice serum activity is significantly raised, has extremely significant statistics Meaning shows that damage causes the decline of mouse liver function, modeling success.Positive controls and melanin administration group can be effective Inhibition serum active raising.Compared with model group, positive controls mice serum activity pole significantly reduces positive control drug Object is effective.Melanin group mice serum activity is significantly lower than model group, and there is extremely significant statistical significance to prompt melanin pair Caused mouse damage has significant protective effect.
Specific implementation mode
Below in conjunction with specific embodiment, the present invention will be further described, but the present invention is not limited only to these embodiments.
Powder will be polished into after the drying of black fungus fructification, by gained black fungus powder in hydrochloric acid water-bath 1 hour at 60 DEG C, Be then centrifuged for taking precipitation, add sodium hydroxide solution ultrasound 1 hour after centrifuging and taking supernatant, by supernatant be adjusted to pH be 1.5 In 80 DEG C of water-baths 12 hours, centrifuging and taking precipitation；Precipitation is washed with deionized water 4 times, then passes sequentially through ethyl alcohol, ethyl acetate, third Ketone, milli-Q water, drying are positioned over -20 DEG C after precipitating and save backup, and obtain purer melanin.Its pigment content of detection For 2500-3000mg/kg.
Embodiment 1
It will be used in hepatic from the melanin extracted in black fungus, tablet be made；Melanin accounts for the weight hundred of hepatic It is 0.1% to divide ratio, and other compositions are auxiliary material.
Embodiment 2
It will be used in hepatic from the melanin extracted in black fungus, granule be made；Melanin accounts for the weight of hepatic Percentage is 50.0%, and other compositions are auxiliary material.
Embodiment 3
It will be used in hepatic from the melanin extracted in black fungus, capsule be made；Melanin accounts for the weight of hepatic Percentage is 75%, and other compositions are auxiliary material.
Embodiment 4
It will be used in hepatic from the melanin extracted in black fungus, microcapsules be made；Melanin accounts for the weight of hepatic Percentage is 100%.
Embodiment 5
It will be used in hepatic from the melanin extracted in black fungus, dripping pill be made；Melanin accounts for the weight hundred of hepatic It is 5% to divide ratio, and other compositions are auxiliary material.
Embodiment 6
It will be used in hepatic from the melanin extracted in black fungus, vina be made；Melanin accounts for the weight hundred of hepatic It is 25% to divide ratio, and other compositions are auxiliary material.
Embodiment 7
It will be used in hepatic from the melanin extracted in black fungus, oral solution be made；Melanin accounts for the weight of hepatic Percentage is 20%, and other compositions are auxiliary material.
Embodiment 8
It will be used in hepatic from the melanin extracted in black fungus, syrup be made；Melanin accounts for the weight of hepatic Percentage is 30%, and other compositions are auxiliary material.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with Modification should all belong to the covering scope of the present invention.

Claims (6)

1. a kind of application from the melanin extracted in black fungus in liver-protecting product, it is characterised in that：After black fungus is dried It is polished into powder, destroys the protein in fructification with acid, then precipitate by alkaline solution, collection precipitation obtains melanin and slightly carries Object, then purified, obtained melanin is used in liver-protecting product.
2. a kind of application from the melanin extracted in black fungus in liver-protecting product according to claim 1, feature It is：Powder is polished into after black fungus is dried, by gained black fungus powder in hydrochloric acid water-bath 1 hour at 60 DEG C, be then centrifuged for Precipitation is taken, adds sodium hydroxide solution ultrasound centrifuging and taking supernatant after 1 hour, it is 1.5 in 80 DEG C of water that supernatant, which is adjusted to pH, Bath 12 hours, centrifuging and taking precipitation；Precipitation is washed with deionized water 4 times, melanin coarse extraction is obtained；By gained melanin coarse extraction Use ethyl alcohol, ethyl acetate, acetone and milli-Q water successively again, being positioned over -20 DEG C after drying precipitation saves backup, and is purified Melanin afterwards, by melanin after purification in liver-protecting product.
3. a kind of application from the melanin extracted in black fungus in liver-protecting product according to claim 1 or 2, special Sign is：The liver-protecting product includes hepatic or food with effect of hepatic protection.
4. a kind of application from the melanin extracted in black fungus in liver-protecting product according to claim 3, feature It is：In the liver-protecting product, the melanin ingredient contained therein extracted from black fungus accounts for the weight of liver-protecting product Percentage is 0.1 ~ 100%.
5. a kind of application from the melanin extracted in black fungus in liver-protecting product according to claim 3, feature It is：The hepatic, when the melanin ingredient contained therein extracted from black fungus accounts for the weight of hepatic When percentage is less than 100%, other compositions are other liver protection ingredients and auxiliary material, other described liver protection ingredients include bifendate, institute It includes food starch, polyvinylpyrrolidone, sodium carboxymethylcellulose, magnesium stearate, polyethylene glycol to state auxiliary material.
6. a kind of application from the melanin extracted in black fungus in liver-protecting product according to claim 3, feature It is：The dosage form of the hepatic includes tablet, capsule, granule, dripping pill, microcapsules, vina, syrup, takes orally Liquid；When dosage form is microcapsules, the weight percent that effective liver protection ingredient accounts for hepatic is 100%.
CN201810491918.9A 2018-05-22 2018-05-22 A kind of application from the melanin extracted in black fungus in liver-protecting product Pending CN108542923A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109549959A (en) * 2018-11-29 2019-04-02 福建农林大学 A method of black fungus melanin is extracted using cellulase-ultrasonic synergistic

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CN104382953A (en) * 2014-11-28 2015-03-04 哈尔滨墨医生物技术有限公司 Extraction method of melanin in black fungi

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CN104382953A (en) * 2014-11-28 2015-03-04 哈尔滨墨医生物技术有限公司 Extraction method of melanin in black fungi

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109549959A (en) * 2018-11-29 2019-04-02 福建农林大学 A method of black fungus melanin is extracted using cellulase-ultrasonic synergistic
CN109549959B (en) * 2018-11-29 2021-07-23 福建农林大学 Method for extracting black fungus melanin by using cellulase-ultrasonic wave synergism

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