CN108522550A - A kind of preparation method of sterilizing synergistic agent - Google Patents
A kind of preparation method of sterilizing synergistic agent Download PDFInfo
- Publication number
- CN108522550A CN108522550A CN201810404727.4A CN201810404727A CN108522550A CN 108522550 A CN108522550 A CN 108522550A CN 201810404727 A CN201810404727 A CN 201810404727A CN 108522550 A CN108522550 A CN 108522550A
- Authority
- CN
- China
- Prior art keywords
- synergistic agent
- sterilizing
- preparation
- tea saponin
- xanthans
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/10—Apiaceae or Umbelliferae [Carrot family], e.g. parsley, caraway, dill, lovage, fennel or snakebed
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of preparation methods of sterilizing synergistic agent, according to xanthans, Tea Saponin and celery root extract 10:2~3:5~6 mass ratio, xanthans is dissolved completely in alcohol, then Tea Saponin, celery root extract are dissolved in the alcoholic solution of xanthans successively, and stirring is to being completely dissolved up to sterilizing synergistic agent.It has the beneficial effect that：Sterilizing synergistic agent preparation process is simple, from plant, it is cheap, the opening dimension of plant surface stomata can be expanded, to be conducive to the infiltration of liquid, the bactericidal effect of fungicide is greatly improved, while sterilizing synergistic agent also has sterilizing function, the preparation process of synergist active ingredient can greatly improve its purity and yield, enhance the synergistic effect to fungicide.
Description
Technical field
The present invention relates to fungicide fields, more particularly, to a kind of preparation method of sterilizing synergistic agent.
Technical background
Fungicide is also known as biocide, Biocidal algae-killing agent, microbicide etc., typically refers to efficiently control or kill water
The chemicals of microorganism-bacterium, fungi and algae in system.In the world, typically as all kinds of micro- lifes of cause of disease of prevention
The general name of the medicament of object.The whole world to the pathogenic microorganism of plant pest (fungi, strong bacterium, rickettsia, mycoplasma, virus,
Algae etc.) there are 80,000 kinds or more.Plant disease brings about great losses to agricultural, thus global crops are reduced every year on average
Yield about 500Mt.Once repeatedly occurred to cause seriously to be hard up because of certain plant disease epidemics in history, or even a large amount of populations are hungry to death
Calamity.The use of fungicide is a kind of cost-effective method of controlling plant diseases.Organic solvent and auxiliary agent are in Pesticide use
Environment can be fully entered in the process, while causing seriously to pollute environment, can also damage health.As health of people is anticipated
The raising of knowledge reduces persticide residue, makes great efforts the needs that exploitation new type bactericide synergist is the sustainable development of socio-economy.
Invention content
The purpose of the present invention is to provide a kind of preparation methods of sterilizing synergistic agent, and sterilizing synergistic agent preparation process is simple,
It is cheap from plant, the opening dimension of plant surface stomata can be expanded, to be conducive to the infiltration of liquid, significantly
The bactericidal effect of fungicide is improved, while sterilizing synergistic agent also has sterilizing function, the preparation process of synergist active ingredient can
To greatly improve its purity and yield, enhance the synergistic effect to fungicide.
The present invention is directed to the problem of being mentioned in background technology, and the technical solution taken is：
A kind of sterilizing synergistic agent is the mixture of xanthans, Tea Saponin and celery root extract, and specially weight ratio is 10:2~
3:5~6 xanthans, the mixture of Tea Saponin and celery root extract；Sterilizing synergistic agent can join while to fungicide synergy
It is killed with to pathogen, increases substantially the bactericidal effect of fungicide, be a kind of to prepare simple, effective sterilization synergy
Agent.
A kind of sterilizing synergistic agent, preparation method are：Xanthans is dissolved completely in the alcohol of 5~8 times of amounts 50~70%
In, half-light, at a temperature of 1~2 DEG C, then Tea Saponin, celery root extract are dissolved in the alcoholic solution of xanthans successively, stirring is extremely
It is completely dissolved up to sterilizing synergistic agent；Xanthans, Tea Saponin and celery root extract compound sterilizing synergist sterilize substituted benzene
Agent such as thiophanate methyl, Bravo, fenaminosulf, pentachloronitrobenzene etc. and azoles fungicide for example triadimefon, carbendazim, hymexazol,
There is stronger synergy gain effect, xanthans can not only be substantially reduced liquid with alcoholic solution for benomyl, probenazole etc.
Surface tension is conducive to wetting and expansion of the liquid on blade, and the two cooperation can enhance the homogeneity of liquid, keep liquid equal
Even is adhered to plant surface resistance of rainwater washing against, to improve drug effect, extend timeliness；Tea Saponin can expand with celery root extract
The opening dimension of plant surface stomata, to be conducive to the infiltration of liquid, meanwhile, penetrate into the Tea Saponin and celery root of plant tissue
Extract also kills effect to such as Pathogen of Fusarium Wilt generation of part pathogenic bacteria, and the two synergistic effect can infiltrate through cause of disease thalline
It is interior, the genetic transcription of pathogen is obstructed, the generting machanism of acetyl coenzyme A is destroyed, reduces secretion and the activity of acetyl coenzyme A, and
The luminous efficiency of pathogen tricarboxylic acid cycle is further decreased, inhibits pathogen to manufacture energy, to kill pathogen, to killing
It also participates in killing pathogen while microbial inoculum synergy, increases substantially the bactericidal effect of fungicide.
Preferably, the extracting method of Tea Saponin is：Cake of camellia oleifera seeds was crushed into 60~100 mesh sieve, was made using petroleum ether
For extraction solvent, in 55~60 DEG C of Extracting temperature, solid-liquid ratio 1g:6~10h of degreasing under conditions of 8~10mL has been removed residual
The cake of camellia oleifera seeds of oil；Degreasing grouts crushed after being dried is weighed, is placed in container, by 1g:7~8mL solid-liquid ratios, weight ratio, which is added, is
10~11:100 tetrahydrofuran and the mixed liquor of ethanol solution are dissolved with 0.5~0.55 ‰ in advance wherein in tetrahydrofuran
(R)-(+)-methyl p-tolyl sulfoxide, the mass fraction of ethanol solution are 68~70%, flow back 100 under 60~62 DEG C of water-baths
~110min, is filtered while hot, and filtrate is concentrated in vacuo, and acetone precipitation is then added and goes out Tea Saponin, filters and collect precipitation,
It is drying to obtain Tea Saponin；Tea Saponin is extracted using this method, and the yield of Tea Saponin is up to 12.5~15.8%, the ethyl alcohol of low concentration
Leaching liquor concentration can be made very difficult, bubble phenomenon is serious, with gradually increasing for concentration of alcohol, the objects such as protein, pectin
Matter can be accelerated to solidify, and be reduced so as to cause Tea Saponin extraction yield, and the tetrahydrofuran of specific proportioning is added in ethanol solution
The yield of Tea Saponin can be then greatly improved with (R)-(+)-methyl p-tolyl sulfoxide, the two has in the case of specific proportioning
There is gain effect, which can prevent absorption of the aggregates such as denatured protein, pectin to Tea Saponin, enhance ethanol solution pair
The solubility property of Tea Saponin, so as to substantially reduce containing for the Tea Saponin being precipitated with denatured protein, pectin isogel group
Amount, and finally improve the yield and purity of Tea Saponin；Tea Saponin is a kind of nonionic surfactant of function admirable, have compared with
The effects that strong foaming, emulsification, dispersion, moistening, and with physiological activity such as antibacterial, anti-inflammatory, analgesia, anticancers, may be utilized in fabricating
All kinds of detergent, cosmetics, foaming agent, preservative, emulsifier, insecticide and other medicaments.
Preferably, the extracting method of celery root extract is：In 8~10 leaf phase of celery, celery is randomly selected, fresh quench is taken to wash
Only, it is cut into after the segment that length is 0.5~0.1cm according to 1g:The amount of 3~5mL is soaked into absolute ethyl alcohol, and oscillation 18 under room temperature~
For 24 hours, it is then crossed with crocus cloth and filters out residue, then fungi is filtered off up to mother liquor with sterilizing filter；Using absolute ethyl alcohol as eluent
Leaching liquor mother liquor is detached using silica gel column chromatography, it is 100~150 μm that stationary phase, which selects silica gel, silica gel grain size, layer
It analyses and dries chromatographed product and obtain celery root extract；Celery root extract can inhibit the cell wall degradation secreted by Pathogen of Fusarium Wilt
The activity of enzyme, to reduce the ability that pathogen decomposes cell wall, celery root extract can also be broken in Pathogen of Fusarium Wilt
Enzymatic protective system original equilibrium state in portion's makes it reduce the Scavenging activity of active oxygen, Free-radical ring opening polymerization is caused to occur
Obstacle, H2O2A large amount of accumulation, destroy somatic cells film, so that pathogen is damaged and is destroyed, substantially reduce Pathogen of Fusarium Wilt
Pathogenecity.
Advanced optimized as to the present invention, during extracting celery root extract, in absolute ethyl alcohol containing 0.5~
0.8% 2- amino-5-fluorines pyridine and 0.12~0.15 ‰ (-)-sparteine；It is micro during extracting celery root extract
And the 2- amino-5-fluorines pyridine of special proportioning can effectively destroy the cell wall of celery rhizosphere cell with (-)-sparteine, it can
So that the protein of celery rhizosphere cell wall crosslinks degradation, to destroy cell wall, make dissolution of cellular content, greatly
It is big to increase the extraction rate of effective antibacterial substance in content, while extraction time can also be shortened, extraction power consumption is reduced, is improved
The content and purity of effective antibacterial substance, to enhance fungistatic effect and the synergistic effect of synergist.
Compared with the prior art, the advantages of the present invention are as follows：
1) xanthans, Tea Saponin and celery root extract compound sterilizing synergist have substituted beneze-group fungicides and azoles fungicide
Stronger synergy gain effect, xanthans can be substantially reduced the surface tension of liquid with alcoholic solution, be conducive to liquid in blade
On wetting and expansion, to synergy fungicide bactericidal effect；
2) both Tea Saponin and the celery root extract for penetrating into plant tissue are cooperateed with and can be infiltrated through in Pathogen of Fusarium Wilt body, obstruction
The genetic transcription of pathogen destroys the generting machanism of acetyl coenzyme A, reduces secretion and the activity of acetyl coenzyme A, further decreases
The luminous efficiency of pathogen tricarboxylic acid cycle inhibits pathogen to manufacture energy, to kill pathogen；
3) during extracting celery root extract, 2- amino-5-fluorines pyridine and (-)-sparteine of micro and special proportioning can have
The cell wall of the destruction celery rhizosphere cell of effect, can make the protein of celery rhizosphere cell wall crosslink degradation, from
And cell wall is destroyed, make dissolution of cellular content, shorten extraction time, reduce extraction power consumption, improves containing for effective antibacterial substance
Amount and purity, to enhance fungistatic effect and the synergistic effect of synergist.
Specific implementation mode
The present invention program is described further below by embodiment：
Embodiment 1：
A kind of sterilizing synergistic agent is the mixture of xanthans, Tea Saponin and celery root extract, and specially weight ratio is 10:2:5
The mixture of xanthans, Tea Saponin and celery root extract；Sterilizing synergistic agent can participate in while to fungicide synergy to cause of disease
Bacterium is killed, and the bactericidal effect of fungicide is increased substantially, and is a kind of to prepare simple, effective sterilizing synergistic agent.
A kind of sterilizing synergistic agent, preparation method are：Xanthans is dissolved completely in the alcohol of 5 times of amounts 50%, half-light,
At a temperature of 1 DEG C, then Tea Saponin, celery root extract are dissolved in the alcoholic solution of xanthans successively, stirring is to being completely dissolved i.e.
Obtain sterilizing synergistic agent；Xanthans, Tea Saponin and celery root extract compound sterilizing synergist are to substituted beneze-group fungicides such as methyl support
Cloth Tianjin, Bravo, fenaminosulf, pentachloronitrobenzene etc. and azoles fungicide such as triadimefon, carbendazim, hymexazol, benomyl, thiophene bacterium
There is stronger synergy gain effect, xanthans the surface tension of liquid, profit can be not only substantially reduced with alcoholic solution for spirits etc.
In wetting and expansion of the liquid on blade, and the two cooperation can enhance the homogeneity of liquid, and liquid is made uniformly to be adhered to
Plant surface resistance of rainwater washing against, to improve drug effect, extend timeliness；Tea Saponin can expand plant surface gas with celery root extract
The opening dimension in hole, to be conducive to the infiltration of liquid, meanwhile, the Tea Saponin of plant tissue is penetrated into celery root extract also to portion
Pathogenic bacteria such as Pathogen of Fusarium Wilt generation is divided to kill effect, the two synergistic effect can infiltrate through in pathogen body, obstruct cause of disease
The genetic transcription of bacterium destroys the generting machanism of acetyl coenzyme A, reduces secretion and the activity of acetyl coenzyme A, and further decreases disease
The luminous efficiency of opportunistic pathogen tricarboxylic acid cycle inhibits pathogen to manufacture energy, to kill pathogen, to the same of fungicide synergy
When also participate in killing pathogen, increase substantially the bactericidal effect of fungicide.
Embodiment 2：
A kind of sterilizing synergistic agent, preparation method are：
1) Tea Saponin is extracted：Cake of camellia oleifera seeds was crushed into 60 mesh sieve, using petroleum ether as extraction solvent, in Extracting temperature 55
DEG C, solid-liquid ratio 1g:Degreasing 6h under conditions of 8mL has been removed the cake of camellia oleifera seeds of Residual oil；Weigh powder after degreasing grouts are dried
It is broken, it is placed in container, by 1g:7mL solid-liquid ratios, it is 1 that weight ratio, which is added,:10 tetrahydrofuran and the mixed liquor of ethanol solution,
0.5 ‰ (R)-(+)-methyl p-tolyl sulfoxide is dissolved in middle tetrahydrofuran in advance, the mass fraction of ethanol solution is
68%, flow back 100min under 60 DEG C of water-baths, filters while hot, filtrate is concentrated in vacuo, and acetone precipitation is then added and goes out tea
Saponin filters and collects precipitation, is drying to obtain Tea Saponin；Extract Tea Saponin using this method, the yield of Tea Saponin up to 15%,
The ethyl alcohol of low concentration can make leaching liquor concentration very difficult, and bubble phenomenon is serious, with gradually increasing for concentration of alcohol, albumen
The substances such as matter, pectin can be accelerated to solidify, and be reduced so as to cause Tea Saponin extraction yield, and specific proportioning is added in ethanol solution
Tetrahydrofuran and (R)-(+)-methyl p-tolyl sulfoxide can then greatly improve the yield of Tea Saponin, the two is in specific proportioning
In the case of there is gain effect, which can prevent absorption of the aggregates such as denatured protein, pectin to Tea Saponin, enhance
The solubility property of Tea Saponin is precipitated so as to substantially reduce with denatured protein, pectin isogel group for ethanol solution
The content of Tea Saponin, and finally improve the yield and purity of Tea Saponin；Tea Saponin is a kind of non-ionic surface work of function admirable
Property agent, have the effects that stronger foaming, emulsification, dispersion, moistening, and with the physiological activity such as antibacterial, anti-inflammatory, analgesia, anticancer,
It may be utilized in fabricating all kinds of detergent, cosmetics, foaming agent, preservative, emulsifier, insecticide and other medicaments；
2) celery root extract is extracted：In the 8 leaf phase of celery, celery is randomly selected, fresh quench is taken to clean, is cut into the segment that length is 1cm
Afterwards according to 1g:The amount of 5mL is soaked into absolute ethyl alcohol, is vibrated under room temperature for 24 hours, is then crossed with crocus cloth and filters out residue, then with sterile
Filter filters off fungi up to mother liquor；Leaching liquor mother liquor is divided using silica gel column chromatography using absolute ethyl alcohol as eluent
From it is 150 μm that stationary phase, which selects silica gel, silica gel grain size, chromatographs and dry chromatographed product obtains celery root extract；Celery root extract can
To inhibit the activity of the cell wall degrading enzyme secreted by Pathogen of Fusarium Wilt, to reduce the energy that pathogen decomposes cell wall
Power, celery root extract can also break the original equilibrium state of Pathogen of Fusarium Wilt internal protection enzyme system, make it to active oxygen
Scavenging activity reduce, cause Free-radical ring opening polymerization obstacle, H occur2O2A large amount of accumulation, destroy somatic cells film, make cause of disease
Body is damaged and destroys, and substantially reduces the pathogenecity of Pathogen of Fusarium Wilt.
3) it compounds：Xanthans is dissolved completely in the alcohol of 5 times of amounts 70%, half-light, at a temperature of 2 DEG C, then successively by tea
Saponin, celery root extract are dissolved in the alcoholic solution of xanthans, and the weight ratio of xanthans, Tea Saponin and celery root extract is
10:3:6, stirring is to being completely dissolved up to sterilizing synergistic agent；Xanthans, Tea Saponin and celery root extract compound sterilizing synergist pair
Substituted beneze-group fungicides such as thiophanate methyl, Bravo, fenaminosulf, pentachloronitrobenzene etc. and azoles fungicide such as triadimefon, more
Bacterium spirit, hymexazol, benomyl, probenazole etc. have stronger synergy gain effect, and xanthans not only can be bright with alcoholic solution
The aobvious surface tension for reducing liquid is conducive to wetting and expansion of the liquid on blade, and the two cooperation can enhance the equal of liquid
One property, makes liquid uniformly be adhered to plant surface resistance of rainwater washing against, to improve drug effect, extend timeliness；Tea Saponin and celery
Root extract can expand the opening dimension of plant surface stomata, to be conducive to the infiltration of liquid, meanwhile, penetrate into plant tissue
Tea Saponin also kills effect with celery root extract to such as Pathogen of Fusarium Wilt generation of part pathogenic bacteria, and the two synergistic effect can ooze
It penetrates in pathogen body, obstructs the genetic transcription of pathogen, destroy the generting machanism of acetyl coenzyme A, reduce point of acetyl coenzyme A
It secretes and active, and further decreases the luminous efficiency of pathogen tricarboxylic acid cycle, inhibit pathogen to manufacture energy, to kill disease
Opportunistic pathogen also participates in killing pathogen, increases substantially the bactericidal effect of fungicide while to fungicide synergy.
Embodiment 3：
A kind of sterilizing synergistic agent is the mixture of xanthans, Tea Saponin and celery root extract, and specially weight ratio is 10:3:5
The mixture of xanthans, Tea Saponin and celery root extract；Sterilizing synergistic agent can participate in while to fungicide synergy to cause of disease
Bacterium is killed, and the bactericidal effect of fungicide is increased substantially, and is a kind of to prepare simple, effective sterilizing synergistic agent.
A kind of sterilizing synergistic agent, preparation method are：Xanthans is dissolved completely in the alcohol of 6 times of amounts 65%, half-light,
At a temperature of 1 DEG C, then Tea Saponin, celery root extract are dissolved in the alcoholic solution of xanthans successively, stirring is to being completely dissolved i.e.
Obtain sterilizing synergistic agent；Xanthans, Tea Saponin and celery root extract compound sterilizing synergist are to substituted beneze-group fungicides such as methyl support
Cloth Tianjin, Bravo, fenaminosulf, pentachloronitrobenzene etc. and azoles fungicide such as triadimefon, carbendazim, hymexazol, benomyl, thiophene bacterium
There is stronger synergy gain effect, xanthans the surface tension of liquid, profit can be not only substantially reduced with alcoholic solution for spirits etc.
In wetting and expansion of the liquid on blade, and the two cooperation can enhance the homogeneity of liquid, and liquid is made uniformly to be adhered to
Plant surface resistance of rainwater washing against, to improve drug effect, extend timeliness；Tea Saponin can expand plant surface gas with celery root extract
The opening dimension in hole, to be conducive to the infiltration of liquid, meanwhile, the Tea Saponin of plant tissue is penetrated into celery root extract also to portion
Pathogenic bacteria such as Pathogen of Fusarium Wilt generation is divided to kill effect, the two synergistic effect can infiltrate through in pathogen body, obstruct cause of disease
The genetic transcription of bacterium destroys the generting machanism of acetyl coenzyme A, reduces secretion and the activity of acetyl coenzyme A, and further decreases disease
The luminous efficiency of opportunistic pathogen tricarboxylic acid cycle inhibits pathogen to manufacture energy, to kill pathogen, to the same of fungicide synergy
When also participate in killing pathogen, increase substantially the bactericidal effect of fungicide.
The extracting method of Tea Saponin is in the present embodiment：Cake of camellia oleifera seeds was crushed into 80 mesh sieve, using petroleum ether as leaching
Extraction solvent, in 58 DEG C of Extracting temperature, solid-liquid ratio 1g:Degreasing 8h under conditions of 9mL has been removed the cake of camellia oleifera seeds of Residual oil；Claim
Extracting degreasing grouts crushed after being dried, is placed in container, by 1g:7mL solid-liquid ratios, it is 10.5 that weight ratio, which is added,:100 tetrahydrofuran
With the mixed liquor of ethanol solution, 0.52 ‰ (R)-(+)-methyl p-tolyl sulfoxide is dissolved with wherein in tetrahydrofuran in advance,
The mass fraction of ethanol solution is 68%, and flow back 100min under 60 DEG C of water-baths, filters while hot, filtrate is concentrated in vacuo,
Then acetone precipitation is added and goes out Tea Saponin, filters and collects precipitation, be drying to obtain Tea Saponin；Tea Saponin is extracted using this method,
The yield of Tea Saponin is up to 15.5%, and the ethyl alcohol of low concentration can make leaching liquor concentration very difficult, and bubble phenomenon is serious, with
Concentration of alcohol gradually increases, and the substances such as protein, pectin can be accelerated to solidify, and is reduced so as to cause Tea Saponin extraction yield, and
The tetrahydrofuran and (R)-(+)-methyl p-tolyl sulfoxide that specific proportioning is added in ethanol solution can then greatly improve tea
There is gain effect, the effect can prevent denatured protein, pectin etc. in the case of specific proportioning for the yield of saponin, the two
Absorption of the aggregate to Tea Saponin enhances solubility property of the ethanol solution to Tea Saponin, so as to substantially reduce with denaturation egg
White matter, pectin isogel are rolled into a ball and the content of the Tea Saponin of precipitation, and finally improves the yield and purity of Tea Saponin；Tea Saponin is one
The nonionic surfactant of kind of function admirable has the effects that stronger foaming, emulsification, dispersion, moistening, and have antibacterial,
The physiological activity such as anti-inflammatory, analgesia, anticancer may be utilized in fabricating all kinds of detergent, cosmetics, foaming agent, preservative, emulsifier, kill
Worm agent and other medicaments.
The extracting method of celery root extract is in the present embodiment：In the 10 leaf phase of celery, celery is randomly selected, fresh quench is taken to wash
Only, it is cut into after the segment that length is 0.8cm according to 1g:The amount of 4mL is soaked into absolute ethyl alcohol, 20h is vibrated under room temperature, then with thin
Filtered through gauze removes residue, then filters off fungi with sterilizing filter up to mother liquor；Silica gel column layer is utilized by eluent of absolute ethyl alcohol
Analysis method detaches leaching liquor mother liquor, and it is 120 μm that stationary phase, which selects silica gel, silica gel grain size, chromatographs and dry chromatographed product obtains
Celery root extract；Celery root extract can inhibit the activity of the cell wall degrading enzyme secreted by Pathogen of Fusarium Wilt, to reduce
Pathogen decomposes the ability of cell wall, and that celery root extract can also break Pathogen of Fusarium Wilt internal protection enzyme system is original
Equilibrium state makes it reduce the Scavenging activity of active oxygen, Free-radical ring opening polymerization is caused obstacle, H occur2O2A large amount of accumulation, break
It is broken somatic cells film, pathogen is made to be damaged and destroy, substantially reduces the pathogenecity of Pathogen of Fusarium Wilt.
The present embodiment extract celery root extract during, in absolute ethyl alcohol containing 0.6% 2- amino-5-fluorines pyridine with
0.12 ‰ (-)-sparteine；Extract celery root extract during, the 2- amino-5-fluorines pyridine of micro and special proportioning with
(-)-sparteine can effectively destroy the cell wall of celery rhizosphere cell, can make the protein of celery rhizosphere cell wall
Degradation is crosslinked, to destroy cell wall, makes dissolution of cellular content, greatly increases carrying for effective antibacterial substance in content
Rate is taken, while extraction time can also be shortened, extraction power consumption is reduced, improves the content and purity of effective antibacterial substance, to
Enhance fungistatic effect and the synergistic effect of synergist.
Routine operation in operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme of the present invention is described in detail in embodiment described above, it should be understood that the above is only
For specific embodiments of the present invention, it is not intended to restrict the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should all be included in the protection scope of the present invention.
Claims (8)
1. a kind of preparation method of sterilizing synergistic agent, it is characterised in that：The preparation method is that：Xanthans is dissolved completely in wine
In essence, half-light, at a temperature of 1~2 DEG C, then Tea Saponin, celery root extract are dissolved in the alcoholic solution of xanthans successively, it stirs
To being completely dissolved up to sterilizing synergistic agent.
2. a kind of preparation method of sterilizing synergistic agent according to claim 1, it is characterised in that：The weight of the alcohol is
The mass fraction of 5~8 times of xanthans, alcohol is 50~70%.
3. a kind of preparation method of sterilizing synergistic agent according to claim 1, it is characterised in that：The xanthans, tea soap
Element and the mass ratio of celery root extract are 10:2~3:5~6.
4. a kind of preparation method of sterilizing synergistic agent according to claim 1 or 3, it is characterised in that：The Tea Saponin
Extracting method is：Cake of camellia oleifera seeds is crushed, degreasing grouts are obtained using petroleum ether as extraction solvent degreasing；Weigh degreasing grouts
Crushed after being dried is placed in container, and tetrahydrofuran is added and flows back with ethanol solution, filters to take filtrate concentration, acetone is then added
Precipitation is filtered and collected, Tea Saponin is drying to obtain.
5. a kind of preparation method of sterilizing synergistic agent according to claim 4, it is characterised in that：The extraction of the Tea Saponin
In method, the weight ratio of tetrahydrofuran and ethanol solution is 10~11:100,0.5~0.55 ‰ are dissolved in tetrahydrofuran in advance
(R)-(+)-methyl p-tolyl sulfoxide, the mass fraction of ethanol solution is 68~70%.
6. a kind of preparation method of sterilizing synergistic agent according to claim 1 or 3, it is characterised in that：The celery root carries
The extracting method of object is：Choose celery fresh quench, absolute ethyl alcohol be soaked into after being cut into segment, crossed with crocus cloth after oscillation filter out it is residual
Slag, then fungi is filtered off up to mother liquor with sterilizing filter；Utilize silica gel column chromatography to leaching liquor by eluent of absolute ethyl alcohol
Mother liquor is detached, and is chromatographed and is dried chromatographed product and obtains celery root extract.
7. a kind of preparation method of sterilizing synergistic agent according to claim 6, it is characterised in that：The celery root extract
In extracting method, the stationary phase of column chromatography is silica gel, and silica gel grain size is 100~150 μm.
8. a kind of preparation method of sterilizing synergistic agent according to claim 6, it is characterised in that：The extraction celery root carries
During object, (-)-broom top containing 0.5~0.8% 2- amino-5-fluorines pyridine and 0.12~0.15 ‰ in absolute ethyl alcohol
Alkali.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810404727.4A CN108522550A (en) | 2018-04-28 | 2018-04-28 | A kind of preparation method of sterilizing synergistic agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810404727.4A CN108522550A (en) | 2018-04-28 | 2018-04-28 | A kind of preparation method of sterilizing synergistic agent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108522550A true CN108522550A (en) | 2018-09-14 |
Family
ID=63475726
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810404727.4A Pending CN108522550A (en) | 2018-04-28 | 2018-04-28 | A kind of preparation method of sterilizing synergistic agent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108522550A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112450229A (en) * | 2020-12-09 | 2021-03-09 | 华南农业大学 | Plant essential oil type ultraviolet sterilization synergist and ultraviolet combined sterilization method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101589714A (en) * | 2009-06-18 | 2009-12-02 | 浙江省农业科学院 | A kind of pesticide synergistic agent and preparation method thereof |
| CN101817853A (en) * | 2009-03-14 | 2010-09-01 | 兰州理工大学 | Method for extracting tea saponin by adopting tea seed cake |
| CN102925292A (en) * | 2012-10-19 | 2013-02-13 | 伊国群 | Method for comprehensively extracting tea oil, flavonoids compounds, tea saponin and tea polysaccharide |
| CN104262426A (en) * | 2014-08-29 | 2015-01-07 | 王晓伟 | Method for extracting tea saponin |
-
2018
- 2018-04-28 CN CN201810404727.4A patent/CN108522550A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101817853A (en) * | 2009-03-14 | 2010-09-01 | 兰州理工大学 | Method for extracting tea saponin by adopting tea seed cake |
| CN101589714A (en) * | 2009-06-18 | 2009-12-02 | 浙江省农业科学院 | A kind of pesticide synergistic agent and preparation method thereof |
| CN102925292A (en) * | 2012-10-19 | 2013-02-13 | 伊国群 | Method for comprehensively extracting tea oil, flavonoids compounds, tea saponin and tea polysaccharide |
| CN104262426A (en) * | 2014-08-29 | 2015-01-07 | 王晓伟 | Method for extracting tea saponin |
Non-Patent Citations (2)
| Title |
|---|
| 包妍妍: "西芹根物质对黄瓜枯萎病菌化感作用、机理及田间化感作用的研究", 《中国博士学位论文全文数据库 农业科技辑》 * |
| 潘汝谦 等: "黄原胶助剂对杀菌剂田安的增效作用试验", 《农药》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112450229A (en) * | 2020-12-09 | 2021-03-09 | 华南农业大学 | Plant essential oil type ultraviolet sterilization synergist and ultraviolet combined sterilization method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101623013B (en) | Botanical fungicide, extracting method and usage thereof | |
| KR20120100970A (en) | Anthroquinone containing derivatives as biochemical agricultural products | |
| CN106689145A (en) | Weeding composition containing pyroxasulfone and amide herbicides | |
| CN103262847B (en) | Biogenic pesticide containing xanthoxylin and abamectin and preparation method thereof | |
| CN103609572B (en) | A kind of bactericidal composition containing tetramycin and amino-oligosaccharide | |
| CN106332921B (en) | A kind of plant insecticide and its preparation method and application | |
| CN105076218B (en) | A kind of bactericidal composition | |
| CN106665658A (en) | Preparation method for microencapsulated biopesticide | |
| CN108522550A (en) | A kind of preparation method of sterilizing synergistic agent | |
| CN105284828A (en) | Copper abietate and zinc thiazole-containing bactericidal composition | |
| CN1314327C (en) | Use of walnut plant extract as pesticide in preventing and controlling diamondback moth | |
| CN108566958A (en) | A kind of complex biological disinfectant and preparation method thereof | |
| CN105010367A (en) | Sterilization composition containing copper quinolate and zhongshengmycin | |
| CN101589720A (en) | A kind of insecticides that contains indenes worm prestige | |
| CN103719130A (en) | Corn field efficacy enhancement weed killing combination containing thiencarbazone-methyl, mesotrione and atrazine | |
| CN105340915A (en) | Sterilization composition containing benziothiazolinone and Xinjunan acetate | |
| CN105010361A (en) | Sterilization composition containing tetramycin and boscalid | |
| CN101427682A (en) | Bactericidal composition of phenol cycloheximide and dimethomorph | |
| CN101558757A (en) | Insecticidal and acaricidal composition containing spirodiclofen and nitenpyram | |
| CN104970021A (en) | Sterilization composition containing oxine-copper and xinjunan acetate | |
| CN106561697A (en) | Ampullaria gigas molluscacide | |
| CN104488898B (en) | Containing methylsulfonyl bacterium azoles and the complex composition of Xinjunan acetate and antibacterial | |
| CN105340894A (en) | Sterilization composition containing dithianon and bromothalonil | |
| CN105028440A (en) | Sterilization composition containing N,N-dioctyl-diethylenetriamine and zhongshengmycin | |
| CN105145636B (en) | A kind of doractin and biogenic substances complex microemulsion and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180914 |
|
| RJ01 | Rejection of invention patent application after publication |