CN108522247A - A kind of bioreactor of culture ginseng adventitious root - Google Patents
A kind of bioreactor of culture ginseng adventitious root Download PDFInfo
- Publication number
- CN108522247A CN108522247A CN201810398406.8A CN201810398406A CN108522247A CN 108522247 A CN108522247 A CN 108522247A CN 201810398406 A CN201810398406 A CN 201810398406A CN 108522247 A CN108522247 A CN 108522247A
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- Prior art keywords
- tank body
- bioreactor
- fixing piece
- tank
- reactor
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- 241000208340 Araliaceae Species 0.000 title abstract description 20
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 title abstract description 20
- 235000003140 Panax quinquefolius Nutrition 0.000 title abstract description 20
- 235000008434 ginseng Nutrition 0.000 title abstract description 20
- 229910052751 metal Inorganic materials 0.000 claims abstract description 15
- 239000002184 metal Substances 0.000 claims abstract description 15
- 238000005245 sintering Methods 0.000 claims abstract description 9
- 238000003756 stirring Methods 0.000 claims description 20
- 238000010008 shearing Methods 0.000 claims description 9
- 238000009833 condensation Methods 0.000 claims description 6
- 230000005494 condensation Effects 0.000 claims description 6
- 239000007769 metal material Substances 0.000 claims description 6
- 238000011081 inoculation Methods 0.000 claims description 5
- 238000012546 transfer Methods 0.000 claims description 5
- 238000000034 method Methods 0.000 abstract description 11
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 238000012797 qualification Methods 0.000 abstract description 4
- 238000009395 breeding Methods 0.000 abstract description 3
- 230000001488 breeding effect Effects 0.000 abstract description 3
- 238000000855 fermentation Methods 0.000 abstract description 2
- 230000004151 fermentation Effects 0.000 abstract description 2
- 239000010410 layer Substances 0.000 description 15
- 239000007789 gas Substances 0.000 description 12
- 238000001914 filtration Methods 0.000 description 10
- 230000000694 effects Effects 0.000 description 7
- 238000012258 culturing Methods 0.000 description 5
- 238000005520 cutting process Methods 0.000 description 4
- 238000010899 nucleation Methods 0.000 description 4
- 238000003466 welding Methods 0.000 description 4
- 229910001315 Tool steel Inorganic materials 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 229910000838 Al alloy Inorganic materials 0.000 description 1
- 238000011001 backwashing Methods 0.000 description 1
- 230000037208 balanced nutrition Effects 0.000 description 1
- 235000019046 balanced nutrition Nutrition 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 230000003749 cleanliness Effects 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000010030 laminating Methods 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 239000011241 protective layer Substances 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000002344 surface layer Substances 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention provides a kind of bioreactors, it is cultivated for the adventitious root to biology, the reactor includes tank body, the bottom of the tank body is provided with air sparger, the air sparger includes the ontology snapped together and disk, the ontology includes the interface structure being connected to reactor outside, and the gas outside the reactor passes sequentially through the interface structure, the disk enters in the tank body, and the disk includes at least metal sintering stratum reticulare.Bioreactor provided by the invention can realize that large-scale breeding is cultivated to ginseng adventitious root, to produce the high industrialization ginseng adventitious root product of stable quality, qualification rate.Bioreactor in the present invention only needs to carry out centainly being transformed on general fermentation tank to can be used to ginseng adventitious root breeding, and reactor low cost is easy to get, and reduces the production cost in ginseng adventitious root cultivating process, and large-scale promotion is suitble to use.
Description
Technical Field
The invention relates to the field of biological cultivation devices, in particular to a bioreactor.
Background
Along with the development of society, the living standard of people is continuously improved, and people pay more and more high attention to health preservation. Ginseng, as a tonic holy product, is increasingly sought after. The ginseng is cultured in seedling transplanting or direct seeding mode, and the growth speed is slow, so that the continuously increasing demands of people on ginseng and ginseng adventitious roots cannot be met.
In the prior art, the culture of the ginseng adventitious roots is basically in a laboratory stage, reactors used in the process of culturing the ginseng adventitious roots are all small bioreactors with a laboratory scale, and the volume of the reactor used for single culture is between 5 and 20 liters. Most of the small bioreactors for experiments are glass airlift tanks or PC airlift barrels, which are small in size and simple in equipment, and can not realize efficient culture of the adventitious roots of the ginseng.
Disclosure of Invention
The invention aims to provide a bioreactor which has large culture scale, high culture efficiency and high qualification rate of cultured adventitious roots, aiming at the problems of small propagation quantity and low propagation efficiency of the adventitious roots of ginseng in the prior art.
In order to solve the technical problems, the invention adopts the following technical scheme:
a bioreactor is used for culturing adventitious roots of organisms and comprises a tank body, an air distributor is arranged at the bottom of the tank body and comprises a body and a disk surface which are buckled together, the body comprises an interface structure communicated with the outside of the reactor, gas outside the reactor sequentially passes through the interface structure and the disk surface and enters the tank body, and the disk surface at least comprises a metal sintering mesh layer.
Preferably, the body comprises a curved surface structure with a downward concave middle part, a cavity is formed between the body and the disk surface, the body and the disk surface form a detachable fixed connection through a fixing structure, and/or,
the body is made of a metal material.
Preferably, the fixing structure includes a first fixing member and a second fixing member, the first fixing member is connected to the body and located on one side of the body away from the disk surface, the second fixing member is connected to the disk surface and located on one side of the disk surface away from the body, and the first fixing member and the second fixing member are fixed together by a fastening member to clamp the body and the disk surface between the first fixing member and the second fixing member.
Preferably, the first fixing member and the second fixing member are ring-shaped structures, and the first fixing member and the second fixing member are respectively located at edges of the body and the disk surface.
Preferably, the bioreactor further comprises a stirring device, the stirring device comprises a column structure extending from the top of the tank body to the bottom of the tank body,
the cylinder structure is sleeved with propeller type stirring blades at preset intervals.
Preferably, one end of the cylindrical structure at the bottom of the tank body is provided with a propeller type shearing blade.
Preferably, the stirring device further comprises a power component, the power component is fixedly connected with one end, located at the top of the tank body, of the column structure, and the power component drives the column structure to rotate and/or swing so as to disturb the adventitious roots in the tank body.
Preferably, the wall structure of the bottom of the tank body is in an inverted cone shape, the air distributor is arranged at the bottom end of the inverted cone shape, and the interface structure extends out of the tank body and is connected with a ventilation pipeline.
Preferably, the wall structure of the bottom of the tank is provided with a transfer outlet for removing the adventitious roots located at the bottom of the tank from the tank, and/or,
and the wall surface structure at the top of the tank body is provided with an inoculation inlet, and the inoculation inlet is used for inoculating the adventitious roots into the tank body.
Preferably, the wall surface structure at the top of the tank body is provided with an air outlet channel structure, and the air outlet channel structure comprises a condensation pipe extending vertically.
The bioreactor provided by the invention can realize large-scale propagation and cultivation of the ginseng adventitious roots so as to produce industrialized ginseng adventitious root products with stable quality and high qualification rate.
The bioreactor can be used for the propagation of the adventitious roots of the ginseng only by carrying out certain transformation on a general fermentation tank, has low manufacturing cost and easy acquisition, reduces the production cost in the cultivation process of the adventitious roots of the ginseng, and is suitable for large-scale popularization and use.
Drawings
FIG. 1 is a schematic view showing the overall structure of a bioreactor provided in example 1 of the present invention;
fig. 2 is a schematic view showing a mounting structure of the fixing structure provided in embodiment 1 of the present invention.
Wherein,
1. a tank body, 11, a tank body, 12, a wall surface structure, 13, an inoculation inlet,
2. air distributor, 21, body, 211, interface structure, 22, disk surface, 23, cavity,
3. a support frame is arranged on the base plate,
4. a ventilation pipeline is arranged on the top of the shell,
5. a fixed structure 51, a first fixed part 52, a second fixed part,
6. stirring device, 61, cylinder structure, 62, power unit, 63, propeller type stirring blade, 64, propeller type shearing blade, 641, shearing edge,
7. and an air outlet channel structure, 71, a condensation pipe.
Detailed Description
The bioreactor provided by the invention is further explained in detail with reference to the attached drawings.
Description of the drawings: the present invention is described with reference to the orientation shown in fig. 1.
Example 1
As shown in FIGS. 1 and 2, the present embodiment provides a bioreactor for culturing adventitious roots of a living being, such as ginseng. The reactor comprises a tank body 1, wherein the tank body 1 comprises a tank body 11, and the tank body 1 is made of a metal material, preferably a high-temperature-resistant and corrosion-resistant metal material, such as aluminum alloy or high-speed tool steel. The wall structure 12 at the bottom of the tank body 1 is in an inverted cone shape, the wall structure 12 at the bottom of the tank body 1 and the tank body 11 in the inverted cone shape can be in an integral structure or in a split structure, and when the wall structure and the tank body are in the split structure, the wall structure and the tank body are preferably fixed together by adopting a welding mode. The bottom of the tank body 1 is also provided with a support 3 to support the tank body 1, the support 3 and the tank body 1 are preferably fixed together in a welding mode to enhance the strength between the two, prevent that the weight is larger after culture solution and adventitious roots are added into the tank body 1, and the problem of damage of the support 3 due to incapability of bearing is solved.
Further, the bottom of the tank body 1 is provided with an air distributor 2, and in this embodiment, the air distributor 2 is arranged at the bottom end of the inverted cone. Since the adventitious roots in the tank body 1 grow up gradually during the cultivation process, the weight of the adventitious roots also increases gradually, and thus the adventitious roots may fall under their own weight. The wall surface structure 12 at the bottom of the tank body 1 is designed to be inverted cone-shaped, so that the indefinite roots can be more compactly concentrated at the bottom of the inverted cone-shaped wall surface structure 12. Simultaneously, because air distributor 2 sets up the bottom of back taper makes the bubble that air distributor 2 blown out can be better will subside the jar body 1 bottom adventitious root blow once more to the well upper portion of the jar body 1 guarantees that adventitious root is in it is more even to disperse in the jar body 1 to guarantee that each adventitious root can both be in absorb balanced nutrition in the jar body 1, and then guarantee to cultivate the reliability, improve the cultivation qualification rate of adventitious root.
The air distributor 2 comprises a body 21 and a disk surface 22 which are buckled together, the body 21 comprises an interface structure 211 communicated with the outside of the reactor, the interface structure 211 extends out of the tank body 1 and is connected with an air vent pipeline 4, and clean air or other gases required in the process of culturing adventitious roots can be introduced into the air vent pipeline 4. A control valve (not shown in the figure) is arranged in the interface structure 211, and the control valve is connected with a control device (not shown in the figure) of the bioreactor, and the control device can control the opening and closing of the control valve so as to control the on-off of the air flow in the interface structure 211. The gas outside the reactor enters the tank body 1 through the interface structure 211 and the disc surface 22 in sequence, and the disc surface 22 at least comprises a metal sintered mesh layer. The metal sintering net layer is manufactured by combining a plurality of layers of metal wires through special processes of laminating pressing, vacuum sintering and the like. The metal sintered mesh layer comprises five layers, a filtering layer, a protective layer, a supporting layer, a separating layer and a reinforcing layer, and the metal material for manufacturing the metal sintered mesh layer in the embodiment is preferably high-speed tool steel. The metal sintering net layer not only has uniform and stable filtering precision, but also has higher strength and rigidity, and simultaneously has higher compressive strength and filtering granularity. The metal sintering net layer has excellent backwashing regeneration performance due to the fact that the surface layer filtration is adopted as the filtration principle of the metal sintering net layer, and the mesh pore canal formed by the metal wires is smooth, can be repeatedly used for a long time, and can be cleaned by methods of backflow water, filtrate, ultrasonic waves, melting, baking and the like. Meanwhile, the metal sintered mesh material forming the metal sintered mesh layer is easy to form, can be processed and welded, and can be conveniently processed and manufactured into various forms of filter elements such as round, cylindrical, conical and corrugated. The metal sintering net has wide application range, can be applied to filtering in a temperature environment of-200-600 ℃ and an acid-base environment, is particularly suitable for continuous and automatic operation processes, and cannot be compared with any filtering material.
Further, the body 21 of the air distributor 2 comprises a curved structure with a downward-depressed middle part, a cavity 23 is formed between the body 21 and the disc surface 22, the body 21 and the disc surface 22 are detachably and fixedly connected through a fixing structure 5, and in order to ensure the use reliability of the air distributor 2 and prevent the air distributor 2 from being corroded or leaking air, the body 21 is made of a metal material, and preferably, the body 21 is supported by high-speed tool steel. The interface structure 211 and the body 21 are separate structures, and the interface structure 211 and the body 21 are preferably fixed together by welding. The interface structure 211 and the body 21 adopt a split structure, so that the application range of the air distributor 2 is wider, and the interface structures with different sizes are welded on the body 21 according to different devices to be connected with the air distributor 2. Of course, it is understood that the body 21 and the interface structure 211 may be a unitary structure, and may be integrally formed by casting, so as to facilitate manufacturing. The gas entering the air distributor 2 from the interface structure 211 is stored for a certain period of time in the cavity 23 formed between the body 21 and the plate surface 22, therefore, means such as having a filtering function or having an increased oxygen content in the gas can be preferably provided in the cavity 23 to increase the cleanliness and oxygen content of the gas entering the tank 1, so as to achieve a better incubation effect and to make the cultivated adventitious roots higher in quality.
As shown in fig. 2, the fixing structure 5 includes a first fixing member 51 and a second fixing member 52, the first fixing member 51 is connected to the body 21 and located on a side of the body 21 away from the disk surface 22, the second fixing member 52 is connected to the disk surface 22 and located on a side of the disk surface 22 away from the body 21, and the first fixing member 51 and the second fixing member 52 are fixed together by a fastening member (not shown in the figure) to clamp the body 21 and the disk surface 22 between the first fixing member 51 and the second fixing member 52. The first fixing member 51 and the second fixing member 52 are annular structures, and the first fixing member 51 and the second fixing member 52 are respectively located at the edges of the main body 21 and the disk surface 22. Preferably, the fastener uses screw and bolt to guarantee the convenience of dismantlement and installation, can also guarantee good fastening effect simultaneously. The body 21 and the disk surface 22 are fixed together through the fixing structure 5, and can be very conveniently disassembled and assembled, so that the air distributor 2 can be conveniently cleaned or overhauled after being used for a period of time, and the using effect and the using reliability of the air distributor 2 can be guaranteed.
As shown in fig. 1, the bioreactor further comprises a stirring device 6, wherein the stirring device 6 comprises a column structure 61 extending from the top of the tank 1 to the bottom of the tank 1, and a power component 52. The power component 62 is fixedly connected with one end of the column structure 61, which is positioned at the top of the tank body 1, and the power component 62 drives the column structure 61 to rotate and/or swing so as to disturb an indefinite root in the tank body 1. The cylinder structure 61 is sleeved with propeller type stirring blades 63 at intervals of a preset length, and when the cylinder structure 61 rotates, the propeller type stirring blades 63 rotate under the driving of the cylinder structure 61; when the column structure 61 is swung by the power unit 62, the propeller stirring blade 63 is also swung while rotating. Preferably, the power component 62 comprises an electric motor, preferably an electric motor capable of outputting both torque and amplitude to simultaneously effect rotation and vibration of the cylindrical structure. The shape of the column structure 61 is not limited, and the column structure may be a cylinder, a square column, or a special-shaped column, so as to facilitate installation and use as a standard. The specific position of the column structure 61 extending to the bottom of the tank body 1 is set according to the volume and height of the tank body 1, preferably, one end of the column structure 61 located at the bottom of the tank body 1 is set at the junction position of the tank body 11 and the inverted cone-shaped wall surface structure 12, so as to ensure that all the positions in the tank body 1 can feel disturbance at different positions. The number of the propeller-type stirring blades 63 is not too large, and the distance between two adjacent propeller-type stirring blades 63 is not too large, so as to prevent unnecessary damage to adventitious roots. Because the stirring blades are of a propeller type blade structure, the mechanical damage of shearing force to the adventitious roots of the ginseng during the rotation and swing processes of the propeller type stirring blades 63 can be effectively reduced, and the cultivation efficiency and the cultivation quality are ensured. Meanwhile, the dissolved oxygen environment in the culture solution in the tank body 1 can be enhanced by the rotation of the propeller type stirring blades 63, so that a better culture environment is created, and adventitious roots with better quality are cultured.
Further, as the adventitious roots grow up, the volume and the weight of the adventitious roots become larger and larger, the adjacent adventitious roots are interwoven with each other, and in order to obtain the adventitious roots with superior quality, the adventitious roots growing to a certain extent in the tank body 1 need to be transferred to a bioreactor with a larger volume for continuous culture and breeding. Therefore, one end of the cylinder structure at the bottom of the tank body 1 is provided with a propeller type shearing blade 64, and the propeller type shearing blade 64 continuously rotates during the continuous rotation of the cylinder structure 61 to shear the adventitious roots, so as to conveniently transfer the sheared adventitious roots to a larger bioreactor for culture. The air distributor introduces air into the tank body 1 from bottom to top, the adventitious roots at the bottom of the tank body 1 are changed from a gathering state to a dispersing state under the driving of the air flow, the propeller type stirring blades 63 rotate to enhance the dispersing effect of the adventitious roots, and in the process, the propeller type shearing blades 64 rotate to shear the dispersed adventitious roots so as to achieve a better shearing effect. Preferably, the propeller type cutting blade 64 has four cutting edges 641 to ensure that the cutting effect is better when the cutting is performed on the indefinite roots in multiple directions at the same time.
In order to facilitate the transfer of the sheared adventitious roots, the wall structure 12 at the bottom of the tank 1 is provided with a transfer outlet (not shown in the figure) for removing the adventitious roots at the bottom of the tank 1 from the tank 1 and transferring the adventitious roots to a larger reactor. A seeding inlet 13 is arranged on the wall surface structure 12 at the top of the tank body 1, and the seeding inlet 13 is used for seeding the adventitious roots into the tank body 1.
Furthermore, an air outlet channel structure 7 is arranged on the wall surface structure 12 at the top of the tank body 1, and the air outlet channel structure 7 comprises a condensation pipe 71 extending along the vertical direction. The air outlet channel structure 7 can discharge metabolic gas generated in the growth and development process of the adventitious roots from the interior of the tank body 1 so as to ensure the cleanness of the environment in the tank body 1 and obtain a better culture and propagation environment. The condensation duct 71 is capable of condensing the gas discharged from the gas outlet passage structure 7 and discharging the condensed water from the condensation duct 71.
Example 2
The difference between this embodiment and embodiment 1 is that the body and the disk surface of the air distributor are fixed together by welding or fasteners, and do not need to be fixed by a fixing structure.
Furthermore, a filtering device is disposed in the air outlet channel structure in this embodiment, the filtering device includes a drying portion and an adsorbing portion, and the drying portion is configured to adsorb water vapor in the gas exhausted from the tank body, so as to ensure that the gas exhausted to the outside of the tank body is dry, and prevent the environment outside the tank body from being too humid. The adsorption part is used for adsorbing harmful substances in the gas exhausted from the tank body so as to ensure that the air outside the tank body is purified and cannot pollute the environment.
Claims (10)
1. The utility model provides a bioreactor for cultivate biological adventitious root, the reactor includes a jar body, the bottom of jar body is provided with air distributor, its characterized in that: the air distributor comprises a body and a disc surface which are buckled together, the body comprises an interface structure communicated with the outside of the reactor, gas outside the reactor enters the tank body through the interface structure and the disc surface in sequence, and the disc surface at least comprises a metal sintering mesh layer.
2. The bioreactor of claim 1, wherein: the body comprises a curved surface structure with a downward sunken middle part, a cavity is formed between the body and the disc surface, the body and the disc surface form a detachable fixed connection through a fixed structure, and/or,
the body is made of a metal material.
3. The bioreactor of claim 2, wherein: the fixing structure comprises a first fixing piece and a second fixing piece, the first fixing piece is connected with the body and is positioned on one side, far away from the plate surface, of the body, the second fixing piece is connected with the plate surface and is positioned on one side, far away from the body, of the plate surface, and the first fixing piece and the second fixing piece are fixed together through a fastening piece so as to clamp the body and the plate surface between the first fixing piece and the second fixing piece.
4. A bioreactor according to claim 3, wherein: the first fixing piece and the second fixing piece are of annular structures and are respectively positioned at the edges of the body and the disc surface.
5. The bioreactor of claim 1, further comprising a stirring device comprising a cylindrical structure extending from the top of the tank to the bottom of the tank,
the cylinder structure is sleeved with propeller type stirring blades at preset intervals.
6. The bioreactor of claim 5, wherein: one end of the column structure, which is positioned at the bottom of the tank body, is provided with a propeller type shearing blade.
7. The bioreactor of claim 6, wherein: the stirring device further comprises a power component, the power component is fixedly connected with one end, located at the top of the tank body, of the column body structure, and the power component drives the column body structure to rotate and/or swing so as to disturb the adventitious roots in the tank body.
8. The bioreactor of claim 1, wherein the wall structure of the bottom of the tank body is in an inverted cone shape, the air distributor is arranged at the bottom end of the inverted cone shape, and the interface structure extends out of the tank body and is connected with an air vent pipeline.
9. The bioreactor of claim 1, wherein: a transfer outlet is arranged on the wall surface structure at the bottom of the tank body and is used for removing the adventitious roots at the bottom of the tank body from the tank body and/or,
and the wall surface structure at the top of the tank body is provided with an inoculation inlet, and the inoculation inlet is used for inoculating the adventitious roots into the tank body.
10. The bioreactor of claim 1, wherein: the wall structure at the top of the tank body is provided with an air outlet channel structure, and the air outlet channel structure comprises a condensation pipe extending vertically.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810398406.8A CN108522247A (en) | 2018-04-28 | 2018-04-28 | A kind of bioreactor of culture ginseng adventitious root |
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| CN201810398406.8A CN108522247A (en) | 2018-04-28 | 2018-04-28 | A kind of bioreactor of culture ginseng adventitious root |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022206423A1 (en) * | 2021-04-02 | 2022-10-06 | 山东安然纳米实业发展有限公司 | Biological reaction device and culture method for adventitious roots of panax ginseng c. a. mey. |
| CN117256478A (en) * | 2023-11-22 | 2023-12-22 | 上海科缔联科技有限公司 | Continuous fermentation culture system for plant tissues |
| CN117256479A (en) * | 2023-11-22 | 2023-12-22 | 上海科缔联科技有限公司 | Industrial automatic biological reaction device and control method thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0775549A (en) * | 1993-09-09 | 1995-03-20 | Hitachi Ltd | Living body cell culture arrangement |
| KR200232169Y1 (en) * | 2001-02-23 | 2001-07-19 | 백기엽 | the medium animate of air floating drum |
| KR200245301Y1 (en) * | 2001-06-13 | 2001-10-17 | 재단법인 한국인삼연초연구원 | The culturing apparatus for panax ginseng roots |
| CN1680540A (en) * | 2005-01-13 | 2005-10-12 | 上海交通大学 | Culturing system of built-in light biological reactor of plant tissue cutter |
| CN1680538A (en) * | 2005-01-26 | 2005-10-12 | 清华大学 | Composite preparing device of circulating and filling strain preparation and biological carrier |
| CN201027184Y (en) * | 2007-03-02 | 2008-02-27 | 广州市金润环保科技有限公司 | Three-phase fluidization algae photo-bioreactor |
| CN202427427U (en) * | 2012-01-10 | 2012-09-12 | 宁波中一石化科技有限公司 | Sintered mesh type micro-porous gas distributor |
-
2018
- 2018-04-28 CN CN201810398406.8A patent/CN108522247A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0775549A (en) * | 1993-09-09 | 1995-03-20 | Hitachi Ltd | Living body cell culture arrangement |
| KR200232169Y1 (en) * | 2001-02-23 | 2001-07-19 | 백기엽 | the medium animate of air floating drum |
| KR200245301Y1 (en) * | 2001-06-13 | 2001-10-17 | 재단법인 한국인삼연초연구원 | The culturing apparatus for panax ginseng roots |
| CN1680540A (en) * | 2005-01-13 | 2005-10-12 | 上海交通大学 | Culturing system of built-in light biological reactor of plant tissue cutter |
| CN1680538A (en) * | 2005-01-26 | 2005-10-12 | 清华大学 | Composite preparing device of circulating and filling strain preparation and biological carrier |
| CN201027184Y (en) * | 2007-03-02 | 2008-02-27 | 广州市金润环保科技有限公司 | Three-phase fluidization algae photo-bioreactor |
| CN202427427U (en) * | 2012-01-10 | 2012-09-12 | 宁波中一石化科技有限公司 | Sintered mesh type micro-porous gas distributor |
Non-Patent Citations (1)
| Title |
|---|
| 丁启圣等: "生命科学前沿技术与中医药研究", vol. 1, 31 October 1999, 中国医药科技出版社, pages: 129 - 130 * |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2022206423A1 (en) * | 2021-04-02 | 2022-10-06 | 山东安然纳米实业发展有限公司 | Biological reaction device and culture method for adventitious roots of panax ginseng c. a. mey. |
| CN117256478A (en) * | 2023-11-22 | 2023-12-22 | 上海科缔联科技有限公司 | Continuous fermentation culture system for plant tissues |
| CN117256479A (en) * | 2023-11-22 | 2023-12-22 | 上海科缔联科技有限公司 | Industrial automatic biological reaction device and control method thereof |
| CN117256478B (en) * | 2023-11-22 | 2024-02-02 | 上海科缔联科技有限公司 | Continuous fermentation culture system for plant tissues |
| CN117256479B (en) * | 2023-11-22 | 2024-02-06 | 上海科缔联科技有限公司 | Industrial automatic biological reaction device and control method thereof |
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