CN108508074A - A kind of anti-interference dual-port screen printing electrode - Google Patents
A kind of anti-interference dual-port screen printing electrode Download PDFInfo
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- CN108508074A CN108508074A CN201810405406.6A CN201810405406A CN108508074A CN 108508074 A CN108508074 A CN 108508074A CN 201810405406 A CN201810405406 A CN 201810405406A CN 108508074 A CN108508074 A CN 108508074A
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
- G01N27/3272—Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels
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Abstract
The present invention relates to electrochemical method detection technique fields, provide a kind of anti-interference dual-port screen printing electrode, electrode base board including insulation, electrode base board is equipped with detecting electrode component, detecting electrode component includes two electrode layers for being set up in parallel and extending along the length direction of electrode base board, two electrode layers are respectively working electrode and reference electrode, reaction enzyme layer is covered with above the middle part of working electrode, the outside for reacting enzyme layer is equipped with lateral chromatography layer, the intersection for reacting enzyme layer and lateral chromatography layer is equipped with selectivity through film, the stage casing of electrode layer is covered with insulating layer, insulating layer also covers the part of counter electrode layer on lateral chromatography layer, inserted terminal positioned at all electrode layer pins of the same end collectively constitutes an electrode ports.Anti-interference dual-port screen printing electrode provided by the invention is convenient and efficient, the response time is short, has good anti-interference ability, can effectively reduce the cost of silk-screen printing, reduces printing complexity.
Description
Technical field
The present invention relates to electrochemical technology fields, are specifically related to a kind of anti-interference dual-port screen printing electrode.
Background technology
It is vital that small biological molecule can be rapidly and sensitively detected in clinical diagnostic applications.Wherein, blood glucose, Portugal
The detection of grape sugar and uric acid is particularly significant.Blood glucose value directly reflects that the degree of practical carbohydrate metabolism disturbance, glycemic control refer to whole day
The overall control of blood glucose;Glucose is indispensable substance in vital movement, it can directly participate in metabolism in human body
Process;Uric acid (UA) is present in blood and urine, and higher or lower uric acid level may lead to disease.Thus for this three
The Fast Detection Technique of kind molecule causes extensive concern.Electrochemical methods be considered as a kind of detection blood glucose, glucose,
The potential method of uric acid because showed in electrode surface these molecules it is electroactive, easily by redox.
Mainly there are enzyme coupled assay and high performance liquid chromatography, but high performance liquid chromatography in both methods at present
Detect relatively complicated, cost is higher, and existing enzyme coupled assay is less, mostly uses single electrochemical electrode test, such
Electrochemical electrode limitation is bigger, and detection object is single, and cost is higher and anti-interference ability is poor.
In the biological sample of human body, coexist high concentration many kinds of substance have with its extremely similar electrochemical properties,
Necessarily cause to interfere accordingly during measurement.So developing, at low cost, manufacture craft is simple, highly sensitive, Gao Xuan
The electrochemical sensor for measuring blood glucose, glucose, uric acid of selecting property is as people's urgent problem to be solved.
Invention content
The technical problem to be solved by the present invention is to:In view of the deficienciess of the prior art, providing one kind having good resist
Interference performance can effectively reduce the cost of silk-screen printing, reduce the anti-interference dual-port silk-screen printing electricity of printing complexity
Pole.
In order to solve the above technical problems, the technical scheme is that:A kind of anti-interference dual-port screen printing electrode, packet
The electrode base board of insulation is included, the electrode base board is equipped with detecting electrode component, and the detecting electrode component includes two arranged side by side
It is arranged and along the electrode layer that the length direction of the electrode base board extends, two electrode layers is respectively working electrode and reference
Electrode, reaction enzyme layer is covered with above the middle part of the working electrode, and the outside of the reaction enzyme layer is equipped with lateral chromatography layer, institute
The intersection for stating reaction enzyme layer and the lateral chromatography layer is equipped with selectivity through film, and the stage casing of the electrode layer is covered with insulation
Layer, the insulating layer also cover the part that the electrode layer is corresponded on the lateral chromatography layer, are located at all described of the same end
The inserted terminal of electrode layer pin collectively constitutes an electrode ports.
Preferably, the reaction enzyme layer is one kind in glucose oxidase, hexokinase and urate oxidase.
Preferably, the lateral chromatography layer is the hydrophilic material that can form siphon passage.
Preferably, the hydrophilic material is water-insoluble polysaccharide polymer, hydrophily chromatographic resin and macropore polypropylene
One kind in acid esters.
Preferably, the electrode layer is the mixture of one or both of conductive carbon paste and conductive silver paste.
Preferably, the insulating layer is dielectric ink.
Preferably, the selectivity through film by semi-permeable membrane and to interfering substance there is the molecule of selective absorption effect to print
Mark chitosan film is sticked to form.
Preferably, the tow sides of the electrode base board are equipped with the detecting electrode component.
Preferably, the middle part of the electrode base board is equipped with water board, and the water board is by hydrophilic plant fiber
It is made, and through the tow sides of the electrode base board.
By adopting the above-described technical solution, the beneficial effects of the invention are as follows:
Solution to be detected can be guided to instead by the lateral chromatography layer of the anti-interference dual-port screen printing electrode of the present invention
It answers at enzyme layer, while lateral chromatography layer can effectively make ingredient to be detected logical with the selectivity reacted between enzyme layer through film
It crosses, while chaff interferent is isolated;The electrode layer that electrode base board is equipped with for conduction, gather around there are two electrode ports can be directed to it is same
Band detection sample is quickly detected, and two testing results can more analyze the accuracy of testing result.
The reaction enzyme layer being equipped with can react with ingredient to be detected, generate induced current, you can obtain it is to be detected at
The concentration divided, the convenient and efficient response time is short, when reaction enzyme layer is glucose oxidase, can detect the concentration of glucose;Reaction
When enzyme layer is hexokinase, the concentration of blood glucose (including glucose, mannose, Glucosamine and fructose) can detect;Reaction enzymes
When layer is urate oxidase, the concentration of uric acid can detect.
The lateral chromatography layer water conservancy diversion effect that hydrophilic material by that can form siphon passage is constituted is more preferable;By conductive carbon paste and
The electrode layer of the mixture composition of one or both of conductive silver paste has excellent electric conductivity.
There is the selectivity that the molecular engram chitosan film of selective absorption effect forms by semi-permeable membrane and to interfering substance
Through film, can selectivity by component molecules to be detected and the molecule smaller than component molecules to be detected, while molecular engram
Chitosan film can adsorb the substance that interference is generated to ingredient to be detected, keep testing result more accurate.
The tow sides of electrode base board are equipped with detecting electrode component, and anti-interference dual-port screen printing electrode may be implemented
Two-sided use, reduce the use of electrode base board, while improving detection efficiency, reduce printing cost.
Anti-interference dual-port screen printing electrode provided by the invention is convenient and efficient, the response time is short, there have to be anti-dry well
Ability is disturbed, the cost of silk-screen printing can be effectively reduced, reduces printing complexity.
Description of the drawings
Fig. 1 is the overlooking structure diagram of anti-interference dual-port screen printing electrode in the embodiment of the present invention;
Fig. 2 is the A-A schematic cross-sectional views of the anti-interference dual-port screen printing electrode of Fig. 1;
Fig. 3 is the B-B schematic cross-sectional views of the anti-interference dual-port screen printing electrode of Fig. 1;
In figure:1- electrode base boards, 21- first electrodes port, 22- second electrodes port, 3- lateral chromatography layers, 4- insulation
Layer, 51- working electrodes, 52- reference electrodes, 6- selectively penetrate film, and 7- reacts enzyme layer, 8- water boards.
Specific implementation mode
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, and
It is not used in the restriction present invention.
Fig. 1-3 collectively illustrates the structural schematic diagram of anti-interference dual-port screen printing electrode provided by the invention, in order to
Convenient for explanation, this figure only provides structure division related to the present invention.
As shown in Figs. 1-3, anti-interference dual-port screen printing electrode, includes the electrode base board 1 of insulation, and electrode base board 1 is used
In carrying electrode other structures layer;Electrode base board 1 is equipped with detecting electrode component, and detecting electrode component includes at least two arranged side by side
It is arranged and along the electrode layer that the length direction of electrode base board 1 extends, electrode layer plays conductive effect, in order to make it easy to understand, with
For two electrode layers, two electrode layers are respectively working electrode 51 and reference electrode 52;It is covered above the middle part of working electrode 51
There are reaction enzyme layer 7, reaction enzyme layer 7 to be used to chemically react with the ingredient to be measured in detection stoste, generates induced current, detection
The electric current simultaneously carries out the concentration that analysis can be obtained ingredient to be measured, and the convenient and efficient and response time is short;
When the ingredient difference of first electrode 51 and the reaction enzyme layer 7 in second electrode 52, this has two-parameter detection work(
The dual-port screen printing electrode of energy then can be carried out at the same time detection to ingredient to be measured different in detection stoste, increase its detection
Diversity improves detection efficiency;
The outside for reacting enzyme layer 7 is equipped with lateral chromatography layer 3, and lateral chromatography layer 3 is used to receive detection stoste, and will detection original
Liquid layer analysis is guided in reaction enzyme layer 7 and is chemically reacted for it, generates response current;
The intersection for reacting enzyme layer 7 and lateral chromatography layer 3 is equipped with selectivity through film 6, and selectivity is through film 6 by semi-permeable membrane
With to interfering substance there is the molecular engram chitosan film of selective absorption effect to be sticked to form, selectivity is through film 6 by semi-transparent
Film and there is the molecular engram chitosan film of selective absorption effect to be sticked to form interfering substance, what semi-permeable membrane can be selective
Pass through ingredient to be detected and the molecule smaller than ingredient to be detected;Molecular engram chitosan film, which can adsorb, produces ingredient to be detected
The substance of raw interference, keeps testing result more accurate.
In this embodiment, it selects in water-insoluble polysaccharide polymer, hydrophily chromatographic resin and macropore polyacrylate
A kind of hydrophilic material made of lateral chromatography layer 3, then can form siphon passage, obtain preferably chromatography water conservancy diversion effect.
The stage casing of electrode layer is covered with insulating layer 4, and insulating layer 4 also covers counter electrode layer on lateral chromatography layer 3
Part then can effectively avoid detection stoste and accidentally be dripped or immerse 51 He of first electrode without lateral chromatography layer 3 and reaction enzyme layer 7
Second electrode 52 influences the accuracy of testing result, and the inserted terminal positioned at all electrode layer pins of the same end collectively constitutes one
A electrode ports add up to two electrode ports, that is, first electrode port 21 and second electrode port 22, can be directed to same to be measured
Ingredient is used for quickly detecting to obtain two groups of testing results, is convenient for comparative analysis detection data, further increases the standard of testing result
True property.
Electrode layer made of mixture using one or both of conductive carbon paste and conductive silver paste, then so that electrode layer
With excellent electric conductivity.
It is respectively provided with above-mentioned detecting electrode component in the tow sides of electrode base board 1, the two-sided of electrode base board 1, which may be implemented, to be made
With increasing the access times of the dual-port screen printing electrode for having anti-interference function, reduce the wave of 1 consumptive material of electrode base board
Take, while improving detection efficiency, reduces printing cost.
The middle part of electrode base board 1 is equipped with water board 8, and water board 8 is made of hydrophilic plant fiber, and through electricity
The tow sides of electrode substrate 1, water board 8 can effectively adsorb detection stoste, and in the siphonage of lateral chromatography layer 3
Under, it realizes reacting between the reaction enzyme layer 7 of tow sides and detection stoste, realizes anti-interference dual-port screen printing electrode
Tow sides detect, and do not have to that detection stoste is added dropwise repeatedly, operation is more convenient.
Following embodiment be by two electrode layers have the dual-port screen printing electrode of two-parameter detection function for, needle
Ingredient to be detected ingredient to be detected ingredient to be detected is illustrated to the different situations of the reaction enzymes used on each electrode layer.
Embodiment 1
In the anti-interference dual-port screen printing electrode that the present embodiment is provided, electrode base board 1 is polyester film PET sheet, side
It is the water-insoluble polysaccharide polymer film that can form siphon passage to chromatography layer 3, electrode layer is conductive carbon paste and conductive silver paste
Mixture, reaction enzyme layer 7 are glucose oxidase;Selectivity is through film 6 by point of glucose semi-permeable membrane and glucose chaff interferent
Sub- blotting chitosan film composition, operation principle are as follows:
(1) it takes detection stoste one to drip, instills lateral chromatography layer 3, detection chromatography water conservancy diversion of the stoste through lateral chromatography layer 3 is laggard
Selectable penetrates film 6, and when penetrating film 6 by selectivity, the molecular engram chitosan film of glucose chaff interferent adsorbs grape
Sugared chaff interferent, dextrose components then contact generation chemistry instead by reacting enzyme layer 7 after selective semi-permeable membrane 6 with glucose oxidase
It answers;
(2) first electrode port 21 is detected by electrochemical workstation and obtains electric current I1;
(3) second electrode port 22 is detected by electrochemical workstation and obtains electric current I2;
(4) made the difference by the electric current to two ports and get response current I=| I1-I2|。
Response current I1And I2The electric current of generation, warp are reacted with glucose oxidase by glucose in same detection stoste
It crosses analysis and can be calculated two groups of detection datas, response current difference I represents the testing result of concentration of glucose in detection stoste
Accuracy, I values are smaller, and accuracy is higher, and I values are smaller, i.e., this testing result is more accurate.
Embodiment 2
The structure for the anti-interference dual-port screen printing electrode that the present embodiment is provided is substantially the same manner as Example 1, no
It is with place:Lateral chromatography layer is the hydrophily chromatography resin film that can form siphon passage;Selectivity is through film 6 by that can pass through
Glucose, mannose, Glucosamine and the semi-permeable membrane of fructose and the molecular engram chitosan film composition of chaff interferent;React enzyme layer
7 be hexokinase.
Its operation principle is substantially the same manner as Example 1, can effectively detect detection stoste in blood glucose (including glucose,
Mannose, Glucosamine and fructose) concentration.
Embodiment 3
The structure for the anti-interference dual-port screen printing electrode that the present embodiment is provided is substantially the same manner as Example 1, no
It is with place:Lateral chromatography layer 3 is the macropore polyacrylate film that can form siphon passage;Enzyme layer 7 is reacted to aoxidize for uric acid
Enzyme;Selectivity is made of through film 6 the molecular engram chitosan film of uric acid semi-permeable membrane and uric acid chaff interferent.
Its operation principle is substantially the same manner as Example 1, can effectively detect the concentration of the uric acid in detection stoste.
Anti-interference dual-port screen printing electrode provided by the invention is convenient and efficient, the response time is short, there have to be anti-dry well
Ability is disturbed, the cost of silk-screen printing can be effectively reduced, reduces printing complexity.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement etc., should all be included in the protection scope of the present invention made by within refreshing and principle.
Claims (9)
1. a kind of anti-interference dual-port screen printing electrode, the electrode base board of insulation, it is characterised in that:It is set on the electrode base board
There are detecting electrode component, the detecting electrode component to include two and be set up in parallel and extend along the length direction of the electrode base board
Electrode layer, two electrode layers are respectively working electrode and reference electrode, are covered with above the middle part of the working electrode
Enzyme layer is reacted, the outside of the reaction enzyme layer is equipped with lateral chromatography layer, the boundary of the reaction enzyme layer and the lateral chromatography layer
Place is equipped with selectivity and penetrates film, and the stage casing of the electrode layer is covered with insulating layer, and the insulating layer also covers the lateral chromatography
The part that the electrode layer is corresponded on layer, the inserted terminal positioned at all electrode layer pins of the same end collectively constitute an electricity
Extreme mouth.
2. anti-interference dual-port screen printing electrode as described in claim 1, it is characterised in that:The reaction enzyme layer is glucose
One kind in oxidizing ferment, hexokinase and urate oxidase.
3. anti-interference dual-port screen printing electrode as described in claim 1, it is characterised in that:The lateral chromatography layer is can shape
At the hydrophilic material of siphon passage.
4. anti-interference dual-port screen printing electrode as claimed in claim 3, it is characterised in that:The hydrophilic material be water not
One kind in soluble polysaccharide polymer, hydrophily chromatographic resin and macropore polyacrylate.
5. anti-interference dual-port screen printing electrode as described in claim 1, it is characterised in that:The electrode layer is conductive carbon paste
With the mixture of one or both of conductive silver paste.
6. anti-interference dual-port screen printing electrode as described in claim 1, it is characterised in that:The insulating layer is insulating oil
Ink.
7. anti-interference dual-port screen printing electrode as described in claim 1, it is characterised in that:The selectivity through film by
Semi-permeable membrane and to interfering substance have selective absorption effect molecular engram chitosan film be sticked to form.
8. anti-interference dual-port screen printing electrode as described in any one of claim 1 to 7, it is characterised in that:The electrode
The tow sides of substrate are equipped with the detecting electrode component.
9. anti-interference dual-port screen printing electrode as claimed in claim 8, it is characterised in that:The middle part of the electrode base board
Equipped with water board, the water board is made of hydrophilic plant fiber, and through the tow sides of the electrode base board.
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN113899899A (en) * | 2021-09-22 | 2022-01-07 | 浙江大学 | Virus detection device based on full-screen printing OECT and preparation and detection methods thereof |
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