CN1085046A - The enzymatic hydrolysis processing technology and the goods thereof of bird's nest - Google Patents
The enzymatic hydrolysis processing technology and the goods thereof of bird's nest Download PDFInfo
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Abstract
The enzymatic hydrolysis processing method of a kind of bird's nest and waste residue thereof.Can adopt various plants protease, bacterialprotease, animal protease or reagent type protease, also can adopt two or more complex enzyme.This genealogy of law will be through rinsing and the pretreated raw material of poach, adjust its pH value under near neutrality or meta-alkalescence condition earlier through 1.5 to 3.5 hours be lower than 65 ℃ of constant temperature hydrolytic processes and hydrolytic process is boiled in intensification, the gained hydrolyzate after filtration, centrifugal and dry after make and contain moisture and be not more than 5% white or buff powder goods.The product of making through this technology comprises the additive or the nutrition fortifier of pulvis goods, oral liquid, pill, cosmetics.This law reaction speed is fast, resulting product productive rate height, and process conditions are simple, can handle various types of bird's nest raw materials.
Description
The present invention relates to the hydrolysis processing method of bird's nest process technology, particularly bird's nest.
Bird's nest is the Apodidae animal, esculent swift and multiplely belong to the nest nest that the swallow class builds up with condensation by mixings such as saliva or saliva and downs together.The chemical analysis of bird's nest is very complicated, contains multiple proteins, and no nitrogen extract, fiber, ash content and trace fat contain about 10.4% moisture content in addition, and Main Ingredients and Appearance is calcium, phosphorus, potassium, sulphur etc. in its ash content.Also has a kind of particular matter-glycopeptide in the bird's nest.
So far, natural bird's nest is still continued to use manual method processing, be with the former bird's nest of adopting after the machinery rinsing of some roads, fragmentation, with manual operations its band hair part is separated with not being with the hair part.This original manual process, labour intensity is big, and efficient is low, and an experienced operators only can be handled the 50-60 gram every day.Particularly this manual separating step generally carries out three to five times at least repeatedly, all can cause the loss of a part of bird's nest entity at every turn, and whole process loss late is 35-40%, also will produce a large amount of bird's nest bits at last and fall as waste disposal.
Adopting Hydrolyze method to process some plant animal protein is applied at some food processing process.Chinese patent application CN90101832 has reported the method for producing poultry hydrolysate with papain, this method control hydrolytic process, can make the meat of poultry and muscles and bones etc. be regarded as protein in the waste part usually and become the hydrolysate of can be edible and being easy to absorb, improve the utilization rate of protein for people.But this method only limits to poultry, the particularly disposal of chicken and muscles and bones, and also available enzyme is subjected to special qualification.
The method of the hydrolysis processing bird's nest goods that task of the present invention is to provide a kind of process conditions simply can adopt the high concentration enzyme.
Above-mentioned task realizes with following technical process.
One. raw material give processing
1. get former bird's nest,, remove the down of surface contaminants and surface attachment through rinsing repeatedly especially for the hair swallow.As be half clean bird's nest or the bird's nest slag of handling, then can save this step.
2. above-mentioned raw materials was soaked 60-120 minute under 40 ° of-45 ℃ of conditions in hydrolyzer, it is fully absorbed water.
3. as soon as soak to finish, promptly be warmed up to boiling and keep boiling 20 minutes, at this moment, the water content of bird's nest should be more than 80%.
4. coarse filtration gained water cooking liquid is standby, and wet bird's nest (substrate) is weighed, and record.
Two. the bird's nest hydrolysis
5. aforementioned substrate is put into hydrolyzer, characteristic according to selected proteolytic enzyme, the pH value of adjusting substrate is to approaching neutral or inclined to one side alkali, stir and heat up, the control substrate temperature is below 65 ℃ and keep constant, reaction time is 0.5-2.0 hour, and reaction finishes evenly to be warming up to boiling point, and keeps ten minutes.
6. select proteolytic enzyme for use according to the difference of substrate, when substrate reaches constant temperature, add proteolytic enzyme or enzymolysis liquid, stir so that reaction evenly, is clocked.Will keep slow stirring in course of reaction, the reaction time is 1.5-3.5 hour.
The consumption of proteolytic enzyme is the 0.02-5.0% of substrate.Said proteolytic enzyme can be
Plant rennet is as bromelain, papain.
Bacterialprotease is as 1398 neutral proteinases.
Animal protease is as pepsin or trypsase etc.
Reagent type protease is as protease B.P or Proteinase K.Also can select two or more complex enzyme for use.
Above-mentioned enzymolysis liquid can be made with suitable water or aforementioned water cooking liquid.When using enzymolysis liquid to add in the substrate, water or water cooking liquid consumption should be no more than 20% of amount of substrate.
So far, most of substrate is hydrolyzed.
7. the above-mentioned stage of reaction of waiting finishes, and soon reactant liquor is warming up to boiling within 30 to 60 minutes gradually, and keeps boiling 10 minutes.
Also can take to add acid and stop the above-mentioned stage of reaction, add corresponding acid protease afterwards again and continue reactant liquor is warming up to boiling in the above-mentioned time, and boiled 10 minutes.
So far, can be by the almost all hydrolysis of the substrate of protease effect, hydrolyzate also will make protease lose activity through boiling.
Three. hydrolyzate is handled
8. hydrolyzate is used the filter cloth coarse filtration, with elimination down and the residues such as yellow micelle that can not be hydrolyzed.
9. again filtrate is placed centrifuge, centrifugal through 10 minutes, remove fine granularity insoluble solid such as protease in the filtrate, centrifugal rotational speed is not less than 4000 rev/mins.
10. adopt high-speed centrifugal or pressure spray dryer that the hydrolyzate after centrifugal is sprayed into powder.Also can adopt modes such as vacuum drying or freeze drying.The gained powder is water-soluble white or yellowly powder, and water content is not more than 5%.
Adopt foregoing invention, its goods are identified through physics and chemistry, glycopeptide content proof can keep the original nutritive value of bird's nest constant in pyrolysis gas chromatograph detection and the mensuration bird's nest.Enzymatic hydrolysis of the present invention can be widely used in handles former bird's nest, half clean bird's nest and the bird's nest slag adopted, it is many to have optional proteolytic enzyme kind, and directly add in the substrate, it is few that this more conventional enzymatic hydrolysis adds water, thereby the concentration of enzyme is enhanced about more than once, greatly the advantage of fast reaction speed than general technology.Adopt this law hydrolysis processing bird's nest goods to comprise the additive or the nutrition fortifier of pulvis goods, oral liquid, pill, cosmetics.Because the control hydrolytic process makes hydrolysis thorough, and can significantly improve labor productivity.
Further describe the present invention below in conjunction with accompanying drawing and by specific embodiment.
Fig. 1 is the process chart of enzymatic hydrolysis processing bird's nest method of the present invention.
Example one
Take by weighing half clean dried bird's nest (moisture content 12%), 100 grams, place the hydrolyzer that adds stirring with cover, add 1000 milliliters in water (PH 6.8).Stirring is warming up to 45 ℃, clocks, and soaks to be warming up to 100 ℃ rapidly after 60 minutes, and keeps boiling 20 minutes.The fully imbibition of substrate this moment.Elimination water cooking liquid (about 200 milliliters) is standby, the weighing substrate weighs 1020 grams, adjust the hydrolyzer temperature and remain on 58 °-60 ℃, substrate is reloaded in the hydrolyzer, take by weighing the PA-2 papain by the enzyme concentration of 10000 units/gram, and make 200 milliliters enzymolysis liquid with water cooking liquid, under agitation, join equably in the hydrolyzer, clock, and the observing response situation.Hydrolysis after 120 minutes most of substrate be hydrolyzed, after this begin to heat up, continue to stir, and be controlled to be warming up to after 30 minutes and boil, kept hydrolytic process end so far 10 minutes.Hydrolyzate leaches residue 30 grams (weight in wet base) through the filter cloth 900ml that filters to get filtrate.Centrifugation gets supernatant 800ml, and insoluble solid 90.5 gram (weight in wet base) supernatants are spray-dried that white powder finished product 70 restrains (moisture content 4.5%), and productive rate 80%(is in dry, down together) degree of hydrolysis 95%.
Example two
(moisture content 10% weighs 55 grams, with clear water rinsing 2-3 time, removes surface contaminants and down to get 5 in mao bird's nest.Place the hydrolyzer identical, add 600 milliliters in water, soak, boil, filter, weigh as method as described in the example one with example one, 95 milliliters of water cooking liquids, substrate 515 grams are adjusted the hydrolyzer temperature and are remained on 35-37 ℃, and substrate is packed in the hydrolyzer.Enzyme concentration by 200 units/gram takes by weighing 1398 neutral proteinases and makes enzymolysis liquid.Under agitation join equably in the hydrolyzer, clock.Hydrolysis then begins after 120 minutes to heat up, the PA-2 papain that when rising to 45 ℃, adds 5000 units/ml, continue hydrolysis, again after 60 minutes, filter as method as described in the example one, centrifugal and dry, get 420 milliliters of hydrolyzates, residue and down 21 grams (weight in wet base), insoluble solid 46.5 gram weight in wet bases) and off-white powder shape finished product 38 grams (moisture content 4.2%), productive rate 76.6%, degree of hydrolysis 96%.
Example three
Get waste material bird's nest slag (moisture content 5.8%) 100 grams, place hydrolyzer, add 1000 milliliters in water, and add 2 gram NaHCO
3Recording solution ph is 7.8, stirs to be warming up to 45 ℃, is warming up to after 90 minutes and boils, and boils, filters, weighs as method as described in the example one, claims to such an extent that substrate weighs 1014 grams.As method as described in the example one be hydrolyzed, filtration, centrifugal and dry, 1000 milliliters of filtrates, residue 159 grams (weight in wet base), 850 milliliters of supernatants, insoluble solid 70.1 grams (weight in wet base), buff powder finished product 47 grams (moisture content 4.8%).Degree of hydrolysis 84.3%, productive rate 56.3%.
Example four:
Get waste material bird's nest slag 100 grams, place hydrolyzer, add 1000 milliliters in water, and add 2.5 gram NaHCO
3(solution ph 8.2) stirs and to be warming up to 45 ℃, soaks to be warming up to after 120 minutes and boils, and boils, filters, weighs as method as described in the example one, claims to such an extent that substrate weighs 1100 and restrains.Adjust the hydrolyzer temperature and remain on 45 ℃ substrate reloaded in the hydrolyzer, take by weighing protease B.P and make 100 milliliters enzymolysis liquid with water cooking liquid by the enzyme dosage of 200 units/gram.Under agitation, join equably in the hydrolyzer, clock, and the observing response situation, hydrolysis adds the hydrochloric acid solution of 2N gradually after 90 minutes, be 2.0 until reactant PH, and so far most of substrate is hydrolyzed.Adjust the hydrolyzer temperature and keep 37 ℃, and add pepsin, continue hydrolysis by the dosage of 10000 units/gram, time is 90 minutes, centrifugal as filtration as described in the example one then, 920 milliliters of supernatants, residue 135 gram (weight in wet base) insoluble solids 39 grams (weight in wet base).In supernatant, add hydroxyl ion type strong-base anion-exchange resin (711 resins or 201 resins) neutralizing hydrolysis liquid at last to PH7 in batches, after 20 mesh filter screens are removed resin, spray-drying obtains yellow powder powder finished product 55.5 grams (moisture content 4.5%), degree of hydrolysis 87.7%, productive rate 64.1%.
In sum, the present invention has the following advantages:
1. therefore the down of the disposable processing bird's nest of the present invention's technology energy can significantly improve labor productivity.
2. the bird's nest made of the present invention's technology keeps original performance of bird's nest after testing.
3. to have optional proteolytic enzyme kind many for the present invention's technology, as adopting plant rennet, bacterialprotease, animal protease, reagent protease etc.
4. the present invention's technology can be processed the waste material in the existing processing-bird's nest slag, has improved utilization rate, and the rate of recovery reaches 50%, corresponding reducing cost, and can be used for processing former bird's nest, the half clean bird's nest adopted.
5. the bird's nest hydrolysis goods produced of the present invention's technology have good water-solublely, and high quality protein matter can directly be mixed with various products, such as additive or the nutrition fortifier of the pulvis goods that are used for different field, oral liquid, pill, high-grade cosmetics.
Claims (16)
1, a kind of hydrolysis processing method of bird's nest is characterized in that may further comprise the steps:
-the bird's nest raw material is placed in the hydrolyzer through rinsing repeatedly, soaked 60-120 minute down at 40 °-45 ℃;
-be warming up to boiling then, and keep boiling 20 minutes, the elimination water cooking liquid is standby, and bird's nest must wet;
-the bird's nest that will wet is put into hydrolyzer, regulates its pH value near neutrality or meta-alkalescence, stirs and heats up, and the control temperature is at constant temperature below 65 ℃;
-under constant temperature, directly add proteolytic enzyme or enzymolysis liquid, wherein the consumption of proteolytic enzyme is the 0.02-5.0% of wet bird's nest, is slowly keeping reaction time 1.5-3.5 hour under the stirring condition;
-after this, reactant liquor is warming up to boiling in 30 to 60 minutes gradually, and keep boiling 10 minutes;
-filter hydrolyzate, centrifugal through 10 minutes again, after the hydrolyzate drying is sprayed into powder.
2, a kind of the method for claim 1 is characterized in that said proteolytic enzyme is a papain.
3, a kind of the method for claim 1 is characterized in that said proteolytic enzyme is a bacterialprotease.
4, a kind of method as claimed in claim 3 is characterized in that described proteolytic enzyme is 1398 neutral proteinases.
5, a kind of the method for claim 1 is characterized in that described proteolytic enzyme is an animal protease.
6, a kind of method as claimed in claim 5 is characterized in that described proteolytic enzyme is a pepsin.
7, a kind of method as claimed in claim 5 is characterized in that described proteolytic enzyme is a trypsase.
8, a kind of the method for claim 1 is characterized in that said proteolytic enzyme is a reagent type protease.
9, a kind of method as claimed in claim 8 is characterized in that said proteolytic enzyme is protease B.P.
10, a kind of method as claimed in claim 8 is characterized in that said proteolytic enzyme is a Proteinase K.
11, a kind of the method for claim 1 is characterized in that the complex enzyme of said proteolytic enzyme for two or more.
12, a kind of the method for claim 1 is characterized in that said enzymolysis liquid made by water or water cooking liquid, and water or water cooking liquid consumption are no more than 20% of wet bird's nest.
13, a kind of bird's nest hydrolysis processing method is characterized in that may further comprise the steps:
-with the bird's nest raw material repeatedly rinsing be placed in the hydrolyzer, soaked 60-120 minute down at 40 °-45 ℃;
-be warming up to boiling then, and keep boiling 20 minutes, the elimination water cooking liquid is standby, and bird's nest must wet;
-the bird's nest that will wet is put into hydrolyzer, regulates its pH value near neutrality or meta-alkalescence, stirs and heats up, and the control temperature is at constant temperature below 65 ℃;
-under constant temperature, directly add proteolytic enzyme or enzymolysis liquid, wherein the consumption of proteolytic enzyme is the 0.02-5.0% of wet bird's nest, is slowly keeping reaction time 1.5-3.5 hour under the stirring condition.
-Jia acid stops the above-mentioned stage of reaction, adds corresponding acid protease afterwards again and continues reactant liquor is warming up to boiling in 30 to 60 minutes gradually, and keep boiling 10 minutes;
-filter hydrolyzate, centrifugal through 10 minutes again, after the hydrolyzate drying is sprayed into powder.
14,, it is characterized in that said raw material can be former bird's nest, half clean bird's nest or the bird's nest slag adopted as the described method of above-mentioned each claim.
15, the bird's nest hydrolysis goods of producing according to the described method of claim 1.
16, bird's nest hydrolysis goods according to claim 15 is characterized in that the additive or the nutrition fortifier of pulvis goods, oral liquid, pill, cosmetics.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN92112013A CN1085046A (en) | 1992-10-04 | 1992-10-04 | The enzymatic hydrolysis processing technology and the goods thereof of bird's nest |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN92112013A CN1085046A (en) | 1992-10-04 | 1992-10-04 | The enzymatic hydrolysis processing technology and the goods thereof of bird's nest |
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| CN1085046A true CN1085046A (en) | 1994-04-13 |
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| CN92112013A Pending CN1085046A (en) | 1992-10-04 | 1992-10-04 | The enzymatic hydrolysis processing technology and the goods thereof of bird's nest |
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Cited By (18)
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| CN102102119A (en) * | 2010-12-03 | 2011-06-22 | 北京中生奥普寡肽技术研究所 | Bird's nest oligopeptide composition and preparation method and use thereof |
| CN102771852A (en) * | 2011-12-31 | 2012-11-14 | 厦门市丝浓食品有限公司 | Sialic acid beverage and making method thereof |
| CN102907722A (en) * | 2012-01-04 | 2013-02-06 | 厦门市丝浓食品有限公司 | Cubilose juice drink and manufacturing method thereof |
| CN101757028B (en) * | 2008-12-22 | 2013-06-12 | 康贝株式会社 | Bone intensity improvement agent, skin collagen quantity improvement agent and oral composition and medicine part outer article containing at least one of the improvement agents |
| CN103436579A (en) * | 2013-08-01 | 2013-12-11 | 香港科技大学 | Method for preparing cubilose extract with strong antioxidation |
| CN104381554A (en) * | 2014-12-05 | 2015-03-04 | 燕之初颜如燕（厦门）生物科技有限公司 | Buccal tablet candies containing edible bird's nest components |
| CN104498572A (en) * | 2014-12-05 | 2015-04-08 | 燕之初颜如燕（厦门）生物科技有限公司 | Composite enzymolysis process for cubilose |
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| CN108371324A (en) * | 2018-01-18 | 2018-08-07 | 浙江燕宝丽堂电子商务有限公司 | A kind of preparation process of bird's nest essence oral solution |
| CN108902941A (en) * | 2018-06-28 | 2018-11-30 | 燕生（福建）生物工程有限公司 | A method of extracting Sialic acid from bird's nest |
| CN111265465A (en) * | 2020-04-01 | 2020-06-12 | 广州今盛美精细化工有限公司 | Cubilose extract and application thereof in preparation of cosmetics |
| CN111454810A (en) * | 2020-04-16 | 2020-07-28 | 林斌 | Cubilose white spirit and preparation method thereof |
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| CN113564218A (en) * | 2021-08-16 | 2021-10-29 | 厦门市燕之屋丝浓食品有限公司 | Whitening active small-molecular bird's nest peptide and preparation method thereof |
-
1992
- 1992-10-04 CN CN92112013A patent/CN1085046A/en active Pending
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| CN101757028B (en) * | 2008-12-22 | 2013-06-12 | 康贝株式会社 | Bone intensity improvement agent, skin collagen quantity improvement agent and oral composition and medicine part outer article containing at least one of the improvement agents |
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| CN102102119A (en) * | 2010-12-03 | 2011-06-22 | 北京中生奥普寡肽技术研究所 | Bird's nest oligopeptide composition and preparation method and use thereof |
| CN102102119B (en) * | 2010-12-03 | 2012-12-26 | 北京中生奥普寡肽技术研究所 | Bird's nest oligopeptide composition and preparation method and use thereof |
| CN102771852A (en) * | 2011-12-31 | 2012-11-14 | 厦门市丝浓食品有限公司 | Sialic acid beverage and making method thereof |
| CN102771852B (en) * | 2011-12-31 | 2013-12-18 | 厦门市丝浓食品有限公司 | Sialic acid beverage and making method thereof |
| CN102907722A (en) * | 2012-01-04 | 2013-02-06 | 厦门市丝浓食品有限公司 | Cubilose juice drink and manufacturing method thereof |
| CN103436579A (en) * | 2013-08-01 | 2013-12-11 | 香港科技大学 | Method for preparing cubilose extract with strong antioxidation |
| CN103436579B (en) * | 2013-08-01 | 2015-03-25 | 香港科技大学 | Method for preparing cubilose extract with strong antioxidation |
| CN104498572B (en) * | 2014-12-05 | 2017-10-27 | 燕之初颜如燕（厦门）生物科技有限公司 | Bird's nest complex enzyme hydrolysis processing technology |
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| CN106235303A (en) * | 2015-06-03 | 2016-12-21 | 莫智文 | For producing the acidic group enzymatic method of edible Nidus collocaliae extract |
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| CN107668312A (en) * | 2017-11-22 | 2018-02-09 | 福建农林大学 | A kind of integrated debubbling method of bird's nest protein liquid |
| CN107668312B (en) * | 2017-11-22 | 2020-12-29 | 福建农林大学 | Integrated defoaming method for cubilose protein liquid |
| CN108371324A (en) * | 2018-01-18 | 2018-08-07 | 浙江燕宝丽堂电子商务有限公司 | A kind of preparation process of bird's nest essence oral solution |
| CN108902941A (en) * | 2018-06-28 | 2018-11-30 | 燕生（福建）生物工程有限公司 | A method of extracting Sialic acid from bird's nest |
| CN111265465A (en) * | 2020-04-01 | 2020-06-12 | 广州今盛美精细化工有限公司 | Cubilose extract and application thereof in preparation of cosmetics |
| CN111454810A (en) * | 2020-04-16 | 2020-07-28 | 林斌 | Cubilose white spirit and preparation method thereof |
| CN112970931A (en) * | 2021-03-12 | 2021-06-18 | 长春大学 | Method for preparing digestion-free sea cubilose protein by using cubilose as raw material |
| CN113564218A (en) * | 2021-08-16 | 2021-10-29 | 厦门市燕之屋丝浓食品有限公司 | Whitening active small-molecular bird's nest peptide and preparation method thereof |
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