CN108500043A - A method of repairing chromium-polluted soil - Google Patents
A method of repairing chromium-polluted soil Download PDFInfo
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- CN108500043A CN108500043A CN201810280506.0A CN201810280506A CN108500043A CN 108500043 A CN108500043 A CN 108500043A CN 201810280506 A CN201810280506 A CN 201810280506A CN 108500043 A CN108500043 A CN 108500043A
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- soil
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
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- Microbiology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Soil Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Botany (AREA)
- Fertilizers (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention discloses a kind of methods for repairing chromium-polluted soil, include the following steps：（1）Prepare fertilizer：Raw material includes 35 43 parts by weight of organic fertilizer, 13 15 parts by weight of zeolite, 0.2 0.6 parts by weight of iron powder, 14 18 parts by weight of plant ash, 22 26 parts by weight of quick lime；（2）Prepare bacteria suspension；（3）Soil moisture is maintained at 32 40% after loosening the soil, and the soil moisture is maintained at 20 30 DEG C；42 44kg/ mus of step is added in the soil（1）In the fertilizer that is prepared, dig up and down；（4）By step（2）The bacteria suspension of middle preparation is uniformly sprayed at the soil surface, and fountain height is 1.6 2.0kg/ mus；（5）In step（4）In soil in be sprinkled into the Amaranthus retroflexus seed disinfected.The method that chromium-polluted soil is repaired in the present invention significantly improves Amaranthus retroflexus to chromium biological concentration concentration and remediation efficiency, good to chromium-polluted soil repairing effect.
Description
Technical field
The present invention relates to heavy-metal contaminated soil Treatment process fields, and in particular to a kind of side for repairing chromium-polluted soil
Method.
Background technology
With industry, municipal pollution aggravation, agrochemicals type and quantity increase significantly and Wastewater Irrigation in Farmland, change
The excessively unreasonable mankind's activity such as application of fertile pesticide results in heavy metal in soil pollution getting worse, in turn results in ecology
Destroy the phenomenon that deteriorating with environmental quality.According to 2014《National Soil Pollution Investigation bulletin》It has been shown that, 8 kinds of inorganic pollutions
Point exceeding standard rate be respectively：Cadmium（7.0%）, lead（1.5%）, mercury（1.6%）, arsenic（2.7%）, copper（2.1%）, chromium（1.1%）, zinc
（0.9%）, nickel（4.8%）.
Pollution of chromium come from chrome ore exploitation, smelt, and containing chromium compound pigment, alloy, plating, tanning, offset printing,
Printing and dyeing and application agriculturally.The unprocessed inflow river of chromate waste water, chromium slag stack field and fail to comply fully with " ne-leakage, nothing
Scatter, without be lost in " requirement, so that a large amount of Cr VIs, trivalent chromium and chromium compound is entered soil and pollute.
The existing method remediation efficiency by microorganism-phytoremediation chromium-polluted soil is not high, the chromium tolerance of microorganism
Property it is not high, can not be used in high chromium concentration contaminated soil, and using plant chromium concn and transfer ratio it is not high.
Invention content
The present invention provides a kind of methods for repairing chromium-polluted soil to solve problem of the prior art.
The purpose of the present invention is what is realized with following technical proposals：
A method of chromium-polluted soil is repaired, is included the following steps：
（1）Prepare fertilizer：Raw material include organic fertilizer 35-43 parts by weight, zeolite 13-15 parts by weight, iron powder 0.2-0.6 parts by weight,
Plant ash 14-18 parts by weight, quick lime 22-26 parts by weight；
（2）Prepare bacteria suspension；
（3）Soil moisture is maintained at 32-40% after loosening the soil, and the soil moisture is maintained at 20-30 DEG C；42- is added in the soil
44kg/ mus of steps（1）In the fertilizer that is prepared, dig up and down；
（4）By step（2）The bacteria suspension of middle preparation is uniformly sprayed at the soil surface, and fountain height is 1.6-2.0kg/ mus；
（5）In step（4）In soil in be sprinkled into the Amaranthus retroflexus seed disinfected, by Amaranthus retroflexus to the absorption of chromium and described
Chromium in the invigoration effect combined removal soil of bacteria suspension and fertilizer.
Preferably, the bacteria suspension is made by the steps and obtains：
（1）Separate pseudomonas putida；
（2）Take beancake powder 15-18.0g, CaCO34-6g, glucose 32-36g, KNO30.8-1.2g, K2HPO40.5-0.7g,
MgSO4·7H2O0.6-0.8g, NaCl1-2g, agar 12-15g, distilled water 1000ml, pH adjust to 7.1-7.2 after in 118-
122 °C of sterilizing 25min prepare culture medium；
（3）By step（1）In the pseudomonas putida isolated be seeded to step（2）In the culture medium of middle preparation；
（4）By step（3）The middle culture medium for being inoculated with pseudomonas putida is in 30 DEG C, 150rmin-1Under the conditions of cultivate for 24 hours,
8000rpm centrifuges 8min and collects Pseudomonas putida thalline, and pseudomonas putida weight is suspended from sterile water after cleaning and obtains bacterium
Suspension, OD600=1.0。
Beneficial effects of the present invention are as follows：
1, the present invention is used to prepare bacteria suspension using pseudomonas putida, and the chromium tolerance of pseudomonas putida is strong, has very strong
Purpose and specific aim.
2, the present invention improves the absorption of chromium with bacteria suspension made from rational method and fertilizer Amaranthus retroflexus anti-
The chromium concn of branch amaranth aerial part and under ground portion, the gross accumulation amount of the chromium of aerial part increase 14.0%-79.5%, underground part
The gross accumulation amount of the chromium divided increases 8.0%-58.2%.The method that chromium-polluted soil is repaired in the present invention significantly improves Amaranthus retroflexus
It is good to chromium-polluted soil repairing effect to chromium biological concentration concentration and remediation efficiency.
Specific implementation mode
Embodiment 1
A method of chromium-polluted soil is repaired, is included the following steps：
（1）Prepare fertilizer：Raw material include organic fertilizer 35-43 parts by weight, zeolite 13-15 parts by weight, iron powder 0.2-0.6 parts by weight,
Plant ash 14-18 parts by weight, quick lime 22-26 parts by weight；
（2）Prepare bacteria suspension；
（3）Soil moisture is maintained at 32-40% after loosening the soil, and the soil moisture is maintained at 20-30 DEG C；42- is added in the soil
44kg/ mus of steps（1）In the fertilizer that is prepared, dig up and down；
（4）By step（2）The bacteria suspension of middle preparation is uniformly sprayed at the soil surface, and fountain height is 1.6-2.0kg/ mus；
（5）In step（4）In soil in be sprinkled into the Amaranthus retroflexus seed disinfected, by Amaranthus retroflexus to the absorption of chromium and described
Chromium in the invigoration effect combined removal soil of bacteria suspension and fertilizer.
Embodiment 2
A method of chromium-polluted soil is repaired, is included the following steps：
（1）Prepare fertilizer：Raw material include organic fertilizer 35-43 parts by weight, zeolite 13-15 parts by weight, iron powder 0.2-0.6 parts by weight,
Plant ash 14-18 parts by weight, quick lime 22-26 parts by weight；
（2）Prepare bacteria suspension:
A. pseudomonas putida is separated；
B. beancake powder 15-18.0g, CaCO are taken34-6g, glucose 32-36g, KNO30.8-1.2g, K2HPO40.5-0.7g,
MgSO4·7H2O0.6-0.8g, NaCl1-2g, agar 12-15g, distilled water 1000ml, pH adjust to 7.1-7.2 after in 118-
122 °C of sterilizing 25min prepare culture medium；
C. the pseudomonas putida isolated in step a is seeded in the culture medium prepared in step b；
D. the culture medium of pseudomonas putida will be inoculated in step c in 30 DEG C, 150rmin-1Under the conditions of cultivate for 24 hours,
8000rpm centrifuges 8min and collects Pseudomonas putida thalline, and pseudomonas putida weight is suspended from sterile water after cleaning and obtains bacterium
Suspension, OD600=1.0。
（3）Soil moisture is maintained at 32-40% after loosening the soil, and the soil moisture is maintained at 20-30 DEG C；It is added in the soil
42-44kg/ mus of steps（1）In the fertilizer that is prepared, dig up and down；
（4）By step（2）The bacteria suspension of middle preparation is uniformly sprayed at the soil surface, and fountain height is 1.6-2.0kg/ mus；
（5）In step（4）In soil in be sprinkled into the Amaranthus retroflexus seed disinfected, by Amaranthus retroflexus to the absorption of chromium and described
Chromium in the invigoration effect combined removal soil of bacteria suspension and fertilizer.
Comparative example 1
A method of chromium-polluted soil is repaired, is included the following steps：
（1）Prepare fertilizer：Raw material include organic fertilizer 35-43 parts by weight, zeolite 13-15 parts by weight, iron powder 0.2-0.6 parts by weight,
Plant ash 14-18 parts by weight, quick lime 22-26 parts by weight；
（2）Soil moisture is maintained at 32-40% after loosening the soil, and the soil moisture is maintained at 20-30 DEG C；42- is added in the soil
44kg/ mus of steps（1）In the fertilizer that is prepared, dig up and down；
（3）In step（2）In soil in be sprinkled into the Amaranthus retroflexus seed disinfected, by Amaranthus retroflexus to the absorption of chromium and described
Chromium in the invigoration effect combined removal soil of fertilizer.
Comparative example 2
A method of chromium-polluted soil is repaired, is included the following steps：
（1）Prepare bacteria suspension:
A. pseudomonas putida is separated；
B. beancake powder 15-18.0g, CaCO are taken34-6g, glucose 32-36g, KNO30.8-1.2g, K2HPO40.5-0.7g,
MgSO4·7H2O0.6-0.8g, NaCl1-2g, agar 12-15g, distilled water 1000ml, pH adjust to 7.1-7.2 after in 118-
122 °C of sterilizing 25min prepare culture medium；
C. the pseudomonas putida isolated in step a is seeded in the culture medium prepared in step b；
D. the culture medium of pseudomonas putida will be inoculated in step c in 30 DEG C, 150rmin-1Under the conditions of cultivate for 24 hours,
8000rpm centrifuges 8min and collects Pseudomonas putida thalline, and pseudomonas putida weight is suspended from sterile water after cleaning and obtains bacterium
Suspension, OD600=1.0。
（2）Soil moisture is maintained at 32-40% after loosening the soil, and the soil moisture is maintained at 20-30 DEG C；It is equal in the soil surface
Even sprinkling step（1）The bacteria suspension of middle preparation, fountain height are 1.6-2.0kg/ mus；
（3）In step（2）In soil in be sprinkled into the Amaranthus retroflexus seed disinfected, by Amaranthus retroflexus to the absorption of chromium and described
Chromium in the invigoration effect combined removal soil of bacteria suspension.
1, the influence of Amaranthus retroflexus growth, tolerance and chromium accumulation is tested in chromium-polluted soil
Experiment sets 4 processing：
CK：The Amaranthus retroflexus seed disinfected is sprinkled into chromium-polluted soil；
A：Use the method reparation of the reparation chromium-polluted soil in embodiment 2；
B：Use the method reparation of the reparation chromium-polluted soil in comparative example 1；
C：Use the method reparation of the reparation chromium-polluted soil in comparative example 2.
Above-mentioned 4 processing are respectively 0,100mgkg in chromium concn-1、300mg·kg-1And 500mgkg-1Humidity be
It is repaired according to the restorative procedure of the reparation chromium-polluted soil in 4 processing in 30% soil, the Amaranthus retroflexus growth stage is 90
It, plant height, root long, ground dry weight and underground dry weight are as shown in the table.
1 different disposal of table influences tables of data to Amaranthus retroflexus plant height（Unit：cm）
| Chromium concn in soil | 0 | 100mg·kg-1 | 300mg·kg-1 | 500mg·kg-1 |
| CK processing | 28 | 27 | 26 | 24 |
| A processing | 35 | 34 | 34 | 33 |
| B processing | 33 | 31 | 28 | 26 |
| C processing | 29 | 28 | 27 | 27 |
It can be seen that from upper table data, the plant height highest of A processing, is respectively 0,100mgkg in soil chromium concn-1、
300mg·kg-1And 500mgkg-1When, respectively 25%, 25.9%, 30.8% and 37.5% is significantly increased compared with control.
2 different disposal of table influences tables of data to Amaranthus retroflexus chlorophyll content（Unit：mg·g-1）
| Chromium concn in soil | 0 | 100mg·kg-1 | 300mg·kg-1 | 500mg·kg-1 |
| CK processing | 1.3 | 1.1 | 1.0 | 0.8 |
| A processing | 1.4 | 1.3 | 1.3 | 1.0 |
| B processing | 1.4 | 1.2 | 1.2 | 1.0 |
| C processing | 1.3 | 1.2 | 1.1 | 0.9 |
It can be seen that from upper table data, the chlorophyll content highest of A processing, is respectively 0,100mgkg in soil chromium concn-1、
300mg·kg-1And 500mgkg-1When, respectively 7.7%, 18.2%, 30.0% and 25.0% is significantly increased compared with control.
3 different disposal of table influences tables of data to Amaranthus retroflexus aerial part chromium concn（Unit：mg·kg-1）
| Chromium concn in soil | 100mg·kg-1 | 300mg·kg-1 | 500mg·kg-1 |
| CK processing | 547 | 1037 | 1154 |
| A processing | 982 | 1247 | 1315 |
| B processing | 598 | 1042 | 1162 |
| C processing | 768 | 1045 | 1167 |
It can be seen that from upper table data, the Amaranthus retroflexus aerial part chromium concn highest of A processing is respectively in soil chromium concn
100mg·kg-1、300mg·kg-1And 500mgkg-1When, respectively 79.5%, 20.3% and 14.0% is significantly increased compared with control.
4 different disposal of table influences tables of data to Amaranthus retroflexus under ground portion chromium concn（Unit：mg·kg-1）
| Chromium concn in soil | 100mg·kg-1 | 300mg·kg-1 | 500mg·kg-1 |
| CK processing | 1165 | 2046 | 2267 |
| A processing | 1843 | 2369 | 2449 |
| B processing | 1192 | 2156 | 2287 |
| C processing | 1564 | 2196 | 2354 |
It can be seen that from upper table data, the Amaranthus retroflexus under ground portion chromium concn highest of A processing is respectively in soil chromium concn
100mg·kg-1、300mg·kg-1And 500mgkg-1When, respectively 58.2%, 15.8% and 8.0% is significantly increased compared with control.
Claims (2)
1. a kind of method for repairing chromium-polluted soil, it is characterised in that include the following steps：
（1）Prepare fertilizer：Raw material include organic fertilizer 35-43 parts by weight, zeolite 13-15 parts by weight, iron powder 0.2-0.6 parts by weight,
Plant ash 14-18 parts by weight, quick lime 22-26 parts by weight；
（2）Prepare bacteria suspension；
（3）Soil moisture is maintained at 32-40% after loosening the soil, and the soil moisture is maintained at 20-30 DEG C；42- is added in the soil
44kg/ mus of steps（1）In the fertilizer that is prepared, dig up and down；
（4）By step（2）The bacteria suspension of middle preparation is uniformly sprayed at the soil surface, and fountain height is 1.6-2.0kg/ mus；
（5）In step（4）In soil in be sprinkled into the Amaranthus retroflexus seed disinfected, by Amaranthus retroflexus to the absorption of chromium and described
Chromium in the invigoration effect combined removal soil of bacteria suspension and fertilizer.
2. a kind of method for repairing chromium-polluted soil according to claim 1, it is characterised in that the bacteria suspension passes through such as
Lower step is prepared：
（1）Separate pseudomonas putida；
（2）Take beancake powder 15-18.0g, CaCO34-6g, glucose 32-36g, KNO30.8-1.2g, K2HPO40.5-0.7g,
MgSO4·7H2O0.6-0.8g, NaCl1-2g, agar 12-15g, distilled water 1000ml, pH adjust to 7.1-7.2 after in 118-
122 °C of sterilizing 25min prepare culture medium；
（3）By step（1）In the pseudomonas putida isolated be seeded to step（2）In the culture medium of middle preparation；
（4）By step（3）The middle culture medium for being inoculated with pseudomonas putida is in 30 DEG C, 150rmin-1Under the conditions of cultivate for 24 hours,
8000rpm centrifuges 8min and collects Pseudomonas putida thalline, and pseudomonas putida weight is suspended from sterile water after cleaning and obtains bacterium
Suspension, OD600=1.0。
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810280506.0A CN108500043A (en) | 2018-04-02 | 2018-04-02 | A method of repairing chromium-polluted soil |
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| CN201810280506.0A CN108500043A (en) | 2018-04-02 | 2018-04-02 | A method of repairing chromium-polluted soil |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105170641A (en) * | 2015-09-30 | 2015-12-23 | 河南行知专利服务有限公司 | Combined remediation method for heavy metal contaminated soil |
| CN106583430A (en) * | 2016-12-09 | 2017-04-26 | 北京高能时代环境技术股份有限公司 | Method for repairing arsenium-polluted farmland soil through microorganisms and plants in combined manner |
| CN106754582A (en) * | 2017-03-21 | 2017-05-31 | 青岛农业大学 | Pseudomonas putida RXX 01 and its application in soil phthalic acid ester of degrading |
| CN107377615A (en) * | 2017-07-31 | 2017-11-24 | 郭婷 | A kind of simple, soil remediation method for easily implementing |
-
2018
- 2018-04-02 CN CN201810280506.0A patent/CN108500043A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105170641A (en) * | 2015-09-30 | 2015-12-23 | 河南行知专利服务有限公司 | Combined remediation method for heavy metal contaminated soil |
| CN106583430A (en) * | 2016-12-09 | 2017-04-26 | 北京高能时代环境技术股份有限公司 | Method for repairing arsenium-polluted farmland soil through microorganisms and plants in combined manner |
| CN106754582A (en) * | 2017-03-21 | 2017-05-31 | 青岛农业大学 | Pseudomonas putida RXX 01 and its application in soil phthalic acid ester of degrading |
| CN107377615A (en) * | 2017-07-31 | 2017-11-24 | 郭婷 | A kind of simple, soil remediation method for easily implementing |
Non-Patent Citations (2)
| Title |
|---|
| 朱伟萍等: "《水处理生物学》", 31 August 2008, 中国电力出版社 * |
| 郑施雯等: "土壤铬污染修复技术研究概述", 《河南科技》 * |
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Application publication date: 20180907 |