CN108478597B - Polydopamine-modified molybdenum disulfide composite silver nanoparticle antibacterial agent and preparation method thereof - Google Patents
Polydopamine-modified molybdenum disulfide composite silver nanoparticle antibacterial agent and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a preparation method of a polydopamine-modified molybdenum disulfide composite silver nanoparticle antibacterial agent, which comprises the following steps: preparing a molybdenum disulfide @ polydopamine nanosheet solution; preparing a silver nitrate solution, measuring ammonia water, wherein the molar ratio of the ammonia water to the silver nitrate is 50-100: 1, the molar ratio of silver nitrate to molybdenum disulfide is 1-50: 1; preparing a glucose solution, wherein the molar ratio of glucose to silver nitrate is 10: 1; adding the solution prepared in the first two steps and ammonia water into a wide-mouth bottle, magnetically stirring for 20min to 30min, adding a glucose solution, magnetically stirring for 1h to 1.5h or reacting for 10min at 60 ℃ by microwave for reduction; the product was washed with ultrapure water, centrifuged to purify the product, and the final product was dispersed in ultrapure water and stored in a refrigerator at 4 ℃. The preparation method is simple and efficient, has good reproducibility, is convenient for batch production, and meets the environment-friendly requirement; the polydopamine-modified molybdenum disulfide composite silver nanoparticle antibacterial agent prepared by the method is regular in appearance, uniform in particle size, uniform in dispersion, low in cost and good in antibacterial effect.
Description
Technical Field
The invention belongs to the technical field of synthesis of nano materials, and particularly relates to a novel polydopamine-modified molybdenum disulfide composite silver nanoparticle antibacterial agent and a preparation method thereof.
Background
Bacterial infections continue to be a serious threat to human health, and increasingly severe bacterial resistance makes bacterial infections increasingly difficult to combat. Research has shown that bacterial biofilms are one of the important mechanisms for the development of bacterial drug resistance, and are the main causes of recurrent outbreaks and difficult control of many chronic infectious diseases. Antibiotics represented by penicillin have been the main treatment means for bacterial infectious diseases. Traditional antibacterial therapies rely on antibiotics to kill or interfere with bacterial growth, and their use is limited by toxicity and allergic reactions to host cells. In addition, frequent use of antibiotics leads to multidrug resistance. In recent years, a large number of novel drug-resistant strains emerge due to antibiotic abuse, the bacterial drug resistance is stronger and stronger, and the defects of large toxic and side effects, high cost and the like of the traditional treatment strategy depending on antibiotics are more obvious. On the other hand, the development of new antibiotics is becoming more and more difficult, and not only is the research and development cost high, but also the variety is extremely limited. Therefore, there is an urgent need to develop new and effective antibacterial agents to overcome the disadvantages of conventional antibiotics.
With the rapid development of nano science and technology in recent years, a large number of novel nano materials are continuously emerged, and good application potential is shown in the antibacterial field. Among them, silver nanoparticles (Ag NPs) have been proven to be an effective nano antibacterial agent having a unique biochemical antibacterial mechanism, but have problems of easy aggregation, insufficient stability, and the like. Molybdenum disulfide (MoS)2) Is a novel two-dimensional nano material, has extremely high specific surface area and unique chemical and physical properties, and can be used as a drug controlled release carrier. Through the efficient loading and the stability of the molybdenum disulfide nanosheet, the problems of silver nanoparticles can be effectively solved. However, molybdenum disulfide is easy to agglomerate in physiological environment, and surface defects are few, so that the biological functionalization is not facilitated.
Disclosure of Invention
Aiming at the defects or improvement requirements of the prior art, the invention provides a polydopamine modified molybdenum disulfide composite silver nanoparticle antibacterial agent and a preparation method thereof.
In order to achieve the above object, according to one aspect of the present invention, there is provided a method for preparing a polydopamine-modified molybdenum disulfide composite silver nanoparticle antibacterial agent, comprising the steps of:
(1) molybdenum disulfide (MoS) with Polydopamine (PDA)2) Surface modification is carried out to prepare molybdenum disulfide @ polydopamine (MoS)2@ PDA) nanosheets;
(2) preparing a silver nitrate transparent solution, and measuring ammonia water, wherein the molar ratio of the ammonia water to the silver nitrate is 50-100: 1, the molar ratio of silver nitrate to molybdenum disulfide is 1-50: 1;
(3) preparing a reducing agent glucose solution, wherein the molar ratio of glucose to silver nitrate is 10: 1;
(4) adding the solution obtained in the step (1) and the solution obtained in the step (2) into a wide-mouth bottle, magnetically stirring for 20-30 min for mixing, adding the glucose solution prepared in the step (3), and reducing by adopting one of a magnetic stirring mode for 1-1.5 h and a microwave reaction mode at 60 ℃ for 10 min;
(5) and (3) cleaning the product obtained in the step (4) by using ultrapure water, and then carrying out centrifugal purification, wherein the obtained final product is dispersed in the ultrapure water and stored in a refrigerator at 4 ℃.
Further, in the step (1), the preparation method of the molybdenum disulfide @ polydopamine nanosheet is as follows:
a. respectively preparing a dopamine transparent solution and a molybdenum disulfide solution, wherein the molar ratio of dopamine to molybdenum disulfide is 5: 1;
b. preparing 20mL of Tris-HCl buffer solution of triaminomethane, wherein the concentration is 10mM and the pH value is 8.5;
c. and (b) adding the dopamine solution and the molybdenum disulfide solution in the step a into the Tris-HCl buffer solution in the step b, magnetically stirring for 5min for uniform mixing, transferring the mixed solution into a 35mL special microwave tube, and then placing the special microwave tube on a microwave reactor for heating for 10min, wherein the microwave power is 100W, the pressure is 150PSI, and the heating temperature is 60 ℃.
Further, in the step c, a water bath ultrasonic reaction is adopted, and the method comprises the following steps:
transferring the solutions prepared in the steps a and b into a wide-mouth bottle for mixing, and then placing the wide-mouth bottle into a water bath ultrasonic instrument for water bath ultrasonic reaction, wherein the ultrasonic power is 53KHz, the ultrasonic time is 1-6 h, and the ultrasonic temperature is 20-40 ℃;
further, in the step (2), the concentration of the ammonia water is 25% -28%.
Further, in the step (5), the centrifugal rotating speed is 8000rpm-12000rpm, and the centrifugal time is 20 min-40 min.
According to another aspect of the invention, a polydopamine-modified molybdenum disulfide composite silver nanoparticle antibacterial agent is provided, and the polydopamine-modified molybdenum disulfide composite silver nanoparticle antibacterial agent is prepared by the preparation method.
Compared with the prior art, the technical scheme of the invention has the following advantages:
1. the preparation method is simple, mild, efficient, good in reproducibility, convenient for batch production and in line with the environment-friendly requirement.
2. The polydopamine modified molybdenum disulfide composite silver nanoparticle antibacterial agent prepared by the preparation method provided by the invention is regular in appearance, uniform in silver nanoparticle size, uniform in dispersion, low in material price, low in cost and good in antibacterial effect.
Drawings
Fig. 1 is a Transmission Electron Microscope (TEM) image of the polydopamine modified molybdenum disulfide composite silver nanoparticle antibacterial agent obtained in example 1 of the present invention.
Fig. 2 is an Atomic Force Microscope (AFM) image of the polydopamine modified molybdenum disulfide composite silver nanoparticle antibacterial agent obtained in example 1 of the present invention.
Fig. 3 is a Transmission Electron Microscope (TEM) image of the polydopamine modified molybdenum disulfide composite silver nanoparticle antibacterial agent obtained in example 2 of the present invention.
FIG. 4 is a Transmission Electron Microscope (TEM) image of the polydopamine modified molybdenum disulfide composite silver nanoparticle antibacterial agent obtained in example 3 of the present invention.
FIG. 5 is a Transmission Electron Micrograph (TEM) of the polydopamine modified molybdenum disulfide composite silver nanoparticle antibacterial agent obtained in example 4 of the present invention;
FIG. 6 is a Transmission Electron Microscope (TEM) image of the polydopamine modified molybdenum disulfide composite silver nanoparticle antibacterial agent obtained in example 5 of the present invention.
Fig. 7 is a high-resolution transmission electron micrograph (HRTEM) of the polydopamine-modified molybdenum disulfide composite silver nanoparticle antibacterial agent obtained in examples 1, 2, 3, 4 and 5 of the present invention.
FIG. 8 is a graph showing the effect of the polydopamine-modified molybdenum disulfide composite silver nanoparticle antibacterial agent obtained in example 1 of the present invention on inhibiting Escherichia coli.
Fig. 9 is a graph showing the effect of the polydopamine-modified molybdenum disulfide composite silver nanoparticle antibacterial agent on inhibiting staphylococcus aureus, obtained in example 1 of the present invention.
Fig. 10 and fig. 11 are a Transmission Electron Microscope (TEM) image and an Atomic Force Microscope (AFM) image of the polydopamine modified molybdenum disulfide nanosheet obtained in example a of the present invention, respectively.
Fig. 12 and 13 are a Transmission Electron Microscope (TEM) image and an Atomic Force Microscope (AFM) image of the polydopamine-modified molybdenum disulfide nanosheet obtained in example B of the present invention, respectively.
Fig. 14 and 15 are a Transmission Electron Microscope (TEM) image and an Atomic Force Microscope (AFM) image of the polydopamine-modified molybdenum disulfide nanosheet obtained in example C of the present invention, respectively.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments. Examples of the embodiments are illustrated in the accompanying drawings, and specific embodiments described in the following embodiments of the invention are provided as illustrative of the embodiments of the invention only and are not intended to be limiting of the invention.
Example 1
0.8mL of 10mg/mL silver nitrate solution, 5mL of 150. mu.g/mL molybdenum disulfide @ polydopamine (MoS)2@ PDA) nanosheet solution was added to a 50mL jar containing 90. mu.L of aqueous ammonia solution and magnetically stirred for 30 min. Then 9.3mL of 10mg/mL glucose solution was added and the reaction was continued with magnetic stirring for 1.5 h. Washing with ultrapure water for many times, wherein the centrifugal rotation speed is 12000rpm, the centrifugal time is 40min, and dispersing the final product in the ultrapure water to obtain the polydopamine-modified molybdenum disulfide composite silver nanoparticle antibacterial agent. Polydopamine modified molybdenum disulfide composite silver nanoparticles in figure 1The granular antibacterial agent has regular appearance and uniform dispersion, and the size of the silver nano-particles is about 5-6 nm. From figure 2 it can be seen that the thickness of the prepared polydopamine modified molybdenum disulfide composite silver nanoparticle antibacterial agent is about 10 nm. Under the condition, the molar ratio of ammonia water to silver nitrate is 50: 1, the molar ratio of silver nitrate to molybdenum disulfide is 10: 1.
example 2
0.8mL of 10mg/mL silver nitrate solution, 5mL of 150. mu.g/mL molybdenum disulfide @ polydopamine (MoS)2@ PDA) nanosheet solution was added to a glass vessel containing 180. mu.L of ammonia solution and magnetically stirred for 20 min. Then 9.3mL of 10mg/mL glucose solution was added and the reaction was continued with magnetic stirring for 1 h. Washing with ultrapure water for many times, wherein the centrifugal rotation speed is 12000rpm, the centrifugal time is 20min, and dispersing the final product in the ultrapure water to obtain the polydopamine-modified molybdenum disulfide composite silver nanoparticle antibacterial agent. In the figure 3, the poly-dopamine modified molybdenum disulfide composite silver nanoparticle antibacterial agent has regular appearance, dense silver nanoparticle distribution and about 5-6nm size. Under the condition, the molar ratio of ammonia water to silver nitrate is 100: 1, the molar ratio of silver nitrate to molybdenum disulfide is 10: 1.
example 3
0.8mL of 10mg/mL silver nitrate solution, 5mL of 150. mu.g/mL molybdenum disulfide @ polydopamine (MoS)2@ PDA) nanosheet solution was added to a glass vessel containing 180. mu.L of ammonia solution and magnetically stirred for 20 min. Then, 9.3mL of a 10mg/mL glucose solution was added and the reaction was carried out by microwave at 60 ℃ for 10 min. Washing with ultrapure water for many times, wherein the centrifugal rotation speed is 12000rpm, the centrifugal time is 20min, and dispersing the final product in the ultrapure water to obtain the polydopamine-modified molybdenum disulfide composite silver nanoparticle antibacterial agent. In FIG. 4, the poly-dopamine modified molybdenum disulfide composite silver nanoparticle antibacterial agent has regular appearance, dense silver nanoparticle distribution and about 5-6nm size. Under the condition, the molar ratio of ammonia water to silver nitrate is 100: 1, the molar ratio of silver nitrate to molybdenum disulfide is 10: 1.
example 4
80 μ L of 10mg/mL silver nitrate solution, 5mL of 150 μ g/mL molybdenum disulfide @ polydopamine (MoS)2@ PDA) nanosheet solution containing18 μ L of ammonia solution in a glass vessel, magnetically stir for 20 min. Then 0.93mL of 10mg/mL glucose solution was added and the reaction was continued with magnetic stirring for 1 h. Washing with ultrapure water for many times, wherein the centrifugal speed is 8000rpm, the centrifugal time is 30min, and dispersing the final product in the ultrapure water to obtain the polydopamine-modified molybdenum disulfide composite silver nanoparticle antibacterial agent. In the figure 5, the poly-dopamine modified molybdenum disulfide composite silver nanoparticle antibacterial agent has regular appearance, and the size of the silver nanoparticle is about 5-6 nm. Under the condition, the molar ratio of ammonia water to silver nitrate is 100: 1, the molar ratio of silver nitrate to molybdenum disulfide is 1: 1.
example 5
4mL of 10mg/mL silver nitrate solution, 5mL of 150. mu.g/mL molybdenum disulfide @ polydopamine (MoS)2@ PDA) the nanosheet solution was added to a glassware containing 900. mu.L of aqueous ammonia solution and magnetically stirred for 30 min. Then 4.65mL of 100mg/mL glucose solution was added and the reaction was continued with magnetic stirring for 1.5 h. Washing with ultrapure water for many times, wherein the centrifugal rotation speed is 12000rpm, the centrifugal time is 40min, and dispersing the final product in the ultrapure water to obtain the polydopamine-modified molybdenum disulfide composite silver nanoparticle antibacterial agent. In FIG. 6, the appearance of the polydopamine modified molybdenum disulfide composite silver nanoparticle antibacterial agent is regular, and the size of the silver nanoparticle is about 5-6 nm. Under the condition, the molar ratio of ammonia water to silver nitrate is 100: 1, the molar ratio of silver nitrate to molybdenum disulfide is 50: 1.
in addition, the invention provides three applications of Polydopamine (PDA) to molybdenum disulfide (MoS)2) Surface modification to prepare molybdenum disulfide @ polydopamine (MoS)2@ PDA) nanoplates.
Example A
mu.L of 10mg/mL dopamine solution, 2mL of 0.1mg/mL molybdenum disulfide solution was added to a 50mL jar containing 20mL Tris-HCl buffer solution and sonicated in a water bath at 40 ℃ for 1 h. Washing with ultrapure water for many times, wherein the centrifugal speed is 8000rpm, the centrifugal time is 40min, and dispersing the final product in the ultrapure water to obtain the polydopamine-modified molybdenum disulfide nanosheet. In the figure 10, the polydopamine modified molybdenum disulfide nanosheet is regular in shape and uniform in dispersion, and the size of the nanosheet is about 200nm-400 nm. From fig. 11, it can be seen that the thickness of the prepared polydopamine modified molybdenum disulfide nanosheet is about 5 nm.
Example B
mu.L of 10mg/mL dopamine solution, 2mL of 0.1mg/mL molybdenum disulfide solution was added to a 50mL jar containing 20mL Tris-HCl buffer solution and sonicated in a water bath at 30 ℃ for 3 h. And washing with ultrapure water for multiple times, wherein the centrifugal rotation speed is 12000rpm, the centrifugal time is 30min, and dispersing the final product in the ultrapure water to obtain the polydopamine-modified molybdenum disulfide nanosheet. In FIG. 12, the polydopamine modified molybdenum disulfide nanosheets are regular in morphology and uniform in dispersion, and the size of the nanosheets is about 200nm-450 nm. From fig. 13, it can be seen that the thickness of the prepared polydopamine modified molybdenum disulfide nanosheet is about 6 nm.
Example C
mu.L of 10mg/mL dopamine solution, 2mL of 0.1mg/mL molybdenum disulfide solution was added to a 50mL jar containing 20mL Tris-HCl buffer solution and sonicated in a water bath at 20 ℃ for 6 h. And washing with ultrapure water for multiple times, wherein the centrifugal rotation speed is 12000rpm, the centrifugal time is 30min, and dispersing the final product in the ultrapure water to obtain the polydopamine-modified molybdenum disulfide nanosheet. In the figure 14, the polydopamine modified molybdenum disulfide nanosheet is regular in shape and uniform in dispersion, and the size of the nanosheet is about 250nm-400 nm. From fig. 15, it can be seen that the thickness of the prepared polydopamine modified molybdenum disulfide nanosheet is about 6.5 nm.
The polydopamine-modified molybdenum disulfide composite silver nanoparticle antibacterial agent prepared by the preparation method has 99% inhibition rate on escherichia coli at 25 mu g/mL and 99% inhibition rate on staphylococcus aureus at 125 mu g/mL.
The poly-dopamine (PDA) adopted by the invention is an environment-friendly biological macromolecule, and can be adhered to the surfaces of most of organic and inorganic substances (such as polymers, proteins and the like) to form uniform coating layers through dopamine oxidative self-polymerization; the polydopamine has good stability and improves the hydrophilicity, colloidal stability and biocompatibility of the nano material.
According to the invention, polydopamine with excellent adhesion and biocompatibility is adopted to carry out surface modification on the molybdenum disulfide nanosheets, so that the antibacterial material of the polydopamine-modified molybdenum disulfide composite silver nanoparticle is prepared, and the stability problems of the molybdenum disulfide nanosheets and the silver nanoparticles are effectively solved at the same time.
The invention provides a simple and efficient preparation method of a polydopamine-modified molybdenum disulfide composite silver nanoparticle antibacterial agent, which is simple, mild, efficient and good in reproducibility, is convenient for batch production, and meets the requirement of environmental friendliness. Meanwhile, the polydopamine modified molybdenum disulfide composite silver nanoparticle antibacterial agent prepared by the preparation method provided by the invention is regular in appearance, uniform in silver nanoparticle size, uniform in dispersion, low in material price, low in cost and good in antibacterial effect.
It should be noted that the above-mentioned embodiments illustrate rather than limit the invention, and that those skilled in the art will be able to design alternative embodiments without departing from the scope of the appended claims. In the claims, the word "comprising" does not exclude the presence of data or steps not listed in a claim.
Claims (9)
1. A preparation method of a polydopamine modified molybdenum disulfide composite silver nanoparticle antibacterial agent is characterized by comprising the following steps:
(1) molybdenum disulfide (MoS) with Polydopamine (PDA)2) Surface modification is carried out to prepare molybdenum disulfide @ polydopamine (MoS)2@ PDA) nano-sheet, preparing molybdenum disulfide @ polydopamine (MoS)2@ PDA) nanosheet solution;
(2) preparing a silver nitrate transparent solution, and measuring 25-28% ammonia water, wherein the molar ratio of the ammonia water to the silver nitrate is 50-100: 1, the molar ratio of silver nitrate to molybdenum disulfide is 1-50: 1;
(3) preparing a reducing agent glucose solution, wherein the molar ratio of glucose to silver nitrate is 10: 1;
(4) adding the solution obtained in the step (1) and the solution obtained in the step (2) into a wide-mouth bottle, magnetically stirring for 20-30 min for mixing, adding the glucose solution prepared in the step (3), and reducing by adopting one of a magnetic stirring mode for 1-1.5 h and a microwave reaction mode at 60 ℃ for 10 min;
(5) and (3) cleaning the product obtained in the step (4) by using ultrapure water, and then carrying out centrifugal purification, wherein the obtained final product is dispersed in the ultrapure water and stored in a refrigerator at 4 ℃.
2. The preparation method of the polydopamine-modified molybdenum disulfide composite silver nanoparticle antibacterial agent according to claim 1, wherein in the step (1), the preparation method of the molybdenum disulfide @ polydopamine nanosheet is as follows:
a. respectively preparing a dopamine transparent solution and a molybdenum disulfide solution, wherein the molar ratio of dopamine to molybdenum disulfide is 5: 1;
b. preparing 20 mM Tris-HCl buffer solution with the concentration of 10mM and the pH value of 8.5;
c. and (b) adding the dopamine solution and the molybdenum disulfide solution in the step a into the Tris-HCl buffer solution in the step b, magnetically stirring for 5min for uniform mixing, transferring the mixed solution into a 35mL special microwave tube, and then placing the special microwave tube on a microwave reactor for heating for 10min, wherein the microwave power is 100W, the pressure is 150PSI, and the heating temperature is 60 ℃.
3. The preparation method of the polydopamine-modified molybdenum disulfide composite silver nanoparticle antibacterial agent according to claim 1, wherein in the step (1), the preparation method of the molybdenum disulfide @ polydopamine nanosheet is as follows:
a. respectively preparing a dopamine transparent solution and a molybdenum disulfide solution, wherein the molar ratio of dopamine to molybdenum disulfide is 5: 1;
b. preparing 20 mM Tris-HCl buffer solution with the concentration of 10mM and the pH value of 8.5;
c. performing water bath ultrasonic reaction: and c, transferring the solutions prepared in the step a and the step b into a wide-mouth bottle for mixing, and then placing the wide-mouth bottle into a water bath ultrasonic instrument for water bath ultrasonic reaction, wherein the ultrasonic power is 53KHz, the ultrasonic time is 1-6 h, and the ultrasonic temperature is 20-40 ℃.
4. The preparation method of the polydopamine-modified molybdenum disulfide composite silver nanoparticle antibacterial agent according to claim 1, wherein in the step (5), the centrifugal rotation speed is 8000rpm-12000rpm, and the centrifugal time is 20 min-40 min.
5. A preparation method of a polydopamine modified molybdenum disulfide composite silver nanoparticle antibacterial agent is characterized by comprising the following steps:
(1) molybdenum disulfide (MoS) with Polydopamine (PDA)2) Surface modification is carried out to prepare molybdenum disulfide @ polydopamine (MoS)2@ PDA) nano-sheet, preparing 5mL, 150 mug/mL molybdenum disulfide @ polydopamine (MoS)2@ PDA) nanosheet solution;
(2) preparing 0.8mL and 10mg/mL silver nitrate solutions, and measuring 90 mu L of 25-28% ammonia water;
(3) preparing 9.3mL and 10mg/mL glucose solution;
(4) adding the solutions in the step (1) and the step (2) into a 50mL wide-mouth bottle, magnetically stirring for 30min for mixing, adding the glucose solution prepared in the step (3), and continuously magnetically stirring for reaction for 1.5h for reduction;
(5) and (3) washing the product obtained in the step (4) by adopting ultrapure water for multiple times, and then carrying out centrifugal purification, wherein the centrifugal rotation speed is 12000rpm, the centrifugal time is 40min, and the obtained final product is dispersed in the ultrapure water and stored in a refrigerator at the temperature of 4 ℃.
6. A preparation method of a polydopamine modified molybdenum disulfide composite silver nanoparticle antibacterial agent is characterized by comprising the following steps:
(1) molybdenum disulfide (MoS) with Polydopamine (PDA)2) Surface modification is carried out to prepare molybdenum disulfide @ polydopamine (MoS)2@ PDA) nano-sheet, preparing 5mL, 150 mug/mL molybdenum disulfide @ polydopamine (MoS)2@ PDA) nanosheet solution;
(2) preparing 0.8mL and 10mg/mL silver nitrate solutions, and measuring 180 mu L of 25-28% ammonia water;
(3) preparing 9.3mL and 10mg/mL glucose solution;
(4) adding the solutions in the step (1) and the step (2) into a wide-mouth bottle, magnetically stirring for 20min, mixing, adding the glucose solution prepared in the step (3), and reacting at 60 ℃ for 10min by microwave for reduction;
(5) and (3) washing the product obtained in the step (4) by adopting ultrapure water for multiple times, and then carrying out centrifugal purification, wherein the centrifugal rotation speed is 12000rpm, the centrifugal time is 20min, and the obtained final product is dispersed in the ultrapure water and stored in a refrigerator at the temperature of 4 ℃.
7. A preparation method of a polydopamine modified molybdenum disulfide composite silver nanoparticle antibacterial agent is characterized by comprising the following steps:
(1) molybdenum disulfide (MoS) with Polydopamine (PDA)2) Surface modification is carried out to prepare molybdenum disulfide @ polydopamine (MoS)2@ PDA) nano-sheet, preparing 5mL, 150 mug/mL molybdenum disulfide @ polydopamine (MoS)2@ PDA) nanosheet solution;
(2) preparing 80 mu L of silver nitrate solution with the concentration of 10mg/mL, and measuring 18 mu L of ammonia water with the concentration of 25-28%;
(3) preparing 0.93mL and 10mg/mL glucose solution;
(4) adding the solutions in the step (1) and the step (2) into a wide-mouth bottle, magnetically stirring for 20min for mixing, adding the glucose solution prepared in the step (3), and continuing to magnetically stir for reaction for 1h for reduction;
(5) and (3) washing the product obtained in the step (4) for multiple times by using ultrapure water, and then carrying out centrifugal purification, wherein the centrifugal rotation speed is 8000rpm, the centrifugal time is 30min, and the obtained final product is dispersed in the ultrapure water and stored in a refrigerator at the temperature of 4 ℃.
8. A preparation method of a polydopamine modified molybdenum disulfide composite silver nanoparticle antibacterial agent is characterized by comprising the following steps:
(1) with polydopamine (P)DA) vs. molybdenum disulfide (MoS)2) Surface modification is carried out to prepare molybdenum disulfide @ polydopamine (MoS)2@ PDA) nano-sheet, preparing 5mL, 150 mug/mL molybdenum disulfide @ polydopamine (MoS)2@ PDA) nanosheet solution;
(2) preparing 4mL of silver nitrate solution and 10mg/mL of silver nitrate solution, and measuring 900 mu L of 25-28% ammonia water;
(3) preparing 4.65mL of glucose solution and 100mg/mL of glucose solution;
(4) adding the solutions in the step (1) and the step (2) into a wide-mouth bottle, magnetically stirring for 30min for mixing, adding the glucose solution prepared in the step (3), and continuing to magnetically stir for reaction for 1.5h for reduction;
(5) and (3) washing the product obtained in the step (4) by adopting ultrapure water for multiple times, and then carrying out centrifugal purification, wherein the centrifugal rotation speed is 12000rpm, the centrifugal time is 40min, and the obtained final product is dispersed in the ultrapure water and stored in a refrigerator at the temperature of 4 ℃.
9. A polydopamine-modified molybdenum disulfide composite silver nanoparticle antibacterial agent, characterized in that the polydopamine-modified molybdenum disulfide composite silver nanoparticle antibacterial agent is prepared according to any one of claims 1 to 8.
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