CN108477220B - Aspergillus parasiticus spore composite pesticide and preparation method thereof - Google Patents
Aspergillus parasiticus spore composite pesticide and preparation method thereof Download PDFInfo
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Abstract
The invention provides an aspergillus parasiticus spore composite pesticide which comprises 80-90% of active components and 10-20% of auxiliary agents in percentage by mass, wherein the active components comprise rotenone powder and aspergillus parasiticus spore powder, the mass ratio of the rotenone powder to the aspergillus parasiticus spore powder is 1 (15-20), and the spore number of the aspergillus parasiticus spore powder is more than 8 multiplied by 108Per gram. The aspergillus parasiticus spore compound pesticide adopts the combination of rotenone and aspergillus parasiticus to form a pest killing mechanism of 'paralysis firstly, parasitism secondly, killing secondly, epidemic later', and effectively controls the propagation of pest population. Meanwhile, the invention also provides a preparation method of the aspergillus parasiticus spore composite pesticide.
Description
Technical Field
The invention belongs to the field of biological pesticides, and particularly relates to an aspergillus parasiticus spore composite pesticide and a preparation method thereof.
Background
China is a big agricultural country, pests such as insects, acarines (mites and ticks), aphids and nematodes are common agricultural pests, and economic losses caused by the pests are huge every year. In order to control insect pests, various insecticides have been developed. The existing insecticides mainly comprise chemical insecticides, botanical insecticides and fungal insecticides.
Among them, chemical insecticides are used to control pests, and the killing effect is very significant in a short time, but excessive use of chemical insecticides leaves residues in soil, water and air, and also has adverse effects on non-target organisms and ecological balance. In addition, pests are susceptible to developing resistance to chemical pesticides, limiting their efficacy and application. Concern over the potential health hazards of chemical pesticides and the increased cost of chemical pesticides have also prompted the development of environmentally friendly pest management strategies.
The botanical pesticide has the advantages of safety, high efficiency, low toxicity, low residue and the like which can not be compared favorably with chemical pesticides, has a very positive effect on solving the problems brought by the chemical pesticides, is a hotspot of the current innovative pesticide development, but is still faced with a plurality of problems when being widely applied to agricultural production as a novel pesticide product by replacing the chemical pesticides, particularly has strong pertinence to the pesticide killing of the botanical pesticide and does not have broad spectrum, which is the advantage of the botanical pesticide, but also limits the application of the botanical pesticide in production practice, can kill a certain specific pest and disease and insect damage, protects the natural enemy of the pest, simultaneously limits the application range of the botanical pesticide to be only applied to a certain or a plurality of specific crops, and has the defects of short control effect and no persistence; the fungal pesticide has the advantages of wide host range, safety to environment, no drug resistance in pest control and the like, so that the fungal pesticide is widely concerned in research and application, but many fungi are used for controlling one pest and have no broad spectrum.
At present, with the increasing demand of people on insecticides, single-action insecticides can not meet the demand of people gradually, and the development of multifunctional compound insecticides is an important development trend.
Disclosure of Invention
In view of the above, the present invention provides an aspergillus parasiticus spore composite pesticide and a preparation method thereof, so as to solve the above problems.
Specifically, the invention adopts the following technical scheme:
the aspergillus parasiticus spore composite pesticide comprises 80-90% of active components and 10-20% of auxiliary agents by mass percent, wherein the active components comprise rotenone powder and aspergillus parasiticus spore powder, the mass ratio of the rotenone powder to the aspergillus parasiticus spore powder is 1 (15-20), and the spore number of the aspergillus parasiticus spore powder is more than 8 multiplied by 108Per gram.
The insecticidal mechanism of rotenone is as follows: the respiration of insects is affected, the electron transfer chain of insect cells is inhibited, the ATP level in organisms is reduced, the insects cannot be supplied with energy, and then the insects die slowly due to sluggish action and paralysis.
The insecticidal mechanism of aspergillus parasiticus is as follows: the process from spore germination to infection to thallus again produces spore on the polypide is divided into 10 stages, namely, spore is attached to the epidermis of the host, spore germination, hypha penetrates the epidermis, hypha grows in the blood cavity, toxin is produced, the host dies, hypha invades all organs of the host, hypha penetrates out of the epidermis, spore is produced, and the spreading (namely, the prevalence) of infection units.
The preparation method of the aspergillus parasiticus spore powder comprises the following steps: inoculating the parasitic aspergillus into a PDA liquid culture medium, placing the PDA liquid culture medium on a shaking bed, culturing for 24 hours at the temperature of 28 ℃ to prepare hypha liquid seeds, and inoculating the hypha liquid seeds into a culture medium prepared by mixing the following components in percentage by mass of 5 percent: 1: 1, maintaining air humidity at 50%, culturing at 28 deg.C for 5 days, air drying the solid culture medium after spore is covered on the surface of the solid culture medium, placing in a pulverizing sieve after air drying, separating Aspergillus parasiticus spore, counting viable bacteria in PDA solid culture medium, determining spore powder content, diluting with diatomite to obtain product with content of more than 8 × 108Each gram of spore powder is ready for use.
Aspergillus parasiticus RWSF001-02 is prepared by placing infected aphid into solid plate of PDA culture medium, culturing at 28 deg.C for 48 hr, picking single colony, separating, performing indoor aphid preventing and treating effect test on several separated strains, and repeatedly screening to obtain Aspergillus parasiticus with high insecticidal activity, and is applied for patent (CN 201610919233.0).
Based on the above, the preparation method of the rotenone powder comprises the following steps: pulverizing the roots of rotenone, and sieving with a 120-mesh sieve to obtain the rotenone powder.
Based on the above, the auxiliary agent comprises, by mass, 2-4 parts of a spore germination promoter, 5-6 parts of a dispersing agent, 2-3 parts of an ultraviolet-resistant protective agent, 1-2 parts of a stabilizer and 2-3 parts of a wetting agent.
Based on the above, the spore germination accelerant comprises, by mass, 0.1% -0.2% of peptone, 0.03% -0.07% of yeast powder, 0.05% -0.15% of glucose, 0.05% -0.15% of ammonium sulfate, 0.05% -0.15% of dipotassium hydrogen phosphate and 92% -97% of diatomite.
Based on the above, the aspergillus parasiticus spore compound pesticide is in the dosage forms of wettable powder, suspending agent, dispersible oil suspending agent, microcapsule suspending agent, missible oil or microemulsion.
Based on the above, the wettable powder comprises, by mass, 80-90% of active ingredients, 2-4% of a spore germination promoter, 5-6% of a calcium lignosulfonate dispersant, 2-3% of a sodium fluorescein anti-ultraviolet protective agent, 1-2% of an oxalic acid stabilizer and 2-3% of a polyethylene glycol wetting agent.
The preparation method of the aspergillus parasiticus spore composite pesticide comprises the step of uniformly mixing the active component and the auxiliary agent.
The Aspergillus parasiticus spore composite pesticide comprises 80-90% of active components and 10-20% of auxiliary agents by mass percent, wherein the active components comprise rotenone and Aspergillus parasiticus, the mass ratio of the rotenone to the Aspergillus parasiticus is 1 (15-20), the Aspergillus parasiticus is Aspergillus parasiticus RWSF001-02, is preserved in Guangdong province microbial strain preservation center, and has the preservation number of GDMCC NO: 60080.
compared with the prior art, the invention has outstanding substantive characteristics and remarkable progress. In particular, the aspergillus parasiticus spore composite pesticide provided by the invention takes rotenone and aspergillus parasiticus spore powder as active components, in the using process, the rotenone firstly paralyzes pests, the paralyzed pests are more favorable for parasitizing the aspergillus parasiticus spore powder under the action of the spore germination accelerant, thereby killing and diffusing pests, simultaneously overcoming the defects of short and non-persistent control effect of the single dose of the rotenone powder on the pests, therefore, the aspergillus parasiticus spore composite pesticide provided by the invention adopts the combination of rotenone and aspergillus parasiticus to form a pesticide action mechanism of paralysis firstly, parasitism secondly, killing secondly and epidemic secondly, has obvious synergistic action, not only can ensure that the composite pesticide has broad spectrum, the insecticidal composition can be widely used for killing pests such as mites, red spiders, scale insects, aphids and the like, effectively controls the propagation of pest populations, and improves the insecticidal effect and the insecticidal efficiency; meanwhile, the rotenone powder is a botanical pesticide, the aspergillus parasiticus is a fungal pesticide, and the combination of the rotenone powder and the aspergillus parasiticus can effectively avoid the adverse effect on the ecological environment caused by using a chemical pesticide, so that the aspergillus parasiticus spore compound pesticide provided by the invention is a green pesticide. The invention also provides a preparation method of the aspergillus parasiticus spore composite pesticide, which is simple, easy to operate and suitable for industrial application.
Detailed Description
The technical solution of the present invention is further described in detail by the following embodiments.
Example 1
This example provides an Aspergillus parasiticus spore compound pesticide, which is a wettable powder and comprises, by mass, 5.5% of calcium lignosulfonate, 2.5% of sodium fluorescein, 1.5% of oxalic acid, 2.5% of polyethylene glycol, 3% of a spore germination promoter, 5% of rotenone powder and 80% of spore powder (Aspergillus parasiticus rwsf 001-02); wherein the spore germination accelerant comprises 0.15% of peptone, 0.05% of yeast powder, 0.1% of glucose, 0.1% of ammonium sulfate, 0.1% of dipotassium hydrogen phosphate and 95% of diatomite.
The preparation method of the aspergillus parasiticus spore composite pesticide comprises the following steps:
1) preparation of aspergillus parasiticus spore powder: inoculating the parasitic aspergillus into a PDA liquid culture medium, placing the PDA liquid culture medium on a shaking bed, culturing for 24 hours at the temperature of 28 ℃ to prepare hypha liquid seeds, and inoculating the hypha liquid seeds into a culture medium prepared by mixing the following components in percentage by mass of 5 percent: 1: 1, maintaining air humidity at 50%, culturing at 28 deg.C for 5 days, air drying the solid culture medium after spore is covered on the surface of the solid culture medium, placing in a pulverizing sieve after air drying, separating Aspergillus parasiticus spore, counting viable bacteria in PDA solid culture medium, determining spore powder content, diluting with diatomite to obtain product with content of more than 8 × 108Spore powder per gramAnd (5) standby.
2) Preparing a spore germination promoter:
the spore germination accelerant is prepared by compounding peptone of 0.15 percent, yeast powder of 0.05 percent, glucose of 0.1 percent, ammonium sulfate of 0.1 percent, dipotassium hydrogen phosphate of 0.1 percent and diatomite of 95 percent according to mass fraction for later use.
3) Preparing rotenone powder:
pulverizing the roots of rotenone, and sieving with a 120-mesh sieve to obtain rotenone powder for later use;
4) preparing wettable powder:
and uniformly mixing calcium lignosulphonate, fluorescein sodium, oxalic acid, polyethylene glycol, a spore germination promoter, rotenone powder and spore powder to obtain the aspergillus parasiticus spore wettable powder.
Example 2
This example provides an aspergillus parasiticus spore composite pesticide, and differs from example 1 in that: according to the mass percentage, the fertilizer comprises 5 percent of calcium lignosulphonate, 2 percent of sodium fluorescein, 1 percent of oxalic acid, 2 percent of polyethylene glycol, 2 percent of spore germination accelerant, 5 percent of rotenone powder and 83 percent of spore powder (Aspergillus parasitilis RWSF 001-02); wherein the spore germination accelerant comprises 0.1% of peptone, 0.03% of yeast powder, 0.05% of glucose, 0.05% of ammonium sulfate, 0.15% of dipotassium hydrogen phosphate and 96.4% of diatomite.
Example 3
This example provides an aspergillus parasiticus spore composite pesticide, and differs from example 1 in that: the fertilizer comprises, by mass, 6% of calcium lignosulphonate, 3% of sodium fluorescein, 2% of oxalic acid, 3% of polyethylene glycol, 4% of a spore germination promoter, 5% of rotenone powder and 77% of spore powder (Aspergillus parasitilis RWSF 001-02); wherein the spore germination accelerant is 0.2% of peptone, 0.07% of yeast powder, 0.15% of glucose, 0.15% of ammonium sulfate, 0.15% of dipotassium hydrogen phosphate and 97% of diatomite.
Performance verification
The following comparative insecticides were prepared first: a insecticide is rotenone powder single agent; a class B insecticide which differs from the composite insecticide described in example 1 in that the insecticide does not contain rotenone powder. To verify the effectiveness of the Aspergillus parasiticus spore composite pesticide of the present invention, a comparative test was conducted using the class A pesticide, the class B pesticide and the wettable powder of example 1.
Test one: the test field is arranged in a vegetable greenhouse in a Deyun vineyard in Zhengzhou city, and the variety of the crop to be tested is cherry tomato red spider. The test treatments were as follows: processing one: dissolving 1.5g of pesticide A in 20kg of clear water, and mixing uniformly for later use; and (5) processing: dissolving 30g of pesticide B in 20kg of clear water, and uniformly mixing for later use; and (3) treatment III: 30g of the wettable powder is dissolved in 20kg of clear water, mixed evenly for standby and subjected to clear water blank control. Area of 15m per test treatment cell2Repeating the test for 3 times, randomly arranging the cells in blocks, spraying with a knapsack manual sprayer, and spraying at a rate of 20kg/667m2Spraying before evening to ensure that the relative humidity in the shed is more than or equal to 85 percent. To prevent process interference, the adjacent cells are covered with a plastic film during application. Sampling at 5 points in a cell before treatment, selecting 2 plants at each point, calibrating 3 leaves with the parasitic quantity of not less than 50 red spiders on the selected plants, investigating the population base number, counting the death conditions of the red spiders 3d, 5d and 7d after treatment, and calculating the prevention and treatment effect, wherein the result is shown in table 1, and the specific calculation formula is as follows:
percent reduction of population (%) (population before treatment-number of residual insects per day after treatment)/population before treatment X100%
Control effect (%) - (reduction rate of population in treatment area-reduction rate of population in control area)/(reduction rate of population in 100-control area) × 100%
TABLE 1 prevention and control effect of different effective components on red spider of cherry tomato
As can be seen from table 1, after the spraying treatment for 3d, the difference between the first treatment and the second treatment and the third treatment is significant, after the spraying treatment for 5d and 7d, the difference between the second treatment and the third treatment is not significant, but the combination of the third treatment is obviously superior to the combination of the first treatment and the second treatment, the prevention effect is more than 85% after the spraying day 5d compared with the first treatment, the re-propagation of the red spiders is controlled, the control time is shortened by two days compared with the second treatment, the control time of the red spiders is effectively shortened by combining the rotenone and the aspergillus parasiticus, and the control efficiency is improved. Effectively overcomes the defects of short control effect and non-persistence of the botanical insecticide rotenone powder. Therefore, the aspergillus parasiticus spore compound pesticide also has good control effect on red spiders.
And (2) test II: the test field is arranged in a vegetable greenhouse of Biotechnology Limited company of Biaorui, Beijing, and the test article species is green pepper tea yellow mite which is treated as follows: processing one: dissolving 1.5g of pesticide A in 20kg of clear water, and mixing uniformly for later use; and (5) processing: dissolving 30g of pesticide B in 20kg of clear water, and uniformly mixing for later use; and (3) treatment III: and dissolving 30g of the wettable powder in 20kg of clear water, uniformly mixing for later use, and performing clear water blank control. Each processing cell area 15m2Repeat 3 times, and arrange the random block of the cell. Spraying with a knapsack manual sprayer, wherein the spraying liquid amount is 20kg/667m2Spraying before evening to ensure that the relative humidity in the shed is more than or equal to 85 percent, and in order to prevent mutual interference between treatments, a plastic film is used for covering and separating adjacent cells during application. Sampling is carried out at 3 points in a cell before treatment, 2 plants are selected at each point, 3 leaves with the parasitic amount of not less than 30 of tea yellow mites are calibrated on the selected plants, the population base number is investigated, the death condition of the tea yellow mites is counted at 3d, 5d and 7d after spraying treatment, the prevention and treatment effect is calculated, and the result is shown in table 2.
TABLE 2 control effect of different effective components on green pepper tea yellow mite
As can be seen from table 2, after the spraying treatment for 3d, the difference between the first treatment and the second treatment is significant, the difference between the second treatment and the third treatment is not significant, and after the spraying treatment for 5d and 7d, the difference between the second treatment and the third treatment is not significant, but the combination of the third treatment is obviously superior to the first treatment and the second treatment, compared with the first treatment, the control effect reaches more than 80% after the spraying treatment for 5d, the re-propagation of the tea yellow mites is basically controlled, compared with the second treatment, the control time is shortened by two days, and by combining the rotenone with the aspergillus parasiticus, the control time of the tea yellow mites is effectively shortened, and the control efficiency is improved. Therefore, the aspergillus parasiticus spore compound pesticide also has a good prevention and control effect on yellow tea mites.
And (3) test III: the test field is arranged in a greenhouse of Biaorui biological technology Co., Ltd, Beijing, the variety of the crop to be tested is peach scale insects, and the test treatment is as follows: processing one: dissolving 1.5g of pesticide A in 20kg of clear water, and mixing uniformly for later use; and (5) processing: dissolving 30g of pesticide B in 20kg of clear water, and uniformly mixing for later use; and (3) treatment III: 30g of the wettable powder is dissolved in 20kg of clear water, mixed evenly for standby and subjected to clear water blank control. Each processing cell area 15m2Repeat 3 times, and arrange the random block of the cell. Spraying with a knapsack manual sprayer, wherein the spraying liquid amount is 20kg/667m2Spraying before evening to ensure that the relative humidity in the shed is more than or equal to 85 percent, and in order to prevent mutual interference between treatments, a plastic film is used for covering and separating adjacent cells during application. Sampling is carried out at 3 points in a cell before treatment, 2 plants are selected at each point, 3 areas of investigation with the number of the coccid parasitic quantity not less than 30 are marked on the selected plants, the population base number is investigated, the death condition of the coccid is counted at 3d, 5d and 7d after spraying treatment, the prevention and treatment effect is calculated, and the result is shown in table 3.
TABLE 3 prevention and treatment effects of different effective components on coccid of peach tree
As can be seen from table 3, after the spraying treatment for 3d, the difference between the first treatment and the second treatment is significant, the difference between the second treatment and the third treatment is not significant, and after the spraying treatment for 5d and 7d, the difference between the second treatment and the third treatment is not significant, but the combination of the third treatment is obviously superior to the combination of the first treatment and the second treatment, compared with the first treatment, the control effect reaches more than 50% after the seventh day of spraying, the damage of the coccid to the peach trees is reduced, the control time of the coccid is shortened by combining the rotenone and the aspergillus parasiticus, and the control efficiency is improved. Therefore, the aspergillus parasiticus spore compound pesticide also has a certain control effect on the scale insects.
And (4) testing: the test field is arranged in a vegetable greenhouse in a Deyu county and a vineyard in Zhengzhou city, and the crop variety to be tested is the zucchini aphids. The test treatments were as follows: processing one: dissolving 1.5g of pesticide A in 20kg of clear water, and mixing uniformly for later use; and (5) processing: dissolving 30g of pesticide B in 20kg of clear water, and uniformly mixing for later use; and (3) treatment III: 30g of the wettable powder is dissolved in 20kg of clear water, mixed evenly for standby and subjected to clear water blank control. Area of 15m per test treatment cell2Repeating the test for 3 times, randomly arranging the cells in blocks, spraying with a knapsack manual sprayer, and spraying at a rate of 20kg/667m2Spraying before evening to ensure that the relative humidity in the shed is more than or equal to 85 percent. To prevent process interference, the adjacent cells are masked with a plastic film when the drug is applied. Sampling is carried out at 5 points in a cell before treatment, 2 plants are selected at each point, 3 leaves with the parasitic quantity of no-wing adult aphids not less than 50 are calibrated on the selected plants, the population base number is investigated, the death condition of the aphids is counted at 3d, 5d and 7d after treatment, the prevention and treatment effect is calculated, the result is shown in table 4, and the specific calculation formula is as follows:
percent reduction of population (%) (population before treatment-number of residual insects per day after treatment)/population before treatment X100%
Control effect (%) - (reduction rate of population in treatment area-reduction rate of population in control area)/(reduction rate of population in 100-control area) × 100%
TABLE 4 control effect of different effective components on zucchini aphid
As can be seen from table 4, after the spraying treatment for 3d, the differences between the first treatment and the second treatment and the third treatment are significant, after the spraying treatment for 5d and 7d, the differences between the second treatment and the third treatment are not significant, but the combination of the third treatment is obviously superior to the combination of the first treatment and the second treatment, the control effect reaches more than 96% after the third day of spraying compared with the first treatment, the re-breeding of aphids is controlled, the control time is shortened by two days compared with the second treatment, and the control time of the aphids is effectively shortened and the control efficiency is improved by combining the rotenone and the aspergillus parasiticus. Effectively overcomes the defects of short control effect and non-persistence of the botanical insecticide rotenone powder. Therefore, the aspergillus parasiticus spore compound pesticide has the advantages of strong specificity, no pollution and continuous control on aphids.
With the comprehensive table 1-4, the aspergillus parasiticus spore composite pesticide provided by the invention can effectively kill aphids, mites, scale insects and red spiders, has broad spectrum, effectively overcomes the defects of short control effect and non-persistence of a single plant source pesticide, and has good application prospect.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention and not to limit it; although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art will understand that: modifications to the specific embodiments of the invention or equivalent substitutions for parts of the technical features may be made; without departing from the spirit of the present invention, it is intended to cover all aspects of the invention as defined by the appended claims.
Claims (4)
1. The aspergillus parasiticus spore compound pesticide is wettable powder and is characterized by comprising 80-90% of active components and 10-20% of auxiliary agents in percentage by mass, wherein the active components comprise rotenone powder and aspergillus parasiticus spore powder, the mass ratio of the rotenone powder to the aspergillus parasiticus spore powder is 1 (15-20), and the spore number of the aspergillus parasiticus spore powder is more than 8 multiplied by 108Per gram;
the Aspergillus parasiticus spore powder is obtained by culturing Aspergillus parasiticus RWSF001-02, the Aspergillus parasiticus RWSF001-02 is preserved in Guangdong province microorganism culture collection, and the preservation number is GDMCC NO: 60080, respectively;
the auxiliary agent comprises, by mass, 2-4 parts of a spore germination promoter, 5-6 parts of a dispersing agent, 2-3 parts of an ultraviolet-resistant protective agent, 1-2 parts of a stabilizing agent and 2-3 parts of a wetting agent.
2. The Aspergillus parasiticus spore composite pesticide according to claim 1, characterized in that the spore germination promoter comprises 0.1-0.2% of peptone, 0.03-0.07% of yeast powder, 0.05-0.15% of glucose, 0.05-0.15% of ammonium sulfate, 0.05-0.15% of dipotassium hydrogen phosphate and 92-97% of diatomite by mass percentage.
3. An Aspergillus parasiticus spore composite pesticide according to claim 2, characterized in that the wettable powder comprises 80-90% of active component, 2-4% of spore germination accelerating agent, 5-6% of calcium lignosulfonate dispersant, 2-3% of sodium fluorescein anti-ultraviolet protective agent, 1-2% of oxalic acid stabilizer and 2-3% of polyethylene glycol wetting agent in percentage by mass.
4. A method of preparing an Aspergillus parasiticus spore composite pesticide according to any one of claims 1 to 3, characterized by comprising the step of uniformly mixing the active component and the auxiliary agent.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103734168A (en) * | 2014-01-10 | 2014-04-23 | 南京盈植康农业科技有限公司 | Insecticidal pesticide formulation containing biopesticide-rotenone |
| CN104222161A (en) * | 2014-08-29 | 2014-12-24 | 广东省农业科学院植物保护研究所 | Insecticide composition as well as preparation method and application of insecticide composition |
| CN106376582A (en) * | 2016-08-30 | 2017-02-08 | 广西世超生物科技有限公司 | Rotenone water dispersible granule and preparation method thereof |
| CN106520564A (en) * | 2016-10-21 | 2017-03-22 | 鹤壁市人元生物技术发展有限公司 | Aspergillus parasiticus and preparation method and application thereof |
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- 2018-02-01 CN CN201810102989.5A patent/CN108477220B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103734168A (en) * | 2014-01-10 | 2014-04-23 | 南京盈植康农业科技有限公司 | Insecticidal pesticide formulation containing biopesticide-rotenone |
| CN104222161A (en) * | 2014-08-29 | 2014-12-24 | 广东省农业科学院植物保护研究所 | Insecticide composition as well as preparation method and application of insecticide composition |
| CN106376582A (en) * | 2016-08-30 | 2017-02-08 | 广西世超生物科技有限公司 | Rotenone water dispersible granule and preparation method thereof |
| CN106520564A (en) * | 2016-10-21 | 2017-03-22 | 鹤壁市人元生物技术发展有限公司 | Aspergillus parasiticus and preparation method and application thereof |
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