CN108467833A - A kind of magnetotactic bacteria cultural method - Google Patents
A kind of magnetotactic bacteria cultural method Download PDFInfo
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- CN108467833A CN108467833A CN201810283228.4A CN201810283228A CN108467833A CN 108467833 A CN108467833 A CN 108467833A CN 201810283228 A CN201810283228 A CN 201810283228A CN 108467833 A CN108467833 A CN 108467833A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
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Abstract
The invention belongs to Bacteria Culture technical fields, specifically a kind of magnetotactic bacteria cultural method, the process employs magnetotactic bacteria culture devices, wherein culture device includes babinet, case lid, shakes module, clamp module and liquid feeding module, babinet is rectangle uncovered babinet, and the left and right sidewall of babinet symmetrically opens up left groove and right groove；Case lid is horizontally arranged at top of the box；It shakes module and the inner bottom in babinet is set, shake module for making magnetotactic bacteria rock to promote incubation；Clamp module is arranged in the top for shaking module, and the device that clamp module is used to hold magnetotactic bacteria clamps；Liquid feeding module is arranged in the top of clamp module, liquid feeding module is used to that culture solution to be added to box house, present invention is mainly used for culture magnetotactic bacterias, mutual cooperation realization by shaking module and clamp module carries out culture solution to shake up mixing, it is set fully to react, the replacement of culture solution may be implemented in liquid feeding module, substantially increases the culture efficiency of magnetotactic bacteria.
Description
Technical field
The invention belongs to Bacteria Culture technical field, specifically a kind of magnetotactic bacteria cultural method.
Background technology
Magnetotactic bacteria, which is one kind, can make directed movement under the action of external magnetic field and form nano magnetic particle-in vivo
The bacterium of magnetic corpusculum is distributed mainly in soil, lake and ocean.
Bacteria Culture is a kind of technology for by artificial means breeding bacterial growth.Bacterium distributed pole in nature is wide,
Quantity is big, and type is more, it can promote the well-being of mankind, and can also become pathogenic reason.Most of bacteriums can be trained by artificial means
It supports, that is, is inoculated on culture medium, growth is made to breed.The bacterium come is turned out for studying, identifying and applying.Bacterium
Culture is a complicated technology.
Current existing classical culture protocols are mostly that inoculum is put into culture medium, it is allowed under conditions of closed
Natural propagation, but such method efficiency is relatively low, and long the time required to magnetotactic bacteria breeding, be unfavorable for production process into
Row.
Invention content
In order to make up for the deficiencies of the prior art, the present invention proposes a kind of magnetotactic bacteria cultural method, and the present invention mainly uses
In a kind of cultural method of magnetotactic bacteria of offer.The present invention makes culture apparatus put by the clamp module being arranged in culture device
Horizontalization is steady, then the effect by shaking module makes magnetotactic bacteria bacterium solution and culture solution be sufficiently mixed, and greatly improves the breeding of bacterium
Efficiency.
The technical solution adopted by the present invention to solve the technical problems is：A kind of magnetotactic bacteria culture side of the present invention
Method, this method use following culture device, the culture device to include babinet, case lid, shake module, clamp module and liquid feeding mould
Block, the babinet are rectangle uncovered babinet, and the left and right sidewall of babinet symmetrically opens up left groove and right groove；The case lid water
Safety is mounted in top of the box；The inner bottom in babinet is arranged in the shaking module, shakes module for making bacterium rock
To promote incubation；The clamp module is arranged in the top for shaking module, and clamp module will be for that will hold magnetotactic bacteria
Device clamps, and improves device stability when shaking；The liquid feeding module is arranged in the top of clamp module, and liquid feeding module is used for
Inoculum is added to cultivate magnetotactic bacteria to box house；
The cultural method includes the following steps：
Step 1：Open equipment：To babinet is placed two in 30 DEG C of ± 1 DEG C of air-conditioned rooms after babinet progress sterilization treatment
Hour allows it to keep constant temperature；
Step 2：With liquid：The brine containing 3% salt is taken, and yeast powder, peptone and glycerine is added, adjusts PH to 7.0,130
DEG C sterilizing 20 minutes, K is added after sterilizing2HPO4Step is added in solution after being cooled to room temperature the magnetotactic bacteria culture solution configured
In culture dish in a rapid middle case；
Step 3：It first passes through control clamp module to clamp culture dish, then takes magnetotactic bacteria bacterium solution from upper layer liquid-feeding tube
Center is added in the culture dish of step 2, controls magnetotactic bacteria culture solution and magnetotactic bacteria bacterium solution mixes 30 minutes；
Step 4：Open take-up motor, control shake module to the magnetotactic bacteria culture solution in culture dish in step 3 into
Row rocks mixing, and magnetotactic bacteria culture solution and magnetotactic bacteria are sufficiently mixed；
Step 5：After culture solution in step 4 has been rocked a period of time, liquid feeding motor, control lower layer liquid-feeding tube are opened
The magnetotactic bacteria bacterium solution in culture dish is carried out changing liquid into culture dish, to promote the breeding of magnetotactic bacteria in culture dish
Journey.
The shaking module includes take-up motor, take-up spring, roller, steel wire rope, support spring and holds ware, the case
Take-up motor is installed in the left groove of body, take-up spring is horizontally mounted in right groove；The roller is placed in babinet bottom
The heart, opens up No.1 groove on roller, and lay winding wire ropes in No.1 groove, one end of steel wire rope is connect with take-up motor, steel wire rope
The other end connect with take-up spring；The support spring is connected to the bottom of box vertically, and support spring is arranged two；It is described
It holds ware to be placed on roller, it is arc to hold ware outer bottom, and inside is planar structure, holds ware both ends and support spring
Top connection, the inner horizontal for holding ware opens up No.1 sliding slot.When work, take-up motor is opened, take-up motor pulls steel wire
Rope, steel wire rope drive roller to move left and right, and roller, which moves left and right drive and holds ware, to be moved left and right.
The clamp module includes idler wheel, holder, electromagnet, No.1 spring and baffle, is installed in the No.1 sliding slot
Idler wheel, vertical connecting bracket on idler wheel, holder are L-type, and holder is arranged two；The internal stent level opens up No. two grooves,
Electromagnet is set in No. two grooves, and the other end of electromagnet horizontal connection No.1 spring, No.1 spring connects baffle.Work
When, electromagnet is by driving No.1 spring that baffle is driven to keep out holder on the side wall of No.1 sliding slot.
The liquid feeding module includes culture dish, magnetic patch, upper layer liquid-feeding tube, gear, liquid feeding motor, lower layer's liquid-feeding tube and tooth
Item, the holder centre connect culture dish, and culture dish is rectangle uncovered babinet；The magnetic patch is mounted on the side wall of culture dish
In；The upper layer liquid-feeding tube is vertically installed on case lid, and upper layer liquid-feeding tube is cylindrical shape, and the bottom of upper layer liquid-feeding tube passes through case
Lid is located at box house, and the lower half portion surface of upper layer liquid-feeding tube opens up sliding slot；The gear and liquid feeding motor are arranged on upper layer
Inside the entity of liquid-feeding tube；Lower layer's liquid-feeding tube, which is arranged inside the bottom of upper layer liquid-feeding tube, lower layer's liquid-feeding tube, installs tooth
Item, rack and pinion are meshed, and channel is opened up inside upper layer liquid-feeding tube and lower layer's liquid-feeding tube.When work, liquid feeding motor is opened,
Liquid feeding motor is rotated by sliding tooth wheel, gear by band carry-over bar to drive lower layer's liquid-feeding tube along sliding slot upward or downward
Movement realizes the merging and separation of upper layer liquid-feeding tube and lower layer's liquid-feeding tube, when there is liquid to be flowed into from the center of upper layer liquid-feeding tube
It can be flowed into culture dish along the center of lower layer's liquid-feeding tube, when needing to change liquid, by upper layer liquid-feeding tube and lower layer's liquid-feeding tube
Center is blocked, and liquid is made to be flowed out from channel.
The support level connects No. two springs, and the other end of No. two springs connects fixture block, and fixture block connects with culture dish side wall
It connects.It is more steady that the setting of No. two springs and fixture block makes culture dish be positioned to, and improves stability when equipment work.
The culture dish bottom opens up semicircular arc groove about central symmetry, and partition board is arranged in culture dish, and partition board is hinged
On the side wall of semicircular arc groove, vertical connection No.1 spring, the No.1 spring other end and semicircular arc groove below partition board
Bottom connects；Electromagnet is set inside the culture dish entity.When work, when needing to change liquid inside culture dish, pass through control
Switch opens electromagnet, and electromagnet attracts partition board to move downward, and magnetotactic bacteria enters in semicircular arc groove, when preventing from changing liquid
Magnetotactic bacteria is gone out from upper layer liquid-feeding tube and lower layer's liquid-feeding tube, is changed after the completion of liquid, and control switch is disconnected, and electromagnet loses magnetism,
Magnetotactic bacteria returns in culture dish, and partition board sets back in the effect of No.1 spring.
The beneficial effects of the invention are as follows：
1. a kind of magnetotactic bacteria cultural method of the present invention, the present invention includes Step 1: Step 2: step 3 and step
Rapid four, step 1 and step 2 belong to the preparation before magnetotactic bacteria culture, and step 3 is for mixing magnetotactic bacteria culture solution
Even, step 4 renews for magnetotactic bacteria culture solution, and the present invention cultivates magnetotactic bacteria by four steps, technique
Simply, cost it is relatively low, it can be achieved that magnetotactic bacteria it is effective culture and it is easily operated.
2. a kind of magnetotactic bacteria cultural method of the present invention, the bacteria breeding facility used in of the invention passes through setting
Sliding slot outside liquid feeding mould upper layer liquid-feeding tube in the block, make lower layer's liquid-feeding tube that can be slided up and down along upper layer liquid-feeding tube, it can be achieved that
Magnetotactic bacteria in culture dish changes liquid, improves the proliferative speed of magnetotactic bacteria.
3. a kind of magnetotactic bacteria cultural method of the present invention, the bacteria breeding facility used in of the invention passes through setting
The semicircular arc groove of culture dish bottom, when carrying out changing liquid, unlatching electromagnet makes partition board be moved to semicircular arc inside grooves,
Magnetotactic bacteria enters under the sucking action of electromagnet in semicircular arc groove, and magnetotactic bacteria is sucked away when can prevent from changing liquid, is carried
The culture efficiency of high bacterium.
Description of the drawings
The invention will be further described below in conjunction with the accompanying drawings.
Fig. 1 is the operational flowchart of the present invention；
Fig. 2 is the structural schematic diagram of culture device in the present invention；
Fig. 3 is that holder is mounted on the sectional view held in ware in the present invention；
Fig. 4 is partial enlarged view at the A of Fig. 2；
Fig. 5 is partial enlarged view at the B of Fig. 2；
Fig. 6 is the left view of roller in the present invention；
In figure：Babinet 1, shakes module 3, clamp module 4, liquid feeding module 5, take-up motor 31, take-up spring at case lid 2
32, roller 33, steel wire rope 34, support spring 35, hold ware 36, idler wheel 41, holder 42, electromagnet 43, No.1 spring 44, support
Block 45, culture dish 51, magnetic patch 52, upper layer liquid-feeding tube 53, gear 54, lower layer's liquid-feeding tube 56, No. two springs 6, fixture block 7, partition boards
8, No.1 spring 9, electromagnet 10.
Specific implementation mode
In order to make the technical means, the creative features, the aims and the efficiencies achieved by the present invention be easy to understand, tie below
Specific implementation mode is closed, the present invention is further explained.
As shown in Figures 1 to 6, a kind of magnetotactic bacteria cultural method of the present invention, this method are set using following culture
Standby, which includes babinet 1, case lid 2, shakes module 3, clamp module 4 and liquid feeding module 5, and the babinet 1 is rectangular
The left and right sidewall of shape uncovered babinet, babinet 1 symmetrically opens up left groove 11 and right groove 12；The case lid 2 is horizontally arranged at babinet
1 top；The inner bottom in babinet 1 is arranged in the shaking module 3, shakes module 3 for making magnetotactic bacteria rock to promote
Into incubation；The clamp module 4 is arranged in the top for shaking module 3, and clamp module 4 will be for that will hold magnetotactic bacteria
Device clamps, and improves device stability when shaking；The liquid feeding module 5 is arranged in the top of clamp module 4, and liquid feeding module 5 is used
In to inoculum is added inside babinet 1 to cultivate magnetotactic bacteria；
The cultural method includes the following steps：
Step 1：Open equipment：To babinet 1 is placed two in 30 DEG C of ± 1 DEG C of air-conditioned rooms after the progress sterilization treatment of babinet 1
A hour allows it to keep constant temperature；
Step 2：With liquid：The brine containing 3% salt is taken, and yeast powder, peptone and glycerine is added, adjusts PH to 7.0,130
DEG C sterilizing 20 minutes, K is added after sterilizing2HPO4Step is added in solution after being cooled to room temperature the magnetotactic bacteria culture solution configured
In culture dish 51 in a rapid middle case 1；
Step 3：It first passes through control clamp module 4 and clamps culture dish 51 stabilized, then take magnetotactic bacteria bacterium solution from upper layer
The center of liquid-feeding tube 53 is added in the culture dish 51 of step 2, controls inoculum and magnetotactic bacteria bacterium solution mixes 30 points
Clock；
Step 4：Open take-up motor 31, control shake module 3 to the inoculum in culture dish in step 3 51 into
Row rocks mixing, and inoculum and magnetotactic bacteria are sufficiently mixed；
Step 5：After culture solution in step 4 has been rocked a period of time, liquid feeding motor 55, control lower layer liquid feeding are opened
Pipe 56 enters in culture dish 51 to carry out changing liquid to the magnetotactic bacteria bacterium solution in culture dish 51, to promote magnetotactic in culture dish 51 thin
The reproductive process of bacterium.
The shaking module 3 includes take-up motor 31, take-up spring 32, roller 33, steel wire rope 34,35 and of support spring
Ware 36 is held, take-up motor 31 is installed in the left groove 11 of the babinet 1, take-up spring 32 is horizontally mounted in right groove 12；Institute
The center that roller 33 is placed on 1 bottom of babinet is stated, opens up No.1 groove on roller 33, lay winding wire ropes 34 in No.1 groove, steel
One end of cord 34 is connect with take-up motor 31, and the other end of steel wire rope 34 is connect with take-up spring 32；The support spring 35
Vertical to be connected to 1 bottom of babinet, support spring 35 is arranged two；The ware 36 that holds is placed on roller 33, is held outside ware 36
Layer bottom is arc, and inside is planar structure, holds 36 both ends of ware and is connect with the top of support spring 35, holds the interior of ware 36
Portion's level opens up No.1 sliding slot.When work, take-up motor 31 is opened, take-up motor 31 pulls steel wire rope 34, steel wire rope 34 to drive
Roller 33 moves left and right, and roller 33, which moves left and right drive and holds ware 36, to be moved left and right.
The clamp module 4 include idler wheel 41, holder 42, electromagnet 43, No.1 spring 44 and baffle 45, described one
Idler wheel 41 is installed, vertical connecting bracket 42 on idler wheel 41, holder 42 is L-type, and holder 42 is arranged two in number sliding slot；The branch
42 inner horizontal of frame opens up No. two grooves, is arranged electromagnet 43 in No. two grooves, 43 horizontal connection No.1 spring 44 of electromagnet,
The other end of No.1 spring 44 connects baffle 45.When work, electromagnet 43 is by driving No.1 spring 44 to drive baffle 45
Holder 42 is kept out on the side wall of No.1 sliding slot.
The liquid feeding module 5 includes culture dish 51, magnetic patch 52, upper layer liquid-feeding tube 53, gear 54, liquid feeding motor 55, lower layer
Liquid-feeding tube 56 and rack 57,42 centre of the holder connect culture dish 51, and culture dish 51 is rectangle uncovered babinet；The magnetic
Block 52 is mounted in the side wall of culture dish 51；The upper layer liquid-feeding tube 53 is vertically installed on case lid 2, and upper layer liquid-feeding tube 53 is
The bottom of cylindrical shape, upper layer liquid-feeding tube 53 is located at across case lid 2 inside babinet 1, and the lower half portion surface of upper layer liquid-feeding tube 53 is opened
If sliding slot；The gear 54 and liquid feeding motor 55 are arranged inside the entity of upper layer liquid-feeding tube 53；Lower layer's liquid-feeding tube 56 is set
It sets in the bottom of upper layer liquid-feeding tube 53, rack 57 is installed in 56 inside of lower layer's liquid-feeding tube, and rack 57 is meshed with gear 54, upper layer
Channel is opened up inside liquid-feeding tube 53 and lower layer's liquid-feeding tube 56.When work, liquid feeding motor 55 is opened, liquid feeding motor 55 passes through driving
Gear 54 rotates, and gear 54, to drive lower layer's liquid-feeding tube 56 to be moved upwardly or downwardly along sliding slot, is realized by band carry-over bar 57
The merging and separation of upper layer liquid-feeding tube 53 and lower layer's liquid-feeding tube 56, the meeting when there is liquid to be flowed into from the center of upper layer liquid-feeding tube 53
It is flowed into culture dish 51 along the center of lower layer's liquid-feeding tube 56, when needing to change liquid, by upper layer liquid-feeding tube 53 and lower layer's liquid feeding
The center of pipe 56 is blocked, and liquid is made to be flowed out from channel.
The other end of the holder No. two springs 6 of 42 horizontal connection, No. two springs 6 connects fixture block 7, fixture block 7 and culture dish
51 side walls connect.It is more steady that the setting of No. two springs 6 and fixture block 7 makes culture dish 51 be positioned to, and improves steady when equipment works
It is qualitative.
51 bottom of the culture dish opens up semicircular arc groove about central symmetry, and partition board 8, partition board is arranged in culture dish 51
8 are hinged on the side wall of semicircular arc groove, the vertical connection No.1 spring 9 in 8 lower section of partition board, 9 other end of No.1 spring and semicircle
Arc-shaped groove bottom connects；Electromagnet 10 is set inside 51 entity of the culture dish.When work, needed inside culture dish 51
When changing liquid, electromagnet 10 is opened by controlling to switch, electromagnet 10 attracts partition board to move downward, and magnetotactic bacteria enters semi arch
In connected in star, magnetotactic bacteria is gone out from upper layer liquid-feeding tube 53 and lower layer's liquid-feeding tube 56 when preventing from changing liquid, is changed after the completion of liquid, is disconnected
Control switch, electromagnet 10 lose magnetism, and magnetotactic bacteria returns in culture dish 51, and partition board 8 restores in the effect of No.1 spring 9
It is in situ.
When work, electromagnet 43 is first opened, electromagnet 43 is by driving No.1 spring 44 to drive baffle 45 by holder 42
It keeps out on the side wall of No.1 sliding slot.
Open liquid feeding motor 55, liquid feeding motor 55 by driving gear 54 to rotate, gear 54 by band carry-over bar 57 to
It drives lower layer liquid-feeding tube 56 to be moved downward along sliding slot, realizes the separation of upper layer liquid-feeding tube 53 and lower layer's liquid-feeding tube 56, liquid is from upper
The center of layer liquid-feeding tube 53 is flowed into along the center of lower layer's liquid-feeding tube 56 in culture dish 51 after flowing into.
So that upper layer liquid-feeding tube 53 and lower layer's liquid-feeding tube 56 is merged by controlling liquid feeding motor 55 again, open take-up motor 31,
Take-up motor 31 pulls steel wire rope 34, steel wire rope 34 that roller 33 is driven to move left and right, and roller 33 moves left and right drive and holds ware 36
It moves left and right, culture dish 51 follows to hold ware 36 and move left and right rock mixing to the culture solution in culture dish 51, accelerates thin
The incubation of bacterium.
When needing to change liquid, upper layer liquid-feeding tube 53 and lower layer's liquid-feeding tube 56 are separated, then by upper layer liquid-feeding tube 53 under
The center of layer liquid-feeding tube 56 is blocked, and the culture solution in culture dish 51 is logical inside upper layer liquid-feeding tube 53 and lower layer's liquid-feeding tube 56
Road outflow carries out liquid replacement, while opening electromagnet 10 by controlling to switch, and electromagnet 10 attracts partition board to move downward, becomes
Magnetic bacterium enters in semicircular arc groove, and magnetotactic bacteria is gone out from upper layer liquid-feeding tube 53 and lower layer's liquid-feeding tube 56 when preventing from changing liquid,
It changes after the completion of liquid, disconnects control switch, electromagnet 10 loses magnetism, and magnetotactic bacteria returns in culture dish 51, and partition board 8 is in No.1
The effect of spring 9 sets back.
The basic principles, main features and advantages of the invention have been shown and described above.The technical staff of the industry should
Understand, the present invention is not limited to the above embodiments, and the above embodiments and description only describe the originals of the present invention
Reason, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these change and change
Into all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by appended claims and its equivalent
Object defines.
Claims (6)
1. a kind of magnetotactic bacteria cultural method, it is characterised in that：The process employs culture device, which includes babinet
(1), case lid (2), shaking module (3), clamp module (4) and liquid feeding module (5), the babinet (1) are rectangle uncovered babinet,
The left and right sidewall of babinet (1) symmetrically opens up left groove (11) and right groove (12)；The case lid (2) is horizontally arranged at babinet (1)
Top；It is described to shake module (3) setting in the inner bottom of babinet (1), module (3) is shaken for making magnetotactic bacteria rock
To promote incubation；Clamp module (4) setting is in the top for shaking module (3), and clamp module (4) will be for that will hold
The device of magnetic bacterium clamps；In the top of clamp module (4), liquid feeding module (5) is used for babinet for liquid feeding module (5) setting
(1) internal that magnetotactic bacteria culture solution is added；
The cultural method includes the following steps：
Step 1：Open equipment：To babinet (1) is placed two in 30 DEG C of ± 1 DEG C of air-conditioned rooms after babinet (1) progress sterilization treatment
A hour allows it to keep constant temperature；
Step 2：With liquid：The brine containing 3% salt is taken, and yeast powder, peptone and glycerine is added, adjusts PH to 7.0,130 DEG C go out
Bacterium 20 minutes, is added K after sterilizing2HPO4Solution is added after being cooled to room temperature the magnetotactic bacteria culture solution configured in step 1
In culture dish in babinet (1)；
Step 3：It first passes through control clamp module (4) and clamps culture dish stabilized, then take bacterium solution from the center of upper layer liquid-feeding tube
It is added in the culture dish of step 2, controls magnetotactic bacteria culture solution and bacterium solution mixes 30 minutes；
Step 4：Take-up motor is opened, control shaking module (3) to the culture solution in culture dish in step 3 rock mixed
It closes；
Step 5：After culture solution in step 4 has been rocked a period of time, liquid feeding motor is opened, control liquid-feeding tube enters culture
The bacterium solution in culture dish is carried out in ware to change liquid.
2. a kind of magnetotactic bacteria cultural method according to claim 1, it is characterised in that：The shaking module (3) includes
Take-up motor (31), take-up spring (32), roller (33), steel wire rope (34), support spring (35) and hold ware (36), the case
Take-up motor (31) is installed in the left groove (11) of body (1), take-up spring (32) is horizontally mounted in right groove (12)；The roller
(33) it is placed on the center of babinet (1) bottom, opens up No.1 groove on roller (33), lay winding wire ropes (34) in No.1 groove,
One end of steel wire rope (34) is connect with take-up motor (31), and the other end of steel wire rope (34) is connect with take-up spring (32)；It is described
Support spring (35) is connected to babinet (1) bottom vertically, and support spring (35) is arranged two；The ware (36) that holds is placed on rolling
On cylinder (33), it is arc to hold ware (36) outer bottom, and inside is planar structure, holds ware (36) both ends and support spring (35)
Top connection, the inner horizontal for holding ware (36) opens up No.1 sliding slot.
3. a kind of magnetotactic bacteria cultural method according to claim 2, it is characterised in that：The clamp module (4) includes
Idler wheel (41), holder (42), electromagnet (43), No.1 spring (44) and baffle (45) install idler wheel in the No.1 sliding slot
(41), vertical connecting bracket (42) on idler wheel (41), holder (42) are L-type, and holder (42) is arranged two；In the holder (42)
Portion's level opens up No. two grooves, setting electromagnet (43) in No. two grooves, electromagnet (43) horizontal connection No.1 spring (44), and one
The other end connection baffle (45) of number spring (44).
4. a kind of magnetotactic bacteria cultural method according to claim 3, it is characterised in that：The liquid feeding module (5) includes
Culture dish (51), magnetic patch (52), upper layer liquid-feeding tube (53), gear (54), liquid feeding motor (55), lower layer's liquid-feeding tube (56) and rack
(57), connection culture dish (51) among the holder (42), culture dish (51) are rectangle uncovered babinet；Magnetic patch (52) peace
In the side wall of culture dish (51)；The upper layer liquid-feeding tube (53) is vertically installed on case lid (2), and upper layer liquid-feeding tube (53) is
The bottom of cylindrical shape, upper layer liquid-feeding tube (53) is located at babinet (1) inside, the lower half of upper layer liquid-feeding tube (53) across case lid (2)
Point surface opens up sliding slot；The gear (54) and liquid feeding motor (55) are arranged inside the entity of upper layer liquid-feeding tube (53)；It is described
Lower layer's liquid-feeding tube (56) setting installation rack (57), rack inside the bottom of upper layer liquid-feeding tube (53), lower layer's liquid-feeding tube (56)
(57) it is meshed with gear (54), channel is opened up inside upper layer liquid-feeding tube (53) and lower layer's liquid-feeding tube (56).
5. a kind of magnetotactic bacteria cultural method according to claim 3, it is characterised in that：Holder (42) horizontal connection
No. two springs (6), the other end connection fixture block (7) of No. two springs (6), fixture block (7) are connect with culture dish (51) side wall.
6. a kind of magnetotactic bacteria cultural method according to claim 4, it is characterised in that：It closes culture dish (51) bottom
Semicircular arc groove is opened up in central symmetry, partition board (8) is set in culture dish (51), partition board (8) is hinged on semicircular arc groove
Side wall on, vertical connection No.1 spring (9), No.1 spring (9) other end connect with semicircular arc bottom portion of groove below partition board (8)
It connects；Setting electromagnet (10) inside culture dish (51) entity.
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| CN201810283228.4A CN108467833A (en) | 2018-04-02 | 2018-04-02 | A kind of magnetotactic bacteria cultural method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109868209A (en) * | 2019-04-17 | 2019-06-11 | 重庆医药高等专科学校 | The evenly mixing device detected for bacterial number in food and medicine sample |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101230317A (en) * | 2008-02-27 | 2008-07-30 | 张小葵 | Culture device for magnetotactic bacteria |
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| CN109868209A (en) * | 2019-04-17 | 2019-06-11 | 重庆医药高等专科学校 | The evenly mixing device detected for bacterial number in food and medicine sample |
| CN109868209B (en) * | 2019-04-17 | 2022-04-15 | 重庆医药高等专科学校 | A mixing device that is arranged in food medicine to examine bacterium quantity in appearance to detect |
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