CN108464510A - A kind of collagen protein powder and preparation method thereof - Google Patents
A kind of collagen protein powder and preparation method thereof Download PDFInfo
- Publication number
- CN108464510A CN108464510A CN201810174026.6A CN201810174026A CN108464510A CN 108464510 A CN108464510 A CN 108464510A CN 201810174026 A CN201810174026 A CN 201810174026A CN 108464510 A CN108464510 A CN 108464510A
- Authority
- CN
- China
- Prior art keywords
- collagen
- parts
- fish
- bone
- powder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000008186 Collagen Human genes 0.000 title claims abstract description 64
- 108010035532 Collagen Proteins 0.000 title claims abstract description 64
- 239000000843 powder Substances 0.000 title claims abstract description 43
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 229920001436 collagen Polymers 0.000 claims abstract description 48
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 18
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 18
- 241000251468 Actinopterygii Species 0.000 claims abstract description 12
- 210000000988 bone and bone Anatomy 0.000 claims abstract description 10
- 241000239366 Euphausiacea Species 0.000 claims abstract description 8
- 239000005862 Whey Substances 0.000 claims abstract description 8
- 102000007544 Whey Proteins Human genes 0.000 claims abstract description 8
- 108010046377 Whey Proteins Proteins 0.000 claims abstract description 8
- CAXNYFPECZCGFK-UHFFFAOYSA-N 2-phenyl-2-pyridin-2-ylacetonitrile Chemical compound C=1C=CC=NC=1C(C#N)C1=CC=CC=C1 CAXNYFPECZCGFK-UHFFFAOYSA-N 0.000 claims abstract description 7
- 238000000034 method Methods 0.000 claims abstract description 5
- 239000000243 solution Substances 0.000 claims description 35
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 33
- 239000007788 liquid Substances 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 239000000203 mixture Substances 0.000 claims description 10
- 239000002002 slurry Substances 0.000 claims description 10
- 230000008961 swelling Effects 0.000 claims description 9
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 6
- 108090000526 Papain Proteins 0.000 claims description 6
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- 210000003195 fascia Anatomy 0.000 claims description 6
- 238000000889 atomisation Methods 0.000 claims description 5
- 238000000265 homogenisation Methods 0.000 claims description 5
- 238000007654 immersion Methods 0.000 claims description 5
- 239000013049 sediment Substances 0.000 claims description 5
- 238000005303 weighing Methods 0.000 claims description 5
- 239000004365 Protease Substances 0.000 claims description 4
- 238000001556 precipitation Methods 0.000 claims description 4
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 102000004190 Enzymes Human genes 0.000 claims description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 3
- 239000007983 Tris buffer Substances 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 229940088598 enzyme Drugs 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 3
- 230000007062 hydrolysis Effects 0.000 claims description 3
- 238000006460 hydrolysis reaction Methods 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 229940055729 papain Drugs 0.000 claims description 3
- 235000019834 papain Nutrition 0.000 claims description 3
- 150000003839 salts Chemical class 0.000 claims description 3
- 229910052708 sodium Inorganic materials 0.000 claims description 3
- 239000011734 sodium Substances 0.000 claims description 3
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- 239000008234 soft water Substances 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 3
- 238000010792 warming Methods 0.000 claims description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 2
- 239000008187 granular material Substances 0.000 claims description 2
- 239000001301 oxygen Substances 0.000 claims description 2
- 229910052760 oxygen Inorganic materials 0.000 claims description 2
- 238000002791 soaking Methods 0.000 claims description 2
- 235000010469 Glycine max Nutrition 0.000 claims 1
- 244000068988 Glycine max Species 0.000 claims 1
- 235000015165 citric acid Nutrition 0.000 claims 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid group Chemical class C(CC(O)(C(=O)O)CC(=O)O)(=O)O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims 1
- 238000012545 processing Methods 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 4
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 4
- 239000006227 byproduct Substances 0.000 abstract description 3
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 239000000654 additive Substances 0.000 abstract description 2
- 230000000996 additive effect Effects 0.000 abstract description 2
- 230000008131 children development Effects 0.000 abstract description 2
- 239000012535 impurity Substances 0.000 abstract description 2
- 238000009776 industrial production Methods 0.000 abstract description 2
- 239000004615 ingredient Substances 0.000 abstract description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 230000035764 nutrition Effects 0.000 abstract description 2
- 230000001737 promoting effect Effects 0.000 abstract description 2
- 239000000047 product Substances 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 3
- 244000131522 Citrus pyriformis Species 0.000 description 2
- 102000002734 Collagen Type VI Human genes 0.000 description 2
- 108010043741 Collagen Type VI Proteins 0.000 description 2
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 229910001882 dioxygen Inorganic materials 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 102000012422 Collagen Type I Human genes 0.000 description 1
- 108010022452 Collagen Type I Proteins 0.000 description 1
- 102000001187 Collagen Type III Human genes 0.000 description 1
- 108010069502 Collagen Type III Proteins 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- PMMYEEVYMWASQN-DMTCNVIQSA-N Hydroxyproline Chemical compound O[C@H]1CN[C@H](C(O)=O)C1 PMMYEEVYMWASQN-DMTCNVIQSA-N 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 101710172711 Structural protein Proteins 0.000 description 1
- 210000000845 cartilage Anatomy 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- PMMYEEVYMWASQN-UHFFFAOYSA-N dl-hydroxyproline Natural products OC1C[NH2+]C(C([O-])=O)C1 PMMYEEVYMWASQN-UHFFFAOYSA-N 0.000 description 1
- 102000034240 fibrous proteins Human genes 0.000 description 1
- 108091005899 fibrous proteins Proteins 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 229960002591 hydroxyproline Drugs 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000021332 multicellular organism growth Effects 0.000 description 1
- 239000002417 nutraceutical Substances 0.000 description 1
- 235000021436 nutraceutical agent Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 238000007634 remodeling Methods 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 210000002435 tendon Anatomy 0.000 description 1
- FGMPLJWBKKVCDB-UHFFFAOYSA-N trans-L-hydroxy-proline Natural products ON1CCCC1C(O)=O FGMPLJWBKKVCDB-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/10—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from hair, feathers, horn, skins, leather, bones, or the like
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/185—Vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/19—Dairy proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Mycology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The present invention relates to a kind of collagen protein powder, by following components according to being prepared：10 20 parts of fish bone collagen albumen powder, 10 20 parts of PURE WHEY, 15 25 parts of Fed Protein Powder of Pea Insteal, 10 20 parts of krill albumen powder, 50 60 parts of soyabean protein powder.Any additive is not added in the albumen powder of the present invention, nutrition is balanced, and dissolubility is good, absorption easy to digest, and protein content is high, contains phosphatide, the DHA isoreactivity ingredients for promoting infant development and increasing intelligence.The method for preparing collagen peptide of the present invention effectively can prepare collagen using fish processing byproduct fish-bone, of low cost, and removal of impurities and concentrated effect are good, be suitble to scale industrial production；The collagen free from extraneous odour of preparation.
Description
Technical field
The present invention relates to nutraceutical and its preparing technical field, more particularly to a kind of collagen protein powder and its preparation side
Method.
Background technology
Protein is the main matter for constituting human tissue organ, and highly important work is played in human life activity
With.It is necessary to maintaining and repairing body and cell growth, it not only influences the growth of body tissue, also participates in hormone
All various aspects such as generation, the maintenance of immune function, the transhipment of other nutriments and oxygen and the generation of hemoglobin.Letter speech
It, protein is one of the important nutrient for ensureing health.
Collagen is also known as collagen, is the fibrous proteins being mutually wound by three peptide chains, is a kind of glycoprotein,
Containing a large amount of glycine, proline, hydroxyproline and sugar, it is widely present in the tissues such as skin, bone, cartilage and the tendon of animal
In, it is the most class protein of body content.The collagen types having now found that be 19 kinds, wherein Type I collagen, II Collagen Type VI and
III Collagen Type VI is the main component for constituting collagenous fibres, is primarily present in the connective tissue of animal, rises and supports organ, protection machine
The function of body, is important structural protein.
China is aquatic products big country, and aquatic resources are abundant.China's processing of aquatic products is still based on roughing at present, intensive processing
The ratio of product is not high, and fish-bone is the by-product of fish processing, and protein content is very high, and the content of especially collagen accounts for
The half of its total protein is more.There is unique life using the small molecule collagen peptide that protease hydrolytic collagen obtains
Reason activity.
Invention content
For problems of the prior art, the object of the present invention is to provide a kind of collagen protein powder and its preparation sides
Method.
In order to solve the above-mentioned technical problem, the present invention is achieved by the following scheme：
A kind of collagen protein powder, by following components according to being prepared：
10-20 parts of fish bone collagen albumen powder, 10-20 parts of PURE WHEY, 15-25 parts of Fed Protein Powder of Pea Insteal, krill egg
10-20 parts of white powder, 50-60 parts of soyabean protein powder.
A kind of collagen protein powder, by following components according to being prepared：
15 parts of fish bone collagen albumen powder, 15 parts of PURE WHEY, 20 parts of Fed Protein Powder of Pea Insteal, 15 parts of krill albumen powder,
55 parts of soyabean protein powder.
A kind of preparation method of collagen protein powder as described above, is as follows：
(1) it is added in warm water and impregnates after weighing each component according to quantity；
(2) mixture after immersion is worn into slurry；
(3) slurry mixture carries out homogeneous, and homogenization pressure 20-23MPa, homogenizing temperature is between 55-65 DEG C；
(4) inlet air temperature control is at 130-140 DEG C, 85-90 DEG C of leaving air temp, degree of atomization 70-80%.
One kind extracting collagen preparation method from fish-bone, and step includes：
1) acetic acid solution that fish-bone is measured with 5 times, is stirred into extraction 72h at 5 DEG C, then uses refrigerated centrifuge with 3000r/
The speed of min centrifuges 10min, collects supernatant, and as collagen is swollen extracting solution；
2) it, is swollen in extracting solution in collagen and hydrogen peroxide is added, stand 5h after mixing, next use 80g/L lemons
Lemon three sodium solutions of acid adjust pH value and are equal to 5, and standing filters in a moment, abandons sediment, and the swelling that acquisition removes part foreign protein carries
Take liquid；
3) appropriate sodium chloride, is added into the swelling extracting solution under stiring, is slowly stirred, is stood at 4 DEG C after 10min
It centrifuges afterwards for 24 hours, collects salt precipitation object, the Tris buffer solutions for being 7.0 with pH value impregnate for 24 hours at 4 DEG C, centrifuge again, collect
Sediment is fitted into bag filter, with mass concentration be 1.2% acetic acid solution dialyse 18h after, then with distilled water dialyse 48h, most
What is obtained afterwards is the water-swellable liquid of the higher collagen of purity, spare after measurement wherein protein content；
4) soft water of 4 times of volumes, will be added in the water-swellable liquid of the high purity collagen of acquisition, be warming up to 55 under stiring
DEG C, it is 5 then to adjust solution ph with acetic acid, and papain is added and carries out 2~4h of enzyme hydrolysis, Papain enzyme dosage is glue
The 3% of the former water-swellable liquid protein quality of albumen obtains Hydrolyzed Collagen liquid；
5) hydrolyzate, is subjected to vacuum-concentrcted, is prepared into collagen liquid, the temperature of vacuum-concentrcted is 60~
70 DEG C, vacuum degree is -0.05~-0.04MPa.
On the basis of said program, the fish-bone in step 1) first removes fat and fascia.
On the basis of said program, the fish-bone after removal fat and fascia is broken into granule, molten with 0.5g/L sodium carbonate
Liquid impregnates 1h, is dried after cleaning 2 times later.
On the basis of said program, the acetic acid solution a concentration of 0.5% in step 1).
On the basis of said program, the dioxygen water consumption in step 2) is that its body is added in collagen swelling extracting solution
Product 0.5%.
Any additive is not added in the albumen powder of the present invention, nutrition is balanced, and dissolubility is good, absorption easy to digest, protein
Content is high, contains phosphatide, the DHA isoreactivity ingredients for promoting infant development and increase intelligence.The present invention's prepares collagen peptide
Method effectively can prepare collagen using fish processing byproduct fish-bone, of low cost, and removal of impurities and concentrated effect are good, fit
Close scale industrial production；The collagen free from extraneous odour of preparation.
Specific implementation mode
Below in conjunction with preferred embodiment, to the specific implementation mode that provides according to the present invention, details are as follows：
Embodiment 1
One kind extracting collagen preparation method from fish-bone, and step includes：
1) acetic acid solution that fish-bone is measured with 5 times, is stirred into extraction 72h at 5 DEG C, then uses refrigerated centrifuge with 3000r/
The speed of min centrifuges 10min, collects supernatant, and as collagen is swollen extracting solution；
2) it, is swollen in extracting solution in collagen and hydrogen peroxide is added, stand 5h after mixing, next use 80g/L lemons
Lemon three sodium solutions of acid adjust pH value and are equal to 5, and standing filters in a moment, abandons sediment, and the swelling that acquisition removes part foreign protein carries
Take liquid；
3) appropriate sodium chloride, is added into the swelling extracting solution under stiring, is slowly stirred, is stood at 4 DEG C after 10min
It centrifuges afterwards for 24 hours, collects salt precipitation object, the Tris buffer solutions for being 7.0 with pH value impregnate for 24 hours at 4 DEG C, centrifuge again, collect
Sediment is fitted into bag filter, with mass concentration be 1.2% acetic acid solution dialyse 18h after, then with distilled water dialyse 48h, most
What is obtained afterwards is the water-swellable liquid of the higher collagen of purity, spare after measurement wherein protein content；
4) soft water of 4 times of volumes, will be added in the water-swellable liquid of the high purity collagen of acquisition, be warming up to 55 under stiring
DEG C, it is 5 then to adjust solution ph with acetic acid, and papain is added and carries out 2~4h of enzyme hydrolysis, Papain enzyme dosage is glue
The 3% of the former water-swellable liquid protein quality of albumen obtains Hydrolyzed Collagen liquid；
5) hydrolyzate, is subjected to vacuum-concentrcted, is prepared into collagen liquid, the temperature of vacuum-concentrcted is 60~
70 DEG C, vacuum degree is -0.05~-0.04MPa.
Preferably, the fish-bone in step 1) first removes fat and fascia.Fish-bone after removal fat and fascia is broken into small
Grain is dried with 0.5g/L soaking in sodium carbonate solution 1h after cleaning 2 times later.
On the basis of said program, the acetic acid solution a concentration of 0.5% in step 1).
On the basis of said program, the dioxygen water consumption in step 2) is that its body is added in collagen swelling extracting solution
Product 0.5%.
Embodiment 2
A kind of collagen protein powder, by following components according to being prepared：
10 parts of fish bone collagen albumen powder, 10 parts of PURE WHEY, 15 parts of Fed Protein Powder of Pea Insteal, 10 parts of krill albumen powder,
50 parts of soyabean protein powder.
Preparation method is as follows：
(1) it is added in warm water and impregnates after weighing each component according to quantity；
(2) mixture after immersion is worn into slurry；
(3) slurry mixture carries out homogeneous, and homogenization pressure 20-23MPa, homogenizing temperature is between 55-65 DEG C；
(4) inlet air temperature control is at 130-140 DEG C, 85-90 DEG C of leaving air temp, degree of atomization 70-80%.
Embodiment 3
A kind of collagen protein powder, by following components according to being prepared：
15 parts of fish bone collagen albumen powder, 15 parts of PURE WHEY, 20 parts of Fed Protein Powder of Pea Insteal, 15 parts of krill albumen powder,
55 parts of soyabean protein powder.
Preparation method is as follows：
(1) it is added in warm water and impregnates after weighing each component according to quantity；
(2) mixture after immersion is worn into slurry；
(3) slurry mixture carries out homogeneous, and homogenization pressure 20-23MPa, homogenizing temperature is between 55-65 DEG C；
(4) inlet air temperature control is at 130-140 DEG C, 85-90 DEG C of leaving air temp, degree of atomization 70-80%.
Embodiment 4
A kind of collagen protein powder, by following components according to being prepared：
20 parts of fish bone collagen albumen powder, 20 parts of PURE WHEY, 25 parts of Fed Protein Powder of Pea Insteal, 20 parts of krill albumen powder,
60 parts of soyabean protein powder.
Preparation method is as follows：
(1) it is added in warm water and impregnates after weighing each component according to quantity；
(2) mixture after immersion is worn into slurry；
(3) slurry mixture carries out homogeneous, and homogenization pressure 20-23MPa, homogenizing temperature is between 55-65 DEG C；
(4) inlet air temperature control is at 130-140 DEG C, 85-90 DEG C of leaving air temp, degree of atomization 70-80%.
The above described is only a preferred embodiment of the present invention, being not that the invention has other forms of limitations, appoint
What those skilled in the art changed or be modified as possibly also with the technology contents of the disclosure above equivalent variations etc.
Imitate embodiment.But it is every without departing from technical solution of the present invention content, according to the technical essence of the invention to above example institute
Any simple modification, equivalent variations and the remodeling made, still fall within the protection domain of technical solution of the present invention.
Claims (8)
1. a kind of collagen protein powder, which is characterized in that by following components according to being prepared：
10-20 parts of fish bone collagen albumen powder, 10-20 parts of PURE WHEY, 15-25 parts of Fed Protein Powder of Pea Insteal, krill albumen powder
10-20 parts, 50-60 parts of soyabean protein powder.
2. collagen protein powder according to claim 1, which is characterized in that by following components according to being prepared：
15 parts of fish bone collagen albumen powder, 15 parts of PURE WHEY, 20 parts of Fed Protein Powder of Pea Insteal, 15 parts of krill albumen powder, soybean
55 parts of albumen powder.
3. a kind of preparation method of collagen protein powder as claimed in claim 1 or 2, which is characterized in that be as follows：
(1) it is added in warm water and impregnates after weighing each component according to quantity；
(2) mixture after immersion is worn into slurry；
(3) slurry mixture carries out homogeneous, and homogenization pressure 20-23MPa, homogenizing temperature is between 55-65 DEG C；
(4) inlet air temperature control is at 130-140 DEG C, 85-90 DEG C of leaving air temp, degree of atomization 70-80%.
4. collagen protein powder according to claim 1 or 2, which is characterized in that the preparation side of the fish bone collagen albumen
Method is as follows：
1) acetic acid solution that fish-bone is measured with 5 times, is stirred into extraction 72h at 5 DEG C, then uses refrigerated centrifuge with 3000r/min
Speed centrifuge 10min, collect supernatant, as collagen be swollen extracting solution；
2) it, is swollen in extracting solution in collagen and hydrogen peroxide is added, stand 5h after mixing, next use 80g/L citric acids
Three sodium solutions adjust pH value and are equal to 5, and standing filters in a moment, abandons sediment, obtains the swelling extracting solution for removing part foreign protein；
3) appropriate sodium chloride, is added into the swelling extracting solution under stiring, is slowly stirred, is stood for 24 hours at 4 DEG C after 10min
After centrifuge, collect salt precipitation object, with pH value be 7.0 Tris buffer solutions impregnated for 24 hours at 4 DEG C, centrifuge again, collect precipitation
Object is fitted into bag filter, and the acetic acid solution for being 1.2% with mass concentration is dialysed after 18h, then is dialysed 48h with distilled water, finally
To be the water-swellable liquid of the higher collagen of purity, measure spare wherein after protein content；
4) soft water of 4 times of volumes, will be added in the water-swellable liquid of the high purity collagen of acquisition, be warming up to 55 DEG C under stiring,
Then it is 5 to adjust solution ph with acetic acid, and papain is added and carries out 2~4h of enzyme hydrolysis, Papain enzyme dosage is collagen
The 3% of the water-swellable liquid protein quality of albumen obtains Hydrolyzed Collagen liquid；
5) hydrolyzate, is subjected to vacuum-concentrcted, is prepared into collagen liquid, the temperature of vacuum-concentrcted is 60~70
DEG C, vacuum degree is -0.05~-0.04MPa.
5. collagen protein powder according to claim 4, which is characterized in that
Fish-bone in step 1) first removes fat and fascia.
6. according to claim 5 extract collagen preparation method from fish-bone, which is characterized in that
Fish-bone after removal fat and fascia is broken into granule, with 0.5g/L soaking in sodium carbonate solution 1h, dries in the air after cleaning 2 times later
It is dry.
7. according to claim 6 extract collagen preparation method from fish-bone, which is characterized in that the second in step 1)
Acid solutions are 0.5%.
8. according to claim 7 extract collagen preparation method from fish-bone, which is characterized in that double in step 2)
Oxygen water consumption is that its volume 0.5% is added in collagen swelling extracting solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810174026.6A CN108464510A (en) | 2018-03-02 | 2018-03-02 | A kind of collagen protein powder and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810174026.6A CN108464510A (en) | 2018-03-02 | 2018-03-02 | A kind of collagen protein powder and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108464510A true CN108464510A (en) | 2018-08-31 |
Family
ID=63264985
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810174026.6A Pending CN108464510A (en) | 2018-03-02 | 2018-03-02 | A kind of collagen protein powder and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108464510A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109567210A (en) * | 2019-01-04 | 2019-04-05 | 广东海洋大学 | A kind of Fish protein and vegetable protein or lactoprotein composite powder |
| CN115590202A (en) * | 2022-10-24 | 2023-01-13 | 东北农业大学（Cn） | Nutritional powder beneficial to absorption of soybean protein and preparation method thereof |
-
2018
- 2018-03-02 CN CN201810174026.6A patent/CN108464510A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109567210A (en) * | 2019-01-04 | 2019-04-05 | 广东海洋大学 | A kind of Fish protein and vegetable protein or lactoprotein composite powder |
| CN115590202A (en) * | 2022-10-24 | 2023-01-13 | 东北农业大学（Cn） | Nutritional powder beneficial to absorption of soybean protein and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102690853B (en) | Preparation method of sleeve-fish skin low-molecular-weight collagen peptide | |
| CN103789381B (en) | A kind of method of Isin glue collagen rapid extraction | |
| CN107236777A (en) | The method that collagen is extracted from crucian fish-skin | |
| CN106359631B (en) | A kind of pure collagen milk powder and preparation method thereof | |
| CN103386144B (en) | Preparation method of fish collagen combined chitosan biological dressing | |
| CN109180808A (en) | A kind of fish scale collagen and its preparation method and application | |
| CN106333276B (en) | A kind of dried eggs and its processing technology | |
| CN107365824A (en) | A kind of preparation method for hydrolyzing Isin glue collagen Gly-His-Lys | |
| CN107198252B (en) | Silkworm pupa protein, compound protein thereof and preparation method thereof | |
| CN104585790A (en) | Preparation method of nutritious cumquat and fish scale jelly | |
| CN105614863A (en) | Processing process of premna microphylla leaf emerald bean jellies | |
| CN108727485A (en) | A kind of preparation method of food-grade hydrolysis of keratin | |
| CN108464510A (en) | A kind of collagen protein powder and preparation method thereof | |
| Suo-Lian et al. | Technology for extracting effective components from fish scale | |
| CN101928744A (en) | Process for extracting active collagen peptide from salmon trout waste | |
| CN101965982A (en) | Method for preparing beverage from silk peptide | |
| CN108669549A (en) | A kind of nutrition albumen powder and preparation method thereof | |
| CN108410931A (en) | A method of producing fish skin and scale collagen | |
| CN104472848A (en) | Method for preparing low-value seashell oligopeptide based on simulated digestion | |
| CN107252060A (en) | A kind of processing method of low value eupleurogrammus muticus fish meal | |
| CN108277252A (en) | One kind extracting collagen preparation method from fish-bone | |
| CN108277250A (en) | A method of preparing collagen using fish-bone | |
| JP2023530376A (en) | Method for producing collagen peptide | |
| CN113122604A (en) | Sea intestine glue and preparation method and application thereof | |
| CN102031279A (en) | Method for extracting ultralow molecular collagen |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180831 |