CN108445262A - A kind of scanning microsphere lens super-resolution microscope and its imaging method based near field optic - Google Patents
A kind of scanning microsphere lens super-resolution microscope and its imaging method based near field optic Download PDFInfo
- Publication number
- CN108445262A CN108445262A CN201810184274.9A CN201810184274A CN108445262A CN 108445262 A CN108445262 A CN 108445262A CN 201810184274 A CN201810184274 A CN 201810184274A CN 108445262 A CN108445262 A CN 108445262A
- Authority
- CN
- China
- Prior art keywords
- scanning
- microsphere lens
- near field
- super
- based near
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01Q—SCANNING-PROBE TECHNIQUES OR APPARATUS; APPLICATIONS OF SCANNING-PROBE TECHNIQUES, e.g. SCANNING PROBE MICROSCOPY [SPM]
- G01Q60/00—Particular types of SPM [Scanning Probe Microscopy] or microscopes; Essential components thereof
- G01Q60/18—SNOM [Scanning Near-Field Optical Microscopy] or apparatus therefor, e.g. SNOM probes
- G01Q60/22—Probes, their manufacture, or their related instrumentation, e.g. holders
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01Q—SCANNING-PROBE TECHNIQUES OR APPARATUS; APPLICATIONS OF SCANNING-PROBE TECHNIQUES, e.g. SCANNING PROBE MICROSCOPY [SPM]
- G01Q30/00—Auxiliary means serving to assist or improve the scanning probe techniques or apparatus, e.g. display or data processing devices
- G01Q30/08—Means for establishing or regulating a desired environmental condition within a sample chamber
- G01Q30/12—Fluid environment
- G01Q30/14—Liquid environment
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01Q—SCANNING-PROBE TECHNIQUES OR APPARATUS; APPLICATIONS OF SCANNING-PROBE TECHNIQUES, e.g. SCANNING PROBE MICROSCOPY [SPM]
- G01Q60/00—Particular types of SPM [Scanning Probe Microscopy] or microscopes; Essential components thereof
- G01Q60/02—Multiple-type SPM, i.e. involving more than one SPM techniques
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01Q—SCANNING-PROBE TECHNIQUES OR APPARATUS; APPLICATIONS OF SCANNING-PROBE TECHNIQUES, e.g. SCANNING PROBE MICROSCOPY [SPM]
- G01Q60/00—Particular types of SPM [Scanning Probe Microscopy] or microscopes; Essential components thereof
- G01Q60/18—SNOM [Scanning Near-Field Optical Microscopy] or apparatus therefor, e.g. SNOM probes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01Q—SCANNING-PROBE TECHNIQUES OR APPARATUS; APPLICATIONS OF SCANNING-PROBE TECHNIQUES, e.g. SCANNING PROBE MICROSCOPY [SPM]
- G01Q60/00—Particular types of SPM [Scanning Probe Microscopy] or microscopes; Essential components thereof
- G01Q60/44—SICM [Scanning Ion-Conductance Microscopy] or apparatus therefor, e.g. SICM probes
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Radiology & Medical Imaging (AREA)
- Length Measuring Devices By Optical Means (AREA)
Abstract
The invention discloses a kind of scanning microsphere lens super-resolution microscopes based near field optic for liquid environment, including：Light microscope, microsphere lens, container, glass tube probe, sample electrodes, grounding electrode, DC power supply, current amplifier, Real-time Feedback and scanning monitor and what can be moved on three-dimensional be pre-adjusted displacement platform and scan table, the anode and sample electrodes of DC power supply are connected with the respective end of current amplifier respectively, and the glass tube probe includes：Be pre-adjusted the flat segments that displacement platform is connected, oblique section be connected with flat segments, oblique section of bottom end is provided with needle tip, and sample electrodes are placed in glass tube probe, and the needle tip at oblique section is arranged in microsphere lens, and vertex is suitable with the bottom of microsphere lens.Scanning microsphere lens super-resolution microscope of the present invention based near field optic is mainly used for the fields such as the life science for needing nanoscale super-resolution dynamically to observe and operate in real time in liquid environment.
Description
Technical field
The present invention relates to a kind of optical ultra-discrimination rate based on scanning Ion Conductance Microscope and microsphere lens is a wide range of
Scanning imaging system is mainly used for the life science for needing nanoscale super-resolution dynamically to observe and operate in real time in liquid environment
Equal fields.
Background technology
Late nineteenth century, Germany scientist Ernest ＆ Whitney Abbe define the resolution ratio of light microscope, it is believed that are light waves
Long half, i.e.,：About 0.2 micron, here it is famous optical diffraction limits.Therefore, in the most of the time of twentieth century
In, scientists, which all believe light microscope forever, can not break through the limitation of diffraction limit.However, with the depth of scientific research
Enter, especially the research of bio-science field, people begin one's study the internal structure of cell already, it is therefore desirable to cell interior
Institutional framework carry out vivo observation.Electron microscope can only observe the surface of dead cell, can not observe the interior of active somatic cell
Portion's structure.Not long ago, researcher had found that microsphere lens can realize that the optical imagery of super-resolution, technical research results are delivered earliest
In on Nature magazines in 2009 (www.nature.com/doifinder/10.1038/nature08173), point at that time
Resolution is 220nm.After 2 years, this resolution ratio has been raised to 50nm (Nature Communication, DOI:
10.1038/ncomms1211).2012, researcher had found again, and not only the super-resolution imaging of microsphere lens can be in air
Middle realization, but also can realize in a liquid, and can reach and be imaged identical resolution ratio in air
(Appl.Phys.Lett.101,141128；https://doi.org/10.1063/1.4757600).Different from fluorescence microscopy
Mirror, the nanometer imaging based on microsphere lens do not need fluorescein stain, and are to be suitble to any sample under the conditions of natural lighting
Product are imaged, thus have more widely applied meaning.
Currently, being combined by the feedback control mechanism with atomic force microscope, make microsphere lens and sample product surface
Distance is accurately controlled, and a wide range of dynamic scan super-resolution imaging (Nature is carried out to sample surfaces to realize
Communication,DOI:10.1038/ncomms13748).But such feedback mechanism is only suitable for being imaged in air, and
System light path itself is complicated, and cost is very expensive, cannot be applied in liquid environment.It is effective due to lacking in liquid environment
Feedback control means accurately control the distance of microsphere lens and sample surfaces, at present the super-resolution based on microsphere lens in liquid
Imaging system can only generally be imaged in fixed position, not have any position and large area scanning imaging capability.
Invention content
The technical problem to be solved by the present invention is to：There is provided it is a kind of can be applied to liquid environment based near field optic
Microsphere lens super-resolution microscope is scanned, large area scanning imaging can be carried out in any position of sample surfaces.
In order to solve the above technical problems, the technical solution adopted by the present invention is：A kind of scanning microballoon based near field optic
Lens super-resolution microscope, structure include：Light microscope, microsphere lens, the container for placing sample, glass tube are visited
Needle, sample electrodes, grounding electrode, DC power supply and current amplifier, moved on three-dimensional be pre-adjusted displacement platform and
Scan table and the Real-time Feedback moved come real-time control scan table according to the feedback signal of current amplifier and scan control
Device, the light microscope are located at the top of microsphere lens, the anode and sample electrodes of the DC power supply respectively with it is described
The respective end of current amplifier is connected, and the output end of current amplifier is connected with Real-time Feedback and scanning monitor, DC power supply
Cathode be connected with grounding electrode, the concrete structure of the glass tube probe includes：It is pre-adjusted displacement platform with described
Connected flat segments, oblique section to be connected with flat segments, oblique section of bottom end are provided with needle tip, the apex setting of needle tip
There are opening, the sample electrodes to be placed in glass tube probe, the grounding electrode is placed in the container, close to container
Bottom, the microsphere lens are arranged in the side of oblique section of needle tip, in vertical direction, the vertex of the needle tip with
The bottom of the microsphere lens is suitable；It is provided with identical conducting solution in the container and glass tube probe.
As a preferred embodiment, in a kind of scanning microsphere lens super-resolution microscope based near field optic
In, in vertical direction, the vertex of needle tip is flushed with the bottom of the microsphere lens.
As a preferred embodiment, in a kind of scanning microsphere lens super-resolution microscope based near field optic
In, the conducting solution is phosphate buffered saline solution.
As a preferred embodiment, in a kind of scanning microsphere lens super-resolution microscope based near field optic
In, the opening of the needle tip apex is less than 100 nanometers.
As a preferred embodiment, in a kind of scanning microsphere lens super-resolution microscope based near field optic
In, the sample electrodes and grounding electrode are Ag/AgCl electrodes.
The imaging method for the scanning microsphere lens super-resolution microscope that the present invention also provides a kind of based near field optic,
Its step is：When scanning imagery, the microsphere lens is fixed relative to the position of light microscope, by scan table with
Sample in container and container makees scanning motion relative to microsphere lens together.
As a preferred embodiment, in a kind of scanning microsphere lens super-resolution microscope based near field optic
Imaging method in, when scanning imagery, the movement of scan table is controlled by Real-time Feedback and scanning monitor so that microballoon is saturating
The distance between the bottom of mirror and sample surfaces are consistently less than the size of the needle tip apex opening of glass tube probe.
The present invention flows through the electric current of glass tube probe by detection, utilizes the feedback similar with scanning Ion Conductance Microscope
Control principle controls the open-mouth ruler that microballoon bottom is less than probe tips at a distance from sample surfaces always in scanning process
It is very little, to meet the super-resolution imaging condition of microsphere lens；It, can be real by the imaging joint to adjacent area in scanning process
Existing optical ultra-discrimination rate is imaged on a large scale.
The beneficial effects of the invention are as follows：
1, the present invention is combined by microsphere lens with ordinary optical microscope, has broken optical diffraction limit, realizes oversubscription
Resolution optical imagery；By using the feedback control principle of surface sweeping Ion Conductance Microscope, realize under liquid environment to microballoon
Position accurately controls, and realizes the optical ultra-discrimination rate large area scanning imaging to sample surfaces any position.
2, present invention employs by the container, glass tube probe, sample electrodes, grounding electrode, DC power supply, electric current
The position feedback control device for the scanning Ion Conductance Microscope that amplifier and real-time feedback control device are constituted, it is aobvious to eliminate atomic force
The control system and light path system of micro mirror complex and expensive, greatly reduce manufacturing cost.
Description of the drawings
Fig. 1 is the principle of the present invention structural schematic diagram.
Reference numeral in Fig. 1 is：1, light microscope, 2, be pre-adjusted displacement platform, 3, container, 4, microsphere lens, 5,
Sample, 6, glass tube probe, 61, flat segments, 62, oblique section, 621, needle tip, 7, sample electrodes, 8, grounding electrode, 9, electricity
Stream amplifier, 10, DC power supply, 11, scan table, 12, Real-time Feedback and scanning monitor.
Specific implementation mode
Below in conjunction with the accompanying drawings, a kind of scanning microsphere lens super-resolution based near field optic of the present invention is described in detail
The specific embodiment of rate microscope and its imaging method：
As shown in Figure 1, a kind of scanning microsphere lens super-resolution microscope based near field optic of the present invention, packet
It includes：Light microscope 1, microsphere lens 4, the container 3 for placing sample 5, glass tube probe 6, sample electrodes 7, grounding electrode
8, DC power supply 10 and current amplifier 9, moved on three-dimensional be pre-adjusted displacement platform 2 and scan table 11, Yi Jigen
Come the Real-time Feedback and scanning monitor 12 of the movement of real-time control scan table 11 according to the feedback signal of current amplifier 9, optics is aobvious
Micro mirror 1 is located at the top of microsphere lens 4, the anode and sample electrodes 7 of the DC power supply 10 respectively with the current amplifier 9
Respective end be connected, the output end of current amplifier 9 is connected with Real-time Feedback and scanning monitor 12, the DC power supply 10
Cathode be connected with grounding electrode 8, the concrete structure of the glass tube probe 6 includes：It is pre-adjusted displacement with described
Platform 2 connected flat segments 61, be connected with flat segments 61 oblique section 62, oblique section 62 of bottom end is provided with needle tip 621, needle point
The apex in portion 621 offers the opening that size is less than or equal to 100 nanometers, and the sample electrodes 7 are placed in glass tube probe 6
In oblique section 62, the grounding electrode 8 is placed in the container 3, close to the bottom of container 3, the microsphere lens 4
It is arranged in the side of oblique section 62 of needle tip 621, in vertical direction, oblique section 62 of needle tip 621 of glass tube probe 6
Vertex it is suitable with the bottom of the microsphere lens 4；In the present embodiment, the sample electrodes 7 and grounding electrode 8 are Ag/
AgCl electrodes.
When practical application, entire oblique section 62 of glass tube probe 6 is made coniform, oblique section 62 of bottom end formation needle
Tip 621；In vertical direction, the vertex of the needle tip 621 is usually flushed with the bottom of the microsphere lens 4；It is described
Glass tube probe 6 be ultra micro glass tube probe, i.e.,：Its internal diameter in this application, is opened usually in nanometer between submicron order
Mouth size should be less than 100 nanometers.
The imaging method of scanning microsphere lens super-resolution microscope of the present invention based near field optic is：First
It is pre-adjusted displacement platform 2 by manual adjustment, by microsphere lens 4 close to sample 5, then, connects DC power supply 10, DC power supply
10 apply certain voltage on sample electrodes 7 and grounding electrode 8, in this way, flowing through the ionic current of glass tube probe 6 with glass
Oblique section 62 of needle tip 621 of glass pipe probe 6 changes to the distance between sample 5 and is changed, and current amplifier 9 is by electric current
Variation be converted into feedback signal and send Real-time Feedback and scanning monitor 12 to, it is real-time by Real-time Feedback and scanning monitor 12
The movement for adjusting scan table 11, in this way, scan table 11 is just saturating relative to microballoon together with the sample 5 in container 3 and container 3
Mirror 4 makees scanning motion, kept in scanning process the microsphere lens 4 on glass tube probe 6 between sample 5 at a distance from it is constant,
Realize large area scanning imaging.
In conclusion only presently preferred embodiments of the present invention, is not used for limiting the scope of implementation of the present invention, it is all according to
Equivalent changes and modifications made by shape, construction, feature and spirit described in scope of the invention as claimed should all be included in this
In the right of invention.
Claims (7)
1. a kind of scanning microsphere lens super-resolution microscope based near field optic, including：Light microscope (1), microballoon are saturating
Mirror (4) and what can be moved on three-dimensional be pre-adjusted displacement platform (2), it is saturating that the light microscope (1) is located at microballoon
The top of mirror (4), it is characterised in that：The scanning microsphere lens super-resolution microscope based near field optic further includes
Have：Container (3), glass tube probe (6), sample electrodes (7), grounding electrode (8), DC power supply for placing sample (5)
(10), current amplifier (9), the scan table (11) that is moved on three-dimensional and the feedback letter according to current amplifier (9)
Number come the mobile Real-time Feedback of scan table described in real-time control (11) and scanning monitor (12), the DC power supply (10) is just
Pole and sample electrodes (7) are connected with the respective end of the current amplifier (9) respectively, the output end and reality of current amplifier (9)
When feedback be connected with scanning monitor (12), the cathode of the DC power supply (10) is connected with grounding electrode (8), glass tube probe
(6) concrete structure includes：With it is described be pre-adjusted flat segments (61) that displacement platform (2) is connected and with flat segments (61)
The bottom end of oblique section connected (62), oblique section (62) is provided with needle tip (621), and the apex of needle tip (621) is provided with
Opening, the sample electrodes (7) are placed in glass tube probe (6), the grounding electrode (8) is placed in the container (3),
Close to the bottom of container (3), the microsphere lens (4) setting is in the side of the needle tip (621) of oblique section (62), vertical
On direction, the vertex of the needle tip (621) is suitable with the bottom of the microsphere lens (4)；The container (3) and glass tube
Probe is provided with identical conducting solution in (6).
2. a kind of scanning microsphere lens super-resolution microscope based near field optic according to claim 1, feature
It is：In vertical direction, the vertex of the needle tip (621) is flushed with the bottom of the microsphere lens (4).
3. a kind of scanning microsphere lens super-resolution microscope based near field optic according to claim 1, feature
It is：The opening of needle tip (621) apex is less than 100 nanometers.
4. a kind of scanning microsphere lens super-resolution microscope based near field optic according to claim 1, feature
It is：The conducting solution is phosphate buffered saline solution.
5. a kind of scanning microsphere lens super-resolution based near field optic according to any one of claim 1 to 4 is aobvious
Micro mirror, it is characterised in that：The sample electrodes (7) and grounding electrode (8) are Ag/AgCl electrodes.
6. a kind of imaging method of the scanning microsphere lens super-resolution microscope described in claim 1 based near field optic,
Its step is：When scanning imagery, the microsphere lens (4) is fixed relative to the position of light microscope (1), by scanning
Platform (11) makees scanning motion relative to microsphere lens (4) together with the sample (5) in container (3) and container (3).
7. the imaging method of the scanning microsphere lens super-resolution microscope according to claim 6 based near field optic,
It is characterized in that：When scanning imagery, the movement of scan table (11) is controlled by Real-time Feedback and scanning monitor so that microballoon
The distance between bottom and sample (5) surface of lens (4) are consistently less than needle tip (621) apex of glass tube probe (6)
The size of opening.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810184274.9A CN108445262A (en) | 2018-03-06 | 2018-03-06 | A kind of scanning microsphere lens super-resolution microscope and its imaging method based near field optic |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810184274.9A CN108445262A (en) | 2018-03-06 | 2018-03-06 | A kind of scanning microsphere lens super-resolution microscope and its imaging method based near field optic |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108445262A true CN108445262A (en) | 2018-08-24 |
Family
ID=63193343
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810184274.9A Pending CN108445262A (en) | 2018-03-06 | 2018-03-06 | A kind of scanning microsphere lens super-resolution microscope and its imaging method based near field optic |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108445262A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111381355A (en) * | 2018-12-29 | 2020-07-07 | 南京培轩雅谱光电科技有限公司 | Optical imaging apparatus and method |
| CN112653834A (en) * | 2020-12-01 | 2021-04-13 | 广东鼎诚电子科技有限公司 | Super-resolution scanning imaging method, system and storage medium |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000063736A2 (en) * | 1999-04-19 | 2000-10-26 | Imperial College Innovations Limited | Optical microscopy and its use in the study of cells |
| CN101430321A (en) * | 2008-12-12 | 2009-05-13 | 国家纳米技术与工程研究院 | Non-contact biological detection scanning nano-glass probe microscope and its operation method |
| CN102305776A (en) * | 2011-05-26 | 2012-01-04 | 浙江大学 | Transparent-medium-microsphere-based super-resolution microscopic imaging system |
| CN105988021A (en) * | 2015-02-05 | 2016-10-05 | 中国科学院沈阳自动化研究所 | Optical super-resolution dynamic imaging system and method based on microlens modified probe |
| CN106940470A (en) * | 2017-05-04 | 2017-07-11 | 苏州大学 | Optical ultra-discrimination fast imaging device and imaging method |
| CN107402443A (en) * | 2017-08-08 | 2017-11-28 | 苏州显纳精密仪器有限公司 | A kind of optical ultra-discrimination rate imaging system based on inverted microscope and microsphere lens and the dynamic imaging methods using the system |
-
2018
- 2018-03-06 CN CN201810184274.9A patent/CN108445262A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000063736A2 (en) * | 1999-04-19 | 2000-10-26 | Imperial College Innovations Limited | Optical microscopy and its use in the study of cells |
| CN101430321A (en) * | 2008-12-12 | 2009-05-13 | 国家纳米技术与工程研究院 | Non-contact biological detection scanning nano-glass probe microscope and its operation method |
| CN102305776A (en) * | 2011-05-26 | 2012-01-04 | 浙江大学 | Transparent-medium-microsphere-based super-resolution microscopic imaging system |
| CN105988021A (en) * | 2015-02-05 | 2016-10-05 | 中国科学院沈阳自动化研究所 | Optical super-resolution dynamic imaging system and method based on microlens modified probe |
| CN106940470A (en) * | 2017-05-04 | 2017-07-11 | 苏州大学 | Optical ultra-discrimination fast imaging device and imaging method |
| CN107402443A (en) * | 2017-08-08 | 2017-11-28 | 苏州显纳精密仪器有限公司 | A kind of optical ultra-discrimination rate imaging system based on inverted microscope and microsphere lens and the dynamic imaging methods using the system |
Non-Patent Citations (2)
| Title |
|---|
| 刘畅 等: "微球透镜超分辨成像研究进展与发展前景", 《激光与光电子学进展》 * |
| 王淑莹 等: "基于微球透镜的任选区高分辨光学显微成像新方法研究", 《物理学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111381355A (en) * | 2018-12-29 | 2020-07-07 | 南京培轩雅谱光电科技有限公司 | Optical imaging apparatus and method |
| CN112653834A (en) * | 2020-12-01 | 2021-04-13 | 广东鼎诚电子科技有限公司 | Super-resolution scanning imaging method, system and storage medium |
| CN112653834B (en) * | 2020-12-01 | 2022-04-08 | 广东鼎诚电子科技有限公司 | Super-resolution scanning imaging method, system and storage medium |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102735878B (en) | Super-resolution microscopic imaging method and system based on microcantilever and microsphere combined probe | |
| Chen et al. | Remote-mode microsphere nano-imaging: new boundaries for optical microscopes | |
| CN108152941B (en) | High speed optical super-resolution imaging system and method based on micro-nano lens array | |
| Pauzauskie et al. | Optical trapping and integration of semiconductor nanowire assemblies in water | |
| Korchev et al. | Scanning ion conductance microscopy of living cells | |
| CN107402443A (en) | A kind of optical ultra-discrimination rate imaging system based on inverted microscope and microsphere lens and the dynamic imaging methods using the system | |
| CN106198489B (en) | A kind of molecule knot optical near-field microscopic system and its building method | |
| CN108445262A (en) | A kind of scanning microsphere lens super-resolution microscope and its imaging method based near field optic | |
| CN105988021A (en) | Optical super-resolution dynamic imaging system and method based on microlens modified probe | |
| CN101430321B (en) | Non-contact biological detection scanning nano-glass probe microscope and its operation method | |
| CN102662086B (en) | Multiple-degree-of-freedom near-field optical microscope based on micro-nano motion arm | |
| CN105301290B (en) | A kind of phase-modulation imaging mode scan device and method of SICM | |
| JP2008519423A (en) | Integrated sub-nanometer scale electron beam system | |
| CN106772990B (en) | A kind of light control techniques for realizing the adjustment of cell tandem using double optical fiber optical tweezers | |
| CN102071135A (en) | High resolution patch clamp based on scanning probe microscopy technology and operating method thereof | |
| CN107727886B (en) | Inverted high-speed electrochemical atomic force microscope | |
| Lieberman et al. | Multifunctional, micropipette based force cantilevers for scanned probe microscopy | |
| CN101788571A (en) | Nanomanipulation method for compounding laser near-field optical tweezers and AFM probe | |
| CN108445261A (en) | A kind of microsphere lens super resolution optical scanning imaging system and its imaging method based on optical position sensitive sensors feedback | |
| CN106365111A (en) | Preparation method for Pt disk sub-micron electrode with controllable geometrical shape | |
| CN114815208A (en) | Super-resolution imaging system and imaging method based on micro-bottle lens | |
| CN208334801U (en) | A kind of optical ultra-discrimination rate imaging system based on optical tweezer and microsphere lens | |
| CN109001903A (en) | High-order mode optical tweezer and its test method based on four mode fiber of biconial | |
| CN204389528U (en) | The optical ultra-discrimination rate dynamic imaging system of probe is modified based on lenticule | |
| CN104777602B (en) | It is a kind of to bore optical tweezer classification with hollow optic fibre and collect the device of air PM2.5 particles |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180824 |
|
| RJ01 | Rejection of invention patent application after publication |