CN108434508A - A kind of rush wound healing gel dressing and preparation method thereof - Google Patents

A kind of rush wound healing gel dressing and preparation method thereof Download PDF

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Publication number
CN108434508A
CN108434508A CN201810603875.9A CN201810603875A CN108434508A CN 108434508 A CN108434508 A CN 108434508A CN 201810603875 A CN201810603875 A CN 201810603875A CN 108434508 A CN108434508 A CN 108434508A
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preparation
wound healing
silk screen
collagen
solution
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胡张艳
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
    • A61L15/28—Polysaccharides or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/20—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing organic materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
    • A61L15/26—Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
    • A61L15/32—Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin
    • A61L15/325—Collagen
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/42—Use of materials characterised by their function or physical properties
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/42—Use of materials characterised by their function or physical properties
    • A61L15/44—Medicaments
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/42—Use of materials characterised by their function or physical properties
    • A61L15/46—Deodorants or malodour counteractants, e.g. to inhibit the formation of ammonia or bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/42—Use of materials characterised by their function or physical properties
    • A61L15/62—Compostable, hydrosoluble or hydrodegradable materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/202—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials with halogen atoms, e.g. triclosan, povidone-iodine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/404—Biocides, antimicrobial agents, antiseptic agents

Abstract

The present invention provides a kind of rush wound healing gel dressings and preparation method thereof, the rush wound healing gel dressing is prepared by collagen solution and Static Spinning silk screen, and the collagen solution is made of collagen protein powder, the ethanol solution of acetic acid, vitamin E, 6 cyclohexyl, 1 hydroxyl 4 methyl 2 (1H) pyridone, N HOSu NHSs and povidone iodine；The Static Spinning silk screen is made of polylactide glycolic acid copolymer, hydroxypropyl chitosan, polycaprolactone, medical chitose, double methyl methacrylate triethylene glycol ester and 2 hydroxyl, 2 methylpropiophenone；Wound healing gel dressing biodegradability of the present invention is good, and has good physiological adaptation, and antibiotic property is strong, and the healing of wound and the growth of epithelial cell, adhesion inhibiting properties can be promoted strong.

Description

A kind of rush wound healing gel dressing and preparation method thereof
Technical field
The present invention relates to medical material tech fields, specifically, the present invention relates to a kind of rush wound healing gels Dressing and preparation method thereof.
Background technology
Operation refers to doctor and the treatments such as cuts off, sutures to patient body with medical instrument, with knife, cut, the instruments such as needle exist Body local is operated, and is the primary treatments of surgery, is commonly called as " operating on ".Operation is a kind of destruction tissue integrity（It cuts It opens）, or integrality is made to be restored by the tissue destroyed（Suture）Operation.The purpose of operation is to cure or diagnose the illness, and is such as gone Except pathological tissues, reparation damage, transplant organ, the function of improving body and form etc..And skin is human body and external environment Barrier, in protective various tissues and organ from extraneous physical, mechanicalness, chemically and the invasion of pathogenic microorganism. It after human body, which is damaged, is performed the operation, needs to wrap up wound, promotes wound healing, and wound surface needs Covering dressing in time, otherwise there is also wound infection, and wound is caused a large amount of diffusate occur, decompose extracellular matrix protein And a variety of growth factors, promoting epidermization and wound closure are hindered, to significantly affect the process of rehabilitation, it could even be possible to causing to suffer from Person is dead.
Medical dressing is a kind of medical material covered for various wounds, wound surface, wound can be made from thin Bacterium infects and the influence of other foeign elements, plays the role of protecting wound, the surface of a wound.Traditional dressing mainly utilizes bedding and padding, yarn Cloth, lining cloth or similar covering protect wound, the surface of a wound, and important function has been played in medicine and hygiene fields.
To prevent patient from sticking together after surgery, occurs the not similar shape such as solution-type, gel-type or film-type in recent years The dressing of the prevention of postoperative adhesion of formula, and some is applied in clinic, it is insoluble including water dissolvable and water Property, the various traits such as biodegradability and biological non-biodegradable, natural polymer subtype and the high parting of synthesis, unlike material and The dressing that prevents adhesion of different shape.Although these dressing also respectively have difference in form and character in different, effect, to anti- Only the generation of post-operation adhesion has certain effect, but these dressing are easily dry after imbibition, and newborn group is damaged when causing to remove It knits, brings great pain to patient, and liquid absorption amount is small, wrapping thickness is big, poor air permeability, and replacement frequency is high.Therefore, it develops Go out a kind of with, without tolerance, with good biocompatibility, degradability is high, can promote wound after the rapid, long-time service that works Mouth healing and safe dressing are the developing direction and trend in the field.
Invention content
It is an object of the invention to the deficiencies for existing dressing, solve defect in the prior art, to solve above-mentioned ask Topic heals gel dressing and preparation method thereof the present invention provides a kind of rushs wound, with degradability, and antibiotic property, The reparation of tissue physiology's property and preventing tissue adhesive are very strong.
Above-mentioned purpose that the invention is realized by the following technical scheme, a kind of rush wound healing gel dressing, the rush Wound healing gel dressing is prepared by collagen solution and Static Spinning silk screen, and the collagen solution is by collagen Albumen powder, the ethanol solution of acetic acid, vitamin E, -2 (1H)-pyridone of 6- cyclohexyl -1- hydroxy-4-methyls, N- hydroxysuccinimidyls Acid imide is formed with povidone iodine；The Static Spinning silk screen by polylactide glycolic acid copolymer, hydroxypropyl chitosan, gather oneself in Ester, medical chitose, double methyl methacrylate triethylene glycol ester and 2- hydroxy-2-methyls-phenylacetone composition.
A kind of preparation method promoting wound healing gel dressing, includes the following steps：
（1）, collagen solution preparation：
Collagen protein powder is dissolved in the ethanol solution of acetic acid, vitamin E, 6- cyclohexyl -1- hydroxy-4-methyls -2 are added (1H)-pyridone, n-hydroxysuccinimide and povidone iodine, are sufficiently stirred, and collagen solution is made；
（2）, Static Spinning silk screen preparation：
1. polylactide glycolic acid copolymer, hydroxypropyl chitosan, polycaprolactone and medical chitose are dissolved in tetrahydrofuran, N, In the mixed solution of dinethylformamide, agitating solution adds double methyl methacrylate triethylene glycol ester and 2- hydroxyls- Electrostatic spinning liquid is made in 2- methylphenyl acetone；
2. electrostatic spinning liquid obtained above is carried out electrostatic spinning on electrospinning device, then electrostatic spinning is woven into Net irradiates 20min under ultraviolet source irradiation, carries out crosslinked Static Spinning silk screen；
（3）, promote wound healing gel dressing preparation：
By step（2）The Static Spinning silk screen of middle preparation is placed in step（1）In the collagen solution of middle preparation, ultrasound leaching at room temperature Stain 2h takes out the gel silk screen with collagen after dipping, is lyophilized at -70 DEG C to get healing gel to promoting wound Dressing.
Further, the step（1）The ethanol solution concentration of middle acetic acid is 1mol/L, the n-hydroxysuccinimide A concentration of 0.002%wt, the vitamin E, 6- cyclohexyl -1- hydroxy-4-methyls -2 (1H)-pyridone and povidone iodine weight Amount is than being 1:2:2.
Further, the step（2）A concentration of 12-15wt% of middle polylactide glycolic acid copolymer, the hydroxypropyl A concentration of 3-5wt% of chitosan, a concentration of 5-8wt% of polycaprolactone, a concentration of 3-7wt% of medical chitose, double first A concentration of 3wt% of base acrylic acid Triethylene Glycol, a concentration of 2wt% of the 2- hydroxy-2-methyls-phenylacetone.
Further, the step（2）The volume ratio of middle tetrahydrofuran and N,N-dimethylformamide is 1:2.
Further, the step（2）Middle ultraviolet source luminous intensity is 210Mw/cm3。
Dressing of the present invention is prepared by collagen solution and Static Spinning silk screen, compared with prior art, is had following Advantageous effect：
（1）, collagen solution using collagen protein powder, the ethanol solution of acetic acid, vitamin E, 6- cyclohexyl -1- hydroxyls -4- Methyl -2 (1H)-pyridone, n-hydroxysuccinimide and povidone iodine form, and have antibacterial action, contribute to solution to quiet The wetting of electrospinning silk screen with penetrate, the healing of skin trauma mouth and the growth of epithelial cell can be promoted.
（2）, Static Spinning silk screen using polycaprolactone prepare, it is fine with biological cell compatibility in vivo and degradable At CO2And H2O, biodegradability is good, and has good physiological adaptation.
（3）, Static Spinning silk screen using medical chitose prepare, because medical chitose is the macromolecule chemical combination purified by crab shell Object chitin（chitin）, manufactured a kind of poly glucosamine after de- N-acetyl group again deep processing is a kind of with good The medical high polymer polysaccharose substance of biocompatibility, biodegradability and biological activity.Medical chitose has selection Property promote epithelial cell, endothelial cell growth and inhibit the biological nature of fibroblastic growth, to promote tissue physiology's property It repairs, scar is inhibited to be formed, reduce tissue adhesion.
（4）, Static Spinning silk screen using hydroxypropyl chitosan prepare, hydroxypropyl chitosan is one made of chitin modified The water-soluble good chitosan derivatives of kind, it can be dissolved in water but also be dissolved in the organic solvents such as ethyl alcohol, have good emulsification Property, antibiotic property, moisture absorbability and moisture retentivity and surface-active, moisture-absorbing moisture-keeping ability and hyaluronic acid（HA）Quite, emulsibility and foam Property is suitable with nonionic surfactant Tween60.
Description of the drawings
Fig. 1 is rush wound healing gel dressing degradation rate figure in phosphate buffer solution prepared by embodiment 2.
Fig. 2 is the electron-microscope scanning figure of rush wound healing gel dressing prepared by embodiment 3.
Specific implementation mode
Present invention is further elaborated in following combination specific embodiment, and embodiment only helps understand the present invention, but not Present disclosure can be limited, any restrictions of the scope of the invention are should not be construed as.
Embodiment 1
（1）, collagen solution preparation：
By collagen protein powder（Ox heel string collagen derives from Sigma-Aldrich (Shanghai) trade Co., Ltd）It is dissolved in The ethanol solution of 1mol/L acetic acid（100ml）In, add vitamin E（1.8g）, 6- cyclohexyl -1- hydroxy-4-methyls -2 (1H)-pyridone（3.6g）、0.002%wt（Based on mixed solution weight）N-hydroxysuccinimide and povidone iodine （3.6g）, it is sufficiently stirred, collagen solution is made；
（2）, Static Spinning silk screen preparation：
1. by 12wt%（Based on mixed solution weight）Polylactide glycolic acid copolymer, 3wt%（Mixed solution weight is base Plinth）Hydroxypropyl chitosan, 5wt%（Based on mixed solution weight）Polycaprolactone and 3wt%（Mixed solution weight is base Plinth）Medical chitose be dissolved in tetrahydrofuran（30ml）, N,N-dimethylformamide（60ml）Mixed solution in, stirring it is molten Liquid adds 3wt%（Based on mixed solution weight）Double methyl methacrylate triethylene glycol ester and 3wt%（Mixed solution Based on weight）2- hydroxy-2-methyls-phenylacetone be made electrostatic spinning liquid；
2. electrostatic spinning liquid obtained above is carried out electrostatic spinning on electrospinning device, then electrostatic spinning is woven into Net is 210Mw/cm in luminous intensity320min is irradiated under ultraviolet source irradiation, carries out crosslinked Static Spinning silk screen；
（3）, promote wound healing gel dressing preparation：
By step（2）The Static Spinning silk screen of middle preparation is placed in step（1）In the collagen solution of middle preparation, ultrasound leaching at room temperature Stain 2h takes out the gel silk screen with collagen after dipping, is lyophilized at -70 DEG C to get healing gel to promoting wound Dressing.
Porosity measurement is carried out to rush wound healing gel dressing prepared by embodiment 1：
The rush wound healing gel dressing that Example 1 is prepared is cut into the sample of 2mm*2mm, in the centrifuge tube of 10 mL In, it fills absolute ethyl alcohol and weighs M1, appropriate amount of sample M2 is taken to immerse in absolute ethyl alcohol, the gas in ultrasonic vibration to complete removing sample Bubble, makes ethyl alcohol fill in sample structure completely, and absolute ethyl alcohol is added into centrifuge tube to being full of, is weighed as M3.Taking-up is filled nothing The sample of water-ethanol, it is M4 to weigh remaining quality, and calculating porosity is θ, is repeated 5 times measurement, seeks the average value of porosity.
The porosity of rush wound healing gel dressing prepared by embodiment 1 is 94.6%, and porosity height can improve more Big space is used for absorbing the body fluid dissolved out at postoperative wound, while adsorbing and activating intra platelet free calcium coagulation factor, to wound Hemostasis, prevents tissue adhesion.
Embodiment 2
（1）, collagen solution preparation：
By collagen protein powder（Ox heel string collagen derives from Sigma-Aldrich (Shanghai) trade Co., Ltd）It is dissolved in The ethanol solution of 1mol/L acetic acid（120ml）In, add vitamin E（2.2g）, 6- cyclohexyl -1- hydroxy-4-methyls -2 (1H)-pyridone（4.4g）、0.002%wt（Based on mixed solution weight）N-hydroxysuccinimide and povidone iodine （4.4g）, it is sufficiently stirred, collagen solution is made；
（2）, Static Spinning silk screen preparation：
1. by 14wt%（Based on mixed solution weight）Polylactide glycolic acid copolymer, 5wt%（Mixed solution weight is base Plinth）Hydroxypropyl chitosan, 7wt%（Based on mixed solution weight）Polycaprolactone and 3wt%（Mixed solution weight is base Plinth）Medical chitose be dissolved in tetrahydrofuran（40ml）, N,N-dimethylformamide（80ml）Mixed solution in, stirring it is molten Liquid adds 3wt%（Based on mixed solution weight）Double methyl methacrylate triethylene glycol ester and 2wt%（Mixed solution Based on weight）2- hydroxy-2-methyls-phenylacetone be made electrostatic spinning liquid；
2. electrostatic spinning liquid obtained above is carried out electrostatic spinning on electrospinning device, then electrostatic spinning is woven into Net is 210Mw/cm in luminous intensity320min is irradiated under ultraviolet source irradiation, carries out crosslinked Static Spinning silk screen；
（3）, promote wound healing gel dressing preparation：
By step（2）The Static Spinning silk screen of middle preparation is placed in step（1）In the collagen solution of middle preparation, ultrasound leaching at room temperature Stain 2h takes out the gel silk screen with collagen after dipping, is lyophilized at -70 DEG C to get healing gel to promoting wound Dressing.
Degradation rate test is carried out to rush wound healing gel dressing prepared by embodiment 2：
Example 2 prepare rushs wound healing gel dressing quality for W1 be submerged into phosphate buffer solution (PH= 7.4) it in, is positioned in incubator（37 DEG C of cultures）, take out respectively at 14 days, 28 days, 42 days, 56 days, 70 days, weigh after dry W2, meanwhile, PBS is replaced, material is submerged into liquid after weighing, is positioned in incubator again, until next testing time point, is pressed Mass loss rate is calculated according to following formula（γ）.
Rush wound healing gel prepared by embodiment 2 is observed after the 14th day, the 28th day, the 42nd day, the 56th day to apply Expect the degradation rate in phosphate buffer solution, the results are shown in Figure 1.
As shown in Figure 1, the rush wound healing gel dressing that prepared by embodiment 2 is degraded speed in phosphate buffer solution Rate is substantially linear constant.
Embodiment 3
（1）, collagen solution preparation：
By collagen protein powder（Ox heel string collagen derives from Sigma-Aldrich (Shanghai) trade Co., Ltd）It is dissolved in The ethanol solution of 1mol/L acetic acid（120ml）In, add vitamin E（2.2g）, 6- cyclohexyl -1- hydroxy-4-methyls -2 (1H)-pyridone（4.4g）、0.002%wt（Based on mixed solution weight）N-hydroxysuccinimide and povidone iodine （4.4g）, it is sufficiently stirred, collagen solution is made；
（2）, Static Spinning silk screen preparation：
1. by 12wt%（Based on mixed solution weight）Polylactide glycolic acid copolymer, 5wt%（Mixed solution weight is base Plinth）Hydroxypropyl chitosan, 5.2wt%（Based on mixed solution weight）Polycaprolactone and 4.1wt%（Mixed solution weight is Basis）Medical chitose be dissolved in tetrahydrofuran（40ml）, N,N-dimethylformamide（80ml）Mixed solution in, stirring it is molten Liquid adds 3wt%（Based on mixed solution weight）Double methyl methacrylate triethylene glycol ester and 2wt%（Mixed solution Based on weight）2- hydroxy-2-methyls-phenylacetone be made electrostatic spinning liquid；
2. electrostatic spinning liquid obtained above is carried out electrostatic spinning on electrospinning device, then electrostatic spinning is woven into Net is 210Mw/cm in luminous intensity320min is irradiated under ultraviolet source irradiation, carries out crosslinked Static Spinning silk screen；
（3）, promote wound healing gel dressing preparation：
By step（2）The Static Spinning silk screen of middle preparation is placed in step（1）In the collagen solution of middle preparation, ultrasound leaching at room temperature Stain 2h takes out the gel silk screen with collagen after dipping, is lyophilized at -70 DEG C to get healing gel to promoting wound Dressing.
Electron-microscope scanning is carried out to the rush wound healing gel dressing that embodiment 3 is prepared, the results are shown in Figure 2.

Claims (6)

  1. The gel dressing 1. a kind of rush wound heals, which is characterized in that the rush wound heals gel dressing by collagen Protein solution is prepared with Static Spinning silk screen, and the collagen solution is given birth to by collagen protein powder, the ethanol solution of acetic acid, dimension Plain -2 (1H)-pyridone of E, 6- cyclohexyl -1- hydroxy-4-methyls, n-hydroxysuccinimide and povidone iodine form；It is described quiet Electrospinning silk screen is by polylactide glycolic acid copolymer, hydroxypropyl chitosan, polycaprolactone, medical chitose, methacrylate Triethylene Glycol is formed with 2- hydroxy-2-methyls-phenylacetone.
  2. 2. a kind of preparation method promoting wound healing gel dressing, which is characterized in that include the following steps：
    （1）, collagen solution preparation：
    Collagen protein powder is dissolved in the ethanol solution of acetic acid, vitamin E, 6- cyclohexyl -1- hydroxy-4-methyls -2 are added (1H)-pyridone, n-hydroxysuccinimide and povidone iodine, are sufficiently stirred, and collagen solution is made；
    （2）, Static Spinning silk screen preparation：
    1. polylactide glycolic acid copolymer, hydroxypropyl chitosan, polycaprolactone and medical chitose are dissolved in tetrahydrofuran, N, In the mixed solution of dinethylformamide, agitating solution adds double methyl methacrylate triethylene glycol ester and 2- hydroxyls- Electrostatic spinning liquid is made in 2- methylphenyl acetone；
    2. electrostatic spinning liquid obtained above is carried out electrostatic spinning on electrospinning device, then electrostatic spinning is woven into Net irradiates 20min under ultraviolet source irradiation, carries out crosslinked Static Spinning silk screen；
    （3）, promote wound healing gel dressing preparation：
    By step（2）The Static Spinning silk screen of middle preparation is placed in step（1）In the collagen solution of middle preparation, ultrasound leaching at room temperature Stain 2h takes out the gel silk screen with collagen after dipping, is lyophilized at -70 DEG C to get healing gel to promoting wound Dressing.
  3. 3. a kind of preparation method promoting wound healing gel dressing according to claim 2, which is characterized in that described Step（1）The ethanol solution concentration of middle acetic acid is 1mol/L, a concentration of 0.002%wt of n-hydroxysuccinimide, the dimension The weight ratio of raw -2 (1H)-pyridone of element E, 6- cyclohexyl -1- hydroxy-4-methyls and povidone iodine is 1:2:2.
  4. 4. a kind of preparation method promoting wound healing gel dressing according to claim 2, which is characterized in that described Step（2）A concentration of 12-15wt% of middle polylactide glycolic acid copolymer, a concentration of 3-5wt% of the hydroxypropyl chitosan, A concentration of 5-8wt% of polycaprolactone, a concentration of 3-7wt% of medical chitose, the methacrylate triethylene-glycol A concentration of 3wt% of ester, a concentration of 2wt% of the 2- hydroxy-2-methyls-phenylacetone.
  5. 5. a kind of preparation method promoting wound healing gel dressing according to claim 2, which is characterized in that described Step（2）The volume ratio of middle tetrahydrofuran and N,N-dimethylformamide is 1:2.
  6. 6. a kind of preparation method promoting wound healing gel dressing according to claim 2, which is characterized in that described Step（2）Middle ultraviolet source luminous intensity is 210Mw/cm3。
CN201810603875.9A 2018-06-12 2018-06-12 A kind of rush wound healing gel dressing and preparation method thereof Withdrawn CN108434508A (en)

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CN110318160A (en) * 2019-07-23 2019-10-11 大连海关技术中心 A kind of preparation method and application of chitosan derivatives nano fibrous membrane wound dressing
CN115192763A (en) * 2022-07-08 2022-10-18 重庆科技学院 Multifunctional temperature-regulating wound dressing and preparation method and application thereof

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109589447A (en) * 2018-11-13 2019-04-09 王岩松 A kind of preparation method and applications of medical injectable antibacterial composite in-situ hydrogel
CN110318160A (en) * 2019-07-23 2019-10-11 大连海关技术中心 A kind of preparation method and application of chitosan derivatives nano fibrous membrane wound dressing
CN110318160B (en) * 2019-07-23 2021-11-09 大连海关技术中心 Preparation method and application of chitosan derivative nanofiber membrane wound dressing
CN115192763A (en) * 2022-07-08 2022-10-18 重庆科技学院 Multifunctional temperature-regulating wound dressing and preparation method and application thereof
CN115192763B (en) * 2022-07-08 2023-12-12 重庆科技学院 Multifunctional temperature-regulating wound dressing and preparation method and application thereof

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