CN108414752A - A kind of bacterium automatic sorting labeling method and device based on immunization method - Google Patents
A kind of bacterium automatic sorting labeling method and device based on immunization method Download PDFInfo
- Publication number
- CN108414752A CN108414752A CN201810266026.9A CN201810266026A CN108414752A CN 108414752 A CN108414752 A CN 108414752A CN 201810266026 A CN201810266026 A CN 201810266026A CN 108414752 A CN108414752 A CN 108414752A
- Authority
- CN
- China
- Prior art keywords
- bacterium
- antibody molecule
- nanogold
- tank body
- control
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Food Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention discloses a kind of bacterium automatic sorting labeling method and device based on immunization method, including tank body, footing, outlet tube and agitating roller, the tank body bottom surface periphery is provided with the footing, the footing side is provided with the outlet tube, the outlet tube end set has end cap, the tank body top surface is provided with electric machine stand, and servo motor is provided on the electric machine stand.Advantageous effect is：Using flow sensor, control host and solenoid valve block at feedback control loop, the input quantity of storage bin interior antibody molecule can be automatically controlled to preset value, agitating roller rotation is driven to be stirred analyte sample fluid and antibody molecule by servo motor simultaneously, improve the working efficiency of bacterium sorting indicia, and the video information and real-time display on a display screen of bacterium sorting indicia working condition can be obtained in real time by infrared camera, convenient for users to accurately holding the job schedule of bacterium sorting indicia, be conducive to promote on a large scale.
Description
Technical field
The present invention relates to biological sample sorting technology fields, and in particular to a kind of bacterium automatic sorting based on immunization method
Labeling method and device.
Background technology
With the continuous development of life science, biology of bacteria and amynologic basis research and clinical disease diagnosis with
Treatment is required for separation and purification of bacterial, existing bacterium separation technology from the mixing sample containing different bacterium mainly to have
Ficoll density gradient centrifugations, centrifugation elution, aqueous two-phase separation etc., these methods are all to utilize bacterium size, density, surface electricity
The feature of the physical properties such as lotus is detached, it is difficult to obtain ideal purity and the rate of recovery, the cell sorting based on magnetic bead
It is the cell separation technology developed rapidly in recent years, marks antibody molecule on magnetic bead first, makes itself and cell hair
Raw specific binding, then by the effect of external magnetic field, make the cell combined with magnetic bead generation displacement and realize aim cell
Separation, the method is easy to operate, rapid, extensive instrument is not needed, and can obtain higher purity and the rate of recovery, to biological sample
This analysis often needs to carry out sorting indicia to bacterium often, and the Chinese patent of 102243234 A of Publication No. CN discloses one
Bacterium automatic sorting labeling method of the kind based on immunization method, includes the following steps：1) magnetic bead coated antibody molecule；2) fluorescence
Matter coated antibody molecule；3) sample liquid is filled；4) combination of immunomagnetic beads and purpose bacterium；5) Magneto separate；6) it cleans；7) fluorescence
Mass signatures purpose bacterium, above-mentioned bacterium automatic sorting labeling method and device based on immunization method can not be according to samples to be tested
The amount of liquid carrys out the input quantity of automatic quantitative antibody processed molecule, while being not provided with agitating function, the work of bacterium sorting indicia
Efficiency is general, and can not obtain the job schedule of bacterium sorting indicia in real time, and then can not accurately hold bacterium sorting indicia
Job schedule.
Invention content
The purpose of the present invention is that provide a kind of bacterium based on immunization method to solve the above-mentioned problems divides automatically
Select labeling method and device.
The present invention is achieved through the following technical solutions above-mentioned purpose：
According to an aspect of the present invention, a kind of bacterium automatic sorting labelling apparatus based on immunization method is provided, including tank body,
Footing, outlet tube and agitating roller, the tank body bottom surface periphery are provided with the footing, the footing side be provided with it is described go out liquid
Pipe, the outlet tube end set have end cap, the tank body top surface to be provided with electric machine stand, be provided with and watch on the electric machine stand
Take motor, the servo motor end set has a shaft, and the roller end is provided with the agitating roller, by the electric machine stand
Side is provided with inlet tube, and another side of electric machine stand is provided with storage bin, and the storage bin top surface is provided with feed hopper, institute
It states storage bin bottom surface and is provided with discharge bucket, outside is provided with solenoid valve in the middle part of the discharge bucket, and the discharge bucket inner side is set
It is equipped with flow sensor, the tank body side is provided with side door, and sealing strip is provided with around the side door, and the side door surface is set
It is equipped with handle, the tank interior top surface is provided with infrared camera, and the tank interior side is provided with insulation board, described exhausted
Listrium side is provided with side plate, and the side plate bottom side is provided with limited block, and the side plate top surface is provided with electrostatic generator,
It is provided with control host before the tank body, display screen is provided on the control host, the display screen side is provided with control
Keyboard processed.
In the present embodiment, the quantity of the footing is four, and the footing is solid construction, and the outlet tube passes through spiral shell
Line is fixed on the tank body, and the end cap coordinates with the outlet tube transition.
In the present embodiment, the material of the electric machine stand is cast iron, and the rated speed of the servo motor is not less than
The class of insulation of 720Rpm, the servo motor are F grades, and the cross sectional shape of the shaft is one in round or octagon
Kind, the shaft is solid construction, and the shaft is connect with the servo motor by shaft coupling.
In the present embodiment, the material of the agitating roller is aluminium alloy, and the agitating roller is connected with the shaft by clip
It connects, the radial dimension of the inlet tube is that the radial dimension of the outlet tube is twice, and the quantity of the storage bin is two, institute
The volume for stating storage bin is not less than 5L.
In the present embodiment, the feed hopper is connected through a screw thread with the storage bin, the radial dimension of the discharge bucket
Identical as the radial dimension of the feed hopper, the action response time of the solenoid valve is no more than 0.5s, and the solenoid valve passes through
Screw element is fixed on the discharge bucket.
In the present embodiment, the work refreshing frequency of the flow sensor is not less than 60HZ, the side door and the tank
Body is by hinge connection, and the material of the sealing strip is quenched rubber, and the thickness of the sealing strip is not less than 4mm.
In the present embodiment, the material of the handle is phenolic resin, and the resolution ratio of the infrared camera is not less than
The material of 720P, the insulation board are silicon carbide, and the thickness of the insulation board is not less than 5mm.
In the present embodiment, the material of the side plate is stainless steel, and the side plate is sieve texture, the sieve pore of the side plate
Mesh number is not less than 800, and the quantity of the limited block is not less than four, exists in pairs, the peak value of pulse electricity of the electrostatic generator
Pressure is not more than 120Kv.
In the present embodiment, the material of the model PLC S7-1200 of the control host, the display screen are IPS, institute
It states control keyboard to be embedded on the control host, the control host and the display screen, the servo motor, the electromagnetism
Valve, the electrostatic generator, the flow sensor, the infrared camera and control keyboard electrical connection.
The above-mentioned bacterium automatic sorting labeling method based on immunization method, includes the following steps：
（1）Nanogold is combined with antibody molecule, obtains the nanogold of antibody molecule, the antibody molecule energy in nanogold and mesh
Bacterium specific binding, while fluorescent material being combined with antibody molecule, obtains the fluorescent material of antibody molecule, fluorescence
Antibody molecule in matter can be specifically bound with purpose bacterium, and fluorescent material is that isosulfocyanic acid fluorescence is orange red, isosulfocyanic acid fluorescence
Huang, isosulfocyanic acid fluorescence is red, fluorescein isothiocynate isomers I, one kind in 5-isothiocyanate fluorescein；
（2）Inspection ensure it is described capping be fastened in the outlet tube, while it is ensured that the side door be in close sealing state,
The nanogold for having antibody molecule and there will be the fluorescent material of antibody molecule to be separately added into two storage bins later, simultaneously will
Analyte sample fluid is added to through the inlet tube in the tank body；
（3）The amount for the nanogold that antibody molecule need to be added is confirmed according to the amount of analyte sample fluid, passes through the control later
Solenoid valve described in Keyboard Control is opened, and has the nanogold of antibody molecule to enter in the tank body through the discharge bucket, while described
Flow sensor work obtains the flow information at the discharge bucket and is back to the control host, the control host in real time
Flow information is converted to and is compared with the nanogold amount for being preset with antibody molecule after real-time load, after real-time load
When identical as the nanogold amount of antibody molecule is preset with, the solenoid valve is automatically closed；
（4）Controlling the servo motor work by the control keyboard drives the shaft to rotate, described in the shaft drive
Agitating roller rotation is by analyte sample fluid and has the nanogold of antibody molecule to stir combination, the antibody molecule energy and mesh of nanometer gold surface
Bacterial surface antigen specific binding, to obtain the mixture of nanogold and purpose bacterium；
（5）The electrostatic generator work is controlled by the control keyboard and generates electrostatic, and electrostatic uniformly divides on the side plate
Cloth, nanogold and the mixture of purpose bacterium are attached to after Electrostatic Absorption on the side plate, are made by agitating roller rotation
The mixture of nanogold and purpose bacterium stops after being substantially adhered on the side plate；
（6）The amount for the fluorescent material that antibody molecule need to be added is confirmed according to the amount of analyte sample fluid, passes through the control later
Solenoid valve described in Keyboard Control processed is opened, and has the fluorescent material of antibody molecule to enter in the tank body through the discharge bucket, simultaneously
The flow sensor work obtains the flow information at the discharge bucket and is back to the control host, the control in real time
Flow information is converted to and is compared with the fluorescence quality for being preset with antibody molecule after real-time load by host, is gone out when in real time
After doses be preset with antibody molecule fluorescence it is identical in quality when, the solenoid valve is automatically closed；
（7）The electrostatic generator is closed, and controls the agitating roller rotation to make to have the fluorescent material and nanometer of antibody molecule
Gold is fully combined with the mixture of purpose bacterium, and the antibody molecule of phosphor surface can be with the mixing of nanogold and purpose bacterium
Object surface antigen is specifically bound, and to obtain the mixture of fluorescent material and nanogold and purpose bacterium, and then is completed to mesh
Bacterium sorting indicia；
（8）Fluorescent material and nanogold and the mixture of purpose bacterium are existed by Electrostatic Absorption after starting the electrostatic generator
On the side plate, controls the infrared camera work and obtain the video information of bacterium sorting indicia working condition in real time and aobvious
Real-time display in display screen determines the job schedule of bacterium sorting indicia by observing the display screen, after the completion of sorting indicia,
It opens the end cap analyte sample fluid is discharged through the outlet tube, and opens the side door and take out the side plate to purpose
Bacterium carries out subsequent processing.
Advantageous effect is：Using flow sensor, control host and solenoid valve block at feedback control loop, can from
The input quantity of dynamic control storage bin interior antibody molecule drives agitating roller rotation come to be measured to preset value, while by servo motor
Sample liquid and antibody molecule are stirred, and improve the working efficiency of bacterium sorting indicia, and can be real by infrared camera
When obtain the video information and real-time display on a display screen of bacterium sorting indicia working condition, convenient for users to accurately holding carefully
The job schedule of bacterium sorting indicia is conducive to promote on a large scale.
Description of the drawings
Fig. 1 is a kind of positive exterior view of bacterium automatic sorting labelling apparatus based on immunization method of the present invention；
Fig. 2 is that a kind of bacterium automatic sorting labelling apparatus based on immunization method of the present invention faces interior view；
Fig. 3 is a kind of left view of bacterium automatic sorting labelling apparatus based on immunization method of the present invention；
Fig. 4 is a kind of vertical view of bacterium automatic sorting labelling apparatus based on immunization method of the present invention.
1, servo motor；2, electric machine stand；3, feed hopper；4, storage bin；5, tank body；6, display screen；7, footing；8, it feeds
Pipe；9, shaft；10, host is controlled；11, control keyboard；12, outlet tube；13, it covers；14, discharge bucket；15, solenoid valve；16, quiet
Electric generator；17, side plate；18, insulation board；19, flow sensor；20, infrared camera；21, agitating roller；22, limited block；
23, sealing strip；24, side door；25, handle.
Specific implementation mode
The invention will be further described below in conjunction with the accompanying drawings：
As Figure 1-Figure 4, according to an aspect of the present invention, a kind of bacterium automatic sorting label based on immunization method is provided
Device, including tank body 5, footing 7, outlet tube 12 and agitating roller 21,5 bottom surface periphery of the tank body is provided with the footing 7, described
7 side of footing is provided with the outlet tube 12, and 12 end set of the outlet tube has end cap, 5 top surface of the tank body to be provided with electricity
Machine engine base 2 is provided with servo motor 1 on the electric machine stand 2, and 1 end set of the servo motor has shaft 9, the shaft 9
End set has the agitating roller 21,2 side of the electric machine stand to be provided with inlet tube, and 2 another side of the electric machine stand is set
Be equipped with storage bin 4,4 top surface of the storage bin is provided with feed hopper 3, and 4 bottom surface of the storage bin is provided with discharge bucket 14, it is described go out
14 middle part outside of hopper is provided with solenoid valve 15, and 14 inner side of the discharge bucket is provided with flow sensor 19, the tank body 5
Side is provided with side door 24, sealing strip 23 is provided with around the side door 24,24 surface of the side door is provided with handle 25, described
5 inside top surface of tank body is provided with infrared camera 20, and 5 inner side of the tank body is provided with insulation board 18, the insulation board 18
Side is provided with side plate 17, and 17 bottom sides of the side plate are provided with limited block 22, and 17 top surface of the side plate is provided with electrostatic hair
Raw device 16,5 front of the tank body are provided with control host 10, display screen 6, the display screen are provided on the control host 10
6 sides are provided with control keyboard 11.
In the present embodiment, the quantity of the footing 7 is four, and the footing 7 is solid construction, and the outlet tube 12 is logical
It crosses and is threadedly secured on the tank body 5, the end cap coordinates with 12 transition of the outlet tube.
In the present embodiment, the material of the electric machine stand 2 is cast iron, and the rated speed of the servo motor 1 is not less than
The class of insulation of 720Rpm, the servo motor 1 are F grades, and the cross sectional shape of the shaft 9 is one in round or octagon
Kind, the shaft 9 is solid construction, and the shaft 9 is connect with the servo motor 1 by shaft coupling.
In the present embodiment, the material of the agitating roller 21 is aluminium alloy, and the agitating roller 21 passes through card with the shaft 9
Hoop connection, the radial dimension of the inlet tube are that the radial dimension of the outlet tube 12 is twice, and the quantity of the storage bin 4 is
Two, the volume of the storage bin 4 is not less than 5L.
In the present embodiment, the feed hopper 3 is connected through a screw thread with the storage bin 4, the radial direction of the discharge bucket 14
Size is identical as the radial dimension of the feed hopper 3, and the action response time of the solenoid valve 15 is no more than 0.5s, the electromagnetism
Valve 15 is fixed on by screw element on the discharge bucket 14.
In the present embodiment, the work refreshing frequency of the flow sensor 19 is not less than 60HZ, the side door 24 and institute
Tank body 5 is stated by hinge connection, the material of the sealing strip 23 is quenched rubber, and the thickness of the sealing strip 23 is not less than 4mm.
In the present embodiment, the material of the handle 25 is phenolic resin, and the resolution ratio of the infrared camera 20 is not low
It is silicon carbide in the material of 720P, the insulation board 18, the thickness of the insulation board 18 is not less than 5mm.
In the present embodiment, the material of the side plate 17 is stainless steel, and the side plate 17 is sieve texture, the side plate 17
Screen number be not less than 800, the quantity of the limited block 22 is not less than four, exists in pairs, the electrostatic generator 16
Peak impulse voltage is not more than 120Kv.
In the present embodiment, the model PLC S7-1200 of the control host 10, the material of the display screen 6 are
IPS, the control keyboard 11 are embedded on the control host 10, the control host 10 and the display screen 6, the servo
Motor 1, the solenoid valve 15, the electrostatic generator 16, the flow sensor 19, the infrared camera 20 and the control
Keyboard 11 processed is electrically connected.
The above-mentioned bacterium automatic sorting labeling method based on immunization method, includes the following steps：
（1）Nanogold is combined with antibody molecule, obtains the nanogold of antibody molecule, the antibody molecule energy in nanogold and mesh
Bacterium specific binding, while fluorescent material being combined with antibody molecule, obtains the fluorescent material of antibody molecule, fluorescence
Antibody molecule in matter can be specifically bound with purpose bacterium, and fluorescent material is that isosulfocyanic acid fluorescence is orange red, isosulfocyanic acid fluorescence
Huang, isosulfocyanic acid fluorescence is red, fluorescein isothiocynate isomers I, one kind in 5-isothiocyanate fluorescein；
（2）Inspection ensures that the capping 13 is fastened in the outlet tube 12, while it is ensured that the side door 24 is close in closing
Envelope state the nanogold for having antibody molecule and will have the fluorescent material of antibody molecule to be separately added into two storage bins 4 later
It is interior, while analyte sample fluid being added to through the inlet tube in the tank body 5；
（3）The amount for the nanogold that antibody molecule need to be added is confirmed according to the amount of analyte sample fluid, passes through the control later
Keyboard 11 controls the opening of the solenoid valve 15, has the nanogold of antibody molecule to enter in the tank body 5 through the discharge bucket 14, together
Shi Suoshu flow sensors 19, which work, the flow information obtained in real time at the discharge bucket 14 and is back to the control host 10,
Flow information is converted to and is compared with the nanogold amount for being preset with antibody molecule after real-time load by the control host 10,
When identical as the nanogold amount of antibody molecule is preset with after real-time load, the solenoid valve 15 is automatically closed；
（4）Controlling the work of the servo motor 1 by the control keyboard 11 drives the shaft 9 to rotate, and the shaft 9 drives
The rotation of the agitating roller 21 is by analyte sample fluid and has the nanogold of antibody molecule to stir combination, the antibody molecule of nanometer gold surface
It can be specifically bound with purpose bacterial surface antigen, to obtain the mixture of nanogold and purpose bacterium；
（5）The work of the electrostatic generator 16 is controlled by the control keyboard 11 and generates electrostatic, and electrostatic is on the side plate 17
It is uniformly distributed, nanogold and the mixture of purpose bacterium are attached to after Electrostatic Absorption on the side plate 17, pass through the stirring
Roller 21, which rotates after so that the mixture of nanogold and purpose bacterium is substantially adhered on the side plate 17, to be stopped；
（6）The amount for the fluorescent material that antibody molecule need to be added is confirmed according to the amount of analyte sample fluid, passes through the control later
Keyboard 11 processed controls the opening of the solenoid valve 15, has the fluorescent material of antibody molecule to enter the tank body 5 through the discharge bucket 14
It is interior, while the flow sensor 19 works and the flow information obtained in real time at the discharge bucket 14 and is back to control master
Machine 10, the control host 10 by flow information be converted to after real-time load be preset with the fluorescence quality of antibody molecule into
Row comparison, after real-time load be preset with antibody molecule fluorescence it is identical in quality when, the solenoid valve 15 is automatically closed；
（7）Close the electrostatic generator 16, and control the rotation of the agitating roller 21 come make to have the fluorescent material of antibody molecule with
Nanogold is fully combined with the mixture of purpose bacterium, and the antibody molecule of phosphor surface can be with nanogold and purpose bacterium
Mixture surface antigen is specifically bound, and to obtain the mixture of fluorescent material and nanogold and purpose bacterium, and then is completed
To the sorting indicia of purpose bacterium；
（8）Start and fluorescent material and nanogold and the mixture of purpose bacterium are passed through into Electrostatic Absorption after the electrostatic generator 16
On the side plate 17, control the infrared camera 20 work in real time obtain bacterium sorting indicia working condition video information
And the real-time display on display screen 6, determine the job schedule of bacterium sorting indicia, sorting mark by observing the display screen 6
After the completion of note, opens the end cap and analyte sample fluid is discharged through the outlet tube 12, and open the side door 24 by the side
Plate 17 takes out carries out subsequent processing to purpose bacterium.
The basic principles, main features and advantages of the invention have been shown and described above.The technical staff of the industry should
Understand, the present invention is not limited to the above embodiments, and the above embodiments and description only describe the originals of the present invention
Reason, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes and improvements
It all fall within the protetion scope of the claimed invention.
Claims (10)
1. a kind of bacterium automatic sorting labelling apparatus based on immunization method, it is characterised in that：Including tank body（5）, footing（7）、
Outlet tube（12）And agitating roller（21）, the tank body（5）Bottom surface periphery is provided with the footing（7）, the footing（7）Side is set
It is equipped with the outlet tube（12）, the outlet tube（12）End set has end cap, the tank body（5）Top surface is provided with electric machine stand
（2）, the electric machine stand（2）On be provided with servo motor（1）, the servo motor（1）End set has shaft（9）, described
Shaft（9）End set has the agitating roller（21）, the electric machine stand（2）Side is provided with inlet tube, the electric machine stand
（2）Another side is provided with storage bin（4）, the storage bin（4）Top surface is provided with feed hopper（3）, the storage bin（4）Bottom surface
It is provided with discharge bucket（14）, the discharge bucket（14）It is provided with solenoid valve on the outside of middle part（15）, the discharge bucket（14）Private side
Face is provided with flow sensor（19）, the tank body（5）Side is provided with side door（24）, the side door（24）Surrounding is provided with close
Strip of paper used for sealing（23）, the side door（24）Surface is provided with handle（25）, the tank body（5）Inside top surface is provided with infrared camera
（20）, the tank body（5）Inner side is provided with insulation board（18）, the insulation board（18）Side is provided with side plate（17）, institute
State side plate（17）Bottom sides are provided with limited block（22）, the side plate（17）Top surface is provided with electrostatic generator（16）, described
Tank body（5）Front is provided with control host（10）, the control host（10）On be provided with display screen（6）, the display screen（6）
Side is provided with control keyboard（11）.
2. a kind of bacterium automatic sorting labelling apparatus based on immunization method according to claim 1, it is characterised in that：Institute
State footing（7）Quantity be four, the footing（7）For solid construction, the outlet tube（12）It is threadedly secured to described
Tank body（5）On, the end cap and the outlet tube（12）Transition coordinates.
3. a kind of bacterium automatic sorting labelling apparatus based on immunization method according to claim 1, it is characterised in that：Institute
State electric machine stand（2）Material be cast iron, the servo motor（1）Rated speed be not less than 720Rpm, the servo motor
（1）The class of insulation be F grades, the shaft（9）Cross sectional shape be one kind in round or octagon, the shaft（9）
For solid construction, the shaft（9）With the servo motor（1）It is connected by shaft coupling.
4. a kind of bacterium automatic sorting labelling apparatus based on immunization method according to claim 1, it is characterised in that；Institute
State agitating roller（21）Material be aluminium alloy, the agitating roller（21）With the shaft（9）By clamp connection, the inlet tube
Radial dimension be the outlet tube（12）Radial dimension be twice, the storage bin（4）Quantity be two, the storing
Storehouse（4）Volume be not less than 5L.
5. a kind of bacterium automatic sorting labelling apparatus based on immunization method according to claim 1, it is characterised in that：Institute
State feed hopper（3）With the storage bin（4）It is connected through a screw thread, the discharge bucket（14）Radial dimension and the feed hopper
（3）Radial dimension it is identical, the solenoid valve（15）Action response time be no more than 0.5s, the solenoid valve（15）Pass through spiral shell
Line part is fixed on the discharge bucket（14）On.
6. a kind of bacterium automatic sorting labelling apparatus based on immunization method according to claim 1, it is characterised in that：Institute
State flow sensor（19）Work refreshing frequency be not less than 60HZ, the side door（24）With the tank body（5）Connected by hinge
It connects, the sealing strip（23）Material be quenched rubber, the sealing strip（23）Thickness be not less than 4mm.
7. a kind of bacterium automatic sorting labelling apparatus based on immunization method according to claim 1, it is characterised in that：Institute
State handle（25）Material be phenolic resin, the infrared camera（20）Resolution ratio be not less than 720P, the insulation board
（18）Material be silicon carbide, the insulation board（18）Thickness be not less than 5mm.
8. a kind of bacterium automatic sorting labelling apparatus based on immunization method according to claim 1, it is characterised in that：Institute
State side plate（17）Material be stainless steel, the side plate（17）For sieve texture, the side plate（17）Screen number be not less than
800, the limited block（22）Quantity be not less than four, in pairs exist, the electrostatic generator（16）Peak impulse voltage
No more than 120Kv.
9. a kind of bacterium automatic sorting labelling apparatus based on immunization method according to claim 1, it is characterised in that：Institute
State control host（10）Model PLC S7-1200, the display screen（6）Material be IPS, the control keyboard（11）It is interior
Embedded in the control host（10）On, the control host（10）With the display screen（6）, the servo motor（1）, the electricity
Magnet valve（15）, the electrostatic generator（16）, the flow sensor（19）, the infrared camera（20）With the control key
Disk（11）Electrical connection.
10. a kind of bacterium automatic sorting labeling method based on immunization method, it is characterised in that：Include the following steps：
（1）Nanogold is combined with antibody molecule, obtains the nanogold of antibody molecule, the antibody molecule energy in nanogold and mesh
Bacterium specific binding, while fluorescent material being combined with antibody molecule, obtains the fluorescent material of antibody molecule, fluorescence
Antibody molecule in matter can be specifically bound with purpose bacterium, and fluorescent material is that isosulfocyanic acid fluorescence is orange red, isosulfocyanic acid fluorescence
Huang, isosulfocyanic acid fluorescence is red, fluorescein isothiocynate isomers I, one kind in 5-isothiocyanate fluorescein；
（2）Inspection ensures the capping（13）It is fastened on the outlet tube（12）It is interior, while it is ensured that the side door（24）It is in
Sealing state is closed, the nanogold for having antibody molecule and there will be the fluorescent material of antibody molecule to be separately added into two storages later
Feed bin（4）It is interior, while analyte sample fluid is added to the tank body through the inlet tube（5）It is interior；
（3）The amount for the nanogold that antibody molecule need to be added is confirmed according to the amount of analyte sample fluid, passes through the control later
Keyboard（11）Control the solenoid valve（15）It opens, has the nanogold of antibody molecule through the discharge bucket（14）Into the tank body
（5）It is interior, while the flow sensor（19）Work obtains the discharge bucket in real time（14）The flow information at place is simultaneously back to institute
State control host（10）, the control host（10）By flow information be converted to after real-time load be preset with antibody molecule
Nanogold amount is compared, when identical as the nanogold amount of antibody molecule is preset with after real-time load, the solenoid valve
（15）It is automatically closed；
（4）Pass through the control keyboard（11）Control the servo motor（1）Work drives the shaft（9）Rotation, described turn
Axis（9）Drive the agitating roller（21）Rotation is by analyte sample fluid and has the nanogold of antibody molecule to stir combination, nanometer golden watch
The antibody molecule in face can be specifically bound with purpose bacterial surface antigen, to obtain the mixture of nanogold and purpose bacterium；
（5）Pass through the control keyboard（11）Control the electrostatic generator（16）Work generates electrostatic, and electrostatic is in the side plate
（17）On be uniformly distributed, the mixture of nanogold and purpose bacterium is attached to the side plate after Electrostatic Absorption（17）On, pass through
The agitating roller（21）Rotation makes nanogold and the mixture of purpose bacterium be substantially adhered to the side plate（17）Stop after upper；
（6）The amount for the fluorescent material that antibody molecule need to be added is confirmed according to the amount of analyte sample fluid, passes through the control later
Keyboard processed（11）Control the solenoid valve（15）It opens, has the fluorescent material of antibody molecule through the discharge bucket（14）Into described
Tank body（5）It is interior, while the flow sensor（19）Work obtains the discharge bucket in real time（14）The flow information at place and passback
To the control host（10）, the control host（10）By flow information be converted to after real-time load be preset with antibody point
Son fluorescence quality compared, after real-time load be preset with antibody molecule fluorescence it is identical in quality when, it is described
Solenoid valve（15）It is automatically closed；
（7）Close the electrostatic generator（16）, and control the agitating roller（21）It rotates to make to have the fluorescence of antibody molecule
Matter is fully combined with nanogold with the mixture of purpose bacterium, and the antibody molecule of phosphor surface can be thin with purpose with nanogold
The mixture surface antigen of bacterium is specifically bound, to obtain the mixture of fluorescent material and nanogold and purpose bacterium, in turn
Complete the sorting indicia to purpose bacterium；
（8）Start the electrostatic generator（16）Fluorescent material and nanogold and the mixture of purpose bacterium are inhaled by electrostatic afterwards
It is attached to the side plate（17）On, control the infrared camera（20）Work obtains regarding for bacterium sorting indicia working condition in real time
Frequency information and in display screen（6）Upper real-time display, by observing the display screen（6）Come determine the work of bacterium sorting indicia into
It spends, after the completion of sorting indicia, opens the end cap by analyte sample fluid through the outlet tube（12）Discharge, and open the side door
（24）By the side plate（17）It takes out and subsequent processing is carried out to purpose bacterium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810266026.9A CN108414752A (en) | 2018-03-28 | 2018-03-28 | A kind of bacterium automatic sorting labeling method and device based on immunization method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810266026.9A CN108414752A (en) | 2018-03-28 | 2018-03-28 | A kind of bacterium automatic sorting labeling method and device based on immunization method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108414752A true CN108414752A (en) | 2018-08-17 |
Family
ID=63132588
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810266026.9A Pending CN108414752A (en) | 2018-03-28 | 2018-03-28 | A kind of bacterium automatic sorting labeling method and device based on immunization method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108414752A (en) |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1477397A (en) * | 2002-08-23 | 2004-02-25 | � 赵 | Microbial body sorting and detection method, its special-purpose equipment and its kit |
| CN2693346Y (en) * | 2003-07-24 | 2005-04-20 | 王克成 | High pressure electrostatic separator |
| CN101462094A (en) * | 2007-12-18 | 2009-06-24 | 杨卫华 | Injection type electrostatic sorting method and apparatus |
| CN102243234A (en) * | 2011-04-11 | 2011-11-16 | 中国人民解放军军事医学科学院卫生装备研究所 | Automatic bacterial sorting and labeling method based on immune method, and apparatus thereof |
| CN202049159U (en) * | 2011-04-11 | 2011-11-23 | 中国人民解放军军事医学科学院卫生装备研究所 | Automatic bacterium sorting and labeling device based on immune method |
| CN105675864A (en) * | 2016-02-29 | 2016-06-15 | 董玉俊 | Automatic bacteria sorting and marking device based on immune method |
| CN107618132A (en) * | 2017-10-24 | 2018-01-23 | 佛山市三水兴达涂料有限公司 | A kind of environmental protection flame retardant Wood-plastic material processing unit (plant) and processing method |
| CN107718618A (en) * | 2017-11-12 | 2018-02-23 | 荣成荣鹰橡胶制品有限公司 | A kind of solid tyre production shaping forming device |
| CN207088236U (en) * | 2017-08-12 | 2018-03-13 | 长春市聚仁塑胶材料有限公司 | A kind of plastic grain agitating device |
| CN107827262A (en) * | 2017-11-23 | 2018-03-23 | 李良浩 | A kind of flat ceramic film water purifier and its purification technique |
-
2018
- 2018-03-28 CN CN201810266026.9A patent/CN108414752A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1477397A (en) * | 2002-08-23 | 2004-02-25 | � 赵 | Microbial body sorting and detection method, its special-purpose equipment and its kit |
| CN2693346Y (en) * | 2003-07-24 | 2005-04-20 | 王克成 | High pressure electrostatic separator |
| CN101462094A (en) * | 2007-12-18 | 2009-06-24 | 杨卫华 | Injection type electrostatic sorting method and apparatus |
| CN102243234A (en) * | 2011-04-11 | 2011-11-16 | 中国人民解放军军事医学科学院卫生装备研究所 | Automatic bacterial sorting and labeling method based on immune method, and apparatus thereof |
| CN202049159U (en) * | 2011-04-11 | 2011-11-23 | 中国人民解放军军事医学科学院卫生装备研究所 | Automatic bacterium sorting and labeling device based on immune method |
| CN105675864A (en) * | 2016-02-29 | 2016-06-15 | 董玉俊 | Automatic bacteria sorting and marking device based on immune method |
| CN207088236U (en) * | 2017-08-12 | 2018-03-13 | 长春市聚仁塑胶材料有限公司 | A kind of plastic grain agitating device |
| CN107618132A (en) * | 2017-10-24 | 2018-01-23 | 佛山市三水兴达涂料有限公司 | A kind of environmental protection flame retardant Wood-plastic material processing unit (plant) and processing method |
| CN107718618A (en) * | 2017-11-12 | 2018-02-23 | 荣成荣鹰橡胶制品有限公司 | A kind of solid tyre production shaping forming device |
| CN107827262A (en) * | 2017-11-23 | 2018-03-23 | 李良浩 | A kind of flat ceramic film water purifier and its purification technique |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US5536475A (en) | Apparatus for magnetic cell separation | |
| CN103604939B (en) | A kind of Full-automatic luminescent immunoassay system based on micronano-magnetic bead electromagnetic transfer technique | |
| CN104651315B (en) | It is a kind of to recognize the method that tumour cell is sorted with cell size difference using antigen and antibody specific simultaneously in micro-fluidic chip | |
| CN102243234B (en) | Automatic bacterial sorting and labeling method based on immune method, and apparatus thereof | |
| CN206008631U (en) | A kind of Agitation Tank for producing intensified loquet distillate | |
| CN106662596A (en) | Substrate for sample analysis, and sample analysis apparatus | |
| US7713752B2 (en) | Magnetic bead agglomerator for automated ELISA process | |
| CN108414752A (en) | A kind of bacterium automatic sorting labeling method and device based on immunization method | |
| CN206980610U (en) | Spiral ribbon mixer | |
| CN202049159U (en) | Automatic bacterium sorting and labeling device based on immune method | |
| CN104923395A (en) | Electromagnetic and electric integrated device for separating and transferring magnetic particles | |
| CN110066196A (en) | A kind of aquaculture waste is used for the fertilizer Zymolysis Equipment of hickory chick plantation | |
| CN203705459U (en) | Full-automatic luminescence immunoassay system based on micro-nano magnetic bead electromagnetic transfer technology | |
| CN106110716B (en) | A kind of microzooplankton settling separation device and separation method | |
| CN103852518B (en) | A kind of multichannel independent dominating set of based thin film acoustic wave device becomes micro-fluidic chip | |
| CN105435488B (en) | Unload column valve and containing automatic chromatographic apparatus and the device usage for unloading column valve | |
| CN106770269A (en) | A kind of Portable outdoor laboratory Water quality pH value efficient detection pen | |
| CN212800300U (en) | Detection equipment suitable for biological gene | |
| CN114935484A (en) | Low-carbon and high-efficiency field rapid enrichment and separation device and method for large-volume water sample | |
| CN206631254U (en) | One kind plus-minus pressure double-purpose operates desk-top chromatographic column | |
| CN107228943B (en) | The method for measuring bacterial content in fluid sample | |
| CN207941524U (en) | A kind of novel reaction kettle | |
| CN110160854A (en) | It is a kind of to keep the device of bead suspension uniformity, method and application thereof | |
| CN206990273U (en) | A kind of liquid layered accurate sampling device | |
| CN206199149U (en) | A kind of chemical experiment heating and stirring device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180817 |
|
| RJ01 | Rejection of invention patent application after publication |