CN108414544A - A kind of device of detection aquatic organism pharmacokinetics - Google Patents
A kind of device of detection aquatic organism pharmacokinetics Download PDFInfo
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- CN108414544A CN108414544A CN201810132854.3A CN201810132854A CN108414544A CN 108414544 A CN108414544 A CN 108414544A CN 201810132854 A CN201810132854 A CN 201810132854A CN 108414544 A CN108414544 A CN 108414544A
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- G01N23/02—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by transmitting the radiation through the material
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- G01N23/046—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by transmitting the radiation through the material and forming images of the material using tomography, e.g. computed tomography [CT]
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Abstract
The present invention provides a kind of device of detection aquatic organism pharmacokinetics, including shell, positioned at the first chamber and second chamber and separator, inlet, liquid outlet of the shell；Wherein, the inlet is connected to by first passage with the first chamber, and the first chamber is connected to by second channel with the second chamber, and the liquid outlet is connected to by third channel with the second chamber；The separator includes that component is isolated in the first isolation component, the second isolation component and third；The first isolation component is set between the inlet and the first chamber, and the second isolation component is set between the first chamber and the second chamber, and the third isolation component is set between the second chamber and the liquid outlet.
Description
Technical field
The present invention relates to biological detection structure more particularly to a kind of devices of detection aquatic organism pharmacokinetics.
Background technology
The Pharmacokinetic Assessments of aquatile are of great significance for what environmental assessment, aquatile were metabolized.
In the related technology, image checking is usually carried out to aquatile by fluorescence microscope imaging method, optoacoustic detection method, passes through monitoring
Aquatile is realized and comments the pharmacokinetics of aquatile to the dynamic absorption of special component (such as calcium ion)
Estimate.When carrying out image checking to aquatile using different methods, the requirement to the device for housing aquatile is not yet
It is identical to the greatest extent.
Positive electron reflection tomoscan (Positron Emission Tomography, PET) method has been applied at present
The three-dimensional imaging field of small-sized mammalian；But when being imaged and being detected to aquatile, due to maintaining aquatile life
The water environment deposited is free-moving, after the probe molecule of isotope labeling is entered in aquatile body, can be breathed due to gill formula,
The reasons such as excretion reveal isotope labeling molecule in water, it is difficult to distinguish radioactive activity signal in aquatile and water body.It answers
Image checking is carried out to aquatile with PET methods, is the dynamic imaging data based on specific species mark molecule, based on described
Dynamic imaging data is realized carries out Pharmacokinetic Assessments to aquatile.Although using PET methods to small-sized aquatic life
Object carries out the high sensitivity of image checking, but all to construction, performance and the processing request of the detection container of receiving aquatile
It is very high.Therefore, the detection container for aquatile image checking is met there is an urgent need for a kind of simple processing, performance and construction.
Invention content
To solve existing technical problem, an embodiment of the present invention provides a kind of detection aquatic organism pharmacokinetics
Device can at least solve the above-mentioned problems in the prior art.
An embodiment of the present invention provides a kind of devices of detection aquatic organism pharmacokinetics, including：Shell is located at institute
State the first chamber and second chamber and separator, inlet, liquid outlet of shell；Wherein,
The inlet is connected to by first passage with the first chamber, and the first chamber passes through second channel and institute
Second chamber connection is stated, the liquid outlet is connected to by third channel with the second chamber；
The separator includes that component is isolated in the first isolation component, the second isolation component and third；First insulator
Part is set between the inlet and the first chamber, it is described second isolation component be set to the first chamber with it is described
Between second chamber, the third isolation component is set between the second chamber and the liquid outlet；
Liquid after the first isolation component enters to the first chamber, is isolated by the inlet through described second
Component enters to the second chamber, and component most is isolated through the third afterwards and the liquid outlet flows out.
In said program, the shell includes：Matrix and upper cover；
In the upper surface of described matrix, opened up perpendicular to the direction of the upper surface fluted；The groove includes mutual
Parallel first side and second side, and the third side and the 4th side that are mutually parallel；Wherein,
The first side and the second side have the first height, and the third side and the 4th side have
Second height；First height is more than second height.
In said program, guiding groove is respectively arranged at the second height of the first side and the second side；
The guiding groove guides the upper cover to the groove is completely covered, and realizes the encapsulation to the shell.
In said program, the bottom surface of the groove offers at least one first chamber and at least one second chamber；
The first end face adjacent with the first side of the groove offers at least one inlet, it is described it is at least one into
Liquid mouth is connected to corresponding first chamber；
The second end face adjacent with the second side of the groove offers at least one liquid outlet, it is described it is at least one go out
Liquid mouth is connected to corresponding second chamber.
In said program, the first isolation component includes the first card slot and the first grid；
Two side nestings of first card slot through the first passage are to the first passage；
First grid intert realized to first card slot physics of the inlet and the first chamber every
From.
In said program, the shell includes：Axis body, the first packaging part and the second packaging part；
In the end face of the axis body, the first cavity is offered along the axis direction of the axis body, the both ends of the axis body are equal
It is provided with external screw thread；
First free end of first packaging part is provided with internal thread, the second free end setting of second packaging part
There is internal thread；
The sealed washer of internal thread of the internal thread of first packaging part and second packaging part respectively with the axis
The external screw thread at body both ends coordinates, and realizes the encapsulation to the shell.
In said program, the shell includes：External member, axis body, the first packaging part and the second packaging part；
The external member is provided at both ends with external screw thread, and the axis body is embedded in inside the external member；
In the end face of the axis body, the axis direction along the axis body offers cavity；
First free end of first packaging part is provided with internal thread, the second free end setting of second packaging part
There is internal thread；
The sealed washer of internal thread of the internal thread of first packaging part and second packaging part respectively with the set
The external screw thread at part both ends coordinates, and realizes the encapsulation to the shell.
In said program, the first isolation component be set to first packaging part and the cavity third end face it
Between；
The second isolation component is set between the third end face of the cavity and the 4th end face of the cavity, with shape
At first chamber and second chamber；
The third isolation component is set between the 4th end face and second packaging part.
In said program, the first cardinal extremity of first packaging part is provided with the first connector of protrusion, forms inlet；
Second cardinal extremity of second packaging part is provided with the second connector of protrusion, forms liquid outlet.
In said program, described device further includes fluid pump, and the fluid pump is to the liquid for carrying signal source molecule
Body exports the first power, so that the liquid flows into the first chamber through the inlet.
Implementing the device provided in an embodiment of the present invention for detecting aquatic organism pharmacokinetics includes：Shell is located at institute
State the first chamber and second chamber and separator, inlet, liquid outlet of shell；Wherein, the inlet is logical by first
Road is connected to the first chamber, and the first chamber is connected to by second channel with the second chamber, and the liquid outlet is logical
Third channel is crossed to be connected to the second chamber；The separator include first isolation component, second isolation component and third every
Ion part；The first isolation component is set between the inlet and the first chamber, and the second isolation component is set
Be placed between the first chamber and the second chamber, third isolation component be set to the second chamber with it is described go out
Between liquid mouth；The liquid for carrying signal source molecule enters to first chamber by the inlet through the first isolation component
Behind room, component is isolated through described second and enters to the zoic second chamber of receiving, component most is isolated through the third afterwards
And the liquid outlet outflow.In this way, being loaded with the liquid for carrying signal source molecule in the first chamber, removed in the second chamber
It is loaded with outside the liquid for carrying signal source molecule, also accommodates by organism；By in the first chamber and the second chamber
Between setting the second isolation component, realize physical isolation between the first chamber and the second chamber and described
Liquid communication between first chamber and the second chamber.
Description of the drawings
Fig. 1 is the composed structure signal of the device for the detection aquatic organism pharmacokinetics that the embodiment of the present invention one provides
Figure；
Fig. 2 is the structural schematic diagram of the shell for the device that the embodiment of the present invention one detects aquatic organism pharmacokinetics；
Fig. 3 is the composed structure signal of the device of detection aquatic organism pharmacokinetics provided by Embodiment 2 of the present invention
Figure；
Fig. 4 is the assembling schematic diagram of the device of detection aquatic organism pharmacokinetics provided by Embodiment 2 of the present invention；
Fig. 5 is the composed structure signal of the device for the detection aquatic organism pharmacokinetics that the embodiment of the present invention three provides
Figure；
Fig. 6 is the assembling schematic diagram of the device for the detection aquatic organism pharmacokinetics that the embodiment of the present invention three provides.
Specific implementation mode
Invention is further described in detail in the following with reference to the drawings and specific embodiments.It should be appreciated that described herein
Specific embodiment be only used to explain the present invention, be not intended to limit the present invention.
Embodiment one
Referring to Fig. 1, Fig. 1 is the composition of the device for the detection aquatic organism pharmacokinetics that the embodiment of the present invention one provides
Structural schematic diagram, including：
Shell 1 is located at the first chamber 2 and second chamber 3 and separator 4, inlet 5 and liquid outlet of the shell
6；Wherein,
The separator 4 includes that component 42 and third isolation component 43 is isolated in the first isolation component 41, second；
The first isolation component 41 is set between the inlet 5 and the first chamber 2, second insulator
Part 42 is set between the first chamber 2 and the second chamber 3, and the third isolation component 43 is set to second chamber
Between room 3 and the liquid outlet 6.
In one embodiment, the structural schematic diagram of shell, as shown in Fig. 2, including matrix 11 and upper cover 12；In described matrix
11 upper surface opens up fluted 111 perpendicular to the direction of the upper surface；The groove 111 includes first to be mutually parallel
Side 112 and second side 113, and the third side 114 and the 4th side 115 that are mutually parallel.
Wherein, 113 having the same first height of the first side 112 and the second side, the third side
114 and the height of the 4th side 115 having the same second, and first height is more than second height.
Here, it is respectively arranged with guiding groove at the second height of the first side 112 and the second side 113
116；The guiding groove 116 realizes the encapsulation to the shell for guiding the upper cover 12 that the groove 111 is completely covered.
At this point, liquid and biological quilt that liquid and the second chamber 3 that the first chamber 2 in the detection device accommodates accommodate
It encapsulates to the device of the detection aquatic organism pharmacokinetics, without leaking.
In one embodiment, the upper cover 12, which is cover type, can paste cap, that is to say, that at the bottom of the upper cover 12
Face is covered with viscose, and before covering the groove 111 using the upper cover 12, the viscose of the covered ground of the upper cover 12 is used
Viscose encapsulating film covers.When meeting the encapsulation condition of device of the detection aquatic organism pharmacokinetics, viscose is removed
The upper cover 12 is inserted into the guiding groove 116 by encapsulating film so that the upper cover 12 is under the guiding of the guiding groove, covering
The groove 111.
In another embodiment, the upper cover 12 can be also covered by way of rotation to the groove 111.Certainly, originally
The specific implementation that invention covers the upper cover 12 groove 111 does not limit, any to realize that upper cover 12 is covered
Cover the realization method of the groove 111 within protection scope of the present invention.
Here, the encapsulation condition of the device of the detection aquatic organism pharmacokinetics includes at least：Aquatic organism has been put
It is placed in the second chamber 3.
In one embodiment, it is offered at least in the first end face 117 adjacent with the first side 112 of the groove 111
One inlet 5, at least one inlet 5 are connected to corresponding first chamber 2.Here, the connection can be straight line
Connection can also be non-rectilinear connection.
At least one liquid outlet 6, institute are offered in the second end face 119 adjacent with the second side 113 of the groove 111
At least one liquid outlet 6 is stated to be connected to corresponding second chamber 3.Here, the connection can be straight line connection, can also right and wrong
Straight line is connected to.
In the embodiment of the present invention, the first isolation component 41, the second isolation component 42 and the third insulator
Part 43 can be identical structure.As shown in Figure 1, by taking the second isolation component 42 as an example, the first isolation component 42 wraps
Include the first card slot 421 and the first grid 422.
Two side nestings of first card slot 421 through the first passage 423 are described to the first passage 423
First passage 423 is the channel that the first chamber 2 is connected to the second chamber 3.
Second grid 422 interts to second card slot 421, so not only realizes the first chamber 2 and institute
The physical isolation between second chamber 3 is stated, and allows fluid to be flowed through second grid 422 by the first chamber 2
Enter the second chamber 3, that is, realizes the liquid communication between the first chamber 2 and the first chamber 3.
Correspondingly, the structure and work(of the first isolation component 41 described in the embodiment of the present invention and third isolation component 43
It can be same or similar.The first isolation component 41 not only realizes the physics between the first chamber 2 and the inlet 5
Isolation, and allow fluid to that 41 corresponding grid of component inflow, first chamber is isolated through described first by the inlet
Room 2.
Correspondingly, the third isolation component 43 not only realizes the object between the second chamber 3 and the liquid outlet 6
Reason isolation, and the liquid in the second chamber 3 is enable to flow into institute through third isolation 43 corresponding grid of component
Liquid outlet 6 is stated, realizes the recycling of liquid.
Using the embodiment of the present invention it is shown in FIG. 1 detection aquatic organism pharmacokinetics device carry out aquatile at
When as detection, the device of the detection aquatic organism pharmacokinetics is positioned over to the chamber of position emissron tomography imager
It is interior；The liquid for carrying signal source molecule enters to the first chamber 2 by the inlet 5 through the first isolation component 41
Afterwards, through it is described second be isolated component 42 enter to the second chamber 3, most afterwards through the third be isolated component 43 and it is described go out liquid
Mouth 6 flows out.In this detection process, living body biological to be detected, such as zebra fish, first chamber 2 are equipped in second chamber 3
Identical with the ambient environmental conditions of second chamber 3, first chamber 2 is identical with the liquid component in second chamber 3, therefore,
It regard first chamber 2 as sample chamber as chamber, second chamber 3 is referred to, by by the testing result of second chamber 3 and first
The testing result of chamber 2 is compared, and absorption and metabolic condition of the biology to be detected to signal source molecule is obtained, to realize to life
The assessment of the pharmacokinetics of object.
Here, the signal source molecule is the molecule or ion of label, such as calcium ion.
The device of detection aquatic organism pharmacokinetics shown in FIG. 1 is array detection structure, including three groups of independences
Biological detection structure a, b and c；When the device of the detection aquatic organism pharmacokinetics is applied to utilize PET methods pair
When aquatile carries out image checking, every group of independent biological detection structure is used equally for as utilization PET methods to aquatic life
Object carries out the container for accommodating aquatile when image checking.Therefore, detection aquatic organism pharmacokinetics shown in FIG. 1
Device can be achieved at the same time carries out image checking to three kinds of aquatiles using PET methods；When three kinds of aquatiles are one species
When, using the device of detection aquatic organism pharmacokinetics shown in FIG. 1, the inspection that can will be obtained using three groups of detection structures
It surveys result to be averaged, obtains the testing result of the type aquatile；It so, it is possible to reduce detection error, improve detection
Precision.When three kinds of aquatiles be variety classes when, using it is shown in FIG. 1 detection aquatic organism pharmacokinetics device,
Can three kinds of different types of aquatiles be carried out while be detected simultaneously, saved the time of biological detection, improve biology
The efficiency of detection.
It should be noted that the device of detection aquatic organism pharmacokinetics shown in FIG. 1 includes three groups of independent lifes
Quality testing geodesic structure is only an example, in practical applications, can flexibly change detection aquatic organism drug metabolism as needed
The quantity for the independent biological detection structure that dynamic (dynamical) device includes.Certainly, detection aquatic organism drug metabolism shown in FIG. 1 is dynamic
The device of mechanics in addition to be applied to using PET methods to biology carry out image checking, apply also for other can to biology into
The method of row image checking.
Embodiment two
The present invention shown in Figure 3 implements the composed structure of the device for the detection aquatic organism pharmacokinetics that two provide
The present invention shown in schematic diagram and Fig. 4 implements the assembling signal of the device for the detection aquatic organism pharmacokinetics that two provide
Figure, the device for detecting aquatic organism pharmacokinetics include：
Shell 01 is located at the first chamber 02 of the shell and second chamber 03 and separator 04, inlet 05 and goes out
Liquid mouth 06；Wherein,
The separator 04 includes：Component 042 is isolated for first isolation component 041, second and component 043 is isolated in third；
It is described first isolation component 041 is set between the inlet 05 and the first chamber 02, described second every
Ion part 042 is set between the first chamber 02 and the second chamber 03, and the third isolation component 043 is set to institute
It states between second chamber 03 and the liquid outlet 06.
In one embodiment, the shell 01 includes：Axis body 011, the first packaging part 012, the second packaging part 013 and sealing
Washer 014；
In the end face of the axis body 011, cavity 0111 is offered along the axis direction of the axis body 011, in the axis body
011 both ends are both provided with external screw thread.
First free end 0121 of first packaging part 012 is provided with internal thread, and the second of second packaging part 013
Free end 0131 is provided with internal thread.
The sealed washer 014 of the internal thread of the internal thread of first packaging part 012 and second packaging part 013 is distinguished
Coordinate with the external screw thread at 011 both ends of the axis body, realizes the encapsulation to the shell 01.
In one embodiment, the first isolation component 041 is set to first packaging part 012 and the cavity 0111
Third end face 0112 between；
The second isolation component 042 is set to the of the third end face 0112 of the cavity 0111 and the cavity 0111
Between four end faces 0113, to form first chamber 02 and second chamber 03；
The third is set to the 4th end face 0113 of the cavity 0111 and second packaging part every sub- off member 043
Between 013.
In the embodiment of the present invention, the first isolation component 041 realizes the inlet 05 and the first chamber 02
Between physical isolation, and allow fluid to component 041 is isolated through described first by the inlet 05 and flow into described the
One chamber 02, that is, realize the liquid communication between the inlet 05 and the first chamber 02.
Correspondingly, described in the embodiment of the present invention second isolation component 042 and the third isolation component 043 structure and
Function is same or similar.The second isolation component 042 not only realize the first chamber 02 and the second chamber 03 it
Between physical isolation, and allow fluid to by the first chamber 02 through it is described second be isolated 042 corresponding grid of component
Flow into the second chamber 03.
Correspondingly, the third isolation component 043 not only realizes between the second chamber 03 and the liquid outlet 06
Physical isolation, and enable the liquid in the second chamber 03 that 043 corresponding grid of component is isolated through the third
The liquid outlet 06 is flowed into, realizes the recycling of liquid.
The function of the first isolation component 041 and third isolation component 043 can be realized by nozzle grid.Described second
The function of isolation component 042 can be realized by grid.
In one embodiment, the first cardinal extremity 0123 of first packaging part 012 is provided with the first connector of protrusion
0122, form inlet；First connector 0122 can be connect by pipeline with fluid source device.
Second cardinal extremity 0133 of second packaging part 013 is provided with the second connector 0132 of protrusion, forms liquid outlet；
Second connector 0132 can be connect by pipeline with fluid recycling apparatus, realize time of the liquid to being flowed out through liquid outlet
It receives.
In the embodiment of the present invention, the function of first packaging part 012 and second packaging part 013 can be by rotation gate valve
Encapsulation caps are realized.The function of first connector 0122 and second connector 0132 can be realized by female Luer.Pass through
The internal thread of first packaging part 012 and second packaging part 013 is matched with the external screw thread at 011 both ends of the axis body, real
Now to the encapsulation of the shell.At this point, liquid that first chamber 2 in the detection device accommodates and the second chamber
3 liquid accommodated and biology are packaged into the device of the detection aquatic organism pharmacokinetics, without leaking.
It is carried out using the device of Fig. 3 of the embodiment of the present invention and detection aquatic organism pharmacokinetics shown in Fig. 4 aquatic
When bio-imaging detects, the device of the detection aquatic organism pharmacokinetics is positioned over position emissron tomography imager
Chamber in；Carry the liquid of signal source molecule by the inlet 05 through described first be isolated component 041 enter to it is described
After first chamber 02, component 042 is isolated through described second and enters to the second chamber 3, component most is isolated through the third afterwards
043 and the liquid outlet 06 flow out.In this detection process, living body biological to be detected is equipped in second chamber 03, such as
Zebra fish, first chamber 02 is identical with the ambient environmental conditions of second chamber 03, the liquid in first chamber 02 and second chamber 03
Body ingredient is identical, therefore, regard first chamber 02 as sample chamber as chamber, second chamber 03 is referred to, by by the
The testing result of two chambers 03 is compared with the testing result of first chamber 02, obtains biology to be detected to signal source molecule
Absorption and metabolic condition, to realize the assessment of the pharmacokinetics to biology.
Here, the signal source molecule is the molecule or ion of label, such as calcium ion.
The device of Fig. 3 and detection aquatic organism pharmacokinetics shown in Fig. 4, are applied to using PET methods to aquatic
, it can be achieved that the image checking biological to one when biology progress image checking.It is of course also possible to be arranged multiple such as Fig. 3 and Fig. 4
The device of aquatic organism pharmacokinetics is detected, the apparatus array of detection aquatic organism pharmacokinetics is constituted, detects water
The device of multiple detection aquatic organism pharmacokinetics in the apparatus array of bio-pharmaceutical dynamic metabolism is in similarity condition
Environment in place, be such as placed in parallel.This row of the device of detection aquatic organism pharmacokinetics can be achieved at the same time to a variety of water
Raw biological utilisation PET methods carry out image checking；When a variety of aquatiles are one species, the detection aquatic organism drug metabolism
The testing result obtained using multigroup detection device, can be averaged, it is aquatic to obtain the type by this row of dynamic (dynamical) device
The testing result of biology；It so, it is possible to reduce detection error, improve the precision of detection.When a variety of aquatiles are variety classes
When, using the biological detection arrays, can a variety of different types of aquatiles be carried out while be detected simultaneously, save biology
The time of detection improves the efficiency of biological detection.
It, can be according to practical application, the quantity of unrestricted choice separator and combination, in the shell in the embodiment of the present invention
It is interior to form multiple chambers.
Embodiment three
The present invention shown in Figure 5 implements the composed structure of the device for the detection aquatic organism pharmacokinetics that three provide
The present invention shown in schematic diagram and Fig. 6 implements the assembling signal of the device for the detection aquatic organism pharmacokinetics that three provide
Figure, the device for detecting aquatic organism pharmacokinetics include：
Shell 01 is located at the first chamber 02 of the shell and second chamber 03 and separator 04, inlet 05 and goes out
Liquid mouth 06；Wherein,
The separator 04 includes：Component 042 is isolated for first isolation component 041, second and component 043 is isolated in third；
It is described first isolation component 041 is set between the inlet 05 and the first chamber 02, described second every
Ion part 042 is set between the first chamber 02 and the second chamber 03, and the third isolation component 043 is set to institute
It states between second chamber 03 and the liquid outlet 06.
In one embodiment, the shell 01 includes：Axis body 011, the first packaging part 012, the second packaging part 013, sealing
Washer 014 and external member 015；
The external member 015 is provided at both ends with external screw thread, and the axis body 011 is embedded in the inside of the external member 015；
In the end face of the axis body 011, cavity 0111 is offered along the axis direction of the axis body 011.
First free end 0121 of first packaging part 012 is provided with internal thread, and the second of second packaging part 013
Free end 0131 is provided with internal thread.
The sealed washer 014 of the internal thread of the internal thread of first packaging part 012 and second packaging part 013 is distinguished
Coordinate with the external screw thread at 014 both ends of the external member, realizes the encapsulation to the shell 01.
In one embodiment, the first isolation component 041 is set to first packaging part 012 and the cavity 0111
Third end face 0112 between；
The second isolation component 042 is set to the of the third end face 0112 of the cavity 0111 and the cavity 0111
Between four end faces 0113, to form first chamber 02 and second chamber 03；
The third is set to the 4th end face 0113 of the cavity 0111 and second packaging part every sub- off member 043
Between 013.
In the embodiment of the present invention, the first isolation component 041 realizes the inlet 05 and the first chamber 02
Between physical isolation, and allow fluid to component 041 is isolated through described first by the inlet 05 and flow into described the
One chamber 02, that is, realize the liquid communication between the inlet 05 and the first chamber 02.
Correspondingly, described in the embodiment of the present invention second isolation component 042 and the third isolation component 043 structure and
Function is same or similar.The second isolation component 042 not only realize the first chamber 02 and the second chamber 03 it
Between physical isolation, and allow fluid to by the first chamber 02 through it is described second be isolated 042 corresponding grid of component
Flow into the second chamber 03.
Correspondingly, the third isolation component 043 not only realizes between the second chamber 03 and the liquid outlet 06
Physical isolation, and enable the liquid in the second chamber 03 that 043 corresponding grid of component is isolated through the third
The liquid outlet 06 is flowed into, realizes the recycling of liquid.
The function of the first isolation component 041 and third isolation component 043 can be realized by nozzle grid.Described second
The function of isolation component 042 can be realized by grid.
In one embodiment, the first cardinal extremity 0123 of first packaging part 012 is provided with the first connector of protrusion
0122, form inlet；First connector 0122 can be connect by pipeline with fluid source device.
Second cardinal extremity 0133 of second packaging part 013 is provided with the second connector 0132 of protrusion, forms liquid outlet；
Second connector 0132 can be connect by pipeline with fluid recycling apparatus, realize time of the liquid to being flowed out through liquid outlet
It receives.
In the embodiment of the present invention, the function of first packaging part 012 and second packaging part 013 can be by rotation gate valve
Encapsulation caps are realized.The function of first connector 0122 and second connector 0132 can be realized by female Luer.Pass through
The internal thread of first packaging part 012 and second packaging part 013 is matched with the external screw thread at 011 both ends of the axis body, real
Now to the encapsulation of the shell.At this point, liquid that first chamber 2 in the detection device accommodates and the second chamber
3 liquid accommodated and biology are packaged into the device of the detection aquatic organism pharmacokinetics, without leaking.
It is carried out using the device of Fig. 5 of the embodiment of the present invention and detection aquatic organism pharmacokinetics shown in fig. 6 aquatic
When bio-imaging detects, the device of the detection aquatic organism pharmacokinetics is positioned over position emissron tomography imager
Chamber in；Carry the liquid of signal source molecule by the inlet 05 through described first be isolated component 041 enter to it is described
After first chamber 02, component 042 is isolated through described second and enters to the second chamber 3, component most is isolated through the third afterwards
043 and the liquid outlet 06 flow out.In this detection process, living body biological to be detected is equipped in second chamber 03, such as
Zebra fish, first chamber 02 is identical with the ambient environmental conditions of second chamber 03, the liquid in first chamber 02 and second chamber 03
Body ingredient is identical, therefore, regard first chamber 02 as sample chamber as chamber, second chamber 03 is referred to, by by the
The testing result of two chambers 03 is compared with the testing result of first chamber 02, obtains biology to be detected to signal source molecule
Absorption and metabolic condition, to realize the assessment of the pharmacokinetics to biology.
Here, the signal source molecule is the molecule or ion of label, such as calcium ion.
The device of Fig. 5 and detection aquatic organism pharmacokinetics shown in fig. 6, are applied to using PET methods to aquatic
, it can be achieved that the image checking biological to one when biology progress image checking.It is of course also possible to be arranged multiple such as Fig. 5 and Fig. 6
The device of aquatic organism pharmacokinetics is detected, the apparatus array of detection aquatic organism pharmacokinetics is constituted, detects water
The device of multiple detection aquatic organism pharmacokinetics in the apparatus array of bio-pharmaceutical dynamic metabolism is in similarity condition
Environment in place, be such as placed in parallel.This row of the device of detection aquatic organism pharmacokinetics can be achieved at the same time to a variety of water
Raw biological utilisation PET methods carry out image checking；When a variety of aquatiles are one species, the detection aquatic organism drug metabolism
The testing result obtained using multigroup detection device, can be averaged, it is aquatic to obtain the type by this row of dynamic (dynamical) device
The testing result of biology；It so, it is possible to reduce detection error, improve the precision of detection.When a variety of aquatiles are variety classes
When, using the biological detection arrays, can a variety of different types of aquatiles be carried out while be detected simultaneously, save biology
The time of detection improves the efficiency of biological detection.
It, can be according to practical application, the quantity of unrestricted choice separator and combination, in the shell in the embodiment of the present invention
It is interior to form multiple chambers.
Example IV
Based on above-described embodiment one, embodiment two and embodiment three, it is aquatic that the embodiment of the present invention four also provides a kind of detection
The device of object pharmacokinetics, the device for the detection aquatic organism pharmacokinetics that the embodiment of the present invention four provides is upper
Further include fluid pump on the basis of stating embodiment one or embodiment two or embodiment three, the fluid pump is carried to described
The liquid of signal source molecule exports the first power, so that the liquid flows into the first chamber through the inlet.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
Those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, and should all contain
Lid is within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (10)
1. a kind of device of detection aquatic organism pharmacokinetics, which is characterized in that including：Shell is located at the shell
First chamber and second chamber and separator, inlet, liquid outlet；Wherein,
The inlet is connected to by first passage with the first chamber, and the first chamber passes through second channel and described the
Two chambers, the liquid outlet are connected to by third channel with the second chamber；
The separator includes that component is isolated in the first isolation component, the second isolation component and third；The first isolation component is set
It is placed between the inlet and the first chamber, the second isolation component is set to the first chamber and described second
Between chamber, the third isolation component is set between the second chamber and the liquid outlet；
After the first isolation component enters to the first chamber, component is isolated through described second by the inlet in liquid
The second chamber is entered to, component most is isolated through the third afterwards and the liquid outlet flows out.
2. the apparatus according to claim 1, which is characterized in that the shell includes：Matrix and upper cover；
In the upper surface of described matrix, opened up perpendicular to the direction of the upper surface fluted；The groove includes being mutually parallel
First side and second side, and the third side and the 4th side that are mutually parallel；Wherein,
There is the first height, the third side and the 4th side to have second for the first side and the second side
Highly；First height is more than second height.
3. the apparatus of claim 2, which is characterized in that second in the first side and the second side is high
Guiding groove is respectively arranged at degree；
The guiding groove guides the upper cover to the groove is completely covered, and realizes the encapsulation to the shell.
4. device according to claim 3, which is characterized in that the bottom surface of the groove offers at least one first chamber
With at least one second chamber；
The first end face adjacent with the first side of the groove offers at least one inlet, at least one inlet
It is connected to corresponding first chamber；
The second end face adjacent with the second side of the groove offers at least one liquid outlet, at least one liquid outlet
It is connected to corresponding second chamber.
5. the apparatus according to claim 1, which is characterized in that the first isolation component includes the first card slot and the first grid
Lattice；
Two side nestings of first card slot through the first passage are to the first passage；
First grid interts to first card slot, realizes the physical isolation of the inlet and the first chamber.
6. the apparatus according to claim 1, which is characterized in that the shell includes：Axis body, the first packaging part and the second envelope
Piece installing；
In the end face of the axis body, the first cavity is offered along the axis direction of the axis body, the both ends of the axis body are respectively provided with
There is external screw thread；
First free end of first packaging part is provided with internal thread, in the second free end of second packaging part is provided with
Screw thread；
The sealed washer of internal thread of the internal thread of first packaging part and second packaging part respectively with the axis body two
The external screw thread at end coordinates, and realizes the encapsulation to the shell.
7. the apparatus according to claim 1, which is characterized in that the shell includes：External member, axis body, the first packaging part and
Second packaging part；
The external member is provided at both ends with external screw thread, and the axis body is embedded in inside the external member；
In the end face of the axis body, the axis direction along the axis body offers cavity；
First free end of first packaging part is provided with internal thread, in the second free end of second packaging part is provided with
Screw thread；
The sealed washer of internal thread of the internal thread of first packaging part and second packaging part respectively with the external member two
The external screw thread at end coordinates, and realizes the encapsulation to the shell.
8. the device described according to claim 6 or 7, which is characterized in that the first isolation component is set to first envelope
Between piece installing and the third end face of the cavity；
The second isolation component is set between the third end face of the cavity and the 4th end face of the cavity, to form the
One chamber and second chamber；
The third isolation component is set between the 4th end face and second packaging part.
9. the device described according to claim 6 or 7, which is characterized in that the first cardinal extremity of first packaging part is provided with convex
The first connector gone out forms inlet；
Second cardinal extremity of second packaging part is provided with the second connector of protrusion, forms liquid outlet.
10. the apparatus according to claim 1, which is characterized in that described device further includes fluid pump, and the fluid pump is to institute
Liquid the first power of output for carrying signal source molecule is stated, so that the liquid flows into first chamber through the inlet
Room.
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