CN108414333B - Device for sample dyeing module on automatic slice dyeing machine - Google Patents
Device for sample dyeing module on automatic slice dyeing machine Download PDFInfo
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- CN108414333B CN108414333B CN201810210860.6A CN201810210860A CN108414333B CN 108414333 B CN108414333 B CN 108414333B CN 201810210860 A CN201810210860 A CN 201810210860A CN 108414333 B CN108414333 B CN 108414333B
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- 238000004043 dyeing Methods 0.000 title claims abstract description 32
- 238000001816 cooling Methods 0.000 claims abstract description 51
- 239000007788 liquid Substances 0.000 claims abstract description 44
- 238000010438 heat treatment Methods 0.000 claims abstract description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 21
- 239000000110 cooling liquid Substances 0.000 claims description 15
- 239000002699 waste material Substances 0.000 claims description 11
- 238000010186 staining Methods 0.000 claims description 10
- 230000005540 biological transmission Effects 0.000 claims description 8
- 239000000427 antigen Substances 0.000 abstract description 20
- 102000036639 antigens Human genes 0.000 abstract description 20
- 108091007433 antigens Proteins 0.000 abstract description 20
- 238000001514 detection method Methods 0.000 abstract description 5
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention relates to a device for loading a sample dyeing module on an automatic dyeing machine, which comprises a shell with a shell cover, wherein the shell cover is hinged with the shell through a connecting shaft; a plurality of liquid storage tanks are arranged in the shell; a heating device is arranged at the bottom of the shell; the cooling device comprises a fan and a circulating water cooling system; the fan is arranged below the shell, and the bottom of the shell is provided with a groove of which the central line is a curve; the circulating water cooling system is respectively arranged on the side wall and the bottom of the shell; the control unit is respectively connected with the driving device, the drain valve, the heating device and the fan through signals. According to the technical scheme, the circulating water cooling systems are arranged on the side wall and the bottom of the shell, so that the temperature in the shell can be kept constant as the set temperature, the tissue cell morphology of the biological sample after antigen restoration is improved, and the accuracy and the reliability of pathological detection are improved; when the antigen is repaired and needs to be cooled, the cooling speed can be increased, and the dyeing time can be shortened.
Description
Technical Field
The invention belongs to the field of medical detection instruments, particularly relates to the technical field of immunohistochemistry and immunocytochemistry dyeing, and more particularly relates to a device for a sample dyeing module on an automatic slide staining machine.
Background
Immunohistochemistry (IHC) is a new technology developed by combining the theory and technology of immunology on histochemical methods, and combines the theory and method of the subjects of immunology, histology and biochemistry, etc. The core of immunology, namely the principle of specific combination of antigen and antibody, is utilized, the antigen is tracked by using the antibody marked with enzyme, metal ion, isotope and the like, a display agent marked on the specific antibody combined with the antigen shows a certain color through chemical reaction, and the dyeing result is observed by means of a microscope, a fluorescence microscope and an electron microscope, so that the purpose of detecting the antigen is achieved.
Immunohistochemistry has a long history, and since 1940 Coons established immunofluorescence techniques to detect the corresponding antigens in frozen sections, there has been 73 history to date. In 1965, the immunohistochemical technique was first established in China, but the method was not widely applied to pathological diagnosis until the early 90 s in the 20 th century. The technique goes through 4 stages of development: establishing and developing an immunohistochemical technology; a popularization stage; clinical pathological diagnosis and application stage; quality control, normalization and standardization phases. Today, without the aid of immunohistochemistry, many tumors are poorly diagnosed and classified. Meanwhile, the wide application of immunohistochemistry in clinical pathological diagnosis also promotes the rapid development of immunohistochemical technology.
Since 1950, Journal of Histochemistry & Cytochemistry published a large number of documents of new technologies related to histomorphosis, such as enzyme-labeled immunohistochemistry, avidin biotin detection system, casein signal amplification system, and the like. All of these valuable findings have prompted the development of clinical and basic biomedical research worldwide. Immunohistochemistry has been widely used and accepted, and has become an indispensable link in pathological diagnosis. Immunohistochemistry not only improves the accuracy of pathological diagnosis, but also permeates basic and clinical subjects, and plays an immeasurable role in discussing etiology and pathogenesis of diseases and scientific research work.
The main steps in the immunohistochemical staining process include: baking, deparaffinization, hydration, antigen retrieval, or peroxidase blocking (optionally added), primary, secondary or tertiary antibody (optionally added), DAB or hematoxylin (optionally added), and all rinses.
With the development of science and technology, various advanced technologies are also gradually applied to the immunohistochemical automatic staining process to replace manual staining.
In each step of immunohistochemical staining, the most difficult is the antigen retrieval process, because the most common fixative for tissue fixation is formaldehyde, which will cross-link with proteins during tissue fixation to form a methylene-bridged cross-linked structure, thereby shielding the antigenic determinants in the tissue. Professor litholytica found that heating disrupted the protein cross-linking by formaldehyde fixation, re-exposing the masked antigenic determinants, and allowing binding to the corresponding antibodies.
At present, the antigen retrieval mode and the reagent type selection in the manual immunohistochemical staining process are flexible, and the common heat-guided antigen retrieval modes comprise: high pressure, water boiling, microwave, water bath, etc.; commonly used antigen retrieval solutions are citric acid (pH6.0), EDTA (pH9.0), Tris-HCl (pH 9.0-10). Different laboratories have different links such as detection personnel, selection of heating devices, control of heating temperature and time and the like, so that the immunohistochemical staining result inevitably has difference.
In order to overcome the difference of artificial dyeing results, an immunohistochemical automatic dyeing system is developed, and in recent years, full-automatic biochemical analyzers with different models and functions are in succession, so that the working efficiency and the detection accuracy are greatly improved. However, the heating temperature of the antigen repairing solution is lower than 100 ℃ for antigen repairing in the current full-automatic staining instrument in the market, and although manufacturers expect high-temperature repairing by adding high-boiling-point substances into the antigen repairing solution, in the antigen repairing process, after heating, the glass slide needs to be washed after cooling, in order to increase the cooling rate, the cooling speed is increased by a fan, but when the fan cools, the drying phenomenon occurs on the surface of a tissue slice on the glass slide, and the effect of combining with a corresponding antibody is influenced.
Disclosure of Invention
The invention aims to provide a device for a biological sample staining module on a glass slide, which aims to solve the problems that the cooling time is long after the antigen retrieval is finished, and the tissue cell morphology is changed due to the long cooling time, so that the pathological diagnosis is influenced in the prior art.
The invention is realized by the following technical scheme:
a device for loading a sample dyeing module on an automatic slice dyeing machine comprises a shell with a shell cover, wherein the shell cover is hinged with the shell through a connecting shaft; a plurality of liquid storage tanks are arranged in the shell;
the output shaft of the driving device transmits power to the connecting shaft so as to control the opening and closing of the shell cover;
a drainage tank is arranged in the shell and is communicated with each liquid storage tank; a drain hole is formed in the shell, one end of the drain hole is communicated with the drain groove, and the other end of the drain hole is opened on the outer side of the shell and is connected with a drain valve;
the heating device is arranged at the bottom of the shell;
the cooling device comprises a fan and a circulating water cooling system; the fan is arranged below the shell; the circulating water cooling system is respectively arranged on the side wall and the bottom of the shell;
wind shields extending downwards are arranged on three peripheries of the periphery of the bottom of the shell; at least more than two grooves are formed in the outer surface of the bottom of the shell, and one end of each groove is communicated with the periphery of the bottom of the shell, which is not provided with the wind shield; the central line of each groove is a curve;
and the control unit is respectively in signal connection with the driving device, the drain valve, the heating device and the fan.
A drainage plate and a waste liquid groove are arranged on the inner side of the shell cover, the drainage plate is obliquely arranged, and one side, close to the waste liquid groove, of the drainage plate is lower;
the waste liquid groove is communicated with the outer surface of the shell cover.
The output shaft of the driving device and the connecting shaft are in any one of belt transmission, chain transmission, gear transmission or hydraulic transmission.
The driving device is a motor, and the motor is fixed on the shell.
And a temperature sensor is arranged in the shell and is in signal connection with the control unit.
And a wind shield is arranged between two adjacent module grooves.
The circulating water cooling system is respectively arranged in the side wall of the shell and the bottom of the shell; a liquid inlet and a liquid outlet are arranged on the outer side of the shell.
The liquid inlet is connected with a cooling liquid pump through a pipeline; the liquid outlet is connected with a radiator through a pipeline, and the radiator is connected with the cooling liquid pump through a pipeline;
the cooling liquid pump is in signal connection with the control unit.
The axial centerline of the fan coincides with the housing bottom centerline.
The invention has the beneficial effects that:
according to the technical scheme, the circulating water cooling systems are arranged in the side wall and the bottom of the shell, the temperature in the shell can be detected through the temperature sensor of the shell, the circulating water cooling system is started at any time through the control unit, the soaking time of the antigen repairing liquid is shortened, the tissue cell morphology of a biological sample after antigen repairing is improved, and the accuracy and the reliability of pathological diagnosis are improved. .
On the other hand, through circulating water cooling system, when antigen retrieval finishes and needs cooling, can accelerate the cooling rate, shorten the dyeing time.
On the other hand, this technical scheme is through trilateral deep bead that is provided with in casing bottom to be provided with the central line on the bottom surface of casing and be curved recess, and do not set up the one side of deep bead in the bottom of casing through the recess opening, make the fan blow the air current flow direction and the velocity of flow of casing bottom all increase, improved the cooling effect of fan.
Drawings
FIG. 1 is a schematic view of a dyeing module according to the present invention;
FIG. 2 is a front view of a staining module of the invention;
FIG. 3 is a bottom view of the staining module of the present invention;
FIG. 4 is a cross-sectional view A-A of FIG. 2;
fig. 5 is a bottom view of the staining module with the fan removed.
Description of the reference numerals
The device comprises a shell body 1, a shell cover 2, a flow guide plate 3, a sealing ring 4, a connecting shaft 5, a supporting table 6, a wind shield 7, a slicing frame 8, an output shaft 9, a limiter 10, a motor 11, a fan 12, a drain hole 13, a drain valve 14, an electric heating plate 15, a circulating water cooling system 16, a cooling flow passage 161, a liquid inlet 17, a liquid outlet 18, a wind shield 20 and a groove 21.
Detailed Description
The technical solutions of the present invention are described in detail below by examples, and the following examples are only exemplary and can be used only for explaining and explaining the technical solutions of the present invention, but not construed as limiting the technical solutions of the present invention.
As shown in fig. 1, a housing 1 with a housing cover 2 is provided, the housing cover 2 is hinged to the housing 1 through a connecting shaft 5, and the housing cover can be opened or closed through the rotation of the connecting shaft, so that the dyeing module can be operated in each immunohistochemical process.
The dyeing module device belongs to a part of an automatic slice dyeing machine, and in the technical scheme of the application, only the dyeing module is improved, and the rest parts of the automatic slice dyeing machine still belong to the prior art, so that the structure of the automatic slice dyeing machine is not explained in the application.
The specific structure of the dyeing module device is as shown in fig. 1, a plurality of liquid storage tanks for storing reagents are arranged in a shell 1, the liquid storage tanks are arranged side by side, a drainage tank is arranged on one side opposite to the liquid storage tanks in the shell, and each liquid storage tank is communicated with the drainage tank, so that liquid in each liquid storage tank can be quickly drained through the drainage tank. A drain hole 13 is arranged at the bottom of the shell opposite to the drain groove, as shown in fig. 2 and fig. 3, the drain hole 13 is connected with a drain valve 14, the drain valve 14 is in signal connection with the control unit, the control unit outputs an opening signal to the drain valve 14, and the drain valve 14 opens the drain valve 14 after receiving the opening signal, so that liquid in the drain groove is drained.
A supporting platform 6 for placing the glass slide is arranged in each liquid storage tank, the supporting platform 6 is used for placing a slicing frame 8, and clamping devices which correspond to the liquid storage tanks one by one are arranged on the slicing frame 8. In the present application, the clamping device is prior art.
As shown in fig. 2, a driving device is disposed on a side surface of the housing 1, in this embodiment, the driving device is a motor 11, an output shaft 9 of the motor 11 is connected to the connecting shaft through a belt, a chain, a gear, or hydraulic pressure, and when the motor operates, the connecting shaft is driven to rotate, so as to open or close the housing cover.
And the shell 1 and the shell cover 2 are provided with a limiter 10, the limiter 10 is used for controlling the opening and closing amount of the shell cover, and the shell cover is provided with a sealing ring 4 for sealing with the shell.
The inner side of the shell cover 2 is provided with a drainage plate 3 and a waste liquid groove, and the drainage plate 3 is obliquely arranged and one side close to the waste liquid groove is lower; so that the condensed water flows toward the waste liquid tank through the liquid guide plate 3.
The waste liquid tank is communicated with the outer surface of the shell cover and discharges condensed water in the waste liquid tank out of the shell.
As shown in fig. 3 and 5, a heating device is disposed on the dyeing module, in this embodiment, the heating device is disposed at the bottom of the housing 1, and the heating device is an electric heating plate 15, the heating device can be disposed on the inner surface of the bottom of the housing and can be attached to the outer surface of the bottom of the housing (in this embodiment, the heating device is disposed on the outer surface of the bottom of the housing), the heating device is in signal connection with the control unit, the control unit controls the heating device to heat or stop heating according to a set program, and specifically controls when the heating device is turned on, for example, by a temperature sensor disposed in the housing, and a contact sensor disposed between the housing and the housing cover, for example, when the heating device.
The dyeing module comprises a cooling device, as shown in fig. 2 to 5, wherein the cooling device comprises a fan 12 and a circulating water cooling system 16; the fan 12 is disposed below the casing 1 so that cooling air blown by the fan can be directed toward the bottom of the casing.
In the present application, the circulating water cooling system 16 is disposed in the side wall and the bottom of the housing, respectively, that is, a cooling channel 161 is formed in the side wall and the bottom of the housing, and the cooling channel at the bottom of the housing flows through the bottom of each liquid storage tank to ensure the cooling effect; a liquid inlet 17 and a liquid outlet 18 are arranged outside the shell.
The liquid inlet is connected with a cooling liquid pump through a pipeline; the liquid outlet is connected with a radiator through a pipeline, and the radiator is connected with a cooling liquid pump through a pipeline.
The cooling liquid pump is in signal connection with the control unit. The cooling liquid pump is in signal connection with the control unit, when the control unit receives a shell internal temperature signal input by the temperature sensor, the control unit compares the shell internal temperature signal with a set temperature, if the input temperature is higher than the set temperature, the control unit outputs a control signal to the heating device to stop heating, meanwhile, the control unit outputs a control signal to the cooling liquid pump to control the cooling liquid pump to start the circulating water cooling system, and when an instant signal input by the temperature sensor is equal to or lower than the set temperature, the control unit outputs a stop control signal to control the cooling liquid pump to stop running.
In the above-mentioned circulating water cooling system, when the time set by the control unit reaches or the contact sensor between the casing and the casing cover inputs the opening signal of the casing cover, the control unit outputs a control signal to the cooling liquid pump to control the inspiration of the cooling liquid pump.
In the technical scheme of this application, be provided with the deep bead 20 of downwardly extending in three peripheries in the surface of casing bottom in the periphery, overall structure is that three deep bead 20 and casing bottom form a box-shaped structure that lacks a side, and the cooling air that the fan blew off is just to casing bottom surface.
The outer surface of the bottom of the shell is provided with a plurality of grooves 21, one end of each groove is communicated with one side of the bottom of the shell without the wind shielding part, and the central line of each groove is a curve.
The advantage of this technical scheme is, in current technical scheme, the fan is when moving, with cooling air direct blow to the casing bottom surface, in order to avoid this cooling air can disturb adjacent dyeing module, consequently, all be provided with the deep bead around the bottom of every casing, and such structure can lead to the cooling air that blows to the casing bottom and the cooling air that is heated that reflects from the casing bottom to run relatively, the result just so makes new cooling air blow to the bottom of casing and is hindered and increase the flow time, and simultaneously, the cooling air that is heated increases dwell time because of being hindered, and this condition just in time influences the cooling effect.
This technical scheme is through canceling one side in the deep bead all around with the bottom of casing to the surface in the bottom of casing sets up the central line and is curved recess, blows the cooling air of casing bottom like this and carries out the heat exchange back in the bottom with the casing, and the cooling air that the part is heated passes through the bottom of recess outflow casing, and the flow direction is different with the flow direction of the cooling air that the fan blows in addition, has avoided hindering, consequently the cooling wind speed that improves the cooling effect.
A wind shield 7 is arranged between two adjacent liquid storage tanks in the shell to prevent adjacent glass slides from being influenced when air is blown.
As shown in fig. 3, the axial centerline of the fan coincides with the housing bottom centerline; the cooling air blown out by the fan can be uniformly distributed to the bottom of the shell, so that the cooling uniformity of each liquid storage tank is ensured.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. A device for loading a sample dyeing module on an automatic dyeing machine is characterized by comprising a shell with a shell cover, wherein the shell cover is hinged with the shell through a connecting shaft; a plurality of liquid storage tanks are arranged in the shell;
the output shaft of the driving device transmits power to the connecting shaft so as to control the opening and closing of the shell cover;
a drainage tank is arranged in the shell and is communicated with each liquid storage tank; a drain hole is formed in the shell, one end of the drain hole is communicated with the drain groove, and the other end of the drain hole is opened on the outer side of the shell and is connected with a drain valve;
the heating device is arranged at the bottom of the shell;
the cooling device comprises a fan and a circulating water cooling system; the fan is arranged below the shell; the circulating water cooling system is respectively arranged on the side wall and the bottom of the shell;
wind shields extending downwards are arranged on three peripheries of the periphery of the bottom of the shell; at least more than two grooves are formed in the outer surface of the bottom of the shell, and one end of each groove is communicated with the periphery of the bottom of the shell, which is not provided with the wind shield; the central line of each groove is a curve;
and the control unit is respectively in signal connection with the driving device, the drain valve, the heating device and the fan.
2. The apparatus of claim 1, wherein a drainage plate and a waste liquid tank are arranged on the inner side of the housing cover, the drainage plate is arranged obliquely, and one side of the drainage plate close to the waste liquid tank is lower;
the waste liquid groove is communicated with the outer surface of the shell cover.
3. The device for the sample dyeing module of the automatic slice dyeing machine according to claim 1, characterized in that the output shaft of the driving device and the connecting shaft are in any one of belt transmission, chain transmission, gear transmission or hydraulic transmission.
4. The apparatus of claim 3, wherein the driving device is a motor fixed to the housing.
5. The apparatus of claim 1, wherein a temperature sensor is disposed within the housing, the temperature sensor being in signal communication with the control unit.
6. The apparatus of claim 1, wherein the circulating water cooling system is disposed in the side wall and the bottom of the housing, respectively; a liquid inlet and a liquid outlet are arranged on the outer side of the shell.
7. The device for the sample dyeing module on the automatic slice dyeing machine according to the claim 6, characterized in that the liquid inlet is connected with a cooling liquid pump through a pipeline; the liquid outlet is connected with a radiator through a pipeline, and the radiator is connected with the cooling liquid pump through a pipeline;
the cooling liquid pump is in signal connection with the control unit.
8. An apparatus for a sample staining module on an automatic slide staining machine as claimed in claim 1 wherein the axial centerline of the fan coincides with the housing bottom centerline.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810210860.6A CN108414333B (en) | 2018-03-14 | 2018-03-14 | Device for sample dyeing module on automatic slice dyeing machine |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810210860.6A CN108414333B (en) | 2018-03-14 | 2018-03-14 | Device for sample dyeing module on automatic slice dyeing machine |
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| CN108414333A CN108414333A (en) | 2018-08-17 |
| CN108414333B true CN108414333B (en) | 2020-12-29 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201425564Y (en) * | 2009-06-04 | 2010-03-17 | 福建泰普生物科学有限公司 | Pressure-bearing temperature control water cooling heat induced antigen retrieval device |
| CN103490597A (en) * | 2013-09-27 | 2014-01-01 | 深圳市禾望电气有限公司 | Cooling system of converter |
| CN105571925A (en) * | 2016-03-09 | 2016-05-11 | 福州迈新生物技术开发有限公司 | Dyeing module applied to biological samples on glass slides and dyeing method thereof |
| CN205581772U (en) * | 2016-04-22 | 2016-09-14 | 华北理工大学 | Computer liquid cooling ware |
| CN105975032A (en) * | 2016-06-27 | 2016-09-28 | 惠州市拉维尼科技有限公司 | Desktop type laptop cooler |
-
2018
- 2018-03-14 CN CN201810210860.6A patent/CN108414333B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201425564Y (en) * | 2009-06-04 | 2010-03-17 | 福建泰普生物科学有限公司 | Pressure-bearing temperature control water cooling heat induced antigen retrieval device |
| CN103490597A (en) * | 2013-09-27 | 2014-01-01 | 深圳市禾望电气有限公司 | Cooling system of converter |
| CN105571925A (en) * | 2016-03-09 | 2016-05-11 | 福州迈新生物技术开发有限公司 | Dyeing module applied to biological samples on glass slides and dyeing method thereof |
| CN205581772U (en) * | 2016-04-22 | 2016-09-14 | 华北理工大学 | Computer liquid cooling ware |
| CN105975032A (en) * | 2016-06-27 | 2016-09-28 | 惠州市拉维尼科技有限公司 | Desktop type laptop cooler |
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