CN108414306A - A kind of preprocess method for studying nano particle bio-toxicity effect - Google Patents
A kind of preprocess method for studying nano particle bio-toxicity effect Download PDFInfo
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- CN108414306A CN108414306A CN201810076546.3A CN201810076546A CN108414306A CN 108414306 A CN108414306 A CN 108414306A CN 201810076546 A CN201810076546 A CN 201810076546A CN 108414306 A CN108414306 A CN 108414306A
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- nano particle
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- bottomed bottle
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
Abstract
The invention discloses a kind of preprocess methods for studying nano particle bio-toxicity effect, include the following steps：S1：Lecithin is dissolved in the mixed solution of chloroform and methanol, obtains mixed solution A；S2：Mixed solution A is introduced into round-bottomed bottle, round-bottomed bottle is fixed on revolving instrument, rotation steaming method is carried out after temperature control, until forming thin film；S3：Round-bottomed bottle is put into vacuum drying chamber to be dried；S4：Nano particle and bead are sequentially added into round-bottomed bottle, is gently shaken up, and until all films fall off from bottle wall, are uniformly mixed；Round-bottomed bottle is put back to again on revolving instrument and rotated, finally bottled.The present invention provides a kind of preprocess method for studying nano particle bio-toxicity effect, it can be by synthesizing liposome using this method, liposomal encapsulated nano particle largely reduces the toxicity of nano particle, its stability is also improved simultaneously, this method is simply easily operated.
Description
Technical field
The present invention relates to bio-toxicity studying technological domains, more particularly to one kind is for studying nano particle bio-toxicity effect
The preprocess method answered.
Background technology
Nanotechnology is a kind of skill of research structure size property of material and application in 1 nanometer to 100 nanometer ranges
Art.The research and application of current nanotechnology are mainly in material and preparation, microelectronics and computer technology, Health and Medical, boat
It and aviation, environment and the energy, biotechnology and agricultural product etc..The equipment weight that makes of nano material is lighter, hardness
Stronger, longer life expectancy, maintenance cost are lower, design is more convenient.Using nano material can also produce special properties material or
The material that nature is not present, produces biomaterial and biomimetic material.But what is discharged during research and development, production and use receives
Rice grain can enter in environment water by all means, and constantly be accumulated in environment water.Nanometer ruler is arrived since substance is small
Great variety occurs for its property when spending, and macroscopically nontoxic substance at the nanoscale may be to biological production toxigenicity.Therefore, it grinds
Study carefully substance it is small to nano-scale when bio-toxicity it is significant, bio-safety review number can be provided for the application of nanometer product
According to, avoid on the ecosystem and human health cause seriously endanger even irreversible influence.
Currently, the bio-toxicity effect of nano particle is research hotspot.Nano particle type is various, such as nanogold, nanometer
Silver, nano-titanium dioxide, nano-sized carbon etc..Aquatile is applied more in biological subject, and there are commonly Daphnia magna, chlorella, water
Earthworm etc..However, comparison research papers are found, poisonous effect result of the same nano particle to same biological subject
The abnormal conditions such as big, poor repeatability that there may be othernesses, even conclusion be conflicting, cause toxicity assessment method to lack unified
Property and comparativity, data be not used to bio-safety evaluation.Through analysis, cause it is above-mentioned exception the main reason for include：Nanometer
Before grain is added to water body, is eaten by biological subject, may be occurred to reunite by water environmental impact, dissolving, precipitation, conversion etc. it is anti-
It answers, causes the physicochemical property (such as particle size, number concentration) of nano particle that unknown change occurs, to can not accurately establish
The correspondence of nano particle physicochemical property and bio-toxicity effect；Nano particle is added to water body, eaten by biological subject before,
Water body environment where biological subject may be impacted, to influence the toxicity data to biological subject indirectly.Therefore exist
When carrying out correlative study, how effectively to avoid nano particle not by biological subject surrounding environment influence, while ensureing nanometer again
Grain does not impact biological subject ambient enviroment, is a problem to be solved.
Invention content
To solve the above-mentioned problems, the present invention provides a kind of pretreatments for studying nano particle bio-toxicity effect
Method.
The specific technical solution of the present invention is：
A kind of preprocess method for studying nano particle bio-toxicity effect, wherein include the following steps：
S1：Lecithin is dissolved in the mixed solution of chloroform and methanol, obtains mixed solution A；
S2：Mixed solution A is introduced into round-bottomed bottle, then round-bottomed bottle is fixed on revolving instrument, is revolved after temperature control
Turn steaming method, until forming thin film；
S3：Round-bottomed bottle is put into vacuum drying chamber to be dried；
S4：Nano particle and bead are sequentially added into round-bottomed bottle, is gently shaken up, until all films are de- from bottle wall
It falls, is uniformly mixed；Round-bottomed bottle is put back to again on revolving instrument and rotated, finally bottled.
The preprocess method for studying nano particle bio-toxicity effect, wherein rotated in the step S2
Temperature is 35-60 DEG C.
The preprocess method for studying nano particle bio-toxicity effect, wherein in the step S1 and S4
Lecithin is 100-200mg；The total volume of chloroform and methanol is 5-15mL；Nano particle is to be received using prepared by liquid phase reduction
Rice grain, institute's dosage are 10-30mL, and state is 40-70 DEG C.
The preprocess method for studying nano particle bio-toxicity effect, wherein rotated in the step S4
Temperature setting is 40-70 DEG C, and the revolving time is set as 40-80min.
Advantageous effect：The present invention provides a kind of preprocess method for studying nano particle bio-toxicity effect, uses
This method can be by synthesizing liposome, and liposomal encapsulated nano particle is realized the encapsulating of nano particle, reduced to a certain extent
Influence of the nano particle to biological subject ambient enviroment, while also effectively preventing biological subject ambient enviroment to nano particle
Influence.This method is simple, easily operated.
Specific implementation mode
With reference to embodiment, the invention will be further described.
Embodiment 1
150mg lecithin is dissolved in the mixed liquor of 5ml chloroforms and methanol (volume ratio 2:1)；
Above-mentioned mixed liquor is introduced into the round-bottomed bottle of 250ml, round-bottomed bottle is fixed on revolving instrument, temperature control exists
40 DEG C, until forming thin film；After revolving, round bottom bottle is put into vacuum drying chamber and is dried.After drying,
The nano particle of 55 DEG C of 15ml prepared using liquid phase reduction is added into round-bottomed bottle, bead is added, gently shakes up, directly
It falls off, and is uniformly mixed from bottle wall to all films；Then round-bottomed bottle is put back to revolving instrument again, 60min is rotated at 55 DEG C,
It finally bottles spare.
By the liposome nano particle prepared and not encapsulated nano particle by concentration gradient to Daphnia magna into
Row viral infection test, the activity for observing Daphnia magna inhibit situation and death condition.Calculate LC50 and EC50.Pass through comparative test result
Nano particle is obtained after liposomal encapsulated, toxicity is decreased obviously.
Embodiment 2
180mg lecithin is dissolved in the mixed liquor of 5ml chloroforms and methanol (volume ratio 2:1)；
Above-mentioned mixed liquor is introduced into the round-bottomed bottle of 250ml, round-bottomed bottle is fixed on revolving instrument, temperature control exists
40 DEG C, until forming thin film；After revolving, round bottom bottle is put into vacuum drying chamber and is dried.After drying,
The nano particle of 60 DEG C of 20ml prepared using liquid phase reduction is added into round-bottomed bottle, bead is added, gently shakes up, directly
It falls off, and is uniformly mixed from bottle wall to all films；Then round-bottomed bottle is put back to revolving instrument again, 75min is rotated at 60 DEG C,
It finally bottles spare.
By the liposome nano particle prepared and not encapsulated nano particle by concentration gradient to Daphnia magna into
Row viral infection test, the activity for observing Daphnia magna inhibit situation and death condition.Calculate LC50 and EC50.Pass through comparative test result
Nano particle is obtained after liposomal encapsulated, toxicity is decreased obviously.
The present invention provides a kind of for studying the preprocess method of nano particle bio-toxicity effect, uses this method can be with
By synthesizing liposome, liposomal encapsulated nano particle realizes the encapsulating of nano particle, reduces nano particle to a certain extent
Influence to biological subject ambient enviroment, while also effectively preventing influence of the biological subject ambient enviroment to nano particle.It should
Method is simple, easily operated.
Raw materials used in the present invention, equipment is unless otherwise noted the common raw material, equipment of this field；In the present invention
Method therefor is unless otherwise noted the conventional method of this field.
The above is only presently preferred embodiments of the present invention, is not imposed any restrictions to the present invention, every according to the present invention
Technical spirit still falls within the technology of the present invention side to any simple modification, change and equivalent transformation made by above example
The protection domain of case.
Claims (4)
1. a kind of preprocess method for studying nano particle bio-toxicity effect, which is characterized in that include the following steps：
S1：Lecithin is dissolved in the mixed solution of chloroform and methanol, obtains mixed solution A；
S2：Mixed solution A is introduced into round-bottomed bottle, then round-bottomed bottle is fixed on revolving instrument, rotation steaming is carried out after temperature control
Method, until forming thin film；
S3：Round-bottomed bottle is put into vacuum drying chamber to be dried；
S4：Nano particle and bead are sequentially added into round-bottomed bottle, are gently shaken up, until all films fall off from bottle wall,
It is uniformly mixed；Round-bottomed bottle is put back to again on revolving instrument and rotated, finally bottled.
2. the preprocess method according to claim 1 for studying nano particle bio-toxicity effect, which is characterized in that
It is 35-60 DEG C that temperature is rotated in the step S2.
3. the preprocess method according to claim 1 for studying nano particle bio-toxicity effect, which is characterized in that
The lecithin is 100-200mg；The total volume of chloroform and methanol is 5-15mL；The nano particle is using liquid phase reduction
The nano particle of preparation, institute's dosage are 10-30mL, and state is 40-70 DEG C.
4. the preprocess method according to claim 1 for studying nano particle bio-toxicity effect, which is characterized in that
It is 40-70 DEG C that temperature setting is rotated in the step S4, and the revolving time is set as 40-80min.
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| CN201810076546.3A CN108414306A (en) | 2018-01-26 | 2018-01-26 | A kind of preprocess method for studying nano particle bio-toxicity effect |
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| CN201810076546.3A CN108414306A (en) | 2018-01-26 | 2018-01-26 | A kind of preprocess method for studying nano particle bio-toxicity effect |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102188439A (en) * | 2011-03-18 | 2011-09-21 | 浙江大学 | Nanoparticle liposome for releasing different medicines in programmed manner and preparation and use thereof |
| CN103768600A (en) * | 2014-01-25 | 2014-05-07 | 郑州大学 | Magnetic thermosensitive liposome nano-gold comopound, preparation method and application |
| CN104188908A (en) * | 2014-08-25 | 2014-12-10 | 广东医学院 | Method for preparing astragalus Sanxian soup flexible nano-liposome |
| CN105326792A (en) * | 2015-11-17 | 2016-02-17 | 上海纳米技术及应用国家工程研究中心有限公司 | Preparation method of compound with aqueous-phase nanogold wrapped with targeted liposomes |
-
2018
- 2018-01-26 CN CN201810076546.3A patent/CN108414306A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102188439A (en) * | 2011-03-18 | 2011-09-21 | 浙江大学 | Nanoparticle liposome for releasing different medicines in programmed manner and preparation and use thereof |
| CN103768600A (en) * | 2014-01-25 | 2014-05-07 | 郑州大学 | Magnetic thermosensitive liposome nano-gold comopound, preparation method and application |
| CN104188908A (en) * | 2014-08-25 | 2014-12-10 | 广东医学院 | Method for preparing astragalus Sanxian soup flexible nano-liposome |
| CN105326792A (en) * | 2015-11-17 | 2016-02-17 | 上海纳米技术及应用国家工程研究中心有限公司 | Preparation method of compound with aqueous-phase nanogold wrapped with targeted liposomes |
Non-Patent Citations (2)
| Title |
|---|
| YANGYANG ZHAO等: "SERS-active liposome@Ag/Au nanocomposite for NIR light-driven drug release", 《COLLOIDS AND SURFACE B：BIOINTERFACES》 * |
| 张灵芝: "《脂质体制备及其在生物医学中的应用》", 30 April 1998, 北京医科大学 中国协和医科大学联合出版 * |
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Application publication date: 20180817 |