CN108403808A - A kind of preparation method of Fructus Rosae Normalis extract - Google Patents
A kind of preparation method of Fructus Rosae Normalis extract Download PDFInfo
- Publication number
- CN108403808A CN108403808A CN201810284615.XA CN201810284615A CN108403808A CN 108403808 A CN108403808 A CN 108403808A CN 201810284615 A CN201810284615 A CN 201810284615A CN 108403808 A CN108403808 A CN 108403808A
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- CN
- China
- Prior art keywords
- roxburgh rose
- extract
- rosa roxburghii
- roxburghii tratt
- added
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/738—Rosa (rose)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/31—Extraction of the material involving untreated material, e.g. fruit juice or sap obtained from fresh plants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
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Abstract
The present invention provides a kind of preparation method of Fructus Rosae Normalis extract, includes the following steps：Step 1：Crushing roxburgh rose leaf, which is added in water, to be extracted, and roxburgh rose leaf water extract and roxburgh rose leaf filter residue are filtered to obtain；Step 2：Roxburgh rose leaf filter residue is added in ethyl alcohol, extraction and separation obtain Rosa roxburghii Tratt leaf extract；Step 3：Sodium ascorbate and potassium metabisulfite is soluble in water, Single Roxburgh Rose Fruit is added, smashes obtained pulp, filters to obtain Blackthorn pear fruit-juice and Rosa roxburghii Tratt pomace；Step 4：Rosa roxburghii Tratt pomace is added in ethyl alcohol and is extracted, filters and Single Roxburgh Rose Fruit alcohol extract is made；Step 5：Roxburgh rose leaf water extract, Blackthorn pear fruit-juice and Single Roxburgh Rose Fruit alcohol extract are mixed and concentrated, concentrate is added in Rosa roxburghii Tratt leaf extract and is mixed, Fructus Rosae Normalis extract is made.Rosa roxburghii Tratt is prepared into extract by the preparation method of Fructus Rosae Normalis extract provided by the invention for the first time, remains the active ingredient of Rosa roxburghii Tratt to greatest extent, improves Rosa roxburghii Tratt added value, enriches Rosa roxburghii Tratt intensive processing product.
Description
Technical field
The present invention relates to the extraction field of Rosa roxburghii Tratt, more particularly to a kind of preparation method of Fructus Rosae Normalis extract.
Background technology
Rosa roxburghii Tratt category rose family wild plant is the distinctive wild resource in the Yunnan-Guizhou Plateau, with Guizhou for the main place of production.Rosa roxburghii Tratt complete stool
With high medical value and edible value, pharmacological action and the chemical composition that it contains are closely related, many to Rosa roxburghii Tratt medicine
The research of reason effect is using the fruit juice of Rosa roxburghii Tratt or with Blackthorn pear fruit-juice preparation as main component.
Studies have shown that Rosa roxburghii Tratt fresh fruit contains abundant vitamin, polyphenol, flavones, organic acid, polysaccharide, amino acid and micro-
The substances such as secondary element.The organic acid that Rosa roxburghii Tratt contains is mainly malic acid, lactic acid, tartaric acid, citric acid, oxalic acid and succinic acid；Contain
Some higher fatty acids are linoleic acid, leukotrienes, oleic acid, palmitic acid and stearic acid；Rosa roxburghii Tratt also contains superoxide dismutase.This
Outside, roxburgh rose leaf can effectively treat scabies, carbuncle, metal-inflicted wound etc. as a kind of medicinal material, be primarily due to its contain a large amount of sitosterol,
Physcion, daucosterol, tangerine acetoacetic ester.
However, the functional component abundant relative to Rosa roxburghii Tratt, the working research of Rosa roxburghii Tratt is but asymmetric therewith.Currently, Rosa roxburghii Tratt produces
Most of product still belong to preliminary working product, i.e., Blackthorn pear fruit-juice are made by squeezing Single Roxburgh Rose Fruit, or impregnate roxburgh rose leaf and Rosa roxburghii Tratt is made
Leaf vegetation water.The functional component that this simple preliminary working product itself retains is few, and most of functional component also remains in processing
In residue, significant wastage is caused.Thus, the functional component in residue how is efficiently extracted, and prepare using Rosa roxburghii Tratt as raw material
Extract product, it is also to be improved.
Invention content
In view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to provide a kind of preparation method of Fructus Rosae Normalis extract, solve
Intensive processing product is less in existing Rosa roxburghii Tratt converted products.The preparation method of the present invention remains the effective of Rosa roxburghii Tratt to greatest extent
Ingredient improves Rosa roxburghii Tratt added value.
The present invention provides a kind of preparation method of Fructus Rosae Normalis extract, includes the following steps：
Step 1：Roxburgh rose leaf is crushed, roxburgh rose leaf powder is made, is added in water, 2h-10h is extracted under room temperature, is then filtered,
Roxburgh rose leaf water extract and roxburgh rose leaf filter residue is made；
Step 2：Roxburgh rose leaf filter residue obtained in step 1 is added in ethyl alcohol, then extraction and separation, obtains roxburgh rose leaf extraction
Object；
Step 3：Sodium ascorbate and potassium metabisulfite is soluble in water, Single Roxburgh Rose Fruit is added, mixes and smashes obtained fruit
Slurry, is then filtered, and Blackthorn pear fruit-juice and Rosa roxburghii Tratt pomace is made；
Step 4：Rosa roxburghii Tratt pomace obtained in step 3 is added in ethyl alcohol, 2h-10h is extracted, then filters, collects filtrate,
Single Roxburgh Rose Fruit alcohol extract is made；
Step 5：It will be made in Blackthorn pear fruit-juice obtained and step 4 in roxburgh rose leaf water extract obtained, step 3 in step 1
The Single Roxburgh Rose Fruit alcohol extract mixing obtained, is then concentrated into 1.2g/cm3-1.6g/cm3, concentrate is made, concentrate is added to step 2
In mix in Rosa roxburghii Tratt leaf extract obtained, Fructus Rosae Normalis extract is made.
Further, the Fructus Rosae Normalis extract prepares raw material in parts by weight, includes at least：1000-8000 parts of thorns
Pears leaf；7000-80000 parts of water；100-1500 parts of sodium ascorbates；1-6 parts of potassium metabisulfites；1000-30000 parts of Rosa roxburghii Tratts
Fruit；
Wherein, the dosage of water is 2000-20000 parts in step 1；The dosage of water is 5000-60000 parts in step 3.
Further, the roxburgh rose leaf for preparing in raw material is dry roxburgh rose leaf.
Further, the water for preparing in raw material is drinking water.
Further, ethyl alcohol uses volumetric concentration for the ethyl alcohol of 55%-75% in step 2, and dosage is 1000-10000 parts.
Further, the instrument extracted in step 2 uses supercritical carbon dioxide extraction equipment.
Further, the supercritical carbon dioxide extraction equipment includes extraction kettle, the first separating still and the second separating still；
When extraction kettle temperature reaches 40 DEG C -55 DEG C, the first separating still temperature reaches 35 DEG C -45 DEG C, the second separating still temperature reach 25 DEG C -
40 DEG C, extraction kettle pressure is adjusted to 20MPa-50MPa, the first separating still pressure to 5MPa-25MPa, the second separating still pressure is extremely
3MPa-10MPa, supercritical carbon dioxide extraction equipment start the cycle over extraction, control CO2Flow is to 5L/h-20L/h, when extraction
Between be 5h-20h.
Further, ethyl alcohol uses volumetric concentration for the ethyl alcohol of 45%-80% in step 4, and dosage is 2000-40000 parts.
Further, step 5 uses concentrating appts for membrane separation plant.
Rosa roxburghii Tratt is prepared into extract by the preparation method of Fructus Rosae Normalis extract provided by the invention for the first time, have convenient for preserve,
Convenient transportation drinks the advantages that convenient；Carbon dioxide supercritical extraction technology is applied in Rosa roxburghii Tratt deep-processing process, to greatest extent
Remain the active ingredient of Rosa roxburghii Tratt；Rosa roxburghii Tratt raw material originates from the Yunnan-Guizhou Plateau, and green natural is pollution-free, and intensive processing product improves thorn
Pears added value；The nutritional ingredient of roxburgh rose leaf, Single Roxburgh Rose Fruit is extracted, Rosa roxburghii Tratt intensive processing product is enriched.Meanwhile the present invention will make
Roxburgh rose leaf water extract, Blackthorn pear fruit-juice obtained and the Single Roxburgh Rose Fruit alcohol extract obtained concentration obtained, and extract with roxburgh rose leaf obtained
Object is taken to mix, Fructus Rosae Normalis extract obtained has abundant nutritional ingredient.
Specific implementation mode
Below in conjunction with the embodiment of the present invention, technical scheme in the embodiment of the invention is clearly and completely described,
Obviously, described embodiments are some of the embodiments of the present invention, instead of all the embodiments.Based on the implementation in the present invention
Example, the every other embodiment that those of ordinary skill in the art are obtained without creative efforts belong to
The scope of protection of the invention.
In the description of the present invention, it should be noted that term " first ", " second " are used for description purposes only, and cannot
It is interpreted as indicating or implying relative importance.
The explanation of term and method is provided below preferably to describe the average expert that the present invention and guides the art
Personnel put into practice the present invention：
Dry roxburgh rose leaf：By the roxburgh rose leaf being dried, water content is less.
Roxburgh rose leaf powder：By crushing, the roxburgh rose leaf aggregate of discrete particle obtained.
Extraction：Process soluble matter in medicinal material being transferred in suitable solvent.
Roxburgh rose leaf water extract：Substance in will be water-soluble in roxburgh rose leaf is transferred in water, the liquid of acquisition.
HA121-50-06 type supercritical carbon dioxide extraction equipments：For carrying out extracting substances under high pressure and suitable temperature
(solid or liquid), extract and spe medium are made molten in the separator by the change (such as pressure, temperature) of appointed condition
Solution substance is parsed to achieve the purpose that separation.The equipment includes an extraction kettle and two separating stills.
Single Roxburgh Rose Fruit alcohol extract：The substance that ethyl alcohol is dissolved in Single Roxburgh Rose Fruit is transferred in ethyl alcohol, the liquid of acquisition.
Ethanol extraction method：Refer to the dissolubility using ethyl alcohol, carries out the side of separating-purifying to substance using ethyl alcohol as solvent
Method.
UF membrane：The process of separation, the purifying, concentration of the different component of feed liquid is realized using the Selective Separation of film.
In order to become apparent from the technique effect of the description present invention, the present invention provides three groups of contrast experiments so as to the technology of this field
Personnel are easier to understand the technique effect of the present invention.
Contrast experiment 1
Embodiment 1：
Dry roxburgh rose leaf 1kg is taken, is added in FW177 type high speed Universal pulverizers, roxburgh rose leaf is ground into roxburgh rose leaf powder；It will
Roxburgh rose leaf powder is added to 2kg drinking water, extracts 6h at normal temperatures and pressures, primary every 3h stirrings；Roxburgh rose leaf water logging is made in filtering
Extract and roxburgh rose leaf filter residue；The step has economy by the nutritional ingredient in flooding roxburgh rose leaf, using water as solvent
It is easy to get, the advantages of polarity is big, and soluble end is wide, high safety, and solvent that can be strong with ethyl alcohol, glycerine and other polarity mixes；
Using HA121-50-06 type supercritical carbon dioxide extraction equipments, which includes extraction kettle, the first separation
Kettle and the second separating still；Above-mentioned roxburgh rose leaf filter residue is added in extraction kettle, it is 65% that 1kg volumetric concentrations directly, which are added, toward extraction kettle
Ethyl alcohol；Start extraction equipment, heats extraction kettle and two separating stills；When extraction kettle temperature reaches 40 DEG C, first detaches kettle temperature
Degree reaches 40 DEG C, when the second separating still temperature reaches 40 DEG C, opens CO2Gas cylinder, extraction kettle are forced into 20MPa, the first separating still
It is forced into 15MPa, the second separating still is forced into 10MPa, starts the cycle over extraction, controls CO2When flow is 5L/h, each kettle can be kept
Pressure balance；Pressure release after extraction 12.5h, stops extraction, opens extraction kettle and separating still bottom valve, obtains Rosa roxburghii Tratt leaf extract；Second
Alcohol is as solvent, the nutritional ingredient for dissolving roxburgh rose leaf filter residue；Using carbon dioxide supercritical extraction technology, protect to greatest extent
The active ingredient of Rosa roxburghii Tratt is stayed；
It takes 800g sodium ascorbates and 6g potassium metabisulfites to be dissolved in 5kg drinking water, 1kg Single Roxburgh Rose Fruits is added, add after mixing
Into MKK-XM-307 type multifunctional water fruit vegetable crushers, it is broken into pulp, filters and Blackthorn pear fruit-juice and Rosa roxburghii Tratt pomace is made；It is anti-
Bad hematic acid sodium is used as antioxidant, nutrition fortifier and the color stabilizer of food；Potassium metabisulfite is used as the fungicide of food；
Above-mentioned Rosa roxburghii Tratt pomace is added to the ethyl alcohol that 2kg volumetric concentrations are 60%, 6h is extracted under normal temperature and pressure, is stirred every 3h
Once；Single Roxburgh Rose Fruit alcohol extract is made in filtering；Ethyl alcohol is as solvent, the nutritional ingredient for dissolving Rosa roxburghii Tratt pomace；This step uses
Ethanol extraction method；
Above-mentioned roxburgh rose leaf water extract, Blackthorn pear fruit-juice and Single Roxburgh Rose Fruit alcohol extract are merged, mixing is added to BONA-GM-19 types
In organic membrane separation plant, it is concentrated into 1.4g/cm3, taking-up concentrate, which is added in above-mentioned Rosa roxburghii Tratt leaf extract, to be mixed, and Rosa roxburghii Tratt is made
Extract.It is concentrated and separated by membrane separation plant, enables to loss of effective components few, especially suitable for heat-sensitive substance,
Such as separation and concentration of antibiotic medicine, fruit juice, enzyme, albumen；Keep original flavor；Typical physical separating process, no
With chemical reagent and additive, product is not contaminated；Substance separation can be carried out in molecular level, and there is universal filter material can not replace
Excellent performance；Treatment scale is changeable, can continuously can also gap carry out, it is simple for process, it is easy to operate, be easy to automatic
Change；Electrical energy drive is only needed, energy consumption is extremely low, and expense is about 1/8th or even one third of evaporation and concentration or freeze concentration.
Control group 1：
Dry roxburgh rose leaf 1kg is taken, roxburgh rose leaf is ground into roxburgh rose leaf powder with FW177 types high speed Universal pulverizer, is added to 2kg
Drinking water extracts 6h at normal temperatures and pressures, primary every 3h stirrings, and filtering obtains Rosa roxburghii Tratt water extract.By the ascorbic acid of 800g
Sodium and 6g potassium metabisulfites are dissolved in 5kg drinking water, are mixed with 1kg Single Roxburgh Rose Fruits, using MKK-XM-307 type multifunctional water fruits and vegetables
Dish crusher is broken into pulp, and filtering obtains Blackthorn pear fruit-juice.Roxburgh rose leaf water extract and Blackthorn pear fruit-juice are merged, BONA- is passed through
The organic membrane separator of GM-19 types, is concentrated into 1.4g/cm3, obtain control group extract.
Main nutrient composition measures：
Vitamin C uses 2,6- dichloropheno-lindophenol titration measurings；Total reducing sugar (soluble sugar) uses potassium ferricyanide titration
It measures；Tannin uses titration of potassium permanganate；The measurement of Rosa roxburghii Tratt flavones uses spectrophotometry；Superoxide dismutase
(SOD) activity inhibits the autoxidation method of pyrogallol to measure using SOD.It is measured by above method, Rosa roxburghii Tratt of the invention carries
Object main nutrient composition value is taken to be above control group.
1. main nutrient composition of table measures
Contrast experiment 2
Embodiment 2：
Dry roxburgh rose leaf 4kg is taken, is added in FW177 type high speed Universal pulverizers, roxburgh rose leaf is ground into roxburgh rose leaf powder；It will
Roxburgh rose leaf powder is added to 11kg drinking water, extracts 10h at normal temperatures and pressures, primary every 5h stirrings；Roxburgh rose leaf water is made in filtering
Leaching liquor and roxburgh rose leaf filter residue；
Using HA121-50-06 type supercritical carbon dioxide extraction equipments, which includes extraction kettle, the first separation
Kettle and the second separating still；Above-mentioned roxburgh rose leaf filter residue is added in extraction kettle, it is 75% that 5kg volumetric concentrations directly, which are added, toward extraction kettle
Ethyl alcohol；Start extraction equipment, heats extraction kettle and two separating stills；When extraction kettle temperature reaches 47 DEG C, first detaches kettle temperature
Degree reaches 45 DEG C, when the second separating still temperature reaches 25 DEG C, opens CO2Gas cylinder, extraction kettle are forced into 35MPa, the first separating still
It is forced into 25MPa, the second separating still is forced into 3MPa, starts the cycle over extraction, controls CO2When flow is 12.5L/h, it can keep each
Kettle pressure balance；Pressure release after extraction 20h, stops extraction, opens extraction kettle and separating still bottom valve, obtains Rosa roxburghii Tratt leaf extract；
It takes 1.5kg sodium ascorbates and 1g potassium metabisulfites to be dissolved in 33kg drinking water, 15kg Single Roxburgh Rose Fruits is added, after mixing
It is added in MKK-XM-307 type multifunctional water fruit vegetable crushers, is broken into pulp, filter and Blackthorn pear fruit-juice and Rosa roxburghii Tratt pomace is made；
Above-mentioned Rosa roxburghii Tratt pomace is added to the ethyl alcohol that 21kg volumetric concentrations are 80%, 10h is extracted under normal temperature and pressure, is stirred every 5h
It mixes primary；Single Roxburgh Rose Fruit alcohol extract is made in filtering；
Above-mentioned roxburgh rose leaf water extract, Blackthorn pear fruit-juice and Single Roxburgh Rose Fruit alcohol extract are merged, mixing is added to BONA-GM-19 types
In organic membrane separation plant, it is concentrated into 1.6g/cm3, taking-up concentrate, which is added in above-mentioned Rosa roxburghii Tratt leaf extract, to be mixed, and Rosa roxburghii Tratt is made
Extract.
Control group 2：
Dry roxburgh rose leaf 4kg is taken, roxburgh rose leaf is ground into roxburgh rose leaf powder with FW177 types high speed Universal pulverizer, is added to
11kg drinking water, extracts 10h at normal temperatures and pressures, primary every 5h stirrings, and filtering obtains Rosa roxburghii Tratt water extract.By the anti-of 1.5kg
Bad hematic acid sodium and 1g potassium metabisulfites are dissolved in 33kg drinking water, are mixed with 15kg Single Roxburgh Rose Fruits, using the more work(of MKK-XM-307 types
Energy fruit and vegetable crusher is broken into pulp, filters, obtains Blackthorn pear fruit-juice.Roxburgh rose leaf water extract and Blackthorn pear fruit-juice are merged, led to
The organic membrane separator of BONA-GM-19 types is crossed, 1.6g/cm is concentrated into3, obtain control group extract.
Main nutrient composition measures：
Vitamin C uses 2,6- dichloropheno-lindophenol titration measurings；Total reducing sugar (soluble sugar) uses potassium ferricyanide titration
It measures；Tannin uses titration of potassium permanganate；The measurement of Rosa roxburghii Tratt flavones uses spectrophotometry；Superoxide dismutase
(SOD) activity inhibits the autoxidation method of pyrogallol to measure using SOD.It is measured by above method, Rosa roxburghii Tratt of the invention carries
Object main nutrient composition value is taken to be above control group.
2. main nutrient composition of table measures
Contrast experiment 3
Embodiment 3：
Dry roxburgh rose leaf 8kg is taken, is added in FW177 type high speed Universal pulverizers, roxburgh rose leaf is ground into roxburgh rose leaf powder；It will
Roxburgh rose leaf powder is added to 20kg drinking water, extracts 2h at normal temperatures and pressures, primary every 1h stirrings；Roxburgh rose leaf water logging is made in filtering
Extract and roxburgh rose leaf filter residue；
Using HA121-50-06 type supercritical carbon dioxide extraction equipments, which includes extraction kettle, the first separation
Kettle and the second separating still；Above-mentioned roxburgh rose leaf filter residue is added in extraction kettle, 10kg volumetric concentrations directly, which are added, toward extraction kettle is
55% ethyl alcohol；Start extraction equipment, heats extraction kettle and two separating stills；When extraction kettle temperature reaches 55 DEG C, first detaches
Kettle temperature degree reaches 35 DEG C, when the second separating still temperature reaches 32 DEG C, opens CO2Gas cylinder, extraction kettle are forced into 50MPa, first point
It is forced into 5MPa from kettle, the second separating still is forced into 6MPa, starts the cycle over extraction, controls CO2When flow is 20L/h, it can keep
Each kettle pressure balance；Pressure release after extraction 5h, stops extraction, opens extraction kettle and separating still bottom valve, obtains Rosa roxburghii Tratt leaf extract；
It takes 100g sodium ascorbates and 3g potassium metabisulfites to be dissolved in 60kg drinking water, 30kg Single Roxburgh Rose Fruits is added, after mixing
It is added in MKK-XM-307 type multifunctional water fruit vegetable crushers, is broken into pulp, filter and Blackthorn pear fruit-juice and Rosa roxburghii Tratt pomace is made；
Above-mentioned Rosa roxburghii Tratt pomace is added to the ethyl alcohol that 40kg volumetric concentrations are 45%, 2h is extracted under normal temperature and pressure, is stirred every 1h
It mixes primary；Single Roxburgh Rose Fruit alcohol extract is made in filtering；
Above-mentioned roxburgh rose leaf water extract, Blackthorn pear fruit-juice and Single Roxburgh Rose Fruit alcohol extract are merged, mixing is added to BONA-GM-19 types
In organic membrane separation plant, it is concentrated into 1.2g/cm3, taking-up concentrate, which is added in above-mentioned Rosa roxburghii Tratt leaf extract, to be mixed, and Rosa roxburghii Tratt is made
Extract.
Control group 3：
Dry roxburgh rose leaf 8kg is taken, roxburgh rose leaf is ground into roxburgh rose leaf powder with FW177 types high speed Universal pulverizer, is added to
20kg drinking water, extracts 2h at normal temperatures and pressures, primary every 1h stirrings, and filtering obtains Rosa roxburghii Tratt water extract.By the anti-bad of 100g
Hematic acid sodium and 3g potassium metabisulfites are dissolved in 60kg drinking water, are mixed with 30kg Single Roxburgh Rose Fruits, multi-functional using MKK-XM-307 types
Fruit and vegetable crusher is broken into pulp, and filtering obtains Blackthorn pear fruit-juice.Roxburgh rose leaf water extract and Blackthorn pear fruit-juice are merged, passed through
The organic membrane separator of BONA-GM-19 types, is concentrated into 1.2g/cm3, obtain control group extract.
Main nutrient composition measures：
Vitamin C uses 2,6- dichloropheno-lindophenol titration measurings；Total reducing sugar (soluble sugar) uses potassium ferricyanide titration
It measures；Tannin uses titration of potassium permanganate；The measurement of Rosa roxburghii Tratt flavones uses spectrophotometry；Superoxide dismutase
(SOD) activity inhibits the autoxidation method of pyrogallol to measure using SOD.It is measured by above method, Rosa roxburghii Tratt of the invention carries
Object main nutrient composition value is taken to be above control group.
3. main nutrient composition of table measures
Finally it should be noted that：The above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent
Present invention has been described in detail with reference to the aforementioned embodiments for pipe, it will be understood by those of ordinary skill in the art that：Its according to
So can with technical scheme described in the above embodiments is modified, either to which part or all technical features into
Row equivalent replacement；And these modifications or replacements, various embodiments of the present invention technology that it does not separate the essence of the corresponding technical solution
The range of scheme.
Claims (9)
1. a kind of preparation method of Fructus Rosae Normalis extract, it is characterised in that：Include the following steps：
Step 1：Roxburgh rose leaf is crushed, roxburgh rose leaf powder is made, be added in water, 2h-10h is extracted under room temperature, is then filtered, is made
Roxburgh rose leaf water extract and roxburgh rose leaf filter residue；
Step 2：Roxburgh rose leaf filter residue obtained in step 1 is added in ethyl alcohol, then extraction and separation, obtains Rosa roxburghii Tratt leaf extract；
Step 3：Sodium ascorbate and potassium metabisulfite is soluble in water, Single Roxburgh Rose Fruit is added, mixes and smashes obtained pulp, so
After filter, Blackthorn pear fruit-juice and Rosa roxburghii Tratt pomace is made；
Step 4：Rosa roxburghii Tratt pomace obtained in step 3 is added in ethyl alcohol, 2h-10h is extracted, then filters, collects filtrate, is made
Single Roxburgh Rose Fruit alcohol extract；
Step 5：It will be obtained in Blackthorn pear fruit-juice obtained in roxburgh rose leaf water extract obtained, step 3 in step 1 and step 4
Single Roxburgh Rose Fruit alcohol extract mixes, and is then concentrated into 1.2g/cm3-1.6g/cm3, concentrate is made, concentrate is added in step 2 and is made
It is mixed in the Rosa roxburghii Tratt leaf extract obtained, Fructus Rosae Normalis extract is made.
2. the preparation method of Fructus Rosae Normalis extract according to claim 1, it is characterised in that：The preparation of the Fructus Rosae Normalis extract
Raw material in parts by weight, includes at least：1000-8000 parts of roxburgh rose leafs；7000-80000 parts of water；100-1500 parts of ascorbic acid
Sodium；1-6 parts of potassium metabisulfites；1000-30000 parts of Single Roxburgh Rose Fruits；
Wherein, the dosage of water is 2000-20000 parts in step 1；The dosage of water is 5000-60000 parts in step 3.
3. the preparation method of Fructus Rosae Normalis extract according to claim 2, it is characterised in that：It is described to prepare roxburgh rose leaf in raw material
For dry roxburgh rose leaf.
4. the preparation method of Fructus Rosae Normalis extract according to claim 2, it is characterised in that：The water for preparing in raw material is drink
Use water.
5. the preparation method of Fructus Rosae Normalis extract according to claim 2, it is characterised in that：Ethyl alcohol uses volume in step 2
The ethyl alcohol of a concentration of 55%-75%, dosage are 1000-10000 parts.
6. the preparation method of Fructus Rosae Normalis extract according to claim 1, it is characterised in that：The instrument extracted in step 2 is adopted
Use supercritical carbon dioxide extraction equipment.
7. the preparation method of Fructus Rosae Normalis extract according to claim 6, it is characterised in that：The supercritical carbon dioxide extraction
Taking equipment includes extraction kettle, the first separating still and the second separating still；When extraction kettle temperature reaches 40 DEG C -55 DEG C, the first separating still
Temperature reaches 35 DEG C -45 DEG C, and the second separating still temperature reaches 25 DEG C -40 DEG C, adjusts extraction kettle pressure to 20MPa-50MPa, the
One separating still pressure is to 5MPa-25MPa, and the second separating still pressure to 3MPa-10MPa, open by supercritical carbon dioxide extraction equipment
Beginning cycling extraction controls CO2Flow is to 5L/h-20L/h, extraction time 5h-20h.
8. the preparation method of Fructus Rosae Normalis extract according to claim 2, it is characterised in that：Ethyl alcohol uses volume in step 4
The ethyl alcohol of a concentration of 45%-80%, dosage are 2000-40000 parts.
9. the preparation method of Fructus Rosae Normalis extract according to claim 1, it is characterised in that：Step 5 use concentrating appts for
Membrane separation plant.
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| CN108354105A (en) * | 2018-02-07 | 2018-08-03 | 厦门特旭生物科技有限公司 | A kind of Rosa roxburghii Tratt electuary and preparation method thereof |
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