CN108402277A - A kind of soy protein concentrate method of modifying - Google Patents
A kind of soy protein concentrate method of modifying Download PDFInfo
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- CN108402277A CN108402277A CN201810176094.6A CN201810176094A CN108402277A CN 108402277 A CN108402277 A CN 108402277A CN 201810176094 A CN201810176094 A CN 201810176094A CN 108402277 A CN108402277 A CN 108402277A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/346—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
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Abstract
The invention is related to a kind of soy protein concentrate method of modifying, including following step：Prepare Soy Protein Concentrate, prepare enzyme solutions, after Soy Protein Concentrate is pumped into microchannel pulverizer progress ultramicro grinding, it is pumped into the mixing module of micro passage reaction respectively with enzyme solutions again, hybrid reaction system enters reaction module and is reacted, then reaction system is made to flow through high temperature module passivation cellulase and proteinase activity, then the reaction system of acquisition is flowed through into refrigerated module, final reaction system flows out micro passage reaction, obtains the Soy Protein Concentrate being modified.Application of the soy protein concentrate prepared the invention further relates to preceding method in food and nutrition and health care field.The present invention has the advantages that reaction speed is fast, yield is high, superior product quality.
Description
Technical field
The invention belongs to soybean protein manufacture fields, and in particular to a kind of to be answered with microchannel crushing technology and microchannel plate
Device carries out enzymatic treatment soy protein concentrate to improve its dissolubility and improve its flavour characteristic method of modifying and its application.
Background technology
Soy protein concentrate is using low temperature soy meal as raw material, through the techniques such as extraction, precipitation, crushing, the non-egg of removal major part
A kind of obtained quality plant albumen of white soluble constituents.The protein content for the protein concentrate that alcohol lixiviation process produces
It is very high, it can reach 70% or so.But due to using a large amount of ethyl alcohol in process, sent out so as to cause protein molecular structure
Raw large change, nitrogen soluble index (NSI) value is relatively low, influences its application in the food industry.Outside isolating protein, soybean concentration
Albumen also contains the carbohydrate of 20% (butt) left and right, wherein insoluble polysaccharide can reach 10%~12% or so, greatly
The big solute effect for influencing product entirety.Grain size is 100 mesh or so to general merchandise soy protein concentrate after crushed, if directly
Connect it is edible, wherein the presence of insoluble polysaccharide largely effects on its flavor taste so that it is not suitable for making in the fields such as beverage
With.
In order to improve the dissolubility of alcohol processed soybean protein concentrate, scientific worker does a lot of work.For example, patent
CN102669407A is disclosed a kind of improving alcohol processed soybean protein concentrate using distributed fibers element enzyme and protease hydrolyzed technology
Deliquescent method；Patent CN102396642A is disclosed a kind of to be rolled over soy protein concentrate again under 280 degrees Celsius of environment
The method of folded 20min, the method to improve product NSI values；Patent CN105685367A discloses a kind of by adjusting pH value to 9
~11, it is spray-dried the method for obtaining modified soy protein concentrate product after then carrying out high-pressure homogeneous processing twice.
However, above-mentioned method of modifying cannot significantly reduce the particle of insoluble polysaccharide ingredient in soy protein concentrate
Diameter, in particular, insoluble granule grain size median is much larger than 20 microns.Patent CN201710044089.5 discloses a kind of new
Type microchannel crushing technology.Microchannel crushing technology is to utilize high pressure (being typically larger than 50MPa) by the fluid containing insoluble material
Quickly through a narrow microchannel grinder (channel internal diameter is 100~1000 microns) by unique design, fluid exists
Form complicated turbulent flow state in the grinder of microchannel, in fluid insoluble material particles hit mutually in field of turbulent flow,
A kind of new technique for rubbing and being crushed under special operating condition, can make insoluble object by this crushing technology
The grain size of material substantially reduces, so that can be less than 20 microns.
It has been had been reported that come insoluble polysaccharide ingredient in soy protein concentrate of degrading by zymolysis technique, however, public at present
The used zymolysis technique for opening report is carried out in batch tank reactor, and less efficient, the required time is longer, one
As need 2~20h.
Micro passage reaction is a kind of pipeline reactor continuously flowed, and wherein the internal diameter of the pipeline size general control exists
Between 10~1000 microns, chemistry or enzymic catalytic reaction are controlled the device in microresponse space.Micro passage reaction institute
The structure having determine wherein by fluid can greatly shorten time and the distance of transmission, while increasing specific surface
Product, may be implemented the quick mixing of material, to greatly improve mass-transfer efficiency.The narrow microchannel of micro passage reaction is simultaneously
It increases temperature and transmits gradient, increased specific surface area improves the heat-transfer capability of reactor significantly, compared with traditional heat exchangers extremely
It is few order of magnitude greater, thus greatly improve heat transfer efficiency.The unique texture of micro passage reaction and efficient mass-and heat-transfer effect
Rate so as to accurately controlling as possible for reaction process.Relative to traditional tank reactor gap reaction process, microchannel
Reactor can accurately adjust reaction process condition, such as be accurately controlled to reaction temperature, be accurately controlled to the reaction time
And material mixed proportion accurately controls, and can greatly improve reaction efficiency, reduces the generation of by-product, when shortening reaction
Between.The advantages of due to micro passage reaction, by conjunction with different supplemental functionalities can meet flexile chemical technology or
Person's enzymatic technological requirement.
Through studying for many years, soy protein concentrate is dispersed in water the present inventor by discovery, then microchannel is utilized to crush skill
Art can be reduced to soy protein concentrate insoluble solid grain diameter 20 microns of even smaller particles, in addition, utilizing high pressure
Soy protein concentrate aqueous systems after pump will crush are conveyed into micro passage reaction, using cellulase and protease,
Continuous enzymolysis is carried out in micro passage reaction, can greatly shorten enzymolysis time, usually less than 20min or even shorter time, so
The modified soybean concentrate egg white liquor that the above method is prepared is spray-dried afterwards, finally can obtain solubility property very
Excellent and insoluble solid grain diameter is less than 10 microns of modified soybean concentrated protein powder, can be widely used in such as
The food such as beverage and nutrition and health care field.
So far, there is not yet be used in combination microchannel crushing technology and micro passage reaction zymolysis technique concentrate it is big
Legumin modified technique is studied.The present invention provides one kind and microchannel crushing technology and micro passage reaction zymolysis technique is used in combination
Carry out soy protein concentrate modified technique method.
Invention content：
The object of the present invention is to provide one kind be used in combination microchannel crushing technology and micro passage reaction zymolysis technique into
The method that row soy protein concentrate is modified.The insoluble solid particle in soy protein concentrate can be effectively reduced through the invention
Grain size, and the dissolubility of alcohol method or the obtained soy protein concentrate of other methods can be promoted.
The present invention provides the following technical solution：A kind of soy protein concentrate method of modifying, including following step：
(1) Soy Protein Concentrate that mass concentration is 5~30% preferably 15~25% is prepared, it is 4.8 to adjust pH value
~8.0 preferably 5.5~7.5, it is sent in the grinder of microchannel and carries out ultramicro grinding；
(2) enzyme solutions are prepared：It is added into clear water and accounts for soy protein concentrate quality 0.5~2% preferably 1.0~1.75%
Cellulase and 0.5~2% preferably 1.0~1.75% neutral proteinase；
(3) Soy Protein Concentrate obtained after step (1) ultramicro grinding is pumped into the mixing of micro passage reaction
The enzyme solutions that step (2) has configured are pumped into the mixing module of micro passage reaction by module, realize Soy Protein Concentrate
With the mixing of enzyme solutions；
(4) the hybrid reaction system that step (3) obtains enters the reaction module of micro passage reaction and is reacted, and reacts
Temperature is 15~65 degrees Celsius, and preferably 45~50 degrees Celsius, reaction system residence time in reaction module is 30~1200 seconds,
It is preferred that 60~300 seconds；
(5) after the reaction for completing step (4), so that reaction system is flowed through high temperature module, reaction system heats up in the short time
To 75~95 degrees Celsius, the residence time is 10~1200 seconds, preferably 15~120 seconds, to be passivated cellulase and proteinase activity；
(6) reaction system that step (5) obtains is flowed through into refrigerated module, reaction system is cooled to 25~35 in the short time
Degree Celsius, it is 30~1200 seconds, preferably 30~300 seconds in the refrigerated module residence time；Final reaction system flows out microchannel plate
Device is answered, the Soy Protein Concentrate being modified is obtained.
Optionally, the good Soy Protein Concentrate of the modification that step (6) obtains is spray-dried, it is big obtains solid-state
Beans protein concentrate.
Further, Soy Protein Concentrate is sent by microchannel grinder, high-pressure pump using high-pressure pump in step (1)
30~120Mpa of pressure, preferably 60~80Mpa.
Further, Soy Protein Concentrate and enzyme solutions are pumped by micro- lead to using high-pressure delivery pump in step (3)
The mixing module of road reactor, 0.2~5MPa of high-pressure delivery pump pressure.
Further, in step (3), the temperature of mixing module is 0~45 degree Celsius, preferably 20~35 degree；Reaction system
The residence time is 30~1200 seconds, preferably 30~300 seconds in mixing module.
Further, in the step (5), the temperature of high temperature module is 75~105 degrees Celsius, and preferably 85~95 is Celsius
Degree.
Further, in the step (6), the temperature of refrigerated module is 0~30 degree Celsius, preferably 10~25 degree.
The present invention also provides the above method prepare soy protein concentrate food and nutrition and health care field application.
By the way that microchannel crushing technology and micro passage reaction technology is used in combination come modified soybean protein concentrate, has and pass
The incomparable advantage of system method of modifying, compared with prior art, the invention has the advantages that：
1. insoluble solid grain diameter in soy protein concentrate can be reduced effectively, mean particle size can be less than 20
Micron；The dissolubility for improving soy protein concentrate, up to 89%.Product good mouthfeel, without bitter taste and rough mouthfeel.With alcohol
It is compared for method soy protein concentrate, protein content 70%, dissolubility is poor, and contains 13% insoluble polysaccharide
Composition, insoluble granule grain size D50 are 300 microns.It is demonstrated experimentally that the solubility of non-modified alcohol method soy protein concentrate
For 15g/100g, i.e., 15%, after being modified to alcohol method soy protein concentrate using the present invention, solubility can reach
84%；
2. the efficiency of enzyme degradation reaction can be greatly improved, shorten the batch enzyme drop in reaction time, with tank reactor
Solution reaction is compared, and micro passage reaction is continous way enzyme degradation reaction, and same condition assigns the catalytic rate of micro passage reaction
95% or more can be reached and can save 70% or more time；
3. to reaction temperature and post-processing temperature can quickly accurately control, temperature when reaction can keep accurate
It is constant, after reaction, reaction system is warming up to certain temperature rapidly in very short time using high temperature module, quickly
It is passivated enzymatic activity.
Description of the drawings:
Fig. 1 is the process flow chart that the present invention prepares soy protein concentrate.
Fig. 2 is that soy protein concentrate passes through Universalpulverizer powder particle diameter distribution map.
Fig. 3 soy protein concentrate passes through microchannel pulverizer powder particle diameter distribution map.
Specific implementation mode
The most preferred embodiment of the present invention in a particular application is described in detail below, it should be noted that these realities
It is not to constrain the claims of the present invention to apply example.
Microchannel pulverizer used in the present invention is high-pressure homogeneous with reference to disclosed in patent CN201710044089.5
Machine is made, but is not limited to such microchannel pulverizer in actual use, can also select other similar
Microchannel disintegrating apparatus.
The fluid channel of each module of micro passage reaction used in the present invention has no unified regulation, length be 0.1m~
300m, preferably 1m~60m, the width and depth of fluid channel are 0.5 μm~10mm, preferably 0.1mm~0.5mm.Manufacture
The material of these modules can be stainless steel, extra hard steel, copper, ruby, diamond, ceramics, silicon carbide, cermet composite wood
Material or other high molecular materials.Micro passage reaction in the present invention can be the micro passage reaction of commercialization, for example, the U.S.
The heart-shaped structure G1 glass high throughput micro passage reactions of Corning Incorporated's production, or be prepared according to same principle other
Commercialization or non-commercial micro passage reaction.
High-pressure delivery pump in the present invention is preferentially using the high-pressure metering pump with function of measuring.
Embodiment 1：In the pulverizer of microchannel preparation soy protein concentrate is digested in ultramicro grinding and micro passage reaction
(1) device：
1) conventional Universalpulverizer
2) according to patent CN201710044089.5 embodiments 1, microchannel pulverizer is made, which includes
Microchannel grinder and high-pressure pump.
3) micro passage reaction device uses high pressure micro passage reaction, including mixing module, reaction module, high temperature module
And refrigerated module, micro-tunnel length determine that each module channels length used herein is according to flow velocity and reaction time
10m, caliber 0.3mm, heat transferring medium are conduction oil.
(2) preparation of enzymatic hydrolysis of soybean protein concentrate：
It is prepared by A- ultramicro grinding soy protein concentrate：
2kg soy protein concentrate (protein content 70%) is crushed with conventional Universalpulverizer (rotating speed 6000rpm)
The soy protein concentrate granular size D50 of 30min, obtained crushing are 97.7um, by the above-mentioned soybean concentrate eggs crushed of 2kg
8kg water is added in white particle (D90 165.34um), is being sufficiently stirred, and adjusts pH value=7, under the pressure of 80Mpa,
Entered by high-pressure pump and carry out ultramicro grinding in the grinder of microchannel, collects the soy protein concentrate slurry in exit to the first production
In product collecting tank (as shown in Figure 1), its grain diameter size is detected, as a result D90 is 7.01um.It then will be in product collecting tank
Protein concentrate solution is conveyed into the first material-compound tank.
The preparation of B- micro passage reactions：
Reaction system is controlled in the stop of modules by adjusting the flow of delivery pump and the passage length of microchannel
Between, wherein the residence time is 30 seconds in mixing module, and the residence time is 120 seconds in reaction module, the residence time in high temperature module
It it is 15 seconds, the residence time is 100 seconds in refrigerated module.
The temperature of reactor modules is controlled by outer circulation cooling and warming oil bath system, wherein mixing module
Temperature is 20 degrees Celsius, and the temperature of reaction module is 45 degrees Celsius, and the temperature of high temperature module is 90 degrees Celsius, the temperature of refrigerated module
Degree is 20 degrees Celsius.
C- complex enzymes prepare：
In the second material-compound tank, 20ml cellulases, 20ml neutral proteinases and 500ml pure water is added.
D- enzymic catalytic reactions：
High-pressure metering pump i.e. first metering pump of the connection with the first batch can and the second material-compound tank and the second metering pump are opened,
Under 25bar (i.e. 2.5MPa) pressure, high-pressure delivery so that Soy Protein Concentrate and composite enzyme solution enter microchannel plate
The mixing module of device is answered to be sufficiently mixed, the temperature of mixing module is 20 degrees Celsius, and it is anti-that mixed liquor subsequently enters 50 groups of series connection
Module is answered, the temperature of reaction module is 45 degrees Celsius, and reaction solution subsequently enters high temperature module, and high temperature module is 5 modules, high temperature
The temperature of module is 90 degrees Celsius, and reaction solution subsequently enters refrigerated module, and refrigerated module is 2 block coupled in series, refrigerated module
Temperature is 20 degrees Celsius, and then, reaction solution enters the second product collecting tank.
F- is spray-dried：
The liquid soybean protein concentrate digested in second product collecting tank is spray-dried, obtained product enters
In third product collecting tank, 1880g powder is finally obtained.
Product is through valuation officer sense organ taste, without bitter taste, without rough mouthfeel.Solubility can reach product in water
84%.
Embodiment 2：Reference examples corresponding with embodiment 1.
(1) device：
1) conventional Universalpulverizer.
2) 20L glass autoclave jacket reactor, mechanical agitation, heat transferring medium are conduction oil.
(2) preparation of enzymatic hydrolysis of soybean protein concentrate：
It is prepared by A- routine milling soya seeds protein concentrates：
2kg soy protein concentrate (protein content 70%) is crushed with conventional Universalpulverizer (rotating speed 6000rpm)
The soy protein concentrate granular size D90 of 30min, obtained crushing are 165.34um, and the above-mentioned soybean crushed of 2kg is concentrated
8kg water is added in protein body (D90 165.34um), is being sufficiently stirred, and adjusts pH value=7.
B- enzyme digestion reactions：
The above-mentioned soy protein concentrate slurry gathered is poured into 20L glass autoclave jacket reactors, starts mechanical agitation,
Speed of agitator is 70rpm, starts reaction kettle heating, and it is 45 degrees Celsius to keep temperature, and 20ml cellulases are added into reaction kettle
With 20ml neutral proteinases, reaction system is warming up to 90 degrees Celsius, the heating-up time by enzyme digestion reaction 3h immediately after reaction
It spends 30min, heat preservation 10min to be passivated enzyme activity, reaction system is then cooled to 20 degrees Celsius rapidly, temperature fall time is spent
45min。
C- is spray-dried：
The liquid soybean protein concentrate spray drying that enzymolysis is obtained, obtains 1750g powder.
Product has bitter taste through valuation officer sense organ taste, coarse mouthfeel.Solubility can reach 83% to product in water.
In embodiment 1, due to the use of microchannel crushing technology, thus the soy protein concentrate grain diameter being prepared is non-
Often tiny, the soy protein concentrate granular size D90 of obtained crushing is that 7.01um substantially improves flavor without rough mouthfeel
Taste quality.Enzyme digestion reaction is carried out due to the use of continuous flow micro passage reaction, greatly shortens the reaction time, especially in enzyme
Solution can be brought rapidly up passivation enzyme activity after reaction, can accurately control enzyme digestion reaction process, thus can be dense to avoid soybean
Pix protein transition enzymolysis generates bitter taste.
In this description, the present invention is described with reference to its specific embodiment.But it is clear that can still make
Various modifications and alterations without departing from the spirit and scope of the invention, still may be used by a those of ordinary skill for having this professional knowledge
With the technology taught according to this patent, other embodiments are generated within the scope of the invention, but it is every without departing from this hair
The content of bright technical solution, according to the technical essence of the invention to any simple modification, equivalent variations made by above example
With modification, in the range of still falling within technical solution of the present invention.
Claims (8)
1. a kind of soy protein concentrate method of modifying, which is characterized in that include following step：
(1) Soy Protein Concentrate that mass concentration is 5~30% preferably 15~25% is prepared, it is 4.8~8.0 to adjust pH value
It is preferred that 5.5~7.5, it is sent in the grinder of microchannel and carries out ultramicro grinding；
(2) enzyme solutions are prepared：The fiber for accounting for soy protein concentrate quality 0.5~2% preferably 1.0~1.75% is added into clear water
Plain enzyme and 0.5~2% preferably 1.0~1.75% neutral proteinase；
(3) Soy Protein Concentrate obtained after step (1) ultramicro grinding is pumped into the mixing module of micro passage reaction,
The enzyme solutions that step (2) has configured are pumped into the mixing module of micro passage reaction, realize Soy Protein Concentrate and enzyme
The mixing of solution；
(4) the hybrid reaction system that step (3) obtains enters the reaction module of micro passage reaction and is reacted, reaction temperature
It is 15~65 degrees Celsius, preferably 45~50 degrees Celsius, reaction system residence time in reaction module is 30~1200 seconds, preferably
60~300 seconds；
(5) after the reaction for completing step (4), so that reaction system is flowed through high temperature module, reaction system is warming up to 75 in the short time
~95 degrees Celsius, the residence time be 10~1200 seconds, preferably 15~120 seconds, to be passivated cellulase and proteinase activity；
(6) reaction system that step (5) obtains is flowed through into refrigerated module, it is Celsius that reaction system is cooled to 25~35 in the short time
Degree is 30~1200 seconds, preferably 30~300 seconds in the refrigerated module residence time；Final reaction system flows out micro passage reaction,
Obtain the Soy Protein Concentrate being modified.
2. soy protein concentrate method of modifying as described in claim 1, which is characterized in that the modification for obtaining step (6) is well
Soy Protein Concentrate be spray-dried, obtain solid-state soy protein concentrate.
3. soy protein concentrate method of modifying as claimed in claim 1 or 2, which is characterized in that use high-pressure pump in step (1)
Soy Protein Concentrate is sent into microchannel grinder, 30~120Mpa of high pressure pump pressure preferably 60~80Mpa.
4. soy protein concentrate method of modifying as claimed in claim 1 or 2, which is characterized in that defeated using high pressure in step (3)
The mixing module for sending pump that Soy Protein Concentrate and enzyme solutions are pumped into micro passage reaction, high-pressure delivery pump pressure 0.2
~5MPa.
5. method as claimed in claim 1 or 2, which is characterized in that in the step (3), the temperature of mixing module is 0~45
Degree Celsius, preferably 20~35 degree；Reaction system residence time in mixing module is 30~1200 seconds, preferably 30~300 seconds.
6. soy protein concentrate method of modifying as claimed in claim 1 or 2, which is characterized in that in the step (5), high temperature
The temperature of module is 75~105 degrees Celsius, preferably 85~95 degrees Celsius.
7. soy protein concentrate method of modifying as claimed in claim 1 or 2, which is characterized in that in the step (6), low temperature
The temperature of module is 0~30 degree Celsius, preferably 10~25 degree.
8. the soy protein concentrate prepared such as the method as described in claim 1-7 is any is in food and nutrition and health care field
Using.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711125493 | 2017-11-14 | ||
| CN2017111254931 | 2017-11-14 |
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| Publication Number | Publication Date |
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| CN108402277A true CN108402277A (en) | 2018-08-17 |
| CN108402277B CN108402277B (en) | 2021-11-26 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201810176094.6A Active CN108402277B (en) | 2017-11-14 | 2018-03-02 | Method for modifying concentrated soybean protein |
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| CN109169944A (en) * | 2018-10-30 | 2019-01-11 | 妙可蓝多（天津）食品科技有限公司 | A kind of low fat high calcium cheese and its production technology |
| CN110236064A (en) * | 2019-07-26 | 2019-09-17 | 深圳市绿航星际太空科技研究院 | A kind of instant full cereal oat solid beverage of high fine high protein and its processing technology |
| CN114376130A (en) * | 2020-10-22 | 2022-04-22 | 四川博明龙商务咨询有限公司 | Plant beverage and preparation method thereof |
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| CN109169944A (en) * | 2018-10-30 | 2019-01-11 | 妙可蓝多（天津）食品科技有限公司 | A kind of low fat high calcium cheese and its production technology |
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| CN114376130A (en) * | 2020-10-22 | 2022-04-22 | 四川博明龙商务咨询有限公司 | Plant beverage and preparation method thereof |
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| CN108402277B (en) | 2021-11-26 |
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