CN108387572A - Electrochemiluminescsystem system and electrochemical luminous detection method of the noble metal nanometer material as co-reactant - Google Patents
Electrochemiluminescsystem system and electrochemical luminous detection method of the noble metal nanometer material as co-reactant Download PDFInfo
- Publication number
- CN108387572A CN108387572A CN201810141013.9A CN201810141013A CN108387572A CN 108387572 A CN108387572 A CN 108387572A CN 201810141013 A CN201810141013 A CN 201810141013A CN 108387572 A CN108387572 A CN 108387572A
- Authority
- CN
- China
- Prior art keywords
- nanogold
- substance
- complex
- nano
- nano silver
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 12
- 239000000376 reactant Substances 0.000 title claims description 10
- 229910000510 noble metal Inorganic materials 0.000 title claims description 8
- 239000000463 material Substances 0.000 title claims description 7
- 239000000126 substance Substances 0.000 claims abstract description 43
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims abstract description 40
- 238000004020 luminiscence type Methods 0.000 claims abstract description 37
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 18
- 239000010970 precious metal Substances 0.000 claims abstract description 15
- 239000002245 particle Substances 0.000 claims abstract description 12
- 230000005281 excited state Effects 0.000 claims abstract description 11
- 230000005283 ground state Effects 0.000 claims abstract description 6
- 230000005855 radiation Effects 0.000 claims abstract description 6
- 238000003411 electrode reaction Methods 0.000 claims abstract description 5
- 239000010931 gold Substances 0.000 claims description 20
- 238000006243 chemical reaction Methods 0.000 claims description 17
- 229910052737 gold Inorganic materials 0.000 claims description 17
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 16
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 8
- 239000002086 nanomaterial Substances 0.000 claims description 6
- 229910052709 silver Inorganic materials 0.000 claims description 6
- 239000004332 silver Substances 0.000 claims description 6
- 239000003115 supporting electrolyte Substances 0.000 claims description 5
- 241000255789 Bombyx mori Species 0.000 claims description 4
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 4
- 239000002131 composite material Substances 0.000 claims description 4
- 235000010333 potassium nitrate Nutrition 0.000 claims description 4
- 239000004323 potassium nitrate Substances 0.000 claims description 4
- 239000010902 straw Substances 0.000 claims description 4
- 241000209094 Oryza Species 0.000 claims description 3
- 235000007164 Oryza sativa Nutrition 0.000 claims description 3
- YNPNZTXNASCQKK-UHFFFAOYSA-N Phenanthrene Natural products C1=CC=C2C3=CC=CC=C3C=CC2=C1 YNPNZTXNASCQKK-UHFFFAOYSA-N 0.000 claims description 3
- KJTLSVCANCCWHF-UHFFFAOYSA-N Ruthenium Chemical compound [Ru] KJTLSVCANCCWHF-UHFFFAOYSA-N 0.000 claims description 3
- DGEZNRSVGBDHLK-UHFFFAOYSA-N [1,10]phenanthroline Chemical compound C1=CN=C2C3=NC=CC=C3C=CC2=C1 DGEZNRSVGBDHLK-UHFFFAOYSA-N 0.000 claims description 3
- 230000005518 electrochemistry Effects 0.000 claims description 3
- 238000009396 hybridization Methods 0.000 claims description 3
- 230000028993 immune response Effects 0.000 claims description 3
- 229910052741 iridium Inorganic materials 0.000 claims description 3
- GKOZUEZYRPOHIO-UHFFFAOYSA-N iridium atom Chemical compound [Ir] GKOZUEZYRPOHIO-UHFFFAOYSA-N 0.000 claims description 3
- 239000002105 nanoparticle Substances 0.000 claims description 3
- 229910052762 osmium Inorganic materials 0.000 claims description 3
- SYQBFIAQOQZEGI-UHFFFAOYSA-N osmium atom Chemical compound [Os] SYQBFIAQOQZEGI-UHFFFAOYSA-N 0.000 claims description 3
- 235000009566 rice Nutrition 0.000 claims description 3
- 229910052707 ruthenium Inorganic materials 0.000 claims description 3
- 239000000084 colloidal system Substances 0.000 claims description 2
- 239000003446 ligand Substances 0.000 claims description 2
- 235000011164 potassium chloride Nutrition 0.000 claims description 2
- 239000001103 potassium chloride Substances 0.000 claims description 2
- KYQCOXFCLRTKLS-UHFFFAOYSA-N Pyrazine Chemical compound C1=CN=CC=N1 KYQCOXFCLRTKLS-UHFFFAOYSA-N 0.000 claims 2
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 claims 2
- PCNDJXKNXGMECE-UHFFFAOYSA-N Phenazine Natural products C1=CC=CC2=NC3=CC=CC=C3N=C21 PCNDJXKNXGMECE-UHFFFAOYSA-N 0.000 claims 1
- -1 gold Metal complex Chemical class 0.000 claims 1
- 235000012149 noodles Nutrition 0.000 claims 1
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 claims 1
- 238000005516 engineering process Methods 0.000 abstract description 7
- ROFVEXUMMXZLPA-UHFFFAOYSA-N Bipyridyl Chemical group N1=CC=CC=C1C1=CC=CC=N1 ROFVEXUMMXZLPA-UHFFFAOYSA-N 0.000 description 12
- 230000000694 effects Effects 0.000 description 10
- 238000000034 method Methods 0.000 description 8
- 239000012327 Ruthenium complex Substances 0.000 description 5
- DOIVPHUVGVJOMX-UHFFFAOYSA-N 1,10-phenanthroline;ruthenium Chemical compound [Ru].C1=CN=C2C3=NC=CC=C3C=CC2=C1.C1=CN=C2C3=NC=CC=C3C=CC2=C1.C1=CN=C2C3=NC=CC=C3C=CC2=C1 DOIVPHUVGVJOMX-UHFFFAOYSA-N 0.000 description 4
- 239000007853 buffer solution Substances 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 102000039446 nucleic acids Human genes 0.000 description 4
- 108020004707 nucleic acids Proteins 0.000 description 4
- 150000007523 nucleic acids Chemical class 0.000 description 4
- YFTHZRPMJXBUME-UHFFFAOYSA-N tripropylamine Chemical compound CCCN(CCC)CCC YFTHZRPMJXBUME-UHFFFAOYSA-N 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 238000003018 immunoassay Methods 0.000 description 3
- 238000012742 biochemical analysis Methods 0.000 description 2
- 239000007795 chemical reaction product Substances 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 230000002500 effect on skin Effects 0.000 description 2
- 238000005868 electrolysis reaction Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 235000021393 food security Nutrition 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000027756 respiratory electron transport chain Effects 0.000 description 2
- 238000006276 transfer reaction Methods 0.000 description 2
- IWSZDQRGNFLMJS-UHFFFAOYSA-N 2-(dibutylamino)ethanol Chemical compound CCCCN(CCO)CCCC IWSZDQRGNFLMJS-UHFFFAOYSA-N 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000027455 binding Effects 0.000 description 1
- 238000009739 binding Methods 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 238000005660 chlorination reaction Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 150000004696 coordination complex Chemical class 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 239000011263 electroactive material Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- HWYHZTIRURJOHG-UHFFFAOYSA-N luminol Chemical compound O=C1NNC(=O)C2=C1C(N)=CC=C2 HWYHZTIRURJOHG-UHFFFAOYSA-N 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000002082 metal nanoparticle Substances 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002070 nanowire Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- RXNXLAHQOVLMIE-UHFFFAOYSA-N phenyl 10-methylacridin-10-ium-9-carboxylate Chemical compound C12=CC=CC=C2[N+](C)=C2C=CC=CC2=C1C(=O)OC1=CC=CC=C1 RXNXLAHQOVLMIE-UHFFFAOYSA-N 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 150000003216 pyrazines Chemical class 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000006479 redox reaction Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000005641 tunneling Effects 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
Abstract
The present invention provides a kind of electrochemiluminescsystem system, which is used as coreaction reagent using precious metals complex as electrochemical luminescence reagent, using nanogold, nano silver or the substance that is respectively marked by it, they while still tested substance.The present invention also provides a kind of electrochemical luminous detection methods, using above-mentioned electrochemiluminescsystem system, apply DC potential to electrode, precious metals complex is oxidized to high price complex on anode；Electrode reaction product-high price complex captures the electronics of nanogold or nano silver, generates excited state particle；Unstable excited state particle is back to rapidly ground state, while radiation energy；By the variation of the electrochemical luminescence intensity or electrochemical luminescence intensity of record, the quality or concentration of nanogold, nano silver or its mark substance are determined.The present invention uses nanogold, nano silver or the substance that is respectively marked by it as coreaction reagent for the first time, realizes with Electrochemiluminescence technology Accurate Determining nanogold, nano silver and its substance marked.
Description
Technical field
The invention belongs to Electrochemiluminescence technology fields, and in particular to a kind of noble metal nanometer material is as co-reactant
Electrochemiluminescsystem system and electrochemical luminous detection method.
Background technology
Electrochemical luminescence (Electrochemiluminescence, ECL) is that certain chemical substances pass through electrode reaction,
The unstable excited state particle completed the electronics transfer of higher-energy and generated is discharged when returning to ground state in the form of light radiation
The process of energy.Popular says, electrochemical luminescence is the certain electroactive materials generated in electrode surface using electrolysis tech, then
Pass through chemiluminescence caused by redox reaction.Electrochemical luminescence can be divided into two kinds in the way of electrolysis：It is a kind of to use friendship
Pulse voltage is flowed, the research of reaction mechanism is mainly used for；Another kind uses DC voltage, is mainly used for actual chemical analysis.
Electrochemiluminescence mainly uses latter approach.
In electrochemical luminescence reaction, excited state particle is certain chemical substances by electron transfer reaction generation, but
All there is not all chemical substance this electro-chemical activity, the only compound of some specific structures to have this property
Matter.Common electrochemical luminescence reagent has the cooperation of the noble metals such as ruthenium, iridium, the terpyridyl of osmium, three Phens, three pyrazines
Object, luminol, acridinium ester and their certain derivatives.
In the electrochemical luminescence reaction of precious metals complex, it usually needs coreaction reagent (reducing agent) provides electronics
Electron transfer reaction is generated with excited state particle, common coreaction reagent mainly there are the chemical combination such as tripropyl amine (TPA), dibutylethanolamine
Object.So far without finding to use nanogold and nano silver as the report and patent of electrochemical luminescence co-reactant (reagent).
Nano material refer to substance three dimensions in at least one-dimensional space scale between 1 nanometer to 100 nanometers
Or the material being made of as unit it.They have unique chemical property and physical property, such as skin effect, micro-dimension effect
It answers, quantum effect and quantum tunneling effect etc., shows the superior function that conventional material does not have.Nanogold good in oxidation resistance,
It is most stable of noble metal nano particles.It not only has optical effect, skin effect, the small-size effect of general nano material
Outside quantum effect, also there is high electron density, dielectric property, catalytic action and extraordinary characterization of adsorption, it can be with a variety of lifes
Object macromolecular combines, and does not influence its bioactivity, and carrier and marker are often used as in biochemical analysis.
It is that protein and nucleic acid (DNA and RNA) are contour as the nano gold mark technology of one of modern four big labelling techniques
Molecule is adsorbed or is bonded to the process of nanometer gold surface.Since nanometer gold surface carries negative electrical charge, with the object with positive charge
Matter, such as immunoglobulin, toxin, glycoprotein, enzyme, antibiotic, hormone, DNA and RNA Non-covalent bindings, or with sulfydryl
Substance covalent bond, thus in immunoassay and foranalysis of nucleic acids become highly useful tool, food security, environment supervise
It surveys, the fields such as clinical detection are widely used.However, detection nanogold, the method for nano silver or the substance with its label
Colorimetric, luminosity, fluorescent technique or Electronic Speculum and microphotograph etc. are limited only to by means of visual method, it is difficult to which Accurate Determining is received
The amount of rice gold or nano silver, it is presently mainly colorimetric and by means of mesh especially to measure by the substance of nanogold or nano silver label
Depending on semi-quantitative method, accuracy is relatively low.In addition, so far without find using ElectrochemiluminescDetermination Determination nanogold, nano silver,
Or the report and patent of the substance of its label.
Invention content
The problem of can not accurately detecting nanogold, nano silver or the substance marked by it for the prior art, the present invention carries
For a kind of new electrochemical luminous detection method, the method is using the substance of nanogold, nano silver or its label as coreaction reagent
New electrochemiluminescsystem system is formed with precious metals complex, nanogold, nano silver are directly measured with Electrochemiluminescence
Or the substance of its label, so that nanogold and nano silver labelling technique is can be applied to Electrogenerated chemiluminescent immunoassay and nucleic acid point
Analysis.
The present invention is achieved by the following technical solutions：
A kind of electrochemiluminescsystem system of noble metal nanometer material as co-reactant, the system are made with precious metals complex
For electrochemical luminescence reagent, using nanogold, nano silver or its substance respectively marked as co-reactant.
Further, the precious metals complex is ruthenium complex, complex of iridium or osmium complex.
Further, the ligand of the precious metals complex be organic compound, preferably bipyridyl, Phen or they
Derivative.
Further, the nanogold or nano silver, at least the scale of the one-dimensional space is less than 50nm in three dimensions,
It is preferred that 2~30nm.
Further, the nanogold is that nano Au colloid, nanowires of gold, Jenner's popped rice, gold nano a small bundle of straw, etc. for silkworms to spin cocoons on and outer surface are
The composite nano materials of the nucleocapsid of gold.
Further, the nano silver is that nano-silver colloid, nano silver wire, silver nano flower-like, silver nanoparticle a small bundle of straw, etc. for silkworms to spin cocoons on and outer surface are
The composite nano materials of the nucleocapsid of silver.
A kind of electrochemical luminous detection method, using electrochemical light-emitting detector, using above-mentioned electrochemiluminescsystem system,
Coreaction reagent is also tested substance simultaneously, applies 0 to electrode-and the scanning potential of 1.5V makes metal complex aoxidize
DC potential, precious metals complex are oxidized to high price complex on anode；Electrode reaction product-high price complex is captured
The electronics of nanogold or nano silver generates excited state particle；Unstable excited state particle is back to rapidly ground state, while radiation energy
Amount；By the variation of the electrochemical luminescence intensity or electrochemical luminescence intensity of record, nanogold, nano silver or its label are determined
The quality or concentration of substance.
Further, it by measuring the quality or concentration of the substance marked by nanogold or nano silver, is sent out with labeled object
The amount of the substance of raw immune response or hybridization reaction is measured.
Further, the electrochemiluminescsystem system includes pH 6.0-and 8.0 buffer solution contains potassium nitrate or chlorination
Potassium supporting electrolyte.
Further, the precious metals complex in electrochemiluminescsystem system a concentration of 5.0 × 10–6─5.0×10–2Mol/L, preferably 5.0 × 10–4mol/L。
Compared with prior art, the invention has the advantages that：(1) tested substance is used for the first time-nanogold,
The substance of nano silver or its label forms new electrochemiluminescsystem system as coreaction reagent with precious metals complex；(2) real
The amount (quality or concentration) of the substance with Electrochemiluminescence technology Accurate Determining nanogold, nano silver or its label is showed；(3) lead to
The substance for directly measuring nanogold or nano silver label with Electrochemiluminescence technology is crossed, is provided for immunoassay and foranalysis of nucleic acids
Highly useful tool, is expected to be widely used in food security, environmental monitoring, in the fields such as clinical detection.
Description of the drawings
Fig. 1 is 1 electrochemical luminescence reaction principle schematic diagram of embodiment；
Fig. 2 is 1 nano Au particle concentration effect schematic diagram of embodiment；
Fig. 3 is 2 electrochemical luminescence reaction principle schematic diagram of embodiment；
Fig. 4 is the antibody concentration effect schematic diagram of 2 nano gold mark of embodiment.
Specific implementation mode
The synthesis of ruthenium complex is easier, price is relatively low, type is more, application technology is ripe, has been used as most common
Electrochemical luminescence reagent is applied in the biochemical analysis such as clinical examination, food inspection, environmental monitoring, below comparative example 1, embodiment
1 and 2 using ruthenium complex as luminescence reagent, with different coreaction reagents, chemical luminophor is described in detail and ties up to detection point
Application in analysis.
Comparative example 1
Using electrochemical workstation-Chemiluminescence Apparatus, using ruthenium complex as electrochemical luminescence reagent, use pH value for
6.0-8.0 buffer solution containing potassium nitrate supporting electrolyte applies DC potential, Ru (bpy) to electrode3 2+On anode
It is oxidized to Ru (bpy)3 3+, Ru (bpy) is reduced on cathode3 +, since they are generated on an electrode surface,
Therefore electrochemical luminescence reaction is not generated.In this case, if there are when reduced form co-reactant (coreaction reagent) in system
Following electrochemical luminescence reaction can then occur for (such as TPrA, tripropyl amine (TPA)).
By the electrochemical luminescence intensity of record, the amount of co-reactant (such as TPrA) can be measured, if deposited in the system
In the third party's substance for influencing reaction process, then by the variation of electrochemical luminescence intensity, third party's substance can be also measured.
Embodiment 1
Using electrochemical luminescence instrument (or electrochemical workstation-Chemiluminescence Apparatus), use pH value for 6.0-8.0 contain
The buffer solution of potassium nitrate supporting electrolyte, as shown in Figure 1, applying DC potential, ruthenium complex (Ru (bpy) to electrode3 2+)
Ru (bpy) is oxidized on anode3 3+, Ru (bpy) is reduced on cathode3 +, since they are generated in an electrode table
On face, therefore electrochemical luminescence reaction is not generated.In this case, if the sample there are nanogold or containing nanogold in system
When, electrode reaction product Ru (bpy)3 3+Capture the electronics of nanogold, generates excited state particle [Ru (bpy)3 2+] *, it is unstable
[Ru(bpy)3 2+] * is back to rapidly ground state Ru (bpy)3 2+, while radiation energy (luminous).
The electrochemical luminescence reaction principle indicated with chemical equation is as follows：
By the electrochemical luminescence intensity of record, the amount of nanogold or nano gold mark object can be measured, if the system
Middle to there is the third party's substance for influencing reaction process, then by the variation of electrochemical luminescence intensity, third party's substance can be also tested
It is fixed.
As shown in Fig. 2, nanogold has concentration effect in electrochemical luminescence reaction, i.e., when the amount of precious metals complex
(concentration or quality) timing, electrochemical luminescence intensity is directly proportional to quality (or the concentration) of gold nanoparticle, i.e., electrochemistry is sent out
Luminous intensity increases with the increase of nanogold quality (or concentration).
Embodiment 2
Using electrochemical luminescence instrument (or electrochemical workstation-Chemiluminescence Apparatus), use pH value for 6.0-8.0 contain
The buffer solution of potassium chloride supporting electrolyte, as shown in figure 3, applying DC potential to electrode, three adjacent Féraud beautiful jades close ruthenium (Ru
(phen)3 2+) it is oxidized to Ru (phen) on the electrode3 3+If there are when the antibody of nano gold mark (Au-Abx) in system, electricity
Pole reaction product Ru (bpy)3 3+Capture the electronics of nanogold, generates excited state particle [Ru (phen)3 2+] *, unstable [Ru
(phen)3 2+] * is back to rapidly ground state Ru (phen)3 2+, while radiation energy (luminous).
The electrochemical luminescence reaction principle indicated with chemical equation is as follows：
As shown in figure 4, the antibody of nano gold mark has concentration effect in electrochemical luminescence reaction, i.e., when noble metal is matched
The quality (or concentration) of amount (concentration or quality) timing for conjunction object, electrochemical luminescence intensity and nano gold mark antibody is at just
Than that is, electrochemical luminescence intensity increases with the increase of the quality (or concentration) of nano gold mark antibody.
By measure nanogold or nano silver label substance quality or concentration, with labeled object occur immune response or
The quality or concentration of the substance of hybridization reaction are measured.
Embodiment described above is only that the preferred embodiment of the present invention is described, not to the scope of the present invention
It is defined, under the premise of not departing from design spirit of the present invention, those of ordinary skill in the art are to technical scheme of the present invention
The various modifications made and improvement should all be fallen into the protection domain of claims of the present invention determination.
Claims (10)
1. a kind of electrochemiluminescsystem system of noble metal nanometer material as co-reactant, which is characterized in that the system is with your gold
Metal complex is as electrochemical luminescence reagent, using the substance of nanogold, nano silver or its label as co-reactant.
2. electrochemiluminescsystem system according to claim 1, which is characterized in that the precious metals complex coordinates for ruthenium
Object, complex of iridium or osmium complex.
3. electrochemiluminescsystem system according to claim 2, which is characterized in that the ligand of the precious metals complex is connection
Pyridine, Phen, connection pyrazine or their derivative.
4. electrochemiluminescsystem system according to claim 1, which is characterized in that the three-dimensional space of the nanogold or nano silver
Between at least the one-dimensional space size range be 2~30nm.
5. electrochemiluminescsystem system according to claim 1, which is characterized in that the nanogold is nano Au colloid, Jenner
Rice noodles, Jenner's popped rice, gold nano a small bundle of straw, etc. for silkworms to spin cocoons on and outer surface are the composite nano materials of the nucleocapsid of gold.
6. electrochemiluminescsystem system according to claim 1, which is characterized in that the nano silver is nano-silver colloid, nanometer
Nano silver wire, silver nano flower-like, silver nanoparticle a small bundle of straw, etc. for silkworms to spin cocoons on and outer surface are the composite nano materials of the nucleocapsid of silver.
7. electrochemiluminescsystem system according to claim 1, which is characterized in that the system includes pH 6.0-8.0 it is slow
Solution is rushed, which contains potassium nitrate or potassium chloride supporting electrolyte.
8. electrochemiluminescsystem system according to claim 1, which is characterized in that the precious metals complex is sent out in electrochemistry
A concentration of 5.0 × 10 in body of light system–6─5.0×10–2mol/L。
9. a kind of electrochemical luminous detection method, which is characterized in that electrochemical light-emitting detector is used, using claim 1
- 8 any one of them electrochemiluminescsystem systems, coreaction reagent are also tested substance simultaneously, apply direct current to electrode
Gesture, precious metals complex are oxidized to high price complex on anode；Electrode reaction product-high price complex captures nanogold
Or the electronics of nano silver, generate excited state particle；Unstable excited state particle is back to rapidly ground state, while radiation energy；It is logical
The variation of the electrochemical luminescence intensity or electrochemical luminescence intensity of overwriting determines nanogold, nano silver or its substance marked
Quality or concentration.
10. detection method according to claim 9, by measuring the quality of the substance of nanogold or nano silver label or dense
Degree occurs the quality of the substance of immune response or hybridization reaction with labeled object or concentration is measured.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810141013.9A CN108387572B (en) | 2018-02-11 | 2018-02-11 | Electrochemiluminescence system and electrochemiluminescence detection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810141013.9A CN108387572B (en) | 2018-02-11 | 2018-02-11 | Electrochemiluminescence system and electrochemiluminescence detection method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108387572A true CN108387572A (en) | 2018-08-10 |
| CN108387572B CN108387572B (en) | 2021-03-16 |
Family
ID=63068672
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810141013.9A Active CN108387572B (en) | 2018-02-11 | 2018-02-11 | Electrochemiluminescence system and electrochemiluminescence detection method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108387572B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114672862A (en) * | 2022-02-08 | 2022-06-28 | 中国科学技术大学 | Preparation method and application of viologen complex film |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102539411A (en) * | 2010-12-08 | 2012-07-04 | 郭良宏 | Method for enhancing ECL (electrochemical luminescence) signals by using nano-material electrodes |
| CN104614368A (en) * | 2014-12-02 | 2015-05-13 | 江南大学 | Fumonisin novel detection method based on electrogenerated chemiluminescence amplification system of gold-nanoparticle-supported iridium complex |
| CN104977293A (en) * | 2015-07-09 | 2015-10-14 | 东南大学 | Method for detecting metal ions through electrochemiluminescence |
| CN105241868A (en) * | 2015-09-18 | 2016-01-13 | 福建医科大学 | Electrochemiluminescence sensor based on methionine-gold nanocluster |
-
2018
- 2018-02-11 CN CN201810141013.9A patent/CN108387572B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102539411A (en) * | 2010-12-08 | 2012-07-04 | 郭良宏 | Method for enhancing ECL (electrochemical luminescence) signals by using nano-material electrodes |
| CN104614368A (en) * | 2014-12-02 | 2015-05-13 | 江南大学 | Fumonisin novel detection method based on electrogenerated chemiluminescence amplification system of gold-nanoparticle-supported iridium complex |
| CN104977293A (en) * | 2015-07-09 | 2015-10-14 | 东南大学 | Method for detecting metal ions through electrochemiluminescence |
| CN105241868A (en) * | 2015-09-18 | 2016-01-13 | 福建医科大学 | Electrochemiluminescence sensor based on methionine-gold nanocluster |
Non-Patent Citations (1)
| Title |
|---|
| 刘冬: ""金属纳米颗粒电极的电化学发光性能研究"", 《中国优秀硕士学位论文全文数据库 工程科技I辑》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114672862A (en) * | 2022-02-08 | 2022-06-28 | 中国科学技术大学 | Preparation method and application of viologen complex film |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108387572B (en) | 2021-03-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108287187B (en) | Electrochemical luminescence sensor | |
| JP4573486B2 (en) | Luminescence measurement method | |
| Valenti et al. | Transparent carbon nanotube network for efficient electrochemiluminescence devices | |
| Zhou et al. | Two orders-of-magnitude enhancement in the electrochemiluminescence of Ru (bpy) 32+ by vertically ordered silica mesochannels | |
| Dai et al. | An electrochemiluminescent sensor for methamphetamine hydrochloride based on multiwall carbon nanotube/ionic liquid composite electrode | |
| Zhang et al. | A new strategy for photoelectrochemical DNA biosensor using chemiluminescence reaction as light source | |
| Zhou et al. | Electrochemical synthesis of silver nanoclusters on electrochemiluminescent resonance energy transfer amplification platform for Apo-A1 detection | |
| Han et al. | Gold nanoparticles enhanced electrochemiluminescence of graphite-like carbon nitride for the detection of Nuclear Matrix Protein 22 | |
| CN106018390B (en) | The preparation of Ratio-type electrochemical luminescence paper chip and the application in lead ion detection | |
| Xue et al. | Electrochemiluminescence sensing platform based on functionalized poly-(styrene-co-maleicanhydride) nanocrystals and iron doped hydroxyapatite for CYFRA 21-1 immunoassay | |
| Saadati et al. | Immunosensing of breast cancer tumor protein CA 15-3 (carbohydrate antigen 15.3) using a novel nano-bioink: A new platform for screening of proteins in human biofluids by pen-on-paper technology | |
| CN112505120B (en) | Double-electrode photoelectrochemical immunosensor and preparation method thereof | |
| Luo et al. | Gold nanoclusters enhanced electrochemiluminescence of g-C3N4 for protein kinase activity analysis and inhibition | |
| CN102879582A (en) | In-vitro sandwich immunoassay process with magnetic separation for detecting alpha fetoprotein | |
| Kerr et al. | A comparison of commercially available screen-printed electrodes for electrogenerated chemiluminescence applications | |
| CN103472123A (en) | In-situ anode dissolving-out volt-ampere analytical method based on metal marking and biology affinity | |
| Han et al. | Detection of hydrogen peroxide with luminol electrogenerated chemiluminescence at mesoporous platinum electrode in neutral aqueous solution | |
| CN110554027A (en) | preparation method and application of immunosensor for promoting gold nanocluster electroluminescent response based on iron oxide array coreaction | |
| CN109100400B (en) | Sensor and its preparation method and application for detecting concanavalin A | |
| CN108802390A (en) | A kind of preparation of the pancreatic tumour marker immunosensor based on graphene-gold-palladium nanocomposite | |
| CN103048314A (en) | Electrochemical luminescence immune sensor built by mesoporous material loading quantum dots and coated by nanogold, and detection method of HIV (human immunodeficiency virus) | |
| CN103399058A (en) | High-sensitivity fullerene photoelectric chemical probe and preparation method thereof | |
| Liang et al. | A novel cloth-based multiway closed bipolar electrochemiluminescence biosensor for accurate detection of uric acid | |
| CN104865240B (en) | Disposable nanometer electrogenerated chemiluminescence two-component immunosensor and preparation method thereof | |
| CN108387572A (en) | Electrochemiluminescsystem system and electrochemical luminous detection method of the noble metal nanometer material as co-reactant |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |