CN108384881A - The fluorescent quantitative PCR detection method of coal dirt vacation tail spore - Google Patents
The fluorescent quantitative PCR detection method of coal dirt vacation tail spore Download PDFInfo
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- CN108384881A CN108384881A CN201810473961.2A CN201810473961A CN108384881A CN 108384881 A CN108384881 A CN 108384881A CN 201810473961 A CN201810473961 A CN 201810473961A CN 108384881 A CN108384881 A CN 108384881A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
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Abstract
Description
Number | Strain number | Latin name | Chinese | Source | Disease title |
1 | FQ10013003 | Pseudocercospora fuligena | The dirty false tail of coal Spore | Chinese Academy of Agricultural Sciences's vegetable or flower Research institute | Tomato coal dirt vacation tail spore leaf Pinta |
2 | HG15030802 | Corynespora cassiicola | Mostly main stick spore Bacterium | Chinese Academy of Agricultural Sciences's vegetable or flower Research institute | Tomato stick spore leaf spot |
3 | FQ130010701 | Botrytis cinerea | Botrytis cinerea Bacterium | Chinese Academy of Agricultural Sciences's vegetable or flower Research institute | Graw mold of tomato |
4 | HG15060516 | Fusarium oxysporum | Sharp spore reaping hook Bacterium | Chinese Academy of Agricultural Sciences's vegetable or flower Research institute | Tomato wilt |
5 | FQ14111912 | Stemphylium solani | Eggplant stemphylium | Chinese Academy of Agricultural Sciences's vegetable or flower Research institute | Tomato gray leaf spot |
6 | DBC16070604 | Rhizoctonia solani | Miliary damping-off Bacterium | Chinese Academy of Agricultural Sciences's vegetable or flower Research institute | Tomato seedling damping-off |
7 | HG13060501 | Fusarium solani | Eggplant disease reaping hook Bacterium | Chinese Academy of Agricultural Sciences's vegetable or flower Research institute | Tomato root rot |
8 | JGFQ1508070 2 | Alternaria solani | Alternaria solani sorauer Bacterium | Chinese Academy of Agricultural Sciences's vegetable or flower Research institute | Early blight of tomato |
9 | LJ08042301 | Sclerotinia sclerotiorum | Sclerotinite | Chinese Academy of Agricultural Sciences's vegetable or flower Research institute | Sclerotinia of tomato |
10 | LJ12010805 | Phytophthora capsici | Phytophthora capsici Bacterium | Chinese Academy of Agricultural Sciences's vegetable or flower Research institute | Tomato cotton disease |
Number | Latin name | Ct values | Copy number/μ L |
FQ16070301 | Pseudocercospora fuligena | 26.93 | 104.48 |
FQ16070302 | Pseudocercospora fuligena | 29.65 | 103.78 |
FQ16070303 | Pseudocercospora fuligena | 30.92 | 103.01 |
FQ16070304 | Pseudocercospora fuligena | 30.94 | 102.65 |
FQ16070305 | Pseudocercospora fuligena | 30.27 | 102.64 |
FQ16070306 | Pseudocercospora fuligena | 30.93 | 102.83 |
FQ16070307 | Pseudocercospora fuligena | 30.92 | 103.01 |
Claims (10)
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CN201810473961.2A CN108384881B (en) | 2018-05-17 | 2018-05-17 | Fluorescent quantitative PCR detection method of cercospora fuscogilva |
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CN201810473961.2A CN108384881B (en) | 2018-05-17 | 2018-05-17 | Fluorescent quantitative PCR detection method of cercospora fuscogilva |
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CN108384881A true CN108384881A (en) | 2018-08-10 |
CN108384881B CN108384881B (en) | 2021-08-17 |
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CN201810473961.2A Active CN108384881B (en) | 2018-05-17 | 2018-05-17 | Fluorescent quantitative PCR detection method of cercospora fuscogilva |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108977584A (en) * | 2018-09-05 | 2018-12-11 | 中国农业科学院蔬菜花卉研究所 | A kind of tomato mottle mosaic poison real-time fluorescence quantitative PCR detection method |
CN112725494A (en) * | 2020-12-31 | 2021-04-30 | 中国农业科学院蔬菜花卉研究所 | Method and primer for detecting 17 tomato pathogens by using micro-fluidic chip |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107119048A (en) * | 2017-05-15 | 2017-09-01 | 华南农业大学 | Mulberry Femoral pseudoaneurysm rDNA and its application in mulberry Femoral pseudoaneurysm Molecular Detection |
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2018
- 2018-05-17 CN CN201810473961.2A patent/CN108384881B/en active Active
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN107119048A (en) * | 2017-05-15 | 2017-09-01 | 华南农业大学 | Mulberry Femoral pseudoaneurysm rDNA and its application in mulberry Femoral pseudoaneurysm Molecular Detection |
Non-Patent Citations (2)
Title |
---|
MARC ZAHN等: "Quantification of Pseudocercospora fuligena in Tomato Lines Carrying Introgressions from Solanum habrochaites Using a qPCR Assay", 《PLANT DISEASE》 * |
李宝聚等: "李宝聚博士诊病手记(二十五) 由煤污假尾孢引起的疑似番茄叶霉病的病原鉴定", 《中国蔬菜》 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108977584A (en) * | 2018-09-05 | 2018-12-11 | 中国农业科学院蔬菜花卉研究所 | A kind of tomato mottle mosaic poison real-time fluorescence quantitative PCR detection method |
CN112725494A (en) * | 2020-12-31 | 2021-04-30 | 中国农业科学院蔬菜花卉研究所 | Method and primer for detecting 17 tomato pathogens by using micro-fluidic chip |
CN112725494B (en) * | 2020-12-31 | 2022-06-14 | 中国农业科学院蔬菜花卉研究所 | Method and primer for detecting 17 tomato pathogens by using micro-fluidic chip |
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