CN108373912A - A kind of salt-resisting high-temperature-resisting foam discharging agent and preparation method thereof - Google Patents
A kind of salt-resisting high-temperature-resisting foam discharging agent and preparation method thereof Download PDFInfo
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- C09K8/584—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids characterised by the use of specific surfactants
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Abstract
The present invention relates to a kind of salt-resisting high-temperature-resisting foam discharging agents and preparation method thereof, belong to oil-gas field development technical field.The salt-resisting high-temperature-resisting foam discharging agent of the present invention, consists of the following mass percentage components：Anionic and nonionic Surfactant Mixing Systems 0.2~0.5%, microbial fermentation solution 0.02~0.08%, surplus are water；The microbial fermentation solution is the microbial strains zymotic fluid for producing glycolipid biosurfactant or producing lipopeptide biosurfactant.The salt-resisting high-temperature-resisting foam discharging agent of the present invention, blistering, foam stabilizing with salt-resisting high-temperature-resisting, fluid-carrying capability, while there is good resistance to condensate, methanol tolerant performance, be conducive to the environmental protection and sustainable development of oil gas field.
Description
Technical field
The present invention relates to a kind of salt-resisting high-temperature-resisting foam discharging agents and preparation method thereof, belong to oil-gas field development technical field.
Background technology
The plurality of production liquid gas well in China has that liquid-gas ratio is high, drain difficulty, and to prevent gas well from giving birth to
At hydrate, Downhole Choking Technology is mostly used, production pressure reduction, drain difficulty is caused further to increase.
Using foams drain technique, can during gas-liquid two-phase mixes perpendicular flow, generate foam, dispersion, drag reduction,
A variety of physical-chemical effects such as washing reduce in pit shaft " slipping loss ", improve air-flow and vertically lift liquid energy power, reach raising row
The purpose of hydromining gas efficiency；And in fluid-discharge technology technology series, bubble row's construction technology is convenient, equipment is simple, it is easy for construction,
At low cost, applicable well depth range is big, does not influence gas well normally produces, Cheap highly effective, thus by common concern both domestic and external.
In general, can implement foaming water discharge successful, water pumping gas production efficiency is effectively improved, in very great Cheng
The performance of foam discharging agent used is depended on degree, therefore foam discharging agent is in optimized selection, proposes mark transformation, is bubble row's technical study hair
The most important thing.
For improving heat-resistant salt-resistant performance, methanol tolerance, the condensate oil performance of foam discharging agent, general thinking is preferred going out property
Can preferably foam discharging agent host agent (such as amphoteric surfactant), be aided with a variety of adjunct surfactants with synergistic function
(such as anion surfactant, nonionic surfactant, fluorocarbon surfactant), meanwhile, be equipped with some foaming aids and
Auxiliary agent (such as amine oxide, triethanolamine, ethylene glycol), come promoted whole system properties (such as 102212348 A of CN,
CN 105154053 A、CN 105199702A).From the point of view of live practical application effect, with natural surfactant-soapberry
Extract is as host agent, then is aided with foam discharging agent system prepared by a variety of surfactant single doses, is achieved preferably in some gas fields
Effect (such as 103,937,479 102660239 A of A, CN of CN), also, soapberry extract has certain beneficial environmental properties,
The feature of environmental protection of system can be improved to a certain extent.
With the fast development of state's economy, especially petrochemical industry there is a problem of it is extensive, inefficient, resource application it is fine
Change, greenization is increasingly becoming is the increasingly vital issue faced in nearest every profession and trade production activity.Biosurfactant is
When microorganism is cultivated under certain condition, the substance with surface-active secreted out of in metabolic process, as glycolipid, lipopeptid or
Neutral lipid derivative etc..Compared with chemically synthesized surfactant, biosurfactant, especially sugared Lipophylic surfaces are lived
Property agent molecular structure it is huge, complicated, except have efficiently reduce table/interfacial tension, can stable emulsion, increase foam, biology
Compatibility is good, it is nontoxic, can biodegradable, pollution-free, specificity and the advantages that good selectivity outside, certain sugared lipidic biomass tables
Face activating agent also has good antibacterial, bacteriostasis property, has both good chemical stability, resistance to highly basic, strong acid, this is generalization
The surfactant for learning synthesis is unapproachable.
It is a kind of based on biosurfactant that Authorization Notice No. is that the Chinese invention patent of CN 103555309B discloses
Water blocking damage treating agent, the water blocking damage treating agent include following components by mass percentage：Microbial fermentation solution 30~
70%, nonionic surface active agent 1~5%, fluorocarbon surfactant 0.5~1%, cationic surfactant 5~8%,
Surplus is distilled water, and wherein microbial fermentation solution is the zymotic fluid of pseudomonas aeruginosa；The water blocking damage of the patent disclosure is handled
Agent can effectively prevent and release water blocking damage, but its microbial fermentation solution and dosage of surfactant are larger, of high cost,
Easily affect to environment.
Authorization Notice No. is that the patent of invention of CN 103614131B discloses a kind of biochemical type salt-resisting high-temperature-resisting displacement of reservoir oil viscosity reduction
The displacement of reservoir oil thinner of agent and preparation method thereof, the patent disclosure includes following components by mass percentage：Microbial fermentation solution 30
~85%, anionic surfactant 0.5~5%, nonionic surface active agent 0.5~10%, surplus is distilled water；Institute
State the zymotic fluid that microbial fermentation solution is pseudomonas aeruginosa or bacillus subtilis；The displacement of reservoir oil thinner of the patent disclosure has
Higher high temperature resistant and resistance to salinity ability, but surfactant and microbial fermentation solution dosage are excessive, it is of high cost, and to ring
Border causes damages.
Invention content
The purpose of the present invention is to provide a kind of salt-resisting high-temperature-resisting foam discharging agent, which has good methanol tolerant, resistance to
Condensate performance.
Second object of the present invention is to provide a kind of preparation method of above-mentioned salt-resisting high-temperature-resisting foam discharging agent.
To achieve the above object, the technical scheme is that：
A kind of salt-resisting high-temperature-resisting foam discharging agent, consists of the following mass percentage components：Anion-nonionic surfactant is multiple
Zoarium system 0.2~0.5%, microbial fermentation solution 0.02~0.08%, surplus are water；The microbial fermentation solution is production glycolipid life
Object surfactant or the microbial strains zymotic fluid for producing lipopeptide biosurfactant.
The salt-resisting high-temperature-resisting foam discharging agent of the present invention can using anion-nonionic Surfactant Mixing Systems as foam discharging agent host agent
The microbial fermentation solution of efficient biosurfactant production is foaming aids, prepares a kind of biochemical compound heat and salinity tolerance bubble of environment-friendly type
Arrange agent；The content of microbial fermentation solution and anion-nonionic Surfactant Mixing Systems is extremely low in the salt-resisting high-temperature-resisting foam discharging agent,
But the frothing capacity and foam stability of foam discharging agent are on the one hand improved, on the other hand improves the environmental protection of foam discharging agent simultaneously
Property, and the water pumping gas production efficiency of water-producing gas well can be significantly improved, be conducive to high yield, the stable yields of high-liquid-ratio gas well, can meet
The operating mode of high temperature, with high salt, high condensate, high methanol content, it is adaptable.
The anion-nonionic Surfactant Mixing Systems are pressed by anion surfactant and nonionic surfactant
According to 30~70:30~70 mass ratio compounds.The present invention answers anion surfactant and nonionic surfactant
After matching, mixed micelle can be formed, non-ionic molecule is intercalated between ionic molecule so that the originally close head base of ionic molecule
Between electrical repulsion weaken, the charge density of micelle reduces, and cmc (critical micelle concentration) is reduced, when solution reaches critical glue
When beam concentration, the surface tension of solution minimizes value, surface-active higher, and anion surfactant is lived with non-ionic surface
Property agent compounding it can be promoted to achieve the desired purpose under lower concentration；In addition, under electrostatic interaction, surfactant
It is staggered in micella, forms stable mosaic texture, macroscopically showing salt resistant character.
The anion surfactant is in alkylbenzene sulfonate, alpha-alkene sulfonate, high fatty acid amide sulfonate
Any one or a few.
Above-mentioned alpha-alkene sulfonate is preferably alpha-olefin sodium sulfonate.
Abovementioned alkyl benzene sulfonate is preferably neopelex.
Above-mentioned high fatty acid amide sulfonate is preferably N, N- oleoyl methyl sodium taurocholates.
The nonionic surfactant be fatty alcohol polyoxyethylene ether, alkyl phenol polyoxyethylene ether, polyoxyethylate amide,
Any one or a few in Span.
Above-mentioned Span is preferably Tween-40.
Abovementioned alkyl phenol polyethenoxy ether is preferably nonylphenol polyoxyethylene ether TX-40.
Above-mentioned polyoxyethylate amide is preferably polyoxyethylate amide 6503.
The microbial fermentation solution is by production glycolipid biosurfactant or the microbial bacteria of lipopeptide biosurfactant
Strain is fermented.
The microbial strains of the production glycolipid biosurfactant are pseudomonas aeruginosa.The pseudomonas aeruginosa
Preserving number is CGMCC No.1.2464.
The microbial strains of the production lipopeptide biosurfactant are bacillus amyloliquefaciens.The solution starch gemma bar
The preserving number of bacterium is CGMCC No.11950.
The salt-resisting high-temperature-resisting foam discharging agent of the present invention, serves not only as surfactant system, when it enters stratum from pit shaft, also
It can play and change stratum wetability, to release/prevent the effect of water blocking damage, to improve the production efficiency of gas well.
For oil-containing gas well, when foam discharging agent provided by the invention enters stratum from pit shaft, microbial fermentation solution passes through gap
Water shifts after being combined with the oil droplet closed on, and microorganism adsorption is converted to organic solvent, surface in oil droplet boundary, by hydrocarbon molecules
Activating agent, acid, alcohol, gas etc., organic solvent, which is dissolved in oil, reduces its viscosity；Surfactant can reduce oil with water, oil with
The interfacial tension of rock improves oil displacement efficiency, changes wetability, improves oil phase fluid ability；Acid can reduce in oil and pore throat
Carbonate content corrodes quartz and carbonate surface to improve effective permeability；Gas is dissolved in oil, viscosity is reduced, by drawing
Play oil droplet expansion and play the physics displacement of reservoir oil, to improve the production efficiency of oil-containing gas well.
The foam discharging agent of the present invention contains anion-nonionic Surfactant Mixing Systems, in general, non-ionic surface active
Agent is influenced by its architectural characteristic, and temperature tolerance is limited；And anion surfactant is poor with respect to salt tolerance；Anionic surface is lived
Property agent and nonionic surfactant compound, system on the one hand can be made to obtain better heatproof, salt tolerant comprehensive performance, can be with
Obtain lower cmc and other application performance；Also contain production glycolipid class or lipopeptid class biological surface in foam discharging agent component of the present invention
The microbial fermentation solution of activating agent, generated surfactant is generally using rhamnose structure, polypeptide structure, carboxyl as hydrophilic group
Group, with the natural structure feature with anion+nonionic surfactant.Therefore, the life that microbial fermentation solution generates
Object surfactant can enhance the synergistic function and compatibility of anion and nonionic surfactant, keep system whole
The performances such as blistering, foam stabilizing further promoted.
The foam discharging agent of the present invention has stronger high temperature resistance, salt resistance and the biology that can efficiently blister with metabolizable generate
The zymotic fluid of the bacterial strain of surfactant is foaming aids, is compounded by itself and the moon-non-surface-active agent, with what is compounded in situ
Method is prepared for a kind of biochemical compound salt-resisting high-temperature-resisting foam discharging agent of environment-friendly type, good with stratum liquid compatibility, is not only suitable for
Situations such as single high temperature, high salinity, high condensate, high methanol content problem gas well water drainage-gas recovery technology, be also applied for simultaneous
Have high temperature, high salinity, the water drainage-gas recovery technology containing condensate, the high challenges gas well containing methanol content etc..
A kind of preparation method of above-mentioned salt-resisting high-temperature-resisting foam discharging agent, includes the following steps：
1) microbial strains for producing glycolipid biosurfactant or production lipopeptide biosurfactant are inoculated in culture
It on base, is cultivated 1~3 day in 30~42 DEG C, forms bacterium colony；
2) it by the colony inoculation obtained by step 1) in seed culture medium, in 35~42 DEG C of shaken cultivations 1~4 day, must plant
Sub- liquid；
3) by the seed liquor access fermentation medium obtained by step 2), in 30~42 DEG C of shaken cultivations 2~5 days, centrifugation
Microbial fermentation solution is obtained, the seed liquor is 1 with fermentation medium volume ratio：20~50；
4) by anion-nonionic Surfactant Mixing Systems, microbial fermentation solution and water mixing to get.
Culture medium in step 1) is solid medium, agar powder in the solid medium, peptone, yeast powder,
The mass ratio of NaCl is 15~20：8~12：3~5：8~12.The pH of the solid medium is 7~8.
Preferably, in above-mentioned solid medium agar powder a concentration of 15~20g/L, a concentration of 8~12g/ of peptone
L, a concentration of 3~5g/L of yeast powder, a concentration of 8~12g/L of NaCl.The pH of the solid medium is 7~8.
It cultivates 1~3 day in 30~42 DEG C in step 1), is preferably cultivated 1~2 day in 30~37 DEG C.
By the colony inoculation obtained by step 1) in seed culture medium in step 2), the bacterium colony is the single bacterium picked out
It falls.
A concentration of 8~the 15g/L, NH of glucose in the seed culture medium of step 2)4A concentration of 1~3g/L of Cl,
K2HPO4·12H2A concentration of 2~the 4g/L, KH of O2PO4A concentration of 0.1~1g/L, MgSO4·7H2A concentration of the 0.1 of O~
0.5g/L, FeSO4·7H2A concentration of 0.1~0.3g/L of O.The pH of seed culture medium in step 2) is 7~8.
In 35~42 DEG C of shaken cultivations 1~4 day in step 2), preferably with the speed of 150~200rpm at 30~37 DEG C
Rate shaken cultivation 1~2 day.
A concentration of 10~12g/L of glucose in the fermentation medium of step 3), a concentration of 2~3g/ of L-sodium
L, a concentration of 0.5~1g/L, K of yeast powder2HPO4A concentration of 3~4g/L, KH2PO4A concentration of 1.5~2g/L, FeSO4's
A concentration of 0.005~0.01g/L, MnSO4A concentration of 0.005~0.01g/L, MgSO4A concentration of 0.01~0.02g/L,
CaCl2A concentration of 0.01~0.02g/L.The pH of fermentation medium in step 3) is 7~8.
Step 3) was preferably shaken in 30~37 DEG C, with the rate of 100~180rpm in 30~42 DEG C of shaken cultivations 2~5 days
Swing culture 2~5 days.
The anion-nonionic Surfactant Mixing Systems are pressed by anion surfactant and nonionic surfactant
According to 30~70:30~70 mass ratio compounds.
The salt-resisting high-temperature-resisting foam discharging agent of the present invention has green, the characteristic of environmental protection, uses with anion-nonionic surface-active
Agent compound system is host agent, reduces critical micelle concentration, reduces solution surface tension, promotes to reach expected under lower concentration
Purpose adds the compounding of microbial fermentation solution, has obtained a kind of blistering with salt-resisting high-temperature-resisting, foam stabilizing, fluid-carrying capability, together
When the salt-resisting high-temperature-resisting foam discharging agent with good resistance to condensate, methanol tolerant performance, be conducive to the environmental protection of oil gas field and hold
Supervention exhibition.
The preparation method of the salt-resisting high-temperature-resisting foam discharging agent of the present invention, first prepares microbial fermentation solution, uses microbial fermentation
Microbial fermentation solution is mixed to obtain the final product with anion-nonionic Surfactant Mixing Systems, avoids one by the technique that liquid compounds in situ
As necessary zymotic fluid concentration, extraction, the process redissolved when applying biosurfactant, preparation method is simple, improves life
Efficiency is produced, operating cost is reduced.
Description of the drawings
Fig. 1 is the infrared test figure of Glycolipids Biosurfactantss in 1 P. aeruginosa fermented liquid of embodiment；
Fig. 2 is the infrared test figure of Lipopeptide Biosurfactants in bacillus amyloliquefaciens zymotic fluid in embodiment 5.
Specific implementation mode
Embodiments of the present invention are described further below in conjunction with the accompanying drawings.
Embodiment 1
The salt-resisting high-temperature-resisting foam discharging agent of the present embodiment, consists of the following mass percentage components：It lives on anion-nonionic surface
Property agent compound system 0.2%, produces the P. aeruginosa fermented liquid 0.02% of Glycolipids Biosurfactantss, surplus is water；
Wherein anion-nonionic Surfactant Mixing Systems are by anionic surfactant sodium dodecylbenzene sulfonate and non-ionic surface
Activating agent fatty alcohol polyoxyethylene ether AEO 9 is according to 30：70 mass ratio is mixed to get.
The preparation method of the salt-resisting high-temperature-resisting foam discharging agent of the present embodiment, includes the following steps：
1) pseudomonas aeruginosa (Pseudomonas aeruginosa) CGMCC No.1.2464 are inoculated in the training of LB solids
It supports on base, is cultivated 3 days in 35 DEG C, form bacterium colony；The LB solid mediums are：Agar powder 15g/L, peptone 12g/L, yeast
Powder 3g/L, NaCl 12g/L, moisturizing to 1L, pH 7；
2) selecting step 1) obtained by single bacterium colony be inoculated in seed culture medium, in 35 DEG C of shaken cultivations 4 days, obtain seed
Liquid, the seed culture medium are：Glucose 8g/L, NH4Cl 3g/L, K2HPO4·12H2O 2g/L, KH2PO41g/L,
MgSO4·7H2O 0.1g/L, FeSO4·7H2O 0.3g/L, moisturizing to 1L, pH 7；The rate of above-mentioned oscillation is 100rpm；
3) by the seed liquor access fermentation medium obtained by step 2), in 35 DEG C of shaken cultivations 5 days, microorganism is centrifuged to obtain
Zymotic fluid, wherein fermentation medium are：Glucose 10g/L, L-sodium 3g/L, yeast powder 0.5g/L, K2HPO44g/L,
KH2PO41.5g/L, FeSO40.01g/L, MnSO40.005g/L, MgSO40.02g/L, CaCl20.01g/L, moisturizing is extremely
1L, pH 8；The rate of above-mentioned oscillation is 100rpm；Seed liquor and the volume ratio of fermentation medium are 1:50；Gained verdigris is false single
The infrared test figure of Glycolipids Biosurfactantss is shown in Fig. 1 in born of the same parents' fermented liquid；
4) by the pseudomonas aeruginosa of anion-nonionic Surfactant Mixing Systems, production Glycolipids Biosurfactantss
Zymotic fluid according to said ratio and water mixing to obtain the final product.
Embodiment 2
The salt-resisting high-temperature-resisting foam discharging agent of the present embodiment, consists of the following mass percentage components：It lives on anion-nonionic surface
Property agent compound system 0.3%, produces the P. aeruginosa fermented liquid 0.04% of Glycolipids Biosurfactantss, surplus is water；
Wherein anion-nonionic Surfactant Mixing Systems are by anion surfactant alpha-olefin sodium sulfonate and non-ionic surface active
TX-40 is according to 50 for agent alkyl phenol polyoxyethylene ether：50 mass ratio is mixed to get.
The preparation method of the salt-resisting high-temperature-resisting foam discharging agent of the present embodiment, includes the following steps：
1) pseudomonas aeruginosa (Pseudomonas aeruginosa) CGMCC No.1.2464 are inoculated in the training of LB solids
It supports on base, cultivates 2d in 37 DEG C, form bacterium colony；The LB solid mediums are：Agar powder 20g/L, peptone 8g/L, yeast powder
5g/L, NaCl 8g/L, moisturizing to 1L, pH 8；
2) selecting step 1) gained single bacterium colony be inoculated in seed culture medium, in 42 DEG C of shaken cultivation 2d, obtain seed liquor, institute
Stating seed culture medium is：Glucose 15g/L, NH4Cl 1g/L, K2HPO4·12H2O 3g/L, KH2PO42g/L, MgSO4·7H2O
0.5g/L, FeSO4·7H2O 0.1g/L, moisturizing to 1L, pH 8；The rate of above-mentioned oscillation is 150rpm；
3) by the seed liquor access fermentation medium obtained by step 2), in 30 DEG C of shaken cultivation 5d, verdigris vacation is centrifuged to obtain
Unit cell fermented liquid, wherein fermentation medium are：Glucose 12g/L, L-sodium 2g/L, yeast powder 1g/L, K2HPO4 3g/
L, KH2PO42g/L, FeSO40.005g/L, MnSO40.01g/L, MgSO40.01g/L, CaCl20.02g/L, moisturizing is extremely
1L, pH 7；The rate of above-mentioned oscillation is 150rpm；Seed liquor and the volume ratio of fermentation medium are 1:20；
4) by the P. aeruginosa fermented liquid obtained by anion-nonionic Surfactant Mixing Systems, step 3) according to upper
State proportioning and water mixing to obtain the final product.
Embodiment 3
The salt-resisting high-temperature-resisting foam discharging agent of the present embodiment, consists of the following mass percentage components：It lives on anion-nonionic surface
Property agent compound system 0.4%, produces the P. aeruginosa fermented liquid 0.06% of Glycolipids Biosurfactantss, surplus is water；
Wherein anion-nonionic Surfactant Mixing Systems by anion surfactant N, N- oleoyl methyls sodium taurocholate and it is non-from
Sub- surfactant polyoxyethylate amide 6503 is according to 70：30 mass ratio is uniformly mixed so as to obtain with water.
The preparation method of the salt-resisting high-temperature-resisting foam discharging agent of the present embodiment, includes the following steps：
1) pseudomonas aeruginosa (Pseudomonas aeruginosa) CGMCC No.1.2464 are inoculated in the training of LB solids
It supports on base, 42 DEG C of culture 1d form bacterium colony；The LB solid mediums are：Agar powder 18g/L, peptone 10g/L, yeast powder
4g/L, NaCl 10g/L, moisturizing to 1L, pH 7；
2) selecting step 1) gained single bacterium colony be inoculated in seed culture medium, in 38 DEG C of shaken cultivation 3d, obtain seed liquor, institute
Stating seed culture medium is：Glucose 10g/L, NH4Cl 2g/L, K2HPO4·12H2O 4g/L, KH2PO40.5g/L, MgSO4·
7H2O 0.2g/L, FeSO4·7H2O 0.2g/L, moisturizing to 1L, pH 7；The rate of above-mentioned oscillation is 200rpm；
3) by the seed liquor access fermentation medium obtained by step 2), in 35 DEG C of shaken cultivation 5d, verdigris vacation is centrifuged to obtain
Unit cell fermented liquid, wherein fermentation medium are：Glucose 10g/L, L-sodium 3g/L, yeast powder 0.5g/L, K2HPO4
4g/L, KH2PO41.5g/L, FeSO40.01g/L, MnSO40.005g/L, MgSO40.02g/L, CaCl20.01g/L is mended
Water is to 1L, pH 8；The rate of above-mentioned oscillation is 100rpm；Seed liquor and the volume ratio of fermentation medium are 1:50；
4) by the P. aeruginosa fermented liquid obtained by anion-nonionic Surfactant Mixing Systems, step 3) according to upper
State proportioning and water mixing to obtain the final product.
Embodiment 4
The salt-resisting high-temperature-resisting foam discharging agent of the present embodiment, consists of the following mass percentage components：It lives on anion-nonionic surface
Property agent compound system 0.5%, produces the P. aeruginosa fermented liquid 0.08% of Glycolipids Biosurfactantss, surplus is water；
Wherein anion-nonionic Surfactant Mixing Systems are by anionic surfactant sodium dodecylbenzene sulfonate and non-ionic surface
Activating agent polyol fatty acid ester Tween-40 is according to 40：60 mass ratio is mixed to get.
The preparation method of the salt-resisting high-temperature-resisting foam discharging agent of the present embodiment, includes the following steps：
1) pseudomonas aeruginosa (Pseudomonas aeruginosa) CGMCC No.1.2464 are inoculated in the training of LB solids
It supports on base, cultivates 3d in 30 DEG C, form bacterium colony；The LB solid mediums are：Agar powder 15g/L, peptone 12g/L, yeast
Powder 3g/L, NaCl 12g/L, moisturizing to 1L, pH 7；
2) selecting step 1) gained single bacterium colony be inoculated in seed culture medium, in 35 DEG C of shaken cultivation 4d, obtain seed liquor, institute
Stating seed culture medium is：Glucose 8g/L, NH4Cl 3g/L, K2HPO4·12H2O 2g/L, KH2PO41g/L, MgSO4·7H2O
0.1g/L, FeSO4·7H2O 0.3g/L, moisturizing to 1L, pH 7；The rate of above-mentioned oscillation is 100rpm；
3) by the seed liquor access fermentation medium obtained by step 2), in 35 DEG C of shaken cultivation 5d, verdigris vacation is centrifuged to obtain
Unit cell fermented liquid, wherein fermentation medium are：Glucose 10g/L, L-sodium 3g/L, yeast powder 0.5g/L, K2HPO4
4g/L, KH2PO41.5g/L, FeSO40.01g/L, MnSO40.005g/L, MgSO40.02g/L, CaCl20.01g/L is mended
Water is to 1L, pH 8；The rate of above-mentioned oscillation is 100rpm；Seed liquor and the volume ratio of fermentation medium are 1:50；
4) by the P. aeruginosa fermented liquid obtained by anion-nonionic Surfactant Mixing Systems, step 3) according to upper
State proportioning and water mixing to obtain the final product.
Embodiment 5
The salt-resisting high-temperature-resisting foam discharging agent of the present embodiment, consists of the following mass percentage components：It lives on anion-nonionic surface
Property agent compound system 0.2%, produces the bacillus amyloliquefaciens zymotic fluid 0.02% of Lipopeptide Biosurfactants, surplus is
Water；Wherein anion-nonionic Surfactant Mixing Systems are by anion surfactant alpha-olefin sodium sulfonate and non-ionic surface
Activating agent fatty alcohol polyoxyethylene ether AEO 9 is according to 50：50 mass ratio is mixed to get.
The preparation method of the salt-resisting high-temperature-resisting foam discharging agent of the present embodiment, includes the following steps：
1) bacillus amyloliquefaciens (Bacillus amyloliquefaciens, CGMCC No.11950) are inoculated in LB
On solid medium, 35 DEG C of culture 3d form bacterium colony；The LB solid mediums are：Agar powder 15g/L, peptone 12g/L,
Yeast powder 3g/L, NaCl 12g/L, moisturizing to 1L, pH 7；
2) selecting step 1) gained single bacterium colony be inoculated in seed culture medium, 35 DEG C of shaken cultivation 4d obtain seed liquor, described
Seed culture medium is：Glucose 8g/L, NH4Cl 3g/L, K2HPO4·12H2O 2g/L, KH2PO41g/L, MgSO4·7H2O
0.1g/L, FeSO4·7H2O 0.3g/L, moisturizing to 1L, pH 7；The rate of above-mentioned oscillation is 200rpm；
3) by seed liquor access fermentation medium obtained by step 2), 37 DEG C of shaken cultivation 3d centrifuge to obtain solution starch gemma
Bacillus fermentation liquid, the fermentation medium are：Glucose 10g/L, L-sodium 3g/L, yeast powder 0.5g/L, K2HPO4 4g/
L, KH2PO41.5g/L, FeSO40.01g/L, MnSO40.005g/L, MgSO40.02g/L, CaCl20.01g/L, moisturizing is extremely
1L, pH 7；The rate of above-mentioned oscillation is 100rpm；Seed liquor and the volume ratio of fermentation medium are 1:50；Gained solution starch bud
The infrared test figure of Lipopeptide Biosurfactants is shown in Fig. 2 in spore bacillus fermentation liquid；
4) by the bacillus amyloliquefaciens zymotic fluid obtained by anion-nonionic Surfactant Mixing Systems, step 3) according to
Said ratio and water mixing to obtain the final product.
Embodiment 6
The salt-resisting high-temperature-resisting foam discharging agent of the present embodiment, consists of the following mass percentage components：It lives on anion-nonionic surface
Property agent compound system 0.3%, produces the bacillus amyloliquefaciens zymotic fluid 0.04% of Lipopeptide Biosurfactants, surplus is
Water；Wherein anion-nonionic Surfactant Mixing Systems by anion surfactant N, N- oleoyl methyls sodium taurocholate and
TX-40 is according to 60 for nonionic surfactant alkyl phenol polyoxyethylene ether：40 mass ratio is mixed to get.
The preparation method of the salt-resisting high-temperature-resisting foam discharging agent of the present embodiment, includes the following steps：
1) bacillus amyloliquefaciens (Bacillus amyloliquefaciens, CGMCC No.11950) are inoculated in LB
On solid medium, 42 DEG C are cultivated 1d, and the LB solid mediums are：Agar powder 20g/L, peptone 8g/L, yeast powder 5g/L,
NaCl 8g/L, moisturizing to 1L, pH 8；
2) selecting step 1) gained LB solid medium single bacterium colonies be inoculated in seed culture medium, 42 DEG C of shaken cultivation 2d,
Seed liquor is obtained, the seed culture medium is：Glucose 15g/L, NH4Cl 1g/L, K2HPO4·12H2O 4g/L, KH2PO4
0.1g/L, MgSO4·7H2O 0.5g/L, FeSO4·7H2O 0.1g/L, moisturizing to 1L, pH 8；The rate of above-mentioned oscillation is
180rpm；
3) by seed liquor access fermentation medium obtained by step 2), 42 DEG C of shaken cultivation 2d centrifuge to obtain solution starch gemma
Bacillus fermentation liquid, the fermentation medium are：Glucose 12g/L, L-sodium 2g/L, yeast powder 1g/L, K2HPO43g/L,
KH2PO42g/L, FeSO40.005g/L, MnSO40.01g/L, MgSO40.01g/L, CaCl20.02g/L, moisturizing to 1L,
PH is 8；The rate of above-mentioned oscillation is 180rpm；Seed liquor and the volume ratio of fermentation medium are 1:20；
4) by the bacillus amyloliquefaciens zymotic fluid obtained by anion-nonionic Surfactant Mixing Systems, step 3) according to
Said ratio and water mixing to obtain the final product.
Embodiment 7
The salt-resisting high-temperature-resisting foam discharging agent of the present embodiment, consists of the following mass percentage components：It lives on anion-nonionic surface
Property agent compound system 0.4%, produces the bacillus amyloliquefaciens zymotic fluid 0.06% of Lipopeptide Biosurfactants, surplus is
Water；Wherein anion-nonionic Surfactant Mixing Systems are by anionic surfactant sodium dodecylbenzene sulfonate and nonionic
Surfactant polyoxyethylene amide 6503 is according to 50：50 mass ratio is mixed to get.
The preparation method of the salt-resisting high-temperature-resisting foam discharging agent of the present embodiment, includes the following steps：
1) bacillus amyloliquefaciens (Bacillus amyloliquefaciens, CGMCC No.11950) are inoculated in LB
On solid medium, 42 DEG C of culture 1d form bacterium colony；The LB solid mediums are：Agar powder 20g/L, peptone 8g/L, ferment
Female powder 5g/L, NaCl 8g/L, moisturizing to 1L, pH 8；
2) selecting step 1) gained single bacterium colony be inoculated in seed culture medium, 42 DEG C of shaken cultivation 2d obtain seed liquor, described
Seed culture medium is：Glucose 15g/L, NH4Cl 1g/L, K2HPO4·12H2O 4g/L, KH2PO40.1g/L, MgSO4·7H2O
0.5g/L, FeSO4·7H2O 0.1g/L, moisturizing to 1L, pH 8；The rate of above-mentioned oscillation is 180rpm；
3) by seed liquor access fermentation medium obtained by step 2), 40 DEG C of shaken cultivation 2d centrifuge to obtain solution starch gemma
Bacillus fermentation liquid, the fermentation medium are：Glucose 11g/L, L-sodium 2g/L, yeast powder 1g/L, K2HPO43g/L,
KH2PO42g/L, FeSO40.005g/L, MnSO40.01g/L, MgSO40.01g/L, CaCl20.02g/L, moisturizing to 1L,
PH is 8；The rate of above-mentioned oscillation is 180rpm；Seed liquor and the volume ratio of fermentation medium are 1:30；
4) by the bacillus amyloliquefaciens zymotic fluid obtained by anion-nonionic Surfactant Mixing Systems, step 3) according to
Said ratio and water mixing to obtain the final product.
Embodiment 8
The salt-resisting high-temperature-resisting foam discharging agent of the present embodiment, consists of the following mass percentage components：It lives on anion-nonionic surface
Property agent compound system 0.5%, produces the bacillus amyloliquefaciens zymotic fluid 0.08% of Lipopeptide Biosurfactants, surplus is
Water；Wherein anion-nonionic Surfactant Mixing Systems are by anion surfactant alpha-olefin sodium sulfonate and non-ionic surface
Activating agent polyol fatty acid ester Tween-40 is according to 50：50 mass ratio is mixed to get.
The preparation method of the salt-resisting high-temperature-resisting foam discharging agent of the present embodiment, includes the following steps：
1) bacillus amyloliquefaciens (Bacillus amyloliquefaciens, CGMCC No.11950) are inoculated in LB
On solid medium, 42 DEG C are cultivated 1d, and the LB solid mediums are：Agar powder 20g/L, peptone 8g/L, yeast powder 5g/L,
NaCl 8g/L, moisturizing to 1L, pH 8；
2) single bacterium colony obtained by step 1) is inoculated in seed culture medium, 42 DEG C of shaken cultivation 1d, obtains seed liquor, described kind
Sub- culture medium is：Glucose 15g/L, NH4Cl 1g/L, K2HPO4·12H2O 4g/L, KH2PO40.1g/L, MgSO4·7H2O
0.5g/L, FeSO4·7H2O 0.1g/L, moisturizing to 1L, pH 8；The rate of above-mentioned oscillation is 180rpm；
3) by seed liquor access fermentation medium obtained by step 2), 42 DEG C of shaken cultivation 2d centrifuge to obtain solution starch gemma
Bacillus fermentation liquid, the fermentation medium are：Glucose 12g/L, L-sodium 2g/L, yeast powder 1g/L, K2HPO43g/L,
KH2PO42g/L, FeSO40.005g/L, MnSO40.01g/L, MgSO40.01g/L, CaCl20.02g/L, moisturizing to 1L,
PH is 8；The rate of above-mentioned oscillation is 180rpm；Seed liquor and the volume ratio of fermentation medium are 1:20；
4) by the bacillus amyloliquefaciens zymotic fluid obtained by anion-nonionic Surfactant Mixing Systems, step 3) according to
Said ratio and water mixing to obtain the final product.
Experimental example 1
It is blistered to the salt-resisting high-temperature-resisting foam discharging agent in embodiment 1-8, fluid-carrying capability test：
Using Roche foam meter, by professional standard SY/T 6465-2000《Foam drainage gas recovery foaming agent evaluation method》
In test method, the foam discharging agent of preparation frothing capacity in various concentration and at use temperature, foam stability, blistering are taken
Liquid energy power is tested, as a result as shown in table 1-2.
The blistering of foam discharging agent prepared by 1 embodiment 1-8 of table, (70 DEG C of fluid-carrying capability；Salinity 10 × 104mg/L)
The blistering of foam discharging agent prepared by 2 embodiment 1-8 of table, (100 DEG C of fluid-carrying capability；Salinity 10 × 104mg/L)
Experimental example 2
To the foam discharging agent condensate oil in embodiment 1-8, methanol tolerance performance test：
Methanol compatibility is tested：A concentration of the 1.0% of each foam discharging agent；Test temperature：70℃；Salinity 10 × 104Mg/L,
Testing time 12h, the results are shown in Table 3.
Condensate oil compatibility is tested：Each foam discharging agent a concentration of 1.0%；Temperature：70℃；Salinity 10 × 104Mg/L is surveyed
Time 12h is tried, test result is shown in Table 4.
Methanol+condensate compatibility experiment：Each foam discharging agent a concentration of 1.0%；Temperature：70℃；Salinity 10 × 104mg/
L, testing time 12h, test result are shown in Table 5.
Foam discharging agent methanol tolerance performance：70℃；Salinity 10 × 104Mg/L the results are shown in Table 6.
Foam discharging agent condensate oil performance, 70 DEG C；Salinity 10 × 104Mg/L the results are shown in Table 7.
Foam discharging agent in 3 embodiment 1-8 of the table foam discharging agent compatibility under different methanol contents
Foam discharging agent in 4 embodiment 1-8 of the table foam discharging agent compatibility under different condensation oil contents
Foam discharging agent in 5 embodiment 1-8 of the table foam discharging agent compatibility under different methanol, condensation oil content
By table 3-5 Experiment of Compatibility it is found that the foam discharging agent of the present invention has good compatibility with methanol, salinity water, without heavy
Shallow lake phenomenon occurs.
The methanol tolerance performance of foam discharging agent in 6 embodiment 1-8 of table
The condensate oil performance of foam discharging agent in 7 embodiment 1-8 of table
By methanol tolerance and condensate oil experimental result it is found that the salt-resisting high-temperature-resisting foam discharging agent of the present invention can meet methanol
The gas field of content≤40%, condensation oil content≤20% uses.
Claims (10)
1. a kind of salt-resisting high-temperature-resisting foam discharging agent, which is characterized in that consist of the following mass percentage components：Anion-nonionic table
Face activating agent compound system 0.2~0.5%, microbial fermentation solution 0.02~0.08%, surplus is water；The microbial fermentation solution
For production glycolipid biosurfactant or the microbial strains zymotic fluid of production lipopeptide biosurfactant.
2. salt-resisting high-temperature-resisting foam discharging agent according to claim 1, which is characterized in that the anion-nonionic surfactant
Compound system is by anion surfactant and nonionic surfactant according to 30~70:30~70 mass ratio compounding and
At.
3. salt-resisting high-temperature-resisting foam discharging agent according to claim 2, which is characterized in that the anion surfactant is alkane
Any one or a few in base benzene sulfonate, alpha-alkene sulfonate, high fatty acid amide sulfonate.
4. salt-resisting high-temperature-resisting foam discharging agent according to claim 2, which is characterized in that the nonionic surfactant is fat
In fat alcohol polyoxyethylene ether, alkyl phenol polyoxyethylene ether, polyoxyethylate amide, Span any one or it is several
Kind.
5. salt-resisting high-temperature-resisting foam discharging agent according to claim 1, which is characterized in that the production glycolipid biosurfactant
Microbial strains be pseudomonas aeruginosa.
6. salt-resisting high-temperature-resisting foam discharging agent according to claim 1, which is characterized in that the production lipopeptide biosurfactant
Microbial strains be bacillus amyloliquefaciens.
7. a kind of preparation method of salt-resisting high-temperature-resisting foam discharging agent as described in claim 1, which is characterized in that including following step
Suddenly：
1) microbial strains for producing glycolipid biosurfactant or production lipopeptide biosurfactant are inoculated on culture medium,
It is cultivated 1~3 day in 30~42 DEG C, forms bacterium colony；
2) by the colony inoculation obtained by step 1) in seed culture medium, in 35~42 DEG C of shaken cultivations 1~4 day, seed liquor is obtained；
3) by the seed liquor access fermentation medium obtained by step 2), in 30~42 DEG C of shaken cultivations 2~5 days, centrifuge micro-
Bio-fermented liquid, the seed liquor are 1 with fermentation medium volume ratio：20~50；
4) by anion-nonionic Surfactant Mixing Systems, microbial fermentation solution and water mixing to get.
8. the preparation method of salt-resisting high-temperature-resisting foam discharging agent according to claim 7, which is characterized in that the culture in step 1)
Base is solid medium, agar powder in the solid medium, peptone, yeast powder, NaCl mass ratio be 15~20：8~
12：3~5：8~12.
9. the preparation method of salt-resisting high-temperature-resisting foam discharging agent according to claim 7, which is characterized in that the seed of step 2) is trained
Support a concentration of 8~15g/L, NH of glucose in base4A concentration of 1~the 3g/L, K of Cl2HPO4·12H2A concentration of 2~4g/ of O
L, KH2PO4A concentration of 0.1~1g/L, MgSO4·7H2A concentration of 0.1~the 0.5g/L, FeSO of O4·7H2A concentration of the 0.1 of O
~0.3g/L.
10. the preparation method of salt-resisting high-temperature-resisting foam discharging agent according to claim 7, which is characterized in that the fermentation of step 3)
A concentration of 10~12g/L of glucose in culture medium, a concentration of 2~3g/L of L-sodium, yeast powder a concentration of 0.5~
1g/L, K2HPO4A concentration of 3~4g/L, KH2PO4A concentration of 1.5~2g/L, FeSO4A concentration of 0.005~0.01g/L,
MnSO4A concentration of 0.005~0.01g/L, MgSO4A concentration of 0.01~0.02g/L, CaCl2A concentration of 0.01~
0.02g/L。
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