CN108370830A - A kind of artificial culture method of black pine strain - Google Patents
A kind of artificial culture method of black pine strain Download PDFInfo
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- CN108370830A CN108370830A CN201611068010.4A CN201611068010A CN108370830A CN 108370830 A CN108370830 A CN 108370830A CN 201611068010 A CN201611068010 A CN 201611068010A CN 108370830 A CN108370830 A CN 108370830A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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Abstract
The invention discloses a kind of artificial culture method of black pine strain, agricultural technology fields, it is characterized in that being carried out by following step：One, it is sporinite that extraction black pine thalline entity, which grinds powdering,；Two, culture medium prepares：40~50 parts of dry cornstalk slag, 20~30 parts of wood dust, grass cuttings are 30~40 parts broken, 20~30 parts of dry clay, 15~20 parts of fine sand, 8~10 parts of cow dung, and be uniformly mixed and become culture base-material；Three, culture greenhouse prepares；Four, culture medium goes out miscellaneous bacteria；Five, culture base-material fills out pond and plantation；Six, the management during growth：The mainly growth of management culture medium top cucumber, builds cucumber holder, keeps the darkness of culture base-material moist；Seven, fruiting time：Black pine growth time was best with 35~40 days, and cucumber also just ties okra fruit at this time, and the black pine thalline of underground is dug out after picking cucumber.
Description
Technical field
The present invention relates to a kind of cultural methods of black pine strain, belong to agricultural technology field.
Background technology
Black pine (the entitled Tuber melanosporum of Latin, English are known as perigord truffle), also referred to as ferfas,
It is a kind of wild edible fungi being grown on underground, outer surface is uneven, and color and luster is between dark-brown and black, in small convex
Shape is played, throughout grey or the texture of light/dark balance and white.Because distributing special smell, just there are many artificial fascinated from ancient times.
Usually side by side, it is known as cuisines " three big kings " with top food materials caviar, Foie Gras etc. in Europe.The growth conditions of black pine
Harshness, yield is rare, and the minority for being as far as is known distributed mainly on Alps and the Himalaya is regional, therefore price
It is extremely expensive.Black pine, commonly used in top food materials its personal value be in Europe it is arranged side by side with the advanced cuisines such as caviar, Foie Gras,
It is known as cuisines " three big kings ".Since growth condition is harsh, annual worldwide production is very rare, therefore prices are rather stiff,
Comparable to gold, " Black Diamond " that is referred to as on dining table.This color is pitch-dark, is abound with the sphere of hard scale and shell, like diamond
Shape protrusion has mysterious life experience, more produces many mysteries and explanation.Not Stas is for the disciple Theo of Aristotle
Greece's period unique botanist, he thinks that truffle is along with autumn rain and thunder and lightning and the plant of life；B.C. 1st century wishes
Cured this section of doctor's Christian Dior Leech then thinks that truffle is a kind of taproot without stem and leaf；And Rome author Puli knob this more his
《Natural history》In think that heat, rainwater and thunder and lightning cause the sick elongated cocoon in soil so having grown extraordinary-looking truffle；The Renaissance
After Roman Empire's decline that epoch, the sperm of stag allow natural biology to be become pregnant and grown truffle under the action of heat ..., people
People shies away to it, it is all regarded as ominous incantation and accorded with by entire Europe, it is believed that it is the incarnation of demon.
The maturity period of black pine, usually best season was in December to secondary usually in the March in annual November to next year
Between the March in year.The people of acquisition truffle is called " truffle hunter ", and each truffle hunter has a handed down from the older generations of the family treasure map with it,
Record place, time and size that former generation once found truffle.The process for finding truffle is very interesting, and various countries hunter uses
Method be also not quite similar.In France, people's custom is sow as the competent assistant of harvest black pine.The smell of sow is extremely
It is sensitive, the truffle for being embedded in 25 centimetres of undergrounds to 30 centimetres of depths can be smelt in 6 meters of remote places.It is said that this is because truffle
The taste of the male hormone sent out with smell and boar is quite similar, so sow shows special preference to it.But sow has
The defect of gluttonous truffle insanely can ride out it to eat up if hunter does not stop in time when sow finds truffle.
Diamond modern scientific research data on dining table show that black pine contains abundant protein, 18 kinds of amino acid (packets
Include 8 kinds of essential amino acids that human body cannot synthesize), unsaturated fatty acid, multivitamin, zinc, manganese, iron, calcium, phosphorus, selenium etc. must
Need trace element and sphingolipid, cerebroside, ceramide, triterpene, androsterone, adenosine, truffle acid, sterol, truffle polysaccharide,
The a large amount of metabolite such as truffle polypeptide has high healthy nutritive value.Wherein androsterone have it is supporing yang, conditioning it is endocrine
Remarkable efficacy；Sphingolipid compound has obviously in terms of preventing senile dementia, atherosclerosis and antitumor cell toxicity
Activity；Polysaccharide, polypeptide, triterpene have strengthen immunity, anti-aging, fatigue-resisting function, can be used for health-care body-nourishing.
The taste of mushroom, garlic, wet mud, honey, corn, bedbug, rotten leaf etc. is all once used to describe truffle fragrance,
Also someone often says that they distribute Moschus, sperm and the sheet taste that do not wash year in year out.Even profession is heard fragrant teacher and is described in this way：Gasoline
It is mingled with some garlicky and rotten egg tastes among taste, the breath for heap fermentation of falling leaves in some certain woods wetlands, mixing
One yeast and honey flavour.What taste actually, everyone conclusion are different.
The mankind eat the history that black pine has more than 2000 years, wherein the most prevailing with France, Italy, Spain.
Europe fresh food black pine when, fresh black pine is first cut into very thin thin slice with special food slicer, then with butter, Italy
Cheese is sprinkling upon together in lasagne or Italian hollow powder, it is stated that taste is very good.The traditional eating method of France is with black
Truffle fried egg, is cut into small fourth block by fresh black pine and is put into egg and stir evenly, stand overnight make the taste of black pine fully into
Enter in egg liquid, then conventional decoct is fried.As famous dish, juice is done with beefsteak in France and Italian exclusive restaurant with black pine
In it is very famous.
The earliest record in relation to black pine comes from B.C. 350 years.Ancient Greece man Theophrastus was claimed at that time
It is the Jupiter god thunder and lightning impact soil creation on Mt. Olympus.Ancient Roman scholar Pliny thinks its unrooted, nothing
Stem, without leaf but can growth and development, be a mystery of nature.Until Christian era 19th-century, scientist just determines that black pine is one
Kind is grown on the rare fungi of underground.Black pine be grown in remote, thickly forested mountains in the trees such as pine, rubber under primary soil in, with tree
The wooden root symbiosis forms mycorhiza, February of the maturity period in November to the next year in annual winter.The price is very expensive for truffle,
Likened to eatable black diamond in Europe.
Modern scientific research finds, (including human body cannot close for the protein containing wide variety in truffle, 18 kinds of amino acid
At 8 kinds of essential amino acids), unsaturated fatty acid, multivitamin, zinc, manganese, iron, calcium, phosphorus, the essential trace elements such as selenium, with
And sphingolipid, cerebroside, ceramide, triterpene, androsterone, adenosine, truffle acid, sterol, truffle polysaccharide, truffle polypeptide etc. are a large amount of
Metabolite.Report is recorded according to the study, and mushroom polysaccharide, polypeptide, triterpene have strengthen immunity, anti-aging, antifatigue effect
Deng；Androsterone has the conditioning endocrine remarkable efficacy of men and women；Sphingolipid compound prevent senile dementia, atherosclerosis with
And having apparent activity etc. in terms of antitumor, anti-cell toxicity, the presence of these rare native compounds may constitute truffle
The material base of mystery effect.
China black pine place of production is concentrated mainly on Yunnan Province and Sichuan Province and its surrounding area, the wherein larger main product of yield
Area, which includes the Huidong County in Yunnan Province, to be managed, Gaoligong Shan Mountain, and (Panzhihua City is known as the " block of China at present on the ground such as Sichuan Province's Panzhihua
The township of bacterium ").Chinese black pine kind is complicated, according to《Literature Review of Tuber Species in China》It indicates, China's truffle kind up to tens
Kind, but practical only 5 kinds or so with higher edible value.For general black pine before no full maturity, epidermis is soft
And it is fine and smooth, cross section is creamy white or grey；After maturation, epidermis is hard and has intensive verruca, and cross section white is big
Reason stone lines is more apparent.The higher black pine bacterium fragrance of quality is full, a kind of more unique nasal receptor of people is given, according to state
The detection and analysis of inside and outside biologist, ripe black pine is containing up to ten several above aromatic series native compounds.At present
With the fast development of Chinese truffle industry, the truffle resource in Yunnan and Sichuan increasingly depleted, market business price also with
When the river rises the boat goes up.
Black pine is very fastidious to growing environment, can not as long as the pH-value of sunlight, moisture or soil varies slightly
Growth, is the delicious food that cannot uniquely carry out plantation in good order in the world.Truffle can be grown under one tree, and side has been seen
Come the same another one tree but cannot, so far people be also confused about why.It is different from the general mushroom such as mushroom, pine
The spore of dew is not propagated by wind, gnaws the animal of truffle by those to propagate.Truffle is mainly grown in rubber
, can not free living existence this is because truffle cannot carry out photosynthesis under tree, hazel, beech and lime, it is necessary to by with
Symbiosis between certain tree roots obtains nutrient.
Invention content
In order to be the edible strain that can manually cultivate by the culture of black pine bacterium, a kind of training of black pine bacterium of my invention
The black pine bacterium purity of the method for supporting, this cultural method culture is high, normal growth, and safety is good.
A kind of artificial culture method of black pine strain solve the problems, such as to adopt the technical scheme that by following step into
Row：One, strain prepares：Ripe black pine thalline is removed the peel, it is sporinite or sporinite that extraction thalline entity, which grinds powdering,
The conidia powder of aggregate；Two, culture medium prepares：40~50 parts of dry cornstalk slag, 20~30 parts of wood dust, grass cuttings broken 30~40
Part, 20~30 parts of dry clay, 15~20 parts of fine sand, 8~10 parts of cow dung, and be uniformly mixed as culture base-material；Three, culture is big
Canopy prepares：Greenhouse is cultivated, light transmittance should be shaded with the enhancing with sunlight, keep shade luminous intensity, culture is set in greenhouse
Pond, 0.5~0.6 meter of culture base-material depth in pond；Four, culture medium goes out miscellaneous bacteria：Culture base-material is heated to 70 DEG C~80 DEG C one point
Naturally temperature is reduced after clock to 20 DEG C~30 DEG C；Five, culture base-material fills out pond and plantation：Culture base-material after high-temperature sterilization is loaded
Enter culture pond, when loading depth 20cm~30cm is sprinkled into black pine conidia powder, and surface sprinkles Lasiosphaera fenzlii strain conidia powder, and spreading is close
Degree：1g~1.5g/m2, it is further continued for loading culture base-material 30cm~20cm, is slightly flattened with tablet, after top dibbling cucumber seeds
Clear water is sprayed to humidity；Six, the management during growth：The mainly growth of management culture medium top cucumber, builds cucumber
Holder keeps the darkness of culture base-material moist, keeps soil moisture content to be not less than 16%, suitably sprays water when dry；Keep greenhouse
25 DEG C~30 DEG C of interior temperature is as the temperature for urging the mushroom phase；Seven, fruiting time：Black pine growth time with 35~40 days be it is best,
Cucumber also just ties okra fruit at this time, and the black pine thalline of underground is dug out after picking cucumber.
A kind of advantageous effect of the artificial culture method of black pine strain is：A kind of artificial culture method of black pine strain
It is mixed with clay, fine sand using several plant clast, black pine growing environment in natural imitation, and is planted on culture base-material
Cucumber is planted, it can be that black pine thalline makees parasitism in the way of the intake of nutrition that cucumber, which enriches flourishing root system, be black pine
Symbiotic characteristic and dependency characteristic create conditions, successfully turn out the black pine edible thalline of precious deliciousness.
Specific implementation mode
A kind of artificial culture method of black pine strain is mainly pressed following step and is carried out：One, strain prepares：By maturation
Black pine thalline is removed the peel, and extraction thalline entity grinds the conidia powder that powdering is sporinite or sporinite aggregate；Two, culture medium
Prepare：40~50 parts of dry cornstalk slag, 20~30 parts of wood dust, grass cuttings are 30~40 parts broken, 20~30 parts of dry clay, fine sand 15
~20 parts, 8~10 parts of cow dung, and be uniformly mixed as culture base-material；Three, culture greenhouse prepares：Greenhouse is cultivated, light transmittance should be with
Shade with the enhancing of sunlight, keep shade luminous intensity, sets culture pond in greenhouse, culture base-material depth 0.5~0.6 in pond
Rice；Four, culture medium goes out miscellaneous bacteria：Culture base-material was heated to 70 DEG C~80 DEG C after one minute reduces temperature to 20 DEG C~30 naturally
℃；Five, culture base-material fills out pond and plantation：Culture base-material after high-temperature sterilization is filled into culture pond, loading depth 20cm~
Black pine conidia powder is sprinkled into when 30cm, surface sprinkles Lasiosphaera fenzlii strain conidia powder, spreading density：1g~1.5g/m2, it is further continued for loading
Culture base-material 30cm~20cm is slightly flattened with tablet, and clear water is sprayed after top dibbling cucumber seeds to humidity；Six, strain is given birth to
Long-standing management：The mainly growth of management culture medium top cucumber, builds cucumber holder, keeps the darkness tide of culture base-material
It is wet, it keeps soil moisture content to be not less than 16%, suitably sprays water when dry；25 DEG C~30 DEG C conducts of canopy temperature are kept to urge the mushroom phase
Temperature；Seven, fruiting time：Black pine growth time was best with 35~40 days, and cucumber also just ties okra fruit at this time, picks cucumber
The black pine thalline of underground is dug out afterwards.
Claims (1)
1. a kind of artificial culture method of black pine strain, it is characterized in that being carried out by following step：One, strain prepares：It will be at
Ripe black pine thalline peeling, extraction thalline entity grind the conidia powder that powdering is sporinite or sporinite aggregate；Two, it trains
It is standby to support benchmark：40~50 parts of dry cornstalk slag, 20~30 parts of wood dust, grass cuttings are 30~40 parts broken, 20~30 parts of dry clay, thin
15~20 parts of sand, 8~10 parts of cow dung, and be uniformly mixed as culture base-material；Three, culture greenhouse prepares：Cultivate greenhouse, light transmittance
It should be shaded with the enhancing with sunlight, keep shade luminous intensity, set culture pond in greenhouse, culture base-material depth 0.5 in pond
~0.6 meter；Four, culture medium goes out miscellaneous bacteria：Culture base-material was heated to 70 DEG C~80 DEG C after one minute reduces temperature to 20 DEG C naturally
~30 DEG C；Five, culture base-material fills out pond and plantation：Culture base-material after high-temperature sterilization is filled into culture pond, loading depth 20cm
Black pine conidia powder is sprinkled into when~30cm, surface sprinkles Lasiosphaera fenzlii strain conidia powder, spreading density：1g~1.5g/m2, it is further continued for filling out
Culture base-material 30cm~20cm is filled, is slightly flattened with tablet, clear water is sprayed after top dibbling cucumber seeds to humidity；Six, strain
The management of growth period：The mainly growth of management culture medium top cucumber, builds cucumber holder, keeps the darkness of culture base-material
Humidity keeps soil moisture content to be not less than 16%, suitably sprays water when dry；25 DEG C~30 DEG C conducts of canopy temperature are kept to urge mushroom
The temperature of phase；Seven, fruiting time：Black pine growth time was best with 35~40 days, and cucumber also just ties okra fruit at this time, and picking is yellow
The black pine thalline of underground is dug out after melon.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611068010.4A CN108370830A (en) | 2016-11-28 | 2016-11-28 | A kind of artificial culture method of black pine strain |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611068010.4A CN108370830A (en) | 2016-11-28 | 2016-11-28 | A kind of artificial culture method of black pine strain |
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| Publication Number | Publication Date |
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| CN108370830A true CN108370830A (en) | 2018-08-07 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201611068010.4A Withdrawn CN108370830A (en) | 2016-11-28 | 2016-11-28 | A kind of artificial culture method of black pine strain |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110583359A (en) * | 2019-10-09 | 2019-12-20 | 四川省农业科学院土壤肥料研究所 | Truffle in-situ tending microbial inoculum and preparation and use method thereof |
| TWI714499B (en) * | 2020-04-21 | 2020-12-21 | 耕詮股份有限公司 | Method for inoculating economically valuable truffles to Castanea plants to form mycorrhizal seedlings |
-
2016
- 2016-11-28 CN CN201611068010.4A patent/CN108370830A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110583359A (en) * | 2019-10-09 | 2019-12-20 | 四川省农业科学院土壤肥料研究所 | Truffle in-situ tending microbial inoculum and preparation and use method thereof |
| TWI714499B (en) * | 2020-04-21 | 2020-12-21 | 耕詮股份有限公司 | Method for inoculating economically valuable truffles to Castanea plants to form mycorrhizal seedlings |
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| Date | Code | Title | Description |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20180807 |
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| WW01 | Invention patent application withdrawn after publication |