CN108362514A - A kind of elegant jessamine root nodule quick sampling method - Google Patents
A kind of elegant jessamine root nodule quick sampling method Download PDFInfo
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- CN108362514A CN108362514A CN201810110800.7A CN201810110800A CN108362514A CN 108362514 A CN108362514 A CN 108362514A CN 201810110800 A CN201810110800 A CN 201810110800A CN 108362514 A CN108362514 A CN 108362514A
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- root
- elegant jessamine
- root nodule
- nodule
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
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Abstract
The present invention provides a kind of elegant jessamine root nodule quick sampling method, includes the following steps：Step S1：Choose elegant jessamine to be sampled；Step S2：Tendril along the elegant jessamine finds adventitious root, and root nodule is acquired in the system of fibrous root of the adventitious root.A kind of elegant jessamine root nodule quick sampling method provided by the invention, it is to find root nodule in the system of fibrous root of adventitious root, root nodule can be found in 30min, compared to the method for traditional acquisition root nodule, the time for finding root nodule can be greatly reduced, it is time saving and energy saving, the efficiency excavated and find elegant jessamine root nodule is improved, and root nodule sample high-quality, that activity is high can be obtained.
Description
Technical field
The present invention relates to elegant jessamine root nodule acquisition technique field more particularly to a kind of elegant jessamine root nodule quick sampling methods.
Background technology
Elegant jessamine is pulse family Pueraria lobota category perennial plant, and to wind liana, stem herbaceous stem or base portion are wooden, are often distributed in grass in flakes
Slope shrubbery, opening and border etc..Elegant jessamine can establish symbiosis with rhizobium, improve soil by biological nitrogen fixation
Structure is increase soil fertility, and is the ideal species for carrying out restoration of the ecosystem.Efficient Quick Acquisition elegant jessamine root nodule is that research and elegant jessamine are total
The important prerequisite of raw rhizobium Species structure and nitrogen-fixing efficiency, for give full play to its advantageous fixed nitrogen ecological effect and
The soil fertility promotion of degraded ecosystem has great importance.
The common method of existing acquisition root nodule is first to remove withering and falling for upper soll layer with small scoop after searching out target plant
Object etc., then gently excavated within the scope of 15-20cm around the main root, find root nodule.Since elegant jessamine is perennial plant, tendril landing
Many adventitious roots can be formed, root system is quite flourishing, and this method Radix Puerariae tumor in the case where acquiring natural ecosystems environment generally requires
It takes a substantial amount of time and manpower.Currently, there is not yet the standardization method of the efficient Quick Acquisition elegant jessamine root nodule of document report.
Therefore, for the defect of the existing method of sampling, a kind of method rapidly and efficiently acquiring elegant jessamine root nodule is established, for elegant jessamine rhizobium
Research it is most important.
Invention content
It is an object of the invention in order to solveThe existing elegant jessamine root nodule method of sampling is time-consuming and laborious, it is long to expend the timeAsk
Topic, provides a kind of elegant jessamine root nodule quick sampling method.
A kind of elegant jessamine root nodule quick sampling method provided by the invention, includes the following steps：
Step S1：Choose elegant jessamine to be sampled；
Step S2：Tendril along the elegant jessamine finds adventitious root, and root nodule is acquired in the system of fibrous root of the adventitious root.
The present invention finds root nodule in the system of fibrous root of adventitious root, and root nodule can be found in 30min, and tradition acquires
The method of root nodule is the searching root nodule at main root, and root nodule can just be found by taking 1~2h, therefore the present invention is compared to traditional acquisition
The time for finding root nodule can be greatly reduced in the method for root nodule, time saving and energy saving, improve the efficiency excavated and find elegant jessamine root nodule.
Preferably, in the step S1, the elegant jessamine being distributed in flakes is chosen as elegant jessamine to be sampled；Or choose be grown in it is thin
Elegant jessamine in pine, sand environment is as elegant jessamine to be sampled.
Elegant jessamine likes to be born in the rainy place on the sunny side of warm moist, is often distributed in barren hill or bushes, the shape of the blade of elegant jessamine
It is larger that looks feature is mainly rendered as blade, and terminal leaflet is in water chestnut shape width egg shape, and apex is tapering, basal circular, and the leaflet of both sides is in
Width egg shape, base portion is oblique, and stipule is in peltate, and stipel is in needle-shaped, since the blade of elegant jessamine is larger and the shape characteristic of its blade
More apparent, sheet of elegant jessamine is easier to be found, and the elegant jessamine being distributed in flakes, and due to its well developed root system, soil is more loose, just
In sampling, whether sheet of plant has elegant jessamine on the barren hill that can face south when sampling along road driving searching both sides of the road, can
To greatly shorten searching and acquire the time of elegant jessamine root nodule.When elegant jessamine distribution is scattered not in flakes, then can select to choose it is loose,
Elegant jessamine in sand, root nodule generally grow relatively abundanter, the nodule number in hardened soil in loose, sand
Less and it is small, acquisition root nodule is difficult, therefore chooses loose, sand environment, on the one hand can save the time that searching acquires root nodule,
On the other hand high-quality root nodule can be obtained.
Preferably, in the step S2, diameter d >=3mm is acquired in the system of fibrous root of the adventitious root, cross section is pink
The root nodule of color.
The root nodule diameter of elegant jessamine is often between 1mm~5mm, it is considered that more than 3mm is a big root nodule, pink
Refer to the crosscutting face color of root nodule, a big, section is that the root nodules nitrogenase activity of pink is higher, it is easier to isolate rhizobium.
Preferably, further include step S3：The root nodule is placed in the sealing container equipped with discoloration silica gel at 4~6 DEG C
It preserves.
Rhizobium can survive 10~15 days under air drying state, and it is to ensure that root nodule, which is stored in discoloration silica gel,
The drying of root nodule can replace discoloration silica gel immediately when the silica gel that changes colour becomes pink completely by blue, to ensure that root nodule begins
Eventually under dry environment.It is placed in the activity for being preserved under the conditions of 4~6 DEG C and can preferably ensureing rhizobium, extends rhizobium
Time-to-live.
The beneficial effects of the present invention are：A kind of elegant jessamine root nodule quick sampling method provided by the invention, is in adventitious root
The system of fibrous root on find root nodule, root nodule can be found in 30min, can be with compared to the method for traditional acquisition root nodule
Be greatly reduced find root nodule time, it is time saving and energy saving, improve excavate find elegant jessamine root nodule efficiency, and can obtain it is high-quality,
The high root nodule sample of activity.
Description of the drawings
Fig. 1 is the elegant jessamine image that the present invention samples；
Fig. 2 is the adventitious root system of fibrous root image for the elegant jessamine that the present invention samples；
Fig. 3 is root nodule morphological image in the adventitious root system of fibrous root for the elegant jessamine that the present invention samples；
Fig. 4 is the root nodule image of the pink of acquisition；
Fig. 5 is the preservation image of the root nodule of acquisition.
Specific implementation mode
It in order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below will be in the embodiment of the present invention
Technical solution be explicitly described, it is clear that described embodiments are some of the embodiments of the present invention, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are obtained without making creative work
The every other embodiment obtained, shall fall within the protection scope of the present invention.Unless otherwise specified, technological means used is in embodiment
Conventional means well-known to those skilled in the art.
Embodiment 1
1, sample devices
The small scoop of stainless steel, scissors, tweezers, GPS positioning instrument, digital camera, seed packet (or kraft envelope), mark
Pen, discoloration silica gel centrifuge tube.
2, the method for sampling
1) in sampling area range (Guangxi Huan Jiangxian Karst stony mountain areas), both sides of the road is searched along road and are faced south on barren hill
Plant, according to elegant jessamine section belong to feature find, identification elegant jessamine, preferentially choose the elegant jessamine that is distributed in flakes as acquiring root nodule and wait adopting
The elegant jessamine (as shown in Figure 1) of sample, record in detail collecting location, the ecological environment around collection point, plant growth period and
Rough soil types etc..
2) adventitious root is found along the tendril of elegant jessamine, the Litter-fall impurity on adventitious root surrounding soil surface layer is removed with small scoop
Deng, then gently stir with small wooden soil and become loose to extract the complete adventitious root system of fibrous root (the adventitious root system of fibrous root by it
As shown in Figure 2), acquisition root nodule is found in the system of fibrous root, and surrounding's ecological environment of the plant, plant growth state are shot with camera
(florescence or fruiting period etc.) and root nodule form (as shown in Figure 3).It locks after elegant jessamine to be sampled to root nodule is collected, takes
25min。
3) after finding root nodule, gently shake off the soil on root system, cut diameter d >=3mm with scissors, cross section is pink
The root nodule (retaining the root of 2~3cm long, to ensure the complete of root nodule, as shown in Figure 4) of color.
4) root nodule is put into the 15mL centrifuge tubes for being pre-loaded with semicanal discoloration silica gel, posts sample label, takes back experiment
Room carries out pretreatment and is placed on preservation (as shown in Figure 5) in 4~6 DEG C of refrigerator.
5) often centrifuge tube is checked, it is found that when silica gel changes colour completely, root nodule should be transferred to new be equipped in time
In the 15mL centrifuge tubes of semicanal discoloration silica gel.
Finally it should be noted that：The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although
Present invention has been described in detail with reference to the aforementioned embodiments, it will be understood by those of ordinary skill in the art that：It still may be used
With technical scheme described in the above embodiments is modified or equivalent replacement of some of the technical features；
And these modifications or replacements, various embodiments of the present invention technical solution that it does not separate the essence of the corresponding technical solution spirit and
Range.
Claims (4)
1. a kind of elegant jessamine root nodule quick sampling method, which is characterized in that include the following steps：
Step S1：Choose elegant jessamine to be sampled；
Step S2：Tendril along the elegant jessamine finds adventitious root, and root nodule is acquired in the system of fibrous root of the adventitious root.
2. elegant jessamine root nodule quick sampling method as described in claim 1, which is characterized in that in the step S1, choose in blocks
The elegant jessamine of distribution is as elegant jessamine to be sampled；Or it chooses and is grown in elegant jessamine loose, in sand environment as to be sampled
Elegant jessamine.
3. elegant jessamine root nodule quick sampling method as described in claim 1, which is characterized in that in the step S2, it is described not
Determine the root nodule for diameter d >=3mm being acquired in the system of fibrous root of root, cross section is pink.
4. elegant jessamine root nodule quick sampling method as described in claim 1, which is characterized in that further include step S3：By described
Tumor is placed in the sealing container equipped with discoloration silica gel and is preserved at 4~6 DEG C.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810110800.7A CN108362514A (en) | 2018-02-05 | 2018-02-05 | A kind of elegant jessamine root nodule quick sampling method |
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| CN201810110800.7A CN108362514A (en) | 2018-02-05 | 2018-02-05 | A kind of elegant jessamine root nodule quick sampling method |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1936551A (en) * | 2006-01-13 | 2007-03-28 | 中国科学院东北地理与农业生态研究所 | Method for detecting root nodule anlage of bean family crops |
| CN103069947A (en) * | 2013-02-27 | 2013-05-01 | 中国热带农业科学院橡胶研究所 | Acidified soil improvement method for rubber plantation |
| CN105453923A (en) * | 2015-12-08 | 2016-04-06 | 甘肃农业大学 | Method for promoting target rhizobium to migrate and colonize in alfalfa plant |
-
2018
- 2018-02-05 CN CN201810110800.7A patent/CN108362514A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1936551A (en) * | 2006-01-13 | 2007-03-28 | 中国科学院东北地理与农业生态研究所 | Method for detecting root nodule anlage of bean family crops |
| CN103069947A (en) * | 2013-02-27 | 2013-05-01 | 中国热带农业科学院橡胶研究所 | Acidified soil improvement method for rubber plantation |
| CN105453923A (en) * | 2015-12-08 | 2016-04-06 | 甘肃农业大学 | Method for promoting target rhizobium to migrate and colonize in alfalfa plant |
Non-Patent Citations (4)
| Title |
|---|
| J.M.芬森特: "《根瘤菌实用研究手册》", 31 January 1974 * |
| 姚崇舜: "《葛根的药理作用与临床应用》", 30 April 2014, 人民军医出版社 * |
| 熊力夫 等: "野葛植物学特征与生物学特征性观察", 《湖南农业科学》 * |
| 黄宝灵 等: "几种罗汉松属植物根瘤的形态与结构", 《广西植物》 * |
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