CN108359598A - A kind of sleep disturbance gene tester and its application - Google Patents
A kind of sleep disturbance gene tester and its application Download PDFInfo
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- CN108359598A CN108359598A CN201810038920.0A CN201810038920A CN108359598A CN 108359598 A CN108359598 A CN 108359598A CN 201810038920 A CN201810038920 A CN 201810038920A CN 108359598 A CN108359598 A CN 108359598A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
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Abstract
The present invention relates to a kind of sleep disturbance gene tester and its applications, include the following steps:Blood of human body to be tested is extracted, and gene extracting solution and protease is added, obtains preliminary mixed liquor;Preliminary mixing liquid is fitted into extraction cup;It is vibrated using vibrating disk to obtaining preliminary mixed liquor in extraction cup, obtains later stage mixed liquor;The later stage mixed liquor obtained after vibration is put into separator and is separated by electrophoresis;The liquid containing sleep disturbance gene after electrophoretic separation is respectively charged into each test tube, and is inserted into the slot of detection case respectively in sequence;Open the start button of gene assaying device;It selects the liquid in corresponding test tube to be detected by select button, opens the Controlled valve in corresponding test tube, extracted by suction pump in the liquid to detection module in corresponding test tube, carry out genetic test;The result detected is included in display module by genetic test module.
Description
Technical field
The invention belongs to genetic test fields, and in particular to a kind of sleep disturbance gene tester and its application.
Background technology
Current technique of gene detection is all to be detected by gene detecting chip or genetic test test paper, still
This detection is needed through human assistance, and detection speed is slow, efficiency is low, can not detect most genes simultaneously, and testing result
Accuracy it is also less high.
In consideration of it, proposing a kind of sleep disturbance gene tester and its project to be studied of invention that bases on practicality.
Invention content
In view of the above-mentioned problems, the purpose of the present invention is to provide a kind of sleep disturbance gene tester and its application, purport
Solving the problems, such as that prior art detection speed is slow, efficiency is low, can not detect most genes and testing result accuracy is low.
To achieve the goals above, the present invention uses following technical scheme:A kind of sleep disturbance gene tester, in advance
Prepare following gene assaying device, the gene assaying device include a workbench, a vibrating disk, an extraction cup, a detection case,
Several test tubes, a suction pump and a genetic test mechanism;
The detection case and extraction cup are respectively provided on the table, and the vibrating disk is located at extraction cup and obtains bottom to extraction cup progress
Vibration, the detection case is interior to be equipped with several slots, and a test tube, each test tube and genetic test mechanism are inserted in each slot
Between be equipped with a connection channel, a Controlled valve is equipped in each interface channel;
The outside of the genetic test mechanism setting detection case, and include a detection module, a suction pump, a control module, one
Network server, a network module and a display module, the detection module, suction pump, Controlled valve and display module are equal
It is connected to control module;The control module has the first control terminal and several second control terminals, and the of the control module
One control terminal is connected to suction pump, several second control terminals of the control module are connected to correspondingly in several test tubes
Controlled valve;Several select buttons are equipped in the control module;
Follow the steps below operation:
The first step extracts blood of human body to be tested, and gene extracting solution and protease is added, and obtains preliminary mixed liquor;
Preliminary mixing liquid is fitted into extraction cup by second step;
Third walks, and is vibrated using vibrating disk to obtaining preliminary mixed liquor in extraction cup, obtains later stage mixed liquor;
The later stage mixed liquor obtained after vibration is put into separator and is separated by electrophoresis by the 4th step;
The liquid containing sleep disturbance gene after electrophoretic separation is respectively charged into each test tube by the 5th step, and in sequence
It is inserted into the slot of detection case respectively
6th step opens the start button of gene assaying device;
7th step selects the liquid in corresponding test tube to be detected, opens the Controlled valve in corresponding test tube, lead to by select button
It crosses in the liquid to detection module that suction pump is extracted in corresponding test tube, carries out genetic test;
The result detected is included in the display module by the 8th step, the genetic test module.
As a further improvement on the present invention, further include the 9th step, the genetic test module leads to the result detected
It crosses network module and sends network server to, personal investigation checks for coherent detection.
As a further improvement on the present invention, the control module includes a central processing element.
As a further improvement on the present invention, the central processing element uses TMS320DM642 chips.
As a further improvement on the present invention, the display module uses LCD liquid crystal display screen.
To achieve the goals above, the present invention uses following another technical solution:A kind of sleep disturbance genetic test side
The application of method carries out genetic test using above-described detection method to sleep disturbance gene to be detected.
The present invention relates to a kind of sleep disturbance gene tester and its application, detection speed is fast, efficient while can be with
The most genes of detection, and also ensure that the result of detection is accurate.
Description of the drawings
Attached drawing described here is only used for task of explanation, and is not intended to limit model disclosed in the present application in any way
It encloses.In addition, the shape and proportional sizes etc. of each component in figure are only schematical, it is used to help the understanding to the application, and
It is not the specific shape and proportional sizes for limiting each component of the application.Those skilled in the art, can under teachings of the present application
Implement the application to select various possible shapes and proportional sizes as the case may be.In the accompanying drawings:
Attached drawing 1 is the method flow schematic diagram of the present invention
Specific implementation mode
The present invention will be further illustrated in following example.These embodiments are merely to illustrate the present invention, but not to appoint
Where formula limitation is of the invention.
Embodiment:A kind of sleep disturbance gene tester and its application
Referring to attached drawing 1, prepare following gene assaying device in advance, the gene assaying device include a workbench, a vibrating disk,
One extraction cup, a detection case, several test tubes, a suction pump and a genetic test mechanism.
The detection case and extraction cup are respectively provided on the table, and the vibrating disk is located at extraction cup and obtains bottom to extraction cup
It is vibrated, several slots is equipped in the detection case, a test tube, each test tube and genetic test are inserted in each slot
It is equipped with a connection channel between mechanism, a Controlled valve is equipped in each interface channel.
The outside of genetic test mechanism setting detection case, including a detection module, a suction pump, a control module,
One network server, a network module and a display module, the detection module, suction pump, Controlled valve and display module
It is connected to control module;The control module has the first control terminal and several second control terminals, the control module
First control terminal is connected to suction pump, several second control terminals of the control module are connected to several test tubes correspondingly
In Controlled valve;Several select buttons are equipped in the control module.
Follow the steps below operation:
The first step extracts blood of human body to be tested, and gene extracting solution and protease is added, and obtains preliminary mixed liquor;
Preliminary mixing liquid is fitted into extraction cup by second step;
Third walks, and is vibrated using vibrating disk to obtaining preliminary mixed liquor in extraction cup, obtains later stage mixed liquor;
The later stage mixed liquor obtained after vibration is put into separator and is separated by electrophoresis by the 4th step;
The liquid containing sleep disturbance gene after electrophoretic separation is respectively charged into each test tube by the 5th step, and in sequence
It is inserted into the slot of detection case respectively
6th step opens the start button of gene assaying device;
7th step selects the liquid in corresponding test tube to be detected, opens the Controlled valve in corresponding test tube, lead to by select button
It crosses in the liquid to detection module that suction pump is extracted in corresponding test tube, carries out genetic test;
8th step, the genetic test module by the result detected include in the display module, and count in statistical form
In;
9th step, the genetic test module sends the result detected to network server by network module, for phase
Testing staff is closed to check.
In the present embodiment, the control module includes a central processing element, and the central processing element uses
TMS320DM642 chips.
In addition, the genetic test mechanism further includes network server and network module;The genetic test result can be with
Network server is sent to by network module.
The present invention relates to a kind of sleep disturbance gene tester and its application, detection speed is fast, efficient while can be with
The most genes of detection, and also ensure that the result of detection is accurate.
It should be noted that in the description of the present application, unless otherwise indicated, the meaning of " plurality " is two or two with
On.
Using term "comprising" or " comprising " come describe element here, ingredient, component or step combination it is also contemplated that
Substantially the embodiment being made of these elements, ingredient, component or step.Here by using term " can with ", it is intended to illustrate
Described any attribute that " can with " includes all is optional.
Multiple element, ingredient, component or step can be provided by single integrated component, ingredient, component or step.Optionally
Ground, single integrated component, ingredient, component or step can be divided into multiple element, ingredient, component or the step of separation.It is used for
The open "a" or "an" for describing element, ingredient, component or step is not said to exclude other elements, ingredient, component
Or step.
It should be understood that above description is to illustrate rather than to be limited.By reading above-mentioned retouch
It states, many embodiments and many applications except the example provided all will be aobvious and easy for a person skilled in the art
See.Therefore, the range of this introduction should not be determined with reference to foregoing description, but should with reference to preceding claims and this
The full scope of the equivalent that a little claims are possessed determines.For comprehensive purpose, all articles and with reference to including special
The disclosure of profit application and bulletin is all by reference to being incorporated herein.Theme disclosed herein is omitted in preceding claims
Any aspect is not intended to abandon the body matter, also should not be considered as applicant the theme is not thought of as it is disclosed
Apply for a part for theme.
The series of detailed descriptions listed above only for the application feasible embodiment specifically
Bright, they are all without departing from equivalent implementations made by the application skill spirit not to limit the protection domain of the application
Or change should be included within the protection domain of the application.
Claims (6)
1. a kind of sleep disturbance gene tester, it is characterised in that:Prepare following gene assaying device, the gene inspection in advance
It includes a workbench, a vibrating disk, an extraction cup, a detection case, several test tubes, a suction pump and a genetic test to survey device
Mechanism;
The detection case and extraction cup are respectively provided on the table, and the vibrating disk is located at extraction cup and obtains bottom to extraction cup progress
Vibration, the detection case is interior to be equipped with several slots, and a test tube, each test tube and genetic test mechanism are inserted in each slot
Between be equipped with a connection channel, a Controlled valve is equipped in each interface channel;
The outside of the genetic test mechanism setting detection case, and include a detection module, a suction pump, a control module, one
Network server, a network module and a display module, the detection module, suction pump, Controlled valve and display module are equal
It is connected to control module;The control module has the first control terminal and several second control terminals, and the of the control module
One control terminal is connected to suction pump, several second control terminals of the control module are connected to correspondingly in several test tubes
Controlled valve;Several select buttons are equipped in the control module;
Follow the steps below operation:
The first step extracts blood of human body to be tested, and gene extracting solution and protease is added, and obtains preliminary mixed liquor;
Preliminary mixing liquid is fitted into extraction cup by second step;
Third walks, and is vibrated using vibrating disk to obtaining preliminary mixed liquor in extraction cup, obtains later stage mixed liquor;
The later stage mixed liquor obtained after vibration is put into separator and is separated by electrophoresis by the 4th step;
The liquid containing sleep disturbance gene after electrophoretic separation is respectively charged into each test tube by the 5th step, and in sequence
It is inserted into the slot of detection case respectively;
6th step opens the start button of gene assaying device;
7th step selects the liquid in corresponding test tube to be detected, opens the Controlled valve in corresponding test tube, lead to by select button
It crosses in the liquid to detection module that suction pump is extracted in corresponding test tube, carries out genetic test;
The result detected is included in the display module by the 8th step, the genetic test module.
2. sleep disturbance gene tester according to claim 1, it is characterised in that:Further include the 9th step, the base
Because detection module sends the result detected to network server by network module, for coherent detection, personal investigation looks into
It sees.
3. sleep disturbance gene tester according to claim 1 or 2 is used, it is characterised in that:The control module packet
Include a central processing element.
4. sleep disturbance gene tester according to claim 3 is used, it is characterised in that:The central processing element is adopted
With TMS320DM642 chips.
5. sleep disturbance gene tester according to claim 1, it is characterised in that:The display module uses LCD
Liquid crystal display.
6. a kind of application of sleep disturbance gene tester, it is characterised in that:Using detection side as described in claim 1
Method carries out genetic test to sleep disturbance gene to be detected.
Priority Applications (1)
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CN201810038920.0A CN108359598A (en) | 2018-01-19 | 2018-01-19 | A kind of sleep disturbance gene tester and its application |
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CN201810038920.0A CN108359598A (en) | 2018-01-19 | 2018-01-19 | A kind of sleep disturbance gene tester and its application |
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Publication Number | Publication Date |
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CN108359598A true CN108359598A (en) | 2018-08-03 |
Family
ID=63006184
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CN201810038920.0A Withdrawn CN108359598A (en) | 2018-01-19 | 2018-01-19 | A kind of sleep disturbance gene tester and its application |
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Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103282782A (en) * | 2010-10-27 | 2013-09-04 | 株式会社百奥尼 | Automatic real-ime pcr system for the various analysis of biological smaple |
CN105709299A (en) * | 2016-04-06 | 2016-06-29 | 广西水利电力职业技术学院 | Medical transfusion liquid level real-time monitoring system and method based on wireless transmission |
CN106434318A (en) * | 2016-06-18 | 2017-02-22 | 泉州市知产茂业工业设计有限公司 | Gene detection device adopting biotechnology |
-
2018
- 2018-01-19 CN CN201810038920.0A patent/CN108359598A/en not_active Withdrawn
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103282782A (en) * | 2010-10-27 | 2013-09-04 | 株式会社百奥尼 | Automatic real-ime pcr system for the various analysis of biological smaple |
CN105709299A (en) * | 2016-04-06 | 2016-06-29 | 广西水利电力职业技术学院 | Medical transfusion liquid level real-time monitoring system and method based on wireless transmission |
CN106434318A (en) * | 2016-06-18 | 2017-02-22 | 泉州市知产茂业工业设计有限公司 | Gene detection device adopting biotechnology |
Non-Patent Citations (1)
Title |
---|
王华民等主编: "《临床免疫学》", 31 December 2010 * |
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Application publication date: 20180803 |