CN108353889B - Traditional Chinese medicine type nano titanium dioxide specimen preserving fluid - Google Patents

Traditional Chinese medicine type nano titanium dioxide specimen preserving fluid Download PDF

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CN108353889B
CN108353889B CN201810272115.4A CN201810272115A CN108353889B CN 108353889 B CN108353889 B CN 108353889B CN 201810272115 A CN201810272115 A CN 201810272115A CN 108353889 B CN108353889 B CN 108353889B
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titanium dioxide
specimen
nano titanium
extract
chinese medicine
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CN108353889A (en
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祖文轩
刘山
赵晨
王玉华
郭梁影
杨俊松
蒋邦红
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BENGBU MEDICAL COLLEGE
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/02—Acyclic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/16—Heavy metals; Compounds thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint

Abstract

The invention discloses a traditional Chinese medicine type nano titanium dioxide specimen preserving fluid which comprises the following raw materials: honeysuckle extract, salvia extract, nano titanium dioxide, glacial acetic acid, auxiliary components and water. The invention takes glacial acetic acid, honeysuckle extract, salvia extract and nano titanium dioxide as the main components of the specimen liquid, so that tissues rich in fiber in the specimen swell, and simultaneously fix protein and fat and eliminate bacteria; ethanol and glycerol are used as auxiliary components, so that the fixation is accelerated, and the organic solvent in the solution is adsorbed at the same time. The preservation solution can ensure that the specimen has no putrefaction phenomenon within one year, the skin mucosa and the like of the specimen have no obvious change, no mould is generated in the preservation solution, the specimen is well fixed, and the specimen preservation solution has no pungent smell.

Description

Traditional Chinese medicine type nano titanium dioxide specimen preserving fluid
Technical Field
The invention relates to the technical field of biological dissection, in particular to a traditional Chinese medicine type nano titanium dioxide specimen preserving fluid.
Background
In clinical anatomical experiments, the specimen is usually required to be soaked in a preservation solution so as to achieve the purposes of preserving the experimental specimen for a long time, fixing protein and fat and the like. Since 1886 formaldehyde was found to be an effective preservative, formaldehyde has been widely used as a specimen preservation solution fixative for preserving tissues, organs and whole remains. The traditional specimen preservation solution taking formaldehyde as the antiseptic fixing agent has the advantages of low price, strong sterilization capability, thorough antiseptic effect, capability of inhibiting the growth of mold and yeast, good fixing and hardening effects on tissues, capability of removing amine odor generated in remains in the decay process, simple and convenient operation method and low consumption, but formaldehyde is volatile and has high toxicity and great stimulation to respiratory mucosa and skin of experimenters; the long-term contact can also cause the damage of multiple systems and multiple organs, and even has certain carcinogenicity; and the color, texture and tissue elasticity of the specimen are kept relatively poor, and along with the gradual development of the technical fields of medicine, science and technology and environmental protection, the traditional specimen preserving fluid and the specimen preserved by the traditional preserving fluid can not meet the requirements of people in aspects of scientific research, teaching and the like gradually. How to prepare the improved preservation solution which has the characteristics of antisepsis and antibiosis of the traditional preservation solution, has small irritation to the skin mucosa of a human body and keeps the color, texture and tissue elasticity of a specimen for a long time is a technical problem which needs to be solved urgently at present, and has very important significance for the research of clinical anatomy.
Disclosure of Invention
Based on the technical problems in the background art, the invention provides the traditional Chinese medicine type nano titanium dioxide specimen preservation solution which has good antiseptic and antibacterial effects, can keep the color, texture and tissue elasticity of the specimen for a long time, and has small irritation to the skin mucosa of a human body.
The invention provides a traditional Chinese medicine type nano titanium dioxide specimen preserving fluid which comprises the following raw materials: honeysuckle extract, salvia extract, nano titanium dioxide, glacial acetic acid, auxiliary components and water.
The honeysuckle extract has the efficacies of clearing away heat and toxic material, clearing and activating the channels and collaterals, regulating immunity, broad-spectrum antibiosis, antivirus and the like, and is called as 'traditional Chinese medicine antibiotic'. The honeysuckle is rich in organic acids, volatile oil, flavonoids and other components, the organic acids are considered as main active substances for antibiosis and antisepsis, and comprise chlorogenic acid, isochlorogenic acid, caffeic acid, luteolin glycoside and the like, and the honeysuckle has strong inhibition effect on pathogenic bacteria such as typhoid bacillus, escherichia coli, proteus, pseudomonas aeruginosa, staphylococcus, dysentery bacillus, streptococcus, diplococcus pneumoniae, meningococcus and the like.
The Saviae Miltiorrhizae radix extract has effects of scavenging oxygen free radicals, inhibiting lipid peroxidation, enhancing immunity, resisting liver fibrosis, resisting tumor, and resisting aging. The salvia miltiorrhiza is rich in tanshinone IIA, salvianolic acid B, cryptotanshinone, dihydrotanshinone and other components, wherein the cryptotanshinone and the dihydrotanshinone have excellent antibacterial effect and have obvious antibacterial and bactericidal effects on escherichia coli, staphylococcus aureus, modified bacillus and streptococcus.
Preferably, the raw materials comprise the following components in percentage by weight: 5-15% of honeysuckle extract, 5-15% of salvia miltiorrhiza extract, 3-8% of nano titanium dioxide, 3-6% of glacial acetic acid, 16-28% of auxiliary components and the balance of water.
Preferably, the raw materials comprise the following components in percentage by weight: 8-12% of honeysuckle extract, 8-12% of salvia miltiorrhiza extract, 4-6% of nano titanium dioxide, 4-5% of glacial acetic acid, 19-24% of auxiliary components and the balance of water.
Preferably, the honeysuckle extract and the salvia miltiorrhiza extract are prepared by performing rotary alcohol extraction on honeysuckle and salvia miltiorrhiza.
Preferably, the auxiliary components comprise the following components in parts by weight: 15-25 parts of alcohol and 1-3 parts of glycerol.
Preferably, the alcohol in the auxiliary component is 3-8% ethanol water solution by mass fraction.
Has the advantages that: the specimen using the specimen preservation solution of the invention can be free from putrefaction phenomenon within one year, the skin mucosa and the like of the specimen have no obvious change, no mould is generated in the preservation solution, the specimen is well fixed, and the specimen preservation solution has no pungent smell.
The invention provides a traditional Chinese medicine type nano titanium dioxide specimen preservation solution, which has the advantages of higher oxidation activity, stronger stability, no toxicity to human bodies, capability of completely killing microorganisms contacted with the nano titanium dioxide in a short time under the irradiation of light rays, high sterilization efficiency, durability and no secondary pollution, and the traditional Chinese medicine honeysuckle flower extract and the salvia miltiorrhiza extract have excellent anti-inflammatory, antibacterial and antioxidant properties, long action period and no harm to human bodies, and glacial acetic acid can promote the expansion of tissues rich in fibers and reduce the high shrinkage and hardening of ethanol on the tissues, so that the specimens are softened. Nanometer titanium dioxide, honeysuckle extract, salvia extract and glacial acetic acid are used as main components of the specimen preservation solution, so that fibrous tissues in the specimen are expanded, proteins and fat are fixed, and bacteria are eliminated; ethanol and glycerol are used as auxiliary components, the ethanol can sterilize and disinfect and accelerate curing, the ethanol is a good organic solvent, the glycerol can adsorb nano titanium dioxide, honeysuckle extract, salvia miltiorrhiza extract, glacial acetic acid and the ethanol, the antibacterial, antiseptic and mildew-proof capabilities of the sample are improved, the sample can keep lasting color, texture and tissue elasticity, and the traditional Chinese medicine type nano titanium dioxide sample preservation solution does not contain formaldehyde, is low in toxicity and has no pungent smell, so that the traditional Chinese medicine type nano titanium dioxide sample preservation solution is an ideal sample preservation solution.
Drawings
FIG. 1 is a graph showing the effect of muscle fixation observed under the mirror after HE staining of upper and lower limb muscles of rabbits preserved with the preservation solution prepared in example 5 and the preservation solution of water and formaldehyde group.
Detailed Description
Example 1
A traditional Chinese medicine type nano titanium dioxide specimen preserving fluid comprises the following raw materials: honeysuckle extract, salvia miltiorrhiza extract, nano titanium dioxide, glacial acetic acid, ethanol, glycerol and water.
Example 2
A traditional Chinese medicine type nano titanium dioxide specimen preserving fluid comprises the following raw materials in percentage by weight: 5% of honeysuckle extract, 5% of salvia miltiorrhiza extract, 3% of nano titanium dioxide, 15% of 3% ethanol water solution by mass, 1% of glycerol, 3% of glacial acetic acid and the balance of water.
Example 3
A traditional Chinese medicine type nano titanium dioxide specimen preserving fluid comprises the following raw materials in percentage by weight: 15% of honeysuckle extract, 15% of salvia miltiorrhiza extract, 8% of nano titanium dioxide, 25% of 8% ethanol aqueous solution, 3% of glycerol, 6% of glacial acetic acid and the balance of water.
Example 4
A traditional Chinese medicine type nano titanium dioxide specimen preserving fluid comprises the following raw materials in percentage by weight: 8% of honeysuckle extract, 8% of salvia miltiorrhiza extract, 4% of nano titanium dioxide, 18% of 5.6% ethanol water solution by mass, 1% of glycerol, 4% of glacial acetic acid and the balance of water.
Example 5
A traditional Chinese medicine type nano titanium dioxide specimen preserving fluid comprises the following raw materials in percentage by weight: 12% of honeysuckle extract, 12% of salvia miltiorrhiza extract, 6% of nano titanium dioxide, 22% of 4% ethanol water solution by mass, 2% of glycerol, 5% of glacial acetic acid and the balance of water.
Example 6
A traditional Chinese medicine type nano titanium dioxide specimen preserving fluid comprises the following raw materials in percentage by weight: 10% of honeysuckle extract, 10% of salvia miltiorrhiza extract, 5% of nano titanium dioxide, 20% of 7% ethanol aqueous solution by mass, 2% of glycerol, 4.5% of glacial acetic acid and the balance of water.
And (5) bacterial culture experiments. In order to observe the antiseptic and antibacterial conditions of the traditional Chinese medicine type nano titanium dioxide specimen preservation solution, the specimen preservation solution prepared in example 5, water, formaldehyde and pure water are respectively 10ml and 1ml of cooling broth to be mixed through a bacterial culture test, the mixture is cultured and recorded in a culture dish, once mould is found, the record is stopped immediately, the antiseptic condition and the antibacterial ability of the mixture are observed, and the test data are as shown in the following table 1.
TABLE 1 results of bacterial culture in preservative solution
Group/time 7 days 30 days 3 months old 6 months old 9 months old
Example 5 - - - - -
Water plus formaldehyde - - - - -
Pure water +
Note: (+ visible mold formation if no visible mold is found)
As can be seen from the above table 1, the preservative and antibacterial effects of the traditional Chinese medicine type nano titanium dioxide specimen preservative fluid are consistent with those of the preservative fluid prepared by adding formaldehyde into water, and the preservative and antibacterial fluid has good antibacterial ability.
Storage solution irritation comparative experiment. 12 rabbits with weight of about 2-3 kg are taken, healthy and disease-free and randomly divided into three groups, wherein each group comprises 3 rabbits, the preservative solution 0 and 1ml in the example 5 and the preservative solution 0 and 1ml of water and formaldehyde are respectively dripped into the conjunctival sacs of the left eye and the right eye of the rabbit, the eyelids are passively closed for 5s, the local reaction conditions of the rabbit eyes from 6 days, 24 days, 48 hours and 72 hours to seven days are recorded, the test results are shown in the following table 2, and the test standards are shown in the following table 3 and table 4.
TABLE 2 Rabbit eye preservation irritation test data
Group of Score of
Example 5 7
Water plus formaldehyde 14
Pure water 1
TABLE 3 Rabbit eye irritation test Scoring Standard
Figure BDA0001612818760000051
Figure BDA0001612818760000061
TABLE 4 Rabbit eye irritation test Scoring summary Standard
Figure BDA0001612818760000062
Figure BDA0001612818760000071
Analysis of the test data in tables 2-4 above shows that: the water-added formaldehyde preservation solution has severe stimulation to the eyes of the rabbits, while the traditional Chinese medicine type nano titanium dioxide specimen preservation solution has only mild stimulation to the eyes of the rabbits, so that the stimulation of the preservation solution is greatly relieved.
And (5) performing histological detection. The upper and lower limb muscles of the rabbits preserved with the preservation solution prepared in example 5 and the water-and-formaldehyde preservation solution were HE-stained, and the muscle fixing effect was observed under a mirror, and the test results are shown in fig. 1.
Tests show that the muscle of the upper and lower limb muscle specimen of the rabbit is ruddy in color, soft in texture, good in elasticity, intact in preservation of muscle fiber, odorless, free of pollution and no dissolution of tissues; as can be seen from the figure 1, the myofibrillar cord is intact under the observation of the microscope, the cell structure is clear, the striations exist under the high power microscope, the tissue sections of the two preservation solutions are difficult to distinguish, and the muscle fixing effect is similar.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (4)

1. A traditional Chinese medicine type nano titanium dioxide specimen preservation solution is characterized by comprising the following raw materials: honeysuckle extract, salvia extract, nano titanium dioxide, glacial acetic acid, auxiliary components and water; wherein, the raw materials comprise the following components in percentage by weight: 5-15% of honeysuckle extract, 5-15% of salvia miltiorrhiza extract, 3-8% of nano titanium dioxide, 3-6% of glacial acetic acid, 16-28% of auxiliary components and the balance of water;
wherein, the auxiliary components comprise the following components in parts by weight: 15-25 parts of alcohol and 1-3 parts of glycerol.
2. The traditional Chinese medicine type nano titanium dioxide specimen preserving fluid according to claim 1, which is characterized by comprising the following raw materials in percentage by weight: 8-12% of honeysuckle extract, 8-12% of salvia miltiorrhiza extract, 4-6% of nano titanium dioxide, 4-5% of glacial acetic acid, 19-24% of auxiliary components and the balance of water.
3. The preservation solution for traditional Chinese medicine type nanometer titanium dioxide specimens according to claim 1 or 2, characterized in that the honeysuckle extract and the salvia miltiorrhiza extract are prepared by a rotary alcohol extraction method for honeysuckle and salvia miltiorrhiza.
4. The traditional Chinese medicine type nano titanium dioxide specimen preservative fluid according to claim 1, wherein the alcohol in the auxiliary component is an ethanol aqueous solution with the mass fraction of 3-8%.
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Citations (3)

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WO1996028024A1 (en) * 1995-03-09 1996-09-19 Universidad Complutense De Madrid Compositions containing dialkyl (c1-c6)-ketone peroxide for the preservation of organic tissues, and application of said compositions to the preservation and anatomical preparation of organic tissues of animal or human origin
CN104798768A (en) * 2015-04-29 2015-07-29 合肥金域医学检验所有限公司 Preservation and treatment solution of in-vitro specimen for medical research
CN104904706A (en) * 2015-04-22 2015-09-16 青岛农业大学 Formaldehyde-free animal specimen fixed liquid and preparation method thereof

Patent Citations (3)

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Publication number Priority date Publication date Assignee Title
WO1996028024A1 (en) * 1995-03-09 1996-09-19 Universidad Complutense De Madrid Compositions containing dialkyl (c1-c6)-ketone peroxide for the preservation of organic tissues, and application of said compositions to the preservation and anatomical preparation of organic tissues of animal or human origin
CN104904706A (en) * 2015-04-22 2015-09-16 青岛农业大学 Formaldehyde-free animal specimen fixed liquid and preparation method thereof
CN104798768A (en) * 2015-04-29 2015-07-29 合肥金域医学检验所有限公司 Preservation and treatment solution of in-vitro specimen for medical research

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Title
中药抑菌及其在日化产品中的应用;张慧明等;《第九届中国化妆品学术研讨会论文集》;20120925;第352-357页 *

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