CN108342346B - Pseudomonas monteilii and application thereof - Google Patents
Pseudomonas monteilii and application thereof Download PDFInfo
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Abstract
The invention belongs to the field of environmental management, and particularly relates to pseudomonas mendocina and application thereof. The Pseudomonas monteilii is specifically Pseudomonas monteilii (Pseudomonas monteilii) LW-1 with the preservation number of CGMCC No.13748, the strain is used for treating organic sulfur-containing waste gas generated by a pesticide waste water storage pool, and the gas flow is 100-5000 m-3The residence time is 1.0-5.0min, the temperature is 35-55 ℃, and the total concentration of malathion, parathion and methyl parathion is 20-200mg/m3Or the concentration of chloral is 5-100mg/m3The degradation efficiency of malathion, parathion and methyl parathion can reach more than 90 percent, and the degradation efficiency of chloral can reach more than 75 percent.
Description
the technical field is as follows:
The invention belongs to the field of environmental management, and particularly relates to Pseudomonas monteilii (Pseudomonas monteilii) LW-1 and application thereof in purification of volatile organic sulfur/organic chlorine waste gas.
Background art:
the wastewater in the pesticide production contains a large amount of organic sulfur pesticide and organic chlorine pesticide intermediates, and has complex components, high concentration and strong toxicity. Some of the products of organic sulfur pesticides and organic chlorine pesticides or intermediates thereof such as parathion, malathion, methyl parathion, chloral, phosphorus trichloride, etc. have volatility, and most of the organic sulfur and organic chlorine pesticides have offensive odors.
Para sulfurphosphorus (C)10H14NO5PS) was a colorless oily liquid with a solubility in water of 24mg/L (25 ℃). Slightly soluble in petroleum ether and kerosene, and miscible with most organic solvents, and has garlic odor. Hydrolysis rapidly in alkaline medium; the product is stable in neutral or slightly acidic solution; is unstable to both ultraviolet light and air. It has mutagenicity to microorganism and teratogenicity to animal. Belongs to high toxicity category, and has acute toxicity, irritation and mutagenicity. Affecting the sister chromatid exchange of human lymphocytes at 200 mug/L; affecting human fibroblast in-vitro DNA synthesis at 10 μmol/L; rat lymphocyte DNA was damaged within 16 hours at 10. mu. mol/L, and IARC carcinogenicity was evaluated as grade G3; rat oral minimum toxic dose (TDLo): 360 mu g/kg (2-22 days of pregnancy/15 days of postpartum) affects the biochemistry and metabolism of the newborn mouse; subcutaneous lowest toxic dose (TDLo) in rats: 9800 mu g/kg (pregnancy 7-13 days) to cause dead fetus. Parathion can enter human body through digestive tract, respiratory tract and intact skin and mucosa. Environmental parathion can also be biologically enriched through the food chain.
Malathion (C)10H19O6PS2) It is colorless or yellowish oily liquid with garlic odor. The fuel can be burnt in case of open fire and high heat; reacting with a strong oxidant; the oxide gas of phosphorus and sulfur is generated by thermal decomposition; it is toxic to animals and irritating to eyes and skin; it is active at pH below 5.0 and susceptible to hydrolysis failure at pH above 7.0. The female rat acute oral LD50 is 1751.5mg/kg, the male rat acute oral LD50 is 1634.5mg/kg, and the rat acute percutaneous LD50 is 4000-6150 mg/kg. The toxic symptoms include headache, dizziness, nausea, weakness, hyperhidrosis, emesis, sialorrhea, blurred vision, miosis, spasm, coma, muscle fibrillation, pulmonary edema, etc. The diseases are generally quick after contacting (oral administration, inhalation, skin and mucous membrane) a large amount of organophosphorus pesticides in a short time. The incubation period of malathion intoxication is longer. Absorbed through skin, the disease occurs within 12h, and the toxic symptoms are manifested by anorexia, nausea, emesis, abdominal pain, hyperhidrosis, blurred vision, miosis, respiratory tract secretion increase, bronchospasm dyspnea, and pulmonary edema; the symptoms are manifested by fasciculation, muscle spasm and muscle paralysis; the central nervous system symptoms are headache, dizziness,Insomnia, lethargy, debilitation, dysphoria, convulsion, coma, and encephaledema. The illness is heavier when the medicine is poisoned by oral administration, and the gastrointestinal tract symptoms are more obvious. In some cases, there may be damage to the heart muscle, liver and kidney.
Methyl parathion (C)8H10NO5PS): colorless crystalline powder, brown or yellow liquid or solid as industrial product, is combustible in case of open fire and high heat, and can be decomposed quickly in alkali solution to release toxic gases such as phosphorus and sulfur oxides. The acute oral LD50 is 14-24 mg/kg for white rats, and the acute percutaneous LD50 is 300-400 mg/kg for rabbits, and belongs to high-toxicity pesticides. Methyl parathion has the effects of contact killing and stomach toxicity, can inhibit the activity of cholinesterase in the nervous system of pests to kill the pests, has a wide insecticidal spectrum, is usually processed into emulsifiable solution or powder (see pesticide formulation) for use, and is mainly used for preventing and treating various agricultural pests. Has certain mutagenicity to microorganisms, such as Salmonella typhimurium 667 μ g/dish; coli: 10 mmol/L. The gas, mist, aerosol or powder can be easily absorbed by respiratory tract, and the liquid can be absorbed by eye mucosa, skin or gastrointestinal tract. After entering into the body, the protein is rapidly distributed to various organ tissues of the whole body and is firmly combined with tissue protein.
Organic chlorine pesticides are one of the most widely used pesticides at present, and play an important role in the pesticide market. However, in the production of organochlorine pesticides and the treatment of pesticide wastewater, volatile pesticide products or intermediates volatilize from the liquid phase and escape into the air, causing air pollution.
Volatile pesticides and intermediates include: trichlorfon, dichlorvos, phosphorus trichloride, dichlobenil, dichlorfon, phorate, phoxim, biphenyl, phosphorus monofluoride, alkane and substituted alkane intermediates, aromatic intermediates and the like (organic chlorine is selected), most of the substances have pungent smell, and some substances such as diethyl phosphite and chloral belong to carcinogenic, mutagenic and teratogenic triparenes.
In the process of wastewater treatment, organic sulfur and organic chlorine with peculiar smell are dissipated into the air from water due to mechanical disturbance such as aeration, water flow pushing and the like, so that odor pollution is formed. If not properly treated, the organic chlorine dissipated into the air seriously pollutes the environment and harms the human health.
The biotechnology is one of the important methods for effectively treating the waste gas containing organic matters at present because of the advantages of low cost, simple and convenient operation, clean technology, no secondary pollution and the like. The principle of the biological treatment technology is a process for converting organic sulfur into harmless or low-harmful substances such as sulfate, carbon dioxide, water and the like by utilizing the biological oxidation of microorganisms, and the process comprises the following steps:
Malathion → carbon dioxide + sulphate + water;
Methyl parathion → p-nitrophenol + organic sulfate → sulfate + phosphate + carbon dioxide + water;
parathion → p-nitrophenol + organic sulfate → sulfate + phosphate + carbon dioxide + water.
The principle of the biological treatment technology is a process of converting organic chlorine into harmless or low-harmful substances such as carbon dioxide, hydrochloride and water by utilizing the biological oxidation of microorganisms (formula 1).
C2HCl3O+O2→CO2+H2o + HCl (formula 1)
It has been shown that natural environment facultative heterotrophic bacteria belonging to the genera Pseudomonas sp X2(201310551914.2), Sphingobacterium griseoflavus SCU-B140(201510715812.9), Acidithiobacillus ferrooxidans (Acidithiobacillus ferrooxidans), Leptospirillum ferrugineum (Leptospirillum ferrophilum) and Ferroplasia acidiphilum (201310745138.X) all have degradation effects on organic sulfur pesticides. However, Pseudomonas monteilii (Pseudomonas monteilii) has not been found to be useful for the treatment of organosulfur and organochlorine compounds, and methods for treating sulfur-and chlorine-containing contaminants using Pseudomonas monteilii.
The invention content is as follows:
in order to solve the problems of low treatment efficiency of volatile organic sulfur and organic chlorine, uneven microbial load on the surface of a mixed carrier, slow start and collapse caused by long-term use of a filler in the prior art, the invention provides Pseudomonas menengei (Pseudomonas monteilii), and the Pseudomonas menengei is applied to removal of waste gas containing organic sulfur and organic chlorine.
The Pseudomonas monteilii, specifically Pseudomonas monteilii LW-1, is a separation bacterium obtained by acclimatization and separation of pesticide wastewater containing organic sulfur such as methyl parathion, malathion, parathion and the like and organic chlorine such as phosphorus trichloride, trichloroacetaldehyde and the like. The strain is preserved in the China general microbiological culture Collection center in No. 3 and No. 10 of 2017, the preservation number is CGMCC No.13748, and the preservation address is as follows: western road No.1, north chen west road, north kyo, chaoyang, institute of microbiology, china academy of sciences, zip code 100101.
The strain is characterized in that:
Pseudomonas mendii (Pseudomonas monteilii) LW-1: the cells are rod-shaped, peritrichogenous flagellum, gram stain is negative, starch hydrolysis, VP and indole reactions are negative, and gelatin liquefaction and glucose reactions are positive. The colony is white, round, smooth in surface, wet, viscous, flat and protruding. In natural environment, the carbon source has strong competitive power and wide application range, can widely utilize carbohydrate, amino acid and organic acid as carbon sources, has wide pH application range (pH 3.0-7.0), can grow under high temperature (50 ℃ -60 ℃), has the optimal growth temperature of 30-55 ℃ and the pH of 4.0-6.5.
The invention also provides a microbial inoculum prepared from Pseudomonas monteilii LW-1, which can be used for purifying waste gas containing organic sulfur and organic chlorine and solving the pollution problem of volatile organic sulfur and organic chlorine generated in the processes of pesticide wastewater treatment and the like, and the preparation method of the microbial inoculum comprises the following steps:
(1) Preparation method of fermentation liquor
Inoculating pseudomonas menbergii LW-1 after slant culture into a liquid fermentation culture medium, and carrying out amplification culture step by step according to 10-25% of inoculum size, wherein the fermentation conditions are as follows: culturing at 35-45 deg.C with ventilation rate of 10-100L/min, dissolved oxygen of 2.0mg/L and pot pressure of 0.03-0.10Mpa for 45-55 h;
the liquid fermentation culture medium comprises: glucose 0.5g, K2HPO4 0.5g，KH2PO4 1.5g，(NH4)2SO4 0.5g，NaCl 0.5g，MgSO4 0.2g，CaCl2 0.05g，FeSO40.02g, 1000mL of water, pH 6.2-7.0, sterilization at 115 ℃ for 20min, cooling to room temperature after sterilization, and then adding 4.5mg/L of parathion and/or chloral (4.5 mg/L of parathion is added when the microbial inoculum is used for treating sulfur-containing waste gas; 4.5mg/L of chloral is added when the microbial inoculum is used for treating chlorine-containing waste gas; 4.5mg/L of parathion and 4.5mg/L of chloral are added when the microbial inoculum is used for treating sulfur-containing and chlorine-containing waste gas);
(2) Liquid microbial inoculum preparation method
Placing the fermentation liquor in a refrigerated centrifuge at 4 ℃, 2000-;
Packaging the bacterial suspension into a liquid microbial inoculum to form a pseudomonas monteilii microbial inoculum product for treating volatile organic sulfur and/or organic chlorine waste gas;
The sterile nutrient solution is as follows: glucose 0.5g, K2HPO4 0.5g，KH2PO4 1.5g，(NH4)2SO4 0.5g，NaCl 0.5g，MgCl2 0.2g，CaCl2 0.05g，FeSO40.02g, 1000mL of water, pH 6.2-7.0, sterilizing at 115 ℃ for 20 min.
The invention also provides a method for treating waste gas containing organic sulfur and/or organic chlorine by using the microbial inoculum, which comprises the following steps:
the liquid microbial inoculum is loaded on a filler and is used for treating organic sulfur and/or organic chlorine-containing waste gas generated by a pesticide waste water storage pool, and the gas flow is 100-5000m3the residence time is 1.0-5.0min, the temperature is 35-55 ℃, and the total concentration of malathion, parathion and methyl parathion is 20-200mg/m3And/or the concentration of chloral is 5-100mg/m3The degradation efficiency of malathion, parathion and methyl parathion can reach more than 90 percent, and the degradation efficiency of chloral can reach more than 75 percent;
The filler is one or more of polyurethane foam block, ceramsite, volcanic rock or molecular sieve, and the concentration of the bacterial strain LW-1 of the pseudomonas menbergii in the filler is 1.0 multiplied by 105-9.0×108CFU/g(Filler)。
the invention also provides a microbial active filler which is prepared by adsorbing the pseudomonas menhadiensis LW-1 bacterial suspension on the filler and filling the filler in the net surface hollow sphere, and can better treat and purify waste gas containing organic sulfur and/or organic chlorine.
The filler is one or more of polyurethane foam block, ceramsite, volcanic rock or molecular sieve;
The density of the filler is 20-80kg/m3the porosity is 50-97%, and the particle size is 10-100 mm;
the mass ratio of the microbial suspension to the filler is 1-12: 1;
the concentration of the bacterial body of the pseudomonas mendocina LW-1 in the microbial active filler is 1.0 multiplied by 105-9.0×108CFU/g(Filler)；
The net surface hollow spheres are made of polyethylene, polyvinyl chloride or polypropylene;
The diameter of the net surface hollow sphere is 6cm, the net surface hollow sphere is composed of two hemispheres, the surface of each hemisphere is porous, and the aperture of each hole is 2-8 mm;
The middle part of the net surface hollow ball is provided with a circle of reinforcing ring, and the reinforcing ring is provided with a lock catch which is convenient for opening and closing two hemispheres.
The preparation steps of the filler are as follows:
(1) Preparation of Pseudomonas monteilii LW-1 bacterial suspension
Activating an inclined plane of pseudomonas menbergii LW-1, inoculating the activated pseudomonas menbergii LW-1 into a liquid fermentation culture medium, and performing stepwise amplification fermentation culture according to 10-25% of inoculation amount;
The fermentation culture conditions are as follows: culturing at 35-45 deg.C under ventilation rate of 10-100L/min, dissolved oxygen of 2.0mg/L and pot pressure of 0.03-0.10Mpa for 45-55 h;
the liquid fermentation medium comprises the following components: glucose 0.5g, K2HPO4 0.5g，KH2PO4 1.5g，(NH4)2SO40.5g，NaCl 0.5g，MgSO4 0.2g，CaCl2 0.05g，FeSO40.02g, 1000mL of water, pH 6.2-7.0, sterilizing at 115 deg.C for 20min, cooling to room temperature, and mixing4.5mg/L of parathion and/or chloral (4.5 mg/L of parathion for treating sulfur-containing waste gas; 4.5mg/L of chloral for treating chlorine-containing waste gas; 4.5mg/L of parathion and 4.5mg/L of chloral for treating sulfur-containing and content-containing waste gas) are added;
Secondly, after the fermentation culture is finished, placing the fermentation liquor in a refrigerated centrifuge at 4 ℃, 2000-8000rpm, centrifuging for 10-20min, discarding the supernatant, diluting the precipitate with sterile nutrient solution to obtain a pseudomonas monteilii LW-1 bacterial suspension with the thallus concentration of 0.30-0.95 g/L;
The sterile nutrient solution comprises the following components: glucose 0.5g, K2HPO4 0.5g，KH2PO4 1.5g，(NH4)2SO40.5g，NaCl 0.5g，MgCl2 0.2g，CaCl2 0.05g，FeSO40.02g of water, 1000mL of water, 6.8-7.2 of pH value, and sterilizing for 20min at 121 ℃;
(2) preparation of the microbial active filler:
filling a filler in a square block or particle shape into the net surface hollow spheres, wherein the mass ratio of the filler to the net surface hollow spheres is 0.9-3: 1;
Secondly, spraying the pseudomonas menengei LW-1 bacterial suspension prepared in the step (1) onto the surface of a filler in a cubic or granular shape through holes of the mesh-surface hollow spheres;
Putting the net surface hollow balls loaded with the filler into a rotary mixer for rotary mixing;
Fourthly, spraying pseudomonas menbergii LW-1 bacterial suspension on the surface of the net surface hollow ball filled with the filler again;
repeating the steps for 2-3 times to ensure that the surface of the hollow sphere and the filler in the hole are loaded with bacterial cells, and the obtained pseudomonas mendocina LW-1 has the thallus concentration of 1.0 multiplied by 105-9.0×108CFU/g(Filler)The microbial active filler is stored at 4 ℃ for later use.
The invention also provides a method for treating waste gas containing organic sulfur and/or organic chlorine by using the microbial active filler, which comprises the following steps:
(1) Placing the microbial active filler loaded with the pseudomonas montmorii LW-1 into a container, introducing a parathion-containing gas (when sulfur-containing waste gas is treated), and activating the microbial active filler by taking parathion as a phosphorus source and a sulfur source; the activation time is 2-15 days;
the concentration of the parathion in the introduced parathion-containing gas is 50-200mg/m3；
when chlorine-containing waste gas is treated, introducing chloral-containing gas, and activating the microbial active filler by chloral; the activation time is 2-15 days; the concentration of the chloral is 50-200mg/m3；
When sulfur-containing and chlorine-containing waste gas is treated, introducing gas containing parathion and chloral to activate the microbial active filler; the activation time is 2-15 days; the concentration of parathion is 50-200mg/m3The concentration of chloral is 50-200mg/m3；
(2) placing the activated microbial active filler in a bioreactor, introducing waste gas containing organic sulfur and/or chlorine, purifying the organic sulfur and/or chlorine in the waste gas by using pseudomonas menbergii LW-1, wherein the volume of the microbial active filler is 0.1-1000m3The total concentration of organic sulfur is 20-250mg/m3And/or the total concentration of organic chlorine is 5-100mg/m3The gas flow rate is 0.05-10000m3H, the retention time is 1.5-10.0min, and the purification temperature is 30-65 ℃; regularly spraying nutrient solution on the surface of the microbial active filler to maintain the activity of the microbial active filler, wherein the spraying frequency is 1-3 days, the spraying speed is 1.0-200L/min each time, and the spraying time is 10-90min each time;
The nutrient solution comprises the following components: glucose 0.5g, K2HPO4 0.5g，KH2PO4 1.5g，(NH4)2SO4 0.5g，NaCl 0.5g，MgSO4 0.2g，CaCl2 0.05g，FeSO40.02g, 1000ml of water and the pH value of the mixture is 6.2-7.0.
Has the advantages that:
1. The Pseudomonas menhadiensis (Pseudomonas monteilii) LW-1 has higher efficiency of degrading organic sulfur and organic chlorine, the efficiency of degrading the organic sulfur can reach 90-100%, and the efficiency of degrading the organic chlorine can reach more than 75%; and the adaptation period of the dominant bacteria is short, the degradation reaction can be quickly started within 1 week after inoculation, and the start period can be shortened when the dominant bacteria is applied to a bioreactor for purifying waste gas containing organic sulfur.
2. By utilizing the effects of multiple mechanics such as sedimentation, adhesion, centrifugation and the like, the degraded Pseudomonas menhadenii (LW-1) is attached to the light porous material in the net surface hollow sphere to form the microbial active filler, and bacterial cells can be uniformly attached to the surface of the filler and can enter pores in the filler, so that the bacterial cell attachment amount of the microbial active filler is high, and the microbial active filler is not easy to lose from a bioreactor.
3. The Pseudomonas mondii (LW-1) has the characteristics of wide distribution, large quantity, simple nutrition requirement, quick propagation and strong competitive colonization ability. The pseudomonas has obvious advantages in iron competition by secreting antibiotics and inhibiting other microorganisms, contains siderophagins and can secrete siderophores.
4. pseudomonas mondii (LW-1) is chemotrophic, has a catalase, and can utilize and decompose volatile organosulfur/organochlorine.
5. Pseudomonas mondii (Pseudomonas monteilii) LW-1 has antiviral activity, has inhibitory effect on TMV (tobacco mosaic virus), PVY (potato virus Y) and other viruses, and is safe to use.
6. the surface of the carrier material for the microbial active filler is rough and porous, and is suitable for bacterial cell adhesion; the specific surface area is large, which is beneficial to the contact of bacterial cells with volatile organic sulfur and organic chlorine such as parathion, chloral and the like; the net surface hollow spheres filled with the light filler have a plurality of pores, good air permeability, small resistance and high mass transfer efficiency; the microbial active filler has light weight, high strength and small pressure loss; high temperature resistance and corrosion resistance; is not easy to rot and deform, and can be used for a long time and adapt to various complex practical conditions.
description of the drawings:
FIG. 1 Pseudomonas monteilii (Pseudomonas monteilii) LW-1 phylogenetic tree.
The specific implementation mode is as follows:
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present patent and are not intended to limit the present invention.
And (3) strain identification:
DNA extraction: extracting and purifying the screened strain DNA by using a full-automatic magnetic bead nucleic acid extractor Smart LabAssist-16 and a corresponding environment sample DNA extraction and purification kit (Taiwan round dot nanotechnology Co., Ltd.), and performing PCR amplification by using bacterial universal primers F16S-27 and R16S-1492, wherein the primer sequences are respectively as follows:
F16S-27(5`-AGAGTTTGATCCTGGCTCAG-3`)
R16S-1492(5`-CGGTTACCTTGTTACGACTTC-3`)
The PCR conditions were set as follows: pre-denaturation at 94 ℃ for 5 min; then denaturation at 94 ℃ for 30s, annealing at 56 ℃ for 30s, and extension at 72 ℃ for 90s, and circulating for 25 cycles; then extending for 10min at 72 ℃; finally, the temperature is kept at 4 ℃ for 10 min. After purifying and quality testing the PCR product, the PCR purified product is connected to a PMD-18T vector and then transformed into Escherichia coli DH5 alpha competent cells. 50. mu.L of the transformed competent cells were plated on LB medium containing isopropylthiogalactoside, X-gal and ampicillin. The culture was carried out overnight in a 37 ℃ incubator, and 25 white single plaques were picked from each plate and cultured in liquid LB medium. After 2 hours of incubation, the culture broth was subjected to sequencing analysis. The measured sequences are firstly subjected to similarity merging by adopting DNAMAN software, the sequences with the similarity of more than 97 percent are merged into an operation unit, a representative sequence in each operation unit is selected to perform homology analysis by utilizing BLAST and 16SrDNA gene sequences in a GenBank database, the length of more than 1400bp is selected to perform alignment (Cluselx 1.83), the adjacent position connection (Neighbourg Joining) method is adopted to perform systematic analysis (MEGA3.1), and a phylogenetic tree is shown in figure 1. After DNA sequence alignment, the strain is Pseudomonas mendelii (Pseudomonas monteilii) and is named as Pseudomonas mendelii (Pseudomonas monteilii) LW-1.
Example 1A microbial inoculum for purifying organic sulfur-containing waste gas
A microbial inoculum prepared from Pseudomonas monteilii LW-1 can be used for purifying waste gas containing organic sulfur and solving the pollution problem of volatile organic sulfur generated in the processes of pesticide wastewater treatment and the like, and the preparation method of the microbial inoculum comprises the following steps:
(1) Preparation method of fermentation liquor
Inoculating Pseudomonas mendii LW-1 into fermentation culture medium after slant culture, performing amplification culture step by step according to 10% inoculum concentration, and culturing at 35 deg.C, ventilation rate of 10L/min, dissolved oxygen of 2.0mg/L and tank pressure of 0.03-0.10Mpa for 55 hr to obtain fermentation liquid;
The slant culture medium comprises: glucose 0.5g, K2HPO4 0.5g，KH2PO4 1.5g，(NH4)2SO4 0.5g，NaCl 0.5g，MgSO4 0.2g，CaCl2 0.05g，FeSO40.02g, 1000ml of water, 20g of agar, 6.2-7.0 of pH value and 20min of sterilization at 115 ℃;
The fermentation medium is as follows: glucose 0.5g, K2HPO4 0.5g，KH2PO4 1.5g，(NH4)2SO4 0.5g，NaCl 0.5g，MgSO4 0.2g，CaCl2 0.05g，FeSO40.02g of water and 1000mL of water, the pH value is 6.2-7.0, the sterilization is carried out for 20min at the temperature of 115 ℃, and after the sterilization, 4.5mg/L of parathion is added after the cooling to the room temperature;
(2) Liquid microbial inoculum preparation method
Placing the fermentation liquor in a refrigerated centrifuge at 4 ℃, 2000rpm, centrifuging and concentrating for 20min, discarding supernatant, diluting the precipitate with sterile nutrient solution to obtain bacterial suspension with the thallus concentration of 0.3 g/L;
packaging the bacterial suspension into a liquid microbial inoculum to form a pseudomonas monteilii microbial inoculum product for treating volatile organic sulfur waste gas;
The sterile nutrient solution is as follows: glucose 0.5g, K2HPO4 0.5g，KH2PO4 1.5g，(NH4)2SO4 0.5g，NaCl 0.5g，MgCl2 0.2g，CaCl2 0.05g，FeSO40.02g, 1000mL of water, pH 6.2-7.0, sterilizing at 115 ℃ for 20 min.
The method for treating the waste gas containing organic sulfur by using the microbial inoculum comprises the following steps:
The liquid microbial inoculum is loaded on a polyurethane foam block filler, and the concentration of the pseudomonas monteilii LW-1 thallus in the filler is 1.0 multiplied by 105CFU/g(Filler)used for treating the organic sulfur-containing waste gas generated by a pesticide waste water storage pool, and the gas flow is 100m3the residence time is 1.0min, the temperature is 35 ℃, and the concentration of malathion is 13.2mg/m3The concentration of parathion is 33.7mg/m3The concentration of methyl parathion is 8.3mg/m3The malathion removal rate is 92.6%, the parathion degradation efficiency is 85.1%, and the methyl parathion degradation efficiency can reach 90.7%.
Example 2A microbial inoculum for purifying organic sulfur-containing waste gas
A microbial inoculum prepared from Pseudomonas monteilii LW-1 can be used for purifying waste gas containing organic sulfur and solving the pollution problem of volatile organic sulfur generated in the processes of pesticide wastewater treatment and the like, and the preparation method of the microbial inoculum comprises the following steps:
(1) preparation method of fermentation liquor
Inoculating Pseudomonas mendii LW-1 into fermentation culture medium after slant culture, performing amplification culture step by step according to 25% inoculum size, and culturing for 45h at 45 deg.C, ventilation rate of 100L/min, dissolved oxygen of 2.0mg/L, and tank pressure of 0.03-0.10Mpa to obtain fermentation liquid;
the slant culture medium comprises: glucose 0.5g, K2HPO4 0.5g，KH2PO4 1.5g，(NH4)2SO4 0.5g，NaCl 0.5g，MgSO4 0.2g，CaCl2 0.05g，FeSO40.02g, 1000ml of water, 20g of agar, 6.2-7.0 of pH value and 20min of sterilization at 115 ℃;
The fermentation medium is as follows: glucose 0.5g, K2HPO4 0.5g，KH2PO4 1.5g，(NH4)2SO4 0.5g，NaCl 0.5g，MgSO4 0.2g，CaCl2 0.05g，FeSO40.02g of water and 1000mL of water, the pH value is 6.2-7.0, the sterilization is carried out for 20min at the temperature of 115 ℃, and after the sterilization, 4.5mg/L of parathion is added after the cooling to the room temperature;
(2) liquid microbial inoculum preparation method
Placing the fermentation liquor in a refrigerated centrifuge at 4 deg.C and 8000rpm, centrifuging and concentrating for 5min, discarding supernatant, diluting the precipitate with sterile nutrient solution to obtain bacterial suspension with thallus concentration of 0.8 g/L;
Packaging the bacterial suspension into a liquid microbial inoculum to form a pseudomonas monteilii microbial inoculum product for treating volatile organic sulfur waste gas;
The sterile nutrient solution is as follows: glucose 0.5g, K2HPO4 0.5g，KH2PO4 1.5g，(NH4)2SO4 0.5g，NaCl 0.5g，MgCl2 0.2g，CaCl2 0.05g，FeSO40.02g, 1000mL of water, pH 6.2-7.0, sterilizing at 115 ℃ for 20 min.
the method for treating the waste gas containing organic sulfur by using the microbial inoculum comprises the following steps:
The liquid microbial inoculum is loaded on a ceramsite filler, and the concentration of the pseudomonas montmorii LW-1 thallus in the filler is 9.0 multiplied by 108CFU/g(Filler)Used for treating the organic sulfur-containing waste gas generated by a pesticide waste water storage pool, and the gas flow is 5000m3the residence time is 5.0min, the temperature is 55 ℃, and the total concentration of malathion, parathion and methyl parathion is 132.5mg/m3the degradation efficiency of malathion, parathion and methyl parathion can reach 91.4%.
Example 3A microbial inoculum for purifying organic sulfur-containing waste gas
A microbial inoculum prepared from Pseudomonas monteilii LW-1 can be used for purifying waste gas containing organic sulfur and solving the pollution problem of volatile organic sulfur generated in the processes of pesticide wastewater treatment and the like, and the preparation method of the microbial inoculum comprises the following steps:
(1) Preparation method of fermentation liquor
Inoculating pseudomonas menbergii LW-1 into a fermentation culture medium after slant culture, carrying out amplification culture step by step according to 18% of inoculum concentration, and carrying out fermentation conditions: culturing at 40 deg.C under ventilation rate of 50L/min, dissolved oxygen of 2.0mg/L and tank pressure of 0.03-0.10Mpa for 50h to obtain fermentation liquid;
the slant culture medium comprises: glucose 0.5g, K2HPO4 0.5g，KH2PO4 1.5g，(NH4)2SO4 0.5g，NaCl 0.5g，MgSO4 0.2g，CaCl2 0.05g，FeSO40.02g, 1000ml of water, 20g of agar, 6.2-7.0 of pH value and 20min of sterilization at 115 ℃;
The fermentation medium is as follows: glucose 0.5g, K2HPO4 0.5g，KH2PO4 1.5g，(NH4)2SO4 0.5g，NaCl 0.5g，MgSO4 0.2g，CaCl2 0.05g，FeSO40.02g of water and 1000mL of water, the pH value is 6.2-7.0, the sterilization is carried out for 20min at the temperature of 115 ℃, and after the sterilization, 4.5mg/L of parathion is added after the cooling to the room temperature;
(2) Liquid microbial inoculum preparation method
Placing the fermentation liquor in a refrigerated centrifuge at 4 ℃, 5000rpm, centrifuging and concentrating for 10min, discarding supernatant, diluting the precipitate with sterile nutrient solution to obtain bacterial suspension with the thallus concentration of 0.6 g/L;
Packaging the bacterial suspension into a liquid microbial inoculum to form a pseudomonas monteilii microbial inoculum product for treating volatile organic sulfur waste gas;
The sterile nutrient solution is as follows: glucose 0.5g, K2HPO4 0.5g，KH2PO4 1.5g，(NH4)2SO4 0.5g，NaCl 0.5g，MgCl2 0.2g，CaCl2 0.05g，FeSO40.02g, 1000mL of water, pH 6.2-7.0, sterilizing at 115 ℃ for 20 min.
The method for treating the waste gas containing organic sulfur by using the microbial inoculum comprises the following steps:
Loading the liquid microbial inoculum on volcanic rock filler, wherein the concentration of the pseudomonas monteilii LW-1 thallus in the filler is 5.0 multiplied by 107CFU/g(Filler)used for treating the organic sulfur-containing waste gas generated by a pesticide waste water storage pool, and the gas flow is 2500m3the residence time is 3.0min, the temperature is 45 ℃, and the concentrations of malathion, parathion and methyl parathion are respectively 16.3mg/m3，20.8mg/m3and 6.5mg/m3the degradation efficiency of malathion, parathion and methyl parathion can reach 89.2 percent, 93.6 percent and 92.5 percent respectively.
Example 1' an agent for purifying organic chlorine-containing waste gas
The preparation method of the microbial inoculum is the same as that of the example 1, and only the parathion in the fermentation culture medium in the step (1) is replaced by the same amount of chloral.
The method for treating the waste gas containing the organic chloride by using the microbial inoculum comprises the following specific steps:
The liquid microbial inoculum is loaded on a polyurethane foam block filler, and the concentration of the pseudomonas monteilii LW-1 thallus in the filler is 5.0 multiplied by 106CFU/g(Filler)Used for treating the organic chlorine-containing waste gas generated by a pesticide waste water storage tank, and the gas flow is 100m3H, residence time of 1.0min, temperature of 35 ℃, chloral concentration of 42.6mg/m3The degrading efficiency of the chloral can reach 80.3 percent.
Example 2' an agent for purifying organic chlorine-containing waste gas
The preparation method of the microbial inoculum is the same as that in example 2, only the parathion in the fermentation culture medium in the step (1) is replaced by the same amount of chloral.
The method for treating the waste gas containing the organic chloride by using the microbial inoculum comprises the following specific steps:
The liquid microbial inoculum is loaded on a ceramsite filler, and the concentration of the pseudomonas montmorii LW-1 thallus in the filler is 9.0 multiplied by 108CFU/g(Filler)Is used for treating the organic chlorine-containing waste gas generated by a pesticide waste water storage tank, and has the gas flow of 5000m3H, retention time of 5.0min, temperature of 55 ℃, chloral concentration of 20.2mg/m3The concentration of chloral after treatment is reduced to 3.6mg/m3The degradation efficiency can reach 82.2%.
Example 3' an agent for purifying organic chlorine-containing waste gas
the preparation method of the microbial inoculum is the same as that in example 3, only the parathion in the fermentation medium in the step (1) is replaced by the same amount of chloral.
The method for treating the waste gas containing the organic chloride by using the microbial inoculum comprises the following specific steps:
Loading the liquid microbial inoculum on volcanic rock filler, wherein the concentration of the pseudomonas monteilii LW-1 thallus in the filler is 4.0 multiplied by 108CFU/g(Filler)Organic fertilizer for treating pesticide waste water produced in reservoirchlorine waste gas with a gas flow of 2500m3H, residence time of 3.0min, temperature of 45 ℃, chloral concentration of 61.9mg/m3the degrading efficiency of the chloral can reach 76.5 percent.
Example 4: microbial filler for removing organic sulfur-containing waste gas and preparation thereof
A microbial active filler is prepared by adsorbing Pseudomonas mendii LW-1 bacterial suspension on a polyurethane foam block filler and filling the filler in a net surface hollow sphere;
The filler density is 20kg/m3the porosity is 90%, and the particle size is 10 mm; the mass ratio of the microbial suspension to the filler is 1: 1; the thallus concentration of the pseudomonas monteilii LW-1 in the microbial active filler is 1.0 multiplied by 105CFU/g(Filler)；
The net surface hollow spheres are made of polyethylene; the diameter of the net surface hollow ball is 6cm, the net surface hollow ball is composed of two hemispheres, the surface of each hemisphere is porous, and the aperture of each hole is 2 mm; there is the round reinforcing ring at net face clean shot middle part, is equipped with the hasp on the reinforcing ring, is convenient for open and two closed hemispheres.
The preparation steps of the filler are as follows:
(1) Preparation of Pseudomonas monteilii LW-1 bacterial suspension
Activating an inclined plane of pseudomonas menbergii LW-1, inoculating the activated pseudomonas menbergii LW-1 into a liquid fermentation culture medium, and performing stepwise amplification fermentation culture according to 10% inoculation quantity;
The fermentation culture conditions are as follows: culturing at 35 deg.C under ventilation rate of 10L/min, dissolved oxygen of 2.0mg/L and pot pressure of 0.03-0.10Mpa for 55 hr;
The slant culture medium comprises: glucose 0.5g, K2HPO4 0.5g，KH2PO4 1.5g，(NH4)2SO4 0.5g，NaCl 0.5g，MgSO4 0.2g，CaCl2 0.05g，FeSO40.02g, 1000ml of water, 20g of agar, 6.2-7.0 of pH value and 20min of sterilization at 115 ℃;
The liquid fermentation medium comprises the following components: glucose 0.5g, K2HPO4 0.5g，KH2PO4 1.5g，(NH4)2SO40.5g，NaCl 0.5g，MgSO4 0.2g，CaCl2 0.05g，FeSO40.02g of water and 1000mL of water, the pH value is 6.2-7.0, the sterilization is carried out for 20min at the temperature of 115 ℃, and after the sterilization, 4.5mg/L of parathion is added after the cooling to the room temperature;
Secondly, after the fermentation culture is finished, placing the fermentation liquor in a refrigerated centrifuge at 4 ℃, 2000rpm, centrifuging for 10min, removing supernatant, diluting the precipitate with sterile nutrient solution, and obtaining pseudomonas mundtii LW-1 bacterial suspension with the thallus concentration of 0.30 g/L;
the sterile nutrient solution comprises the following components: glucose 0.5g, K2HPO4 0.5g，KH2PO4 1.5g，(NH4)2SO40.5g，NaCl 0.5g，MgCl2 0.2g，CaCl2 0.05g，FeSO40.02g of water, 1000mL of water, 6.8-7.2 of pH value, and sterilizing for 20min at 121 ℃;
(2) preparation of the microbial active filler:
Filling a square block filler into the net surface hollow spheres, wherein the mass ratio of the filler to the net surface hollow spheres is 0.9: 1;
Secondly, spraying the pseudomonas menengei LW-1 bacterial suspension prepared in the step (1) onto the surface of the filler in the shape of a square block through the holes of the mesh surface hollow spheres;
Putting the net surface hollow balls loaded with the filler into a rotary mixer for rotary mixing;
fourthly, spraying pseudomonas menbergii LW-1 bacterial suspension on the surface of the net surface hollow ball filled with the filler again;
Repeating the steps for 2 times to ensure that the surfaces of the hollow spheres and the fillers in the holes are loaded with bacterial cells, and the obtained pseudomonas mendocina LW-1 has the thallus concentration of 1.0 multiplied by 105CFU/g(Filler)The microbial active filler is stored at 4 ℃ for later use.
Example 5: microbial filler for removing organic sulfur-containing waste gas and preparation thereof
A microbial active filler is prepared by adsorbing Pseudomonas mendii LW-1 bacterial suspension on a ceramsite filler and filling the filler into a mesh surface hollow sphere; the filler density is 80kg/m3the porosity is 50%, and the particle size is 100 mm; microbial suspension and fillerThe mass ratio of (A) to (B) is 12: 1; the thallus concentration of the pseudomonas monteilii LW-1 in the microbial active filler is 9.0 multiplied by 108CFU/g(Filler)；
The net surface hollow spheres are made of polyvinyl chloride; the diameter of the net surface hollow ball is 6cm, the net surface hollow ball is composed of two hemispheres, the surface of each hemisphere is porous, and the aperture of each hole is 8 mm; there is the round reinforcing ring at net face clean shot middle part, is equipped with the hasp on the reinforcing ring, is convenient for open and two closed hemispheres.
The preparation steps of the filler are as follows:
(1) Preparation of Pseudomonas monteilii LW-1 bacterial suspension
Activating an inclined plane of pseudomonas menbergii LW-1, inoculating the activated pseudomonas menbergii LW-1 into a liquid fermentation culture medium, and performing stepwise amplification fermentation culture according to 25% inoculation quantity;
the fermentation culture conditions are as follows: culturing at 45 deg.C under ventilation rate of 100L/min, dissolved oxygen of 2.0mg/L and tank pressure of 0.10Mpa for 45 hr;
the slant culture medium comprises: glucose 0.5g, K2HPO4 0.5g，KH2PO4 1.5g，(NH4)2SO4 0.5g，NaCl 0.5g，MgSO4 0.2g，CaCl2 0.05g，FeSO40.02g, 1000ml of water, 20g of agar, 6.2-7.0 of pH value and 20min of sterilization at 115 ℃;
The liquid fermentation medium comprises the following components: glucose 0.5g, K2HPO4 0.5g，KH2PO4 1.5g，(NH4)2SO40.5g，NaCl 0.5g，MgSO4 0.2g，CaCl2 0.05g，FeSO40.02g of water and 1000mL of water, the pH value is 6.2-7.0, the sterilization is carried out for 20min at the temperature of 115 ℃, and after the sterilization, 4.5mg/L of parathion is added after the cooling to the room temperature;
secondly, after the fermentation culture is finished, placing the fermentation liquor in a refrigerated centrifuge, centrifuging for 20min at the temperature of 4 ℃ and the rpm of 8000, discarding supernatant, diluting the precipitate with sterile nutrient solution, and obtaining pseudomonas mundtii LW-1 bacterial suspension with the thallus concentration of 0.95 g/L;
The sterile nutrient solution comprises the following components: glucose 0.5g, K2HPO4 0.5g，KH2PO4 1.5g，(NH4)2SO40.5g，NaCl 0.5g，MgCl2 0.2g，CaCl2 0.05g，FeSO40.02g of water, 1000mL of water, 6.8-7.2 of pH value, and sterilizing for 20min at 121 ℃;
(2) Preparation of the microbial active filler:
filling a granular filler into the net surface hollow spheres, wherein the mass ratio of the filler to the net surface hollow spheres is 2: 1;
Secondly, spraying the pseudomonas menenginensis LW-1 bacterial suspension prepared in the step (1) onto the surface of the filler in a particle shape through the holes of the mesh surface hollow spheres;
putting the net surface hollow balls loaded with the filler into a rotary mixer for rotary mixing;
fourthly, spraying pseudomonas menbergii LW-1 bacterial suspension on the surface of the net surface hollow ball filled with the filler again;
repeating the steps for 3 times to ensure that the surfaces of the hollow spheres and the fillers in the holes are loaded with bacterial cells, and the obtained pseudomonas mendocina LW-1 has the thallus concentration of 9.0 multiplied by 108CFU/g(Filler)The microbial active filler is stored at 4 ℃ for later use.
Example 6: microbial filler for removing organic sulfur-containing waste gas and preparation thereof
A microbial active filler is prepared by adsorbing Pseudomonas monteilii LW-1 bacterial suspension on volcanic rock filler and filling the filler in a net surface hollow sphere; the filler density is 50kg/m3The porosity is 75 percent, and the particle size is 50 mm; the mass ratio of the microbial suspension to the filler is 8: 1; the thallus concentration of the pseudomonas mendii LW-1 in the microbial active filler is 3.0 multiplied by 107CFU/g(Filler)；
The net surface hollow spheres are made of polypropylene; the diameter of the net surface hollow ball is 6cm, the net surface hollow ball is composed of two hemispheres, the surface of each hemisphere is porous, and the aperture of each hole is 5 mm; there is the round reinforcing ring at net face clean shot middle part, is equipped with the hasp on the reinforcing ring, is convenient for open and two closed hemispheres.
The preparation steps of the filler are as follows:
(1) Preparation of Pseudomonas monteilii LW-1 bacterial suspension
Activating an inclined plane of pseudomonas menbergii LW-1, inoculating the activated pseudomonas menbergii LW-1 into a liquid fermentation culture medium, and performing stepwise amplification fermentation culture according to 17% inoculation quantity;
The fermentation culture conditions are as follows: culturing at 40 deg.C under ventilation rate of 50L/min, dissolved oxygen of 2.0mg/L and pot pressure of 0.03-0.10Mpa for 50 h;
The slant culture medium comprises: glucose 0.5g, K2HPO4 0.5g，KH2PO4 1.5g，(NH4)2SO4 0.5g，NaCl 0.5g，MgSO4 0.2g，CaCl2 0.05g，FeSO40.02g, 1000ml of water, 20g of agar, 6.2-7.0 of pH value and 20min of sterilization at 115 ℃;
The liquid fermentation medium comprises the following components: glucose 0.5g, K2HPO4 0.5g，KH2PO4 1.5g，(NH4)2SO40.5g，NaCl 0.5g，MgSO4 0.2g，CaCl2 0.05g，FeSO40.02g of water and 1000mL of water, the pH value is 6.2-7.0, the sterilization is carried out for 20min at the temperature of 115 ℃, and after the sterilization, 4.5mg/L of parathion is added after the cooling to the room temperature;
secondly, after the fermentation culture is finished, placing the fermentation liquor in a refrigerated centrifuge at 4 ℃, 5000rpm, centrifuging for 10-20min, discarding supernatant, diluting the precipitate with sterile nutrient solution to obtain pseudomonas mundtii LW-1 bacterial suspension with the thallus concentration of 0.65 g/L;
the sterile nutrient solution comprises the following components: glucose 0.5g, K2HPO4 0.5g，KH2PO4 1.5g，(NH4)2SO40.5g，NaCl 0.5g，MgCl2 0.2g，CaCl2 0.05g，FeSO40.02g of water, 1000mL of water, 6.8-7.2 of pH value, and sterilizing for 20min at 121 ℃;
(2) preparation of the microbial active filler:
filling a square block filler into the net surface hollow spheres, wherein the mass ratio of the filler to the net surface hollow spheres is 3: 1;
Secondly, spraying the pseudomonas menengei LW-1 bacterial suspension prepared in the step (1) onto the surface of the square filler through the holes of the mesh surface hollow spheres;
Putting the net surface hollow balls loaded with the filler into a rotary mixer for rotary mixing;
Fourthly, spraying pseudomonas menbergii LW-1 bacterial suspension on the surface of the net surface hollow ball filled with the filler again;
repeating the steps for 3 times to ensure that the surfaces of the hollow spheres and the fillers in the holes are loaded with bacterial cells, and the obtained pseudomonas mendocina LW-1 has the thallus concentration of 3.0 multiplied by 107CFU/g(Filler material)The microbial active filler is stored at 4 ℃ for later use.
example 4' a microbial Filler for removing organic chlorine-containing waste gas and preparation thereof
The filler and the preparation method are the same as example 4, only the parathion in the preparation step (1) is replaced by the same amount of chloral.
EXAMPLE 5' A microbial Filler for removing organic chlorine-containing waste gas and preparation thereof
The filler and the preparation method are the same as example 5, only the parathion in the preparation step (1) is replaced by the same amount of chloral.
EXAMPLE 6' A microbial Filler for removing organic chlorine-containing waste gas and preparation thereof
The filler and the preparation method were the same as in example 6 except that the parathion in the preparation step (1) was replaced with an equal amount of chloral.
Example 7: method for treating organic sulfur-containing waste gas by using microbial active filler
The invention also provides a method for treating the organic sulfur-containing waste gas by using the microbial active filler in the embodiment 4, which comprises the following specific steps:
(1) The microbial active filler loaded with pseudomonas montmorii LW-1 in the embodiment 4 is placed in a container, gas containing parathion is introduced, and the parathion is taken as a phosphorus source and a sulfur source to activate the microbial active filler; the activation time is 15 days;
the concentration of the parathion in the introduced parathion-containing gas is 50mg/m3；
(2) The activated microbial active filler is placed in a bioreactor, organic sulfur-containing waste gas is introduced, and pseudomonas monteilii LW-1 is used for purifying the organic sulfur and the microbial activity in the waste gasThe volume of the filler is 0.1m3the concentration of malathion and parathion in the intake air is respectively 17.3mg/m3And 46.7mg/m3Gas flow rate of 0.05m3H, the retention time is 1.5min, and the purification temperature is 30 ℃; regularly spraying nutrient solution on the surface of the microbial active filler to maintain the activity of the microbial active filler, wherein the spraying frequency is 3 days, the spraying speed is 1.0L/min each time, and the spraying time is 10min each time; the concentration of malathion and parathion at the air outlet is reduced to 2.2mg/m3And 4.3mg/m3The removal rate reaches 87.3 percent and 90.8 percent respectively.
The nutrient solution comprises the following components: glucose 0.5g, K2HPO4 0.5g，KH2PO4 1.5g，(NH4)2SO4 0.5g，NaCl 0.5g，MgSO4 0.2g，CaCl2 0.05g，FeSO40.02g, 1000ml of water and the pH value of the mixture is 6.2-7.0.
Example 8: method for treating organic sulfur-containing waste gas by using microbial active filler
The invention also provides a method for treating the organic sulfur-containing waste gas by using the microbial active filler in the embodiment 5, which comprises the following specific steps:
(1) Placing the microbial active filler loaded with pseudomonas montmorii LW-1 obtained in the example 5 into a container, introducing gas containing parathion, and activating the microbial active filler by taking the parathion as a phosphorus source and a sulfur source; the activation time is 2 days;
The concentration of the parathion in the introduced parathion-containing gas is 200mg/m3；
(2) the activated microbial active filler is placed in a bioreactor, organic sulfur-containing waste gas is introduced, the organic sulfur in the waste gas is purified by pseudomonas menbergii LW-1, and the volume of the microbial active filler is 1000m3The concentration of parathion in the intake air is 162.7mg/m3The concentration of methyl parathion is 32.1mg/m3gas flow velocity of 10000m3H, the retention time is 10.0min, and the purification temperature is 65 ℃; regularly spraying nutrient solution on the surface of the microbial active filler to maintain the activity of the microbial active filler, wherein the spraying frequency is 1 spraying time for 1 day, and the spraying speed is every time200L/min, and each spraying time is 90 min; the concentration of parathion at the gas outlet is reduced to 11.3mg/m3The concentration of methyl parathion is reduced to 2.8mg/m3. The removal rates of the compounds reach 93.1 percent (parathion) and 91.3 percent (methyl parathion) respectively.
The nutrient solution comprises the following components: glucose 0.5g, K2HPO4 0.5g，KH2PO4 1.5g，(NH4)2SO4 0.5g，NaCl 0.5g，MgSO4 0.2g，CaCl2 0.05g，FeSO40.02g, 1000ml of water and the pH value of the mixture is 6.2-7.0.
Example 9: method for treating organic sulfur-containing waste gas by using microbial active filler
the invention also provides a method for treating the organic sulfur-containing waste gas by using the microbial active filler in the embodiment 6, which comprises the following specific steps:
(1) Placing the microbial active filler loaded with pseudomonas montmorii LW-1 obtained in the example 6 into a container, introducing gas containing parathion, and activating the microbial active filler by taking the parathion as a phosphorus source and a sulfur source; the activation time is 7 days;
the concentration of the parathion in the introduced parathion-containing gas is 100mg/m3；
(2) The activated microbial active filler is placed in a bioreactor, organic sulfur-containing waste gas is introduced, the organic sulfur in the waste gas is purified by pseudomonas menbergii LW-1, and the volume of the microbial active filler is 500m3the concentration of malathion in the intake air was 179.2mg/m3the concentration of methyl parathion is 56.3mg/m3gas flow rate of 5000m3h, the retention time is 5.0min, and the purification temperature is 50 ℃; regularly spraying nutrient solution on the surface of the microbial active filler to maintain the activity of the microbial active filler, wherein the spraying frequency is 2 days, the spraying frequency is 1 time, each time the spraying speed is 100L/min, each time the spraying time is 50min, an air outlet is formed, and the concentration of malathion is reduced to 13.9mg/m3The concentration of methyl parathion is reduced to 4.1mg/m3. The removal rates of malathion and methyl parathion respectively reach 92.2 percent and 92.7 percent.
The nutrient solution is prepared fromComprises the following components: glucose 0.5g, K2HPO4 0.5g，KH2PO4 1.5g，(NH4)2SO4 0.5g，NaCl 0.5g，MgSO4 0.2g，CaCl2 0.05g，FeSO40.02g, 1000ml of water and the pH value of the mixture is 6.2-7.0.
Example 7' method for treating waste gas containing organochlorine with a microbiologically active filler
The invention also provides a method for treating waste gas containing organic chlorine by using the microbial active filler described in the embodiment 4', the specific steps are the same as the embodiment 7, only the gas used for activating the microbial active filler in the step (1) is replaced by parathion-containing gas into chloral-containing gas, and the concentration is unchanged; replacing the waste gas containing organic sulfur introduced in the step (2) with the waste gas with the concentration of 37.4mg/m3Chloral off-gas.
The concentration of chloral is reduced to 6.2mg/m at the air outlet3the removing rate of the chloral reaches 83.4 percent.
Example 8' a method for treating an organic chlorine-containing waste gas with a microbiologically active filler
the invention also provides a method for treating waste gas containing organic chlorine by using the microbial active filler described in the embodiment 5', the specific steps are the same as the embodiment 8, only the gas used for activating the microbial active filler in the step (1) is replaced by parathion-containing gas into chloral-containing gas, and the concentration is unchanged; replacing the waste gas containing organic sulfur introduced in the step (2) with the waste gas with the concentration of 97.6mg/m3Chloral off-gas.
the concentration of chloral is reduced to 21.3mg/m at the air outlet3The removing rate of chloral reaches 78.2%.
Example 9' A method for treating an organic chlorine-containing waste gas with a microbiologically active filler
the invention also provides a method for treating waste gas containing organic chlorine by using the microbial active filler described in the embodiment 6', the specific steps are the same as the embodiment 9, only the gas used for activating the microbial active filler in the step (1) is replaced by parathion-containing gas into chloral-containing gas, and the concentration is unchanged; replacing the waste gas containing organic sulfur introduced in the step (2) with waste gas containing organic sulfurThe concentration is 13.5mg/m3chloral off-gas. The concentration of chloral is reduced to 2.7mg/m at the air outlet3the removing rate of the chloral reaches 80 percent.
Example 10: effect of the experiment
The filler obtained in example 6 was used for the treatment of the following gases:
(1) Treating waste gas containing parathion with gas flow rate of 300m3The residence time is 100s, the purification temperature is 30 ℃. The bioreactor continuously runs for 2 months, the pH value is 4-7, and the average intake concentration of parathion is as follows: 25mg/m3the concentration of the discharged gas is 2mg/m3and the removal rate reaches 92 percent.
the results of the comparative experiments show that: when no iron element is added into the nutrient solution, the number of the pseudomonas menbergii LW-1 is 3.0 multiplied by 10 after the reactor runs for 2 months7CFU/g increased to 7.0X 108CFU/g. When nutrient solution containing 0.02g/L of iron element is added into the bioreactor and the reactor runs for 2 months, the quantity of the pseudomonas menbergii LW-1 is controlled to be 3.0 multiplied by 107CFU/g increased to 1.0X 109CFU/g is increased remarkably, so that the bioreactor can effectively remove organic sulfur in the waste gas.
The nutrient solution comprises the following components: 0.5g of glucose, 0.5g of K2HPO40.5g, KH2PO41.5g, (NH4)2 SO40.5g, 0.5g of NaCl, 40.2g of MgSO40.2g, 20.05g of CaCl, 40.02g of FeSO40, 1000ml of water and the pH value of the mixture is 6.2-7.0; the spraying frequency is 2 days, the spraying is carried out for 1 time, the spraying speed is 200L/min each time, and the spraying time is 50min each time.
(2) used for treating malathion-containing waste gas generated in the pesticide wastewater treatment process, wherein the concentration of the malathion is 10mg/m3Gas flow rate of 3000m3The residence time is 95s, the gas temperature is 30 ℃, nutrient solution containing 0.02g/L of iron element is periodically supplemented (the same as above), and the removal rate of the malathion is close to 100 percent.
The equipment is operated for more than 3 months, the pH is 4-6, the amount of Pseudomonas mendocii (Pseudomonas monteilii) LW-1 is maintained at 2.0X 107-2.5×108CFU/g, the purification purpose is achieved, and meanwhile, the equipment can stably run for a long time.
(3) treating methyl mercaptan-containing waste gas with a gas flow of10L/min, a retention time of 90s and operation at 30 ℃. The bioreactor continuously runs for 10 months, the pH is 4-6, and the intake concentration of methyl mercaptan is as follows: 2-5mg/m3the concentration of the discharged gas is lower than 0.1mg/m3Average removal rate: 98 percent. The amount of Pseudomonas montii LW-1 in the reactor was 2.0X 107-2.5×108CFU/g, the bioreactor runs stably.
In the gas treatment process, the start-up period of the bioreactor is one week. Under the same conditions, after replacing the strains in the filler with activated sludge for treating pesticide wastewater, the start-up period is 2 weeks to 1 month.
Example 10': effect of the experiment
The packing obtained in example 6' was used for the treatment of the following gases:
Treating waste gas containing chloral with gas flow rate of 300m3the residence time is 100s, the purification temperature is 30 ℃. The bioreactor continuously runs for 8 months, the pH value is 4-6, and the average intake concentration of chloral is as follows: 30mg/m3the concentration of the discharged gas is 4.5mg/m3And the removal rate reaches 85 percent.
The results of the comparative experiments show that: when no iron element is added into the nutrient solution, the number of the pseudomonas menbergii LW-1 is 3.0 multiplied by 10 after the reactor runs for 1 month7CFU/g increased to 7.0X 107CFU/g. When nutrient solution containing 0.02g/L of iron element is added into the bioreactor and the reactor runs for 2 months, the quantity of the pseudomonas menbergii LW-1 is controlled to be 3.0 multiplied by 107CFU/g increased to 3.0X 108CFU/g is increased remarkably, so that the bioreactor can effectively remove organic chlorine in the waste gas for a long time.
The nutrient solution comprises the following components: 0.5g of glucose, 0.5g of K2HPO40.5g, KH2PO41.5g, (NH4)2 SO40.5g, 0.5g of NaCl, 40.2g of MgSO40.2g, 20.05g of CaCl, 40.02g of FeSO40, 1000ml of water and the pH value of the mixture is 6.2-7.0; the spraying frequency is 2 days, the spraying is carried out for 1 time, the spraying speed is 200L/min each time, and the spraying time is 50min each time.
(2) is used for treating waste gas containing chloral generated in the pesticide wastewater treatment process, and the concentration of the chloral is 10mg/m3gas flow rate of 3000m3H, residence time 95s, gasThe body temperature is 30 ℃, nutrient solution containing 0.02g/L of iron element is supplemented periodically (the same as above), the removal rate of chloral is 90 percent, and the discharged gas reaches the air pollutant discharge standard established by the state.
The equipment is operated for more than 3 months, the pH is 4-6, the amount of Pseudomonas menteilii (Pseudomonas monteilii) LW-1 is maintained at 7.0X 106-7.5×107CFU/g, the purification purpose is achieved, and meanwhile, the equipment can stably run for a long time.
In the gas treatment process, the start-up period of the bioreactor is one week. Under the same conditions, after replacing the strains in the filler with activated sludge for treating pesticide wastewater, the start-up period is 2 weeks to 1 month.
EXAMPLE 11 Mixed gas treatment
A method for treating waste gas containing organic sulfur and chlorine by using a microbial active filler prepared in the same manner as in example 6 (only 4.5mg/L of parathion is added to a fermentation culture medium and 4.5mg/L of parathion and 4.5mg/L of chloral are added at the same time) comprises the following specific steps:
(1) The microbial active filler loaded with pseudomonas montmorii LW-1 obtained in the example 6 is placed in a container, and gas containing parathion and chloral is introduced to activate the microbial active filler; the activation time is 7 days;
The concentration of the parathion in the introduced parathion-containing gas is 100mg/m3The concentration of chloral is 100mg/m3；
(2) the activated microbial active filler is placed in a bioreactor, waste gas containing organic sulfur and chlorine is introduced, the organic sulfur and chlorine in the waste gas are purified by pseudomonas menbergii LW-1, and the volume of the microbial active filler is 500m3In the intake air, malathion 37.7mg/m3Parathion 16.1mg/m3Chloral 9.8mg/m3(ii) a Gas flow rate of 5000m3h, the retention time is 5.0min, and the purification temperature is 50 ℃; regularly spraying nutrient solution on the surface of the microbial active filler to maintain the activity of the microbial active filler, wherein the spraying frequency is 2 days, the spraying frequency is 1 time, each time, the spraying speed is 100L/min, each time, the spraying time is 50min, an air outlet is formed, and malathion is 3.2mg/m3Parathion 1.3mg/m3Trichloro ethylaldehyde 2.3mg/m3. The Malathiophos removal rate is 91.5 percent, and the sulfur phosphorus removal rate is 92 percent and the chloral removal rate is 76.5 percent. .
The nutrient solution comprises the following components: glucose 0.5g, K2HPO4 0.5g，KH2PO4 1.5g，(NH4)2SO4 0.5g，NaCl 0.5g，MgSO4 0.2g，CaCl2 0.05g，FeSO40.02g, 1000ml of water and the pH value of the mixture is 6.2-7.0.
Claims (2)
1. The pseudomonas menbradii is characterized in that the pseudomonas menbradii is specifically pseudomonas menbradii (pseudomonas menbradii)Pseudomonas monteilii) LW-1, preservation number is CGMCC No. 13748.
2. The use of Pseudomonas mendii of claim 1 in the field of environmental remediation.
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Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1218421A (en) * | 1996-05-10 | 1999-06-02 | 帕克斯生物***公司 | Purification of gases containing hydrogen sulphide |
| CN1329138A (en) * | 2001-08-07 | 2002-01-02 | 中国科学院武汉病毒研究所 | Organophosphorus pesticide degradation bacterium and its preparation method |
| CN101096644A (en) * | 2006-06-26 | 2008-01-02 | 谢明 | Pseudomonas stutzeri JSD-008 and its degradation function for organophosphorus pesticide |
| CN103923893A (en) * | 2014-04-24 | 2014-07-16 | 华东理工大学 | Organic phospholipid hydrolase, gene, recombinant expression transformant and application thereof |
| CN103937576A (en) * | 2014-05-02 | 2014-07-23 | 中南大学 | Efficient biological desulfurization method for coal |
| CN104152207A (en) * | 2014-05-24 | 2014-11-19 | 北京大学工学院包头研究院 | Microbial desulfurization method of sulfide mineral |
| CN105002127A (en) * | 2015-08-24 | 2015-10-28 | 周俊冉 | Composite bacterium and application thereof to treatment of garlic processing wastewater |
| CN106398799A (en) * | 2016-10-24 | 2017-02-15 | 马鞍山科宇环境工程有限公司 | Method for biologically desulfurizing coal for power plants |
| CN106433855A (en) * | 2016-10-28 | 2017-02-22 | 中南大学 | Efficient coal desulfurization method by flotation and biological coupling |
| CN106906170A (en) * | 2017-05-03 | 2017-06-30 | 上田环境修复股份有限公司 | Complex micro organism fungicide and its preparation method and application |
| CN107699498A (en) * | 2017-10-04 | 2018-02-16 | 李萍 | A kind of complex microorganism preparations of degrading organic phosphor pesticides and preparation method thereof |
-
2018
- 2018-05-14 CN CN201810455504.0A patent/CN108342346B/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1218421A (en) * | 1996-05-10 | 1999-06-02 | 帕克斯生物***公司 | Purification of gases containing hydrogen sulphide |
| CN1329138A (en) * | 2001-08-07 | 2002-01-02 | 中国科学院武汉病毒研究所 | Organophosphorus pesticide degradation bacterium and its preparation method |
| CN101096644A (en) * | 2006-06-26 | 2008-01-02 | 谢明 | Pseudomonas stutzeri JSD-008 and its degradation function for organophosphorus pesticide |
| CN103923893A (en) * | 2014-04-24 | 2014-07-16 | 华东理工大学 | Organic phospholipid hydrolase, gene, recombinant expression transformant and application thereof |
| CN103937576A (en) * | 2014-05-02 | 2014-07-23 | 中南大学 | Efficient biological desulfurization method for coal |
| CN104152207A (en) * | 2014-05-24 | 2014-11-19 | 北京大学工学院包头研究院 | Microbial desulfurization method of sulfide mineral |
| CN105002127A (en) * | 2015-08-24 | 2015-10-28 | 周俊冉 | Composite bacterium and application thereof to treatment of garlic processing wastewater |
| CN106398799A (en) * | 2016-10-24 | 2017-02-15 | 马鞍山科宇环境工程有限公司 | Method for biologically desulfurizing coal for power plants |
| CN106433855A (en) * | 2016-10-28 | 2017-02-22 | 中南大学 | Efficient coal desulfurization method by flotation and biological coupling |
| CN106906170A (en) * | 2017-05-03 | 2017-06-30 | 上田环境修复股份有限公司 | Complex micro organism fungicide and its preparation method and application |
| CN107699498A (en) * | 2017-10-04 | 2018-02-16 | 李萍 | A kind of complex microorganism preparations of degrading organic phosphor pesticides and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| （Cloning and expression of the phosphotriesterase gene hocA from Pseudomonas monteilii C11;Horne I 等;《Microbiology》;20020901;第148卷(第9期);第2687-2695页 * |
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