CN108330150A - A kind of oligofructose prepared as raw material using brown sugar - Google Patents
A kind of oligofructose prepared as raw material using brown sugar Download PDFInfo
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Abstract
The invention belongs to oligofructose synthesis technical fields, and in particular to a kind of oligofructose prepared as raw material using brown sugar is prepared by following methods：(1) immobilized fructosyltransferase is added in brown sugar solution and carries out conversion reaction, obtain syrup；(2) it is separated by filtration syrup；(3) after the syrup concentrated sterilization after being separated by filtration to obtain the final product.The present invention is converted by immobilized fructosyltransferase, is separated by filtration, concentrated sterilization step, oligofructose is made in brown sugar using brown sugar as raw material.The oligofructose prepared as raw material using brown sugar, it can promote the proliferation of Bifidobacterium in enteron aisle, inhibit enterobacteria, enterococcal proliferation, has the function of adjusting intestinal flora, and it inhibits enterobacteria and enterococcal effect to be substantially better than the oligofructose in white granulated sugar source.
Description
Technical field
The invention belongs to oligofructose synthesis technical fields, and in particular to a kind of oligomeric fruit prepared as raw material using brown sugar
Sugar.
Background technology
Oligofructose (Fructooligosaccharides, FOS), also known as Fructooligosaccharides, sugarcane fructooligosaccharide, fructo-oligose etc.,
It is naturally occurring in many plants of daily consumption, such as onion, banana, garlic, wheat, barley, asparagus, pea, Jerusalem artichoke, bee
Honey etc..Oligofructose is the functional oligose that the degree of polymerization is 2~9.By structure, oligofructose can be divided into sugarcane-fruit type and fruit-fruit
Two kinds of type.
Since oligofructose is not digested absorption, thus large intestine can be reached and utilized for Bifidobacterium, be having for Bifidobacterium
Imitate proliferation factor.The organic acid that oligofructose generates after being utilized by Bifidobacterium can be such that intestinal pH declines, and inhibit husky in enteron aisle
The generation of the spoilage organisms such as door bacterium, improves intestinal environment, reduces the generation of enteral spoilage product, flat to adjust intestinal microecology
Weighing apparatus maintains intestinal health.
In existing commercialization oligofructose product, oligofructose is made using witloof or white granulated sugar of raw material.It is prepared
Liquid oligomers fructose be colourless or pale yellow transparent thick liquid, there is distinctive fragrance, sweet taste is soft salubrious, free from extraneous odour,
Without twenty-twenty vision visible foreign.Solid oligofructose is white or yellowish, has peculiar fragrance, sweet taste is soft salubrious, is no different
Taste is visible by naked eyes impurity.
Currently, the raw material used in industrial production oligofructose is jerusalem artichoke, witloof or white granulated sugar.Using jerusalem artichoke as raw material enzyme hydrolysis
Inulin (also known as synanthrin nulin) produces oligofructose, and it is the oligomeric of 2-8 that the degree of polymerization, which can be obtained, with endo-type inulin enzyme hydrolysis in inulin
Fructose has good aqueous solubility, and in addition to containing fructooligosaccharide, it is poly- generally also to mix the fruit without terminal glucose saccharide residue on a small quantity
Sugar.When preparing oligofructose as raw material using white granulated sugar, sucrose is through transfructosylase or with the β-furans for turning fructosyl vigor
Fructose enzyme effect and generate oligofructose, mechanism is that the catalysis of the above-mentioned enzyme generated during the fermentation using microorganism is made
With, it carrying out intermolecular fructose transfer reaction and generates oligofructose, gained oligofructose polymerization scope is smaller (DP3-DP6),
Belong to sugarcane-fruit type.
In traditional dietotherapy, brown sugar or a kind of health products.Domestic mechanism sugar refinery is mostly with sulfurous method or carbonic acid
Method produces product white granulated sugar and a small amount of byproduct brown granulated sugar.As people's living standard improves, health care consciousness, consumption idea
Change, and the attention to Safety of Food Quality, diet-therapy health-preserving have become a popular topic, single white sand sugar product
Have been unable to meet the consumption demand of consumer.For present day schemes brown granulated sugar, brown sugar is not added in process of production appoints
What chemical assistant, only with physical method to extracting sugarcane juice, clarification, evaporation, crystallization, molding and packing processes.Folks of china
The brown sugar being commonly called as, traditional technique are to roll out sugarcane juice with stone stone roller or wood, and the bubble generated when using boiling skims solid
Impurity (silt brought in sugarcane chaff sugarcane silk or sugarcane), and stepped evaporation concentration is carried out, reach certain degree of supersaturation postcooling, and
Stirring stimulation graining, forms blocky or powdery product.The Sucrose products produced in this way are commonly referred to as " traditional brown sugar ",
It is a kind of ecosystem brown sugar due to being not added with any chemical assistant in production process.Containing there are many amino needed by human in sugarcane
Acid, such as lysine, glycine, leucine, glutamic acid, these amino acid be all synthesized human protein, support metabolism,
The indispensable basic substance of human life activity is participated in, is promoted health.It is remained without the ecosystem brown sugar of refining
Nutritional ingredient in sugarcane is more prone to be digested, therefore can quickly regain one's strength of body, enhance vigour, traditional brown sugar
It is referred to as " chocolate in east ", wherein not only containing the carbohydrate that can provide thermal energy, also contains growth in humans's development not
The various elements such as malic acid, riboflavin, carrotene, niacin and the trace element manganese that can lack, zinc, chromium.According to analysis, 1kg tradition
In brown sugar, contain calcareous 900mg, irony 40mg, than calcareous more 2 times contained by 1kg white granulated sugar, irony is 1 times more, phosphorus matter 10 again with
On, the content of manganese and zinc is all higher than white granulated sugar.
Although brown sugar has nutritive value more higher than white granulated sugar, mainly used when industrially prepared oligofructose at present
Jerusalem artichoke, witloof or white granulated sugar do not use brown sugar for raw material.
Invention content
The object of the present invention is to provide a kind of oligofructose prepared as raw material using brown sugar.It is prepared using brown sugar as raw material low
Fructooligosaccharides can promote the proliferation of Bifidobacterium in enteron aisle, inhibit enterobacteria, enterococcal proliferation, have and adjust intestinal flora
Effect.In addition, it inhibits enterobacteria and enterococcal effect to be substantially better than the oligofructose in white granulated sugar source.
The purpose of the present invention is what is be achieved through the following technical solutions：
A kind of oligofructose prepared as raw material using brown sugar, is prepared by following methods：
(1) immobilized fructosyltransferase is added in brown sugar solution and carries out conversion reaction, obtain syrup；
(2) it is separated by filtration syrup；
(3) after the syrup concentrated sterilization after being separated by filtration to obtain the final product.
Preferably, pH4.5-6.5 is converted, conversion temperature is 40-55 DEG C of progress 16h or more.
Preferably, it is separated by filtration and includes the following steps：After syrup is separated by filtration by 100-200 mesh screens, then it is used
Filter is separated by filtration.
Preferably, it is 60-78% the syrup after being separated by filtration to be concentrated into solid content.
Preferably, sterilizing carries out 10-20s at 121-135 DEG C.
Preferably, the oligofructose obtained is the oligofructose of content 48-55%.
A kind of oligofructose prepared as raw material using brown sugar further includes the steps that brown sugar solution is made in brown sugar.
Preferably, the mass percent of brown sugar solution is 40-60%.
It is further preferred that the mass percent of brown sugar solution is 45-55%.
Beneficial effects of the present invention：
The oligofructose of the present invention is converted by immobilized fructosyltransferase, be separated by filtration, concentrated using brown sugar as raw material
Oligofructose is made in brown sugar by sterilization steps.Bifidobacterium and Bacillus acidi lactici are beneficial microbes important in human body intestinal canal,
It evaluates in the Dietary frequency research that food adjusts intestinal flora function, Bifidobacterium is most common enteric microorganism index.With
Brown sugar is oligofructose prepared by raw material, can promote the proliferation of Bifidobacterium in enteron aisle, inhibits enterobacteria, enterococcal increasing
It grows, has the function of adjusting intestinal flora, and it inhibits enterobacteria and enterococcal effect to be substantially better than the low of white granulated sugar source
Fructooligosaccharides.
Specific implementation mode
Below in conjunction with specific embodiment, the invention will be further described.
Embodiment 1
A kind of oligofructose prepared as raw material using brown sugar, is prepared by following methods：
(1) brown sugar is made to the brown sugar solution of mass percent 45%；
(2) immobilized fructosyltransferase is added in brown sugar solution, is that 42 DEG C of conversions 26 are small in pH5.4, conversion temperature
When, obtain syrup；
(3) syrup is separated by filtration collection invertase by 100 mesh screens, then is separated by filtration with filter；
(4) it is 60% that the syrup after being separated by filtration is concentrated into solid content by vacuum concentration evaporator；
(5) syrup after concentrating sterilize 20s at 121 DEG C, obtains content as 48% oligofructose.
Embodiment 2
A kind of oligofructose prepared as raw material using brown sugar, is prepared by following methods：
(1) brown sugar is made to the brown sugar solution of mass percent 50%；
(2) immobilized fructosyltransferase is added in brown sugar solution, is that 50 DEG C of conversions 20 are small in pH6.5, conversion temperature
When, obtain syrup；
(3) syrup is separated by filtration collection invertase by 100 mesh screens, then is separated by filtration with filter；
(4) it is 69% that the syrup after being separated by filtration is concentrated into solid content by vacuum concentration evaporator；
(5) syrup after concentrating sterilize 15s at 125 DEG C, obtains content as 50% oligofructose.
Embodiment 3
A kind of oligofructose prepared as raw material using brown sugar, is prepared by following methods：
(1) brown sugar is made to the brown sugar solution of mass percent 55%；
(2) immobilized fructosyltransferase is added in brown sugar solution, is that 54 DEG C of conversions 17 are small in pH4.8, conversion temperature
When, obtain syrup；
(3) syrup is separated by filtration collection invertase by 100 mesh screens, then is separated by filtration with filter；
(4) it is 78% that the syrup after being separated by filtration is concentrated into solid content by vacuum concentration evaporator；
(5) syrup after concentrating sterilize 10s at 135 DEG C, obtains content as 55% oligofructose.
Embodiment 4
A kind of oligofructose prepared as raw material using brown sugar, is prepared by following methods：
(1) brown sugar is made to the brown sugar solution of mass percent 60%；
(2) immobilized fructosyltransferase is added in brown sugar solution, being 45 DEG C in pH6, conversion temperature converts 28 hours,
Obtain syrup；
(3) syrup is separated by filtration collection invertase by 200 mesh screens, then is separated by filtration with filter；
(4) it is 75% that the syrup after being separated by filtration is concentrated into solid content by vacuum concentration evaporator；
(5) syrup after concentrating sterilize 10s at 135 DEG C, obtains content as 53% oligofructose.
Test example
1. test material
Object：SD rats, age in days 70-80 days, female, 25 DEG C of raisings of room temperature.Feed is that mouse rat maintains mixed feed
(being free of non-nutritive additive).
Culture medium：BBL (Bifidobacterium), MRS (bifidobacterium lactis), EMB (enterobacteria), Sodium azide-crystal violet-aesculin
Agar (enterococcus), TSC (term capsular clostridium).
2. content of the test
Dosage selects：In terms of FOS sterlings, 30 times of dosage 10g/60kg BW are recommended to be used as test dose according to human body；
Test method：For brown sugar FOS and FOS group with FOS sterling content meters, setting dosage is 5g/kg BW, is equivalent to people
30 times of body recommended amounts.
Tested material gives mode：Oral gavage is primary daily, and gavage volume is 0.2mL/10g BW.Test group is according to volume
Amount prepares the tested material (FOS, brown sugar FOS) of respective concentration, the physiology salt of blank control group gavage respective volume amount with pure water
Water；To quadrat method it is gavage, test group distinguishes the oligofructose (red FOS) in 1 brown sugar source of gavage embodiment, white granulated sugar source
Oligofructose (FOS), blank group gavage physiological saline adjust tested material gavage amount according to changes of weight.
Test method：(25 DEG C, exhaust fan and Air conditioner air exchange) the adaptability culture in laboratory conditions of 30 female rats
After 7d, it is randomly divided into 3 groups, respectively blank group, brown sugar FOS groups, FOS groups.Sub-cage rearing, every rat ensure there is sufficient drinking-water
And supply of forage, continuous 28 days.Experiment starts and experiment periods are weighed daily, claims a feed surplus weekly, convenient for meter
Calculate gavage amount and feed intake.0 week on-test, 1 week, 2 weeks, 3 weeks, 4 weeks, take excrement sample to detect intestinal flora.
Observation index：(1) general indicator：Rat body weight；(2) intestinal flora：Detect Bifidobacterium, lactic acid bar in enteron aisle
Bacterium, enterobacteria, enterococcus, C.perfringens content.
3. test result
(1) rat body weight
(2) Bifidobacterium
* it indicates and blank group significant difference, P ＜ 0.05；* indicates, P ＜ 0.01 extremely notable with blank group difference；
A is indicated and 0 week significant difference of experiment, P ＜ 0.05.
At 0 week, each group intestine in rats Bifidobacterium content shows Bifidobacterium in each group intestine in rats without significant difference
Original state indifference.Blank group rat is during gavage, and intestinal bifidobacteria quantity is without significant change.Brown sugar FOS groups and FOS
Group rat during gavage, intestinal bifidobacteria quantity compare blank group showed increased, show brown sugar source oligofructose and
The oligofructose in white granulated sugar source can promote internal Bifidobacterium to be proliferated.Experiments have shown that, brown sugar FOS groups and FOS groups are big in group
The quantity of mouse Bifidobacterium than experiment 0 week when increased.Gavage stops after a week, and each group bifidobacteria significantly subtracts
It is few.
(3) Bacillus acidi lactici
* it indicates and blank group significant difference, P ＜ 0.05；* indicates, P ＜ 0.01 extremely notable with blank group difference；
A is indicated and 0 week significant difference of experiment, P ＜ 0.05；B is indicated and FOS group significant differences, P ＜ 0.05
At 0 week, the content of each group intestine in rats Bacillus acidi lactici shows lactic acid bar in each group intestine in rats without significant difference
Bacterium original state indifference.Brown sugar FOS groups and FOS groups rat are in the entire test period, and intestinal lactobacillus quantity is and the same period
Bacillus acidi lactici quantity is close in blank group, no difference of science of statistics.The result shows that with 30 multiple doses of human body recommended amounts 10g/60kg
Continuous gavage rat 4 weeks, the oligofructose in brown sugar source and the oligofructose in white granulated sugar source do not influence Bacillus acidi lactici in enteron aisle
Proliferation.
(4) enterobacteria
* it indicates and blank group significant difference, P ＜ 0.05；* indicates, P ＜ 0.01 extremely notable with blank group difference；
A is indicated and 0 week significant difference of experiment, P ＜ 0.05.
At 0 week, each group intestine in rats enterobacteria content shows in each group intestine in rats without significant difference at the beginning of enterobacteria
Beginning state indifference.During gavage, compared with blank group, only brown sugar FOS groups rat oral gavage after 3 weeks enterobacteria quantity occur it is bright
It is aobvious to reduce.In addition in the test period, enterobacteria quantity has than being reduced when on-test in brown sugar FOS group intestine in rats
Statistical significance P ＜ 0.05.The result shows that the oligofructose in brown sugar source to the adjustment effect of enterobacteria than white granulated sugar source
Oligofructose is apparent.
(5) enterococcus
* it indicates and blank group significant difference, P ＜ 0.05；* indicates, P ＜ 0.01 extremely notable with blank group difference；
A is indicated and 0 week significant difference of experiment, P ＜ 0.05.
At 0 week, each group intestine in rats enterococcus content shows in each group intestine in rats without significant difference at the beginning of enterococcus
Beginning state indifference.FOS groups and brown sugar FOS groups rat after gavage 1 week enterococcus quantity compared with on-test when increase, but with sky
Bai Zuwu significant differences.After gavage 2 weeks, 3 weeks, 4 weeks, compared with blank group, FOS groups and brown sugar FOS group rats are enterococcal
Quantity substantially reduces, and wherein brown sugar FOS groups rat has pole in gavage 2 weeks and 3 weeks rear intestinal enterococcus quantity compared with blank group
Significant difference.FOS groups enterococcus quantity after gavage 3 weeks has pole significant difference compared with blank group.The result shows that brown sugar comes
The oligofructose in source is better than enterococcal inhibiting effect the oligofructose in white granulated sugar source.
(6) C.perfringens
* it indicates and blank group significant difference, P ＜ 0.05；* indicates, P ＜ 0.01 extremely notable with blank group difference；
A is indicated and 0 week significant difference of experiment, P ＜ 0.05.
At 0 week, each group intestine in rats C.perfringens content shows to produce in each group intestine in rats without significant difference
Gas capsular clostridium original state indifference.When gavage 1 week, brown sugar FOS groups and FOS group intestine in rats C.perfringens quantity are equal
It is significantly fewer than control group.Then during subsequent gavage, brown sugar FOS groups and FOS groups rat C.perfringens quantity and blank
Group indifference.The result shows that with the 30 multiple dose continuous gavage rat 4 weeks of human body recommended amounts 10g/60kg, brown sugar source it is low
The oligofructose in Fructooligosaccharides and white granulated sugar source does not influence the proliferation of C.perfringens in enteron aisle.
4. conclusion
The oligofructose and white sand in the continuous 4 weeks 30 multiple dose gavage rat brown sugar sources with human body recommended amounts 10g/60kg
The oligofructose in sugared source, on SD rat body weights without influence.
Coliform shows (to test 0 week) in on-test, Bifidobacterium, lactic acid in each group intestine in rats
Bacillus, enterobacteria, enterococcus and C.perfringens content are without significant difference.During experiment, the oligofructose in brown sugar source
The quantity of intestine in rats Bifidobacterium can be obviously increased with the oligofructose in white granulated sugar source, but Bacillus acidi lactici is increased without apparent
The effect of growing.Compared with the oligofructose in white granulated sugar source, the oligofructose in brown sugar source makees enterobacteria and enterococcal adjusting
With becoming apparent from.In addition, each group C.perfringens content is without significant change.
To sum up, the oligofructose in brown sugar source to Bacteria from Gl Tract of Rats adjustment effect in the oligofructose phase in white granulated sugar source
Seemingly, it is proliferated Bifidobacterium, inhibits enterobacteria and enterococcus, but the oligofructose in brown sugar source is to enterobacteria and enterococcal suppression
It makes of the oligofructose better than white granulated sugar source.
Claims (9)
1. a kind of oligofructose prepared as raw material using brown sugar, which is characterized in that prepared by following methods：
(1) immobilized fructosyltransferase is added in brown sugar solution and carries out conversion reaction, obtain syrup；
(2) it is separated by filtration syrup；
(3) after the syrup concentrated sterilization after being separated by filtration to obtain the final product.
2. a kind of oligofructose prepared as raw material using brown sugar according to claim 1, which is characterized in that conversion reaction exists
PH4.5-6.5, conversion temperature are 40-55 DEG C of progress 16h or more.
3. a kind of oligofructose prepared as raw material using brown sugar according to claim 1, which is characterized in that be separated by filtration packet
Include following steps：After syrup is separated by filtration by 100-200 mesh screens, then it is separated by filtration with filter.
4. a kind of oligofructose prepared as raw material using brown sugar according to claim 1, which is characterized in that will be separated by filtration
It is 60-78% that syrup afterwards, which is concentrated into solid content,.
5. a kind of oligofructose prepared as raw material using brown sugar according to claim 1, which is characterized in that sterilizing is in 121-
135 DEG C of progress 10-20s.
6. a kind of oligofructose prepared as raw material using brown sugar according to claim 1, which is characterized in that is obtained is oligomeric
Fructose is the oligofructose of content 48%-55%.
7. a kind of oligofructose prepared as raw material using brown sugar according to claim 1, which is characterized in that further including will be red
The step of brown sugar solution is made in sugar.
8. a kind of oligofructose prepared as raw material using brown sugar according to claim 7, which is characterized in that the brown sugar is molten
The mass percent of liquid is 40-60%.
9. a kind of oligofructose prepared as raw material using brown sugar according to claim 8, which is characterized in that the brown sugar is molten
The mass percent of liquid is 45-55%.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111109421A (en) * | 2019-12-31 | 2020-05-08 | 量子高科(中国)生物股份有限公司 | Extractum containing brown sugar fructo-oligosaccharide and preparation method thereof |
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