CN108324591A

CN108324591A - A kind of hair composition containing marine bioactivity object

Info

Publication number: CN108324591A
Application number: CN201810150767.0A
Authority: CN
Inventors: 陈绍昌; 陈华; 陈忻; 陈得科; 孙恢礼; 何忠东; 陈家良; 庞秀枰
Original assignee: Foshan An'an Beauty & Health Products Co ltd; South China Sea Institute of Oceanology of CAS; Foshan University
Current assignee: Foshan An'an Beauty & Health Products Co ltd; South China Sea Institute of Oceanology of CAS; Foshan University
Priority date: 2018-02-13
Filing date: 2018-02-13
Publication date: 2018-07-27
Anticipated expiration: 2038-02-13
Also published as: CN108324591B

Abstract

The invention discloses a kind of hair compositions containing marine bioactivity object, specifically contain D aminoglucose hydrochlorides, seashells peptide, fish protein peptide, algal polysaccharides and piroctone olamine salt, and can further be aided with olive oil and panthenol.Hair composition of the present invention can be used as the hair washing product with special anti-dandruff function, such as anti-dandruff shampoo, be repaired using back skin cuticula structure and normal barrier function.The hair composition has the multi-efficiencies such as lasting moisturizing, Bergamot Mint Extract, antibacterial, anti-inflammatory, anti-dandruff, antipruritic, moreover it is possible to significantly improve hair quality, reduce bifurcated, alleviates alopecia, anti-dandruff and hair care can be made to be carried out at the same time.

Description

A kind of hair composition containing marine bioactivity object

Technical field

The invention belongs to cosmetic fields, and in particular to a kind of hair composition, more particularly to one kind is with D- aminoglucoses Sugared hydrochloride, seashells peptide, fish protein peptide, algal polysaccharides, piroctone olamine salt be active constituent, have it is antibacterial, disappear Composition that is scorching, anti-dandruff, antipruritic, repairing scalp stratum corneum barrier, improve hair quality function.

Background technology

According to incompletely statistics, crowd more than global puberty later half is perplexed by dandruff.Typical dandruff is finger Laminar, the pruritic dandruff of skin adhesive surface, the white occurred when being scalp abnormality or the grey scales of skin that peel off.This scales of skin that peel off Grain is larger, combs one's hair or scratches and easily falls to shoulder when grabbing, and not only influences beautiful, can also give the work, life and social band of people To perplex.

Dandruff is formed to be influenced by many factors such as chlosma field planting, sebum secretion and private medical services, defective Stratum corneum barrier be the key factor for causing dandruff, fragile Damages of Epidermal Permeability Barrier using shampoo because frequently being more easy to be damaged. Surfactant in shampoo can reduce water-soluble natural moisturizing factor and cuticula enzymatic activity, the surface-active of strong and stimulating Agent can then aggravate the fragile Damages of Epidermal Permeability Barrier that damage lipid level has declined, wash rear scalp often will appear tight feeling, drying, The inflammatory reactions such as stimulation, itch.Serious or chronic cuticle loss can then make moisture rapidly scatter and disappear, epidermis paraplasm, cell Hyperkeratinization, cuticula structured lipid missing and disorderly arranged, skin natural cover for defense function gets muddled, and then generates a large amount of heads Bits.

Currently, the commercially available most therapy approach of anti-dandruff product is single, the cutin of the excessive scalp of dandruff can not be directly repaired Layer damage.And how much amount reaches antibacterial, sterilization purpose using anti-dandruff agents such as salicylic acid, zinc pyrithione, Climbazoles, easily damages Hinder hair hair scale piece.It hair is used for a long time can become to dry up, bifurcated, it is serious it is also possible that allergic reaction, and then lead to head It considers periodicity to be worth doing or duration occurs, or even scalp hair follicles is caused to damage, cause permanent alopecia.Therefore, antibacterial, anti-inflammatory, it is anti-dandruff, Antipruritic aspect, existing product are also insufficient for demand.Field is had one's hair wash at present in terms of improving scalp stratum corneum barrier function, Lack effective product and research.

Marine bioactivity object is the functional product for preparing marine organisms through green technologies such as enzymolysis, is contained Abundant active constituent improves there are some researches prove it is conducive to the moisturizing of skin, anti-inflammatory and barrier function.Such as patent ZL201510777169.2 discloses D-Glucosamine Hydrochloride and can be used for facial mask, has not to bacterium, saccharomycete, fungi etc. With the inhibiting effect of degree；Seashells peptide is used for cosmetics by patent ZL201110079404.0, is had and is promoted collagen The effects that generating with wound healing, raising skin natural cover for defense function；Patent ZL201410294532.0 uses seashells peptide In hair products, epilation can be reduced；Algal polysaccharides are used for cosmetics by patent ZL201110288716.2, can be in skin table Face forms thin film, has effects that good moisturizing and repairs.

Invention content

It is an object of the present invention to provide a kind of Haircare compositions.

It is another object of the present invention to provide one kind can effectively improve scalp cuticula structure and normal barrier function Composition.

It is another object of the present invention to provide a kind of energy persistently moisturizing, the compositions of Bergamot Mint Extract effect.

There is antibacterial, anti-inflammatory, anti-dandruff, itching-relieving efficacies composition it is another object of the present invention to provide a kind of.

It is another object of the present invention to provide a kind of compositions that can improve hair quality, reduce bifurcated, alleviate alopecia.

Invention is achieved through the following technical solutions above-mentioned purpose：

On the one hand, the present invention provides a kind of compositions, and containing D-Glucosamine Hydrochloride, the composition is for protecting Hair care silk or the product for preparing protection hair.

As preferred embodiment, the composition is used to prepare the product of protection hair, D-Glucosamine Hydrochloride Mass percentage content in the product is 0.01%-0.1%.Embodiment more preferably, D- glucosamine salts The mass percentage content of hydrochlorate in the product is 0.01%-0.05%.

Known D-Glucosamine Hydrochloride has good efficacy to treatment rheumatism joint inflammation and gastric ulcer, has and protects Wet, antibacterial, antiinflammation, can be used for face skin nursing.But very it is related to less at present about its research for taking care of hair effect and product. Inventor has found that D-Glucosamine Hydrochloride has film forming and protection hair effect, can reduce surface work for the first time by research Property agent and hair protein binding, help establishes antibacterial barrier, chlosma metabolite and other microbial source toxin made to be not easy Invasion.

D-Glucosamine Hydrochloride can be obtained by any of method.For example, passing through commercially available acquisition.Or Person is extracted or is prepared by existing method, for example, being obtained by natural chitin extraction.

As a kind of optional scheme, the preparation methods of D- amino glucose hydrochlorides be by chitosan through hydrochloric acid hydrolysis and At.For example, weighing a certain amount of chitosan, the dilute hydrochloric acid that 3-5 times of volumetric concentration is 10%-15%, 80 DEG C -90 of temperature is added DEG C, 3h-4h is reacted, is filtered, filtered solution addition activated carbon (quality is the 1%-2% of liquor capacity), 65 DEG C -70 DEG C of temperature, instead 2h-3h to be answered, is filtered, the absolute ethyl alcohol of certain volume is added to ethyl alcohol final concentration 65-85% in filtered solution, 0-8 DEG C of standing 8h- 12h, filtering obtain D-Glucosamine Hydrochloride crystallization.

For example, more specifically, chitosan is hydrolyzed through hydrochloric acid.For example, weighing a certain amount of chitosan, it is added 5 times The dilute hydrochloric acid that volumetric concentration is 15%, 90 DEG C of temperature react 4h, filtering, and activated carbon is added in filtered solution, and (quality is liquor capacity 2%), temperature 70 C reacts 2h, filtering, and the absolute ethyl alcohol of certain volume is added to ethyl alcohol final concentration 75% in filtered solution, and 4 DEG C quiet 12h is set, filtering obtains D-Glucosamine Hydrochloride crystallization.

Further, another aspect of the present invention provides a kind of composition containing marine bioactivity object, the composition Contain D-Glucosamine Hydrochloride, seashells peptide, fish protein peptide, algal polysaccharides and piroctone olamine salt.

Embodiment as one preferred, composition of the invention contain the component of following parts by weight：D- amino Portugal 1-10 parts of grape sugar hydrochloride, 1-10 parts of seashells peptide, 1-10 parts of fish protein peptide, 1-10 parts of algal polysaccharides, piroctone ethyl alcohol 10-100 parts of amine salt；Embodiment more preferably contains the component of following parts by weight in composition of the invention：1-5 Part D-Glucosamine Hydrochloride, 1-5 parts of seashells peptides, 1-5 parts of fish protein peptides, 1-5 parts of algal polysaccharides, 10-75 parts of pyrrole sieve Gram ketone ethanolamine salt.

The preparation method and effect of wherein D-Glucosamine Hydrochloride are as described above.

In above-mentioned active constituent, the seashells peptide is ocean shellfish meat by the product obtained by protease hydrolyzed.This Preceding inventor uses it for skin care, hair care research obtains good effect.However due to generating scalp and human body when dandruff The skin at other positions has no substance of the report for normal skin care at present in difference present on physiological status and structure It can be used for the nursing of scalp under pathological state.Molecular weight ranges are for the first time that 1KDa-3KDa seashells peptides are used for head by inventor It considers to be worth doing under pathological state in the nursing of scalp, it is found that after it is absorbed by scalp, scalp surface moisture loss can be reduced, hair follicle stimulating, Improve microcirculation, improves SOD, CAT, GPx activity in skin histology, while anti-dandruff agent can be reduced to scalp irritation, reduce inflammation Damaged skin structure and normal barrier function are repaired in reaction, improve resistance of the scalp to bacterium, fungi, and then reduce dandruff It generates.

These seashells can include but is not limited to one kind in paphia undulata, pearl shell, scallop, mussel and oyster Or several combination.And used protease can be the enzyme that can digest albumen, including but not limited to Papain The combination of one or both of enzyme, alkali protease.

As a kind of optional embodiment, the preparation method of seashells peptide is separation after shellfish meat adds protease hydrolytic Go out the peptide that molecular weight is less than 3KDa；More preferably greater than 1KDa and less than 3KDa peptide；It is specific as follows：Internal organ are removed in seashells decladding Afterwards plus 3-5 times of volume pure water homogenate, addition protease (2000U/g shellfish meat -3000U/g shellfish meats) are 6.5-7.5, temperature in pH 40 DEG C -50 DEG C of degree, digests 4h-6h, and boiling water bath enzyme deactivation 8-12min, 8000r/m-10000r/m centrifuge 10min-15min, supernatant Liquid passes through molecular cut off 1KDa ultrafiltration membrane ultrafiltration, trapped fluid spray again by molecular cut off 3KDa ultrafiltration membrane ultrafiltration, filtered solution Mist is dried.

For example, more specifically, seashells decladding is gone after internal organ to add 4 times of volume pure water homogenate, addition protease 3000U/g shellfish meats are 7 in pH, temperature 50 C, digest 4h, and boiling water bath enzyme deactivation 10min, 8000r/m centrifuge 15min, and supernatant is logical Molecular cut off 3KDa ultrafiltration membrane ultrafiltration is crossed, for filtered solution again by molecular cut off 1KDa ultrafiltration membrane ultrafiltration, trapped fluid spraying is dry It is dry to form.

In above-mentioned active constituent, the fish protein peptide is ocean fish by the product obtained by protease hydrolyzed, can be delayed And skin tightness, surfactant is reduced to scalp irritation and albumen and lipid membrane damage, maintains cuticula integrality and water Balance-dividing.These marine fishes can include but is not limited to the combination of one or both of tuna and salmon.And it is adopted Protease can be the enzyme that can digest albumen, including but not limited to alkali protease.

As a kind of embodiment, the preparation method of fish protein peptide is after the flesh of fish adds protease hydrolytic, to isolate molecular weight Peptide less than 10KDa；It is specific as follows：It takes ocean fish to add 3-5 times of volume pure water homogenate, alkali protease, enzyme concentration is added It is 6.5-7.5 in pH, temperature is 40 DEG C -55 DEG C, digests 4h-6h, boiling water bath enzyme deactivation for the 3000U/g flesh of fish -4000U/g flesh of fish 8-12min, 8000-10000r/m centrifuge 10min-15min, and supernatant is 10KDa ultrafiltration membrane ultrafiltration, filter by molecular cut off Liquid is crossed to be spray-dried.

For example, more specifically, ocean fish is taken to add the homogenate of 3 times of volume pure water, alkali protease is added, enzyme concentration is The 4000U/g flesh of fish is 7 in pH, and temperature is 50 DEG C, digests 5h, and boiling water bath enzyme deactivation 10min, 8000r/m centrifuge 15min, supernatant It is 10KDa ultrafiltration membrane ultrafiltration by molecular cut off, filtered solution is spray-dried.

Algal polysaccharides are common raw materials in cosmetics, field of food and medicine etc..Algal polysaccharides are that seaweed passes through cellulose Product obtained by enzyme and pectinase enzymatic hydrolysis.These seaweed can include but is not limited to seaweed, sargassum fusifome, kelp, spirulina, huge The combination of one or more of algae, yellow tang, bladder-wrack etc..In the compositions of the present invention, algal polysaccharides can reduce skin table Face moisture loss, Bergamot Mint Extract (because reducing P.ovale source of nutrition, and then reduce chlosma and grow), dimension Cuticula moisture and intrinsic activity are held, nutriment osmotic absorption is promoted, maintains scalp complete and health.

The source of algal polysaccharides can be bought by commercial sources and be obtained, and can also be prepared by enzymolysis.

For example, as a kind of optional embodiment, the preparation method of algal polysaccharides is that seaweed adds cellulase and pectase The enzymolysis product that molecular weight is more than 5KDa is isolated in hydrolysis；It is specific as follows：Seaweed is cleaned, blends, and it is pure that 4-6 times of volume is added Water is 6.5-7.5 in pH, and temperature is 60 DEG C -75 DEG C, and cellulase (3000U/g seaweed -4000U/g seaweed) and pectin is added Enzyme (3000U/g seaweed -4000U/g seaweed) digests 3h-5h, boiling water bath enzyme deactivation 8-12min, 8000r/m-10000r/m centrifugation 10min-15min.Supernatant is 5KDa ultrafiltration membrane ultrafiltration by molecular cut off, and 50 DEG C -80 DEG C of trapped fluid is concentrated into original volume 1/4,0-8 DEG C of absolute ethyl alcohol of precooling is added after cooling to ethyl alcohol final concentration 65-85%, filters, precipitation freeze-drying is algal polysaccharides.

For example, more specifically, seaweed is cleaned, blends, 5 times of volume pure water of addition are 7 in pH, and temperature is 70 DEG C, is added Cellulase (4000U/g seaweed) and pectase (3000U/g seaweed) digest 5h, boiling water bath enzyme deactivation 10min, 8000r/m centrifugation 15min.Supernatant is 5KDa ultrafiltration membrane ultrafiltration by molecular cut off, and 80 DEG C of trapped fluid is concentrated into original volume 1/4, adds after cooling Enter 4 DEG C of absolute ethyl alcohols of precooling to ethyl alcohol final concentration 75%, filter, precipitation freeze-drying is algal polysaccharides.

Piroctone olamine salt is a kind of antidandruff agent, can alleviate scalp irritation, reduces dandruff and peels off, and is a kind of The antibacterial of wide spectrum, bacteriostatic agent.Piroctone olamine salt is safe and nontoxic, non-stimulated, can effectively kill the yeast for generating dandruff Bacterium inhibits Gram-positive, feminine gender and various fungies and fungus growth, and solubility property and compound property are excellent, with cosmetics The effects that raw material mixing will not be precipitated or be layered, have both thickening, anti-corrosion and deodorization.

As preferred embodiment, repairs scalp stratum corneum barrier for enhancing, improves hair quality effect, form the present invention's Olive oil, 10-100 parts by weight panthenols in composition also containing 10-100 parts by weight.It is preferred that 10-50 wt parts of olive oil, 10- The panthenol of 50 parts by weight.

The olive oil can penetrate into cuticula, keep out surfactant micellar extracting skin lipid, additional clean process The Endogenous fatty acid that middle skin surface is lost, improves structured lipid level, and repairing skin tissue's structure helps improve skin screen Hinder function.The panthenol is easily absorbed by root of hair, enhances hair follicle vigor, and lasting moisturizing promotes hair tonic, reduces dandruff, It easily is adsorbed in hair, prevents hair bifurcated, impaired, increase hair density, improvement hair quality from keeping hair submissive and rich in gloss.

In mentioned component, the D-Glucosamine Hydrochloride, seashells peptide, fish protein peptide, algal polysaccharides, pyrrole sieve Gram ketone ethanolamine salt, olive oil, panthenol can be prepared according to existing method oneself, also can be from purchased in market.

In a kind of preferred scheme of the present invention, the composition is used to prepare or is used as hair products；Preferably, it uses In preparation or it is used as shampoo；It is particularly suitable for use as having the shampoo of special anti-dandruff function to use.In addition to this, this hair Bright composition can significantly improve the skin condition performance of product；Scalp fat secretion is balanced, including hair gloss can be made apparent It is improved, makes hair is smooth to be easier to arrange, hair entanglement during rinsing can be effectively removed, effectively prevent hair short-tempered, etc. Deng.

When the composition is used to prepare or is used as shampoo, weight hundred of each component in shampoo in the composition Score is D-Glucosamine Hydrochloride 0.01%-0.1%, seashells peptide 0.01%-0.1%, fish protein peptide 0.01%- 0.1%, algal polysaccharides 0.01%-0.1%, piroctone olamine salt 0.1%-1.0%.

As a preferred embodiment, weight percent of each component in shampoo is in the composition：D- ammonia Base glucosamine salt hydrochlorate 0.01%-0.05%, seashells peptide 0.01%-0.05%, fish protein peptide 0.01%-0.05%, sea Polysaccharides 0.01%-0.05%, piroctone olamine salt 0.1%-0.75%.

Alternatively, alternatively preferred embodiment, the composition is used to prepare or when as shampoo, the group It is D-Glucosamine Hydrochloride 0.01%-0.1%, seashells peptide to close weight percent of each component in shampoo in object 0.01%-0.1%, fish protein peptide 0.01%-0.1%, algal polysaccharides 0.01%-0.1%, piroctone olamine salt 0.1%- 1.0%, olive oil 0.1%-1.0%, panthenol 0.1%-1.0%.

When either alternatively preferred embodiment the composition is used to prepare or is used as shampoo, the group It is D-Glucosamine Hydrochloride 0.01%-0.05%, seashells to close weight percent of each component in shampoo in object Peptide 0.01%-0.05%, fish protein peptide 0.01%-0.05%, algal polysaccharides 0.01%-0.05%, piroctone olamine salt 0.1%-0.75%, olive oil 0.1%-0.5%, panthenol 0.1%-0.5%.

Combinations of the above object is used to prepare or is used as shampoo, can also include the matrix components such as surface of shampoo Activating agent, surfactant can be that composition brings cleaning action and frothing capacity.The surfactant is without special It limits, in the prior art the common surfactant of shampoo.Because composition provided by the invention as it is functional at Point, the stimulation of surfactant and anti-dandruff agent to scalp can be effectively reduced, in addition there are a variety of specific functions, such as moisturizing to protect Wet, Bergamot Mint Extract etc..Therefore, then broad for the selection of kinds of surfactants, can be selected from anion table At least one of face activating agent, zwitterionic surfactant and nonionic surfactant.

The example of the anion surfactant group includes but not limited to sulfate, sulfonate, sarcosinate and flesh ammonia Acid derivative.Preferred anion surfactant is alkyl and alkyl ether sulfate.Alkyl sulfate such as lauryl sulfate Ammonium, cocoyl ammonium sulfate, lauryl potassium sulfate, cocoyl potassium sulfate, NaLS, sodium cocosulfate, lauryl sulphur Sour monoethanolamine, cocoyl diethanolamine, lauryl sulfate diethanolamine, triethanolamine lauryl sulfate, lauryl sulphur Triethylenetetraminehexaacetic acid amine and its mixture；Alkyl ether sulfate such as polyoxyethylene lauryl ether ammonium sulfate, polyoxyethylene lauryl ether sulfuric acid Potassium, sodium laureth sulfate, polyoxyethylene lauryl ether diethanolamine, polyoxyethylene lauryl ether sulfuric acid two Ethanol amine, polyoxyethylene lauryl ether sulfuric acid triethanolamine, polyoxyethylene lauryl ether sulfuric acid triethylamine and its mixture.Sulfonic acid The anion surfactant of salt such as tridecyl benzene sulfonate, neopelex, lauryl sulfosuccinates Deng.Sarcosine and sarcosine derivative are as included sodium lauryl sarcosinate, cocoyl sarcosine, cocoyl sarcosine and its mixing Object, wherein it is preferred that sodium lauryl sarcosinate.Other applicable anion surfactants be derived from those of amino acid, including But be not limited to N- acyl groups-Pidolidone, N- acyl groups sarcosine and its salt etc..In addition, suitable anion surfactant may be used also Including taurine derivatives, such as 2-aminoethanesulfonic acid, N- acyl-N-methyl taurines.Other suitable anionic surfaces Activating agent is the product after aliphatic acid eterified with isethionic, after being neutralized with sodium hydroxide, and wherein aliphatic acid is, for example, and is derived from The fatty acid amide or sylvite of the aliphatic acid or methyl taurate of cocounut oil or palm oil.Other applicable anionic surfaces Activating agent is succinate, such as N- disodium octadecyl sulfosuccinates, the diamyl ester of sodium sulfosuccinate, sodium sulfosuccinate Dibutyl ester etc..

Wherein, the nonionic surfactant is it is known in the art that for example former with hydrophobic group and reactive hydrogen The compound of son, such as the polyoxyethylene ether of alkyl phenol, fatty alcohol polyoxyethylene ether, spans, Tweens, hydrogenated castor oils Deng.

Wherein amphoteric surfactant be it is known in the art that including but not limited to betaines zwitterionic compound, Including alkyl betaine, sulfobetaines, amidoalkyl betaine, amidosulfo betaines, hydroxyl sulfo betaine, coconut palm Oleoyl amidopropyl betaine, Cocamidopropyl hydroxyl sulfo betaine, cocoyl dimethyl carboxymethyl betaine, coconut palm Oleoyl dimethyl sulfopropyl betaine, stearyl dimethyl sulfopropyl glycine betaine, lauryl amide groups pair-(2- hydroxyl second Base) propylsultaine glycine betaine；Or imidazoline type amphoteric surfactant, such as 2- cocoyl-N- carboxymethyl group-N- hydroxyl second Base imidazolinium betaine, 2- lauryl-N- carboxymethyl group-N- hydroxyethyl imidazolines glycine betaines etc..

It can also include cationic polymer when the composition is used to prepare or is used as shampoo, it is possible to provide conditioning Effect, especially when hair soaks in use, by helping that conditioning effect will be played on conditioning agent deposit to hair and scalp. Cationic polymer in the composition of the present invention may include following substance：Polymeric quaternary ammonium salts, cationic polysaccharide, vinyl list Precursor copolymer, vinylpyrrolidone copolymer and cation-modified protein.

In addition to the above components, composition of the invention is used to prepare or as shampoo also optionally including with the following group It is one or more in point：Suspending agent, natural on-off cycles of hair growth conditioning agent, antistatic agent, foam booster, viscosity modifier and thickening Agent, pH adjusting agent, opacifier or pearling agent, dyestuff, organic solvent or diluent etc..These components and its content can be according to products Actual needs suitably selected, and be added to the present invention composition in.

The all components used in the present invention should be that skin is acceptable and do not influence the performance of the original component of the present invention, When i.e. when the skin with people is in contact or with other components compatibility, unsuitable toxicity, incompatibility, shakiness will not be caused Qualitative and allergic reaction etc..

In addition to above-mentioned component, composition of the invention can also contain suitable water." suitable water " in the present invention Mean that water adjusts according to actual needs.Without containing other components, " suitable water " can mean that " surplus is Water ".

The composition of the present invention can effectively reduce the stimulation of surfactant and anti-dandruff agent to scalp, keep skin wet, Bergamot Mint Extract makes scalp cuticula structure and normal barrier function effectively repair, and also has antibacterial, anti-inflammatory and other effects.Make With rear, itching of the scalp and clast, hair be greasy, bifurcated and phenomena such as falling off are improved, hair becomes submissive easy comb, rich in light Pool.

The solution of the present invention is based on inventor to dandruff patient's scalp corneocyte biophysics, bioid And Change of Ultrastructure influence dandruff and generate key factors and the corresponding therapies such as the cause of disease of development and susceptible mechanism, And the understanding to each functional activity substance effect, in conjunction with modern cosmetics and pharmacological Research Achievement, with D- aminoglucoses Based on sugared hydrochloride, seashells peptide, fish protein peptide, algal polysaccharides, piroctone olamine salt, and from numerous active materials In preferably go out olive oil and panthenol with synergy, carry out scientific composition and collaboration compound, preferably provided significantly anti-dandruff The shampoo formula and technique of effect.

The present invention has following advantageous effect outstanding：

(1) it is directed to dandruff patient's scalp cuticula special physiological to construct, anti-dandruff shampoo of the present invention preferably goes out pure natural Seashells peptide, fish protein peptide and safe and nontoxic, non-stimulated anti-dandruff agent piroctone olamine salt, utilize D- amino The film forming characteristics of glucosamine salt hydrochlorate and the humectant properties of algal polysaccharides are reducing surfactant and anti-dandruff agent to hair egg In vain, while scalp cuticula causes damage, promote nutriment Transdermal absorption, Bergamot Mint Extract, by establishing antibacterial screen Barrier repairs damaged Stratum Corneum structure and function, plays excellent anti-dandruff, itching-relieving efficacies.

(2) composition of the invention from effective surfactant and anti-dandruff agent of reducing to the stimulation of scalp, replenishing water and preserving moisture, flat Weighing apparatus fat secretion repairs scalp cuticula structure and the various aspects such as normal barrier function, antibacterial, anti-inflammatory and plays a role, it is antipruritic, While anti-dandruff, also has effects that good improvement hair quality.

(3) present invention utilizes D-Glucosamine Hydrochloride, seashells peptide, fish protein peptide, algal polysaccharides, pyrrole Rocks Stabilisation synergistic function between ketone ethanolamine salt and olive oil, panthenol makes its Bergamot Mint Extract, repairs cuticula screen Barrier improves the raising of hair quality obvious effect.

(4) composition of the invention is made of natural component, mildly, is not stimulated, is overcome existing anti-dandruff shampoo and easily draw Dandruff and hair damage and caused by itching of the scalp, dandruff cyclic recurrence, alopecia the problems such as.

Description of the drawings

Fig. 1 measures for sterile distilled water inhibition zone；

Fig. 2 measures for piroctone olamine salt aqueous solution inhibition zone；

Fig. 3 measures for sample A1 inhibition zones；

Fig. 4 measures for sample A2 inhibition zones；

Fig. 5 measures for sample A3 inhibition zones；

Fig. 6 measures for sample B1 inhibition zones；

Fig. 7 measures for sample B2 inhibition zones；

Fig. 8 measures for sample B3 inhibition zones.

Specific implementation mode

The invention will be further described below, but embodiments of the present invention are not limited to embodiment introduction below, Equivalent variation made by all methods according to the present invention or the flexible scope for being regarded as the present invention and protecting.Original used below Material, is commercially available in addition to special indicate.

The raw material information that embodiment uses：

Chinese:D-Glucosamine Hydrochloride

English name:D-Glucosamine Hydrochloride

CAS:66-84-2；

Chinese:Piroctone olamine salt

English name:Piroctone Olamine

CAS:68890-66-4。

In one embodiment of the present invention, with the D-Glucosamine Hydrochloride of 0.01%-0.1%, 0.01%- 0.1% seashells peptide, the fish protein peptide of 0.01%-0.1%, the algal polysaccharides of 0.01%-0.1%, 0.1%-1.0% Piroctone olamine salt is after the anti-dandruff shampoo of active constituent cleans hair, and without D-Glucosamine Hydrochloride, sea Foreign shellfish peptide, fish protein peptide, algal polysaccharides, piroctone olamine salt reference substance compare, as a result show using ammonia containing D- Base glucosamine salt hydrochlorate, seashells peptide, fish protein peptide, algal polysaccharides, piroctone olamine salt anti-dandruff shampoo after, head Bits scoring improves 2.83-3.13 times (about 17.4-19.0), itch scoring improves 2.68-2.97 times of (about 15.3-17.5), horse traction Color bacterium clump count improves 5.58-7.77 times (25.2/cm²- 26.5/cm²)。

In a preferred embodiment of the invention, with the D-Glucosamine Hydrochloride of 0.01%-0.1%, 0.01%- 0.1% seashells peptide, the fish protein peptide of 0.01%-0.1%, the algal polysaccharides of 0.01%-0.1%, 0.1%-1.0% Piroctone olamine salt, 0.1%-1.0% olive oil, 0.1%-1.0% panthenols are main component, can be equipped with other hair washings Reveal conventional cosmetic matrix, anti-dandruff shampoo is made as laboratory sample；Control sample is without containing D- glucosamine hydrochloric acids The shampoo substrate of salt, seashells peptide, fish protein peptide, algal polysaccharides, piroctone olamine salt, olive oil, panthenol.As a result It has been shown that, compared with control sample, the scalp handled by the test specimen containing these ingredients, dandruff scoring improves 3.73-3.96 (about 23.4-24.9), itch scoring improve 3.59-3.83 times (about 21.5-20.2), chlosma clump count improvement 5.03- again 7.88 times (25.0/cm²- 28.2/cm²)。

It for the scheme that the present invention is furture elucidated, and is easy to use, following embodiment is to prepare anti-dandruff shampoo Example, contains cosmetics conventional substrate.But what those skilled in the art should approve, which is component contained by these examples, to make For the limitation of the present invention program, and it is only a kind of enforceable alternative mode.Using main work of the present invention Property ingredient (D-Glucosamine Hydrochloride, seashells peptide, fish protein peptide, algal polysaccharides and piroctone olamine salt, olive Oil, panthenol), it by common process and arbitrary a variety of shampoo substrate component homogeneous or mixes, you can be made of the invention anti-dandruff Shampoo.For example, by embodiment 4-6 in addition to D-Glucosamine Hydrochloride, seashells peptide, fish protein peptide, algal polysaccharides, Other substances except piroctone olamine salt, olive oil and panthenol carry out conventional replacement, and other types or mutually similar are made The hair washing product of type, also will be with similar advantageous effect.

Embodiment 1

It weighs and meets the chitosan that China's correlation marine product uses standard, the dilute hydrochloric acid that 5 times of volumetric concentrations are 15% is added, 90 DEG C of temperature reacts 4h, filtering, and activated carbon (quality is the 2% of liquor capacity) is added in filtered solution, and temperature 70 C reacts 2h, mistake The absolute ethyl alcohol of certain volume is added to ethyl alcohol final concentration 75% in filter, filtered solution, and 4 DEG C of standing 12h are filtered and obtained D- aminoglucoses Sugared crystal of hydrochloride.

It takes and meets the edible paphia undulata soft body that China's correlation marine product uses standard, decladding adds after removing internal organ 4 times of volume pure water homogenate, are added papain (3000U/g shellfish meats), are 7 in pH, temperature 50 C digests 4h, boiling water bath Enzyme deactivation 10min, 8000r/m centrifuge 15min, and supernatant is by molecular cut off 3KDa ultrafiltration membrane ultrafiltration, and filtered solution is again by cutting Stay molecular weight 1KDa ultrafiltration membrane ultrafiltration, trapped fluid spray drying that paphia undulata peptide is made.

It takes and meets the edible tuna that China's correlation marine product uses standard, add 3 times of volume pure water homogenate, add Enter alkali protease (the 4000U/g flesh of fish), pH be 7, temperature be 50 DEG C, digest 5h, boiling water bath enzyme deactivation 10min, 8000r/m from Heart 15min, supernatant are 10KDa ultrafiltration membrane ultrafiltration by molecular cut off, and tuna protein peptides are made in filtered solution spray drying.

It weighs respectively and meets edible seaweed and kelp that China's correlation marine product uses standard, clean, blend, be added 5 times of volume pure water are 7 in pH, and temperature is 70 DEG C, and cellulase (4000U/g seaweed) and the pectase (seas 3000U/g is added Algae), 5h is digested, boiling water bath enzyme deactivation 10min, 8000r/m centrifuge 15min.Supernatant is 5KDa ultrafiltration membranes by molecular cut off Ultrafiltration, 80 DEG C of trapped fluid are concentrated into original volume 1/4, and 4 DEG C of absolute ethyl alcohols of precooling are added after cooling to ethyl alcohol final concentration 75%, take out Filter, precipitation freeze-drying obtain laver amylose and laminarin respectively.

The ingredient and its mass percentage for forming anti-dandruff shampoo of the present invention be：

D-Glucosamine Hydrochloride 0.01%, paphia undulata peptide 0.05%, tuna protein peptides 0.025%, seaweed Polysaccharide 0.01%, laminarin 0.02%, from Shanghai He Pu Industrial Co., Ltd.s buy piroctone olamine salt 0.5%, Myristic acid 4%, coconut oleoyl amine MEA6%, glycol distearate 3%, sodium cocoamphoacetate 10%, laruyl alcohol - 3 sodium sulfovinate of polyethers, Plantacare 818 5%, Cocoamidopropyl betaine 2.5%, polyquaternium -7 0.2%, DMDM second Interior uride 0.4%, vanillic aldehyde 1%, PEG-6 caprylic/caprics glyceride type 3%, glycerine 3%, deionized water surplus.

The anti-dandruff shampoo action temperature and foaming abundancy that present embodiment is prepared, can effectively clean hair and scalp, after washing It is comfortable on and loosens, fat secretion and pruritis substantially mitigate.After being used continuously 2 weeks, dandruff significantly reduces.

Embodiment 2

As described in Example 1, D-Glucosamine Hydrochloride is prepared.

It takes and meets the edible pearl shell soft body that China's correlation marine product uses standard, decladding adds 4 times after removing internal organ Volume pure water is homogenized, and alkali protease (3000U/g shellfish meats) is added, and is 7 in pH, temperature 50 C digests 4h, boiling water bath enzyme deactivation 10min, 8000r/m centrifuge 15min, and supernatant passes through retention point again by molecular cut off 3KDa ultrafiltration membrane ultrafiltration, filtered solution Pearl shell peptide is made in son amount 1KDa ultrafiltration membrane ultrafiltration, trapped fluid spray drying.

As described in Example 1, salmon protein peptides are prepared.

As described in Example 1, Hijiki polysaccharide is prepared.

The ingredient and its mass percentage for forming anti-dandruff shampoo of the present invention be：D-Glucosamine Hydrochloride 0.05%, pearl shell peptide 0.01%, salmon protein peptides 0.01%, Hijiki polysaccharide 0.02%, piroctone olamine salt 0.1%, Polyquaternium-10 0.25%, PEG-120 methyls dioleate 1.5%, laureth sulfosuccinate Disodium (mass fraction 40%) 2.5%, sodium cocoamphoacetate (mass fraction 39%) 7.7%, -3 sulphur of laureth Acid esters sodium (mass fraction 28%) 25%, -4/ Cocoamidopropyl betaine 1% of glycol distearate/laureth, Propylene glycol 1.5%, sodium chloride 0.45%, citric acid 0.03%, imidazolidinyl urea 0.2%, lauryl aldehyde 0.5%, more than deionized water Amount.

The anti-dandruff shampoo that present embodiment is prepared has both cleaning and conditioning effect, can effectively clean hair and scalp, Energy is anti-dandruff, antipruritic again, repairs impaired scalp cuticula and hair scale.Continuous use 2 weeks, hair is no longer greasy, becomes soft It is suitable, be easy to comb, it is glossy, dandruff disappear.

Embodiment 3

It takes and meets the edible scallop soft body that China's correlation marine product uses standard, decladding is gone after internal organ plus 4 times of bodies Product pure water homogenate, is added papain (3000U/g shellfish meats), alkali protease (3000U/g shellfish meats), is 7 in pH, temperature 50 DEG C, 4h is digested, boiling water bath enzyme deactivation 10min, 8000r/m centrifuge 15min, and supernatant is super by molecular cut off 3KDa ultrafiltration membranes Filter, for filtered solution again by molecular cut off 1KDa ultrafiltration membrane ultrafiltration, scallop peptide is made in trapped fluid spray drying.

As described in Example 1, tuna protein peptides, salmon protein peptides are made respectively.

As described in Example 1, spirulina polysaccharide is prepared.

The ingredient and its mass percentage for forming anti-dandruff shampoo of the present invention be：It is purchased from Hubei Ju Sheng Science and Technology Ltd.s The D-Glucosamine Hydrochloride 0.03% bought, scallop peptide 0.02%, tuna protein peptides 0.02%, salmon protein peptides 0.01%, spirulina polysaccharide 0.01%, piroctone olamine salt 0.75%, PEG-150 distearates 3%, glycerine 2%, Guar hydroxypropyltrimonium chloride 0.1%, citric acid 0.05%, PEG-80 anhydrous sorbitols laurate 13%, γ-purple Rowland ketone 0.5%, quaternary ammonium salt -15 0.1%, Polyquaternium-10 0.3%, Cocoamidopropyl betaine 25%, tridecanol Sodium sulfovinate 20%, lauroyl both sexes base diethyl acid disodium 5.5%, tetra- sodium 0.4% of EDTA, glycol distearate/bay - 4/ Cocoamidopropyl betaine 8% of alcohol polyethers, cyclomethicone 0.75%, deionized water surplus.

The anti-dandruff shampoo action temperature and irritation that present embodiment is prepared are small, in the same of effectively cleaning hair and scalp When, inhibit P.ovale growth, alleviate inflammation.Continuous use 2 weeks, dandruff, pruritis disappear, and hair becomes soft Easily comb.

Embodiment 4

As described in Example 1, oyster peptide is prepared.

The ingredient and its mass percentage for forming anti-dandruff shampoo of the present invention be：D-Glucosamine Hydrochloride 0.02%, oyster peptide 0.025%, the salmon protein peptides bought from Guangdong emerging hundred million marine biotechnology limited liability company 0.04%, the Hijiki polysaccharide 0.02% bought from Qingdao Bo Zhihui power bio tech ltd, from Xi'an Bai Chuan biologies section The spirulina polysaccharide 0.02% of skill Co., Ltd purchase, piroctone olamine salt 0.2%, from the limited public affairs of the bright refined trade of Ji in Guangzhou Take charge of purchase olive oil 0.5%, from hundred taste bio tech ltd of Hebei buy panthenol 0.3%, Plantacare 818 2%, The smooth isostearate 1% of PEG-160 sorbs, PEG-12 1%, Rylo MG10 1%, PEG-60 rilanit specials 0.5%, the moon Laureth sulfosuccinate acid disodium 8.2%, PEG-4 lauramides carboxylic acid sodium 8%, Cocoamidopropyl betaine 3%, carboxylic first Base cocoyl poly- propylamine sodium 1%, cis-jasmone 0.2%, citric acid 0.01%, gathers Bis(hydroxymethyl)imidazolidinyl urea 0.2% Quaternary ammonium salt -6 0.5%, deionized water surplus.

Anti-dandruff shampoo action temperature that present embodiment is prepared and, it is good, not dry and astringent to wash back hair comb rationality, and cleaning force is suitable In.After being used continuously 2 weeks, scalp no longer itch, no dandruff generates.

Embodiment 5

As described in Example 1, paphia undulata peptide and oyster peptide are prepared.

The ingredient and its mass percentage for forming anti-dandruff shampoo of the present invention be：D-Glucosamine Hydrochloride 0.04%, paphia undulata peptide 0.01%, oyster peptide 0.02%, the tuna bought from Shaanxi Jin Tai bioengineering Co., Ltd Protein peptides 0.05%, laver amylose 0.03%, the Hijiki polysaccharide bought from Qingdao Bo Zhihui power bio tech ltd 0.02%, piroctone olamine salt 0.4%, olive oil 0.1%, panthenol 0.5%, bay alcohol sulfuric ester ammonium 16%, laruyl alcohol - 3 sulfuric ester ammonium 6% of polyethers, BHT 0.1%, carbomer 0.8%, coconut oleoyl amine MEA13%, O-3-P 2.1%, guar hydroxypropyltrimonium chloride 0.3%, phenoxyethyl isobutyrate 0.2%, polysiloxane emulsion 8%, mica/ Titanium dioxide 0.3%, glycol distearate 0.1%, propylene glycol 1%, deionized water surplus.

It is comfortable, salubrious to wash back cutaneous sense for the anti-dandruff shampoo action temperature and moderate foam that present embodiment is prepared.Even It is continuous using after 2 weeks, scalp is no longer greasy, itch.

Embodiment 6

As described in Example 1, mussel peptide and pearl shell peptide are prepared.

The ingredient and its mass percentage for forming anti-dandruff shampoo of the present invention be：D-Glucosamine Hydrochloride 0.025%, mussel peptide 0.02%, pearl shell peptide 0.02%, tuna protein peptides 0.01%, salmon protein peptides 0.01%, from Laminarin 0.015%, spirulina polysaccharide 0.01%, the piroctone olamine of the Xi'an bio tech ltd Bai Chuan purchase Salt 0.6%, olive oil 0.3%, panthenol 0.1%, -3 sodium sulfovinate of laureth (mass fraction 28%) 35.7%, essence 0.3%, quaternary ammonium salt -80 2%, hexyl decyl alcohol/ceramide -3 0.5%, Phenoxyethanol/methyl hydroxybenzoate/Nipasol/oxybenzene Butyl ester 0.5%, Cocoamidopropyl betaine 7.3%, Cocoamidopropyl betaine/lauryl glucoside 5%, propylene glycol/ PEG-55 propylene glycol oleates 0.8%, ethylene glycol stearate/stereth -4 3%, sodium chloride 1%, to tertiary butyl ring Hexanol 0.2%, deionized water surplus.

The anti-dandruff shampoo that present embodiment is prepared can effectively clean hair and scalp, and it is comfortable to wash rear scalp, and hair combs Property is good.After being used continuously 2 weeks, no dandruff generates, and hair quality is obviously improved.

Embodiment 7

Anti-dandruff shampoo prepared by embodiment 1-6 carries out anti-dandruff efficacy assessments.

Test object is sample 1 (A, a), sample 2 (B, b), sample 3 (C, c), sample 4 (D, d), sample 5 (E, e), sample 6(F、f).Sample A-F be successively embodiment 1-6 prepare have effects that anti-dandruff shampoo, and sample a-f is sample A- successively (deionized water of sample a-f equivalent substitutes D-Glucosamine Hydrochloride, the seashells in sample A-F to the control sample of F Mass percentage shared by peptide, fish protein peptide, algal polysaccharides, piroctone olamine salt, olive oil and panthenol, i.e. sample a- D-Glucosamine Hydrochloride, seashells peptide, fish protein peptide, algal polysaccharides, piroctone olamine salt, olive are free of in f Olive oil and panthenol).

Choose have dandruff perplex 36 people of volunteer, age 18-48 Sui, be randomly divided into 12 groups (i.e. sample A, a, B, b, C, C, D, d, E, e, F, f group), every group of 3 people.It is had one's hair wash 1 time within every 2 days, is used continuously 4 weeks using counter sample.Using sample 0 day and 28 days, record dandruff scoring, itch scoring, measure scalp chlosma clump count, with detect sample whether have it is antibacterial, disappear Scorching, anti-dandruff, itching-relieving efficacies.

The scalp of every volunteer is divided into 6 regions (three areas before, during and after arranged on left and right sides takes respectively), using " glutinous Ten grades of point systems of the property dandruff " carry out dandruff scoring, i.e., 0 grade indicates no dandruff, and 1-2 grades are the greyish white rough scales of small flakes, 3-4 grades are as low as median size chip, 5-6 grade for the loose big and thin chip being connected of scalp, 7-8 grades are big tackness Chip, 9-10 grades are the white thicker scales of skin that peel off to yellow with scalp close attachment.Itch is carried out using 8 grades of itch point systems to comment Point, i.e., 0 grade indicates no itch, and 1-2 grades are slight itch, and 3-4 grades are moderate itch, and 5-6 grades are severe itch, and 7-8 grades are different Normal itch simultaneously has inflammatory sepage.Using the method for scouring, from each region area about 4cm²Scurf area materials, scurf, which is seeded to, to be contained The Sharpe plating medium of 5% sesame oil, 37 DEG C of constant temperature incubations 8 days record chlosma clump count.Each region dandruff scoring phase It is the dandruff scoring of volunteer to add gained, and each region itch scoring is added the itch scoring that gained is volunteer, each region Chlosma clump count is added the chlosma clump count that gained is volunteer.The scoring of each sample dandruff, itch scoring are pair The average value of volunteer's scoring should be organized, each sample chlosma clump count is being averaged for corresponding volunteer's chlosma clump count Value.Experimental result is as shown in table 1.

Table 1：Each sample is antibacterial, anti-inflammatory, anti-dandruff, itching-relieving efficacies test

Compared with sample a, b, c, d, e, f, as a result show using containing D-Glucosamine Hydrochloride, seashells peptide, fish Protein peptides, algal polysaccharides, piroctone olamine salt anti-dandruff shampoo after, dandruff scoring improve 2.83-3.13 times (about 17.4-19.0), itch scoring improves 2.68-2.97 times (about 15.3-17.5), chlosma clump count improves 5.58-7.77 times (25.2/cm²- 26.5/cm²).Using more containing D-Glucosamine Hydrochloride, seashells peptide, fish protein peptide, seaweed Sugar, piroctone olamine salt, olive oil, panthenol test specimen handled by scalp, dandruff scoring improve 3.73-3.96 times The scoring of (about 23.4-24.9), itch improves 3.59-3.83 times (about 21.5-20.2), chlosma clump count improves 5.03- 7.88 times (25.0/cm²- 28.2/cm²).Containing olive oil, panthenol can further cooperate with other active ingredients it is anti-dandruff, Itching-relieving efficacies.

This shows that the anti-dandruff shampoo of the present invention has antibacterial, anti-inflammatory, anti-dandruff, itching-relieving efficacies, thermophilic using scalp after 28 days Lipid chlosma significantly reduces, and itch, inflammation and dandruff significantly improve.

Embodiment 8

Following 3 samples are subjected to antistatic property and the test of hair surface frictional property：

1. hair composition matrix：- 3 sulfuric ester ammonium 6.5% of laureth, bay alcohol sulfuric ester ammonium 8.1%, laruyl alcohol Sodium sulfovinate 1.4%, coconut oleoyl amine MEA 1%, cetanol 0.35%, laruyl alcohol 0.2%, methyl [double-(h-tallow aliphatic radical Amide ethyl)] -2- hydroxyethyl methyl ammoniums sulfate 0.4%, trihydroxy stearin 0.25%, sodium chloride 0.3%, dimethylbenzene sulphur Sour ammonium 0.2%, sodium citrate 0.4%, sodium benzoate 0.25%, oleamide diemethylamine 0.1%, gathers citric acid 0.04% Decene 0.4%, trimethylolpropane 0.1%, deionized water surplus.

2. sample A：Containing D-Glucosamine Hydrochloride 0.01%, hair composition matrix surplus.

3. sample B：Containing D-Glucosamine Hydrochloride 0.05%, hair composition matrix surplus.

D-Glucosamine Hydrochloride is bought from Hubei Ju Sheng Science and Technology Ltd.s, and test environment relative humidity is 40%- 60%, the tested long 20cm of hair are all from the same female volunteer, 30 years old age, long hair, natural straight hair (being unstained).

Using surface specific resistance tester, with reference to standard GB/T/T 16801-1997 and GB/T 14342-93 to fabric The method that is measured of softening agent, evaluate anti-hair antistatic property with Δ lg ρ s (surface specific resistance drop), Δ lg ρ s are bigger, Show that antistatic property is better.

Using LDY type anti-static instruments, with reference to FZ/T 01042-1996 textile material antistatic property assay methods, with decaying Time T1/2 (s) evaluates anti-hair antistatic property, and half-life period is shorter, shows that antistatic property is better.

Friction factor μ of the hair after using sample, frictional resistance are measured using Y151 fiber type friction factor analyzers With its normal pressure ratio static friction factor μ s, the ratio between mean kinetic friction force and its normal pressure are dynamic friction factor μ d.Friction factor It is smaller, show that the flatness of hair is better, cardability is also better.

Each sample repeats to survey 3 times, takes its average value.Each sample antistatic property and hair surface frictional property test result are such as Shown in table 2.

Table 2:Each sample antistatic property and the test of hair surface frictional property

After using the sample (sample A, sample B) containing D-Glucosamine Hydrochloride, hair surface specific resistance drop is aobvious It writes and increases, and electrostatic half-life, static friction factor and dynamic friction factor are substantially reduced.This shows be added to D- aminoglucoses After sugared hydrochloride, the antistatic property of hair be improved significantly, suface friction significantly reduces.That is, D- amino Portugal Grape sugar hydrochloride has hair excellent film forming, hair with reduced friction to generate electrostatic, moreover it is possible to protect hair, repair and be damaged hair Scale promotes smoothness, makes it easy to comb.

Embodiment 9

Following 7 samples are subjected to bacteriostasis efficacy evaluation.

Malassezia furfur ATCC44344 (is protected purchased from Guangzhou promise such as Instrument Ltd. from Guangdong microorganism fungus kind Tibetan center), sterile distilled water is purchased from Guangzhou Watson food and drink Co., Ltd.

D-Glucosamine Hydrochloride, seashells peptide, fish protein peptide and algal polysaccharides are made as described in Example 1.

1. piroctone olamine salt aqueous solution (content is mass fraction 0.5%, and surplus is sterile distilled water)

2. water-soluble containing D-Glucosamine Hydrochloride, seashells peptide, fish protein peptide, algal polysaccharides, olive oil and panthenol Liquid (i.e. sample A, total content are mass fraction 0.5%, and surplus is sterile distilled water)

A1：D-Glucosamine Hydrochloride 0.05%, oyster peptide 0.05%, tuna protein peptides 0.05%, laver amylose 0.02%, laminarin 0.03%, olive oil 0.2%, panthenol 0.1%, sterile distilled water surplus；

A2：D-Glucosamine Hydrochloride 0.01%, paphia undulata peptide 0.01%, pearl shell peptide 0.01%, salmon Protein peptides 0.02%, Hijiki polysaccharide 0.01%, olive oil 0.14%, panthenol 0.3%, sterile distilled water surplus；

A3：D-Glucosamine Hydrochloride 0.03%, scallop peptide 0.01%, mussel peptide 0.01%, tuna protein peptides 0.02%, salmon protein peptides 0.02%, spirulina polysaccharide 0.05%, olive oil 0.16%, panthenol 0.2%, sterile distilled water Surplus.

3. containing D-Glucosamine Hydrochloride, seashells peptide, fish protein peptide, algal polysaccharides, piroctone olamine salt, Olive oil and panthenol aqueous solution (i.e. sample B, total content are mass fraction 0.5%, and surplus is sterile distilled water)

B1：D-Glucosamine Hydrochloride 0.01%, oyster peptide 0.05%, tuna protein peptides 0.03%, laver amylose 0.01%, laminarin 0.02%, piroctone olamine salt 0.18%, olive oil 0.1%, panthenol 0.1%, sterile distilled water Surplus；

B2：D-Glucosamine Hydrochloride 0.05%, paphia undulata peptide 0.01%, pearl shell peptide 0.01%, salmon Protein peptides 0.01%, Hijiki polysaccharide 0.02%, piroctone olamine salt 0.1%, olive oil 0.2%, panthenol 0.1%, nothing Bacterium distilled water surplus；

B3：D-Glucosamine Hydrochloride 0.03%, scallop peptide 0.02%, mussel peptide 0.03%, tuna protein peptides 0.02%, salmon protein peptides 0.03%, spirulina polysaccharide 0.05%, piroctone olamine salt 0.12%, olive oil 0.1%, panthenol 0.1%, sterile distilled water surplus.

It is prepared by print：Sterile dry filter paper piece is taken, 20 μ L sample solution are added dropwise respectively, lies against the sterile empty plate of cleaning It is interior, it is set in Biohazard Safety Equipment spare after spontaneously drying at room temperature.Negative control group is distilled water.

Bacteriostatic experiment：In the 3rd generations of Malassezia furfur ATCC44344 that recovery was identified, are inoculated in Leeming-Hotman Culture medium flat plate, 30 DEG C of culture 24-48h.Bacteria suspension is made with sterile saline in picking colony, is dipped with sterile cotton swab dense Degree is 1 × 10⁴cfu/mL-9×10⁴Cfu/mL Malassezia furfur ATCC44344 suspensions are flat in Leeming-Hotman culture mediums Plate surface is uniformly smeared 3 times.It often smears 1 time, tablet rotates 60 °, finally cotton swab is used to be smeared 1 week around plate edge.It covers flat Ware, the dry 5min in Biohazard Safety Equipment.Then, it is placed in planar surface with aseptic nipper coupongs, each print center is apart 25mm or more, with tablet periphery at a distance of 15mm or more.After being placed with, with the light pressure-like piece of aseptic nipper, it is made to be close to planar surface. Plate is covered, after setting 30 DEG C of mold incubator culture 48h, with vernier caliper measurement inhibition zone diameter and is recorded.Each sample weight Repetition measurement 3 times, test result is as shown in table 3.

Table 3：Each sample antibacterial circle diameter (mm)

As shown in Table 3, be equally 5% concentration active constituent, the antibacterial circle diameter of sample B is noticeably greater than bacteriostatic agent pyrrole The antibacterial circle diameter of Rock ketone ethanolamine salt aqueous solution, although illustrating that piroctone olamine salt is commonly used for as anti-dandruff Agent inhibits the growth of microorganism, and still, fungistatic effect is limited when being used alone, and the present invention uses D- glucosamine salts Hydrochlorate, seashells peptide, fish protein peptide, algal polysaccharides, olive oil and panthenol are remarkably improved the suppression of piroctone olamine salt Bacterium activity prompts have good synergistic function between them.Still more, in active component of the invention, piroctone Mass percent of the ethanolamine salt in system is less than 0.2%, 0.5% piroctone olamine salt aqueous solution of insufficient bacteriostatic agent Half, this also from the composition that the present invention has been reacted in side, is not only piroctone olamine salt and plays bacteriostasis efficacy, but The Multiple components such as D-Glucosamine Hydrochloride, seashells peptide, fish protein peptide, algal polysaccharides cooperate with performance as a whole Bacteriostasis efficacy.

Embodiment 10

Following each sample is subjected to scalp, hair quality improves efficacy assessments.

1. shampoo substrate：- 3 sulfuric ester ammonium 6.5% of laureth, bay alcohol sulfuric ester ammonium 8.1%, laruyl alcohol sulfuric acid Ester sodium 1.4%, coconut oleoyl amine MEA 1%, cetanol 0.35%, laruyl alcohol 0.2%, methyl [double-(h-tallow aliphatic radical amide Ethyl)] -2- hydroxyethyl methyl ammoniums sulfate 0.4%, trihydroxy stearin 0.25%, sodium chloride 0.3%, ammonium xylene sulfonate 0.2%, citric acid 0.04%, sodium citrate 0.4%, sodium benzoate 0.25%, oleamide diemethylamine 0.1%, poly decene 0.4%, trimethylolpropane 0.1%, deionized water surplus.

2. containing piroctone olamine salt (content is mass fraction 0.5%, and surplus is shampoo substrate)

3. containing D-Glucosamine Hydrochloride, seashells peptide, fish protein peptide and algal polysaccharides, (i.e. sample A, total content are Mass fraction 0.5%, surplus are shampoo substrate)

A1：D-Glucosamine Hydrochloride 0.05%, oyster peptide 0.15%, tuna protein peptides 0.1%, laver amylose 0.1%, laminarin 0.1%, shampoo substrate surplus；

A2：D-Glucosamine Hydrochloride 0.05%, paphia undulata peptide 0.1%, pearl shell peptide 0.1%, salmon egg White peptide 0.15%, Hijiki polysaccharide 0.1%, shampoo substrate surplus；

A3：D-Glucosamine Hydrochloride 0.1%, scallop peptide 0.1%, mussel peptide 0.1%, tuna protein peptides 0.05%, salmon protein peptides 0.05%, spirulina polysaccharide 0.1%, shampoo substrate surplus.

4. containing D-Glucosamine Hydrochloride, seashells peptide, fish protein peptide, algal polysaccharides and piroctone olamine salt (i.e. sample B, total content are mass fraction 0.5%, and surplus is shampoo substrate)

B1：D-Glucosamine Hydrochloride 0.01%, oyster peptide 0.05%, tuna protein peptides 0.03%, laver amylose 0.01%, laminarin 0.02%, piroctone olamine salt 0.38%, shampoo substrate surplus；

B2：D-Glucosamine Hydrochloride 0.05%, paphia undulata peptide 0.01%, pearl shell peptide 0.01%, salmon Protein peptides 0.01%, Hijiki polysaccharide 0.02%, piroctone olamine salt 0.4%, shampoo substrate surplus；

B3：D-Glucosamine Hydrochloride 0.03%, scallop peptide 0.02%, mussel peptide 0.03%, tuna protein peptides 0.02%, salmon protein peptides 0.03%, spirulina polysaccharide 0.05%, piroctone olamine salt 0.32%, shampoo substrate Surplus.

5. containing olive oil and panthenol (i.e. sample C, total content are mass fraction 0.5%, and surplus is shampoo substrate)

C1：Olive oil 0.3%, panthenol 0.2%, shampoo substrate surplus；

C2：Olive oil 0.4%, panthenol 0.1%, shampoo substrate surplus.

6. containing D-Glucosamine Hydrochloride, seashells peptide, fish protein peptide, algal polysaccharides, piroctone olamine salt, Olive oil and panthenol (i.e. sample D, total content are mass fraction 0.5%, and surplus is shampoo substrate)

D1：D-Glucosamine Hydrochloride 0.01%, oyster peptide 0.05%, tuna protein peptides 0.03%, laver amylose 0.01%, laminarin 0.02%, piroctone olamine salt 0.18%, olive oil 0.1%, panthenol 0.1%, shampoo substrate Surplus；

D2：D-Glucosamine Hydrochloride 0.05%, paphia undulata peptide 0.01%, pearl shell peptide 0.01%, salmon Protein peptides 0.01%, piroctone olamine salt 0.1%, olive oil 0.2%, panthenol 0.1%, are washed Hijiki polysaccharide 0.02% The matrix that becomes visible surplus；

D3：D-Glucosamine Hydrochloride 0.03%, scallop peptide 0.02%, mussel peptide 0.03%, tuna protein peptides 0.02%, salmon protein peptides 0.03%, spirulina polysaccharide 0.05%, piroctone olamine salt 0.12%, olive oil 0.1%, panthenol 0.1%, shampoo substrate surplus.

Volunteer 39 is chosen, male 21, female 18, age 25-52 Sui, skin of head is without ox-hide moss, atopic dermatitis The equal specific disease patient of clinical diagnosises is as subject.13 groups, i.e. shampoo substrate group are randomly divided into, piroctone olamine is contained Salt group, sample A groups (sample A1-A3 contains D-Glucosamine Hydrochloride, seashells peptide, fish protein peptide and algal polysaccharides), sample (sample B1-B3 contains D-Glucosamine Hydrochloride, seashells peptide, fish protein peptide, algal polysaccharides and piroctone second to product B groups Alcohol amine salt), sample C groups (sample C1-C2, containing olive oil and panthenol), sample D (sample D1-D3, glucosamine hydrochloric acid containing D- Salt, seashells peptide, fish protein peptide, algal polysaccharides, piroctone olamine salt, olive oil and panthenol), every group 3.Every 2 days It is had one's hair wash 1 time using sample, if any using other hair cosmetics that must not have effects that antidandruff, is used continuously 4 weeks.

Compare and uses sample 0 day and 28 days, the fat content of cuticula, moisture, hair friction factor, hair unit Glossiness difference, to detect improvement of each sample to scalp, hair.Multi probe Multi-functional skin tester is respectively adopted MPA 10 (German CK companies production) carries out oil to the scalp of tester and moisture detects.Using Y151 fiber type frictions Factor analyzer measures hair friction factor μ, and value is smaller, shows that hair flatness is better, cardability is also better.It is bright using hair Damp instrument is measured hair gloss, and value is bigger, shows that hair gloss is better.

Each sample repeats to survey 3 times, takes its average value.Sample A is the average value of sample A1-A3 groups, and sample B is sample The average value of B1-B3 groups, sample C are the average value of sample C1-C2 groups, and sample D is the average value of sample D1-D3 groups.Each sample Test result is as shown in table 4.

Table 4：Each sample improves efficacy assessments to scalp, hair

Compared with shampoo substrate group, after using other samples 4 weeks, scalp cuticula moisture increases, grease point Reduction is secreted, hair becomes smooth, glossy.

(contain D-Glucosamine Hydrochloride, seashells peptide, Fish protein with containing pyrroles gram ketone ethanolamine salt group and sample A Peptide and algal polysaccharides) it compares, sample B (contains D-Glucosamine Hydrochloride, seashells peptide, fish protein peptide, algal polysaccharides and pyrrole Rock ketone ethanolamine salt) increase scalp cuticula moisture, reduce fat secretion and improve hair quality effect it is more notable.This explanation Piroctone olamine salt has good association with D-Glucosamine Hydrochloride, seashells peptide, fish protein peptide, algal polysaccharides Same synergistic effect, the anti-dandruff shampoo containing these substances have effects that lasting moisturizing, Bergamot Mint Extract and improve hair quality.

(contain D-Glucosamine Hydrochloride, seashells peptide, fish protein peptide, algal polysaccharides and piroctone second with sample B Alcohol amine salt) it is compared (containing olive oil and panthenol) with sample C, sample D is (containing D-Glucosamine Hydrochloride, seashells peptide, fish egg White peptide, algal polysaccharides, piroctone olamine salt, olive oil and panthenol) increase that scalp cuticula moisture, to improve hair soft Compliance and luster effect are most notable.This illustrates D-Glucosamine Hydrochloride, seashells peptide, fish protein peptide, algal polysaccharides, pyrrole Rock ketone ethanolamine salt has good synergistic function with olive oil, panthenol, the anti-dandruff shampoo tool containing these substances There are significantly persistently moisturizing, Bergamot Mint Extract, repair scalp stratum corneum barrier and improves hair quality effect.

It can be seen that the anti-dandruff shampoo of the present invention has apparent moisturizing, Bergamot Mint Extract, antibacterial, anti-inflammatory, goes Bits, antipruritic, reparation stratum corneum barrier, improvement hair quality effect.

The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment Limitation, it is other it is any without departing from the spirit and principles of the present invention made by changes, modifications, substitutions, combinations, simplifications, All without departing from present disclosure, it is included in protection scope of the present invention.

Claims

1. a kind of composition, it is characterised in that contain D-Glucosamine Hydrochloride；The composition is for protecting hair or system The product of standby protection hair.

2. composition according to claim 1, it is characterised in that the D-Glucosamine Hydrochloride is preparing protection Mass percentage content in the product of hair is 0.01%-0.1%；Preferably 0.01%-0.05%.

3. a kind of composition, it is characterised in that the composition contains D-Glucosamine Hydrochloride, seashells peptide, Fish protein Peptide, algal polysaccharides and piroctone olamine salt.

4. composition as claimed in claim 3, it is characterised in that the component containing following weight fraction：1-10 parts of D- amino Portugals Grape sugar hydrochloride, 1-10 parts of seashells peptides, 1-10 parts of fish protein peptides, 1-10 parts of algal polysaccharides and 10-100 parts of piroctone second Alcohol amine salt；Preferably, the component containing following weight fraction：1-5 parts of D-Glucosamine Hydrochlorides, 1-5 parts of seashells peptides, 1-5 parts of fish protein peptides, 1-5 parts of algal polysaccharides and 10-75 parts of piroctone olamine salts.

5. composition as claimed in claim 3, it is characterised in that the composition also contains olive oil and panthenol；

Preferably, the olive oil containing 10-100 parts by weight；The further preferably olive oil of 10-50 parts by weight；

Preferably, the panthenol containing 10-100 parts by weight；The further preferably panthenol of 10-50 parts by weight.

6. composition according to claim 3, it is characterised in that the component containing following parts by weight：

7. the composition as described in claim 3 or 6, it is characterised in that the seashells peptide from paphia undulata, The combination of one or more of pearl shell, scallop, mussel and oyster.

8. the composition as described in claim 3 or 6, it is characterised in that the fish protein peptide derives from tuna and/or three Literary fish.

9. the composition as described in claim 3 or 6, it is characterised in that the algal polysaccharides are from seaweed, sargassum fusifome, sea The combination of one or more of band, spirulina, bulk kelp, yellow tang, bladder-wrack.

10. the composition as described in claim 1-9 is any, it is characterised in that the composition is used to prepare or as shield Send out product；Preferably, it is used to prepare or is used as shampoo；Preferably, it is used to prepare or is used as anti-dandruff shampoo；

Preferably, weight percent of each component in shampoo is D-Glucosamine Hydrochloride 0.01%- in the composition 0.1%, seashells peptide 0.01%-0.1%, fish protein peptide 0.01%-0.1%, algal polysaccharides 0.01%-0.1%, pyrrole Rock Ketone ethanolamine salt 0.1%-1.0%；

It is highly preferred that weight percent of each component in shampoo is in the composition：D-Glucosamine Hydrochloride 0.01%-0.05%, seashells peptide 0.01%-0.05%, fish protein peptide 0.01%-0.05%, algal polysaccharides 0.01%- 0.05%, piroctone olamine salt 0.1%-0.75%；

Or preferably, weight percent of each component in shampoo is D-Glucosamine Hydrochloride in the composition 0.01%-0.1%, seashells peptide 0.01%-0.1%, fish protein peptide 0.01%-0.1%, algal polysaccharides 0.01%- 0.1%, piroctone olamine salt 0.1%-1.0%, olive oil 0.1%-1.0%, panthenol 0.1%-1.0%；

It is highly preferred that weight percent of each component in shampoo is D-Glucosamine Hydrochloride in the composition 0.01%-0.05%, seashells peptide 0.01%-0.05%, fish protein peptide 0.01%-0.05%, algal polysaccharides 0.01%- 0.05%, piroctone olamine salt 0.1%-0.75%, olive oil 0.1%-0.5%, panthenol 0.1%-0.5%.